CN108362690A - A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood - Google Patents
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood Download PDFInfo
- Publication number
- CN108362690A CN108362690A CN201810022894.2A CN201810022894A CN108362690A CN 108362690 A CN108362690 A CN 108362690A CN 201810022894 A CN201810022894 A CN 201810022894A CN 108362690 A CN108362690 A CN 108362690A
- Authority
- CN
- China
- Prior art keywords
- red sandalwood
- sample
- test solution
- dyestuff
- sandalwood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses a kind of methods of quick discriminating red sandalwood and dyestuff red sandalwood, including following operating procedure：(1) it takes red sandalwood and the pretreatment sample of dyestuff red sandalwood to be separately added into methanol ultrasonic extraction respectively, obtains the test solution of red sandalwood test solution and dyestuff red sandalwood respectively；(2) petroleum ether, ethyl acetate and acetic acid mixing, obtain solvent；(3) distinguish the test solution of the red sandalwood test solution and dyestuff red sandalwood in aspiration step (1), point sample is then placed in solvent obtained by step (2) and impregnates in same silica gel plate the same horizontal position successively；(4) take out silica obtained plate drying after being impregnated in step (3), be placed in iodine cylinder develop the color to get.Raw material needed for the present invention is less, greatly reduces the damage to craftwork or sample；Easy to operate, common experimental conditions can be met the requirements, and not need cumbersome experimental procedure and or by large-scale experiment equipment；Operating time is short.
Description
Technical field
The present invention relates to redwood and blackwood products discriminating field is differentiated, more particularly to one kind is quick differentiates red sandalwood and contaminates
Expect the method for red sandalwood.
Background technology
Red sandalwood (Pterocarpus santalinus L.f.) is subordinate to pulse family Pterocarpus, and main product is in India's Mysore
Nation is a kind of rare redwood seeds.Since red sandalwood growth is slower, become a useful person than sparser, and wooden hard, color and luster
It is magnificent, become the various furniture of making, the choice wood of craftwork causes red sandalwood timber due to its high economic value
Excessive use, resource is gradually deficient, is put into Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) annex II control.
Dyestuff red sandalwood (Pterocarpus tinctorius Welw.) is commonly called as " blood wingceltis " or " Zambia blood wingceltis ", is mainly distributed on non-
The ground such as Zambia, the Congo and the Tanzania in continent.Due to the similitude with red sandalwood microstructure characteristic etc., illegal businessman's warp
Often use it for Counterfeit Item as red sandalwood, very disruptive redwood market is suffered greatly to consumer and operator
Economic loss.
Due to the two macroscopic view and the similitude of microtexture, greatly difficulty is caused to traditional wood anatomy technology, and
Slice is big to sample broke amount, needs abundant professional knowledge in addition, increasingly seeks other efficiently simple discriminating means.Such as
Coke is vertical superfine to be delivered《A kind of miniature DNA bar code and its discrimination method and application differentiating red sandalwood and dyestuff red sandalwood》,
By being extracted to DNA and by pcr amplification reaction and sequencing technologies, carrying out sequence alignment analysis, examined from molecular biology
The discriminating that the angle of survey is set out to two kinds of redwood, but this method relies on PCR Lab, and experimental procedure is relatively cumbersome.Xia Zhaopeng
Etc. delivering《The discrimination method of red sandalwood and dyestuff red sandalwood timber and its product》, by ultrasonic extraction, by GC-MS combined instruments
Extracting solution ingredient is analyzed, the difference of two kinds of redwood is compared.
Being disclosed in the information of the background technology part, it is only intended to increase understanding of the overall background of the invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or imply that the information is constituted in any form.
Invention content
The present invention differentiates that red sandalwood is difficult to differentiate between with being sliced in the method for dyestuff red sandalwood for the prior art, experiment condition
More harsh or needs differentiate that the complex and high cost problem of discrimination method invents a kind of quick and convenient mirror by large-scale instrument
The method of other red sandalwood and dyestuff red sandalwood.
