CN108342341B - Vibrio strain for treating high-nitrogen low-carbon salt-containing wastewater and application thereof - Google Patents
Vibrio strain for treating high-nitrogen low-carbon salt-containing wastewater and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of sewage treatment, and particularly discloses a Vibrio strain (Vibrio sp.) MCW152 for treating high-nitrogen low-carbon salt-containing wastewater, which is preserved in China center for type culture Collection with the preservation numbers as follows: CCTCC No. m 2018030. The strain disclosed by the invention can effectively treat high-nitrogen low-carbon (C/N is less than or equal to 1) salt-containing wastewater, the nitrate nitrogen of the effluent of the system is less than 0.05mg/L, and the removal rate is more than 99%; the total nitrogen is less than 10mg/L, and the removal rate is 86.4 percent.
Description
Technical Field
The invention relates to a microbial strain, belongs to the technical field of sewage treatment, and particularly relates to a vibrio strain for treating high-nitrogen low-carbon salt-containing wastewater and application thereof.
Background
The treatment of wastewater containing nitrogen (ammonia nitrogen, nitrite nitrogen and nitrate nitrogen) is one of the important subjects in the field of wastewater treatment. In the control of nitrogen pollutants, biological denitrification technology is mainly adopted at home and abroad, and is the most widely applied sewage denitrification technology at present. The basic principle of biological denitrification is a process for converting organic nitrogen in sewage into nitrogen under the action of microorganisms, and the process comprises two reaction processes of nitrification and denitrification. The nitration reaction is completed by a group of autotrophic aerobic microorganisms, and is specifically completed by two stages of nitrite bacteria and nitrate bacteria respectively. The first step is to remove NH from nitrite bacteria4 +Oxidation to NO2 -The second step is to react NO with nitrobacteria2 -Further oxidation to NO3 -. The denitrification reaction is completed by a group of heterotrophic and anaerobic microorganisms, and NO is converted by denitrifying bacteria under the condition of NO oxygen or low oxygen2 -And NO3 -Reduction to nitrogen. It can be seen that there is a contradiction in the biological denitrification process itself: the nitration reaction needs longer sludge age and aerobic conditions, and the loss of nitrifying bacteria can be caused by the existence of a large amount of organic matters; the denitrifying bacteria need shorter sludge age and anoxic conditions, and are highly dependent on organic matters to provide electron donors for the denitrification process. The sewage denitrification technology based on the theory is complicated in process and high in energy consumption due to the difference of physiological mechanisms of nitrobacteria and denitrifying bacteria.
In addition, especially for high-nitrogen and low-carbon source wastewater (C/N is less than or equal to 1), the problems of insufficient carbon source, low total nitrogen removal rate and the like in the denitrification process are urgently needed to be solved, so that a feasible way is provided for efficient biological denitrification of the high-nitrogen wastewater. At present, the sources of high-nitrogen low-carbon wastewater are wide, and mainly comprise: the method is characterized in that sewage (circulating cooling water) is circularly discharged in the power industry, aquaculture wastewater (aquaculture, poultry farming, pig farming and the like), chemical wastewater (coking wastewater, fertilizer wastewater and the like), food processing wastewater (monosodium glutamate wastewater, pickle factory wastewater and the like), landfill leachate and the like are taken as aquaculture wastewater, in a high-density aquaculture system, about 75-80% of nitrogen in feed can enter aquaculture water, and is accumulated in different degrees in the form of ammonia nitrogen, nitrite nitrogen and especially high nitrate nitrogen to cause damage to aquaculture animals, so that the unit yield of seawater aquaculture is limited, and the discharged wastewater also contains rich nitrogen-containing compounds, can accelerate seawater eutrophication, cause red tide disasters and bring damage to the offshore ecological environment. Therefore, the method for rapidly eliminating the organic pollution in the culture environment is researched to recover and optimize the culture environment as soon as possible, and the method has important theoretical and practical significance for the healthy development of the marine culture industry in China and the sustainable utilization of mudflat and shallow sea resources.
