CN108329372B - N 6 Application of- (2-hydroxyethyl) adenosine and derivatives thereof in preparation of medicines for treating gout - Google Patents

Abstract

The present invention provides N ⁶ Use of (2-hydroxyethyl) adenosine for the treatment of gout conditions. Cordyceps extract N ⁶ - (2-hydroxyethyl) adenosine has a good analgesic effect by activating adenosine a1 receptor. By administering gout rats N ⁶ - (2-hydroxyethyl) with 932 genes differentially expressed in the synovial membrane of the joints, 57 being pain-related genes and 12 being pain-relief-related genes. N is a radical of hydrogen ⁶ - (2-hydroxyethyl) adenosine obviously reduces the swelling degree of joints, the levels of interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) in serum and joint cleaning solution, reduces the levels of NLRP3 inflammatory corpuscle and caspase 1(caspase-1), promotes autophagy and p62 degradation, and plays a role in resisting inflammation and easing pain. Can be used for relieving pain and inflammation caused by gout.

Description

N 6 Application of- (2-hydroxyethyl) adenosine and derivatives thereof in preparation of medicines for treating gout

Technical Field

The invention relates to the technical field of medicines, and belongs to application of an active substance extracted from cordyceps fungi (or artificially synthesized) to anti-inflammation and analgesia in gout diseases.

Background

Gout is a metabolic disease caused by the fact that the blood uric acid concentration is continuously increased to cause urate crystals to deposit on soft tissues. Gout is developed in an acute stage with red swelling and hot pain, which is more than that developed in the middle of the night, the pain can cause a person to wake up from sleep, the affected joints are more like metatarsophalangeal joints or thumbs, single arthritis usually occurs in the early stage of the development, local hyperalgesia and limited activity occur after hours, and disability can be caused in the later stage. The incidence rate of the traditional Chinese medicine is increased by 1-2% year by year and is low in age (So 2008), and huge burden is caused to the life of people. Colchicine and nonsteroidal anti-inflammatory drugs are commonly used for treating acute gout attack in clinic, but the use is limited due to side effects such as renal insufficiency, gastrointestinal bleeding and heart failure (Neogi 2011).

The concentration of natriuretic sodium continues to be higher than its solubility in blood to precipitate and deposit on the distal extremity such as the metatarsophalangeal, finger, etc., where blood flow is low or local temperature is low, stimulating local synovial cells, macrophages and neutrophils to produce a series of inflammatory factors such as tumor necrosis factor alpha (TNF- α), interleukin 1 β (IL-1 β), interleukin 6(IL-6) and interleukin 8(IL-8), thereby augmenting the inflammatory response and disrupting local cellular homeostasis.

Cellular homeostasis is regulated by autophagy, an evolutionarily highly conserved process. Autophagy can be induced by a variety of conditions, such as stress on organelles or antigen infection, among others. Autophagy can find and clear the body's own misfolded proteins and damaged or dysfunctional organelles to control the cell in a relatively stable state. There is evidence that autophagy is associated with a variety of human diseases, such as tumors, diabetes, neurodegenerative diseases, and osteoarthritis. In addition, autophagy has been reported to be associated with gout, and after treatment of neutrophils with synovial fluid or sodium urate crystals from gout patients in acute episodes, the cells produce neutrophil extracellular traps in an autophagy-dependent manner, which are associated with inhibition of inflammation.

p62, a multifunctional ubiquitin-binding protein, is involved in the degradation of two proteins, the ubiquitin-proteasome system and the autophagy-lysosome system. Mediates a variety of cellular functions, and in particular plays an important role in selective autophagy and cellular antioxidant responses of cells, and thus p62 is intimately involved in the pathogenesis of many diseases, including metabolic syndrome, neurodegenerative diseases, muscle diseases, and gout.

Cordyceps cicadae Ophiocerceps sobolifera G.H.Sung, which has been used as a traditional Chinese medicine in China for 1600 years. The pharmacological activity of the compound for treating chronic blepharitis and acute conjunctivitis, which is recorded in Taiping Huimin and He Ji Ju Fang, of sedation and analgesia is also reported. N is a radical of hydrogen ⁶ - (2-hydroxyethyl) adenosine (HEA) is an active compound separated from cordyceps cicadae, and although the anti-inflammatory and analgesic effects are reported, the anti-inflammatory and analgesic effects are not reported in the field of gout treatment.

Disclosure of Invention

Adenosine receptors are a class of G protein-coupled receptors, and are divided into four subtypes, a1, A2A, A2B, and A3. The present group found that the adenosine A1 receptor (A1R) and adenosine A2A receptor (A2AR) are closely related to the transmission and modulation of pain information, encoded by Adora1 and Adora2a genes, respectively. A large number of experiments prove that the activated adenosine A1 receptor plays an analgesic role in postoperative pain, inflammatory pain, arthralgia and neuropathic pain.

The transcriptome sequencing adopted by the invention is a high-throughput gene expression analysis method, and can comprehensively understand the gene expression change in the disease occurrence process and the treatment process by analyzing the difference among the gene expressions of a normal sample, a modeling sample (disease state) and a drug administration sample (treatment state), screen potential pathogenic genes or drug treatment targets, and provide a direction for new drug research and development and disease monitoring.

In one aspect, the invention provides the use of N6- (2-hydroxyethyl) adenosine as an anti-inflammatory analgesic in the treatment of gout.

In another aspect, N6- (2-hydroxyethyl) adenosine is used for anti-inflammatory and analgesic use in the treatment of acute gout.

In some embodiments, the anti-inflammatory use comprises use to reduce tumor necrosis factor alpha and interleukin beta.

In some embodiments, wherein the anti-inflammatory use comprises use in a formulation that reduces the level of swelling in joints.

In some embodiments, wherein said anti-inflammatory use comprises use in a formulation for reducing the level of expression of inflammasome.

In some embodiments, wherein the analgesic use comprises use of up-regulating adenosine a1 receptor, adenosine A2A receptor mRNA expression, up-regulating adenosine a1 receptor protein expression, or reducing adenosine A2A receptor protein expression.

In another aspect, the invention provides the use of N6- (2-hydroxyethyl) adenosine in the preparation of a formulation for promoting autophagy levels in the treatment of gout.

Use of N6- (2-hydroxyethyl) adenosine in the manufacture of a formulation for promoting degradation of p62 in the treatment of gout.

In some embodiments, the use to promote levels of autophagy comprises use to upregulate the expression of adenosine a1 receptor.

In some modes, cordyceps mycelia, sporophores and spores containing N6- (2-hydroxyethyl) adenosine are directly or indirectly separated from cordyceps mycelia, sporophores and spores containing N6- (2-hydroxyethyl) adenosine or artificially synthesized, wherein the cordyceps mycelia, sporophores and spores containing N6- (2-hydroxyethyl) adenosine are directly used.

In another aspect, the invention relates to a formulation for reducing the levels of tumor necrosis factor alpha and interleukin beta for use in the treatment of gout or acute gout, wherein the formulation comprises N6- (2-hydroxyethyl) adenosine.

In some embodiments, a formulation for reducing the level of swelling in joints in the treatment of gout or acute gout, wherein the formulation comprises N6- (2-hydroxyethyl) adenosine.

In some embodiments, a formulation for reducing inflammatory body expression levels for use in treating gout or acute gout, wherein the formulation comprises N6- (2-hydroxyethyl) adenosine.

In some forms, a formulation for up-regulating adenosine a1 receptor expression levels in the treatment of gout or acute gout, wherein the formulation comprises N6- (2-hydroxyethyl) adenosine.

In some embodiments, the formulation may or may not include other active ingredients.

In some embodiments, the composition further comprises a pharmaceutically acceptable excipient.

In a further aspect, the invention provides the use of N6- (2-hydroxyethyl) adenosine in the preparation of a gout drug.

In all of the foregoing embodiments, N6- (2-hydroxyethyl) adenosine has the structural formula shown in formula I:

in another aspect, the invention provides a therapeutic or analgesic agent, wherein the agent comprises N6- (2-hydroxyethyl) adenosine.

In any of the foregoing modes, wherein the N6- (2-hydroxyethyl) adenosine is operated by the regulatory gene sequences SEQ ID No.1 to SEQ ID No. 59. The effect is analgesia.

In some embodiments, wherein the N6- (2-hydroxyethyl) adenosine is acted upon by the regulatory gene sequences SEQ ID No.48 to SEQ ID No. 59. The effect is analgesic.

In other aspects, the invention provides a gene sequence involved in HEA regulation, the gene sequence comprising a sequence according to any one of SEQ ID No.1 to SEQ ID No.59, the expression of which is associated with pain.

In other aspects, the invention provides a gene sequence involved in HEA regulation, the gene sequence comprising a sequence selected from one of SEQ ID No.48 to SEQ ID No.59, the expression of which is associated with pain.

In other embodiments, the genes are one or more sequences comprising c1qb (SEQ ID No. 48); c1qc (SEQ ID No. 49); timp1(SEQ ID No. 50); lum (SEQ ID No. 51); csrp3(SEQ ID No. 52); stmn4(SEQ ID No. 53); uchl1(SEQ ID No. 54); ctsk (SEQ ID No. 55); lxn (SEQ ID No. 56; Nsf (SEQ ID No. 57); Adora1(SEQ ID No. 58); Adora2a (SEQ ID No. 59).

In some embodiments, the N6- (2-hydroxyethyl) adenosine of the present invention can be isolated from Cordyceps fungus producing N6- (2-hydroxyethyl) adenosine, such as Cordyceps sobolifera (o. sobolifera), Cordyceps militaris (cordyces militaris), Cordyceps sinensis (cordyces pruinosa), Cordyceps sinensis (ophiocordyces sinensis), and the like. N6- (2-hydroxyethyl) adenosine can be obtained by culturing the above Cordyceps fungi such as Paecilomyces cicadae, and can be isolated from fruiting body, mycelium, spore, and mycelium culture medium. N6- (2-hydroxyethyl) adenosine can also be obtained from artificial chemical synthesis, and can also be directly purchased through commercial products.

This function could be demonstrated in rats and this novel use of N6- (2-hydroxyethyl) adenosine could also be used in humans or mammals for the treatment of gout. Therefore, the invention provides a preparation for treating gout, wherein the preparation comprises N6- (2-hydroxyethyl) adenosine.

The invention discloses the anti-inflammatory analgesic action and mechanism of cordyceps sobolifera active ingredient N6- (2-hydroxyethyl) adenosine (HEA) by adopting a sodium urate (MSU) crystal ankle joint injection rat gout model, and provides a theoretical basis for the application and development of the anti-inflammatory analgesic action of cordyceps sobolifera and other cordyceps containing the cordyceps sobolifera.

HEA is reported to have anti-inflammatory effects, but its anti-inflammatory effects have not been reported on gout models. The invention adopts a rat gout model induced by sodium urate to explain the anti-inflammatory mechanism of HEA.

The abdominal injection of HEA (5,7.5,10mg/Kg) can obviously relieve gout ankle swelling induced by the MSU of rats. The inflammatory swelling method is the most common acute non-specific inflammation model in anti-inflammatory drug experiments, and is an intuitive, simple and easy-to-operate inflammatory indicator.

Enzyme-linked immunosorbent assay is used for finding that the intraperitoneal injection of HEA can reduce the levels of TNF-alpha and IL-1 beta in rat blood and joint cleaning solution, wherein the HEA (7.5mg/Kg) has the most obvious effect and the best significance, so that the concentration is used for the next experiment. IL-1 beta is a key inflammatory factor for gout attack, and IL-1 beta knockout mice can completely resist MSU-induced gout attacks. TNF- α is a pro-inflammatory cytokine produced primarily by macrophages and monocytes and is involved in normal inflammatory and immune responses. TNF-alpha and IL-1 beta levels are important basal indicators indicating the degree of inflammation.

Protein expression levels of cysteine aspartate specific protein binding enzyme 1(Caspase-1), NLRP3 inflammasome, p62 and LC3II in synovial membranes of rat joints are detected by a Western Blot method. Caspase-1 is activated by binding to the inflammasome, processing the inflammatory factors such as IL-1 β precursor to mature and release, playing a central role in the regulation of inflammatory responses. The inflammasome is capable of regulating the activation of caspase-1 (caspase-1) and thus promoting the cleavage maturation of the cytokine precursors pro-IL-1 β and pro-IL-18 during the course of the innate immune defense. It also regulates caspase-1 dependent form apoptosis, inducing cell death under inflammatory and stress pathological conditions. LC3II is an indicator protein for the number of autophagosomes. Intraperitoneal injection of HEA (7.5mg/Kg) reduced the level of NLRP3, Caspase-1, LC3II, and p62 in the synovium. A decrease in LC3II levels means a decrease in the number of autophagosomes, and a decrease in the number of autophagosomes means a decrease in the production of autophagosomes due to a decrease in autophagy levels or an increase in degradation of autophagosomes due to an increase in autophagy levels. The p62 protein level decreased, while the transcript level was not different, indicating that HEA promoted degradation of p 62.

To explore the specific effect of HEA (7.5mg/Kg) on autophagy, chloroquine, which inhibits degradation of autophagosomes, was introduced in this study. The results indicate that HEA (7.5mg/Kg) can restore the MSU-induced suppressed status of autophagosomal degradation, thereby promoting autophagy flow. In conclusion, HEA has anti-inflammatory effect on gout model, and the action mechanism of HEA is related to the inhibition of TNF-alpha and IL-1 beta inflammatory factor level, NLRP3 inflammasome, promotion of autophagy flow and p62 degradation.

The cordyceps cicadae miq has a strong analgesic effect, and the invention adopts an activity tracking method to distribute and extract a substance from the cordyceps cicadae miq step by step and determine the pain inhibition rate of the substance on an acetic acid induced pain mouse model, and finally determines that the active substance of the cordyceps cicadae miq is HEA, so that the cordyceps cicadae miq has a strong analgesic effect, and the pain inhibition rate reaches 99%.

In order to fully reveal the analgesic mechanism of HEA, the present study observed the transcriptome effect of HEA on the synovial membrane of ankle joint of gout model rat, and the results showed that, after applying HEA (7.5mg/Kg) by intraperitoneal injection, 932 genes were differentially expressed compared to the model group, and combined with the online pain gene database, 57 differential genes involved in pain regulation were obtained, and then combined with literature reports, 12 analgesic genes were finally obtained, which were related to the effect of HEA, including down-regulated complement component 1q subcomponent b (c1qb), complement component 1q subcomponent c (c1qc), metalloproteinase tissue inhibitor 1(Timp1), luminal protein (Lum), cysteine and glycine-rich protein 3(Csrp3), Stahmin-like protein 4(Stmn4), ubiquitin carboxy-terminal hydrolase L1(Uchl1), cathepsin K (CtsK), latex (Lxn), and up-regulated Nsff molecule (N-protein), nsf), adenosine A1 receptor (A1R, Adora1), and adenosine A2A receptor (A2AR, Adora2 a).

The adenosine A1 receptor (A1R) and adenosine A2A receptor (A2AR) are intimately involved in the information transfer and regulation of pain. The protein expression of A1R and A2AR was detected by Western Blot, and the results showed that A1R and A2AR in the model group were higher than those in the blank control group. Compared with the blank group, the HEA (7.5mg/Kg) administration group has obviously increased A1R (P <0.01) and obviously decreased A2AR (P < 0.01); compared with the model group, A1R is also increased (P <0.05), and A2AR is obviously decreased (P < 0.01). After DPCPX (2mg/Kg) and HEA (7.5mg/Kg) are applied simultaneously, the A1R protein expression is obviously reduced, and no statistical difference is generated compared with blank and model groups. In conclusion, the analgesic mechanism of HEA is related to agonizing A1R, down-regulating A2AR and regulating a series of pain genes.

Drawings

FIG. 1 shows the joint swelling rates of the model group, different concentrations of HEA (5,7.5,10mg/Kg), colchicine (Col) group, and normal group at each time point after the model creation of rats.

FIG. 2 shows the TNF-. alpha.and IL-1. beta.levels in blood of the model group, the different concentrations of HEA group (5,7.5,10mg/Kg), the colchicine group (Col), and the normal group at each time point after rat molding.

FIG. 3 shows TNF-. alpha.and IL-1. beta. levels in joint wash of model group, different concentrations of HEA group (5,7.5,10mg/Kg), colchicine group (Col), and normal group at various time points after rat molding.

FIG. 4 shows the protein expression levels of Caspase-1, NLRP3 inflammasome, p62, LC3II in synovial membrane of rat joints (3-MA is autophagy blocker).

FIG. 5 shows the expression of LC3II protein in the normal group, model group, HEA administration group, and three groups of chloroquine group.

FIG. 6 shows the pain suppression rates of the stepwise extracted substances CYD, CYDd-2A (HEA).

FIG. 7 shows up-regulated gene portions of 57 genes involved in pain regulation and expression profiles thereof (the larger the value, the more the gene expression).

FIG. 8 is a down-regulation portion of a graph of 57 genes involved in pain regulation and their expression levels (the larger the number, the more gene expression).

FIG. 9 is a diagram of the expression of A1R and A2AR receptors in synovial membrane of rat ankle joint. Comparison with model group, # comparison with blank group (DPCPX is a selective antagonist of A1R).

FIG. 10 shows the pain inhibition rate of HEA and its derivatives B-H. A is HEA, B-H is HEA derivative, and the specific structure formula is shown in example 3

FIG. 11: structure of different derivatives of HEA.

Detailed Description

The embodiments of the present invention are only direct indications of the novel functions of the compounds mentioned in the present invention as one embodiment of the present invention, but are not intended to limit the present invention.

Materials used in the practice of the invention

Experimental animals Male SD rats, 180-220g, purchased from animal Experimental center of Wenzhou medical school. The quality certification serial number of the experimental animal is as follows: SCXK (Zhe) 2010-0150. The Kunming white mouse, male and female account for 50% respectively, has a weight of 18-22g, and is provided by Shanghai pharmaceutical research institute of Chinese academy of sciences. Rats and mice were acclimatized for at least 7 days in a standard breeding environment (12h light dark cycle; temperature 22 + -2 deg.C; relative humidity, 50% + -5%) and were given free access to standard feed and drinking water, with only one experiment per mouse.

Drugs and Agents Cordyceps cicadae was supplied by the institute for subtropical crops in Zhejiang province as sodium urate crystals (MSU), the selective A1R antagonist DPCPX (8-cyclopentyl-1,3-dipropylxanthine, 8-cyclopent-1, 3-dipropylxanthine), Chloroquine (CQ), Colchicine (Colchicine, Col), available from sigma. MSU was carefully ground and sterilized, formulated with sterile PBS to the desired concentration, shaken vigorously before use; dimethyl sulfoxide (DMSO) was purchased from Amresco; dissolving HEA with a small amount of DMSO, and dissolving with normal saline to obtain the desired concentration; saline (DMSO less than 1%) as solvent control; trizol kit was purchased from life technologies, USA; A1R antibody (ab82477), A2AR (ab 1699756) antibody, LC3(ab48394), p62(ab56416), Caspase-1(ab108362) were purchased from Abcam, and NLRP3 antibody was purchased from Novus.

The statistical method comprises the following steps: each group of data is expressed by mean ± standard error. Statistical analysis is carried out by adopting SPSSl6.0 software, single-factor variance analysis which is completely randomly designed is adopted for comparison among a plurality of sample averages, and Bonferroni test is adopted for pairwise comparison among a plurality of averages.

Example 1 of implementation: preparation of a test sample HEA.

Pulverizing cultured Cordyceps cicadae (Paecilomyces cicadae by solid fermentation or liquid fermentation) into 200 mesh granules (525 g), extracting with 40% and 90% acetic acid for 2 times and 1 time respectively, mixing filtrates, and distributing with dichloromethane (CH2Cl2), Ethyl Acetate (Ethyl Acetate) and n-butanol (n-Butyl Alcohol) to obtain water phase portion CYD. Removing polysaccharide to obtain CYDd, and eluting with CDA-40 type macroporous resin 50% ethanol to obtain CYDd-2. Finally, the CYDd-2A is obtained by Sephedex LH-20 methanol purification, the structure is identified as HEA, and the purity is 98.62%. Example 2 of implementation: the active substance for easing pain of cordyceps cicadae by a twisting method is screened.

The Kunming mice were divided into five groups, which were the solvent control group, CYD group, CYDd-2 group, and CYDd-2A (HEA) group (each component in the extraction process in example 1). The solvent control group is injected with normal saline in the abdominal cavity, the other groups are respectively injected with CYD (crude drug 5g/Kg), CYDd-2 (crude drug 5g/Kg) and CYDd-2A (HEA, crude drug 5g/Kg) in the abdominal cavity, 0.7% acetic acid is injected after 30min of injection, the times of writhing of the white mouse within 10min are recorded, and the inhibition rate of the writhing reaction of pain generated by the 0.7% acetic acid is calculated.

As a result: cordyceps cicadae and analgesic effect of cordyceps cicadae extract

CYD, CYDd-2, and HEA significantly inhibited the writhing of mice after being administered to the abdominal cavity for 30min, and the analgesic inhibition rates were gradually increased to 95.25%, 97.81%, 98.20%, and 99.00%, respectively, which were very significantly different from the control group (see fig. 6, p < 0.01). Therefore, the analgesic active molecule in the cordyceps sobolifera extract is HEA, which further proves that the mixtures obtained from different processes in the cordyceps sobolifera extract can obviously inhibit the mouse writhing, although the different degrees play a role in inhibiting, which is probably the difference of the purity of HEA in different processes and the different effects. However, the only effective substance after final purification was HEA, thus demonstrating that the substance effective in significantly inhibiting the occurrence of mouse writhing was HEA, not other substances in the mixed substance. Of course, HEA can be extracted from cicada fungus and other fungi, and obviously, HEA can also be directly purchased in commerce.

EXAMPLE 3 analgesia of HEA derivatives

HEA is used as a lead, structural modification is carried out on the HEA to obtain a series of derivatives, and a derivative B-H is obtained through analgesic activity screening, wherein the general formula of the compound A, B, C, D, E is shown in formula II, the structural formulas of A-D are respectively R1, CH2CH2OH, CH2CH2CH2OH, C (CH3)2CH2OH and CH (CH3) CH2OH, R2 is H, wherein the compound E, R1, CH (CH3) CH2OH and R2 are NH 2. Wherein the compound A is N6- (2-hydroxyethyl) adenosine (HEA), the general formula of the compound F, G, H is shown in formula III, and R in the structural formula is CH3O, OH and H respectively.

Dividing Kunming mice into ten groups, namely a solvent control group, a morphine hydrochloride group (10mg/Kg), an A (HEA) group, a B group, a C group, a D group, an E group, an F group, a G group and an H group. The solvent control group was intraperitoneally injected with physiological saline, the other groups were intraperitoneally injected with A, B, C, D, E, F, G, H (1mg/Kg), respectively, and after 30min of injection, 0.7% acetic acid was injected, and the number of writhing of mice within 10min was recorded, and the inhibition rate of the writhing reaction to pain generated by 0.7% acetic acid was calculated.

As a result: analgesic effect of HEA and its derivatives

A-H can obviously inhibit the mouse writhing after being administrated in an abdominal cavity for 30min, and is 75.79%, 78.14%, 82.03%, 83.70%, 80.42%, 93.84%, 90.19% and 88.33%, and has very obvious difference compared with a solvent control group (see figure 10, p is less than 0.01).

EXAMPLE 4 rat gout model and Experimental groups

Gout model group: gout modeling was performed by injecting 50 μ LMSU solution (20mg/mL) into the anesthetized rat right ankle joint, inserting a 6-gauge needle into the joint cavity from 45 ° orientation into the tibia on the dorsal side of the rat right ankle joint, with the contralateral bulge of the joint capsule as the standard of injection (Coderre & Wall 1988), to provide a gout model group.

