CN108344821A - The liquid phase Tandem Mass Spectrometry Analysis method of vitamin A in biological sample - Google Patents
The liquid phase Tandem Mass Spectrometry Analysis method of vitamin A in biological sample Download PDFInfo
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- CN108344821A CN108344821A CN201710061197.3A CN201710061197A CN108344821A CN 108344821 A CN108344821 A CN 108344821A CN 201710061197 A CN201710061197 A CN 201710061197A CN 108344821 A CN108344821 A CN 108344821A
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- G—PHYSICS
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Abstract
The present invention relates to the liquid phase Tandem Mass Spectrometry Analysis methods of vitamin A in biological sample, including step：A kind of sample to be tested, precipitating reagent and extractant are provided；Sample to be tested and precipitating reagent are mixed, the protein in sample to be tested is made to precipitate；To form the first solid-liquid mixed liquor；Extraction processing is carried out with extractant pair the first solid-liquid mixed liquor, obtains the extraction phase containing vitamin A；Dry extraction phase, obtains dry vitamin A sample；The vitamin A sample is redissolved using organic solvent, and carries out liquid phase tandem mass spectrum detection；The analysis result of vitamin A in Organism Samples is obtained based on standard curve.The analysis method of the present invention can be applied to the quantitative detection of vitamin A in the human serum samples of clinical acquisitions, to determine that changes of contents provides strong support to the compound with physiological function, the relationship of disease in vivo.
Description
Technical field
The invention belongs to chemical analysis detection fields, in particular it relates to which the purifying of vitamin A carries in biological sample
The method for taking and being detected using liquid phase tandem mass spectrum, method of the invention have the characteristics that quick, efficient, sensitive, are true
Changes of contents lays the foundation the fixed compound with physiological function, the relationship of disease in vivo.
Background technology
Vitamin A, also known as retinol and antixerophthalmic vitamin, as the term suggests it is to have the function of that human body maintains normal vision
Indispensable nutrient.In addition to this, vitamin A can safeguard the health of Epithelial cell and promote immunoglobulin
Synthesis maintains bone normal growth and development, growth is promoted to inhibit tumour growth with reproduction, is that humans and animals maintain body normally to give birth to
Reason function is kept fit necessary micro-content organism, is played an important role in growth in humans, metabolism, growth course.Dimension
Raw element A is usually obtained from food, and internal reserves are seldom.But when there is certain physiology, pathological change in human body, vitamin A
Intake can be reduced or consumption increases, so as to cause hypovitaminosis, conversely, certain physiological changes can cause vitamin to exist
Accumulation in vivo, therefore clinically vitamin A content deviation normal value shows as vitamin-deficiency or vitaminosis in human body.
In order to monitor the changes of contents of vitamin A in human body, people establish numerous detection methods, such as high-efficient liquid phase color according to its characteristic
Spectrometry (Petr, S., Dalibor, S., Martin, O., Radek, S., Petr, S.Cur.Anal.Chem.2009,5 (4),
311-315), Capillary Electrophoresis fluorescent exciting spectrometry (Ma, Y., Wu, Z., Furr, H.C., Lammi-Keefe, C., Craft,
N.E.J.Chrom.B:Biomed.Appl.1993,616 (1), 31-37.) and chemoluminescence method (Asgher, M., Yaqoob,
M., Waseem, A., Nabi, A.Luminescence 2011,26 (6), 416-423) etc., these methods are in detecting nutriment
Vitamin A active ingredient, and research Vitamin A Metabolism access process suffer from many applications.But in clinical examination process
In, due to human sample source complexity, it is big to be disturbed factor, and simple spectroscopic methodology is easy to be done by sample ingredient color itself
Disturb, often provide false positive or false negative as a result, it is difficult to realize in clinical application quickly, it is accurate, specifically detect
Vitamin A.