The Chinese patent application, application publication Nos. CN106434422A, CN106434423A and CN106434424A, all disclose a vibrio with capability of denitrifying seawater, which mainly uses organic matters in water as a carbon source, but when the wastewater treatment method is used for circulating mariculture wastewater treatment, the wastewater treatment method is influenced by the particularity of seawater and the culture wastewater, such as high salt content, high nitrogen and low carbon (especially high nitrate nitrogen), and the carbon source needs to be added, so that the treatment effect is difficult to achieve the purpose of high efficiency, and meanwhile, if a liquid carbon source is added, the method also has excessive risk, and puts high requirements on the stable operation and maintenance of the system. Taking aquaculture wastewater as an example, NO is generated in a circulating water culture system3 -In the case of fluctuations in-N, it is more difficult to control the amount of carbon source to be added.
Disclosure of Invention
In order to solve the technical problems, the invention discloses a vibrio strain for treating high-nitrogen low-carbon salt-containing wastewater and an application thereof, wherein the vibrio strain is used for improving the denitrification effect by fixing salt-tolerant dirt-removing bacteria with a high-efficiency denitrification function in a solid biological growth promoter.
In order to achieve the purpose, the invention discloses a Vibrio (Vibrio sp.) strain MCW152 which is preserved in China center for type culture Collection with the preservation number as follows: CCTCC No. m 2018030; the preservation date is as follows: 2018-01-15, and the address of a preservation unit is as follows: wuhan university school of eight-channel 299 # in Wuchang area of Wuhan city, Hubei province.
The invention also discloses application of the Vibrio (Vibrio sp.) strain MCW152 in treatment of high-nitrogen low-carbon salt-containing wastewater.
As the optimization of the technical scheme of the invention, the invention also discloses a method for treating the high-nitrogen low-carbon salt-containing wastewater, which comprises the following steps:
1) loading a biodegradable polymer into a wastewater treatment container, and inoculating Vibrio (Vibrio sp.) strain MCW152 onto the biodegradable polymer;
2) placing the wastewater treatment container in high-nitrogen low-carbon salt-containing wastewater which circularly flows;
3) respectively measuring the total nitrogen amount of the high-nitrogen low-carbon salt-containing wastewater at the water inlet and the total nitrogen amount of the high-nitrogen low-carbon salt-containing wastewater after being treated at the water outlet.
Further preferably, in the step 1), the biodegradable polymer is loaded into a wastewater treatment container, and after the biodegradable polymer is added, the wastewater is kept still for 3 to 7 days, and then the Vibrio (Vibrio sp) strain MCW152 bacterial liquid is inoculated.
Still further preferably, in the step 1), the volume of the biodegradable polymer is 20 to 50% of the volume of the wastewater treatment vessel.
More preferably, the volume of the Vibrio (Vibrio sp.) strain MCW152 is 0.1 to 1 per mill of the volume of the wastewater.
More preferably, in the step 1), the biodegradable polymer is a polyester-based molecule having a diameter of 3-5 mm, the polyester-based molecule is an off-white spherical particle, and has stable physical and chemical properties, and the polyester-based molecule is used as one of carbon sources of Vibrio (Vibrio sp) strain MCW 152.
Still more preferably, in the step 2), the carbon-nitrogen ratio of the high-nitrogen low-carbon salt-containing wastewater is less than or equal to 1.
The invention also discloses a microbial agent for treating the high-nitrogen low-carbon salt-containing wastewater, the active component of the microbial agent is the Vibrio (Vibrio sp.) strain MCW152, and the microbial agent is mainly used for treating the wastewater of mariculture. The microbial agent is prepared by adsorbing and drying the vibrio strains and the sterile attachments.
As the optimization of the technical scheme of the invention, the pH value of the high-nitrogen low-carbon salt-containing wastewater is 7.
As the optimization of the technical scheme of the invention, the water temperature of the high-nitrogen low-carbon salt-containing wastewater is 30 ℃.
Has the advantages that:
1. the Vibrio (Vibrio sp.) strain MCW152 is used as an aerobic denitrification high-efficiency denitrification microorganism, and can achieve the purpose of efficiently removing nitrogen from high-nitrogen low-carbon salt-containing wastewater;
2. according to the method for treating the high-nitrogen low-carbon salt-containing wastewater, the problem of insufficient carbon source in the biological treatment technology of the high-nitrogen low-carbon wastewater is effectively solved by fixing the aerobic denitrification efficient denitrifying bacteria on the biodegradable polymers (BDPs) and optimizing the process parameters;
3. the biodegradable polymers (BDPs) used in the method for treating the high-nitrogen low-carbon salt-containing wastewater DO not leach harmful substances into a water body, have small influence on the quality of effluent water, have strong adaptability to pH and DO impact loads, and the Vibrio (Vibrio sp.) strain MCW152 fixed on the biodegradable polymers has stable and efficient denitrification activity;
4. the method for treating the high-nitrogen low-carbon salt-containing wastewater has the advantages of reducing energy consumption, saving carbon sources, reducing sludge generation amount, reducing the volume of a reactor and the like, and can realize effective treatment on the high-nitrogen low-carbon (C/N is less than or equal to 1) salt-containing wastewater, wherein nitrate nitrogen in effluent of a system is less than 0.05mg/L, and the removal rate is more than 99%; the total nitrogen is less than 10mg/L, and the removal rate is 86.4 percent.