Grouping: in the provided gout model group, 88 male SD rats with the weight of 180-220g are randomly divided into ten groups, wherein the ten groups are respectively (1) and a normal control group containing 1% DMSO physiological saline; (2) a gout model group; (3) 3 treatment groups of HEA (5,7.5,10mg/Kg) administration group; (4) colchicine administration group (Col, 3 mg/Kg); (5) the DPCPX (2mg/Kg) and the HEA (7.5mg/Kg) are combined to form a medicine group; (6), the group consisting of the drug combination of 3-meladenine (3-MA, 15mg/Kg) and HEA (7.5mg/Kg), (7), the chloroquine group of the normal control group, (8), the chloroquine group of the model group, (9), and the chloroquine group of the HEA (7.5mg/Kg) administration group (the treatment of 7.5mg/Kg was found to be the best, and this treatment was selected).

The experimental method comprises the following steps: treatment 5 of DPCPX, treatment 7-9 of chloroquine or treatment 6 of 3-MA intraperitoneal injection for 15min, then the corresponding treatment of HEA administration, and other combinations of simultaneous administration ( treatment 1,3, 4), wherein treatment 2 was given in equal amounts of 1% DMSO saline as a control. After each group of rats was administered in the abdominal cavity for 30min, the rats were anesthetized, and the right ankle joint of the normal control group was injected with 50. mu.L of sterile PBS solution, and the remaining groups were administered with the same amount of MSU solution. Among them, the experiment of the derivatives of B to H is identical to the experiment process of A (the specific data is omitted).

EXAMPLE 5 measurement of swelling degree of rat Joint (Experimental treatment of EXAMPLE 3)

The mark for the ankle joint of the rat is drawn into a circle before gout modeling, the circumference of the mark for the ankle joint of the rat is measured to be the initial joint circumference, the circumference is measured at the marked position after the gout modeling, and the joint swelling rate is calculated. Joint swelling rate ═ initial ankle circumference/initial ankle circumference of point to be measured 100.

As a result: HEA relieving ankle joint swelling degree of rats

As shown in fig. 1, the swelling degree of joint of the model group after molding is obviously increased (p <0.01) and lasts for the whole experimental period. After the positive control colchicine (Col) is applied, the swelling degree is obviously reduced, and the effect lasts from 4 hours to 24 hours. Compared with the model group, the three groups of treatment groups with different concentrations of HEA reduce the swelling degree of the joints to different degrees. Wherein, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

Example 6-rat inflammatory factors TNF- α and IL-1 β assays (experimental treatment with example 3).

After molding for 12h, removing fur from the ankle joint of the rat, washing with 1mL of PBS containing EDTA (3mM) and heparin (25 units/mL), taking the whole blood of the rat, standing at room temperature for 1h, centrifuging at 3000rpm for 10min together with the washing solution, and taking the supernatant for storage at-20 ℃. TNF-alpha and IL-1 beta were assayed according to the protocol of the ELISA kit. HEA (7.5mg/Kg) was the most significant for the group administered, so this concentration was used in the following experiments. The experimental results are as follows: HEA reduces TNF-alpha and IL-1 beta levels in rat serum and joint wash

FIG. 2 shows the expression of TNF- α and IL-1 β in serum, which was significantly higher in the model group (p <0.05, p <0.01) than in the blank control group. Compared with the model group, HEA (5mg/Kg) can reduce the level of IL-1 beta (p <0.05), HEA (7.5mg/Kg) can obviously reduce the levels of TNF-alpha and IL-1 beta (p <0.05, p <0.01), and HEA (10mg/Kg) can reduce the levels of TNF-alpha and IL-1 beta (p < 0.05).

FIG. 3 shows the TNF-alpha and IL-1 beta expression in the joint wash, which was significantly higher (p <0.05, p <0.01) than the blank control group. Compared with the model group, HEA (7.5mg/Kg) obviously reduces the TNF-alpha and IL-1 beta levels (p <0.05), and HEA (10mg/Kg) reduces the TNF-alpha level (p < 0.05). Wherein, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

The experimental results are as follows: effect of HEA on autophagy of arthritic rats in gout

The change in autophagy was demonstrated by comparing the difference in LC3II/LC3I values expressed by chloroquine in the normal, model and administered groups. If one treatment condition is not obviously different from the chloroquine group LC3II/LC3I of the treatment condition, the degradation of autophagy under the treatment condition is inhibited, otherwise, the degradation of autophagy under the treatment condition is normal

As shown in FIG. 5, in the blank control group, the LC3II/LC3I levels were significantly up-regulated (p <0.01) after chloroquine administration, indicating that autophagic degradation was normal in the blank group. The LC3II level was not different between the model group and the model group + chloroquine group, indicating that MSU modeling inhibited autophagic degradation. While the HEA treated group decreased LC3II/LC3I values, and the decreasing effect of HEA on model group LC3II was attenuated by chloroquine. These demonstrate that HEA restores autophagic degradation inhibition by MSU modelling, i.e. HEA promotes autophagy in a gout model. Wherein, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

Example 7-rat A1R, A2AR, p62, LC3, NLRP3, Caspase-1 protein assays (experimental treatment in example 3).

The synovial membranes of each group were washed with PBS and then blotted dry. Approximately 50mg of synovial tissue was homogenized in RIPA lysate (RIPA lysine Buffer) and phenylmethylsulfonyl fluoride (PMSF) tissue lysate, centrifuged, and the supernatant was equilibrated after measuring the protein concentration by BCA method and incubated at 37 ℃ for 30 min. The resulting protein samples were electrophoresed on 10-12% SDS-PAGE gels and transferred to PVDF membranes, which were blocked for 2h in 5% skim milk and used as internal controls (3:8000) with tubulin at 4 ℃ overnight in antibodies A1R (1ug/mL) or A2AR (1:1000) or LC3(1:1000) or p62(1:1000) or caspase-1(1:1000) or NLRP3(1: 1000). After incubation with secondary antibody (1:7000), exposure was carried out.

The experimental results are as follows:

HEA's effect on caspase-1, LC3(LC3 antibody showed two LC3, one LC3II, the other LC3I, generally indicated by LC3II/LC3I values, collectively referred to as LC3), NLRP3, p62 expression.

As shown in figure 4, NLRP3(p <0.01), caspase-1(p <0.05), LC3II (p <0.01) and p62(p <0.01) all rise obviously in synovium of the rat after the model building. And HEA (7.5mg/Kg) injected intraperitoneally can obviously reverse the expression up-regulation (p is less than 0.01) of NLRP3, caspase-1, LC3II and p62 caused by MSU. The HEA can down-regulate the expression level of p62 protein, and the mRNA of p62 has no difference in the transcriptome result compared with that of a model group, which indicates that the HEA can promote the degradation of p62 and thus the expression level of p62 protein is reduced. Wherein, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

Example 8: RNA extraction, library construction and RNA sequencing (experimental treatment with example 3).

After 12 hours of molding for each treatment in example 3, synovial membranes of right ankle joints of rats in the model group and the HEA administration group were taken and rapidly placed in liquid nitrogen for rapid freezing. Synovial membrane total RNA was extracted according to the instructions of Trizol kit (from the latter commercial vendor) and the sample reliability test was performed using a NanoDrop ND-1000 spectrophotometer and Agilent 2100 bioanalyzer. After mRNA was isolated using NEBNext Poly (A) mRNA magnetic isolation module, a cDNA Library was obtained by constructing transcriptome according to NEBNext Ultra RNA Library Prep Kit for Illumina and NEBNext1 Multiplex oligonucleotides for Illumina Kit, each of which was treated for 2 biological replicates. And finally, carrying out high-throughput transcriptome sequencing on the library by using an Illumina HiSeq 2500 sequencing platform.

The gene expression level was estimated by calculating the FPKM (fragments per kinase of exon per fragments mapped) value using the Cufflinks software. Comparing the log values of the ratios of the gene expression levels of the samples treated differently, selecting the log ₂ The absolute value of the ratio is 1 or more, and the difference gene is selected by a False Discovery Rate (FDR) of less than 0.05. The genes were aligned to different protein databases by BLASTX, including Nr, Swiss-Prot, of NCBI, with an E value of 10 ^-5 Is the screening threshold. The function of the differential genes is predicted, classified or annotated by Gene Ontology (GO) and Clusters of Organizational Group (COG) databases, and the difference table is classified or annotated by KEGG databaseAnd performing pathway annotation analysis on the gene expression.

Screening of genes involved in pain modulation in differential genes

The pain database was assembled by reference to the previous method (Ultsch et al.2016) and aligned to 932 difference genes. The Pain related gene Database mainly comprises 531 Genes which are a Pain gene Database (the Pain Genes Database, http:// www.jbldesign.com/jmogil/enter. html), related Genes of human Pain genetic diseases, Genes which appear at least three times in a document of chronic Pain gene screening and Genes of targets of analgesic clinical trials. The 531 genes are all genes which are changed when pain occurs, and a pain gene database is compared with 932 difference genes regulated and controlled by HEA, and the total part is the pain gene regulated and controlled by HEA.

Screening of cordyceps sobolifera analgesic compound analgesic gene

Reference is made to the pain gene bank of LaCroix-Fralish et al (2011). The document summarizes data of 20 mouse pain gene chips from 2002-. All the genes in the gene bank are up-regulated or down-regulated when pain occurs, so that the HEA can express the analgesic effect by reversing the regulation direction of the genes. Pain genes obtained by screening 531 pain genes are compared with the pain gene library and are combined with related documents (Sawynomk 2016; Ferre et al 2007; Cheng et al 2016; Frost et al 2016; Jin et al 2006; Katano et al 2008), and genes with the HEA regulation direction opposite to that shown in the gene library are selected as analgesic genes.

The experimental results are as follows:

HEA regulated pain genes

This transcriptome data has been uploaded at the National Center for Biotechnology Information (NCBI) under the accession number SRP 094913. There are 57 pain genes associated with HEA and its derivatives, as shown in figures 7 and 8, including the table genes below. Wherein, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

Table 1; HEA-associated pain genes

Note: when a comparison of the signatures of the previously discovered pain genes with those associated with expression of HEA and its derivatives was made, 57 pain genes were found associated with HEA and its derivatives, the Csrp3(SEQ ID No.52) and Lxn (SEQ ID No.56) of which were not within 57 pain genes.

The above 59 genes were found to be associated with pain, and in general, if the up-regulated mRNA expression of one gene is painful, the down-regulated mRNA expression has an analgesic effect, and vice versa, and if the down-regulated mRNA expression of one gene is painful, the up-regulated expression has an analgesic effect. Among the 59 genes found in this finding, up-or down-regulated expression of some of the genes still has not been confirmed to be directly related to pain to date, because some of the genes have been shown to have contrary conclusions in different prior literature reports. However, once the functions of these HEA-associated pain genes are determined, the mechanisms of action of HEA in relieving pain or causing pain are determined.

HEA and derivative regulated analgesic gene

Up-regulated the following genes: c1qb (complete Component 1, Q Subcomponent, B Chain), C1qc (complete Component 1, Q Subcomponent, C Chain), Timp1(Tissue inhibitor of metalloproteinase 1), Lum (Lumican), Csrp3(Cysteine and glucose-rich protein 3), Stmn4 (Stahmin-like 4), Uchl1(Ubiquitin C-Terminal Hydrolase L1), Ctsk (Cathepsin K) and Lxn (Latexin) and the following genes to be down-regulated: nsf (N-ethyl meleimide sensitive fusion protein), Adora1 and Adora2a have all been demonstrated to be painful by prior literature. If the opposite expression occurs for the above genes, analgesia is expressed.

According to the invention, through comparative analysis, HEA and derivatives thereof are found to be capable of regulating the down-regulation of c1qb, c1qc, Timp1, Lum, Csrp3, Stmn4, Uchl1, Ctsk and Lxn genes and regulating the up-regulation of Nsf, Adora1 and Adora2a genes, so that the HEA can play an analgesic role (FIGS. 7-8).

Western blot validation of A1R (Adora1) and A2AR (Adora2a)

The expression conditions of A1R and A2AR in synovial membranes of joints of rats with gout are detected by using a Western blot method, and the results are shown in figure 9. Model groups A1R and A2AR were elevated from the blank control group and are shown. Compared with the blank group, the HEA (7.5mg/Kg) administration group has a significant increase of A1R (P <0.01) and a significant decrease of A2AR (P < 0.01). Compared with the model group, A1R is also increased (P <0.05), and A2AR is obviously decreased (P < 0.01). After DPCPX (2mg/Kg) and HEA (7.5mg/Kg) are simultaneously applied, the expression of A1R protein is obviously reduced, and no statistical difference is generated compared with a blank group and a model group. The results of protein expression indicate that the analgesic effect of HEA is associated with up-regulation of A1R and down-regulation of A2 AR. Among them, the experimental results of the derivatives of B-H are similar to those of A (the specific data are omitted).

The invention shown and described herein may be practiced in the absence of any element or elements, limitation or limitations that are specifically disclosed herein. The terms and expressions which have been employed are used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, and it is recognized that various modifications are possible within the scope of the invention. Thus, it should be understood that although the present invention has been specifically disclosed by various embodiments and optional features, modification and variation of the concepts herein described may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this invention as defined by the appended claims.

The contents of the articles, patents, patent applications, and all other documents and electronically available information described or cited herein are hereby incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference. Applicants reserve the right to incorporate into this application any and all materials and information from any such articles, patents, patent applications, or other documents.

Organization Applicant

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Country :

PostalCode :

PhoneNumber :

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<110> organic autumn name

Application Project

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Application of <120> Title, N6- (2-hydroxyethyl) adenosine and derivatives thereof in preparation of medicines for treating gout

<130> AppFileReference :

<140> CurrentAppNumber :

<141> CurrentFilingDate : - -

Sequence

--------

<213> OrganismName : Csf2rb

<400> PreSequenceString :

atggaccagc acatggcatt tatctggggg ctgtgctaca tggccctggt ggctctctgc 60

tggagacacg gggtgacaaa ggcagaagaa actgtccctc tgaagactct gcagtgctac 120

aacgactata tcgagcgcat catctgcagc tgggccgaca cggaggacgc ccaggggctc 180

gttaacctga ccctctatca ctggctagac aagaaacagc caatgtcctg tgagctcagt 240

gaggacctca tgtggtcaga gtgcccgtca tcccaccgct gtgtgcctag aagatgtgtc 300

ctcccctata cacagttttc cgtctcaaaa gaagactact actcactcca gccggatcgt 360

gatctgagta tccatctcgt ggttccgctg gcccagcatg tgcagccacc acctcccaag 420

gacatcagca tcagcccctc tggggaccat ttcctgctga agtggagtgt gccccatggg 480

gatgcccagg tctctctgct ttcacaaaag gacatacagt ttgaggtggc ttataagcgg 540

cttcaggact cctgggagga tgcctccagc ctccacacta gcaacctctg ggtcactctt 600

gagccaaagc tcttcctacc caacagtatc tatgtggccc gcgtgcgcac tcagctagcc 660

ccaggttcga gcttgtccgg aagacctagc agatggagcc cagaggtaca ctgggactct 720

ccgacagggg acaaggcccg gccacagaac cttcagtgct tctttgatgg gatccagtcc 780

ctcaactgct cctgggaggt gtggaccaag gtgactgact ctgtttcctt tgggctcttc 840

tatagctcca gtcccaaagc tggggagaag aaatgctctc cagtggtgaa ggagctgcag 900

gccagccgct acacccggta ccactgcagc ctaaatgtgt ccgaccccgc tgcacacagc 960

cagtacaccg tctctgttaa gcggctggaa caagggaagt tcatcgagag ctttaaccac 1020

atccagatga atcctccaac cctcaacctg accaagaaca gagacagcta cagcctgcat 1080

tgggagactc agaaaatgtc ctatccattc atccagcatg cattccaggt ccagtacaag 1140

aagaaactgg acagctggga ggacagcaag acagagaacc taaatcatgc ccacagcatg 1200

gacctgccac agctggagcc tggcacctca tactgcgcca gggtgagggt caagaccatc 1260

cctgaataca aagggctctg gagcgagtgg agcaatgagt gcacctggac gactgactgg 1320

gtgatgccca cattgtggat agtcctcatc ctggtcttcc tcatcctcac cttcctcctg 1380

gccctccgct ttggctgcat ctatgggtgc aagttgtaca gaaaatggaa ggagaaaatc 1440

cccaacccca gcaagagcct ccttttccag gatggaggta aaggactctg gcctcctggc 1500

agcacggtga ccttctccag taagaacccc actccccagg ggccacagag caaccttttc 1560

tctgagctac agggggtgtc atatacacat ctggaggaca acgaagtgtc acctctcacc 1620

atagaggacc ccaacattat tcgagatcca tcatctggcc ctgatacaac cccagctgcc 1680

tcatctgaac ccatggagca gtcttccaat gttcaagtag acccaccaac tctttctggc 1740

agacccagga agcaattacc cagctttgac ttcaatggcc cgtacctggg gcctccccaa 1800

tcccactccc tgcccgatct cccaggccag ctggtttccc cccagggggg tggaagcctg 1860

aagccagcac tgccaggctc cttggagtac atgtgtctgc cccctggagg gcaagtgcaa 1920

ctggtcccac tgtcccaggt gatggggcag ggccgggatg tggatgtgca gtgtgggtcc 1980

agcctggaga ccacagagag cccttccatg gaatcaaggg agagccctcc agttgagctg 2040

aaggaggagg aacaggagcc aagggacaac ccagtgactc tccccataag ctctggaggc 2100

cccaaagaca gtaaggtggc ctctgagtat gtcacacctg cagatctggt gctcactctg 2160

cccacagagc ccttgtctac ctctctgggt ccctctctag aggtctcctc agcccaaagc 2220

cccagtctct gtctcaagct gcccagggtc ccttctgaaa gtccagctct tgcaccagca 2280

gtgtttgaag attacgtgga gctgcctcca agcatgagcc agcctgccaa gtctcttcca 2340

ggcaatcctg ctcctcctgg gccaagcagc cccgcagtga gcccaggaga gcccagggag 2400

gaagtaggcg cagcccccac acaccctgaa ggcctccttg ttcttcagca ggttggggac 2460

tactgcttcc tccctggcct gggacccagc cccctctcac cacccagtaa gccaccctct 2520

ccgggcctgt gtctcgagac tgaggaccta gacaaggact tgtctgtcaa aaagcttccc 2580

taccaaccca taccccaggt gccagccatt cagtttttca agtccctaaa gcatcaggac 2640

tacctgtcac tgcccccttg ggacaatagc cagcctggga aggtgtgctg a 2691

<212> Type : DNA

<211> Length : 2691

SequenceName : 1

SequenceDescription :

Sequence

--------

<213> OrganismName : Ccl7

<400> PreSequenceString :

atgcagatct ctgccgcgct tctgtgtgtg ctgctcacag ctgctgcttt caccgtgcac 60

gtgtgggccc aaccagatgg gaccaattca tccacttgct gctatgtcaa gaaacaaaag 120

attcccaaga ggaatctcaa gagttacaga aagatcacca gtagtcggtg tccctgggaa 180

gctgttatct tcaagaccaa aaagggcatg gaagtctgtg ctgaagccca tcagaagtgg 240

gttgaggagg ccatagcata cttagacatg aaaacttcaa ctccaaagcc ctga 294

<212> Type : DNA

<211> Length : 294

SequenceName : 2

SequenceDescription :

Sequence

--------

<213> OrganismName : C4a

<400> PreSequenceString :