To achieve the above object, technical solution provided by the invention is as follows：
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, including following operating procedure：
(1) it takes red sandalwood and the pretreatment sample of dyestuff red sandalwood to be separately added into methanol ultrasonic extraction respectively, obtains wingceltis respectively
The test solution of fragrant red sandalwood test solution and dyestuff red sandalwood；
(2) petroleum ether, ethyl acetate and acetic acid mixing, obtain solvent；
(3) test sample with red sandalwood test solution and dyestuff red sandalwood in capillary difference aspiration step (1) is molten
Liquid, successively point sample be then placed in solvent obtained by step (2) and impregnate in same silica gel plate the same horizontal position；
(4) take out silica obtained plate drying after being impregnated in step (3), be placed in iodine cylinder develop the color to get；Two kinds of test samples are molten
Agent point obtained speckle displacement and number on lamellae have apparent difference.
Wherein, the dosage mass ratio of the pretreatment sample described in step (1) and methanol is 1:4~79, preferably institute
The dosage mass ratio of the pretreatment sample and methanol stated is 1:4~24.
Wherein, the pretreatment sample dosage described in step (1) is 0.005~0.05g, and pretreatment sample is red sandalwood
Pretreatment sample and dyestuff red sandalwood pretreatment sample, each plant demand are 0.005~0.05g.
Wherein, the dosage of the methanol described in step (1) is 0.2376~0.792g.
Wherein, the ultrasonic extraction time described in step (1) is 20~40min, and the frequency of the ultrasound is 45KHZ.
Wherein, the general granularity of pretreatment sample described in step (1) is uniform as possible, and small as possible using container diameter,
Solvent did not had sample powder.
Wherein, the dosage of the petroleum ether described in step (2), ethyl acetate and acetic acid is according to petroleum ether：Ethyl acetate：
Acetic acid=10:(1~8.0):0.1~0.6 volume ratio mixes, it is preferred that the use of the petroleum ether, ethyl acetate and acetic acid
Amount is according to petroleum ether：Ethyl acetate：Acetic acid=10:(2.5~3.0):0.3 volume ratio mixes.
Wherein, the volume for being put into solvent described in step (2) should depending on expansion cylinder size, highly for 1.0~
1.5cm being advisable.
Wherein, the red sandalwood test solution described in step (3) and the respective dosage of dyestuff red sandalwood test solution
It is all 1~1.5 μ L, exhibition is away from for 5~9cm.
Wherein, the spacing of the point sample described in step (3) is 1.0~2.0cm.
Compared with prior art, the present invention has the advantages that：
(1) raw material needed for is less, greatly reduces the damage to craftwork or sample；
(2) easy to operate, common experimental conditions can be met the requirements, and not need cumbersome experimental procedure and or by large size
Experimental facilities；
(3) operating time is short, differentiates that work terminates only to need 1h from being sampled to.
Description of the drawings
Fig. 1 is the colour developing figure of 1 red sandalwood of embodiment and dyestuff red sandalwood in same silica GF254 lamellae.
Fig. 2 is the colour developing figure of 2 red sandalwood of embodiment and dyestuff red sandalwood in same silica GF254 lamellae.
Fig. 3 is the colour developing figure of 3 red sandalwood of embodiment and dyestuff red sandalwood in same silica GF254 lamellae.
Specific implementation mode
It is described in detail With reference to embodiment, it is to be understood that protection scope of the present invention is not by specific
The limitation of embodiment, the silica gel plate used in embodiment is commercial silica gel GF254Lamellae.