Therefore, there is an urgent need in the art to a kind of analysis methods quick and precisely detecting vitamin A compounds.In order to meet matter
Spectrum detection, avoids the impurity contaminated equipment in sample, needs to carry out pre-treatment to human sample, the present invention provides a kind of optimizations
Biological sample pre-treating method realized to vitamin A in clinical biochemical sample in conjunction with liquid phase tandem mass spectrum detection method
Assay.
Invention content
The purpose of the present invention is to provide a kind of methods of the detection vitamin A of fast accurate.
The first aspect of the present invention provides a kind of analysis to the vitamin A progress liquid phase tandem mass spectrum in sample to be tested
Method, including step：
(1) a kind of sample to be tested of offer, precipitating reagent and extractant；
(2) sample to be tested and precipitating reagent are mixed, the protein in sample to be tested is made to precipitate；To form the
One solid-liquid mixed liquor；
(3) extraction processing is carried out with extractant pair the first solid-liquid mixed liquor, obtains the extraction phase containing vitamin A；
(4) dry extraction phase, obtains dry vitamin A sample；With
(5) it uses organic solvent to redissolve the vitamin A sample, and carries out liquid phase tandem mass spectrum detection；With
(6) analysis result of vitamin A in Organism Samples is obtained based on standard curve.
In another preferred example, further include that centrifugal treating is carried out to the first solid-liquid mixed liquor in the step (3).
In another preferred example, the centrifugation of centrifugal treating is carried out in the step (3) to the first solid-liquid mixed liquor
Power is 10000~15000g.
In another preferred example, the time centrifuged to the first solid-liquid mixed liquor in the step (3) is 3~
8min。
In another preferred example, the drying means in the step (4) purges for nitrogen.
In another preferred example, the precipitating reagent is selected from the group：Acetonitrile, methanol, or combinations thereof.
In another preferred example, the precipitating reagent is acetonitrile.
In another preferred example, the sample to be tested is serum or blood plasma.
In another preferred example, the sample to be tested is human serum or blood plasma.
In another preferred example, the volume of the precipitating reagent is 2~4 times of organism sample volume, preferably, being
2.5~3.5 times, be 3 times more preferably.
In another preferred example, the extractant is the alkane for including 4~8 carbon atoms, preferably, being hexane, more
Goodly, it is n-hexane.
In another preferred example, the vitamin A sample is redissolved using organic solvent selected from the group below：Methanol, acetonitrile,
Or combinations thereof；Preferably, being methanol.
In another preferred example, the analysis method further includes the drafting of standard curve, including steps are as follows：
(i) standard solution, precipitating reagent and extractant are provided, the standard solution contains the dimension life of known concentration
The vitamin A Isotopic Internal Standard of plain A standard items and known concentration；
(ii) standard solution and precipitating reagent are mixed, the protein in standard solution is made to precipitate；To
Form the second solid-liquid mixed liquor；
(iii) extraction processing is carried out with extractant pair the second solid-liquid mixed liquor, obtains the extraction containing vitamin A standard items
Phase；
(iv) dry extraction phase, obtains dry vitamin A standard items；With
(v) it uses organic solvent to redissolve the vitamin A standard items, and carries out liquid phase tandem mass spectrum detection, to obtain
Obtain standard curve.
In another preferred example, the standard solution is human serum albumin solution
In another preferred example, the precipitating reagent in the step (ii) is acetonitrile.
In another preferred example, the extractant in the step (iii) is n-hexane.
In another preferred example, the organic solvent in the step (v) is methanol.
In another preferred example, the vitamin A standard items are at least 2 groups, and the dimension containing known various concentration
Raw element A.
In another preferred example, the vitamin A standard concentration is selected from the group：0,0.05,0.1,0.2,0.5,1,
2.5, or 5 μ g/mL.
In another preferred example, the standard solution contains human serum albumins and physiological saline.
In another preferred example, a concentration of 30~50mg/mL of human serum albumins in the standard solution.