atgcggctcc tctggggcct ggcctgggca ttcagcttct tcgcctcctc tctgcagaag 60

cccaggttgc tcctgttctc cccttctgtg gttaatttgg ggacccccct gtcagtaggg 120

gtgcagctcc tggatgcccc tgcaggacag gaggtgaaag gctcagtgta cctcagaaac 180

ccaacaagcg gtccctgctc cccaaagaag gactttaagc tcagctcggg aaatgacttt 240

gtgcttctca gacttgaggt cccactgaaa gatgtgagga gttgtggcct ctttggcctg 300

cgcagagccc cccacatcca gctggtggct cactctccgt ggctaaagaa cacagcatcc 360

aaagccaccg agactcaggg ggtcaacctg cttttctctt cccgacgagg ccacatcttt 420

gtgcagaccg atcagcctat ctataaccct gggcagcggg ttcgttatcg ggtctttgca 480

ctggatcaaa agatgcgtcc atctactgac accctcacgg tcacagtgga gaactcccat 540

ggcctccgtg tacgcaagaa ggaggtattt gcccccacat ccatcttcca agatgacttc 600

ataattccag acatctcaga gccggggacc tggaagatct cagctaggtt ctcagatggc 660

ctcgagtcca acagaagcac ccacttcgaa gtgaagaaat atgtccttcc caactttgag 720

gtgaagctta ctccttggaa gccatatatc ctgacagtgc ctagctaccg tgaagaaata 780

caattagacg tccaggccag atacgtctac gggaagcccg tgcagggcgt ggcatatacc 840

cgcttcgcac tcatggatga gcaggggaag aagtctttcc tccggggcct agagacccag 900

accaagttgg tggaaggcca gactcacatc tccatctcaa gggaccagtt ccaggctgcc 960

ctgggtaaag tgaatactga gatcggagac ctggaggggc tgcggctcta cgctgccgta 1020

gctgtcattg agtctccagg aggagagatg gaggaggcgg agctcacatc ctggcccttc 1080

gtgtcatctg ccttctcctt ggatctcagc cacaccaagc agcatcttgt gcccggggcc 1140

cccttcctgc tgcaggccct ggttcgagaa atgtcaggct ctgaagcctc tgatgttcct 1200

gtcaaagtct ctgccacatt gttgtctggg tccgactcga aagtccttga ctttcaacag 1260

aacaccaatg ggattggcca agtcagcttt tccatccatg tcccgccaac catcaccgaa 1320

cttcgactct tggtatctgc cggttccctc tacccagctg tagccaagct caccgtgcaa 1380

gccccacctt caagaggccc cggctttctg tccattgagc cattagacct tcggtcccct 1440

cgtgtggggg acaccttcgt cctgagcctt cgaaccgtgg gcatccctat gcctaccttc 1500

tctcattact actacatgat catctccaga ggccagatca tggccatgag tcgggagccc 1560

aggagggccc tgacctccat ctctgtcttg gtggaccatc acctggctcc ctctttctac 1620

tttgtggcct acttctatca ccaaggactc ccagtggcca actccctgct catcaacgtc 1680

cagcccgggg actgtgaggg caagctggaa ttgaaggtgg atggtgccaa ggagtatcgc 1740

aatggagaca gcatgaagct ccaacttcaa actgactctg aagccctggt ggcactggga 1800

gctgtggaca cagctctgta tgctgtgggt ggccggtctc acaaacccct cgacatgagc 1860

aaggtctttg aagtaatgaa cagctacaac cttggctgtg gtcccggagg cggggacgat 1920

gcccttcagg tgttccagac tgctggtctg gccttttctg acggtgatcg actaactcag 1980

accaaagaga acctgagctg tcccaaggag aagaaaagtc ggcaaaagcg aaatgttaac 2040

ttccagaagg ctattagcga gaagttgggc cagtattcct ctccagacac caagcgctgc 2100

tgccaggatg ggatgaccaa gctgcccatg gcacgcacct gcgagcagag ggcagcccgc 2160

gtgcctcagc cagcctgccg ggagcccttc ttgtcctgtt gcaagtttgc tgaggacctc 2220

cgcaggaacc agaccaggag ccaggcgggc ctcgcccgag cccaggacat gctgcaggag 2280

gaggatctga tagatgaaga tgacattctc gtgcgcagct tcttcccaga gaactggctc 2340

tggagagtgg aacctgtgga ccgctccaaa ctgttgactg tgtggctccc tgactctctg 2400

accacatggg agattcatgg tgtgagcctg tccaaaagca aaggcttgtg tgtagccaag 2460

ccaactcgcg tgcgagtgtt ccgagaattc cacctgcacc tgcgcctgcc catctctgtc 2520

cggcgcttcg agcagcttga attacggccc gttctctaca actatctgag tgaggacgtg 2580

actgtaagtg tccatgtgtc cccagtggag gggctgtgcc tggctggtgg tggattgctg 2640

gcccagcaag tatcagtgcc tgcaggctcc gcccggcctg tggccttctc tgtggtaccc 2700

acagctgctg ccagcatacc cctgaaagtg gtggctcggg ggtcttttac tataggggat 2760

gctgtgtcta agattctcca aattgagaag gaaggggcca tccacagaga agagatagtc 2820

tacaacctcg accccctaaa taaccttggt cggagtttgg aaatccctgg cagctcagac 2880

cccaacgtca ttcctgatgg agatttcagc agcttcgtca gggttacagc ctccgaaccc 2940

ttggagactt tgggctccga aggcgccttg tccccaggcg gagtggcctc cctcctgagg 3000

ctccctagag gctgtgcaga acaaaccatg atctatttgg cccctaccct gactgcttcc 3060

cactacctgg acaggacgga gcagtggagc aaactgcccc ctgagacaaa ggaccatgct 3120

gtggatctga ttcagaaagg ccacatgcgg atccagcagt ttcggaagaa agatggctcc 3180

tttggggcat ggttacaccg ggacagcagc acctggctca cggcttttgt gctgaagatt 3240

ctgagtctgg cccaggaaca gattggtgac tcccccgaga agctgcaaga gaccgctggg 3300

tggctgctgg gccagcagct ggacgatggc tcattccacg acccgtgtcc agtcatccac 3360

agaggcatgc aggggggctt ggtgggaacc gatgagaccg tggcactgac cgcctttgtg 3420

gtcatcgccc ttcaccacgg gctggccgtc ttccaggacg agaactcaca gcagctgaag 3480

aagagggtgg aagcctccat cacgaaggcg aactcgttct tggggcagaa ggcaagtgct 3540

gggctcctgg gtgcacacgc atccgccatc acagcctatg ccctgacact gaccaaggcc 3600

tcggaggacc tgcggaatgt tgcccacaac agcctgatgg ccatggctga ggaaacaggg 3660

gaaaacctct actggggctc agccattggc tctcaggaca acgttgtgtc gtccacccca 3720

gcccctcgta atccctcaga acctgtgccc caggccccag ccctgtggat cgaaaccaca 3780

gcctacggcc tgctacatct gctgctgcgg gaggggaagg gagaaatggc tgacaaggtg 3840

gcaacctggc tcacacacca gggaagcttc caagggggat tccgcagtac ccaggacact 3900

gtggtcaccc tggatgccct gtctgcatac tggatcgctt cacacaccac cgaggagaag 3960

gcactcaatg tgactctcag ctccatgggc cgcaatgggt acaagtccca cctgctacag 4020

ctgaacaacc accaagtcaa gggcctggag gaggagctga agttctccct gggcagcacc 4080

atcaatgtga aagtgggagg aaacagcaaa ggcaccttaa agatccttcg tacctacaac 4140

gtcctggaca tgaagaacac aacatgccaa gaccttcgga tagaagtgac ggtcacaggc 4200

tatgtggaat atacaaggga ggccaacgaa gactatgaat acgactatga tatgccagcc 4260

gcagatgacc ccagtgtcca ctcgcagcct gtgacacccc tgcagctgtt tgagggtcgt 4320

cggagccgcc gcaggaggga ggcccccaag gctgcggatg agcaggagtc cagagtgcag 4380

tacaccgtgt gcatctggcg gaacggtaac ctggggctgt ctgggatggc cattgctgac 4440

atcaccctcc tgagtggatt ccaagccctg agggccgacc tggagaagct gacctccctc 4500

tctgaccgtt acgtgagtca ctttgagacc gacgggcccc acgtcctact atactttgac 4560

tcggtcccga ccacccggga gtgtgtgggc tttggagcct tgcaggaggt ggccgtgggg 4620

ctggtgcagc cagccagtgc tgtccta 4647

<212> Type : DNA

<211> Length : 4647

SequenceName : 3

SequenceDescription :

Sequence

--------

<213> OrganismName : Arpc1b

<400> PreSequenceString :

atggcttacc acagcttcct ggtggaaccc atcagctgcc atgcctggaa caaggaccgt 60

actcagatcg ccatctgccc caacaaccac gaggtgcaca tctacgagaa gagcggtgcc 120

aagtggaaca aggtgcacga gctcaaggag cacaacgggc aggtgacagg catcgactgg 180

gcccccgaga gtaaccgcat tgtgacctgc ggcacagacc gcaatgccta tgtgtggacg 240

ctgaagggcc gcacgtggaa gcccacgctg gtcatccttc ggattaatcg agctgcccgc 300

tgcgtgcgct gggcccccaa tgagaacaag ttcgccgtgg gcagtggctc ccgtgtcatt 360

tccatctgtt attttgagca ggagaatgac tggtgggtgt gcaaacacat caaaaagccc 420

atccgctcca ctgtccttag cctggactgg caccccaaca acgtgctcct ggctgcaggc 480

tcctgtgact tcaagtgcag gatcttctct gcctatatca aggaggtgga ggaacggcca 540

gcccctacac cgtggggctc caagatgccc tttggggagc tgatgtttga atcgagcagc 600

agctgtggct gggtgcatgg tgtctgcttc tcggccggtg ggagccgagt tgcttgggtc 660

agccatgaca gcactgtgtg cctggtagat gctgagaaga agatggccgt ggcaaccctg 720

gcctctgaga cattaccgct cctggccatc accttcatca cagaaaatag tctcgtggca 780

gcgggccacg actgcttccc ggtgctgttt acctatgaca acgctgcggg gacattgagc 840

tttggtggcc ggctggatgt gcccaagcag aactcccagc gtggcctgac agcccgagag 900

cgcttccaga acctcgacaa gaaggccagc tctgaagggg gtgcagccac aggggctggc 960

ctggattcac tgcacaagaa cagcgtcagt caaatctcgg tgctcagcgg gggcaaggcc 1020

aagtgctcgc agttctgcac cacaggcatg gacggtggca tgagcatctg ggatgtgaag 1080

agcttggagt cagccttgaa ggacctgaag atcagatga 1119

<212> Type : DNA

<211> Length : 1119

SequenceName : 4

SequenceDescription :

Sequence

--------

<213> OrganismName : Adamts5

<400> PreSequenceString :

atgcggctcg agtgggcgtc cttgctgctg ctactgctgc tgctgtgcgc gtcctgcctg 60

gccctggccg ctgacaaccc tgccgcggca cctgcccagg ataaaaccag gcagcctcgg 120

gctgctgcag cggctgccca gcccgaccag cggcagtggg aggaaacaca ggagcggggc 180

catccgcaac ccttggccag gcagcgcagg agcagcgggc tggtgcagaa tatagaccaa 240

ctctactctg gcggtggcaa agtgggctac cttgtctacg cgggcggccg gaggttcctg 300

ctggacctgg agagggatga cacagtgggt gctgctggtg gcatcgttac tgcaggaggg 360

ctgagcgcat cctctggcca caggggtcac tgcttctaca gaggcactgt ggacggcagc 420

cctcgatccc tagctgtctt tgacctctgt gggggtctcg atggcttctt cgcagtcaag 480

catgcgcgct acactctgaa gccgctcttg cgtgggtcct gggcagagtc cgaacgagtt 540

tacggggatg ggtcttcacg catcctgcat gtctacaccc gcgagggctt cagcttcgag 600

gccctgccgc cacgcaccag ttgcgagact ccagcgtccc cgtctggggc ccaagagagc 660

ccctcggtgc acagtagttc taggcgacgc acagaactgg caccgcagct gctggaccat 720

tcagctttct cgccagctgg gaacgcggga cctcagacgt ggtggaggcg gaggcgccgt 780

tccatctcca gggcccgcca ggtggagctc ctcttggtgg ctgactcttc catggccaag 840

atgtatgggc ggggcctgca gcattacctg ctgaccctgg cctctattgc caaccggctg 900

tacagtcatg caagcatcga gaaccacatc cgcctggccg tagtgaaagt ggtggtgctg 960

accgacaaga gtctggaggt gagcaagaac gcggccacga ccctcaagaa cttttgcaaa 1020

tggcagcacc aacacaacca gctaggtgat gaccatgagg agcactacga tgcagccatc 1080

ctgttcacca gagaggattt atgtgggcat cattcatgtg acaccctggg aatggcagac 1140

gttgggacca tatgttctcc ggagcgcagc tgcgctgtga ttgaagatga tggcctccat 1200

gcagctttca ctgtggctca cgaaattgga catctacttg gcctctctca cgacgattcc 1260

aaattctgtg aagagaactt tggttctaca gaagacaagc gtttaatgtc ttcaatcctt 1320

accagcattg atgcatccaa gccctggtcc aaatgcactt cagccacgat cacagaattt 1380

ctggatgacg gtcatggtaa ctgtttacta gatgtaccac ggaagcagat tctgggcccc 1440

gaggaactcc caggacagac ctatgatgcc acccagcagt gcaacttgac atttgggcct 1500

gaatattctg tgtgccctgg catggatgtc tgtgcacggc tgtggtgtgc tgtggtgcgc 1560

caaggccaaa tggtgtgtct gaccaagaag ttgcctgccg tggagggcac tccctgtggg 1620

aaaggaagaa tctgcctgca aggcaaatgt gtggacaaaa ctaagaaaaa atattactcg 1680

acatcaagcc atggaaattg ggggtcctgg ggcccctggg gtcagtgttc tcgctcttgc 1740

gggggaggag tacagtttgc ctaccgccat tgcaataacc ccgcacctcg aaacagtggc 1800

cgctactgca cagggaagag ggccatatac cgttcctgca gtgtcatacc ctgcccacct 1860

aacggcaaat ctttccgcca cgagcagtgt gaagccaaaa atggctatca gtccgatgca 1920

aaaggagtca aaacatttgt agaatgggtt cccaaatacg caggtgtcct gccggcagac 1980

gtgtgcaagc ttacgtgcag agctaagggc actggctatt acgtggtctt ttctccaaag 2040

gttacagatg ggacagaatg tagaccctac agcaactccg tgtgtgtccg agggaggtgc 2100

gtgagaacgg ggtgtgacgg catcatcggc tcaaagctac agtatgacaa gtgtggagtg 2160

tgtggagggg ataactccag ttgtacaaag attattggaa ccttcaataa aaaaagcaag 2220

ggttatactg acgttgtgag gatccctgaa ggagcaaccc acataaaagt ccgacagttc 2280

aaagccaaag accagactag attcactgct tacttagccc taaagaagaa aactggcgag 2340

taccttatca acggcaagta catgatctcc acttcagaga ccatcatcga catcaatggt 2400

accgtcatga actacagtgg gtggagtcac agagatgatt ttttacatgg gatgggctat 2460

tcagccacaa aggaaattct gattgtgcag atccttgcaa cagacccaac taaagcatta 2520

gacgtccgtt acagcttttt tgttcccaag aagaccactc aaaaagtgaa ttccgtcatt 2580

agccacagca gcaacaaagt gggactacac tccccgcagc tacagtgggt gacaggcccc 2640

tggctggcct gctctaggac ctgtgacaca ggctggcaca ccaggaccgt gcagtgccag 2700

gatggaaaca ggaaattagc taagggatgc attctctctc agaggccttc tgcgtttaag 2760

caatgtttgc tcaagaaatg ttag 2784

<212> Type : DNA

<211> Length : 2784

SequenceName : 5

SequenceDescription :

Sequence

--------

<213> OrganismName : Adcy5

<400> PreSequenceString :

atgtccggct ccaaaagcgt gagccccccg ggctacgctg cacagacagc agcgtcgccc 60

gcgcccgggg gaggcccgga gcatcgcgcc gcgtggggag aagccgattc ccgcgccaat 120

ggctaccccc acgcccccgg gggatcgacc cgcggctcca ccaagcgatc tgggggagcg 180

gtgaccccac aacagcagca gcgcctggct agccgttggc gcggtggcga cgacgatgaa 240

gaccctccac taagcggtga cgaccctctg gtcggaggct tcggcttcag cttccgttct 300

aagtcggcct ggcaggagcg cggcggcgac gacggcggtc gcggcagcag gcggcagcgg 360

cggggcgcgg ctggaggggg cagcacccgg gcgccccctg cgggcggcag tggcagttcg 420

gcggcggccg cagcagccgc aggtggcacg gaggtgcgcc cccgctcggt ggaggtgggc 480

ctggaggagc gtcgaggaaa aggccgagcg gccgaggagc tggagcccgg aactggcacc 540

gtcgaggatg gagacgggtc cgaggatgga ggcagttccg tggcgtcagg ctctgggacc 600

ggcacggtgc tgtcgttggg cgcctgctgc ctggccttgc tgcagatatt ccgctctaag 660

aagttcccgt cggacaaact ggagcgtctg taccagcgct atttcttccg cctgaaccag 720

agcagcctca ccatgctcat ggccgtgctg gtgctcgtgt gcctggtcat gctcgctttc 780

cacgcggcac ggcccccgct ccaggtagtc tacctggccg tgttggcagc tgctgtgggc 840

gtgatcctta tcatggctgt gctctgcaac cgtgcagcct tccaccagga ccacatgggc 900

ctggcctgct atgcgctcat tgcagtggtg ctggccgtcc aggtagtggg cctgttgctg 960

ccacagccac gcagcgcctc cgagggcatc tggtggaccg tgttcttcat ctataccatc 1020

tacaccctgc tgcctgtgcg catgagggct gcggtgctca gcggggtgct tctgtcggct 1080

ctccacttgg ccatctctct gcacaccaac gcccaggacc agtttctgct gaaacagctt 1140

gtctccaacg tcctcatctt ctcttgcacc aacatcgtgg gtgtgtgcac tcactaccca 1200

gccgaggtct cccagagaca ggccttccag gagacccggg agtgcatcca agctcggctc 1260

cactcacagc gggagaacca gcaacaggag cgtctcctgc tgtctgtcct tccccgtcat 1320

gttgccatgg agatgaaagc agacatcaac gccaaacagg aggatatgat gttccacaag 1380

atttacatcc agaaacatga caatgtgagc atcctgtttg ctgacatcga aggcttcact 1440

agcctggcat cccagtgtac tgcccaagaa ctggtcatga ccctcaacga gctcttcgcc 1500

cgctttgaca agttggctgc ggagaatcac tgcttacgga ttaagatcct cggggattgt 1560

tactactgtg tctcggggct gcctgaagcc agagctgacc acgcccactg ctgcgtagag 1620

atgggaatgg acatgatcga ggccatctcg ttggtccggg aggtgacagg ggtgaacgtg 1680

aacatgcgtg tgggaattca cagcgggaga gtacactgcg gtgtccttgg cctcagaaag 1740

tggcaattcg acgtgtggtc taacgatgtc acactggcca accacatgga agctggcggc 1800

aaggcaggcc gcatccacat caccaaggcc acactcaact acctgaacgg ggactatgag 1860

gtggagccag gctgtggtgg tgagcgcaat gcctacctca aggagcacag catcgagacc 1920

ttcctcatcc tgcgctgtac ccagaagcgg aaagaagaga aggccatgat cgccaagatg 1980

aaccgccaga gaaccaactc cattggacac aatccgcctc actggggagc tgagcgcccc 2040

ttctacaacc acttgggtgg caaccaggtg tccaaggaga tgaagaggat gggctttgag 2100

gaccccaagg acaagaatgc ccaggaaagt gccaaccctg aggatgaagt ggacgagttt 2160

ctgggtcgag ccatcgatgc caggagtatt gacagactgc gatccgaaca cgtccgaaag 2220

ttcctcttga cctttaggga gcccgactta gagaagaagt actccaagca ggtggatgac 2280

cgatttggtg cctatgtggc ctgtgcctcg cttgttttcc tcttcatctg ctttgtccag 2340

atcaccattg tgccccactc cctgttcatg ctgagcttct acctgtcgtg tttcctgcta 2400

ctggccttgg tggtgtttat atctgtgatc tacgcctgtg tgaagctctt ccctactccc 2460

ctgcagacac tctccaggaa gatagtgcga tccaagaaga acagcaccct ggtcggggtg 2520

ttcaccatca ccctggtgtt cctctcggct tttgtcaaca tgttcatgtg caactctaag 2580

aacctggtgg gttgcctggc agaggagcac aacatcacgg tgaaccaggt gaacgcatgt 2640

catgtgatgg agtcggcctt caactacagc ctgggcgacg agcagggctt ctgtggcagc 2700

ccccagtcca actgcaactt cccagagtac ttcacctaca gcgtgctgct cagcctgctg 2760

gcctgctccg tgttcctgca gatcagctgc atcgggaagc tggtgctcat gctggccatc 2820

gagctcatct acgtgctcat cgtagaggtg cccggtgtca cactctttga caacgctgac 2880

cttctggtca ccgccaatgc catagacttc agcaacaacg ggacctccca gtgccctgag 2940

catgcgacca aggtggcgct gaaggtggtg acgcccatca tcatctctgt cttcgtgctg 3000

gctctgtatc tgcatgctca gcaggtggaa tccactgctc gccttgactt cctctggaaa 3060

ctgcaggcca cagaagagaa ggaggagatg gaggagctgc aggcctacaa ccggcgcttg 3120

ctgcacaaca ttctgcccaa ggacgtggct gcccacttcc tggcccggga gcggcgcaat 3180

gatgaactgt actaccaatc ctgcgagtgc gtggctgtca tgtttgcctc catcgccaac 3240

ttctccgaat tctacgtgga gctagaggcc aacaatgagg gcgttgaatg cctacggctg 3300

ctcaatgaga tcatcgcaga ctttgatgag atcatcagtg aggatcggtt caggcagctg 3360

gaaaagatca agaccatagg tagcacctac atggctgcct ctggcctcaa cgactccacc 3420

tatgacaagg caggcaagac ccacatcaag gctcttgcag acttcgccat gaagctgatg 3480

gaccaaatga agtacatcaa tgagcactcc ttcaacaact tccagatgaa gatcgggctt 3540

aacattggac ctgtagtggc tggggtcatt ggggctcgca agcctcagta tgacatctgg 3600

ggcaatacag taaatgtggc cagccgtatg gacagcactg gggtgcctga ccgcatccag 3660

gttactacag atatgtacca ggtgctggcc gccaacacat accagctgga gtgccggggt 3720

gtggtcaagg tcaagggcaa gggtgagatg atgacctact tcctcaatgg agggcctccc 3780

ctcagttag 3789

<212> Type : DNA

<211> Length : 3789

SequenceName : 6

SequenceDescription :

Sequence

--------

<213> OrganismName : Ptgs2

<400> PreSequenceString :

atgctcttcc gagctgtgct gctctgcgct gccctggcgc tcagccatgc agcaaatcct 60

tgctgttcca acccatgtca aaaccgtggt gaatgtatga gcataggatt tgaccaatat 120

aaatgtgact gtacccggac tggattctac ggtgaaaact gtactacgcc tgagtttctg 180

acaagaatca aattactgct gaagcccacc ccaaacacag tacactacat cctgacccac 240

ttcaagggag tctggaacat tgtgaacaac attcccttcc ttcggaattc aatcatgaga 300

tacgtgttga cgtccagatc acatttgatt gacagcccac caacttacaa tgtgcactac 360

ggttacaaaa gttgggaagc tttctccaac ctctcctact acaccagggc ccttcctcct 420

gtggctgatg actgcccaac tcccatgggt gtgaaaggaa ataaggaact tcctgattca 480

aaagaagttc tggaaaaggt tcttctgagg agagagttca tcccggatcc ccaaggcaca 540

aatatgatgt tcgcattctt tgcccagcac ttcactcatc agtttttcaa gacagatcag 600

aagcgaggac ctgggttcac ccgaggactg ggccatggag tggacttaaa tcatgtttac 660

ggtgaaactc tagacagaca acataaactt cgacttttcc aggatggaaa attgaaatat 720

caggtcatcg gtggagaggt gtatcctccc acagtcaaag acactcaggt agacatgatc 780

taccctcccc acgtccctga gcacctgcgg ttcgctgtgg ggcaggaagt ctttggtctg 840

gtgccgggtc tgatgatgta tgctaccatc tggcttcggg agcacaacag agtgtgtgat 900

atactcaaac aggagcatcc tgagtgggat gacgagcgac tgttccaaac cagcaggctc 960

atactgatag gagagacgat caagatagtg atcgaagact acgtgcaaca cctgagcggt 1020

taccacttca aactcaagtt cgacccagag ctgcttttca accagcagtt ccagtatcag 1080

aaccgcattg cctctgaatt caacacactc tatcactggc atccgctgct gccggacacc 1140

ttcaacattg aagaccagga gtacactttc aaacagtttc tctacaacaa ctccatcctc 1200

cttgaacacg gacttgctca ctttgttgag tcattcacca gacagattgc tggccgggtt 1260

gctgggggaa ggaatgttcc aatcgctgta caagcagtgg caaaggcctc cattgaccag 1320

agcagagaga tgaaatacca gtctctcaat gagtaccgca aacgcttctc cctgaaacct 1380

tacacatcgt ttgaagaact tacaggagag aaagaaatgg ctgcagagtt gaaagccctc 1440

taccatgaca tcgatgccat ggaactgtat cccgccctgc tggtggaaaa gcctcgtcca 1500

gatgctatct ttggggagac catggtagaa cttggagctc cattctcctt gaaaggcctt 1560

atgggtaatc ccatctgttc tcctcaatac tggaaaccta gcaccttcgg aggagaagtg 1620

ggttttagga tcatcaacac tgcctcaatt cagtctctca tctgcaataa tgtgaaaggg 1680

tgtccctttg cctctttcaa tgtgcaagac ccgcagccta ccaagacagc caccatcaac 1740

gcaagtgcct cccactccag actagatgac attaacccta cagtactaat caaaaggcgt 1800

tcaactgagc tgtaa 1815

<212> Type : DNA

<211> Length : 1815

SequenceName : 7

SequenceDescription :