Drawings
FIG. 1 is a transmission electron micrograph of Vibrio (Vibrio sp.) strain MCW152 of the present invention;
FIG. 2 shows the metabolism of NO by Vibrio (Vibrio sp.) strain MCW152 of the present invention3 --N optimum carbon source curve;
FIG. 3 shows the metabolism of NO by Vibrio (Vibrio sp.) strain MCW152 of the present invention2 --N optimum carbon source curve;
FIG. 4 is a temperature profile of the most suitable strain of Vibrio (Vibrio sp.) strain MCW152 of the present invention for its denitrification capability;
FIG. 5 is a pH optimum curve showing denitrification capability of Vibrio (Vibrio sp.) strain MCW152 of the present invention;
FIG. 6 is a curve showing the denitrification performance of Vibrio (Vibrio sp.) strain MCW152 according to the present invention;
FIG. 7 is a scanning electron micrograph of a biodegradable polymer in a wastewater treatment system according to an embodiment of the present invention before use;
FIG. 8 is a scanning electron micrograph of a biodegradable polymer in a wastewater treatment system according to an embodiment of the present invention after use;
FIG. 9 is a graph showing denitrification effects obtained by the operation of the wastewater treatment system according to the embodiment of the present invention after being left idle for 3 weeks.
In order to better explain the invention, the following further illustrate the main content of the invention in connection with specific examples, but the content of the invention is not limited to the following examples. It should be noted that reference may be made to screening methods, buffer formulations, and common media formulations, etc., as described in examples of the present invention, in Zhao and Han Jiang, eds "microbiology laboratory". Other various experimental operations involved in the present invention are conventional in the art and are not specifically described herein, and those skilled in the art can refer to various general tool books, scientific documents, or related specifications, manuals, etc. before the filing date of the present application.
Detailed Description
Example 1
Isolation and identification of MCW152 Strain:
selecting sediment from coastal seawater farms in Tianjin city as a screening mud sample, wherein the specific screening scheme is as follows:
1) enrichment culture of the strain: dissolving a newly-taken screening mud sample by using sterilized distilled water, exposing the mud sample for 24 hours in the air, standing, taking a supernatant, inoculating the supernatant into an enrichment medium (FM), carrying out shake cultivation at 30 ℃ and 160rpm for about 3 days, inoculating the culture solution into a fresh enrichment medium (FM) according to the inoculation amount of 1% after the culture solution becomes turbid, continuing the cultivation, and repeating the steps for 3-5 times to obtain a purified mixed flora;
the enrichment medium (FM) in step 1) above comprises the following main components: peptone 8.6g, NaCl 6.4g, KNO31.0g, 1.5g sodium succinate, and pH 7.0-7.5, wherein each 1000mL seawater is used.
2) Separating and purifying strains: adding 1mL of the enriched bacterial liquid obtained in the step 1) into 9mL of sterile water, and uniformly mixing to obtain 10-1The diluted solution was 1mL10-1The diluted solution is added into 9mL of sterile water and mixed evenly to obtain 10-2Diluting the solution by analogy in sequence, and continuously diluting to obtain 10-3、10-4、10-5、10-6、10-7A series of dilutions; get 10-5、10-6、10-7Three gradients of dilution 100 μ L spread on solid medium plates (BTB) and incubated overnight at 30 ℃ until a single strain was obtained;
3) and (3) continuously culturing the strain: continuously inoculating the single strain obtained in the step 2) on a beef extract peptone agar slant culture medium, culturing for 24h, and then storing in a refrigerator at 4 ℃ for later use.
According to the appearance (color, diameter, transparency, edge uniformity, whether the edge is protruded or not, whether the surface is smooth or not and the like) of the strain, recording the colony morphology, inoculating the strain on a beef extract peptone agar slant culture medium, culturing for 24h, and storing in a refrigerator at 4 ℃ for later use to obtain the strain.