Embodiment 1
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, operating procedure are as follows：
(1) it uses cutter to scrape laminar sample respectively from red sandalwood and dyestuff red sandalwood sample, that is, respectively obtains pre-
Sample is handled, according to pretreatment sample and methanol at mass ratio 1:47.52 take red sandalwood to pre-process sample with dyestuff red sandalwood respectively
Each 0.005g of product, is each placed in the glass container of diameter 1.0cm and is sealed, and the methanol for being then respectively adding 0.2376g is adopted
Ultrasonic extraction 20min is sealed with frequency 45KHZ, obtains red sandalwood test solution and dyestuff red sandalwood test solution respectively；Its
In, the general granularity of pretreatment sample is uniform as possible, and small as possible using container diameter, and solvent did not had sample powder；
(2) according to petroleum ether：Ethyl acetate：Acetic acid=10:2.7:0.3 volume ratio, with pipette measure petroleum ether 10mL,
Ethyl acetate 2.7mL and 300 μ L of acetic acid are mixed in expansion cylinder, obtain solvent, the volume of solvent is according to expansion cylinder size
Depending on, it is highly 1.0~1.5cm；
(3) with the red sandalwood test solution and dyestuff red sandalwood test solution in capillary difference aspiration step (1)
Each 1.3 μ L, point sample is in same silica G F successively254Lamellae the same horizontal position, the distance between sample spot are about 1.2cm, so
After be put into step (2) and impregnated in gained solvent, exhibition is away from for 9cm；
(4) silica obtained GF after being impregnated in step (3) is taken out254Lamellae dry up, be placed in iodine cylinder develop the color to get；Two kinds
Test sample point solvent obtained speckle displacement and number on lamellae have apparent difference, and phenomenon is as shown in Figure 1, perpendicular
The upward left side point of histogram is red sandalwood, and the point on the right is dyestuff red sandalwood.
Embodiment 2
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, operating procedure are as follows：
(1) it uses cutter to scrape laminar sample respectively from red sandalwood and dyestuff red sandalwood sample, that is, respectively obtains pre-
Sample is handled, according to pretreatment sample and methanol at mass ratio 1:4.752 take red sandalwood to pre-process sample with dyestuff red sandalwood respectively
Each 0.05g of product, is each placed in the glass container of diameter 1.0cm and is sealed, and the methanol for being then respectively adding 0.2376g uses
Frequency 45KHZ seals ultrasonic extraction 40min, obtains red sandalwood test solution and dyestuff red sandalwood test solution respectively；Wherein,
The general granularity of pretreatment sample is uniform as possible, and small as possible using container diameter, and solvent did not had sample powder；
(2) according to petroleum ether：Ethyl acetate：Acetic acid=10:2.5:0.3 volume ratio, with pipette measure petroleum ether 10mL,
Ethyl acetate 2.5mL and 300 μ L of acetic acid are mixed in expansion cylinder and impregnate, and obtain solvent, the volume of solvent is according to expansion cylinder
It is highly 1.0~1.5cm depending on size；
(3) with the red sandalwood test solution and dyestuff red sandalwood test solution in capillary difference aspiration step (1)
Each 1.5 μ L, point sample is in same silica G F successively254Lamellae the same horizontal position, the distance between sample spot are about 1.4cm, so
After be put into step (2) in gained solvent, exhibition is away from for 8.6cm；
(4) silica obtained GF after being impregnated in step (3) is taken out254Lamellae dry up, be placed in iodine cylinder develop the color to get；Two kinds
Test sample point solvent obtained speckle displacement and number on lamellae have apparent difference, and phenomenon is as shown in Fig. 2, perpendicular
The upward left side point of histogram is red sandalwood, and the point on the right is dyestuff red sandalwood.
Embodiment 3
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, operating procedure are as follows：
(1) it uses cutter to scrape laminar sample respectively from red sandalwood and dyestuff red sandalwood sample, that is, respectively obtains pre-
Sample is handled, according to pretreatment sample and methanol at mass ratio 1:23.76 take red sandalwood to pre-process sample with dyestuff red sandalwood respectively
Each 0.01g of product, is each placed in the glass container of diameter 1.0cm and is sealed, and the methanol for being then respectively adding 0.2376g uses
Frequency 45KHZ seals 20~40min of ultrasonic extraction, obtains red sandalwood test solution and dyestuff red sandalwood test solution respectively；
Wherein, the general granularity of pretreatment sample is uniform as possible, and small as possible using container diameter, and solvent did not had sample powder；
(2) according to petroleum ether：Ethyl acetate：Acetic acid=10:3.0:0.3 volume ratio, with pipette measure petroleum ether 10mL,
Ethyl acetate 3.0mL and 300 μ L of acetic acid are mixed in expansion cylinder and impregnate, and obtain solvent, the volume of solvent is according to expansion cylinder
It is highly 1.0~1.5cm depending on size；
(3) with the red sandalwood test solution and dyestuff red sandalwood test solution in capillary difference aspiration step (1)
Each 1.5 μ L, point sample is in same silica G F successively254Lamellae the same horizontal position, the distance between sample spot are about 1.2cm, so
After be put into step (2) in gained solvent, exhibition is away from for 9cm；
(4) silica obtained GF after being impregnated in step (3) is taken out254Lamellae dry up, be placed in iodine cylinder develop the color to get；Two kinds
Test sample point solvent obtained speckle displacement and number on lamellae have apparent difference, and phenomenon is as shown in figure 3, perpendicular
The upward left side point of histogram is red sandalwood, and the point on the right is dyestuff red sandalwood.