Sequence

--------

<213> OrganismName : Kcnk2

<400> PreSequenceString :

atgcttgcca gcgcctcgcg ggagagaccc ggctataccg caggagtggc ggcccctgac 60

ttgctggatc ccaagtctgc tgctcagaac tccaaaccga ggctctcgtt ctccgcaaaa 120

cccaccgtgc ttgcttcccg ggtggagagt gactcggcca ttaatgttat gaaatggaag 180

acggtctcca cgattttcct ggtggtcgtc ctctacctga tcatcggagc cacggtgttc 240

aaggcgttgg agcagcctca ggagatttct cagaggacca ccattgtgat ccagaaacag 300

aacttcatag cccagcatgc ctgcgtcaac tccaccgagc tggatgaact catccagcaa 360

atagtgacgg ccataaatgc agggattatc cccttaggaa acaactccaa tcaagttagt 420

cactgggacc tcggaagctc tttcttcttt gccggcactg ttatcacaac cataggattt 480

ggaaacatct ccccacgaac tgaaggtgga aaaatattct gtatcatcta tgccttgctg 540

ggaattcccc tctttggttt tctactggct ggggttgggg atcagcttgg aaccatattt 600

ggaaaaggaa ttgccaaagt ggaggacaca tttattaagt ggaatgttag tcagaccaag 660

attcgtatca tctcgaccat catcttcatc ctgtttggct gtgtcctctt cgtggctctc 720

cccgccgtca tattcaagca catagaaggc tggagtgccc tggacgccat ctactttgtg 780

gtcatcactc tgaccaccat tggatttggc gattatgtgg caggtgggtc ggacattgaa 840

tatctggact tctacaagcc cgttgtgtgg ttctggatcc tcgttgggct ggcctacttt 900

gcggctgttc tgagcatgat tggagactgg ctacgggtga tatctaagaa gacgaaggaa 960

gaggtgggag agttcagagc gcatgccgct gagtggacag ccaatgtcac agccgagttc 1020

aaggaaacaa ggaggcggct gagtgtggag atctatgaca agttccagcg tgccacgtcc 1080

gtgaagcgga agctctctgc agagctggcg ggtaaccata accaggaact gactccatgt 1140

aggaggaccc tgtcggtgaa ccacctgacc agcgagaggg aagtcctgcc tcccttgctg 1200

aaggctgaga gcatctatct gaacggtctg acaccacact gtgctgctga agacatcgct 1260

gtcattgaga acatgaagta g 1281

<212> Type : DNA

<211> Length : 1281

SequenceName : 8

SequenceDescription :

Sequence

--------

<213> OrganismName : Cacna1g

<400> PreSequenceString :

atggacgagg aggaggatgg agcgggcgcc gaggagtcgg gacagccccg tagcttcacg 60

cagctcaacg acctgtccgg ggccgggggc cggcaggggc cggggtcgac ggaaaaggac 120

ccgggcagcg cggactccga ggcggagggg ctgccgtacc cggcgctagc cccggtggtt 180

ttcttctact tgagccagga cagccgcccg cggagctggt gtctccgcac ggtctgtaac 240

ccatggttcg agcgagtcag tatgctggtc attcttctca actgtgtgac tctgggtatg 300

ttcaggccgt gtgaggacat tgcctgtgac tcccagcgct gccggatcct gcaggccttc 360

gatgacttca tctttgcctt ctttgctgtg gaaatggtgg tgaagatggt ggccttgggc 420

atctttggga agaaatgtta cctgggagac acttggaacc ggcttgactt tttcattgtc 480

attgcaggga tgctggagta ttcgctggac ctgcagaacg tcagcttctc cgcagtcagg 540

acagtccgtg tgctgcgacc gctcagggcc attaaccggg tgcccagcat gcgcattctc 600

gtcacattac tgctggacac cttgcctatg ctgggcaacg tcctgctgct ctgtttcttc 660

gtctttttca tctttggcat cgtgggcgtc cagctgtggg caggactgct tcgcaaccga 720

tgcttcctcc ccgagaactt cagcctcccc ctgagcgtgg acctggagcc ttattaccag 780

acagagaatg aggacgagag ccccttcatc tgctctcagc ctcgggagaa tggcatgaga 840

tcctgcagga gtgtgcccac actgcgtggg gaaggcggtg gtggcccacc ctgcagtctg 900

gactatgaga cctataacag ttccagcaac accacctgtg tcaactggaa ccagtactat 960

accaactgct ctgcgggcga gcacaacccc ttcaaaggcg ccatcaactt tgacaacatt 1020

ggctatgcct ggatcgccat cttccaggtc atcacactgg agggctgggt cgacatcatg 1080

tacttcgtaa tggacgctca ctccttctac aacttcatct acttcattct tctcatcatc 1140

gtgggctcct tcttcatgat caacctgtgc ctggtggtga ttgccacgca gttctccgag 1200

accaaacagc gggagagtca gctgatgcgg gagcagcgtg tacgattcct gtccaatgct 1260

agcaccctgg caagcttctc tgagccaggc agctgctatg aggagctact caagtacctg 1320

gtgtacatcc tccgaaaagc agcccgaagg ctggcccagg tctctagggc tataggcgtg 1380

cgggctgggc tgctcagcag cccagtggcc cgtagtgggc aggagcccca gcccagtggc 1440

agctgcactc gctcacaccg tcgtctgtct gtccaccacc tggtccacca ccatcaccac 1500

caccatcacc actaccacct gggtaatggg acgctcagag ttccccgggc cagcccagag 1560

atccaggaca gggatgccaa tgggtctcgc cggctcatgc taccaccacc ctctacaccc 1620

actccctctg ggggccctcc gaggggtgcg gagtctgtac acagcttcta ccatgctgac 1680

tgccacttgg agccagtccg ttgccaggca ccccctccca gatgcccatc ggaggcatct 1740

ggtaggactg tgggtagtgg gaaggtgtac cccactgtgc ataccagccc tccaccagag 1800

atactgaagg ataaagcact agtggaggtg gcccccagcc ctgggccccc caccctcacc 1860

agcttcaaca tcccacctgg gcccttcagc tccatgcaca agctcctgga gacacagagt 1920

acgggagcct gccatagctc ctgcaaaatc tccagccctt gctccaaggc agacagtgga 1980

gcctgcgggc cggacagttg tccctactgt gcccggacag gagcaggaga gccagagtcc 2040

gctgaccatg tcatgcctga ctcagacagc gaggctgtgt atgagttcac acaggacgct 2100

cagcacagtg acctccggga tccccacagc cggcggcgac agcggagcct gggcccagat 2160

gcagagccta gttctgtgct ggctttctgg aggctgatct gtgacacatt ccggaagatc 2220

gtagatagca aatactttgg ccggggaatc atgatcgcca tcctggtcaa tacactcagc 2280

atgggcatcg agtaccacga gcagcccgag gagctcacca acgccctgga aatcagcaac 2340

atcgtcttca ccagcctctt cgccttggag atgctgctga aactgcttgt ctacggtccc 2400

tttggctaca ttaagaatcc ctacaacatc tttgatggtg tcattgtggt catcagtgtg 2460

tgggagattg tgggccagca gggaggtggc ctgtcggtgc tgcggacctt ccgcctgatg 2520

cgggtgctga agctggtgcg cttcctgccg gccctgcagc gccagctcgt ggtgctcatg 2580

aagaccatgg acaacgtggc caccttctgc atgctcctca tgctgttcat cttcatcttc 2640

agcatcctgg gcatgcatct ctttggttgc aagttcgcat ctgaacggga tggggacacg 2700

ttgccagacc ggaagaattt cgactccctg ctctgggcca tcgtcactgt ctttcagatt 2760

ctgactcagg aagactggaa taaagtcctc tacaacggca tggcctccac atcgtcttgg 2820

gctgctcttt acttcatcgc cctcatgact tttggcaact atgtgctctt taacctgctg 2880

gtggccattc ttgtggaagg cttccaggca gaggaaatcg gcaaacggga agatgcgagt 2940

ggacagttaa gctgtattca gctgcctgtc aactctcagg ggggagatgc caccaagtct 3000

gagtcagagc ctgatttctt ttcgcccagt gtggatggtg atggggacag aaagaagcgc 3060

ttggccctgg tggctttggg agaacacgcg gaactacgaa agagcctttt gccacccctc 3120

atcatccata cggctgcgac accaatgtca ctacccaaga gctccagcac aggtgtgggg 3180

gaagcactgg gctctggctc tcgacgtacc agtagcagtg ggtccgctga gcctggagct 3240

gcccaccatg agatgaaatc tccgccaagt gcccgcagct ccccgcacag tccctggagt 3300

gcggcaagca gctggaccag caggcgctcc agcaggaaca gcctgggccg ggcccccagc 3360

ctaaagcgga ggagcccgag cggggagcgg aggtccctgc tgtctggaga gggccaggag 3420

agtcaggatg aggaggaaag ttcagaagag gaccgggcca gcccagcagg cagtgaccat 3480

cgccacaggg gttccttgga acgtgaggcc aagagttcct ttgacctgcc tgacactctg 3540

caggtgccgg ggctgcaccg cacagccagc ggccggagct ctgcctctga gcaccaagac 3600

tgtaatggca agtcggcttc agggcgtttg gcccgcaccc tgaggactga tgacccccaa 3660

ctggatgggg atgatgacaa tgatgaggga aatctgagca aaggggaacg catacaagcc 3720

tgggtcagat cccggcttcc tgcctgttgc cgagagcgag attcctggtc ggcctatatc 3780

tttcctcctc agtcaaggtt tcgtctcctg tgtcaccgga tcatcaccca caagatgttt 3840

gaccatgtgg tcctcgtcat catcttcctc aactgtatca ccatcgctat ggagcgcccc 3900

aaaattgacc cccacagcgc tgagcgcatc ttcctgaccc tctccaacta catcttcacg 3960

gcagtctttc tagctgaaat gacagtgaag gtggtggcac tgggctggtg ctttggggag 4020

caggcctacc tgcgcagcag ctggaatgtg ctggacggct tgctggtgct catctccgtc 4080

atcgacatcc tggtctccat ggtctccgac agcggcacca agatccttgg catgctgagg 4140

gtgctgcggc tgctgcggac cctgcgtcca ctcagggtca tcagccgggc ccagggactg 4200

aagctggtgg tagagactct gatgtcatcc ctcaaaccca ttggcaacat tgtggtcatt 4260

tgctgtgcct tcttcatcat ttttggaatt ctcggggtgc agctcttcaa agggaagttc 4320

ttcgtgtgtc agggtgagga caccaggaac atcactaaca aatccgactg cgctgaggcc 4380

agctaccgat gggtccggca caagtacaac tttgacaacc tgggccaggc tctgatgtcc 4440

ctgtttgtgc tggcctccaa ggatggttgg gttgacatca tgtatgatgg gctggatgct 4500

gtgggtgtgg atcagcagcc catcatgaac cacaacccct ggatgctgct atacttcatc 4560

tccttcctcc tcatcgtggc cttctttgtc ctgaacatgt ttgtgggcgt ggtggtggag 4620

aacttccata agtgcagaca gcaccaggag gaggaggagg cgaggcggcg tgaggagaag 4680

cgactacgga ggctggagaa aaagagaagg aatctaatgt tggacgatgt aattgcttcc 4740

ggcagctcag ccagcgctgc gtcagaagcc cagtgcaagc cctactactc tgactactcg 4800

agattccggc tccttgtcca ccacctgtgt accagccact acctggacct cttcatcact 4860

ggtgtcatcg ggctgaacgt ggtcactatg gccatggaac attaccagca gccccagatc 4920

ctggacgagg ctctgaagat ctgcaattac atctttaccg tcatctttnc ttttgagaaa 4980

tatagcaaaa taccctaccg gggggagggg ggcagatttt ccaggtggaa ccagctggac 5040

ctggctattg tgcttctgtc catcatgggc atcacactgg aggagattga ggtcaatgct 5100

tcgctgccca tcaaccccac catcatccgt atcatgaggg tgctccgcat tgctcgagtt 5160

ctgaagctgt tgaagatggc tgtgggcatg cgggcactgc tggacacggt gatgcaggcc 5220

ctgccccagg tggggaacct gggacttctc ttcatgttat tgtttttcat ctttgcagct 5280

ctgggcgtgg agctctttgg agacctggag tgtgatgaga cacacccttg tgagggcttg 5340

ggtcggcatg ccacctttag gaactttggt atggcctttc tgaccctctt ccgagtctcc 5400

actggtgaca actggaatgg tattatgaag gacaccctcc gggactgtga ccaggagtcc 5460

acctgctaca acactgtcat ctcccctatc tactttgtgt ccttcgtgct gacggcccag 5520

tttgtgctgg tcaacgtggt catagctgtg ctgatgaagc acctggaaga aagcaacaaa 5580

gaggccaagg aggaggccga gctcgaggcc gagctggagc tggagatgaa gacgctcagc 5640

ccgcagcccc actccccgct gggcagcccc ttcctctggc ccggggtgga gggtgtcaac 5700

agtcctgaca gccctaagcc tggggctcca cacaccactg cccacattgg agcagcctcg 5760

ggcttctccc ttgagcaccc cacgatggta ccccaccccg aggaggtgcc agtcccccta 5820

ggaccagacc tgctgactgt gaggaagtct ggtgtcagcc ggacgcactc tctgcccaat 5880

gacagctaca tgtgccgcaa tgggagcact gctgagagat ccctaggaca caggggctgg 5940

gggctcccca aagcccagtc aggctccatc ttgtccgttc actcccaacc agcagacacc 6000

agctgcatcc tacagcttcc caaagatgtg cactatctgc tccagcctca tggggccccc 6060

acctggggcg ccatccctaa actaccccca cctggccgct cccctctggc tcagaggcct 6120

ctcaggcgcc aggcagcaat aaggactgac tccctggatg tgcagggcct gggtagccgg 6180

gaagacctgt tgtcagaggt gagtgggccc tcctgccctc tgacccggtc ctcatccttc 6240

tggggcgggt cgagcatcca ggtgcagcag cgttccggca tccagagcaa agtctccaag 6300

cacatccgcc tgccagcccc ttgcccaggc ctggaaccca gctgggccaa ggaccctcca 6360

gagaccagaa gcagcttaga gctggacacg gagctgagct ggatttcagg agacctcctt 6420

cccagcagcc aggaagaacc cctgtcccca cgggacctga agaagtgcta cagtgtagag 6480

acccagagct gcaggcgcag gcctgggtcc tggctagatg aacagcggag acactccatt 6540

gctgtcagct gtctggacag cggctcccaa ccccgcctat gtccaagccc ctcaagcctc 6600

gggggccaac ctcttggggg tcctgggagc cggcctaaga aaaaactcag cccacccagt 6660

atctctatag accccccgga gagccagggc tctcggcccc catgcagtcc tggtgtctgc 6720

ctcaggagga gggcgccggc cagtgactct aaggatccct cggtctccag cccccttgac 6780

agcacggctg cctcaccctc cccaaagaaa gacacgctga gtctctctgg tttgtcttct 6840

gacccaacag acatggaccc ctga 6864

<212> Type : DNA

<211> Length : 6864

SequenceName : 9

SequenceDescription :

Sequence

--------

<213> OrganismName : Ptgs2

<400> PreSequenceString :

atgacgatcc gacaccaagg ccagcagtac aggccgagga tggcatttct tcaaaagatt 60

gaagcactcg tgaaggacat gcagaaccca gagacgggag tcagaatgca gaaccagaga 120

gtcttggtca ccagtgtccc tcatgccatg acaggaggcg atgttctgca atggatcatc 180

cagcgccttt ggatctctaa cctcgaggcg cagaacttgg gtaacttcat cgtcaagtac 240

ggctacatct accctttgca agacccgaag aatctcgtcc tcaaacctga cagcagcctc 300

tatcgatttc agacgccgta cttctggccc actcagcagt ggccagctga ggacaccgac 360

tatgccatct atctagctaa gcgaaatatc aagaagaagg ggattttaga agaatatgaa 420

aaggaaaact atgatttctt gaacaagaag attaattaca agtgggattt tgtcatcatg 480

caggccaagg agcagtaccg gactggaaag gagaggaaca aagctgacag gtatgcgctg 540

gattgccagg agaaggcgta ttggctggtg caccgaagcc ctcctggaat gaacaatgtg 600

ttagactacg gcctggaccg cgtgaccaat ccaaacgaag ttaagaaaca aacagtcact 660

gctgtcagaa aggagattat gtattaccag caggccttaa tgaggtccac agtgaagtct 720

tcggtgtccc ttggaggaat cgtcaagtat agtgagcagt tctcgtccaa tgatgccatc 780

atgtctggct gccttccaag taatccttgg atcacagacg acacccagtt ctgggattta 840

aacgccaagt tggtggaggt cccaaccaag atgcgggtgg agagatgggc tttcaacttc 900

agcgaactga ttcgagaccc caagggccgg cagagcttcc agtactttct caagaaggaa 960

ttcagtgggg agaacctggg attctgggaa gcctgtgagg atctgaagta tggggatcag 1020

tccaaggtca aggagaaggc agaggagatc tacaagctgt tcctggcacc gggtgcaagg 1080

cgatggatca acatagacgg caaaaccatg gacatcaccg tgaaggggct gagacacccc 1140

caccgctatg tgttggacgc ggcgcagacc cacatttaca tgctcatgaa gaaggattct 1200

tatgctcgct atttgaaatc tccgatctat aaagaaatgc tggccaaagc tatcgagcca 1260

caggaaacca ccaagagaag ctccaccctc ccatttatga gacgccacct acgctccagc 1320

ccaagccctg tcattctgcg gcagctggaa gaagaagaga gagctcgaga agcagccaac 1380

accgtggaca tcacccagcc aggtcagcac ttggctccca gccctcacct ggccgtgtac 1440

acagggacct gcgtgcctcc ctctccctcc agccccttct caccatcctg ccgctcgccc 1500

aggaagccct tcccttcccc aagtcgcttc atccggcggc ccagcattgc catctgcccc 1560

tcgcccatcc gagtggccct ggagggttct tcagggctgg agggcaaggg ggaagccagc 1620

tggtctggtg ccaaccctgg gcccccagtc actgagagca tcgagacctc tgtagaccgc 1680

tcacgaccac acagccagcc cagggccccg ctcaaggccc gcgctgccct gtcccttggc 1740

aggtttctga gacggggctg tttggcatca ccagtttttg ccaggctctc accgaagtgc 1800

ccttctgtgt cacacgggaa ggtgcagcct ctgggggaca tgggtcaaca gctgcccagg 1860

ctgaaaccca agaaagtagc caactttttc cagatcaaaa tggaaatgcc tacggacagt 1920

gggccctgcc tgatggactc ggatgacccc ggagcaggag agtctggcga ccagaccacg 1980

gaaaaggaag tcatctgccc ctgggaaagc ctggcggaag ggaaggcggg ctga 2034

<212> Type : DNA

<211> Length : 2034

SequenceName : 10

SequenceDescription :

Sequence

--------

<213> OrganismName : Kcnk2

<400> PreSequenceString :

ggtttatatc cggaatccca tggaattgtc ggtaattcaa tgtatgatcc tgtctttgat 60

gcttcgttcc atctacgagg gcgagagaag tttaatcata ggtggtgggg aggccaaccg 120

ctatggatta cagccaccaa gcaaggggtg agagctggaa cattcttttg gtctgtgagc 180

atccctcatg aacggaggat cctaaccatt cttcagtggc tttctctgcc agacaacgag 240

aggccttcag tttatgcctt ctactcagag cagcctgatt tttctggaca caagtacggc 300

ccttttggcc ctgagatgac aaatcctctg agggagattg acaagaccgt ggggcagtta 360

atggatggac tgaaacaact caggctgcat cgctgtgtga acgttatctt tgttggagac 420

catggaatgg aagatgtgac atgtgacaga actgagttct tgagcaacta tctgactaat 480

gtggatgaca ttactttagt gcctggaact ctgggaagaa ttcgagccaa atctatcaat 540

aattctaaat atgaccctaa aaccattatt gctaacctca cggtaagcct ctgcccttgg 600

agcagcttcg ataggctcac cttcccatgt ttgccttccc ggtgggtggt taggaacatt 660

ttcctcactg acctgcaaat cctaactgat catacaattg ttttaaggaa acctttggac 720

gtttataaga aaccatcagg aaaatgtttt ttccagggtg accacggctt tgataacaag 780

gtcaatagca tgcagactgt tttcgtaggt tatggcccaa cttttaagta caggactaaa 840

gtgcctccat ttgaaaacat tgaactttac aatgttatgt gcgatctcct aggcttgaag 900

cccgctccca ataatggaac tcatggaagc ttgaatcacc tactgcgtac aaataccttt 960

aggccaacca tgccagacga agtcagccga cctaactacc cagggattat gtaccttcag 1020

tccgagtttg acctgggctg cacctgtgac gataaggtag agccaaagaa caaattggaa 1080

gaactcaata aacgtcttca taccaaagga tcaacagaag ctgaaaccgg gaaattcaga 1140

ggcagcaaac atgaaaacaa gaaaaacctt aatggaagtg ttgaacctag aaaagagaga 1200

catctcctgt atggacggcc tgcagtgctc tatcggacta gctatgatat cttataccat 1260

acggactttg aaagtggtta tagtgaaata ttcttaatgc ctctctggac atcgtatacc 1320

atttctaagc agggatgcag agcgactgag tgcaagtgtt tggtgaagct gctaagggga 1380

gataaaagaa tagatccact ggcttcaaac tctgattttc tgttgagatt ggtaagcaat 1440

ggactctgtt tgttctccca cctgatctcc tccccagaag ctaagtatga tgcattcctc 1500

gtaaccaaca tggttccaat gtaccccgcc ttcaaacgtg tttgggctta tttccaaagg 1560

gttttggtga agaaatatgc ttcagaaagg aatggagtca acgtaataag tggaccgatt 1620

tttgactaca attacgatgg cctacgtgac actgaagatg aaattaaaca gtatgtggaa 1680

ggcagctcta tacctgtccc cacccactac tacagcatca tcaccagctg cctggacttc 1740

actcagcctg cagacaagtg tgacggtccc ctctctgtgt cttccttcat ccttcctcac 1800

cgacccgaca atgatgagag ctgtaatagc tccgaggatg agtcgaagtg ggtagaggaa 1860

ctcatgaaga tgcacacagc tcgggtgcgg gacattgagc acctcactgg tctggatttc 1920

taccggaaga ctagccgtag ctattcggaa attctgaccc tcaagacata cctgcataca 1980

tatgagagcg agatttaa 1998

<212> Type : DNA

<211> Length : 1998

SequenceName : 11

SequenceDescription :