In another preferred example, in liquid phase tandem mass spectrum detection, using APCI ion sources.
In another preferred example, in liquid phase tandem mass spectrum detection, using acetonitrile and water mixed liquid as mobile phase.
In another preferred example, in liquid phase tandem mass spectrum detection, acquisition time 3min.
In another preferred example, in liquid phase tandem mass spectrum detection, using cation scanning, multiple-reaction monitoring mould
Formula.
In another preferred example, in liquid phase tandem mass spectrum detection, while multiple ion channels being scanned.
It should be understood that within the scope of the present invention, above-mentioned each technical characteristic of the invention and have in below (eg embodiment)
It can be combined with each other between each technical characteristic of body description, to form a new or preferred technical solution.As space is limited, exist
This no longer tires out one by one states.
Description of the drawings
Vitamin A content analyzes key step schematic diagram in Fig. 1 embodiments 1.
Fig. 2 vitamin A standard curve examples.
The influence of lipid signaling when Fig. 3 different solvents are as extractant.
Specific implementation mode
The present inventor is had unexpectedly discovered a kind of efficiently quickly in biological sample for the first time by depth studying extensively
The liquid phase Tandem Mass Spectrometry Analysis method of vitamin A.The analysis method of the present invention is using acetonitrile as albumen in serum biological sample
The precipitating reagent of matter, the extractant using n-hexane as vitamin A.Due to the poor compatibility of acetonitrile and n-hexane so that more
Efficiently extract vitamin A.On this basis, the present invention is completed.
The analysis method of the present invention
It, can be with the present invention provides a kind of method quantitatively detecting vitamin A in biological sample based on liquid phase tandem mass spectrum
Analysis detection for vitamin A compounds in serum or plasma sample.The method includes the following steps：
A) in biological sample the release of vitamin A and protein removing；
B) vitamin A in biological sample is selectively extracted using liquid-liquid extraction；
C) solvent drying after extraction is redissolved and quantitatively detects vitamin A for LC-MS/MS.
LC-MS/MS analyzes optimum condition used：Liquid phase is used as organic phase, water using acetonitrile (containing 0.1% formic acid)
(0.1% formic acid) is used as water phase, total acquisition time 3min to substantially increase detection flux；Mass spectrum uses APCI ion sources, effectively
Ground reduces matrix effect of the human serum for vitamin A compounds, acquires in the positive-ion mode, same using multiple-reaction monitoring
When scanning target compound vitamin A and its Isotopic Internal Standard (vitamin A-d5), according to Isotopic Internal Standard and determinand ion
Change the similar characteristic of efficiency, by the concentration relationship of determinand the two corresponding with interior target peak area ratio, calculates testing concentration.By
In vitamin A be endogenous compound, therefore the present invention using human serum albumins be used as bare substrate, pass through addition known gradient
A series of vitamin A standard solution of concentration, obtain standard curve, opposite according to the vitamin A and its interior target that collect
Peak area substitutes into standard curve linear equation, and the concentration value of vitamin A in biological sample is calculated.Computational methods are as follows：It presses
The vitamin A concentration in human serum is calculated according to the detected standard curve in (1)~(4) (such as y=ax+b),CSampleFor vitamin A content in serum sample, y is vitamin A compounds relative to its isotope
Interior target peak area ratio, b are linear equation intercept, and a is linear equation slope.
There is following advantage in the analysis detection of present invention vitamin A compounds in serum or plasma sample：First,
Present invention employs the acetonitrile precipitation albumen of 3 times of sample volumes, protein precipitation effect is better than methanol, is more advantageous to vitamin A
Release, and acetonitrile and the dissolubility of follow-up liquid-liquid extraction are poor, are more advantageous to and selectively extract vitamin A.
