CN108330085A - A kind of fermentation process preparing probiotics suitable for the potassium decomposing bacterial strain of low temperature environment and based on the bacterial strain - Google Patents
A kind of fermentation process preparing probiotics suitable for the potassium decomposing bacterial strain of low temperature environment and based on the bacterial strain Download PDFInfo
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Abstract
The fermentation process of probiotics is prepared the invention discloses a kind of potassium decomposing bacterial strain suitable for low temperature environment and based on the bacterial strain, the bacterial strain is accredited as bacillus, bacterial strain number is BX, it is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC No.14769.The bacterial strain being capable of the effective insoluble inorganic mineral such as decomposing of potassium feldspar, apatite, increase the content of available nutrient in crop soil, promote crop growth, and the bacterium also has compared with strong solution potassium effect at low ambient temperatures, it can be used in High aititude and the soil improvement of north cold area, have good application value preparing biological organic fertilizer and soil conditioner etc..
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of suitable for the potassium decomposing bacterial strain of low temperature environment and based on this
Bacterial strain prepares the fermentation process of probiotics.
Background technology
Potassium is one of necessary three kinds of basic nutrition elements of crops, can promote crop growth, promotes the anti-of crop
Disease, resistance etc..Thus, potassium element is largely processed into potash fertilizer and is applied to agricultural.Soil is a natural potassium library, potassium content
It is very abundant, but 95% or more potassium is present in the form of slow state in the silicate minerals such as potassium feldspar or mica, cannot be made
The phenomenon that object is directly absorbed and utilized, and which results in soil not only rich potassium but also potassium deficiencys.
On the one hand, since China is convenient for the potassium resource utilized deficient, and distributed pole is uneven, to seriously restrict potash fertilizer
It produces and uses, there is 90% or more to rely on import in the potash fertilizer applied every year.It is shown according to soil survey information, there are about 70% in the whole nation
Arable land potassium deficiency, wherein 45% arable land severe potassium deficiency, and the ratio of China's Nitrogen, phosphorus, potassium is 1：0.4：0.16, it is remote small
In the 1 of developed country：0.5：0.4, these have become an important factor for limiting crop yield raising and quality improving.It is general at present
Not only of high cost all over the Chemical Potassium used, utilization rate is low and certain destruction is caused to ecological environment, and influences agricultural product
Quality.Therefore, development cost is relatively low, environmentally friendly Silicate Bacteria Fertilizer, to improving the micro- domain environment of soil and development
Green, the ecological agriculture have great theoretical and practical significance.
On the other hand, slightly solubility potassium aluminosilicate resource of the China based on potassium feldspar but very abundant, it is widely distributed, black
There are mineral reserve in the provinces such as Longjiang, Liaoning, Hebei, Henan, Jiangsu, Anhui, Hubei, Shaanxi, Sichuan, Yunnan, Guangdong, Guangxi, total to store up
Measure nearly 10,000,000,000 tons.Therefore, effective exploitation potassium feldspar resource, it will solve China's soluble potassium money therefrom to extract potassium manufacture potash fertilizer
The important channel of source present situation in short supply.Up to the present, researcher produces the utilization of potash fertilizer around insoluble potassic mineral resource
A variety of methods are had been presented for, sums up and is divided into high temperature method and wet method two major classes, these methods are because there are high energy consumption, dosages
Greatly, the shortcomings of production cost is high, and environmental pollution is serious fails to realize large-scale industrial production always.
Potassium is extracted with low energy consumption, cost is few, pollution-free, mild condition and method are simple from potassium feldspar using microbial method
The advantages such as single, are acknowledged as the extracting potassium from potash feldspar method of most future.Silicate bacteria is also known as B. mucilaginocus, and it is long can to decompose potassium
The aluminum silicates mineral such as stone, mica, therefrom release soluble potassium, are utilized for plant absorption.In addition its metabolite is such as
Hormonal substance, polysaccharide, organic acid etc. can inhibit growth of pathogenic bacteria, improve the micro- domain environment of soil, to be conducive to crops
Nutrient absorption, enhance crop disease-resistant ability, improve crop yield and improve crop quality.Therefore, screening potassium decomposing activity is higher
Silicate bacteria, study its potassium decomposing performance, certain theory and substance base can be provided for the preparation of Silicate Bacteria Fertilizer
Plinth.
Invention content
The present invention is directed to overcome the deficiencies of the prior art and provide a kind of potassium decomposing bacterial strain suitable for low temperature environment and based on should
Bacterial strain prepares the fermentation process of probiotics.