Sequence

--------

<213> OrganismName : Rag1

<400> PreSequenceString :

atggctgtcc ccttgccatc taccctgaga ctcagttctg cacctgatga aattcagcac 60

ccgcacatca aattttccga gtggaaattt aagctgttta gggtgagatc ctttgaaaag 120

gcacccgaag aagcacaaaa agagaaggat tcctcagaag ggaaaccttg tctcgagcag 180

tctccagtag ttctagataa gcctgggggt cagaattcag ttctgactca acgagcattg 240

aaactccatc ctaaattttc aaagaaattc catgttgatg ggaagtcaag cgacaaagca 300

attcaccaag ccaggcttag acacttctgc cgcatctgtg gcaatcactt caagagtgac 360

gggcacaacc ggagataccc agtccacggg cccgtggacg ctaaaactca aagccttttc 420

cgaaagaagg aaaaaagagt cacgtcctgg ccagatctca ttgccagagt tttccggatt 480

gatgtgaagt cagatgttga ctccatccac cccactgaat tctgccataa ctgttggagc 540

attatgcaca ggaagttcgg cagtgctcac agtcaggtct actgcccaag gaatgtgacc 600

gtggagtggc acccccacac accgtcctgt gacatctgct ttactgccca tcggggactg 660

aagaggaaga gacatcagcc caacgtgcag ctcagcaaga aactaaaaac tgtgctcaac 720

catgctagac gggaccgtcg caagagaact caggctaggg tcagcagcaa ggaggtcatg 780

aagaagatct ccaactgcag taagattcat ctcagtacca agcttcttgc agtagacttc 840

ccagcacact ttgtgaaatc catctcctgc cagatatgtg aacacattct ggccgatccg 900

gtggagacca cctgtaagca cctgttctgt agaatatgca ttctccggtg cctcaaggtc 960

atgggcagct attgtccctc gtgccgatat ccatgcttcc ctactgacct gcagagtccg 1020

gtcaagtcct ttctgagcat cttgaattct ctgatggtga agtgtcctgc ccaagagtgc 1080

aatgaggaag taagtctgga aaaatataac caccatgtct caagccacaa agaatctaaa 1140

gagactttgg tgcatattaa taaagggggc cggcctcgcc agcatctcct gtcactgacg 1200

cgaagggctc agaaacatcg gctgagggag ctcaagattc aagtcaaaga atttgcagac 1260

aaagaagaag gtggagatgt gaagtctgtg tgcttgacgt tgttcctgct ggcactgagg 1320

gcaaggaatg agcacagaca agctgatgag ctcgaggcca tcatgcaagg ccggggctcc 1380

ggtcttcaac cagctgtttg cttggccatc cgtgtcaata ccttcctcag ctgtagccaa 1440

taccataaga tgtacaggac ggtgaaagct atcactggga ggcagatttt tcaacctttg 1500

cacgctcttc gcaatgctga gaaggtcctc ctaccaggct accacccctt tgagtggcag 1560

ccccctctga agaacgtgtc ctccagaact gacgttggaa ttattgatgg gctctctgga 1620

cttgcctcct ccgtagatga atacccagtg gacacaattg caaagaggtt ccgctatgac 1680

tctgctttgg tgtctgcttt gatggacatg gaggaagaca tcttggacgg catgagatcc 1740

caagatcttg atgactacgt gagtggtccc ttcaccgtgg tggtaaagga atcgtgtgat 1800

ggaatggggg atgtgagtga gaagcacggg agtgggcccg cagttccaga aaaggcagtt 1860

cggttctctt tcaccgtcat gagagttaca gtagagcatg gctctcagaa cgtgaagata 1920

tttgaggaac ccaagcccaa ttctgaactg tgttgcaagc cactgtgcct tatgctggca 1980

gatgagtctg accacgagac acttactgct attctcagcc ccctcatcgc tgagagggag 2040

gccatgaaga gcagtgaatt gctgctggag atgggaggca tccccaggac ttttaaattt 2100

atcttcaggg gcactggata tgacgagaaa cttgtcaggg aagtagaagg cttggaagct 2160

tctggctcag tctacatttg tacactttgt gatgccaccc gtctggaagc ctcccaaaat 2220

cttgtcttcc actccataac cagaagccac gccgagaatc tgcagcgcta tgaggtctgg 2280

cggtccaatc cctatcacga gtcagtggaa gaactccggg accgggtgaa aggagtctct 2340

gccaaacctt tcatcgagac agtcccttcc atagatgcgc ttcactgtga cattggaaat 2400

gcagctgaat tctataagat tttccagctg gagatagggg aagtgtataa aaatcccaat 2460

gcctctaaag aggaaaggaa gagatggcag gccaccttgg acaaacatct gcggaaaagg 2520

atgaacttaa aaccaatcat gaggatgaat ggcaactttg cccggaagct tatgacccaa 2580

gagactgtgg acgcagtttg tgagttaatt ccttctgagg agaggcacga agctctcagg 2640

gagctgatgg acctttacct gaagatgaaa ccggtgtggc gctcatcgtg tcctgctaaa 2700

gagtgcccgg agtccctttg tcagtacagt ttcaactcac agcgtttcgc tgagctcctc 2760

tccaccaagt tcaaatacag atacgagggc aaaatcacca attactttca caaaaccttg 2820

gcacacgtcc ctgaaattat tgaaagggat ggctctattg gggcatgggc aagtgagggc 2880

aatgagtctg gtaacaaact gtttaggcgc ttccggaaaa tgaatgccag gcagtccaag 2940

tgctatgaga tggaagatgt cctgaaacac cactggctgt atacttcaaa atacctccag 3000

aagtttatga atgcccataa tgcattaaaa aactctgggt ttaccatgaa ctcaaaggag 3060

actttagggg actctttgga cattgaggac tctctggaaa gccaagattc aatggagttt 3120

taa 3123

<212> Type : DNA

<211> Length : 3123

SequenceName : 12

SequenceDescription :

Sequence

--------

<213> OrganismName : Sstr4

<400> PreSequenceString :

atgaacacgc ctgcaactct gcccctgggg ggcgaggaca ccacctggac ccctgggatc 60

aacgccagct gggctccgga tgaggaggag gatgcagtgc ggtccgacgg cacggggaca 120

gcgggcatgg taactatcca gtgcatctat gcgctcgtgt gtctggtggg cctggtagga 180

aacgccctgg tcatattcgt gatcctacgc tatgccaaaa tgaagacagc caccaacatc 240

tacctgctca acctggccgt cgctgatgag ctcttcatgc tcagtgtgcc atttgtggcc 300

tcggcggctg ccctgcgcca ctggccgttc ggggcggtgc tgtgccgcgc agtgcttagt 360

gtggacggcc ttaacatgtt cacgagtgtc ttctgcctca cagtgctcag cgtggatcgc 420

tatgtggctg tagtgcaccc tctgcgagct gccacctacc ggcggcccag cgtggccaag 480

ctaatcaacc tgggagtgtg gctagcatcc ttgctggtca ccctgcccat cgcagtcttc 540

gctgacacta ggccagctcg tgggggtgag gcagtagctt gcaacctgca ctggcctcac 600

ccggcctggt ctgcagtctt tgtgatctat acttttttgc tgggcttcct actcccggtt 660

ctggctatcg gattatgtta cctgcttatc gtgggcaaga tgcgtgctgt ggccctgcgg 720

gctggctggc aacaacggag gcgctcagag aagaagatca ctaggctcgt gctaatggtg 780

gtgactgtct ttgtgctatg ctggatgcca ttctatgtag tgcagcttct gaatctgttt 840

gtcaccagcc tcgatgccac tgtcaaccat gtgtccctca tcctcagcta tgccaacagc 900

tgtgccaacc cgattctcta tggtttcctc tcagacaact tccgacgctc tttccagcgg 960

gttctgtgcc tgcgctgctg tctcctggaa acaactggag gtgctgagga agagcccctg 1020

gactactatg ctactgctct caaaagcaga ggtggcccag gatgcatatg ccctccattg 1080

ccctgccagc aggagcccat gcaagcagaa cctgcctgca agcgagtccc tttcaccaag 1140

accactactt tctga 1155

<212> Type : DNA

<211> Length : 1155

SequenceName : 13

SequenceDescription :

Sequence

--------

<213> OrganismName : Ptgs2

<400> PreSequenceString :

gaccccaagt tacgtgaact tctggatgtt gggaacatcg gacacttgga acagcgcatg 60

ataactgtgg tgtatgggcc agacctggtg aatatctccc acctgaatct tgtggctttt 120

caagaggaag tggccaagga atggacaaat gaggttttca gtttggcaac aaacctgctg 180

gctcagaaca tgtccaggga cgcatttctg gagaaagcat atactaagct caagcttcag 240

gtgaccccag aagggcgcat tcctcttaaa aacatctatc gactgttttc ggcagaccgg 300

aagcgagtgg aaactgcgct agaggcctgt agtcttccat cgtcaaggaa cgattccatc 360

cctcaagagg actttactcc agatgtatac agagttttcc tgaacaatct ttgtccccga 420

cctgaaattg ataacatctt ctctgaattt ggtgccaaaa gcaaaccgta ccttactgtt 480

gatcagatga tggattttat caaccttaag cagagagatc cccggctgaa tgaaatactt 540

tacccacctc tgaagcaaga gcaggtccaa gtgttgattg agaagtacga gcccaacagc 600

agcctcgcca agaaagggca gatgtcagtg gatggattca tgcgctacct gagcggagaa 660

gaaaatggag tcgtttcacc tgagaaactg gatttgaacg aagacatgtc tcagcccctg 720

tctcactatt tcatcaattc ctcacacaac acctacctca cagctggcca gttggctggg 780

aactcgtctg tagagatgta tcgccaggtg cttctgtctg gatgtcgctg tgtggagctg 840

gactgctgga agggcaggac cgctgaggaa gagcctgtca tcacccatgg attcaccatg 900

acaacagaaa tatccttcaa ggaagtcata gaagccatcg cagagtgtgc gttcaagact 960

tctccttttc ccatcctcct ttcctttgag aaccatgtgg attccccgaa gcaacaagcc 1020

aagatggccg agtattgccg attaatcttt ggtgatgccc tccttatgga gccactggaa 1080

aaatacccac tggaatctgg ggtacctctt ccaagcccta tggatttaat gtataaaatc 1140

ttggtgaaaa acaagaagaa gtcacacaag tcgtcagagg gaagtggtaa aaagaagctc 1200

tctgagcaag cttccaacac gtacagcgac tcttccagcg tgttcgagcc ttcgtctccg 1260

ggagctgggg aagcagatac ggagagtgat gacgatgacg acgatgatga ctgtaaaaag 1320

tcttccatgg atgaggggac tgctggcagc gaggccatgg ccacagaaga gatgtctaac 1380

ctggtgaact atattcagcc tgtcaagttt gagtcctttg aaacttcaaa aaaaagaaat 1440

aaaagctttg aaatgtcttc cttcgtggaa accaaaggac tcgaacaact cacgaagtct 1500

ccagttgaat ttgtcgaata caacaagatg cagcttagca ggatatatcc caaaggaaca 1560

cgcgtggact catccaacta catgcctcag ctcttctgga atgctggctg tcagatggtg 1620

gcgctcaact tccagacagt ggatctagct atgcagataa acatgggcat gtacgaatac 1680

aatgggaaga gtggctacag gctgaagcca gagttcatga ggaggccaga caagcatttt 1740

gatccattta ctgaaggaat cgtagatggg atagtggcca acactttatc tgttaagatt 1800

atttcaggtc agtttctctc tgataagaaa gttgggactt atgtggaagt ggatatgttt 1860

ggtttgcctg tggacacaag aaggaaggca tttaaaacca agacatccca aggaaatgct 1920

gtaaatcctg tctgggaaga agagccaatt gtattcaaaa aggtagttct gccttctctg 1980

gcctgtttaa ggatagcagc atatgaggaa ggaggcaaat ttattggcca ccggatcttg 2040

cctgtgcaag caattcggcc aggctatcac tacatctgcc tgcggaatga gaggaaccag 2100

cccctgatgc tgccagctgt ctttgtctac atagaagtca aagactatgt cccagacacg 2160

tatgcagatg taattgaagc attatcaaac ccaatccgat atgtcaatct gatggaacag 2220

agagctaagc agttggctgc attgacactg gaggatgaag aggaagtcaa gaaggaggct 2280

gaccccgggg aaacgtcatc cgaggctcca agtgaaacca ggacaactcc agcagagaat 2340

ggggtgaatc acaccgcaac ccttgcaccc aagccacctt cccaggctcc acacagccag 2400

cctgctccag ggtctgtgaa ggcacccgcc aaaacagagg atctgattca gagcgtgtta 2460

acagaagtag aggcgcagac cattgaagag ctcaagcaac agaaatcgtt cgtgaaactt 2520

caaaagaagc actacaaaga aatgaaagac ctggtcaaga gacaccacaa gaaaaccacc 2580

gagctcatca aggagcatac caccaagtac aatgagattc agaatgacta cttgagaagg 2640

agggcagcct tggaaaagtc cgccaaaaag gatagcaaga agaaatctga acccagcagc 2700

ccagatcatg gctcatccgc cattgagcaa gacctcgcgg ccctggatgc agaaatgact 2760

cagaagttga tagacttgaa agacaagcaa caacagcagc tgcttaatct tcggcaagag 2820

cagtattaca gtgagaagta ccagaagcgg gagcacatta aattggtaag ccagaagctg 2880

ccgggctctg cagaggag 2898

<212> Type : DNA

<211> Length : 2898

SequenceName : 14

SequenceDescription :

Sequence

--------

<213> OrganismName : Mapt

<400> PreSequenceString :

atggctgaac cccgccagga gtttgacaca atggaagacc aggccggaga ttacactatg 60

ctccaagacc aagaaggaga catggaccat ggcttaaaag agtctccccc acagccccca 120

gccgatgatg gatcagaaga accagggtcg gagacctctg atgctaagag cactccaact 180

gctgaagacg tgactgcgcc cctagtggaa gagagagctc ccgacaagca ggcgactgcc 240

cagtcccaca cggagatccc agaaggcacc acagctgaag aagcaggcat cggagacacc 300

ccgaacatgg aggaccaagc tgctgggcat gtgactcaag aaccgcagaa ggtcgagatt 360

ttctcacaaa gtttgcttgt cgaaccaggg cgtcgtgaag gccaggcccc agattcaggg 420

atctcagact ggacccacca acaggtgcct agcatgtctg gggctcccct cccgccacaa 480

ggcctcagag aggctacaca ccaacctttg ggaacaagac ctgaggatgt agagagaagt 540

cacccggcct ctgagctgct gtggcaagag tcacctcaga aggaagcctg gggcaaagac 600

aggctgggaa gtgaggagga ggtggacgaa gacatcacca tggatgaatc gtcccaggaa 660

tcccctccct cccaagcctc cctggcccct ggcacggcca cccctcaggc ccgctccgtt 720

tctgcttccg gggtctctgg ggagaccact agcatccctg gattcccagc tgagggttcc 780

atcccacttc ctgctgattt cttctccaaa gtctccgcag agacccaggc ctcaccgcca 840

gaagggcctg gcacggggcc ctcggaggaa gggcacgagg ctgcccctga gttcacattc 900

cacgtggaaa tcaaagccag cgcgccgaag gaacaggatt tggaaggggc tacagtggtg 960

ggggcccctg cagaggagca aaaggcccga ggcccctctg tgggaaaggg cacgaaagag 1020

gctagccttc tggaaccaac tgacaagcaa cctgcagccg gcctgccagg aaggccagtc 1080

agtcgggttc ctcaacttaa agctcgagtg gccggcgtaa gcaaagacag gacaggaaat 1140

gacgagaaga aagccaagac atccacacct tcctgtgcta aaaccccgag caataggcct 1200

tgccttagcc ccacacgtcc cactcctggt agctcagacc ctttgatcaa accctccagc 1260

cctgccgtgt gccctgagcc agccacctct cctaaatacg tctcttctgt cacaccccga 1320

aatggcagtc ctggaacaaa gcagatgaaa ctcaagggcg ccgatggcaa aacgggggcg 1380

aagatcgcca cacctcgggg agcagccact ccgggccaga aaggcacatc caatgccacc 1440

aggatcccag ccaagaccac acccagccca aagactcctc caggatcagg tgaaccacca 1500

aaatccggag aacgaagcgg ctacagcagc cccggctcgc ccggaacccc tggcagtcgc 1560

tcccgtaccc catccctacc aacgccgccc acccgagagc ccaaaaaggt ggcagtggtt 1620

cgcactcccc ctaagtcacc gtctgccagt aagagccgcc tacagactgc ccctgtgccc 1680

atgccagacc taaagaacgt caggtccaag attggctcca ctgagaacct gaagcaccag 1740

ccgggaggcg gcaaggtgca gataattaat aagaagctgg atcttagcaa cgtccagtcc 1800

aagtgtggct caaaggacaa tatcaaacac gtcccgggcg gaggcagtgt gcaaatagtc 1860

tacaagccag tggacctgag caaggtgacc tccaagtgtg gttccttagg gaacatccat 1920

cacaagccag gaggtggcca ggtagaagta aaatcagaga agctggactt caaggataga 1980

gtccagtcga agattggctc cttggataac atcacccatg tccctggagg agggaataag 2040

aagattgaaa cccacaagct gaccttcagg gagaatgcca aagccaagac agaccatgga 2100

gcagaaatcg tgtacaagtc acctgtggtg tctggggaca catctccacg gcacctcagc 2160

aacgtctcct ccacgggcag catcgacatg gtggactctc cacagcttgc cacgttagcc 2220

gatgaagtgt ccgcctcttt ggccaagcag ggaaaacctg tgttactgag ttctgaagtt 2280

tggaactaca gccatgattt tggccaccat acagacctgg gactttag 2328

<212> Type : DNA

<211> Length : 2328

SequenceName : 15

SequenceDescription :

Sequence

--------

<213> OrganismName : Ptprz1

<400> PreSequenceString :

atggattact tacaaaacaa tttccgagaa caacagtaca agttttccag gcaggtgttt 60

tcttcgtaca ctgggaagga agagattcat gaagcagtgt gcagttcaga accagaaaat 120

gtgcaagctg accctgagaa ttacaccagc cttctgatca catgggaaag gcctcgggtt 180

gtttatgaca caatgattga gaagtttgcg gttctgtacc agccactgga gggaaacgac 240

caaaccaagc atgagttttt aacagatggc tatcaggact tgggtgccat tctcaataac 300

ttaataccta acatgagtta tgtgctccaa atagtggcca tatgctctaa tggcctttat 360

ggaaagtaca gtgaccaatt gatagtcgac atgcctactg aggatgctga acttgacctc 420

tttcctgaat taattggaac tgaagaaata atcaaggagg aagactatgg aaaaggcaat 480

gaagaagaca ctggcttgaa tcccggtaga gacagtgcca caaaccaaat aaggaaaaag 540

gaaccccagg tttctaccac aactcactat aatcacatgg ggactaaata taatgaagcc 600

aagactaacc gatctccaac gaaaggatct gaattctctg gaaagagtga tgttctcaac 660

acatccctga atcctacttc ccaacaggtt gctgaattca atccagaaag agaaatgtcc 720

ttgccttctc agattggaac taacctgcca ccacacagtg tggaaggcac ctcagcctcc 780

ttaaacagtg gctctaaaac tctccttgtc ttcccacaga tgaacttgtc tgggactgca 840

gaatccttaa atatggtttc cataacagag tacaaagagg tgtctgctga cctcagtgag 900

gaagaaaact tactgactga tttcaagctc gatagtggag cagatgattc gtcaggctct 960

agccctgcgt cctccactgt ccccttttcc acagataatc tatcccatgg atatacatct 1020

tcttcagata cgcccgaggc ggtcacgtat gatgtcctta ggccagaatc tacgagaaat 1080

gctctagagg attcggctcc atcaggttca gaagaatcac taaaggatcc ctctcttgaa 1140

gggagtgtgt ggttccctgg atccacagac ctaacaacac agtctgagac tggatctggg 1200

agagagggct ttctccaagt taactccacg gacttccaag ttgatgaatc gagggagaca 1260

actgagacat tttctccaga tgctaccgcg tcccggggtc cttcggtcac agatatggaa 1320

atgccacatt attctacctt tgcctacccc ccgactgaag taacaccaca tgctttcact 1380

ccatcctcca gaccacttga tttggctccc actagcaaca tcctccattc gcagacaact 1440

caaccagtat acaatggtga gacacctctt caaccttcct acagtagtga agtctttcct 1500

ctagtcaccc ctttgttgct tgacaatcag accctcaaca ctacccctgc tgcttcaagt 1560

agtgattcgg ccttgcatgc tacgcctgta ttccccagtg ttggtgtgtc atttgactcc 1620

atcctgtctt cctatgatga tgcacctctg ctcccatttt cctctgcttc cttcagtagt 1680

gacttgtttc accatctgca tacggtttct caaacccttc cgcaagttac ttcagctgct 1740

gagagggatg agctgtcttt gcatgcttct ctgctggtgg ctgggggtga tttgctgtta 1800

gagcccagcc ttgttcagta ttctgatgtg atgtcacatc aggtcactat tcatgctgct 1860

tcggacacat tggaatttgg tagtgagtct gctgtccttt ataaaacgtc tatggtttct 1920

caaatcgaat cacccagcag tgatgtcgtt atgcatgcat attcgtcagg gcctgaaact 1980

tcttatgcca ttgagggctc ccaccacgtg ctcactgttt cttccagttc tgcaatacct 2040

gtgcatgatt ctattggtgt agctgatcag gggtccttac ttatcaatcc tagccatata 2100

tcactgcctg agtcctcatt tattactcca actgcatcat tactgcagct tcctcctgcc 2160

ctctctggtg atggggagtg gtctggagcc tcctctgata gtgaattgct tttacctgac 2220

acagatgggc tgagaactct taacatgtct tcacctgttt ctgtagctga ttttacatac 2280

acgacatctg tgtctggcga tgatattaag ccgctttcta aaggtgaaat gatgtatgga 2340

aatgagactg aactgaaaat gtcttctttc agtgacatgg catacccttc taaaagcaca 2400

gtcgtgccaa agatgtctga tattgtaaat aagtggagtg aatctttaaa agaaacctct 2460

gtttccgtat ctagcataaa cagcgtgttt acagagtctc tggtttatcc cataactaag 2520

gtttttgatc aggagattag tcgagttcca gagattatct tcccagttaa acctacacac 2580

acagcatctc aagcatctgg tgacacttgg cttaaacccg ggcttagcac aaactcagag 2640

cctgcgctct ctgacactgc ttctagtgaa gtattacacc cttcaacaca gcccttgctc 2700

tatgaggccg catctccttt taatacggaa gcattgctgc aaccttcctt tccggcttct 2760

gatgttgaca ccttgcttaa aactgccctt ccctctgggc ctcgtgatcc agtgctgact 2820

gaaaccccca tggttgagca aagtagctct tccatgtctc ttcccctggc atcagagtct 2880

gcttcaagca aaagcacgct gcactttaca tctgtaccag ttctcaatat gtcaccttct 2940

gatgtgcacc ccacttcact tcaacgctta acagttcctc actcgaggga ggaatatttt 3000

gaacaaggtt tgcttaagag caaaagtccc cagcaagtcc tgccgtcctt gcacagccat 3060

gacgagtttt tccaaactgc acatctggac attagccagg cctaccctcc aaaaggaagg 3120

catgcatttg ctactcctat tttatcaatc gatgaaccac aaaatacact tataagcagg 3180

cttgtgtatt ctgaggacat tttcatgcac cctgaaattt ctattactga taaggcactt 3240

actggtctac caacgaccgt ttctgatgta cttatagcta ctgaccattc tgttccatta 3300

ggaagtgggc ccatttccat gacaactgtt tctcccaaca gagatgattc tgtgaccaca 3360

accaagttgc ttcttccttc taaagctact tctaagccga ctcatagtgc cagatctgat 3420

gccgatttag taggaggtgg tgaagatggt gatgactatg atgatgatga ttatgatgac 3480

atagatagtg atcgctttcc cgtaaataag tgtatgtcat gttcacccta tagagaatca 3540

caggaaaagg taatgaatga ctcggacacc caagaaagca gtcttgtgga tcagagtgac 3600

ccaatttcac atttgctctc tgagaatacc gaagaagaaa atggaggcac gggtgtaact 3660

agggtggata aaagtcctga taagtcacca ccaccaagta tgctacccca gaagcacaat 3720

gatggaagag aggaccgtga cattcagatg ggtagtgctg tccttcctca caccccagga 3780

tctaaagcat gggcagtttt gacaagtgat gaagagagtg ggtcagggca aggcacctca 3840

gatagcctta atgataatga gacttccaca gatttcagtt tcccagatgt taatgaaaag 3900

gatgctgatg gtgtcctgga agcagatgac acaggcatag ctccgggatc tccacggtcc 3960

tccacaccat ctgttactag tgggcattca ggagtatcca acagttcaga ggcagaggcc 4020

agtaatagta gccatgagtc tcgtattggt ctagctgagg ggttggagtc tgagaagaag 4080

gcggttatac cccttgtgat cgtgtctgcc ctgactttta tctgtctagt ggttcttgtt 4140

ggtattctca tctactggag aaaatgcttc cagacggctc acttttattt agaagacaat 4200

acatcccctc gagtaatatc tacaccacca acacccatct ttccaatttc agatgatatt 4260

ggagcaattc caataaagca ttttccaaag cacgtagcag acttacatgc aagtaatggg 4320

ttcactgaag aatttgagac actgaaagag ttttaccagg aagtgcagag ctgcactgtt 4380

gacttaggta ttacagcaga cagctccaat cacccagaca acaagcacaa gaacagatat 4440

gtcaacatcg tagcctatga tcatagcagg gttaagctca cacaacttgc tgaaaaagat 4500

ggaaaactga ctgattacat caatgccaat tatgttgatg gttacaacag gccaaaggct 4560

tacattgctg cccagggtcc actgaagtcc acagcagaag atttctggag aatgatatgg 4620

gagcataatg tggaagtcat cgttatgata acaaacctcg tggagaaagg aagaagaaaa 4680

tgtgaccagt actggcctac tgatggcagt gaagaatacg ggagcttcct ggtcaaccag 4740

aagaatgttc aggtgcttgc ttattatact gtgaggaatt tcaccctaag aaacacaaag 4800

ataaaaaagg gctctcagaa aggaagatcc agtggacgcc tagtcactca gtatcattac 4860

acccagtggc ctgatatggg tgttcccgag tactccctac ccgtgctggc ctttgtgagg 4920

aagactgctc aggccaagcg acatgcagtc gggccagttg ttgtccactg cagcgctggc 4980

gtggggagaa cgggcacata cattgtacta gacagtatgc tacagcagat tcaacacgaa 5040

ggaaccgtca acatatttgg tttcttaaaa cacattcgtt ctcaaaggaa ttatttggtg 5100

caaacagagg agcaatacgt cttcattcac gacacactgg ttgaggccat actcagcaaa 5160

gagacagagg tgcctgacag ccacatccat tcctatgtta atacactcct catacccgga 5220

ccatcaggca agacaaaact agagaaacaa ttccagcttc tgagtcagtc aaatattctt 5280

cagagtgact actccacggc cctgaagcag tgtaacaggg agaagaacag aacatcctcc 5340

atcatccccg tggaaagatc gagggtgggc atttcatccc tgagtgggga aggcacagac 5400

tacatcaatg catcctatat tatgggttat taccagagca atgaattcat catcacccag 5460

cacccccttc tacacaccat caaggacttc tggagaatga tatgggatca taatgcccag 5520

ctggtggtta tgattccaga tggtcaaaac atggcagagg atgaatttgt ttactggcca 5580

aataaagatg agcccataaa ttgcgagagc ttcaaagtca cgcttatgtc tgaagaacac 5640

aaatgtcttt cgaatgagga aaaactgata gttcaagact tcatcttgga agcgacacag 5700

gatgattatg tgctggaagt aaggcatttt cagtgcccga aatggccaaa tccagatagt 5760

cccattagta aaacatttga acttataagt attatcaaag aagaagctgc taacagagat 5820

gggcccatga ttgttcacga tgagcatgga ggagtaacag cagggacatt ttgtgctctc 5880

acaaccctta tgcaccaact agaaaaagaa aactccatgg atgtttacca agtagccaag 5940

atgatcaatt tgatgaggcc gggagtcttc actgacattg agcagtacca gtttctctac 6000

aaagtggtcc tcagcctggt cagcacaagg caggaagaga atccatccac ctccctggat 6060

agtaatggtg cagctttgcc tgatggaaat atagctgaaa gcctggagtc tttagtctga 6120

<212> Type : DNA

<211> Length : 6120

SequenceName : 16

SequenceDescription :