Embodiment 4
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, operating procedure are as follows：
(1) it uses cutter to scrape laminar sample respectively from red sandalwood and dyestuff red sandalwood sample, that is, respectively obtains pre-
Sample is handled, according to pretreatment sample and methanol at mass ratio 1:79.2 take red sandalwood and dyestuff red sandalwood pretreatment sample respectively
Each 0.01g is each placed in the glass container of diameter 1.0cm and is sealed, and is then respectively adding the methanol of 0.792g using frequency
Rate 45KHZ seals ultrasonic extraction 20min, obtains red sandalwood test solution and dyestuff red sandalwood test solution respectively；Wherein, in advance
It is uniform as possible to handle the general granularity of sample, and small as possible using container diameter, solvent did not had sample powder；
(2) according to petroleum ether：Ethyl acetate：Acetic acid=10:1:0.1 volume ratio measures petroleum ether 10mL, second with pipette
Acetoacetic ester 1.0mL and 100 μ L of acetic acid are mixed in expansion cylinder, obtain solvent, the volume of solvent according to expansion cylinder size and
It is fixed, it is highly 1.0~1.5cm；
(3) with the red sandalwood test solution and dyestuff red sandalwood test solution in capillary difference aspiration step (1)
Each 1.0 μ L, point sample is in same silica G F successively254Lamellae the same horizontal position, the distance between sample spot are about 1.0cm, so
After be put into step (2) and impregnated in gained solvent, exhibition is away from for 5cm；
(4) silica obtained GF after being impregnated in step (3) is taken out254Lamellae dry up, be placed in iodine cylinder develop the color to get.
Embodiment 5
A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, operating procedure are as follows：
(1) it uses cutter to scrape laminar sample respectively from red sandalwood and dyestuff red sandalwood sample, that is, respectively obtains pre-
Sample is handled, according to pretreatment sample and methanol at mass ratio 1:47.52 red sandalwood is taken to pre-process sample with dyestuff red sandalwood respectively
Each 0.005g of product, is each placed in the glass container of diameter 1.0cm and is sealed, and the methanol for being then respectively adding 0.2376g is adopted
Ultrasonic extraction 20min is sealed with frequency 45KHZ, obtains red sandalwood test solution and dyestuff red sandalwood test solution respectively；Its
In, the general granularity of pretreatment sample is uniform as possible, and small as possible using container diameter, and solvent did not had sample powder；
(2) according to petroleum ether：Ethyl acetate：Acetic acid=10:8.0:0.6 volume ratio, with pipette measure petroleum ether 10mL,
Ethyl acetate 8.0mL and 600 μ L of acetic acid are mixed in expansion cylinder, obtain solvent, the volume of solvent is according to expansion cylinder size
Depending on, it is highly 1.0~1.5cm；
(3) with the red sandalwood test solution and dyestuff red sandalwood test solution in capillary difference aspiration step (1)
Each 1.3 μ L, point sample is in same silica G F successively254Lamellae the same horizontal position, the distance between sample spot are about 2.0cm, so
After be put into step (2) and impregnated in gained solvent, exhibition is away from for 7cm；
(4) silica obtained GF after being impregnated in step (3) is taken out254Lamellae dry up, be placed in iodine cylinder develop the color to get.