The solid medium plate (BTB) in the step 2) comprises the following main components: na (Na)2HPO4 7.9 g，KH2PO41.5g，MgSO4·7H20.1g of O, 2ml of trace element solution, KNO31.0g, 9.4g of sodium succinate, 1mL of BTB (0.1 g of bromothymol blue is dissolved in 10mL of absolute ethyl alcohol), pH 7.0 and 1000mL of seawater, wherein 15g/L of agar powder is also added into a solid culture medium, and the components of the microelement solution are as follows: EDTA 50.0g, ZnSO4 2.2g，CaCl2 5.5g，MnCl2·4H2O 5.06 g，FeSO4·7H2O 5.0g，(NH4)6Mo7O2·4H2O 1.1g，CuSO4·5H2O 1.57g， CoCl2·6H2O1.61 g, per 1000mL of seawater.
Further performing morphological and molecular biological identification on the strain.
Firstly, culturing the strain at 30 ℃ for 24 hours, and observing colony morphology; the specific method refers to the handbook of identifying common bacteria (Dongxiu bead, 2001) in combination with the measurement of physiological and biochemical indexes such as gram staining and carbon source utilization. As can be seen from FIG. 1, the strain MCW152 is gram-negative G-The cells are oval, the size of the cells is 1.5-1.6 mu m multiplied by 1-1.1 mu m, no polar hair is seen, and no spore is produced; culturing on solid DM plate for 24 hr, with colony size of about 1mm, round shape, smooth surface, convex middle, and creamy yellow color.
The DM flat plate is a denitrification performance determination culture medium and mainly comprises the following components: na (Na)2HPO4 7.9 g，KH2PO41.5g，MgSO4·7H20.1g of O, 2ml of trace element solution, KNO31.0g, 9.4g of sodium succinate and pH of 7.0-7.5, wherein the components of the trace element solution are the same as those of the trace element solution in the solid culture medium per 1000mL of seawater.
In addition, the strain obtained by the separation is positive to reduction reaction of oxidase and nitrate;
meanwhile, the inventor separates and obtains the genome DNA of the strain, performs PCR amplification by 16S rRNA gene, and sequences the obtained PCR amplification product, wherein the obtained sequence is shown in a sequence table; then, BLAST analysis is carried out by using an NCBI nucleic acid database according to the 16S rRNA gene sequence of the strain, the result shows that the similarity of the 16S rRNA gene sequence of the strain and Vibrio (Vibrio sp.) is up to 99%, the 16S rRNA partial gene sequence of 7 model strains of Vibrio sp. related to the strain is selected, a phylogenetic tree is constructed, the strain is shown to be positioned in the Vibrio sp. branch, and the strain is named as Vibrio (Vibrio sp.) strain MCW152 by the inventor in combination with gram staining and morphological observation.
Example 2
Optimum growth conditions for MCW152 strain:
the culture characteristics of the microbial strains comprise nutrient conditions for the growth of the strains, external factor conditions and the like, in order to improve the metabolic activity and denitrification capability of the functional strains, sufficient nutrition, proper temperature and good ventilation condition must be provided during culture, and the influence of the types and concentration of nutrients on the denitrification effect is also considered while the sufficient nutrition is provided for the functional strains, so that the additional carbon source, the culture conditions and the like are optimized so as to obtain better metabolic activity and denitrification effect.
1) Optimum carbon source:
respectively taking glucose, sodium acetate, sodium succinate, sodium oxalate and sodium citrate as carbon sources, keeping other components in the culture medium for determining the poor nutrient denitrification performance unchanged, inoculating and culturing bacterial liquid to a logarithmic phase according to 1 percent after sterilization, then culturing at 120rpm and 30 ℃, and respectively sampling for determining NO in the culture medium at 0h, 12h, 24h, 36 h and 48h3 --N、NO2 --N concentration and OD600And calculating the removal rate.
The nutrient-poor denitrification performance determination culture medium mainly comprises the following components: 0.05g of sodium nitrate, 0.05g of dipotassium hydrogen phosphate, 0.025g of calcium chloride and 0.025g of magnesium chloride. Carbon source: 0.25g of sodium acetate, 0.25g of sodium succinate, 0.3g of trisodium citrate, 0.18g of glucose and 0.41g of sodium oxalate, wherein each 1000mL of seawater is adopted.