Sequence

--------

<213> OrganismName : Map2k3

<400> PreSequenceString :

atggagtcgc ccgccgccag cccgccggcc agcttgcctc agaccaaagg aaaatccaaa 60

aggaaaaggg atttacggat atcctgtgtg tccaagccac ccgtgtccaa ccccacaccc 120

ccccggaacc tggactcccg gaccttcatc actatcggag acagaaactt cgaagtggag 180

gctgatgact tggtgaccat ctcagagctg ggccgcggag cctatggggt ggtggagaag 240

gtgcggcatg ctcagagtgg taccatcatg gctgtcaagc gcatccgggc cactgtgaac 300

tcacaggagc agaaacgtct gcttatggac ctagacatca acatgcgcac tgtcgactgc 360

ttctatactg tcaccttcta tggtgccctc tttagagagg gggatgtgtg gatctgcatg 420

gaactcatgg acacgtccct ggataagttc taccggaagg tgctagagaa gaacatgaaa 480

attccggaag acattctggg ggagattgct gtatccatcg tgcgggccct ggagcaccta 540

cacagcaagc tgtctgtgat ccacagagat gtgaagcctt ccaatgtcct catcaacaaa 600

gaggggcacg tgaagatgtg cgactttggc atcagtggct acctggtgga ctctgtggca 660

aagacaatgg atgctggctg caagccttac atggcccctg agaggatcaa ccctgagctg 720

aatcagaagg gctacaacgt caagtctgat gtctggagcc ttggcatcac catgattgag 780

atggccattc tgcgattccc ttacgagtct tgggggacac cgttccagca gctgaagcag 840

gtggtggagg agccatcccc acagctccca gcagaccagt tctcccctga gtttgtggac 900

ttcacaagcc agtgcctcag gaagaatcct gcggaacgca tgagctacct ggagctgatg 960

gaacacccgt tcttcacctt gcacaaaact aagaagacgg acattgctgc ctttgtgaag 1020

gagatcctgg gagaggattc atag 1044

<212> Type : DNA

<211> Length : 1044

SequenceName : 17

SequenceDescription :

Sequence

--------

<213> OrganismName : Ccl2

<400> PreSequenceString :

atgcaggtct ctgtcacgct tctgggcctg ttgttcacag ttgctgcctg tagcatccac 60

gtgctgtctc agccagatgc agttaatgcc ccactcacct gctgctactc attcactggc 120

aagatgatcc caatgagtcg gctggagaac tacaagagaa tcaccagcag caggtgtccc 180

aaagaagctg tagtatttgt caccaagctc aagagagaga tctgtgctga ccccaataag 240

gaatgggtcc agaagtacat tagaaaactg gaccagaacc aagtgagatc agaaactaca 300

gtcttctata aaattgcatc aaccctaagg acttcagcac ctttgaatgt gaacttgacc 360

cataaatctg aagctaatgc atccactctc ttttccacaa ccacctcaag cacttctgta 420

gaagtgacca gtatgacaga gaactag 447

<212> Type : DNA

<211> Length : 447

SequenceName : 18

SequenceDescription :

Sequence

--------

<213> OrganismName : Per1

<400> PreSequenceString :

atgagcggtc ccctagaagg ggctgatggg ggaggagacc ccaggcccgg agaacctttt 60

tgtcctggag gagttccatc ccctggggcc ccacagcatc ggccttgtcc aggccctagc 120

ctggctgatg acactgatgc aaacagcaat ggctcaagtg gcaatgaatc caatggacac 180

gagtccaggg gtgcatctca gcggagttct cacagttcat cttctggcaa tggcaaggac 240

tcagctctgc tggagaccac tgagagcagc aagagtacaa actcacagag cccatcccca 300

cccagcagct ccattgccta tagtctcctg agtgcaagct cagagcagga caacccgtct 360

accagtggct gcagcagtga acagtcagct cgagccagga cccagaaaga actcatgacc 420

gcacttcggg agctcaaact tcggctgcca ccagagcgtc ggggaaaggg ccgctctggg 480

accctggcca cactccagta tgctctggcc tgtgtcaagc aggtgcaggc taaccaggaa 540

tattaccagc agtggagcct ggaggagggt gagccctgtg ccatggacat gtctacttac 600

accctggagg aattggagca tatcacatct gaatacactc tccgcaacca ggataccttc 660

tcggtggctg tgtccttcct gacaggccgg attgtctata tttcggagca ggcaggtgtc 720

ctgctgcgtt gcaaacggga tgtgtttcgg ggtgctcgct tctcagagct cctagctccc 780

caggatgtgg gtgtcttcta tggctctact acaccatctc gcctgcccac ctggggcact 840

ggcacctctg caggttcagg cctcaaggac ttcacccagg aaaagtctgt cttctgccga 900

atcagaggag ggcctgaccg ggatccaggg cctcgctacc agccattccg cctaacccca 960

tatgtgacca agattcgtgt ctcagatggg gcccctgcac agccgtgctg cctgctcatt 1020

gcagagcgca tccactctgg ttatgaagct ccccggattc ctcctgacaa gaggatcttc 1080

accacacggc acacaccaag ttgcctcttc caggatgtag atgaaagggc tgccccactg 1140

ctgggttacc taccccagga tctcctgggg gctccggtac ttctctttct acatcctgag 1200

gaccgacctc tcatgctggc cattcataag aagattctgc agttggcagg ccagcccttt 1260

gaccactccc ctattcgatt ttgtgctcgg aatggggaat atgtcaccat ggataccagc 1320

tgggccggct ttgtgcaccc ctggagccgc aaggtggctt tcgtgctggg ccgccataaa 1380

gtgcgcacgg cacccctgaa tgaggacgtc ttcacacccc cagttcccag tccagctccg 1440

tccctggact ctgatatcca ggagctctca gagcagatcc atcgattgct gctgcagcca 1500

gtgcacagct ccagcaccac ggggctctgt ggagttggcc ctctgatgtc ccctggtcct 1560

ctccacagcc ctggctcctc cagtgatagc aatgggggtg atgctgaggg gcctgggcct 1620

cctgctccag tgactttcca gcagatctgt aaggatgtgc atctggtaaa gcaccaggga 1680

caacagctct tcattgagtc ccgggccaag cctccgcccc ggccccgcct ccttgctaca 1740

ggtacattca aggccaaagt ccttccctgc cagtccccaa acccggaact ggaggtggcc 1800

cctgctcctg accaagcctc attagcattg gcccctgagg agccagagag gaaagagtcc 1860

tctggctgct cctaccagca aatcaactgc ctggacagca tcctcaggta tttggaaagc 1920

tgcaacattc ctaacacaac caagcgtaaa tgtgcctcct cttcctgcac ggcctcttca 1980

gcctctgatg atgacaagca gagggcgggt ccagttcctg tgggggccaa gaaagatacg 2040

tcgtcagcag tgctgtctgg ggagggggca actcctcgga aggagccagt ggtgggaggc 2100

accctgagcc cgctcgccct ggccaataag gcagagagcg tggtgtccgt caccagtcag 2160

tgtagcttca gctccaccat cgtccatgtg ggagacaaga agcccccgga gtcggacatc 2220

atcatgatgg aagacctgcc tggcctggcc cctggtccag cccccagtcc agcccccagc 2280

cccacagtag cccctgaccc agccccagat gcttatcgcc cagtgggtct gaccaaggcc 2340

gtgctgtccc tgcacaccca gaaggaagag caagccttcc tcagccgctt cagagacctc 2400

ggcaggcttc gtgggcttga cacctcttct gtggcgcctt cagcccctgg ttgccaccat 2460

ggccccattc cctctggtcg ccgacaccac tgccgatcta aagcaaagcg ttctcgccac 2520

caccagaccc cccgacccga aaccccctgc tatgtgtctc atccttcacc tgtgccctct 2580

tctggaccct ggccaccccc accagccact acccccttcc cagcagtggt ccagccctac 2640

ccactcccag tgttctcccc tcgaggagga ccccagccgc ttcctcctgc ccccacatct 2700

gtgtcacctg ctaccttccc ttctccctta gtgaccccaa tggtggcctt ggtgctccct 2760

aactatttat tcccttcccc aactagttac ccatatgggg tatcccaggc tcctgttgag 2820

gggccaccta cacctgcttc ccactcacct tctccatccc tacccccacc acctcccagc 2880

cccccccacc gcccagactc cccactgttc aactcaagat gcagttcccc actccagctc 2940

aatctgctgc agcttgagga gtccccccgc actgaagggg gtgctgctgc aggaggcccc 3000

ggaagtagtg ctgggcccct gcctcccagt gaggagtctg ctgagccaga gcccagactg 3060

gtggaggtca cggagtcatc caatcaggat gcactttcag gctccagtga cctgctagag 3120

ttactgctcc aggaggactc tcgctctggc acaggctccg cagcctcggg ctccctgggc 3180

tccggcctgg gctctgggtc tggttcggga tcccacgaag ggggaagcac ctcagccagc 3240

atcactcgca gcagtcagag cagccataca agcaagtact ttggcagcat cgactcttca 3300

gaggctgaag ctggagctgc tcaggccagg actgagcctg gggaccaggt cattaagtat 3360

gtgctccagg atcccatctg gctgctcatg gccaacgctg accagcatgt catgatgacc 3420

taccaggtgc cgtccaggga tgcagcgtct gtgctgaagc aagaccggga gaggctccgg 3480

gccatgcaga aacaacagcc acggttctca gaggaccagc ggcgggaact gggtgctgtg 3540

cactcctggg tccggaaggg ccagctgcct caggccctcg atgtaacggc ttgtgtggac 3600

tgtggtagca gtgttcaaga tcctggccac tcggacgacc ctctcttctc agaactggat 3660

ggattggggc tggagcctat ggaagagggt ggaggcgagg gaggtggtgt tggcgggggt 3720

ggaggtggtg ttggcggggg tgggggtgat ggtggtgagg aggcccagac ccaaattggg 3780

actaagggct caagctctca ggactctgcc atggaggaag aagaacaagg tgggagctct 3840

tccagtccag ctttacctgc agaagagaat ggcaccagct ag 3882

<212> Type : DNA

<211> Length : 3882

SequenceName : 19

SequenceDescription :

Sequence

--------

<213> OrganismName : Lyn

<400> PreSequenceString :

atgggatgta ttaaatcaaa aaggaaagac aatctgaatg acgatggagt agatatgaag 60

actcaaccag tacgtaatac tgaccgaact atttatgtga gagatccaac gtccaataaa 120

cagcaaaggc cagttcctga atctcaactt ttaccaggac agagatttca agcaaaagat 180

ccagaggagc aaggggacat tgtggtggcc ttgtatccct atgatggcat ccacccagac 240

gacttgtctt tcaagaaagg agaaaagatg aaagttctgg aagagcacgg ggagtggtgg 300

aaagctaagt ccctttcatc aaagagagaa ggcttcatcc ccagcaacta cgtggccaag 360

gtcaacacct tagaaaccga agagtggttc ttcaaggaca taacaaggaa agatgcagaa 420

cgacagcttc tggcaccagg gaacagtgcc ggggctttcc tgatcagaga aagtgaaact 480

ttaaagggaa gcttctctct ttccgtcaga gattacgacc ctatgcatgg cgacgtcatt 540

aagcactaca aaatcagaag cctggacaat ggtggttatt acatctctcc gaggatcact 600

tttccctgta tcagtgacat gattaagcat taccaaaagc agtctgatgg tctatgcaga 660

agactggaga aggcatgcat cagtcccaaa ccacagaagc catgggataa agatgcttgg 720

gagatccccc gggagtccat taagctggtg aaaaaactcg gcgcagggca gttcggggaa 780

gtctggatgg gttactataa caacagcaca aaagtggctg taaagaccct caagcctggc 840

accatgtctg ctcaagcatt cctggaagag gccaacctca tgaagacctt gcagcatgac 900

aagctagtgc ggctgtatgc cgtggtcacc aaggaggagc ccatctacat cattaccgag 960

ttcatggcca agggtagctt gctggatttc ctcaagagtg acgaaggcag caaggttctg 1020

ctcccgaaac tcattgactt ttcagctcag attgcagaag gcatggcgta catcgagcgg 1080

aagaactaca tccaccgcga tctgcgagct gccaacgtgc tggtctctga gtctctcatg 1140

tgcaagattg cagactttgg cctcgcgaga gtaatcgaag ataatgaata cacagcgagg 1200

gaaggtgcca agttccctat caagtggacg gctccagagg ccatcaactt cggctgcttc 1260

actatcaagt ctgacgtgtg gtccttcgga attctcctgt acgagatcgt cacctatggg 1320

aagattccct acccagggag aactaacgca gatgtgatga ccgcactgtc acaggggtac 1380

cgaatgccac gaatggagaa ctgtccagat gagctctatg acatcatgaa aatgtgctgg 1440

aaggaatcag cagaggagag gccaaccttt gactacttac agagtgtact ggatgacttc 1500

tacactgcca cggaagggca gtatcagcag cagccatag 1539

<212> Type : DNA

<211> Length : 1539

SequenceName : 20

SequenceDescription :

Sequence

--------

<213> OrganismName : Tlr2

<400> PreSequenceString :

atgctgcaag ctctttggct cttctggatc ttgatggctg tgataggcct ctcaagggaa 60

ggccattctg cccaggcatc tctgtcatgt gatgctgctg gtgtgtgtga tggcagctcc 120

aggtctttca cctctattcc ctcgggactc acagcaaaca caaagaagct tgacctgtct 180

ttcaacaaga tcacctacat tggccatggt gacttgcgag cctgtgtgaa cctccgggtt 240

ctgacattgg agtccagcgg aatcaacaca atagagggag atgcctttta ttctctgggc 300

agtcttgaac acttggactt gtctaataat cacctatcta gtttatcttc ctcctggttc 360

aggccccttt cctctttgaa atacttaaac ttaatgggaa atccttacag gacactgggg 420

gaaacatcac tgttctccaa tctcacaaat ttacaaaacc ttagggtagg aaatgttgac 480

actttcagtg agataaggag aatagatttt gctgggctga cctctctcaa cgaacttgaa 540

attcaggtat taagtctcgg caactatgag tcccgaagtc tacagtcaat tagagacatc 600

tatcacctga ccctgcactt gagcgagtct gctttcctgc tggggatttt tgcagatatt 660

ctgagttccg tgagatattt agaactaaga gatactaact tggctaggtt ccaattttct 720

gaactgtctg tagacgaaat caattcgcca atgaagaagc tggcattccg gaatgcagat 780

ctcaccgata aaagttttaa tgaactcctg aagctgttgc gttacatctt ggaactgatg 840

gaggtggagt ttgatcactg caccctcaat ggggttggta atttcaaccc ctcagagtca 900

gacgtagtga gggagctagg taaagtagaa acggtaacaa tacggagcct gcacatcccc 960

cagttctatt tgttctatga tctgagcact gtgtattccc tcctggagaa agtgaagcga 1020

atcacagtag agaacagtaa ggtctttctg gttccctgct ctttctcaca gcatttaaaa 1080

tcattagagt tcttagacct cagcgaaaat ctgatggttg aagagtattt gaaaaactca 1140

gcctgtgagg gtggctggcc ttctctacaa agcttggttt tgagtcagaa tcatttgaga 1200

tcaatacgaa aaactgctga gattttgctg actctgaaaa acctgacagc ccttgacatc 1260

agcaagaaca gttttcagcc tatgcccgac agttgtcagt ggccaggaaa gatgcgcttc 1320

ctgaacttgt ccagtacagg gatacaggcc gtcaagacat gcattcctca gaccctggag 1380

gtgttggatg ttagtaacaa caatctcgat tcattttctt tgtttttgcc tcggctccaa 1440

gagctgtata tttccagaaa taaactgaag acactcccag aagcttcatt gttccctgtg 1500

ttacaggtca tgaagatcag agagaacgca ataagtactt tctctaaaga ccagcttggt 1560

tcttttccca aactggagac tctggaagca ggtgacaacc atttcatctg ctcctgtgaa 1620

ctcctgtcct tcattctgga gaggccagcc ctggtccatg tcctggttga ctggccagac 1680

agctacctgt gtgattctcc gcctcgcctg catggccaga ggcttcagga tgcccggccc 1740

tcagtcttgg agtgtcacca ggccgcactg gtctctggag tctgctgtgc ccttctcctg 1800

ttgatcctgc tcttaggcgc cctgtgttac catttccatg ggctgtggta tctgagaatg 1860

atgtgggcgt ggctccgggc caagaggaag cccaagaaag ctccttgcag ggacctttgc 1920

tatgatgcct ttgtttccta cagcgagcag gattcctatt gggtggagaa cctcatggtc 1980

cagcagctgg agaactctga cccacccttc aagctgtgtc tccacaagcg ggactttgtt 2040

ccgggcaaat ggatcattga caacatcatt gattccatcg aaaagagcca caaaacagtg 2100

ttcgtgcttt ctgagaactt cgtacgcagt gagtggtgca agtatgaact ggacttctcc 2160

cacttcaggc tctttgacga gaacaatgac gcggccatcc ttgttttgct ggaacccatt 2220

gagaagaaag ccattcccca gcgcttctgc aaactgcgca agataatgaa cactaagaca 2280

tacctggagt ggcccttgga tgaaggccag cgggaagtgt tttgggcaaa tctgagaact 2340

gcaataaaat cctag 2355

<212> Type : DNA

<211> Length : 2355

SequenceName : 21

SequenceDescription :

Sequence

--------

<213> OrganismName : Hdc

<400> PreSequenceString :

atgatggagc ccagtgaata ccgtgaatac caagctagag ggaaagagat ggtggattac 60

atctgccagt acctgagcac cctgcgggag aggcaggtga ccccaaatgt gaagcctggg 120

tacctgcgag cccagatacc ttcaagtgct cctgaggaac ccgacagctg ggatagcatc 180

tttggggaca ttgagcaaat catcatgcct ggggtggttc actggcagag cccccacatg 240

cacgcctact atccggctct cacctcttgg ccatccctgc taggagatat gctggctgac 300

gccatcaact gcttggggtt cacgtgggct tccagcccgg cctgcacaga gctggagatg 360

aacatcatgg actggctggc gaagatgctg gggctcccgg acttcttcct gcaccaccat 420

cccagcagcc aggggggagg cgtcttgcag agcactgtca gcgaatccac tttaattgcc 480

ctgctggcag caaggaagaa caaaatccta gaaatgaaag cgcatgagcc caatgctgat 540

gagtcctctc tgaacgctcg tcttgttgcc tatgcctctg accaggctca ctcttcagtg 600

gagaaggctg gcttgatttc ccttgtgaag atcaaatttc tgcctgtgga tgacaacttc 660

tcactccgag gagaagctct ccagaaggcc atcgaggaag acaagcaaca gggcttggtg 720

cctgtgtttg tctgtgcaac cttagggacc actggagtct gtgcatttga taagctgtca 780

gagctggggc ccatctgtgc cagggaggga ctgtggctcc acgtcgatgc tgcttatgca 840

ggaacagcct ttctgtgccc tgagctccgg ggcttcctga agggcattga gtacgccgac 900

tccttcacct ttaacccttc caagtggatg atggtgcact ttgactgcac tgggttctgg 960

gtcaaggaca agtacaagct acagcagacc ttcagtgtga accccatcta cctcagacat 1020

gcgaactctg gtgtcgccac tgacttcatg cattggcaga tccccttgag ccggcgcttt 1080

cgctccatta agctgtggtt tgtgattcgg tccttcgggg tgaagaatct tcaagcacat 1140

gtcagacacg gtacagacat ggctaaatac tttgaatctc tggtcaggag cgaccctgtt 1200

ttcgaaattc ctgctgagag gcaccttggt ctggtggttt ttcgtctgaa gggtcccaac 1260

tgtctcacag aaagtgtgtt aaaggaaata gccaaaactg gccaggtctt cctcatccca 1320

gccactatcc aggacaagct gatcatccgt ttcaccgtga cgtcccagtt caccaccaag 1380

gatgacatcc tgagagattg gaacctcatc cgagaggctg ctaaccttgt cctgagccag 1440

cactgcactt ctcagccgag ccctcgggcc aagaacctta ttccaccgcc ggtgaccaga 1500

gactccaaag acctgaccaa tgggctatcc ctggagtctg tcaacgaggg aggagatgac 1560

ccagtacagg tccggaagat cttcaggctg ccaggagaca gtctggaaac gacaatggat 1620

ccctttgatg attgcttctc agaagaggcc tccgatacca ccaagcacaa gctgtcgtcc 1680

tttctgttca gttacttgtc gcagaacaag aagaagacaa tgcggtccct cagctgcaac 1740

agtatgccta tgagtgccca gaagtcacct cccccagatg cttccgtgaa gcatgggggc 1800

ttcttccggg ccagaatctt ttctgggttc ccagaagaaa tgatgatgat gaagaaaggt 1860

ggcttcaaaa agctgatcaa gttctacagt gttcccagct ttcctgaatg cagctctcag 1920

tgtggtaccc tccagctgcc ctgctgccct ctgcaggcca tggtgtag 1968

<212> Type : DNA

<211> Length : 1968

SequenceName : 22

SequenceDescription :