Secondly, in liquid-liquid extraction step, using n-hexane as Extraction solvent, can effectively with protein precipitation solvent and
Biological sample system layering, vitamin A are liposoluble vitamin, are selectively allocated in the organic phase of n-hexane, and sample is excluded
Interference of other impurities, more effectively eliminates the salt and albumen of the sample middle and high concentration for being unfavorable for Mass Spectrometer Method in this.
Under liquid-phase condition, by optimization, when acetonitrile is as mobile phase, separating effect is better than methanol, eliminates phosphatide
Interference, therefore, mobile phase elution time foreshortens to 3min, can achieve the purpose that high-throughput detection.
In Mass Spectrometer Method, although under the conditions of pure solvent, the signal of APCI ion sources is weaker than traditional ESI source signals,
But due to the matrix interference in human blood sample sheet, in the sources ESI, the signal of the vitamin A in sample is significantly lower than in dicyandiamide solution
Signal, show apparent matrix effect.And present invention employs APCI ion sources due to by sample substance interference because
Element is weakened, and the matrix effect of biological sample can be eliminated, more conducively to the accurate quantitative analysis of biological sample.
The specific implementation step of the analysis method of the present invention
The method of the present invention is carried out by step in detail below, includes the drafting (step (1)~(4)) and life of standard curve
The detection (step (5)~(8)) of vitamin A concentration in object sample：
(1) human serum of the vitamin A of standard items containing known concentration (0,0.05,0.1,0.2,0.5,1,2.5,5 μ g/mL) is taken
300 μ L (3 times of sample volumes) acetonitrile is added (containing dimension in 100 μ L of albumin solution (normal saline solution of 40mg/mL albumen)
Raw 0.5 μ g/mL of element A Isotopic Internal Standards), it is used for protein precipitation, centrifuges 13000g*5min；
(2) 1mL n-hexanes are added in sample after the albumen precipitation that (1) obtains, is used for liquid-liquid extraction, fully vibrates 1min
Two are made to be in contact uniformly, fat-soluble stronger vitamin A compounds, which enter, to be preferentially entered in organic phase n-hexane, is centrifuged
13000g*5min contributes to water phase and organic phase to be layered；
(3) in the two-phase of the centrifugation layering obtained from (2), 900 μ L of upper organic phase are drawn in 96 orifice plates, and in room temperature
It is lower to be dried up with mild nitrogen, it obtains dry sample and invests bottom of bottle；
(4) the drying sample that (3) obtain is redissolved with 100 μ L methanol solutions, 700rpm fully shakes up 10min, 10 μ L of sample introduction
Liquid phase tandem mass spectrum detects, and obtains standard curve；
(5) 100 μ L of human serum sample are taken, 300 μ L acetonitriles (0.5 μ g/mL of Isotopic Internal Standard containing vitamin A) are added, are used for
Protein precipitation centrifuges 13000g*5min；
(6) 1mL n-hexanes are added in sample after the albumen precipitation that (5) obtain, are used for liquid-liquid extraction, fully oscillation makes two
It is in contact uniformly, fat-soluble stronger vitamin A compounds, which enter, to be preferentially entered in organic phase n-hexane, and 13000g* is centrifuged
5min contributes to water phase and organic phase to be layered；
(7) in the two-phase of the centrifugation layering obtained from (6), 900 μ L of upper organic phase is drawn, is used in combination mild nitrogen to blow, obtains
Dry sample invests bottom of bottle；
(8) the drying sample that (7) obtain is redissolved with 100 μ L methanol solutions, liquid phase tandem mass spectrum detection is carried out, according to (1)
The detected standard curve in~(4) (such as y=ax+b) calculates the vitamin A concentration in human serum,CSampleFor vitamin A content in serum sample, y is vitamin A compounds relative to its isotope
Interior target peak area ratio, b are linear equation intercept, and a is linear equation slope.
Main advantages of the present invention
(1) standard curve that analysis method of the invention obtains has good linear, wherein coefficient R2Value reaches
0.9999, therefore, this analysis method more can accurately measure vitamin A content.