As can be seen from FIGS. 2 and 3, the most suitable carbon source for strain MCW152 is sodium succinate, and when sodium succinate is used as the carbon source, strain MCW152 can react with NO3 -The removal rate of-N can reach 37.7% in 12h and 45.3% in 24 h.
2) Optimum temperature:
as can be seen from FIG. 4, glucose was used as a carbon source, and COD and NO were 200mg/L respectively3 -The culture medium for measuring the poor nutrition denitrification performance with the mass concentration of N of 10mg/L is inoculated with the MCW152 strain, the strain is respectively placed at 15 ℃, 25 ℃, 30 ℃, 35 ℃ and 40 ℃ and is continuously cultured for 24 hours under the condition of oscillation at 120rpm, and the NO in the culture solution is measured3 --N concentration.
The nutrient-poor denitrification performance determination culture medium mainly comprises the following components: 0.05g of sodium nitrate, 0.05g of dipotassium hydrogen phosphate, 0.025g of calcium chloride and 0.025g of magnesium chloride. Carbon source: 0.25g of sodium acetate, 0.25g of sodium succinate, 0.3g of trisodium citrate, 0.18g of glucose and 0.41g of sodium oxalate, wherein each 1000mL of seawater is adopted.
The results in FIG. 4 show that the MCW152 strain has a suitable growth and denitrification temperature range of 25-35 deg.C, and reaches the highest NO at 30 deg.C3 --N removal rate, NO at this time3 -The removal rate of-N is 73.4%, and in addition, it can be known from FIG. 4 that a critical temperature of 25 ℃ exists for the growth and denitrification of the MCW152 strain, and once the temperature is lower than the critical temperature, the growth and metabolic activity of the MCW152 strain is obviously reduced, so that the strain is thermophilic and very sensitive to low temperature, and 30 ℃ is recommended as the optimal growth temperature of the strain.
2) Optimum pH:
as can be seen from FIG. 5, glucose was used as a carbon source, and COD and NO were 200mg/L respectively3 --a poor nutrient denitrification performance measuring medium with an N concentration of 10mg/L, inoculating the MCW152 strain, continuously culturing for 12h under shaking table conditions of 30 ℃ and 120rpm with initial pH of 5.0, 6.0, 7.0, 8.0 and 9.0, and measuring NO in the culture solution3 --N concentration. The results in FIG. 5 show that the MCW152 strain grows cell pairs of NO3 -The metabolism removal of N and the cell growth thereof have higher activity in the pH range of 5-8, the optimal pH is 6, and NO is generated at the time3 -The removal rate of-N was 80.1%.
Example 3
Denitrification performance test of MCW152 strain:
setting the condition of the synthetic culture medium to the optimal condition, namely detecting the denitrification capability of the MCW152 strain by taking sodium succinate as a carbon source, controlling the temperature to be 30 ℃ and the pH to be 6;
specifically, the MCW152 strain was inoculated to NO3 -Shaking culture at 120rpm in synthetic medium with-N initial concentration of 150mg/L at 30 deg.C, and periodically sampling to determine NO3 --N and NO2 --concentration profile of N. As can be seen in conjunction with figure 6,the MCW152 strain can convert NO within 12h3 -The concentration of-N is reduced from above 150mg/L to below 0.05mg/L, and at the same time, NO is added2 -The concentration of-N is gradually increased and then gradually decreased to be below 0.05mg/L, which indicates that the MCW152 strain has high denitrification capability.
Example 4
Testing the denitrification performance of the MCW152 strain on the artificial simulated mariculture wastewater:
the mariculture wastewater has the following characteristics: (1) the discharge amount of the mariculture wastewater is large, but the types of pollutants are relatively few; (2) compared with industrial wastewater and domestic sewage, the concentration of pollutants in the mariculture wastewater is low, but the concentration of dissolved oxygen is high, and the mass concentration is generally more than 5.0 mg/L; the carbon-nitrogen ratio is low, generally 3-10, and far lower than the optimal carbon-nitrogen ratio of the microorganism.