In order to achieve the above object, technical solution provided by the invention is as follows：
The efficient potassium decomposing bacterial strain that can adapt to low temperature environment is preserved in BeiJing, China in September in 2017 on the 28th
State's Microbiological Culture Collection administration committee common micro-organisms center preservation, preserving number are CGMCC No.14769, are named as
Bacillus BX bacterial strains, belong to bacillus.
Wherein, the potassium decomposing bacterial strain is suitble to growth temperature to be 18-37 DEG C, and it is 20-37 DEG C to be suitble to potassium decomposing temperature.
Preferably, the potassium decomposing bacterial strain optimum growth temperature is 35-37 DEG C, most suitable 28-30 DEG C of potassium decomposing temperature.
The bacterium colony rough surface of the potassium decomposing bacterial strain is opaque, the dirty white or yellowish of colony colour；Gram-positive bacteria；
Glucose, starch, sucrose, maltose, lactose, Glucomannan, galactolipin can be utilized；Energy decomposition glucose generates pyruvic acid,
It decomposes tryptophan and forms indoles, starch can be hydrolyzed, there is lecithinase hydrolysis ability.
The potassium decomposing bacterial strain and crop are environmentally friendly to crop and crop without symbiosis feature.By degrading, soil is insoluble
Aluminosilicate inorganic minerals to improve the content of the available potassium of plant in soil.
The solid fermentation method for preparing microecological microbial agent based on above-mentioned potassium decomposing bacterial strain includes the following steps：
(1) picking potassium solubilizing bacteria BX is inoculated into LB liquid medium, the shake culture at 37 DEG C, culture to bacterium solution OD600=
0.5, obtain potassium decomposing bacteria culture fluid；
(2) potassium decomposing bacteria culture fluid is transferred in fermentation tank, 20-28h of aerlbic culture is preferably for 24 hours obtained at 37 DEG C
Strain liquid seed；
(3) bacterial strain seed liquor is inoculated into the solid wheat bran of high-temperature sterilization, the matter of bacterial strain seed liquor and solid wheat bran
Amount cultivates 24-48h, until viable count reaches 4.0 × 10 than being 8-12%, preferably 10% at 25-37 DEG C10Cfu/g,
The microbial inoculum of viable count requirement must be reached；
(4) microbial inoculum is dried to water content≤5% to get potassium solubilizing bacteria microecological microbial agent；It is preferred that dry under the conditions of 50 DEG C.
It is that potassium decomposing bacterial strain is seeded to liquid training based on the liquid fermentation method that above-mentioned potassium decomposing bacterial strain prepares probiotics
Base is supported, 20-28h of fermented and cultured under the conditions of 37 DEG C, rotating speed 180rpm preferably for 24 hours obtains the zymotic fluid for soil potassium decomposing,
As potassium solubilizing bacteria microecological microbial agent；Alternatively, the dried object of soil potassium decomposing, as potassium solubilizing bacteria must be used for after zymotic fluid is dried
Microecological microbial agent；The fluid nutrient medium is grouped as by the group of following parts by weight：Glucose 8-12, MgSO4·7H2O 0.3—
0.7, Na2HPO41.5-2.5, CaCO30.3-0.7, FeCl20.008-0.012, (potassium is long for feldspar in powder 0.8-1.2
Mountain flour crosses 40 mesh sieve, with deionized water soaked overnight, is then rinsed repeatedly with deionized water again, removes solubility therein
Potassium, 50 DEG C of dryings), deionized water 950-1000, pH 7.0-7.5.
Preferably, the fluid nutrient medium is grouped as by the group of following parts by weight：Glucose 10, MgSO4·7H2O 0.5,
Na2HPO42, CaCO30.5, FeCl20.01, feldspar in powder 1, deionized water 1000, pH 7.2.
Above-mentioned potassium decomposing bacterial strain can be used for preparing Biologically-fermentorganic organic fertilizer, can be also used for preparing soil conditioner, modifying agent
Or agent of loosening the soil.In use, can granule, pulvis, solution, suspension or stylus be prepared into efficient potassium solubilizing bacteria, can with it is organic
Fertilizer is used in mixed way, and can also be used alone, and rhizosphere microorganism environment is improved.
