CN108353785A - A kind of orchid mating system - Google Patents
A kind of orchid mating system Download PDFInfo
- Publication number
- CN108353785A CN108353785A CN201711110503.4A CN201711110503A CN108353785A CN 108353785 A CN108353785 A CN 108353785A CN 201711110503 A CN201711110503 A CN 201711110503A CN 108353785 A CN108353785 A CN 108353785A
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- China
- Prior art keywords
- orchid
- culture
- seedling
- root
- mating system
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a kind of orchid mating systems, belong to field of plant cultivation seedling technology, are concretely a kind of methods of the sterile seedling rooting seedling of orchid, including orchid takes root, hardening, transplant step, beneficial effects of the present invention：（1）Orchid tissue-cultured seedling plant through the invention, taking root, fast, rooting rate is high.（2）Orchid tissue-cultured seedling robust plant, the easy transplant survival taken root using this method.（3）The orchid mating system of the present invention is easy to operate, at low cost, is suitable for the quick breeding of most ornamental orchids.
Description
Technical field
The invention belongs to field of plant cultivation seedling technologies, are concretely a kind of orchid mating systems.
Background technology
Orchid refers to the general name of entire orchid (Orchidaceae).In China, traditional " orchid " concept only refers to production
Orchid family, Cymbidium (Cymbidium) plant in China claim state blue, ground life, therefore also known as Terrestrial orchid.Orchid is a kind of ornamental
Value and the high rare species of economic value.
Orchid in Chinese tradition famous flower only refers to the several Terrestrial orchid being distributed in Cymbidium Plants, such as Chunlan, favour
Orchid, sword-leaved cymbidium, Chinese cymbidium and cold orchid etc., i.e. usually signified " China blue ".The tropical orchid of this kind of orchid and large flower and brilliant color is very much not
Identical, not eye-catching gorgeous state, not very large flower, leaf but have simple gentle and quiet, simple and elegant noble and unsullied makings, meet very much east
The aesthetical standard of people.There is the cultivation history in more than 1,000 years in China.
But the inadequate science of current Orchis planting, cause the poor quality of orchid, survival rate low.
Invention content
Present invention aims at a kind of orchid mating system is provided, the rooting rate and transplanting survival rate of orchid are improved.
The present invention includes following technical step：
（1）The culture of rootage of orchid：
From 50 days adventitious buds of subculture choose well-grown, the adventitious bud size of state consistency controls between 2-3cm, it is inoculated in MS trainings
It supports and is cultivated 30 days on base；After adventitious bud is inoculated on culture medium, orchid takes root；
（2）Regeneration plant hardening
The rooted seedling for growing 2~4 leaves can be subjected to hardening, bottle mouth sealing film is first opened and placed between tissue culture 2 weeks, then will
Rooted seedling is placed in together with container in transplanting facility, is placed 2 days；
（3）Transplantation of Regenerated Plantlets
First the 600-700 liquid of 60% carbendazol wettable powder of sphagna is carried out disinfection before transplanting, is then pinched and is held with hand, it is anhydrous
Lower drop can be used to transplant, and when transplanting carefully takes out rooted seedling, and the culture medium of root adherency is washed away with clear water, children is encased with sphagna
Seedling root is transplanted in hole tray.
Preferably, step（1）The MS culture mediums are：Ferric sulfate 20-25g/L, protein hydrolysate 5-10g/L, coconut milk
5-15ml/L, sucrose 15-20g/L, agar powder 1-5g/L, silk reeling wastewater 50-80ml/L, activated carbon 1-5g/L, Tomato juice 20-
30ml/L, tea waste residue 5-10g/L, perlite 5-10g/L and gibberellin 3-5g/L.
Preferably, step（1）The condition of culture is：Intensity of illumination is 2000~3000lux, light application time 10h/
D, cultivation temperature are 20~30 DEG C, humidity 85%.
Preferably, step（3）The condition of culture is：Intensity of illumination is 2000~3000lux, light application time 12h/
D, cultivation temperature are 20~30 DEG C, humidity 75%.
It is an advantage of the invention that：
（1）Orchid tissue-cultured seedling plant through the invention, taking root, fast, rooting rate is high；
（2）Orchid tissue-cultured seedling robust plant, the easy transplant survival taken root using this method；
（3）The orchid mating system of the present invention is easy to operate, at low cost, is suitable for the quick breeding of most ornamental orchids.
Specific implementation mode
Embodiment 1
The present invention includes following technical step：
（1）The culture of rootage of orchid：
From 50 days adventitious buds of subculture choose well-grown, the adventitious bud size of state consistency controls between 2-3cm, it is inoculated in MS trainings
It supports and is cultivated 30 days on base；After adventitious bud is inoculated on culture medium, orchid takes root；
（2）Regeneration plant hardening
The rooted seedling for growing 2~4 leaves can be subjected to hardening, bottle mouth sealing film is first opened and placed between tissue culture 2 weeks, then will
Rooted seedling is placed in together with container in transplanting facility, is placed 2 days；
（3）Transplantation of Regenerated Plantlets
First the 600-700 liquid of 60% carbendazol wettable powder of sphagna is carried out disinfection before transplanting, is then pinched and is held with hand, it is anhydrous
Lower drop can be used to transplant, and when transplanting carefully takes out rooted seedling, and the culture medium of root adherency is washed away with clear water, children is encased with sphagna
Seedling root is transplanted in hole tray.