Raw material needed for the present invention is less, it is only necessary to which 0.005~0.05g greatly reduces the damage to craftwork or sample；Behaviour
Make simply, common experimental conditions can be met the requirements, and not need cumbersome experimental procedure and or by large-scale experiment equipment；Operation
Time is short, differentiates that work terminates only to need 1h from being sampled to.
The description of the aforementioned specific exemplary embodiment to the present invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining the specific principle of the present invention and its actually answering
With so that those skilled in the art can realize and utilize the present invention a variety of different exemplary implementation schemes and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (10)
1. a kind of method of quick discriminating red sandalwood and dyestuff red sandalwood, which is characterized in that include following operating procedure：
(1) it takes red sandalwood and the pretreatment sample of dyestuff red sandalwood to be separately added into methanol ultrasonic extraction respectively, it is purple to obtain santal respectively
The test solution of wingceltis test solution and dyestuff red sandalwood；
(2) petroleum ether, ethyl acetate and acetic acid mixing, obtain solvent；
(3) test solution of the red sandalwood test solution and dyestuff red sandalwood in aspiration step (1) respectively, successively point sample in
Same silica gel plate the same horizontal position is then placed in solvent obtained by step (2) and impregnates；
(4) take out silica obtained plate drying after being impregnated in step (3), be placed in iodine cylinder develop the color to get.
2. according to the method described in claim 1, it is characterized in that：The use of pretreatment sample and methanol described in step (1)
It is 1 to measure mass ratio:4~79.
3. according to the method described in claim 1, it is characterized in that：Pretreatment sample dosage described in step (1) is 0.005
~0.05g.
4. according to the method described in claim 1, it is characterized in that：The dosage of methanol described in step (1) be 0.2376~
0.792g。
5. according to the method described in claim 1, it is characterized in that：Ultrasonic extraction time described in step (1) is 20~
The frequency of 40min, the ultrasound are 45KHZ.
6. according to the method described in claim 1, it is characterized in that：The general granularity of pretreatment sample described in step (1) is most
Amount is uniform, and small as possible using container diameter, and solvent did not had sample powder.
7. according to the method described in claim 1, it is characterized in that：Petroleum ether, ethyl acetate and acetic acid described in step (2)
Dosage be according to petroleum ether：Ethyl acetate：Acetic acid=10:(1~8.0):0.1~0.6 volume ratio mixes.
8. according to the method described in claim 1, it is characterized in that：The volume for being put into solvent described in step (2) answers root
Depending on expansion cylinder size, highly it is advisable for 1.0~1.5cm.
9. according to the method described in claim 1, it is characterized in that：Red sandalwood test solution described in step (3) and
The respective dosage of dyestuff red sandalwood test solution is all 1~1.5 μ L, and exhibition is away from for 5~9cm.
10. according to the method described in claim 1, it is characterized in that：The spacing of point sample described in step (3) be 1.0~
2.0cm。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810022894.2A CN108362690A (en) | 2018-01-10 | 2018-01-10 | A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810022894.2A CN108362690A (en) | 2018-01-10 | 2018-01-10 | A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108362690A true CN108362690A (en) | 2018-08-03 |
Family