Sequence

--------

<213> OrganismName : Il6

<400> PreSequenceString :

atgaagtttc tctccgcaag agacttccag ccagttgcct tcttgggact gatgttgttg 60

acagccactg ccttccctac ttcacaagtc cggagaggag acttcacaga ggataccacc 120

cacaacagac cagtatatac cacttcacaa gtcggaggct taattacata tgttctcagg 180

gagatcttgg aaatgagaaa agagttgtgc aatggcaatt ctgattgtat gaacagcgat 240

gatgcactgt cagaaaacaa tctgaaactt ccagaaatac aaagaaatga tggatgcttc 300

caaactggat ataaccagga aatttgccta ttgaaaatct gctctggtct tctggagttc 360

cgtttctacc tggagtttgt gaagaacaac ttacaagata acaagaaaga caaagccaga 420

gtcattcaga gcaatactga aaccctagtt catatcttca aacaagagat aaaagactca 480

tataaaatag tccttcctac cccaacttcc aatgctctcc taatggagaa gttagagtca 540

cagaaggagt ggctaaggac caagaccatc caactcatct tgaaagcact tgaagaattt 600

ctaaaggtca ctatgaggtc tactcggcaa acctag 636

<212> Type : DNA

<211> Length : 636

SequenceName : 23

SequenceDescription :

Sequence

--------

<213> OrganismName : Prok2

<400> PreSequenceString :

atggaggacc cgcgctgtgc cccgctactg ctacttttgc tgctaccgct gctgctcaca 60

ccgcccgccg gagatgccgc ggtcatcacc ggggcttgcg acaaggactc tcagtgtgga 120

ggaggaatgt gctgcgctgt cagtatctgg gttaagagca taaggatctg cacacctatg 180

ggccaagtgg gagacagctg ccaccccctg actcggaaaa gtcatgttgc aaatggaagg 240

caggaaagaa gaagggcgaa gagaagaaag aggaagaagg aggttccatt ttgggggcgg 300

aggatgcacc acacttgtcc ctgcctgcca ggtttggcat gtttaaggac ttctttcaac 360

cgttttattt gtttggcccg gaagtga 387

<212> Type : DNA

<211> Length : 387

SequenceName : 24

SequenceDescription :

Sequence

--------

<213> OrganismName : Gch1

<400> PreSequenceString :

atggagaagc cgcggggtgt aaggtgcacc aatgggttcc ccgagcggga gctgccgcgg 60

cccggggcca gccgacctgc cgagaagtcc cggccgcccg aggccaaggg cgcacagcca 120

gccgacgcct ggaaggcagg gcggccccgc agcgaggagg ataacgagct gaacctcccc 180

aacctggcgg ccgcttactc gtccatcctg cgctcgctag gcgaggaccc ccagcggcag 240

gggctgctca agacgccctg gagggcggcc accgccatgc agttcttcac caagggatac 300

caggagacca tctcagatgt cctgaacgat gctatatttg atgaggacca tgacgagatg 360

gtgattgtga aggacattga catgttttcc atgtgtgagc atcacctggt cccatttgtg 420

ggaagggtcc atattggtta tcttcctaac aagcaagtcc ttggtctcag caaacttgcc 480

aggattgtgg aaatctacag tagaagacta caagttcaag aacgccttac caaacagatt 540

gcagtggcca tcacagaagc cttgcagcct gctggcgtcg gggtagtgat tgaagcaaca 600

cacatgtgta tggtcatgcg aggtgtgcag aaaatgaaca gcaagactgt cactagcacc 660

atgctaggcg tgttccggga agacccaaag actcgggagg agttcctcac actcatcagg 720

agctga 726

<212> Type : DNA

<211> Length : 726

SequenceName : 25

SequenceDescription :

Sequence

--------

<213> OrganismName : Gyg1

<400> PreSequenceString :

gatcaggctt ttgtgacgct caccacaaac gatgcctatg ccaaaggggc gctggtcctg 60

ggctcatctt tgaaacagca caggactact aggaggacgg ttgtactcgc cagcccacag 120

gtttcagact ccatgagaaa agttttagag actgtctttg atgaagtcat catggtagat 180

gtcttggaca gtggtgattc tgctcatcta accttaatga agaggcctga gttgggcatc 240

acgttgacta aactccactg ctggtcactc actcagtatt ccaaatgtgt gttcatggat 300

gcagatactc tggttctctc aaatattgat gatctttttg aaagagaaga attgtcagca 360

gcaccagacc cagggtggcc tgactgtttc aattctggag tctttgtcta tcaaccctca 420

attgaaacgt ataaccagct gttgcatctt gcttctgagc aaggtagttt tgatggtggg 480

gaccagggcc tactgaatac atattttagt ggctgggcaa cgacagatat cacaaaacac 540

ttgccatttg tgtataacct gagcagcctc tcaatatact cctacctccc ggcatttaaa 600

gcgtttggta aaaatgccaa agttgtgcac ttcctgggac gaaccaagcc atggaattac 660

acgtacaatc cccaaacaaa aagtgtcaaa tgtgaatccc aggacccaat tgtgagtcac 720

ccagagtttc tgaacctgtg gtgggacacc ttcaccacca atgtcttacc cctgctccaa 780

caccatggcc ttgtcaaaga tgccggttcc tatctaatga tggaacatgt cacaggagcc 840

ttgtcagacc tgtcctttgg ggaggcccca cctgcatcac agccttcttt gtcctcagaa 900

gaaaggaagg aacggtggga acagggccag gctgattata tgggagcaga ttcttttgat 960

aacatcaaga gaaaacttga cacttaccta cagtag 996

<212> Type : DNA

<211> Length : 996

SequenceName : 26

SequenceDescription :

Sequence

--------

<213> OrganismName : Myd88

<400> PreSequenceString :

atgtctgcgg gaggcccccg cgtgggatcc gtgtccgtgg actcctactt gttctctcta 60

ccgttggtcg cgctcaacgt gggagtgagg cgccgcctct cgctgttctt gaacccgcgg 120

acaaccgcgg cggccgactg gacctcgctg gcggaggaga tgggtttcga gtacttggag 180

atccgcgagt ttgagacgcg ccccgatccc actcgcagtt tgttggatgc ctggcagggg 240

cgctctggct cgtcggtcgg caggctgcta gagttgctag ccttgttaga ccgtgaggat 300

atactgtatg aactgaagga ccgcatcgag gaggactgcc agaaatacat acgcaaccag 360

cagaaacagg agtctgagaa gcccttgcag gtggccagag tggagagcag tgtcccacag 420

acaaaggaac tgggaggcat caccaccctc gatgaccccc tggggcaaac gccggagctt 480

ttcgacgcct tcatctgcta ctgccccagt gatattgagt ttgtgcagga gatgatccgg 540

caactagaac agacagacta tcggcttaaa ttgtgtgtgt ccgaccgcga tgtcctgcct 600

ggcacctgcg tgtggtccat cgccagcgag ctcattgaga aaaggtgtcg tcgcatggtg 660

gtggttgttt ctgacgatta cctgcaaagt aaggaatgtg acttccagac caagtttgct 720

ctcagcctgt ctccaggtgt ccaacagaag cgactgatcc ctatcaagta caaagcaatg 780

aagaaggact ttcctagtat cctacggttc atcactatct gcgactatac caacccttgc 840

accaagtcct ggttctggac ccgtcttgcc aaggctctgt ccctgccctg a 891

<212> Type : DNA

<211> Length : 891

SequenceName : 27

SequenceDescription :

Sequence

--------

<213> OrganismName : Hdc

<400> PreSequenceString :

atgggcctgg gtgtcctgtg tctggccctt gtcctgcttg gggtcctgca gagccaggcc 60

caggactcaa ctcagaactt gatccctgcc ccacctctga tcagtgtgcc cctgcagcca 120

ggcttctgga ccgaacggtt ccagggcagg tggttcgttg tcggcctggc aggcaatgcg 180

gtccagaaag aaagacaaag ccgctttacc atgtacagca ccatctatga gctacaggaa 240

gacaatagct acaacgtcac ttccatcctc gtcaggggcc agggctgtcg ctactggatc 300

agaacattcg ttccaagctc caggcctggc cagttcaccc tggggaatat tcacagctac 360

cctcagatac agagctacga tgtgcaagtg gccgacactg actacgacca gtttgccatg 420

gtatttttcc agaagacctc tgaaaacaaa cagtacttca aagtcaccct gtacggaaga 480

accaaggggc tgtccgatga actgaaggag cgattcgtca gctttgccaa gtctctgggc 540

ctcaaggata acaacatcgt tttctctgtt cccaccgacc aatgcattga caactga 597

<212> Type : DNA

<211> Length : 597

SequenceName : 28

SequenceDescription :

Sequence

--------

<213> OrganismName : Il1r1

<400> PreSequenceString :

atgctgccga ggcttgtgac atcttcggct aaatcctcca tcctggagaa tatgaaagtg 60

ctacttgggt tcatttgtct cattgtgcct ctgctgtcgc tggagaccga caaatgcacg 120

gagtatccaa atgaggtcat ttcattttca tccgtaaatg aaattgacat tcgcagctgt 180

cctcttactc caaacgaaat gcatggcggc accataatct ggtataaaaa tgacagcaag 240

acccccatat cagcggacaa ggactccagg attcatcagc agaatgaaca tctttggttt 300

gttcccgcca agatggagga ttcaggctat tactattgta taatgagaaa ctcaacatac 360

tgcctcaaaa ctaaaataac catgagtgtc ttagagaatg accctggctt gtgctataac 420

acacaagcca gcttcataca gcggctccat gttgcagggg atggaagtct tgtgtgccct 480

tatctggatt ttttcaaaga tgaaaataac gagttaccca aagttcagtg gtataagaac 540

tgtaaacctc tgcctcttga cgatggaaac ttctttggat tcaagaataa actgatggtg 600

atgaatgtgg ctgaagagca cagagggaac tatacttgcc gcacgtccta cacataccag 660

gggaagcaat atcccgtcac acgagtaata acatttatca caatagatga cagcaagagg 720

gacagacctg tgattatgag cccacggaat gagacgatgg aagctgaccc aggatccacg 780

atacaactga tctgcaacgt cacgggccag ttcaccgacc ttgtctactg gaagtggaat 840

gggtcggaaa ttgaatggga cgatccaatc ctagccgaag actatcagtt tttggaacac 900

ccttcagcca aaagaaagta cactctcatt acaacactta acgtttcaga ggtcaaaagc 960

cagttttatc gctatccgtt catctgcttc gttaagaaca ctcatattct ggagactgca 1020

cacgtacggt tagtataccc agttcctgac ttcaagaatt acctcatcgg gggctttgcc 1080

atcttcacag ctacagccgt gttctgtgct tgcatctaca aagtcttcaa ggttgatata 1140

gtgctttggt acagggactc ctgctctgat tttcttcccc caaaagcttc agatggaaag 1200

acctatgatg cctatgttct ttatcccaag acctacggag agggatcctt tgcatacttg 1260

gatacttttg tgtttaagct gttgccggag gtcttggagg gacagtttgg atacaaactg 1320

ttcatttgtg gaagggatga ctatgttggg gaagatacca tcgaggttac taatgaaaac 1380

gtaaagagaa gcagaaggct gatcatcatt ttagtgagag atatgggaag cttcagctgt 1440

ctgggccagt catctgaaga gcagatagca atatacgatg ccctcatccg ggaaggaatt 1500

aaaatcatcc tgctcgagtt ggagaaaatc caagactatg agaaaatgcc agaatctatt 1560

caattcatta agcagaagca tggggccatt tgctggtcag gagactttaa agaaagaccg 1620

cagtctgcca agaccaggtt ctggaaaaac ttaagatacc agatgccagc ccaacggcga 1680

tcaccactgt ctaaacatca cttactaacc ctggatcctg tgctggacac taaggagaaa 1740

ctgcaggcgg agacacactt accacttggc tag 1773

<212> Type : DNA

<211> Length : 1773

SequenceName : 29

SequenceDescription :

Sequence

--------

<213> OrganismName : Il1rl1

<400> PreSequenceString :

atgattggca aatggagaat ggggctttgg gctttggcaa ttctgacagt tcccatgtat 60

ttcatagtga cagagggcag aaaaacatcc tggggtctag aaaacgaggc tttaattgtc 120

agatgccccc aaagaggagg tgcgattaac cctgtggaat ggtattattc aaatacaaat 180

gaaagaattc ctactcaaaa gagaaatcgg atcttcgtct caagagatcg tctgaagttt 240

ctaccagcca aagtggaaga ctctgggatt tatacgtgtg ttatcagaag ccctgaatcg 300

attaagaccg gatctttgaa tgtcaccata tataaaagac caccaaactg caaaatccct 360

gattacatga tgtactcgac agtagatgga tcagataaaa attccaagat aacatgtcca 420

acaattgcct tgtataattg gacagcgcct gttcagtggt ttaagaactg caaagctctc 480

caagggccaa ggttcagggc acacatgtcc tatttgttca ttgacaaagt gagtcatgtt 540

gatgaaggtg actacacatg tcgattcact cacacggaga acggaaccaa ttacattgtg 600

actgccacca gatcattcac agttgaagaa aaaggcttct ctacatttcc agtaattaca 660

aaccctccac acaactacac agtggaagtg gaaataggaa aaacagcaaa cattgcctgc 720

tcagcttgct ttggcacagc ctctcagttc gttgctgtcc tgtggcagat taacaaaacg 780

agaattggat cttttggcaa agcaagaatt caagaagaga aaggcccaaa taaaaggcat 840

acaaacaatg gcatgatttg cttaacctca ctgttaagga taactggtgt gaccaacaag 900

gacttctccc tgaaatatga ctgtgtggcc atgaaccatc acggagtgat aaggcacccc 960

gtaagactga gaaggaaaca accaattgac caccaaagca cctactacat agttgccgga 1020

tgtagtttat tgctaatgtt tatcaatgtc ttggtgatag tcttaaaagt gttctggatt 1080

gaggttgctc tgttctggag agatataatg gcaccttaca aaacccagaa tgatggaaag 1140

ctctatgatg cttacatcat ttaccctcgg gtcttccggg gcagcgcatc agggaccggc 1200

tctgtggagt actttgttca ctacactctg cccgacgttc tcgaaaataa atgtggctac 1260

aagttgtgca tttacgggag agacctgctg cctgggcaag atgcggccac tgtggtggaa 1320

agcagtatcc agaatagtag acggcaagtg tttgtcctgg cccctcacat gatgcacagc 1380

aaagagtttg cctatgagca ggagatcgcc ctgcacagcg ccctcatcca gaacaactcc 1440

aaggtgattc tgattgaaat ggagcctatg ggtgaggcaa gccgactgca gcttggggat 1500

ctgcaagatt ctctccagca tcttgtgaaa atgcagggga ccatcaagtg gagggaagac 1560

cacgtggccg acaaacagtc tctaagctcc aaattctgga agcatgtgag ataccaaatg 1620

ccagtcccga aaagaccccc caagatggca tctgttgccg ctccgttgag tggcaaggtg 1680

tgcttggacc tgaaacactt ttga 1704

<212> Type : DNA

<211> Length : 1704

SequenceName : 30

SequenceDescription :

Sequence

--------

<213> OrganismName : Tuba1c

<400> PreSequenceString :

atgcgtgagt gcatctccat ccacgttggc caggctggtg tccagatcgg caatgcctgc 60

tgggagctct actgcctgga acatggcatc cagcctgatg gccagatgcc aagtgacaag 120

accattgggg gaggagatga ctccttcaac accttcttca gtgagacagg agctggcaag 180

cacgtgcccc gggcggtgtt cgtagacctg gaacccacag ttattgatga agttcgaact 240

ggcacctacc gccagctctt ccacccagag cagctcatca caggcaagga agatgctgcc 300

aataactatg cccgtggcca ctacaccatt ggcaaggaga tcattgacct tgtcttggac 360

agaattcgca agctggctga ccagtgcacg ggtctccagg gcttcttggt tttccacagc 420

tttggtgggg gaactggctc tgggttcacc tccctgctga tggagaggct ctctgtcgac 480

tacggaaaga agtccaagct ggagttctcc atttacccag ccccccaggt ttccactgct 540

gtggttgagc cctacaattc catcctcacc acccacacca ccctggagca ctctgattgt 600

gccttcatgg tagacaatga ggccatctat gacatctgtc gtagaaacct cgacattgag 660

cgcccaacct acactaacct taaccgcctt attagccaga ttgtgtcttc catcactgct 720

tccctcagat ttgatggggc cctgaatgtt gatctgacag aattccagac caacctggtg 780

ccctaccctc gaatccactt ccctctggcc acttatgccc ctgtcatctc tgctgagaaa 840

gcctaccatg agcaacttac agtagcagag atcaccaatg cctgctttga gccagccaac 900

cagatggtga aatgtgaccc tcgccatggt aaatacatgg cttgctgcct gctgtaccgt 960

ggtgatgtgg tccccaagga tgtcaatgct gccattgcca ccatcaagac caagcgtacc 1020

atccagtttg tggactggtg ccccactggc ttcaaggttg gcattaatta ccagcctccc 1080

actgtggtac ctggtggcga cctggccagg gtgcagagag ctgtgtgtat gctgagcaac 1140

accacagcca ttgctgaggc ctgggctcgc ctggatcaca agtttgatct gatgtatgcc 1200

aagcgtgcct ttgtgcactg gtatgtgggt gagggcatgg aggaggggga gttctctgag 1260

gcccgtgagg atatggctgc cctagagaag gattatgagg aggttggggc agatagtgct 1320

gaaggagacg atgagggtga agaatattaa 1350

<212> Type : DNA

<211> Length : 1350

SequenceName : 31

SequenceDescription :

Sequence

--------

<213> OrganismName : Bambi

<400> PreSequenceString :

atggatcgcc actccagcta catcttcatc tggctgcagt tggagctctg cgccatggct 60

gtgctgctca ccaaaggcga gatcagatgc tactgtgatg ccgctcactg tgtggcaact 120

ggttacatgt gtaagtccga gcttagtgcc tgcttctcca gactccttga tcctcagaac 180

accaattccc cactaaccca cggctgcctg gactcactcg caagcacagc agacatctgc 240

cgagccaaac aggcccaaaa ccactctggt cccaccatgc ccactttgga atgctgtcat 300

gaagacatgt gcaattaccg aggactgcat gatgtcctct ctcctcccaa gagtgaagcc 360

tcaggacaag gaaacagata ccagcatgac agcagcagaa acctcatcac taaggtgcag 420

gagctgactt cctccaaaga actgtggttc cgtgcagccg tgatcgcggt tcccattgct 480

ggcggactga tcttggtgct gctcattatg ttggccttgc gaatgctgcg aagtgagaac 540

aagaggctgc aggatcagcg gcagcagatg ctctctcgtt tgcactacag cttccatgga 600

caccactcca agaagggaca ggttgcaaag ttggacttag agtgcatggt gcccgtcagt 660

gggcaggaga actgctgtct gacctgcgac aaaatgaggc aagcagatct cagcaacgag 720

aagatcctct cccttgtgca ctggggcatg tacagtggtc atgggaagct ggaattcgta 780

tga 783

<212> Type : DNA

<211> Length : 783

SequenceName : 32

SequenceDescription :

Sequence

--------

<213> OrganismName : Slc1a3

<400> PreSequenceString :

atgacaaaaa gcaacggaga agagcccagg atgggaagca ggatggaaag attccagcaa 60

ggggtgcgca agcggacgct cctggccaag aagaaagttc agaacatcac caaggaggat 120

gtgaagagct acctgtttcg gaatgccttt gtgctactca ccgtcagcgc tgtcattgtg 180

ggtacaatcc ttggatttgc cctccgaccg tataaaatga gctaccggga ggtcaagtac 240

ttctcctttc ctggggagct tctgatgcgg atgctgcaga tgttggtctt acccctgatc 300

atctccagtc ttgtcacagg aatggcggcc ctagatagta aggcatctgg gaagatgggg 360

atgcgagctg tggtctatta catgactacc accatcattg ctgtggtgat cggcataatc 420

attgtcatca tcatccaccc cggaaagggc acgaaagaaa acatgtacag agaaggtaaa 480

atcgtgcagg tgactgccgc agatgccttc ctggatttaa tcaggaacat gttcccaccc 540

aatctggtag aagcctgctt taaacagttt aaaaccagct atgagaagag aagctttaaa 600

gtgcccatcc aggccaacga aacactgttg ggcgccgtga tcaacaacgt gtcagaggcc 660

atggagactc tgacccggat ccgggaggag atggtacccg ttcctgggtc tgtgaatggg 720

gtcaatgccc tgggcctcgt tgtcttctcc atgtgcttcg gcttcgtgat cggaaacatg 780

aaggagcagg ggcaagcgct aagagagttc ttcgactctc tcaatgaagc catcatgaga 840

ttggtagcgg tgataatgtg gtatgcacct ctgggcatcc tcttcttgat cgcagggaag 900

attgttgaga tggaagatat gggtgtgatt ggggggcagc ttgccatgta tacagtgaca 960

gtcatcgtcg gcctcctcat tcatgccgtc atcgtcctgc ctctcctcta cttcctggta 1020

acccggaaga acccctgggt tttcattgga gggttgctgc aagcactcat cacagccctg 1080

gggacctcct caagttctgc caccctgccc atcactttca agtgcctgga agaaaacaat 1140

ggtgtggaca aacgcatcac cagatttgtg cttcccgtgg gggccaccat taacatggat 1200

gggaccgccc tctacgaggc tttggccgcc attttcatcg ctcaagttaa caactttgac 1260

ctgaattttg gacagattat aacaataagt atcacagcca cagccgcaag cattggggca 1320

gctggcattc ctcaggccgg tctagtcacc atggtcatcg tgctgacatc tgtgggcctg 1380

cccacggatg acatcacact catcattgca gtggactggt ttctggaccg cctccgaacc 1440

accaccaacg tgctgggtga ctccctcggg gccgggattg ttgaacactt gtcccgacat 1500

gaactgaaga accgagatgt tgaaatgggg aactccgtga ttgaggagaa tgaaatgaaa 1560

aagccgtatc agctgattgc ccaggacaat gaaccagaga aacccgtggc agacagcgaa 1620

accaagatgt ag 1632

<212> Type : DNA

<211> Length : 1632

SequenceName : 33

SequenceDescription :