Step（1）The MS culture mediums are：Ferric sulfate 25g/L, protein hydrolysate 5g/L, coconut milk 10ml/L, sucrose
15g/L, agar powder 2g/L, silk reeling wastewater 60ml/L, activated carbon 3g/L, Tomato juice 25ml/L, tea waste residue 8g/L, perlite
7g/L and gibberellin 3g/L.
Step（1）The condition of culture is：Intensity of illumination is 2500lux, light application time 10h/d, and cultivation temperature is
28 DEG C, humidity 85%.
The condition of culture is：Intensity of illumination is 2500lux, light application time 12h/d, and cultivation temperature is 28 DEG C, wet
Degree 75%.
Claims (4)
1. a kind of orchid mating system, including orchid takes root, hardening, transplanting, specifically comprise the steps of：
（1）The culture of rootage of orchid：
From 50 days adventitious buds of subculture choose well-grown, the adventitious bud size of state consistency controls between 2-3cm, it is inoculated in MS trainings
It supports and is cultivated 30 days on base；After adventitious bud is inoculated on culture medium, orchid takes root；
（2）Regeneration plant hardening
The rooted seedling for growing 2~4 leaves can be subjected to hardening, bottle mouth sealing film is first opened and placed between tissue culture 2 weeks, then will
Rooted seedling is placed in together with container in transplanting facility, is placed 2 days；
（3）Transplantation of Regenerated Plantlets
First the 600-700 liquid of 60% carbendazol wettable powder of sphagna is carried out disinfection before transplanting, is then pinched and is held with hand, it is anhydrous
Lower drop can be used to transplant, and when transplanting carefully takes out rooted seedling, and the culture medium of root adherency is washed away with clear water, children is encased with sphagna
Seedling root is transplanted in hole tray.
2. orchid mating system according to claim 1, which is characterized in that step（1）The MS culture mediums are：Sulfuric acid
Iron 20-25g/L, protein hydrolysate 5-10g/L, coconut milk 5-15ml/L, sucrose 15-20g/L, agar powder 1-5g/L, silk reeling wastewater
50-80ml/L, activated carbon 1-5g/L, Tomato juice 20-30ml/L, tea waste residue 5-10g/L, perlite 5-10g/L and red mould
Plain 3-5g/L.
3. orchid mating system according to claim 1, which is characterized in that step（1）The condition of culture is：Illumination
Intensity is 2000~3000lux, light application time 10h/d, and cultivation temperature is 20~30 DEG C, humidity 85%.
4. orchid mating system according to claim 1, which is characterized in that step（3）The condition of culture is：Illumination
Intensity is 2000~3000lux, light application time 12h/d, and cultivation temperature is 20~30 DEG C, humidity 75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711110503.4A CN108353785A (en) | 2017-11-12 | 2017-11-12 | A kind of orchid mating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711110503.4A CN108353785A (en) | 2017-11-12 | 2017-11-12 | A kind of orchid mating system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108353785A true CN108353785A (en) | 2018-08-03 |
Family
ID=63009890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711110503.4A Pending CN108353785A (en) | 2017-11-12 | 2017-11-12 | A kind of orchid mating system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108353785A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613080A (en) * | 2012-03-31 | 2012-08-01 | 常熟市佳盛农业科技发展有限公司 | Method for rapidly propagating wild ginseng |
| CN102630447A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Rapid propagation method of Taiwan mustard green |
| CN103181325A (en) * | 2013-04-01 | 2013-07-03 | 南京林业大学 | Tissue culture rapid propagation system of European hornbeam |
| CN105993954A (en) * | 2016-05-31 | 2016-10-12 | 徐振飞 | Phalaenopsis spp. rooting and seedling strengthening method |
-
2017
- 2017-11-12 CN CN201711110503.4A patent/CN108353785A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630447A (en) * | 2012-03-28 | 2012-08-15 | 常熟市滨江农业科技有限公司 | Rapid propagation method of Taiwan mustard green |
| CN102613080A (en) * | 2012-03-31 | 2012-08-01 | 常熟市佳盛农业科技发展有限公司 | Method for rapidly propagating wild ginseng |
| CN103181325A (en) * | 2013-04-01 | 2013-07-03 | 南京林业大学 | Tissue culture rapid propagation system of European hornbeam |
| CN105993954A (en) * | 2016-05-31 | 2016-10-12 | 徐振飞 | Phalaenopsis spp. rooting and seedling strengthening method |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180803 |
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