CN108359577A - Zebra fish-egg rotation micro OS based on micro fluid dynamcis and control method - Google Patents
Zebra fish-egg rotation micro OS based on micro fluid dynamcis and control method Download PDFInfo
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Abstract
A kind of zebra fish-egg rotation micro OS and control method based on micro fluid dynamcis, the system body are a micro-fluidic chip, enter microchannel equipped with two liquid flows at an angle in the micro-fluidic chip and liquid flows out microchannel, a Cytology Lab；The Cytology Lab side wall enters microchannel with liquid flow and liquid outflow microchannel interlinks；The area of section that the liquid flow enters microchannel is less than the area of section that liquid flows out microchannel.When control fluid enters microchannel with certain speed from liquid flow flows out microchannel through Cytology Lab side wall influent, fluid driving forces band kinetocyte automatically generates rotary motion, the cell speed of rotation and angle can control speed and the time of fluid flowing by high-accuracy syringe pump, realize automatic adjustment.Present system performance accuracy height, high degree of automation, repeatability are strong, cheap, easy to operate, assist determining injection site using present system, have higher positioning accuracy, therefore can greatly improve microinjection success rate.
Description
Technical field
The invention belongs to micro-nano operating technology fields, are related to a kind of micro- behaviour of zebra fish-egg rotation based on micro fluid dynamcis
Make system and control method.
Background technology
Zebra fish is a kind of important model organism, has high similarity on structure, biochemistry level with human organ,
It is widely applied in the research of embryonic development mechanism and the research of various human diseases.Zebrafish embryo is in vitro fertilization, body
It is outer development and embryo it is transparent, therefore observe and operate very convenient.Allogenic material importing is carried out for zebrafish embryo cell, into
And carry out correlated inheritance, auxology experimental study is the very important research contents of current field of biology, and microinjection
Technology is to realize one of the important enabling tool of the research, is a very universal operating method.But due to fish egg cell
Anisotropy and injection mass difference, when injection generally requires to select different injection sites that could realize expectation function,
Therefore one of the key difficulties of injection are that fish-egg how to be made to rotate to a suitable angle to determine injection site.
For cell pose regulation problem, it can realize that the mainstream technology of controllable adjustment is broadly divided into contact and non-at present
Contact two major classes.Wherein, contact micromanipulation method is to carry out sticking to cell by micro manipulator tool, is being discharged repeatedly
Accidental result in find suitable pose, the experience for the operator that places one's entire reliance upon, success rate is very low, and is easily caused to cell
Damage.And contactless operating method is then directly acted on by certain " power " or " torque " on cell, it is allowed to move or rotate,
Such as laser method, dielectrophoresis method, electromagnetic method；Or allow solution residing for cell to generate microfluidic, drive cell mobile with this or
Rotation.Both operating methods can avoid or reduce the injury to cell, be current research mainstream both domestic and external.
In non-contact type cell pose adjusting method, microfluid method is pressure, the viscous force generated using fluid motion
For principle Deng driving cell movement come what is operated, key technology is how to generate and control the fortune of fluid under micro-scale
It moves, i.e. micro flow field.Institutes Of Technology Of Nanjing discloses a kind of single cell microoperation apparatus for microscopic injection, and (Chinese Patent Application No. is
CN200810019091), which can make the micro- of cell rotation by fixing two out-of-alignment glass-micropipe injections
Fluid controls the rotational angle of cell by controlling the speed and flow of fluid, is a kind of safe operating method.But if
Size, the size variation of cell, when solution viscosity is different, need to fixed two glass-micropipes and its flow into
Row, which is readjusted, even to be redesigned.Japan Electric communication university reports a kind of by vibrating glass microbot generation swirling flow field
Method will be rotated when cell is in this swirling flow field.This method will not cause cell mechanical wounding, but
When in micron and sub-micrometer range, the mode of vibration of glass-micropipe will be subject to certain restrictions, and the adjustment of cell posture is made to have one
It settles finally sex-limited.It can be avoided completely to cell damage using the method that micro flow field adjusts cell posture, what is proposed at present can
The method for generating micro flow field is both needed in structure, by glass-micropipe, cause design complicated, and adjustment is inconvenient, and the degree of automation is not high,
Therefore the parallel adjusting for being not suitable for high-volume cell, has certain gap apart from functionization.