(2) analysis method step of the invention is few, and easy to operate, agents useful for same is cheap and easy to get, therefore, highly promotes
Using.
Using
The analysis method of the present invention is applied to the quantitative detection of vitamin A in biological sample such as serum or plasma sample,
Be it is a kind of quickly, high-throughput, sensitive detection method, can be applied to determining for vitamin A in the human serum sample of clinical acquisitions
Amount detection, to determine that changes of contents provides strong support to the compound with physiological function, the relationship of disease in vivo.
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention
Rather than it limits the scope of the invention.In the following examples, the experimental methods for specific conditions are not specified, usually according to conventional strip
Part, or according to the normal condition proposed by manufacturer.Unless otherwise stated, otherwise percentage and number are calculated by weight.
Embodiment 1：The analysis detection of vitamin A in human serum sample
The 100 μ L of human serum albumin solution for taking the vitamin A containing known concentration respectively containing vitamin A concentration are respectively 0,
0.05,300 μ L acetonitrile precipitation albumen are added in 0.1,0.2,0.5,1,2.5,5 μ g/mL, centrifuge 13000rpm*5min.It adds
1mL n-hexanes, oscillation mixing extraction, centrifuge 13000rpm*5min, take 900 μ L of upper organic phase in 96 orifice plates, mild nitrogen
Drying is redissolved with 100 μ L methanol, is used for Mass Spectrometer Method, and obtain standard curve under human serum albumins matrix condition.
It takes 100 μ L of serum sample in 1.5mLEP pipes, 300 μ L acetonitrile precipitation albumen is added, centrifuge 13000rpm*5min.
1mL n-hexanes are added, oscillation mixing extraction centrifuges 13000rpm*5min, takes 900 μ L of upper organic phase in 96 orifice plates, temperature
It dries up with nitrogen, is redissolved with 100 μ L methanol, be used for Mass Spectrometer Method.APCI ion sources are used in the detection of liquid phase tandem mass spectrum.Fig. 1
For the key step of the content analysis of vitamin A in the present embodiment 1.In method of the present invention, serum sample used
Or the dosage of solvent is not limited to this.
Standard curve is as shown in Figure 2 under gained human serum albumins matrix condition.According to linear equation y=0.927x+
0.0175,Y is peak area ratio of the vitamin A compounds relative to its Isotopic Internal Standard, people
It is 0.33 μ g/mL that vitamin A concentration is measured in serum sample.
Table 1 is the matrix effect under APCI ion source testing conditions：
Table 1
| Vitamin A internal standard peak area | RSD% | |
| Bare substrate | 2.52E+05 | 2.44 |
| Human serum | 2.32E+05 | 3.29 |
As can be seen from the table, it is close to be inside marked on signal strength in bare substrate and human serum, no matrix effect.
Comparative example 1：
Using the method as described in implementing 1, the difference is that, acetonitrile is replaced in human serum albumin solution with methanol
Albumen carry out precipitation process.
Table 2 is the vitamin A peak area obtained when making precipitation process to albumen with methanol and acetonitrile respectively and opposite
Standard deviation：
Table 2
| Vitamin A peak area | RSD% | |
| Methanol extraction | 2.52E+04 | 3.19 |
| Acetonitrile precipitation | 4.05E+04 | 4.59 |
From Table 2, it can be seen that acetonitrile precipitation albumen is more preferable to the extraction effect of target compound, the vitamin obtained
A contents are extracted more than methanol.
Comparative example 2：
Using the method as described in implementing 1, the difference is that, use ethyl acetate and methyl tertiary butyl ether(MTBE) as extraction respectively
Agent is taken, the vitamin A in sample is extracted.
From figure 3, it can be seen that can effectively exclude in blood sample phosphatide for targeted using n-hexane extraction
The interference of analyte detection is closed, and ethyl acetate and methyl tertiary butyl ether(MTBE) can all extract the phosphatide in blood sample, lipid signaling is very strong, easily
It generates signal to inhibit, influences the signal of determinand.