The artificial simulated mariculture wastewater is preferably obtained by adding NaNO into seawater3、NaNO2、 KH2PO4One or more of the above, wherein the initial DO in the aquaculture wastewater is 4 mg/L-saturated, the pH is 6.5-8.5, the temperature is 20-30 ℃, and NaNO is preferably added into the seawater in the embodiment3The initial DO in the culture wastewater is 4mg/L, the pH is 7, and the temperature is 30 ℃;
the wastewater treatment device is preferably a sequencing batch wastewater treatment device, and the operating parameters of the sequencing batch wastewater treatment device are controlled as follows: the water inlet time is the addition of NaNO3The time for the wastewater to enter the sequencing batch wastewater treatment device is 1-5 min (preferably 3min in the embodiment), the standing is carried out for 6-10 h (preferably 7h in the embodiment) after the injection is finished, the time for the tail water to be treated to be discharged out of the device is 5-10 min (preferably 8min in the embodiment), the dissolved oxygen is less than 1.5mg/L, and the residence time of the wastewater in the device is controlled to be 6-12 h (preferably 8h in the embodiment).
In the embodiment, the effective capacity of the wastewater treatment container is preferably 2L, and 300-500 g (preferably 400g) of biodegradable polymers (BDPs) are added into the wastewater treatment container;
the adding amount of the MCW152 strain fermentation liquid in this embodiment is preferably 0.1 per mill of the volume of the wastewater, and the MCW152 strain fermentation liquid is added into the wastewater treatment container, the artificial simulated seawater culture wastewater is circulated and flowed in the wastewater treatment container by the circulating pump, fresh artificial simulated seawater culture wastewater is continuously added, the total nitrogen amount of the high-nitrogen low-carbon salt-containing wastewater is measured at the water inlet, and the total nitrogen amount of the treated high-nitrogen low-carbon salt-containing wastewater is measured at the water outlet.
After a period of time, a water quality comparison table of the water inlet and the water outlet shown in the table 1 is obtained;
TABLE 1 list of water quality at inlet and outlet (mg/L)
| Quality of water | TOC | TN | Ammonia nitrogen | Nitrate nitrogen | Nitrous nitrogen |
| Inflow water | 27.22 | 61.49 | 0.422 | 61.45 | 0.292 |
| Discharging water | 51.78 | 8.39 | 1.73 | 0.01 | 0.466 |
As can be seen from Table 1, TN at the water outlet of the system was less than 10mg/L, and the removal rate was 86.4%;
further, in order to verify that BDPs are utilized as a carbon source by microorganisms, biodegradable polymers (BDPs) were weighed before and after the above test, and it was found that the mass of the biodegradable polymers was reduced by about 8g in the test period; after the used biodegradable polymer particles are washed, dehydrated and dried, the particles and unused particles are sprayed with gold by a full-automatic ion sputtering instrument, and then the particles are placed under a scanning electron microscope for observation, the results are shown in figures 7 and 8, as can be seen from figure 7, the surfaces of unused BDPs carrier particles are smooth, as can be seen from figure 8, after a period of reaction, the surfaces of the BDPs carriers become uneven, and 1000 times of the surfaces of the BDPs carriers can be clearly seen to have many irregular protrusions and form many cracks under the scanning electron microscope, and the surfaces of the carriers before and after the reaction have great differences, so that the carriers are further verified to be utilized as carbon sources under the action of enzymes in microorganisms, namely, the biodegradable polymers (BDPs) can be used as solid growth promoters to provide nutrition for the system and supplement the carbon sources.
Example 5
Denitrification performance stability test of MCW152 strain immobilized on BDPs:
to better illustrate that the MCW152 strain is fixed on biodegradable polymers (BDPs) and has high and stable denitrification and purification capability, the sequencing batch wastewater treatment device in example 4 is left idle for about 3 weeks, the artificial simulated mariculture wastewater is reused to perform denitrification and purification tests and the total nitrogen content in the aquaculture wastewater is monitored, and as shown in fig. 9, even if the MCW152 strain fixed on the BDPs is left idle for a period of time, the MCW152 strain fixed on the BDPs still has high-efficiency denitrification capability, which indicates that the MCW152 strain fixed on the BDPs has good stability in the service cycle;
the MCW152 strain is adsorbed by active carbon to prepare a solid microbial inoculum, and is fed to fancy carps in a laboratory for 3 months, so that the growth condition of the fancy carps is good;
therefore, the MCW152 strain can be used for preparing a biological denitrification microbial inoculum and is used for solving the eutrophication problems such as overproof total nitrogen in a seawater aquaculture water body.
The above examples are merely preferred examples and are not intended to limit the embodiments of the present invention. In addition to the above embodiments, the present invention has other embodiments. All technical solutions formed by using equivalent substitutions or equivalent transformations fall within the protection scope of the claims of the present invention.