Invention content
Aiming at the above shortcomings existing in the prior art and disadvantage, the purpose of the present invention is to provide one kind being based on microfluid
The zebra fish-egg rotation micro OS and control method of driving, system operatio precision height, high degree of automation, the repeatability
By force, cheap, easy to operate, it can assist realizing the operation of high-precision cell microinjection, for zebra fish-egg and its allogene
The accurate transfection of cell and development observational study are of great significance.
In order to solve above-mentioned technical problem, the present invention is achieved by the following technical solutions：
A kind of zebra fish-egg rotation micro OS based on micro fluid dynamcis, which is a micro-fluidic chip,
Enter microchannel equipped with two liquid flows at an angle in the micro-fluidic chip and liquid flows out microchannel, a cell
Room；The Cytology Lab side wall enters microchannel with liquid flow and liquid outflow microchannel interlinks；The liquid flow enters microchannel
Area of section is less than the area of section of liquid outflow microchannel.
When control fluid enters microchannel with certain speed from liquid flow flows out microchannel through Cytology Lab side wall influent,
Fluid driving forces band kinetocyte automatically generates rotary motion, and the cell speed of rotation and angle can be controlled by high-accuracy syringe pump to flow
The speed of body flowing and time, realize automatic adjustment.
According to one embodiment, the micro-fluidic chip is being equipped with groove below the Cytology Lab.
According to one embodiment, the Cytology Lab is 3/4 cylinder, and the section of 3/4 cylinder is top surface thereon.
According to one embodiment, the liquid, which flows out microchannel and with the liquid flow enter microchannel to be placed in homonymy and intersect, is in
Certain angle；It is to be arranged along the tangential direction of Cytology Lab minimum point that the liquid flow, which enters microchannel, and at point of contact and Cytology Lab
It is connected, the section that the liquid flow enters microchannel is circle；The liquid outflow microchannel is top surface and arc along Cytology Lab
The tangential direction of face intersection is arranged, and is connected with Cytology Lab at point of contact, and the section of the liquid outflow microchannel is circle
Shape.
According to one embodiment, the liquid flow enters microchannel and is intersected at river conjunction with liquid outflow microchannel；It is described
River conjunction is a hollow cylinder, and the liquid flow enters microchannel from passing through among it, and the liquid flows out microchannel phase therewith
Connection；The area of section of the river conjunction is all higher than the area of section and liquid outflow micro-pipe that the liquid flow enters microchannel
The sum of the area of section in road.
According to one embodiment, the micro-fluidic chip uses polymer poly dimethyl siloxane (PDMS) material.
A kind of control method of zebra fish-egg rotation micro OS based on micro fluid dynamcis, this method content packet
It includes：Micro OS is rotated by the zebra fish-egg based on micro fluid dynamcis, can realize the zebra fish for waiting for microinjection
The controllable adjustment of egg cell pose changes the inflow velocity and angle of driving microfluid, it will be able to which accurate adjustment zebra fish-egg is thin
The pose of born of the same parents finds targeted injection site；Concrete methods of realizing is as follows：First, zebra fish egg cell to be regulated is put into carefully
Cell, observation are injected the initial position in site, judge that required application drives the angle of fluid flow direction, miniflow is then arranged
Body inflow velocity is higher than critical incipient motion flow rate Fstart, make zebra fish egg cell start quickly to rotate, slowly adjusting flow velocity later makes
Zebra fish egg cell smooth rotation simultaneously slowly reduces rotating speed, makes it easy to microscopic observation and software calculates；Finally, it is adjusted precisely to
Required pose.
Due to the adoption of the above technical scheme, a kind of zebra fish-egg based on micro fluid dynamcis provided by the invention rotates micro- behaviour
Make system and control method, there is such advantageous effect compared with prior art：
Present system is a kind of automation micro OS for biologic grains such as zebra fish egg cells, passes through the rotation
Turn microoperation micro-fluidic chip, it can be achieved that waiting for the controllable adjustment of the zebra fish egg cell pose of microinjection, it is only necessary to change and drive
The inflow velocity and angle of dynamic microfluid, you can accurate adjustment zebra fish egg cell pose finds targeted injection site, Neng Goutong
It crosses simple fluid motion control zebra fish egg cell and generates rotation process within the scope of certain angle, it is easy to operate effective, it can
The quick locating injection site of microinjector is assisted, there is preferable operability, stability and automaticity.With showing for no positioning
Microinjection is compared, and present system performance accuracy height, high degree of automation, repeatability are strong, cheap, easy to operate, use
Present system auxiliary determines injection site, has higher positioning accuracy, therefore can greatly improve microinjection success rate.