Comparative example 3：
Using the method as described in implementing 1, the difference is that, replace APCI ion sources to be monitored with ESI ion sources.
Table 3 is the matrix effect under ESI ion source testing conditions：
Table 3
| Vitamin A internal standard peak area | RSD% | |
| Water | 3.44E+05 | 3.26 |
| Bare substrate | 2.55E+05 | 2.89 |
| Human serum | 2.95E+04 | 10.37 |
From table 3 it is observed that the Isotopic Internal Standard of target compound is in human serum and ratioing signal phase in bare substrate
Poor 10 times.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To be made various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (10)
1. a kind of vitamin A in sample to be tested carries out the analysis method of liquid phase tandem mass spectrum, which is characterized in that including step
Suddenly：
(1) a kind of sample to be tested of offer, precipitating reagent and extractant；
(2) sample to be tested and precipitating reagent are mixed, the protein in sample to be tested is made to precipitate；It is solid to form first
Liquid mixed liquor；
(3) extraction processing is carried out with extractant pair the first solid-liquid mixed liquor, obtains the extraction phase containing vitamin A；
(4) dry extraction phase, obtains dry vitamin A sample；With
(5) it uses organic solvent to redissolve the vitamin A sample, and carries out liquid phase tandem mass spectrum detection；With
(6) analysis result of vitamin A in sample to be tested is obtained based on standard curve.
2. analysis method as described in claim 1, which is characterized in that the precipitating reagent is selected from the group：Acetonitrile, methanol,
Or combinations thereof.
3. analysis method as described in claim 1, which is characterized in that the sample to be tested is serum or blood plasma.
4. analysis method as described in claim 1, which is characterized in that the volume of the precipitating reagent is sample to be tested volume
2~4 times, preferably, be 2.5~3.5 times, more preferably, be 3 times.
5. analysis method as described in claim 1, which is characterized in that the extractant is comprising 4~8 carbon atoms
Alkane is n-hexane more preferably preferably, being hexane.
6. analysis method as described in claim 1, which is characterized in that described using organic solvent selected from the group below redissolution
Vitamin A sample：Methanol, acetonitrile, or combinations thereof；Preferably, being methanol.
7. analysis method as described in claim 1, which is characterized in that the analysis method further includes painting for standard curve
System, including steps are as follows：
(i) standard solution, precipitating reagent and extractant, the standard solution are provided and contains the vitamin A mark of known concentration
The vitamin A Isotopic Internal Standard of quasi- product and known concentration；
(i i) mixes standard solution and precipitating reagent, and the protein in standard solution is made to precipitate；To be formed
Second solid-liquid mixed liquor；
(iii) extraction processing is carried out with extractant pair the second solid-liquid mixed liquor, obtains the extraction phase containing vitamin A standard items；
(iv) dry extraction phase, obtains dry vitamin A standard items；With
(v) it uses organic solvent to redissolve the vitamin A standard items, and carries out liquid phase tandem mass spectrum detection, to be marked
Directrix curve.
8. analysis method as described in claim 7, which is characterized in that the standard solution is at least 2 groups, and contains
The vitamin A standard items of known various concentration.
9. analysis method as described in claim 7, which is characterized in that the standard solution contains human serum albumins
And physiological saline.
10. analysis method as described in claim 1, which is characterized in that in the liquid phase tandem mass spectrum detection, use
APCI ion sources.
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| CN113759039A (en) * | 2021-08-26 | 2021-12-07 | 杨艳玲 | Synchronous detection method and device for beta-carotene and vitamin A |
| CN115166118A (en) * | 2022-08-08 | 2022-10-11 | 江苏格诺生物科技有限公司 | Pretreatment kit for detecting multiple vitamins in human serum and use method thereof |
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