In soil of the potassium solubilizing bacteria BX of the present invention screened from Tibet river valley highland barley plantation area, after soil sample is suitably diluted
It is coated in screening and culturing medium, isolates the bacterium colony with high vigor ability of dissolving potassium, access test tube slant, obtain several potassium decomposings
Bacterium；Shaking flask liquid fermentation carries out secondary screening, measures potassium decomposing vigor, obtains the highest bacterial strain BX of potassium decomposing vigor；The measurement of potassium decomposing vigor
Mainly by measuring K in the front and back culture solution of fermentation+Concentration difference is evaluated, and potassium bacterium BX is inoculated in the training of 100mL liquid
It supports in base, 37 DEG C, shaken cultivation 2 days under the conditions of 7.4,160 rpm of pH takes culture 10ml, 2ml 6%H are added2O2, boiling water
1h, the K of atomic absorption spectroscopy determination digestive juice are digested in bath+Concentration simultaneously calculates bacterial strain and divides potassium feldspar miberal powder
Solution rate.
Compared with prior art, beneficial effects of the present invention are：
1. bacterial strain Bacillus BX provided by the invention and its fermented and cultured are prepared potassium decomposing microbial inoculum and can effectively decompose
The insoluble aluminosilicate inorganic minerals such as potassium feldspar, apatite promote the nutrient elements such as potassium, phosphorus, silicon, the magnesium of slightly solubility to turn
It is melted into soluble nutrient, increases the content of available nutrient in soil, promotes crop growth, improves yield.To giving full play to
Soil ecology fertility keeps the balance etc. of agroecological environment to all have meaning and application value of crucial importance.
It is a kind of environment-friendly type fertilizer 2. potassium decomposing microbial inoculum provided by the invention is not present in production process to environmental emission pollutant
Material；And the preparation method of potassium decomposing microbial inoculum provided by the invention, simple production process, production efficiency is high, can realize that industrialization is big raw
Production has good economic benefits and social effect.
Specific implementation mode
The screening of 1 potassium solubilizing bacteria of embodiment with detach
Pedotheque number part is acquired from Tibet Autonomous Region's potassium feldspar mining area, is stored in aseptic plastic bag, air transport is stood after returning
Detach.It under sterile working, weighs 1.0g soil samples and is dissolved in 9ml sterile salines, in addition bead, 28 DEG C of shaking tables
After 180rpm fully vibrates 30min, gradient dilution is coated on produced isolation medium tablet.It puts upside down in 28-30 DEG C
It is cultivated 2-3 days in insulating box, whole of edge, moistening, sticky, water white transparency and flexible bacterium colony is provoked with transfer needle, into
The separation of one step, purifying.Bacterial strain after purification is transferred to the screening and culturing medium tablet that potassium feldspar is unique potassium resource, 28 DEG C of cultures
4d, picking is well-grown, large size is smooth, round, transparent, surface wettability is sticky, flexible bacterium colony carries out inclined-plane preservation.
Primary dcreening operation bacterial strain is connected to potassium decomposing and measures in culture medium, is covered with tetraphenylboron sodium (+2% potassium hydroxide of 1% tetraphenylboron sodium)
Tablet, and 0.04% bromophenol blue colour developing is added, periphery of bacterial colonies becomes the bacterial strain bacterial strain as a purpose of sapphirine.
Above-mentioned culture medium prescription is as follows, isolation medium：Glucose 10.0g, KH2PO40.2g, MgSO4·7H2O
0.2g, NaCl 0.2g, CaCO35.0g, deionized water 1000mL, pH=7.2；Screening and culturing medium：Glucose 10.0g,
MgSO4·7H2O 0.5g, Na2HPO42g, CaCO30.5g, FeCl2(feldspar in powder crosses 40 mesh by 0.01g, feldspar in powder 1.0g
Sieve, with deionized water soaked overnight, is then rinsed with deionized water repeatedly again, removes the potassium of solubility therein, and 50 DEG C dry
It is dry), deionized water 1000 mL, pH are adjusted to 7.2.
Growth medium：Glucose 10.0g, MgSO4·7H2O 0.5g, CaCO31.0g, (NH4)2SO41.0g, NaCl
0.1g, Na2HPO42.0g, feldspar in powder 2.5g (method is same as above), pH 7.2, deionized water 1000mL.
The measurement of 2 bacterial strain ability of dissolving potassium of embodiment
The inoculation that screening is obtained is inoculated in potassium decomposing in 100mL growth mediums, with 2% inoculum concentration and measures culture
Base, control group access equivalent inactivated bacterial liquid, in 28 DEG C, 160rpm shaken cultivations 5d.Strain cultured solution 10ml is taken, 6%H is added2O2
2ml digests 1h in boiling water bath, and above-mentioned digestive juice is centrifuged 20min in 4000rpm, supernatant is taken, using Atomic absorption point
Light photometry measures K+Concentration calculates resolution ratio of the bacterial strain to potassium feldspar miberal powder.