ID=63011319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810022894.2A Pending CN108362690A (en) | 2018-01-10 | 2018-01-10 | A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108362690A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111157507A (en) * | 2020-03-05 | 2020-05-15 | 南京市产品质量监督检验院 | Method for identifying pterocarpus santalinus wood species by fluorescence spectrum |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380298A (en) * | 2002-04-26 | 2002-11-20 | 林文翰 | Method for separating and extracting total flavone from goldenrain tree plant and its application |
| CN101057895A (en) * | 2005-10-26 | 2007-10-24 | 北京奇源益德药物研究所 | 'Fuyanshu' preparation for treating gynopathy and its preparation method and quality controlling method and application |
| CN101279066A (en) * | 2008-05-28 | 2008-10-08 | 陕西思壮药业有限公司 | Quality testing method of medicament composition for curing hysteromyoma of gynecology |
| CN101279067A (en) * | 2008-02-28 | 2008-10-08 | 张会林 | Preparation of shaolin plaster for rheumatism and wound and quality control method |
| CN102091168A (en) * | 2009-12-11 | 2011-06-15 | 天津宏仁堂药业有限公司 | Quality control method for Chinese medicine preparation Xuefuzhuyu capsule |
| CN102293827A (en) * | 2011-04-14 | 2011-12-28 | 江西天施康中药股份有限公司 | Quality detection method for camphor tree root and preparation containing camphor tree root |
| CN102359996A (en) * | 2011-05-13 | 2012-02-22 | 云南大唐汉方制药有限公司 | Quality detection method for dranaena cochinchinensis |
| CN103399120A (en) * | 2013-07-30 | 2013-11-20 | 广州白云山和记黄埔中药有限公司 | Thin-layer identification method of medicine linearstripe rabdosia herb |
| CN104045648A (en) * | 2013-03-13 | 2014-09-17 | 中国科学院大连化学物理研究所 | Method for large-scale preparation of wedelolactone chemical reference substance |
| CN104893989A (en) * | 2015-05-29 | 2015-09-09 | 浙江工业大学 | Rhizopus microsporus root-shaped variant ZJPH1308 and application thereof in preparation of sitagliptin intermediate |
| CN105572263A (en) * | 2016-02-26 | 2016-05-11 | 夏兆鹏 | Identification method of pterocarpus santalinus wood and pterocarpus tinctorius wood and products of pterocarpus santalinus wood and pterocarpus tinctorius wood |
| CN105699584A (en) * | 2016-02-03 | 2016-06-22 | 昆药集团股份有限公司 | Detection method for artemether related matters |
| CN105925506A (en) * | 2016-05-27 | 2016-09-07 | 浙江工业大学 | Pseudomonas aeruginosa ZJPH1504 and application thereof in preparation of sitagliptin chiral intermediate |
-
2018
- 2018-01-10 CN CN201810022894.2A patent/CN108362690A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1380298A (en) * | 2002-04-26 | 2002-11-20 | 林文翰 | Method for separating and extracting total flavone from goldenrain tree plant and its application |
| CN101057895A (en) * | 2005-10-26 | 2007-10-24 | 北京奇源益德药物研究所 | 'Fuyanshu' preparation for treating gynopathy and its preparation method and quality controlling method and application |
| CN101279067A (en) * | 2008-02-28 | 2008-10-08 | 张会林 | Preparation of shaolin plaster for rheumatism and wound and quality control method |
| CN101279066A (en) * | 2008-05-28 | 2008-10-08 | 陕西思壮药业有限公司 | Quality testing method of medicament composition for curing hysteromyoma of gynecology |
| CN102091168A (en) * | 2009-12-11 | 2011-06-15 | 天津宏仁堂药业有限公司 | Quality control method for Chinese medicine preparation Xuefuzhuyu capsule |
| CN102293827A (en) * | 2011-04-14 | 2011-12-28 | 江西天施康中药股份有限公司 | Quality detection method for camphor tree root and preparation containing camphor tree root |
| CN102359996A (en) * | 2011-05-13 | 2012-02-22 | 云南大唐汉方制药有限公司 | Quality detection method for dranaena cochinchinensis |
| CN104045648A (en) * | 2013-03-13 | 2014-09-17 | 中国科学院大连化学物理研究所 | Method for large-scale preparation of wedelolactone chemical reference substance |
| CN103399120A (en) * | 2013-07-30 | 2013-11-20 | 广州白云山和记黄埔中药有限公司 | Thin-layer identification method of medicine linearstripe rabdosia herb |
| CN104893989A (en) * | 2015-05-29 | 2015-09-09 | 浙江工业大学 | Rhizopus microsporus root-shaped variant ZJPH1308 and application thereof in preparation of sitagliptin intermediate |
| CN105699584A (en) * | 2016-02-03 | 2016-06-22 | 昆药集团股份有限公司 | Detection method for artemether related matters |