Further, since present invention employs biocompatibility and the preferable PDMS material of the transparency, rotation zebra fish-egg cannot be only used for
The posture of cell, moreover it is possible to realize the culture and hatching of zebra fish egg cell inside it, combined high precision microscopic observation and micro-
Injecting systems can complete a series of research and development tasks such as the manipulation to zebra fish egg cell, transfection and development observation, have larger
Versatility.
Description of the drawings
Fig. 1 is micro- rotary chip structural plan schematic diagram；
Fig. 2 is micro- rotary chip structural upright schematic diagram；
Fig. 3 is fish egg cell Principle of Rotating；
Fig. 4 is zebra fish egg cell rotary working platform；
Fig. 5 is the relation schematic diagram of fish egg cell rotary speed and micro- rotary chip fluid velocity.
Specific implementation mode
It is further illustrated the present invention below in conjunction with the accompanying drawings with example.
A kind of one embodiment of zebra fish-egg rotation micro OS based on micro fluid dynamcis of the present invention, structure
Floor map and stereoscopic schematic diagram difference are as depicted in figs. 1 and 2, which is a micro-fluidic chip, described micro-fluidic
Enter microchannel 2 equipped with two liquid flows at an angle in chip and liquid flows out microchannel 3, a Cytology Lab 1 is described thin
1 side wall of cell enters microchannel 2 with liquid flow and liquid outflow microchannel 3 interlinks；The section that the liquid flow enters microchannel is
It is round；The section of the liquid outflow microchannel is circle.
The Cytology Lab is 3/4 cylinder that diameter of section is 2.2mm for placing zebra fish egg cell, the Cytology Lab
Structure.Design scheme using cylindrical shape is considered for hydrodynamics, since arcuate structure is also easy to produce stronger anaclasis
And reflex, the imaging of cylindrical structure is ambiguous.Experiments have shown that the structure is easy that microfluid is made to produce inside it
Raw fluid whirl generates rapid circular movement to the zebra fish egg cell of driving diameter about 1mm in sufficient space.But by
It is also easy to produce stronger anaclasis and reflex in arcuate structure, is unfavorable for optical imagery.And 3/4 cylinder design then makes carefully
Cell has a smooth upper surface, optical imagery interference problem is reasonably being efficiently solved within sweep of the eye, to subtract
Few optical imagery interference, simultaneously because Cytology Lab height limitation and reduce movement of the cell in Z-direction, contribute to micro- focusing at
Picture.
Liquid outflow microchannel and liquid flow enter microchannel and are placed in homonymy and intersect at an angle.Two microchannels
It is connected with external macroscopical runner, length depends on chip appearance and size.Since duct length does not manipulate rotary chip
Behavior impacts, therefore does not need special designing.
The liquid flow enters microchannel, and liquid inflow direction is designed as tangential direction and cell along Cytology Lab minimum point
Room is connected, and cross-section of pipeline diameter is determined according to the microsyringe needle sizes of its connection, designs its diameter of section herein
For 0.45mm.The direction that the direction that liquid flows in pipeline can penetrate microchannel by syringe needle is suitably adjusted.
The liquid flows out microchannel, and liquid outflow conduit direction is likewise provided as the top surface along Cytology Lab and is handed over cambered surface
Tangential direction at remittance is connected with Cytology Lab, and circular pipe cross-sectional diameter is more slightly larger than flow ipe size, is designed as
0.55mm.Its purpose is to enough torque is generated in Cytology Lab to drive cell to rotate.
Since fluid effuser road can intersect with flow ipe in same plane, in/out liquid flow in order to prevent
Dynamic cross jamming, therefore this river conjunction is designed, the liquid flow enters microchannel and is intersected at river conjunction with liquid outflow microchannel；
The river conjunction is the hollow cylinder structure that a section circular diameter is 1.2mm, and liquid flow enters microchannel from passing through among it, liquid
Body outflow microchannel communicates therewith.Since than two entry/exit microchannel diameter of sections of its internal diameter are all big, outflow flow also can
Unhinderedly by flowing out microchannel.Such simple designs ingenious can avoid difference caused by two microchannels intersect and flow to fluid
Interactive interference problem.