Potassium decomposing rate calculation formula is as follows：
In the bacterial strain that screening obtains, the ability of dissolving potassium of BX bacterial strains is highest, K in zymotic fluid+Concentration is control group
3.16 again.
The identification of 3 potassium solubilizing bacteria BX of embodiment
Bacterial strain BX is measured in sole carbon source or the growing state of only nitrogen source culture medium, colonial morphology is observed, obtains potassium decomposing
The morphological feature of bacterium BX.Bacterium colonies of the bacterial strain BX on solid medium is faint yellow, round, and rough surface is opaque, and diameter is about
5mm, central protrusion.Microscopically observation, the bacterium are rod-short, Gram-negative.Glucose, starch, sugarcane can be utilized
Sugar, maltose, lactose, Glucomannan, galactolipin；Energy decomposition glucose generates pyruvic acid, decomposes tryptophan and forms indoles, can water
Starch is solved, there is lecithinase hydrolysis ability.Cracking process extracts the total DNA of bacterial strain BX, amplifies 16s rDNA and carries out bacterium point
Son identification, amplified production through the Shanghai bio tech ltd Mai Pu be sequenced, and with GenBank number sequence analysis, be determined as
Bacillus sp.。
4 potassium solubilizing bacteria Bacillus BX solid fermentations of embodiment produce probiotics
Picking potassium solubilizing bacteria BX is inoculated into 100mL LB liquid mediums, the shake culture at 37 DEG C, culture to bacterium solution
OD600=0.5.Then above-mentioned potassium decomposing bacteria culture fluid is transferred to 10mL fermentation tanks, aerlbic culture 24 hours, obtain bacterium at 37 DEG C
Strain liquid seeds.Then liquid seeds are inoculated into the 100kg solid wheat bran of high-temperature sterilization, inoculum concentration mass ratio is
10%.24-48h are cultivated at 25-37 DEG C, until the viable count in solid complex carrier reaches 4.0 × 1010cfu/g.It is up to work
The microbial inoculum that bacterium number requires is dried at 50 DEG C to water content≤5%, you can obtains potassium decomposing microbial inoculum.
5 potassium solubilizing bacteria Bacillus BX liquid fermentations of embodiment produce probiotics
Liquid Culture based formulas is glucose 10.0g, MgSO4·7H2O 0.5g, Na2HPO42g, CaCO30.5g,
FeCl2(feldspar in powder crosses 40 mesh sieve, with deionized water soaked overnight, then uses deionized water again by 0.01g, feldspar in powder 1.0g
Rinse repeatedly, remove the potassium of solubility therein, 50 DEG C of dryings), deionized water 1000mL, pH 7.2.Cultivation temperature is 37
DEG C, rotating speed 180rpm, incubation time for 24 hours, obtains potassium decomposing of the zymotic fluid for soil, or processing is dried in zymotic fluid and is obtained
Dried object is used for the potassium decomposing of soil.
Claims (10)
1. a kind of efficient potassium decomposing bacterial strain can adapt to low temperature environment, which is characterized in that the potassium decomposing bacterial strain is in the micro- life of China
The common micro-organisms center preservation of object culture presevation administration committee, preserving number are CGMCC No.14769, are named as Bacillus
BX bacterial strains.
2. potassium decomposing bacterial strain as described in claim 1, which is characterized in that the potassium decomposing bacterial strain is suitble to growth temperature to be 18-37
DEG C, it is 20-37 DEG C to be suitble to potassium decomposing temperature.
3. potassium decomposing bacterial strain as claimed in claim 2, which is characterized in that the potassium decomposing bacterial strain optimum growth temperature is 35-37
DEG C, most suitable 28-30 DEG C of potassium decomposing temperature.
4. potassium decomposing bacterial strain as described in claim 1, which is characterized in that the bacterium colony rough surface of the potassium decomposing bacterial strain is opaque,
The dirty white or yellowish of colony colour；Gram-positive bacteria；Glucose, starch, sucrose, maltose, lactose, Portugal can be utilized sweet
Glycan, galactolipin；Energy decomposition glucose generates pyruvic acid, decomposes tryptophan and forms indoles, can hydrolyze starch, has lecithinase
Hydrolysis ability.