| CN105572263A (en) * | 2016-02-26 | 2016-05-11 | 夏兆鹏 | Identification method of pterocarpus santalinus wood and pterocarpus tinctorius wood and products of pterocarpus santalinus wood and pterocarpus tinctorius wood |
| CN105925506A (en) * | 2016-05-27 | 2016-09-07 | 浙江工业大学 | Pseudomonas aeruginosa ZJPH1504 and application thereof in preparation of sitagliptin chiral intermediate |
Non-Patent Citations (5)
| Title |
|---|
| FANG-DA ZHANG ET AL.: "Rapid identification of Pterocarpus santalinus and Dalbergia louvelii by FTIR and 2D correlation IR spectroscopy", 《JOURNAL OF MOLECULAR STRUCTURE》 * |
| 侯世海: "檀香、紫檀香、降香及苏木的紫外光谱和薄层色谱鉴别", 《青海医药杂志》 * |
| 唐丽娟等: "薄层层析在植物黄酮类化合物研究中的应用", 《时珍国医国药》 * |
| 李凯: "薄层色谱方法的改进", 《实验室科学》 * |
| 梅萍等: "檀香紫檀和染料紫檀快速微损鉴别方法", 《福建农林大学学报（自然科学版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111157507A (en) * | 2020-03-05 | 2020-05-15 | 南京市产品质量监督检验院 | Method for identifying pterocarpus santalinus wood species by fluorescence spectrum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Doménech-Carbó et al. | Identification of synthetic resins used in works of art by Fourier transform infrared spectroscopy | |
| EP0016387A1 (en) | Diagnostic means for the determination of components of liquids | |
| CN101766240B (en) | Method for judging pile fermentation degree of Pu'er tea | |
| CN108362690A (en) | A kind of method of quick discriminating red sandalwood and dyestuff red sandalwood | |
| CN106116693A (en) | A kind of high-strength interfacial agents of composite polymer emulsion being applicable to vitrified tile and preparation | |
| AU5422890A (en) | Method and apparatus for capillary hydrodynamic fractionation | |
| Vinciguerra et al. | Structural analysis of lignin in chestnut wood by pyrolysis-gas chromatography/mass spectrometry | |
| CN105588907B (en) | The method that micro- damage based on finger-print differentiates Barry yellow wingceltis kind | |
| CN107607634A (en) | The method of chemical root resistance agent in quick discriminating chemistry root-resistant type root-resistant puncturing material | |
| CN105486615B (en) | Test card of agricultural chemicals ultra-low volume finish droplet density and size and preparation method thereof | |
| Jabeen et al. | Russula rubricolor sp. nov. from Himalayan forests of Pakistan | |
| Stahl et al. | Thermofractography of lignins and its use for rapid analysis on the ultra micro-scale | |
| CN105588906A (en) | Method for identifying pterocarpus macrocarpus species in microdamage mode based on fingerprint spectrum | |
| Long et al. | Matrix solid phase dispersion (MSPD) extraction and liquid chromatographic determination of five benzimidazole anthelmintics in pork muscle tissue | |
| CN106187705B (en) | A kind of method that purifying from krill prepares DBPC 2,6 ditertiary butyl p cresol | |
| CN105132541A (en) | Method, special primer and probe for identifying DNA (deoxyribonucleic acid) of siam rosewood payung among rosewood | |
| CN104729905B (en) | The preparation method of anti-coagulants, anti-coagulants and prepare the method for blood smear using it | |
| CN103884709B (en) | Portable food safety detector | |
| CN106324116B (en) | A kind of the HPLC characteristic spectrums detection method and collection of illustrative plates of Lagotis brachystachya Maxim | |
| CN108169376A (en) | A kind of method of Rapid identification Wuyi cliff tea and its tea perfume fine work matter | |
| CN104830319B (en) | A kind of containing double rare earth Eu3+/ Tb3+the nanometer zeolite L luminescent material of-beta diketone coordination compound and application thereof | |
| CN101871919B (en) | Method for distinguishing bee glue and poplar glue | |
| CN105588908B (en) | The method that micro- damage based on finger-print differentiates hedgehog red sandalwood kind | |
| WO2016001979A1 (en) | Automatic sample introduction apparatus, sample introduction method, and program | |
| CN110184359A (en) | A kind of method of quick detection heredity skin-whitening ability gene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180803 |
|
| RJ01 | Rejection of invention patent application after publication |