Sequence listing
<110> Wuhan Guanggu environmental protection science and technology, Inc. national Sedrin sea water desalination and comprehensive utilization institute
<120> vibrio strain for treating high-nitrogen low-carbon salt-containing wastewater and application thereof
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<160> 1
<170> SIPOSequenceListing 1.0
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<211> 1517
<212> DNA
<213> Vibrio sp.MCW152
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tcagatgtga aagcccgggg ctcaacctcg gaatagcatt tgaaactggc agactagagt 660
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accggtggcg aaggcggccc cctggacaga tactgacact cagatgcgaa agcgtgggga 780
gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgtctact tggaggttgt 840
ggccttgagc cgtggctttc ggagctaacg cgttaagtag accgcctggg gagtacggtc 900
gcaagattaa aactcaaatg aattgacggg ggcccgcaca agcggtggag catgtggttt 960
aattcgatgc aacgcgaaga accttaccta ctcttgacat ccagagaact ttccagagat 1020
ggattggtgc cttcgggaac tctgagacag gtgctgcatg gctgtcgtca gctcgtgttg 1080
tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta tccttgtttg ccagcgagta 1140
atgtcgggaa ctccagggag actgccggtg ataaaccgga ggaaggtggg gacgacgtca 1200
agtcatcatg gcccttacga gtagggctac acacgtgcta caatggcgca tacagagggc 1260
ggccaacttg cgaaagtgag cgaatcccaa aaagtgcgtc gtagtccgga ttggagtctg 1320
caactcgact ccatgaagtc ggaatcgcta gtaatcgtgg atcagaatgc cacggtgaat 1380
acgttcccgg gccttgtaca caccgcccgt cacaccatgg gagtgggctg caaaagaagt 1440
aggtagttta accttcgggg ggacgcttac cactttgtgg ttcatgactg gggtgaagtc 1500
gtaacaaggt agccgta 1517
Claims (4)
1. Vibrio (vibrio) for treating high-nitrogen low-carbon salt-containing wastewaterVibrio sp.) Strain MCW152 is characterized in that the wastewater is preserved in China center for type culture Collection with the preservation number of CCTCC NO. M2018030, and the carbon-nitrogen ratio of the high-nitrogen low-carbon salt-containing wastewater is less than or equal to 1.
2. Vibrio (Vibrio) of claim 1Vibrio spApplication of strain MCW152 in treatment of high-nitrogen low-carbon salt-containing wastewater, which is characterized in that the carbon-nitrogen ratio of the high-nitrogen low-carbon salt-containing wastewater is less than or equal to 1, and vibrio (Vibrio:)Vibrio spThe bacterial strain MCW152 is inoculated on a biodegradable polymer to supplement a carbon source and realize the treatment of the high-nitrogen low-carbon salt-containing wastewater, wherein the biodegradable polymer is a polyester molecule with the diameter of 3-5 mm.
3. Vibrio (Vibrio) of claim 1Vibrio spA method for treating high-nitrogen low-carbon salt-containing wastewater by using the strain MCW152 is characterized by comprising the following steps: it comprises the following steps: 1) putting the biodegradable polymer into a wastewater treatment container, standing for 3-7 days after the addition is finished, and then inoculating the vibrio (Vibrio)Vibrio sp.) Bacterial strain MCW152 bacterial liquid; the volume of the biodegradable polymer is 20-50% of the volume of the wastewater treatment container; the Vibrio bacteria (Vibrio spThe volume of bacterial liquid of the strain MCW152 is 0.1-1 per mill of the volume of the wastewater;
2) placing the wastewater treatment container in high-nitrogen low-carbon salt-containing wastewater which circularly flows; the carbon-nitrogen ratio of the high-nitrogen low-carbon salt-containing wastewater is less than or equal to 1;
3) respectively measuring the total nitrogen amount of the high-nitrogen low-carbon salt-containing wastewater at the water inlet and the total nitrogen amount of the high-nitrogen low-carbon salt-containing wastewater after being treated at the water outlet; nitrate nitrogen in effluent of the system is less than 0.05mg/L, and the removal rate is more than 99%; the total nitrogen is less than 10mg/L, and the removal rate is 86.4 percent.
4. A microbial agent for treating high-nitrogen low-carbon salt-containing wastewater, the active component of which is vibrio (vibrio) of claim 1Vibrio sp.) Strain MCW 152.
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