The micro-fluidic chip is being equipped with groove below the Cytology Lab.Groove design below Cytology Lab is also
In the reliable imaging observation the considerations of, this structure can reduce refraction and scattering of the light in PDMS, greatly shorten light in PDMS
Material by distance help to obtain the image that is more clear, size can root to weaken light refraction and scattering phenomenon
It is determined according to actual conditions, is designed here as 5mm × 5mm.
The micro-fluidic chip is prepared by the molded method processing of PDMS.Microfluidic chip structure reticle design is flexible,
Size adjusting is convenient, and reusable.Since PDMS chips can realize prepared by batch machining, single micro-fluidic chip at
This is very cheap.Micro-fluidic chip preparation process is carried out according to following steps：
(1) template is processed：Using hard material polymethyl methacrylate (polymethylmethacrylate, PMMA,
It is commonly called as acrylic) it is used as template substrate material, carry out micro-structure processing using high-precision engraving machine.According to designed chip knot
Structure processes the formpiston that can be used for injection molding.
(2) PDMS cast moldings：Silica gel base and curing agent in proportion 10:1 mixing, casts in PMMA formpistons after stirring evenly
On, cure 1~2 day at room temperature, it is to be packaged after demoulding.
(3) tubing envelopes：To ensure fluid proper flow, open microchannel is packaged using glass substrate, foundation
Following step carries out：
1. washing away the organic contaminations of glass sheet surface with ethanol solution, and dry up；
2. treated sheet glass and PDMS substrates are put into plasma cleaner, by air plasma to glass
Piece and PDMS substrate surfaces carry out activation process, and technological parameter is：Pumpdown time 3min adjusts pressure time 2min, radio frequency
Power 90W, build-up of luminance time 2min, air mass flow 40sccm；
3. the glass substrate after corona treatment is bonded with PDMS precisions, and 1h is heated on 80 DEG C of hot plates；
4. finally micro-fluidic chip is taken out and checks bond strength.
Fig. 3 illustrates the principle that fish egg cell rotates in micro-fluidic chip.
It is the illustraton of model of zebra fish egg cell in Cytology Lab, arrow direction is liquid in microchannel and the indoor stream of cell
To.It is inevitable along thin into after Cytology Lab through analysis it is found that the liquid with certain flow rate enters microchannel inflow from liquid flow
Born of the same parents' edge voids flow fast through, and to generate tangential torque, liquid flows out micromanipulator along liquid outflow microchannel later, on a small quantity
Liquid also can continue circumnutation in Cytology Lab along cell edges.The torque given birth to by liquid flow movable property can drive cell with one
Constant speed rate rotates, and the speed of rotation is mainly influenced by flow rate of liquid.It should be noted that cell, which starts circling behavior, needs one
Fixed liquid initial velocity, initial flow rate is too small, cell cannot be driven to rotate.The initial velocity can be passed through according to the liquid of use
Test of many times determination is crossed, and with preferable repeatability.In addition, the present invention test link it has been proved that using deionized water or
Fish egg cell culture solution can complete the controllable rotating operation of zebra fish egg cell well.It is flowed as driving using culture solution
Body, contributes to fish egg cell to keep activated state during rotation process, does not influence other micro- behaviour after pose is adjusted
Make.Each material composition of culture solution is as follows：5mmol/L NaCl, 0.17mmol/L KCl, 0.33mmol/L CaCl2,
0.33mmol/L MgSO4。
The plateform system that needs when Fig. 4 gives present invention normal work, include mainly syringe pump, optical microphotograph observation and
Computer generated image records system three parts.Wherein, syringe pump is used to control the fluid velocity of injection rotation microoperation chip, and
Stepped flow rate control can be achieved；Optical microphotograph observation system for observing zebra fish egg cell in rotating microoperation chip in real time
Form, position and motion conditions；Image after microscopic observation is then transferred to terminal by CCD camera and data line,
It preserved, recorded and data analysis.In Fig. 4,6 be binary channels programmable infusion pump；7 be 5ml standard injector for medical purpose；8
For the medical use liquid connection flexible pipe with syringe needle, one end connects syringe, and other end connection rotation microoperation chip liquid flow is in a subtle way
Entrance；9 indicate that zebra fish egg cell rotates microoperation chip, are fixed on light microscope articles holding table；10 be band CCD phases
The light microscope of machine；11 be the video data line for connecting CCD and host computer USB port；12 be terminal.The platform
System ensure that the normal work of zebra fish egg cell rotation microoperation chip, cooperation Video processing software can accurately calculate spot
The rotary speed of horse fish egg cell, and establish between zebra fish egg cell rotary speed and flow rate of liquid or liquid viscosity coefficient
Relationship, to evaluate the working performance of rotation microoperation chip.