5. potassium decomposing bacterial strain as described in claim 1, which is characterized in that the potassium decomposing bacterial strain and crop are without symbiosis feature, to making
Object and crop are environmentally friendly.
6. preparing the solid fermentation method of microecological microbial agent based on any one of claim 1 to the 5 potassium decomposing bacterial strain, feature exists
In described method includes following steps：
(1) picking potassium solubilizing bacteria BX is inoculated into LB liquid medium, the shake culture at 37 DEG C, culture to bacterium solution OD600=0.5,
Obtain potassium decomposing bacteria culture fluid；
(2) potassium decomposing bacteria culture fluid is transferred in fermentation tank, 20-28h of aerlbic culture at 37 DEG C obtains strain liquid seed；
(3) bacterial strain seed liquor is inoculated into the solid wheat bran of high-temperature sterilization, the mass ratio of bacterial strain seed liquor and solid wheat bran
It is 8-12%, 24-48h is cultivated at 25-37 DEG C, until viable count reaches 4.0 × 1010Cfu/g must reach viable count requirement
Microbial inoculum；
(4) microbial inoculum is dried to water content≤5% to get potassium solubilizing bacteria microecological microbial agent.
7. preparing the liquid fermentation method of probiotics based on any one of claim 1 to the 5 potassium decomposing bacterial strain, feature exists
In the method is that potassium decomposing bacterial strain is seeded to fluid nutrient medium, the fermented and cultured 20-under the conditions of 37 DEG C, rotating speed 180rpm
28h obtains the zymotic fluid for soil potassium decomposing, as potassium solubilizing bacteria microecological microbial agent；Alternatively, must be used for after zymotic fluid is dried
The dried object of soil potassium decomposing, as potassium solubilizing bacteria microecological microbial agent；The fluid nutrient medium is grouped as by the group of following parts by weight：Portugal
Grape sugar 8-12, MgSO4·7H2O 0.3-0.7, Na2HPO41.5-2.5, CaCO30.3-0.7, FeCl2 0.008—
0.012, feldspar in powder 0.8-1.2, deionized water 950-1000, pH 7.0-7.5.
8. the method for claim 7, which is characterized in that the fluid nutrient medium is grouped as by the group of following parts by weight：
Glucose 10, MgSO4·7H2O 0.5, Na2HPO42, CaCO30.5, FeCl20.01, deionized water 1000, pH 7.2.
9. application of the potassium decomposing bacterial strain in preparing Biologically-fermentorganic organic fertilizer as described in any one of claim 1 to 5.
10. potassium decomposing bacterial strain the answering in preparing soil conditioner, modifying agent or agent of loosening the soil as described in any one of claim 1 to 5
With.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111748485A (en) * | 2020-04-08 | 2020-10-09 | 惠州学院 | Screening method of silicate bacteria with high potassium-decomposing capacity suitable for low-temperature slightly-acidic environment and potassium-decomposing bacterial fertilizer |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061274A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus mucilaginosus with potassium-decomposing function and application thereof |
| CN104232538A (en) * | 2014-09-03 | 2014-12-24 | 南京聚肽高科农业有限公司 | Efficient potassium bacterium and application thereof |
| CN107227265A (en) * | 2016-10-28 | 2017-10-03 | 武汉合缘绿色生物股份有限公司 | A kind of colloid bacillus cereus bacterial strain and microbial bacterial agent |
-
2018
- 2018-01-30 CN CN201810086647.9A patent/CN108330085B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061274A (en) * | 2010-11-05 | 2011-05-18 | 南开大学 | Bacillus mucilaginosus with potassium-decomposing function and application thereof |
| CN104232538A (en) * | 2014-09-03 | 2014-12-24 | 南京聚肽高科农业有限公司 | Efficient potassium bacterium and application thereof |
| CN107227265A (en) * | 2016-10-28 | 2017-10-03 | 武汉合缘绿色生物股份有限公司 | A kind of colloid bacillus cereus bacterial strain and microbial bacterial agent |
Non-Patent Citations (1)
| Title |
|---|
| 谢庆东，等: "一株高效溶解钾长石盐杆菌的分离鉴定与生物学特性研究", 《土壤》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111748485A (en) * | 2020-04-08 | 2020-10-09 | 惠州学院 | Screening method of silicate bacteria with high potassium-decomposing capacity suitable for low-temperature slightly-acidic environment and potassium-decomposing bacterial fertilizer |
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