Fig. 5 gives the relation curve signal of rotary speed and fluid-flow rate of the fish-egg in rotating microoperation chip
The process for manipulating fish-egg rotation is divided into 4 stages by figure here.1. being quiescent phase, in this stage, fluid flow rate is opened from 0
Beginning is gradually increased to a critical value i.e. FstartBefore, zebra fish egg cell is stationary always, and only flow velocity is more than Fstart
Afterwards, fish-egg just starts to rotate with certain rotating speed, FstartReferred to as initial velocity.If being driving fluid, the starting stream with deionized water
Fast FstartAbout 0.3ml/min.2. the stage is manipulated for Linear Rotation, at this point, flow velocity is more than Fstart, zebra fish egg cell
Rotary speed is substantially linear related to flow velocity, i.e., water velocity is bigger, and the rotating speed of zebra fish egg cell is bigger.3. to start rotation
Rotating speed gently turns the stage down after turning.In in this stage, adjustment fluid velocity is less than Fstart, remain to that zebra fish egg cell is made to keep
Rotation status, and rotating speed is less than starting speed.Experiment is found, can make the minimum speed R of zebra fish egg cell uniform rotationmin
About 1r/s corresponds to fluid velocity F at this timeminAbout 0.1~0.15ml/min.4. absolute rest stage, this stage fluid
Speed is less than Fmin, tangential liquid driven that zebra fish egg cell cannot slowly be flowed and stop operating, revolved to restart
Turn, then needs fluid velocity being increased again to FstartOn.
Experiments have shown that rotation micro OS using the present invention can complete the high-precision to zebra fish egg cell pose,
The rotation manipulation of high stable, high repeatability.Structure of the invention designs simple and flexible, low processing cost, mode of operation simplicity
Effectively, single zebra fish egg cell can be controlled and generate circling behavior within the scope of certain angle, for the positioning of microinjection
It is of great significance.In addition, since processing method of the present invention is suitble to mass manufacture, it is thin to be easily achieved multiple zebra fish-eggs
The parallel manipulation and adjusting of born of the same parents, is greatly improved operating efficiency.
Claims (12)
1. a kind of zebra fish-egg based on micro fluid dynamcis rotates micro OS, it is characterised in that：The system body is one micro-
Fluidic chip, the micro-fluidic chip is interior to enter microchannel and liquid outflow microchannel equipped with two liquid flows at an angle,
One Cytology Lab；The Cytology Lab side wall enters microchannel with liquid flow and liquid outflow microchannel interlinks；The liquid flows into
The area of section of microchannel is less than the area of section of liquid outflow microchannel.
2. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 1 rotates micro OS, feature exists
In：The micro-fluidic chip is being equipped with groove below the Cytology Lab.
3. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 2 rotates micro OS, feature exists
In：The groove size is 5mm × 5mm.
4. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 1 rotates micro OS, feature exists
In：The Cytology Lab is 3/4 cylinder, and the section of 3/4 cylinder is top surface thereon.
5. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 4 rotates micro OS, feature exists
In：A diameter of 2.2mm of 3/4 cylinder of the Cytology Lab.
6. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 1 rotates micro OS, feature exists
In：Liquid outflow microchannel and the liquid flow enter microchannel and are placed in homonymy and intersect at an angle；The liquid flow
It is the tangential direction setting along Cytology Lab minimum point to enter microchannel, and is connected with Cytology Lab at point of contact, and the liquid flows into
The section of microchannel is circle；The liquid outflow microchannel is that top surface and the tangential direction of cambered surface intersection are set along Cytology Lab
It sets, and is connected with Cytology Lab at point of contact, the section of the liquid outflow microchannel is circle.
7. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 6 rotates micro OS, feature exists
In：The a diameter of 0.45mm of liquid flow pipeline section in a subtle way；The liquid outflow microchannel diameter of section is 0.55mm.
8. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 1 rotates micro OS, feature exists
In：The liquid flow enters microchannel and is intersected at river conjunction with liquid outflow microchannel；The river conjunction is a hollow cylinder,
The liquid flow enters microchannel from passing through among it, and the liquid outflow microchannel is attached thereto logical；The section of the river conjunction
Area is all higher than the sum of the area of section that the liquid flow enters the area of section and liquid outflow microchannel of microchannel.
9. a kind of zebra fish-egg based on micro fluid dynamcis according to claim 1 rotates micro OS, feature exists
In：A diameter of 1.2mm of the river conjunction cylinder.
10. according to a kind of zebra fish-egg rotation microoperation system based on micro fluid dynamcis of claim 1~9 any one of them
System, it is characterised in that：The micro-fluidic chip uses polymer polydimethyl siloxane material.
11. rotating micro OS according to a kind of zebra fish-egg based on micro fluid dynamcis of claim 1~9 any one of them
Control method, this method content includes：Pass through the zebra fish-egg slewing maneuver chip based on micro fluid dynamcis, Neng Goushi
It now waits for the controllable adjustment of the zebra fish egg cell pose of microinjection, changes the inflow velocity and angle of driving microfluid, energy
Enough accurate adjustment cell poses, find targeted injection site；Concrete methods of realizing is as follows：First, by fish egg cell to be regulated
It being put into particle and places microchamber, observation is injected the initial position in site, judges the required angle for applying driving fluid flow direction,
Then setting microfluid inflow velocity is higher than critical incipient motion flow velocity, and fish egg cell is made to start quickly to rotate, and slowly adjusts stream later
Speed makes fish-egg smooth rotation and slowly reduces rotating speed, makes it easy to microscopic observation and software calculates；Finally, needed for being adjusted precisely to
The pose wanted.
12. a kind of controlling party of zebra fish-egg rotation micro OS based on micro fluid dynamcis according to claim 10
Method, this method content include：By the zebra fish-egg slewing maneuver chip based on micro fluid dynamcis, can realize wait for it is micro-
The controllable adjustment of the zebra fish egg cell pose of injection changes the inflow velocity and angle of driving microfluid, it will be able to accurate to adjust
Whole cell pose, finds targeted injection site；Concrete methods of realizing is as follows：First, fish egg cell to be regulated is put into particle
Microchamber is placed, observation is injected the initial position in site, judges the required angle for applying driving fluid flow direction, is then arranged
Microfluid inflow velocity is higher than critical incipient motion flow velocity, and fish egg cell is made to start quickly to rotate, and slowly adjusting flow velocity later makes fish-egg
Smooth rotation simultaneously slowly reduces rotating speed, makes it easy to microscopic observation and software calculates；Finally, it is adjusted precisely to required position
Appearance.
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| CN109706054A (en) * | 2019-01-04 | 2019-05-03 | 哈尔滨工业大学 | The automatic conveying of zebra fish juvenile fish and the micro runner device of adjustment posture |
| CN109730799A (en) * | 2019-01-04 | 2019-05-10 | 哈尔滨工业大学 | The automatic conveying of zebra fish juvenile fish and adjustment attitude system |
| CN112452362A (en) * | 2020-09-25 | 2021-03-09 | 中山大学 | Pump-free fixed zebra fish microfluidic chip system and preparation method and application thereof |
| CN112716650A (en) * | 2021-01-25 | 2021-04-30 | 南开大学 | Robotized zebra fish main vein microinjection method |
| CN112986335A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Process for fixing high molecular substance on chip |
| CN113528583A (en) * | 2021-07-15 | 2021-10-22 | 澳门大学 | Automatic microinjection method, device, system, equipment and storage medium |
| CN114107025A (en) * | 2021-11-30 | 2022-03-01 | 平顶山学院 | Fluid driving method based on algae cells |
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