Sequence

--------

<213> OrganismName : Map3k8

<400> PreSequenceString :

atggagtaca tgagcaccgg aagcgacgag aaagaagaga ttgatttatt aattaaccat 60

ttaaacgtgt cggaagtcct ggacatcatg gagaaccttt atgcaagtga agagcctgca 120

gtgtatgagc ccagtctgat gaccatgtgt ccagacagca atcaaaacaa ggaacattca 180

gagtcgctgc ttcggagtgg ccaggaggtg ccctggttgt cgtctgtcag atatgggact 240

gtggaggatc tgcttgcatt tgcaaaccat atctcgaata cgacaaagca tttttacgga 300

tgtcggcccc aagaatctgg gattttatta aatatggtaa tcagtcccca gaatggtcgc 360

taccaaatcg actcggatgt tctccttgtc ccgtggaagc tgacgtacag gagcattggt 420

tctggtttcg ttcctcgggg ggcctttgga aaagtgtact tagcacaaga catgaagaca 480

aagaaaagaa tggcatgtaa actgatccct gtagatcagt ttaagccatc agatgtggaa 540

atccaggcct gcttccggca cgagaacatt gctgagttat acggtgcggt cctatggggc 600

gacactgtcc atctcttcat ggaagctggc gagggagggt ctgtcctgga gaagctggag 660

agctgtgggc ccatgagaga atttgaaatt atctgggtga caaagcacgt tctcaaggga 720

cttgattttc tgcactccaa gaaagtcatc caccacgata tcaaacctag caacattgta 780

ttcatgtcta cgaaagctgt gttggtagat tttggcctga gtgttcaaat gacagaagat 840

gtctatctcc ccaaggacct ccggggaaca gagatctaca tgagccctga ggtgattctg 900

tgcaggggcc attccacaaa agcagacatc tacagccttg gagccacgct cattcacatg 960

cagacaggca ccccaccctg ggtgaagcgc taccctcgat cggcctatcc ctcctacctg 1020

tacataatcc acaagcaggc acctcccctg gaagatattg ctggtgactg cagtccaggc 1080

atgagggagc tgatagaagc cgccctggag aggaacccca accaccgccc aaaagcagca 1140

gacctactga aacacgaagc cctgaatccc ccaagagagg accagccacg gtgtcagagt 1200

ctggactctg ccctctttga ccggaagagg ctgctgagca ggaaggagct agaacttcct 1260

gagaacattg ctgattcatc atgcacagga agcaccgagg agtctgaagt gctcaggaga 1320

cagcgttccc tctacattga tctcggagct ctggctggct acttcaatat tgttcgtggt 1380

ccaccaaccc tggaatatgg ctga 1404

<212> Type : DNA

<211> Length : 1404

SequenceName : 34

SequenceDescription :

Sequence

--------

<213> OrganismName : Mmp9

<400> PreSequenceString :

atgaacccct ggcagcccct gctcctggtg ctcctggctc taggctacag ctttgctgcc 60

cctcaccagc gccagccgac ttatgtggtc ttcccccgag acctgaaaac ctccaacctc 120

acggacacac agctggcaga ggattacctg taccgctatg gttacactcg ggcagcccag 180

atgatgggag agaagcagtc cctgcggccc gctttgctga tgcttcagaa gcagctgtcc 240

ctgccccaga ctggtgagct ggacagcgag acactaaagg ccattcgttc accgcgctgt 300

ggtgtcccag acgtgggcaa attccaaacc ttcgaaggcg acctcaagtg gcaccatcat 360

aacatcacct attggatcca aagctacacc gaagacttgc cgcgagacgt gatcgatgac 420

tccttcgcgc gcgccttcgc ggtgtggagc gcggtgacac cgctcacctt cacccgcgtg 480

tacgggctcg aagcagacat tgtcatccag tttggtgtcg cggagcacgg ggacgggtat 540

cccttcgacg gcaaggatgg tctactggca cacgcctttc cccctggccc cggcattcag 600

ggagatgccc acttcgacga cgacgagttg tggtcgctgg gcaaaggcgc cgtggtcccc 660

acttactttg gaaacgcaaa tggtgcccca tgtcactttc ccttcacctt cgagggacgc 720

tcctatttgt cctgcaccac ggatggccgc aacgacggca agccttggtg tggcacgaca 780

gctgactacg acacagacag aaaatatggt ttctgcccca gtgagaatct ctacacggag 840

catggcaacg gagacggcaa accctgcgta tttccattca tcttcgaggg ccactcctac 900

tctgcctgca ccactaaagg tcgctcggat ggttatcgct ggtgcgccac caccgccaac 960

tatgaccagg ataagctgta tggcttctgt cctactcgag ccgacgtcac tgtaactggg 1020

ggcaactcgg caggagagat gtgcgtcttc cccttcgtct tcctgggcaa gcagtactct 1080

acctgtaccg gcgagggccg cagtgatggg cgcctctggt gcgcgacgac gtcgaacttc 1140

gacgctgaca agaagtgggg tttctgtcca gaccaagggt acagcctgtt tctggtggca 1200

gcgcacgagt tcggccatgc gctgggctta gatcattctt cagtgccgga agcgctcatg 1260

taccccatgt atcactacca cgaggactcc cctctgcatg aagacgacat aaaaggcatc 1320

cagcatctgt atggtcgtgg ctctaaacct gacccaaggc ctccagccac caccgcagct 1380

gaaccacagc cgacagctcc tcccactatg tgtcccactg cacctcccat ggcctatccc 1440

acagggggcc ccacagtcgc ccctacaggc gccccctcac ctggccccac aggtccccct 1500

actgctggtc cttctgaggc ccctacagag tcttcgactc cagtagacaa tccttgcaat 1560

gtggatgttt ttgatgccat tgctgatatc cagggcgctc tgcatttctt caaggacggt 1620

cggtattgga agttctcgaa tcacggagga agccaattgc agggcccctt tcttattgcc 1680

cgcacgtggc cagctttgcc tgcaaagttg aactcagcct ttgaggatcc gcagtccaag 1740

aagattttct tcttctctgg gcgcaaaatg tgggtgtaca caggccagtc ggtgctgggc 1800

cccaggagtc tggataagtt gggtctaggc tcagaggtaa ccctggtcac cggacttctc 1860

ccgcgtcgtg gagggaaggc tctgctgatc agccgggaac gtatctggaa attcgacttg 1920

aagtctcaga aggtggatcc ccagagcgtt actcgcttgg ataacgagtt ctctggcgtg 1980

ccctggaact cacacaacgt ctttcactac caagacaagg cctatttctg ccatgacaaa 2040

tacttctggc gtgtgagttt ccacaaccgg gtgaaccagg tggaccacgt ggcctacgtg 2100

acctatgacc tcctgcagtg cccttga 2127

<212> Type : DNA

<211> Length : 2127

SequenceName : 35

SequenceDescription :

Sequence

--------

<213> OrganismName : Nedd4l

<400> PreSequenceString :

tttccttgca gtgacccata cgtgaagctc tcgttgtacg tagctgacga gaacagagaa 60

ctcgctctgg ttcagactaa gacaattaaa aagacgctga acccaaagtg gaatgaagag 120

ttttatttta gagtaaatcc atctaatcac aggctcttat ttgaagtatt tgacgagaac 180

agattgacac gagacgactt tctgggccag gtggacgtgc ctcttagtca ccttccgaca 240

gaagatccaa ccatggagag accctataca tttaaggatt ttctcctgcg acctagaagt 300

cataaatcac gcgtcaaggg gtttttgagg ttgaaaatgg cctacatgcc gaaaaacgga 360

ggtcaggatg aagaaaacag cgagcagagg gacgacatgg agcatggatg ggaagttgtt 420

gactcaaacg actcagcttc ccagcaccag gaggagctcc ctcctcctcc cctgccacca 480

ggatgggaag agaaagtgga caatttaggc cgaacttact atgtcaacca caacaacagg 540

agtacgcagt ggcaccgacc tagcttgatg gatgtatcgt cagagtcaga caataacatc 600

aggcagatca atcaggaggc cgcacaccgg cgtttccgct ctcggaggca cattagtgaa 660

gacttggagc ctgaggcctc tgaaggtggt ggagaaggcc ctgagccttg ggagaccatt 720

tcagaggaag tgaacatggc gggagattct ctcagcctgg ctctaccccc acctcctgcc 780

tccccagtgt caaggaccag cccccaggag ctgtcggaag aactgagcag aaggttgcag 840

atcactccgg actccaatgg ggaacagttc agtgctctga ttcagagaga gccctcgtct 900

aggctccggt cctgcagtgt taccgacgcg gttgctgaac aagctcacct tccaccgccc 960

agcaccccaa ctaggcgagc ccgttcgtca actgtcacgg gtggtgagga atcaacgcca 1020

tcagtggcct atgtacatac cacgccgggc ctaccttcag gctgggaaga aagaaaagat 1080

gctaagggac gcacatacta tgtcaatcat aacaatcgaa ccacaacttg gactcgacca 1140

atcatgcagc ttgcagaaga cggtgcctct ggatcagcca caaacagtaa caaccaccta 1200

gtcgaacccc agatccgccg gcctcgtagc ctcagctcgc caacagtaac cttatctgcc 1260

ccactggagg gtgccaagga ttcacccata cgccgtgctg tgaaagatac cctttccaat 1320

ccacagtccc ctcagccatc accttacaac tcccccaaac cacaacacaa agtcacacag 1380

agcttcctgc caccaggctg ggagatgagg atagcgccaa acggccgacc cttcttcatt 1440

gaccataaca caaagacgac aacctgggaa gatccacggc tgaaatttcc agtacatatg 1500

cggtcaaaag catctttaaa ccccaatgac ctgggccctc ttcctcctgg ctgggaagaa 1560

aggatccacc tggacggccg cacattttac attgaccata atagcaaaat tactcagtgg 1620

gaagatccaa gactacagaa tccagccata actggtccgg ctgtgccgta ctccagagaa 1680

tttaagcaga aatacgacta ctttaggaag aaattaaaga aacctgctga tattccaaac 1740

aggtttgaaa tgaaactcca cagaaacaac atatttgaag aatcctatcg gaggatcatg 1800

tctgtaaaga gacctgacgt cctaaaggct agactgtgga tcgagtttga atcagagaaa 1860

ggcctggact atgggggcgt ggccagagag tggttcttct tactgtccaa agagatgttc 1920

aatccctact atggcctctt cgagtactct gccacggaca actacacact tcagatcaat 1980

cccaactcag gcctgtgtaa cgaagatcac ttatcctatt tcactttcat tgggagagtt 2040

gctggcctgg cagtgtttca tgggaaactc ttagatggat tcttcattcg acctttctac 2100

aagatgatgc tggggaagca aataactctg aatgacatgg agtctgtgga cagcgagtac 2160

tacaactctt tgaagtggat cttggaaaac gaccccacgg aacttgacct catgttctgc 2220

atagatgaag agaactttgg gcagacttac caagtggacc tgaagcccaa tgggtcagaa 2280

atcatggtaa ccaatgagaa caagcgagaa tatattgact tggtcatcca gtggagattt 2340

gtgaacaggg tccagaagca aatgaatgcc ttcttggagg gatttacaga acttcttcca 2400

atcgacttga ttaaaatttt tgatgaaaat gagctggagt tgctgatgtg cggccttggt 2460

gatgtggacg tgaatgattg gagacagcac tccatttaca agaacggcta ctgccccaac 2520

caccctgtca tccagtggtt ctggaaggcc gtgctgctga tggatgctga gaagcggatc 2580

cggttactac agtttgtcac aggcacctcc agagtaccca tgaatggatt tgccgaactt 2640

tatggttcca atggtcctca gctgtttaca atagagcaat ggggtagtcc tgaaaaactg 2700

cccagagctc atacatgctt taaccgcctt gatttacctc cgtatgaaac ctttgaagat 2760

ctacgggaga agcttctcat ggccgtggag aatgctcaag gcttcgaagg tgtggattaa 2820

<212> Type : DNA

<211> Length : 2820

SequenceName : 36

SequenceDescription :

Sequence

--------

<213> OrganismName : Cd14

<400> PreSequenceString :

atgaagctta tgctcggctt gttgctgttg cctttgacac tggtgcatgc ctctcccgcc 60

acaccagaac cctgtgagct ggaccaggac gaggaaagtg tccgctgcta ctgcaacttc 120

tcagatccgc agcccaattg gtccagcgct tttctatgcg cgggggcgga agacgtggag 180

ttctacggcg gcggtcgcag cctggagtac cttctaaagc gtgtggacac ggaagcaaat 240

ctggggcagt acactgatat tatcaggtct ctgcccttga agaggcttac tgtgcggtcc 300

gcgcgggttc ctactcagat tctattcgga accctgcgtg tgctcggata ttctggcctc 360

cgggaactga ctcttgaaaa cctcgaggta accggcaccg cgctttcgcc gcttctggat 420

gccaccggac ccgatctcaa caccttgagc ctccgcaacg tgtcgtgggc aacaacggat 480

acctggctcg cagaactgca gcagtggcta aagcctggac tcaaggtact aagtattgcc 540

caagcacact cactcaactt ttcttgcaaa caggtcggcg tcttccctgc ccttgccacc 600

ttagatctgt ctgacaaccc tgagttgggc gagaaaggac tgatctcggc cctctgtccg 660

cacaaattcc cgaccctcca agttttagcg ctgcgcaacg cggggatgga gacgaccagc 720

ggcgtgtgct ctgcgctggc cgcagcaaga gtgccactgc aggcactgga tctcagtcac 780

aattcactgc gggatactgc tggcactccg agctgtgact ggcccagtca gctaaactcg 840

ctcaatctat ctttcactgg gctggagcac gtacctaaag ggctgccagc caagcttagc 900

gtgcttgatc tcagttacaa caggctggat aggaaaccta ggccagagga gctgcctgaa 960

gtggggagcc tgtcacttac aggaaatccc tttttgcact ctgaatccca gtcggaggcg 1020

tataactctg gcgtagtcat agccacagct ctttcacccg gatcagcggg cttgtcagga 1080

actttggctt tgctcctagg acatcgcctc tttgtttaa 1119

<212> Type : DNA

<211> Length : 1119

SequenceName : 37

SequenceDescription :

Sequence

--------

<213> OrganismName : Sod2

<400> PreSequenceString :

atgttgtgtc gggcggcgtg cagcgcgggc agaagactgg gccccgcggc cagtaccgcg 60

ggctcccggc acaagcacag cctccctgac ctgccttacg actatggcgc gctggagccg 120

cacattaacg cgcagatcat gcagctgcac cacagcaagc accacgcgac ctacgtgaac 180

aatctgaacg tcaccgagga gaagtaccac gaggcgctgg ccaagggaga tgttacaact 240

caggttgctc ttcagcctgc actgaagttc aatggcgggg gccatatcaa tcacagcatt 300

ttctggacaa acctgagccc taagggtggt ggagaaccca aaggagagtt gctggaggct 360

atcaagcgtg actttgggtc ttttgagaag tttaaggaga aactgacagc tgtgtctgtg 420

ggagtccaag gttcaggctg gggctggctt ggcttcaata aggagcaagg tcgcttacag 480

attgccgcct gctctaatca ggacccactg caaggaacca caggccttat tccactgctg 540

gggattgatg tgtgggagca cgcttactat cttcagtata aaaacgtcag acctgactat 600

ctgaaagcca tttggaatgt aatcaactgg gagaatgtta gccaaagata catagtttgc 660

aagaagtga 669

<212> Type : DNA

<211> Length : 669

SequenceName : 38

SequenceDescription :

Sequence

--------

<213> OrganismName : Acsl6

<400> PreSequenceString :

atgctgacct tcttcttggt gtcggggggt tccctctggc tgtttgccga gattgccctg 60

tcacttctgg agaagatgca gacccaggag atcctgagga tcctgcggct gcccgagcta 120

tcggacttgg gccagttttt ccgcagcctc tcagccacca cccttgtgag tatgggtgca 180

ctggctgcca tccttgcata ctggctcact caccggccaa aggccttgca accaccatgc 240

aacctcctga tgcagtcgga agaagtagag gacagtggtg gagcccggcg atctgtgatt 300

ggggattgca ctcagttgct tacccactac tatgacgatg cccggacgat gtaccaggtg 360

ttccgacgtg ggcttagcat ctcagggaat ggaccctgtc ttggcttccg gaagccagag 420

caaccttacc agtggctgtc ctatcaagag gtggccaaaa gggctgaatt cctggggtca 480

ggacttctcc agcatgactg taaagtgggc acagagcagt tcattggcgt ttttgcacaa 540

aatcggccag agtggatcat tgctgagctg gcctgctaca catattccat ggtggtggta 600

ccgctctatg acacactggg cccagggtct atccgctaca tcatcaatac agcggacatc 660

tgcactgtaa tcgtggataa gccgcataag gctatactcc tgctagaaca cgtggagagg 720

aaggagactc cgggcctcaa gctggtcatc ctcatggagc catttgacga tgccctgaga 780

gagaggggaa agaagtgcgg ggtggacatc aagtccatgc aggctataga ggactccggc 840

caagagaatc atcgtgtccc cgtgccccca cggcccgatg acctctccat tgtgtgtttc 900

acgagcggta caacagggaa ccccaaaggt gcgatgctca cccacgggaa cgtggtggcc 960

gatttctcgg gctttctgaa agtcacagag agtcagtggg cccccacttg tgcggatgta 1020

cacttttcct atttgccttt agcacacatg tttgagcgaa tggtgcagtc tgttgtctac 1080

tgccatggag ggcgtgtggg cttcttccag ggagacatcc gcctcctctc agacgacatg 1140

aaagctctcc gccccaccat cttccctgtg gtcccacggc tgctgaatcg gatgtatgac 1200

aagatcttcc accaggcaga cacctcactg aagcgctggc tcctggagtt tgcagcaaag 1260

cgcaagcagg ctgaggtccg gagcggaatc atcagaaaca atagtatctg ggatgaactc 1320

ttctttaata agattcaggc cagtcttgga gggcacgtga gaatgattgt taccggagca 1380

gcccccgcat caccaacagt cctgggattc ctacgagcag ctctggggtg ccaggtctat 1440

gaaggttatg ggcaaactga atgcacagct gggtgtacct tcacaacgcc aggggactgg 1500

acatcagggc acgtgggagc ccctctgccc tgcaaccaca tcaagctggt cgacgcagag 1560

gaactcaact actggaccag caaaggagaa ggagagatct gtgtgaaagg accaaatgtg 1620

ttcaaaggct acttaaaaga cgaggacagg acaaaggagg ccctggacag tgacggctgg 1680

cttcacacgg gagacattgg aaaatggctg ccggagggaa cgcttaaaat catcgatcgg 1740

aaaaagcaca tatttaaact tgctcagggg gaatacgttg cgcccgagaa gattgagaac 1800

atctacatcc gaagtgagcc tgtggcgcaa atctacgtcc atggggacag cttaaaggcc 1860

tttctggttg gcattgttgt gcctgaccct gaagtcatgc cttgctgggc tcagaagaaa 1920

ggaatcgaag ggaactacca ggaactctgc aaaagtaagg aattgaagaa agccattctg 1980

gatgacatgg tgatgttggg gaaagaaagt ggactgcatt cttttgaaca ggttaaagcc 2040

atttacatcc attgtgacat gttctcagtt caaaatggct tgctgacacc aacactaaag 2100

gctaagaggc ccgagctgag agagtacttc aaaaagcaaa tagaagagct gtactcaatc 2160

tccatgtga 2169

<212> Type : DNA

<211> Length : 2169

SequenceName : 39

SequenceDescription :

Sequence

--------

<213> OrganismName : Alox12

<400> PreSequenceString :

atgggccgct accgcgtccg cgtggtcact ggggcctggc tcttctcggg atccgtcaac 60

ctggtgcgtc tgtggctggt cggggcgcac agggaggcga ggctggagct gcagctgcgg 120

ccagcgcggg gcaaggagga ggagtttgat ttcgatgttg ccgaggactt ggggccactg 180

cagttcgtga aactgcacaa agagcacaca gttgtggacg acgcctggtt ctgcaacctc 240

atcacagttc aggggcccgg gatgagcgca gaggccgtct tcccctgcta ccgctgggtg 300

cagggagaag gaatcctgag actgccggag gggacagccc gtctggcagg agacaatgcc 360

ttggatgtct tccagaagca tcgagagaag gaactgaagg aaagacaaca gacctaccgc 420

tgggccacct ggaagcaagg cttacctcag acaatagcag cagactgtaa ggatgacctc 480

cctccaaaca tgagattcca tgaggagaag agactggact ttgaatggac gctgaaggcg 540

ggggttctgg aaatgggcct caaacgtgtt tataccctcc tgagaagctg gaaccgtctg 600

gaagactttg atcagatctt ctggggccag aagagtgcct tggctgagaa ggttcaccgg 660

tgttggcagg aagatgagct ctttggctac cagtttctca atggcgccaa ccccatgctg 720

ttgagacgct ccacctctct gccctccaga ctggtactgc cccctgggac agaggagctt 780

caagcgcagc tggagaaaga actcaaggat ggatgcctgt ttgaggctga ctttatcctg 840

ctggatggaa ttccagctaa tgtgatccga ggagaacagc agtacctggc tgcccctctt 900

gtcatgttga ggatggaccc cagtgggaag ctgctcccca tggctatcca gattcagcct 960

cccaatccca gctccccagc tccaacattg ttcctgccct cagatcctcc acttgcctgg 1020

ctcttggcta agatctgggt ccgaaattca gatttccaac tgcaggagct ccaattccat 1080

ttgctgaaca ctcatctggt ggctgaggtc attgctgttg ccaccatgag gtgcctccca 1140

ggactacacc ctatctttaa gctcctagtt ccgcacatcc gttacactat ggaaattaac 1200

acacggagcc ggacccagct catctcagat ggagggatat ttgatcaggt agtgagcaca 1260

ggtggaggcg gccatgttca gttgctcact cgggcagtgg ctcagctgac gtactgctcc 1320

ctctgtcctc ccgatgatct ggctacccga ggcctgctga gagtcccaag tgccctctac 1380

gctcaggatg ctttgcagct ctgggaggtc actgccaggt atgtgaacgg gatggtccat 1440

ctcttctacc agagtgacga tattgtgagg ggagacccag agctgcaggc ctggtgtcgg 1500

gagatcactg aggtggggct gtgccatgcc caggacagag gattccctgt ctccttccag 1560

tcccgggcac agctctgcca tttccttacc atgtgtgttt tcacatgtac ttcccagcat 1620

gctgccatca accagggcca gctggactgg tacggttggg tacctaatgc tccatgcaca 1680

atgaggatgc ctccaccaac cagcaaggac gatgtgacga tggagaccat catgggctca 1740

ctccctgatg ttcagaagtc ctgtctgcag atgaccatca catggcacct gggccgcctc 1800

cagccagaca tggtacctct aggacaccac aaagaaaagt atttctcaga cccaaggacc 1860

aaagctgtgc tcagccaatt tcaagcagat ctggaaaatt tggagaaaga gattacagcc 1920

cggaaccagc agcttgacct gccctacgaa tacctcaagc ctagccgtat agagaacagc 1980

atcaccatct ga 1992

<212> Type : DNA

<211> Length : 1992

SequenceName : 40

SequenceDescription :

Sequence

--------

<213> OrganismName : Gria2

<400> PreSequenceString :

atgcaaaaga ttatgcatat ttctgtcctc ctttctcctg ttttatgggg actgattttt 60

ggtgtctctt ctaacagcat acagataggg gggctatttc caaggggcgc tgatcaagaa 120

tacagtgcat ttcgggtagg gatggttcag ttttccactt cggagttcag actgacaccc 180

catatcgaca atttggaggt agccaacagt ttcgcagtca ccaatgcttt ctgctcccag 240

ttttcaagag gagtctacgc aatttttgga ttttatgaca agaagtctgt aaataccatc 300

acatcattct gtgggacact ccatgtgtcc ttcatcacac ctagcttccc aacagatggc 360

acacatccat ttgtcatcca gatgcgacct gacctcaaag gagcactcct tagcttgatt 420

gagtactacc aatgggacaa gttcgcatac ctctatgaca gtgacagagg cttatcaaca 480

ctgcaagctg ttctggattc tgctgcagag aagaagtggc aggtgactgc tatcaatgtg 540

gggaacatca acaatgacaa gaaagatgag acctacagat cgctctttca agatctggag 600

ttaaaaaaag aacggcgtgt aatcctggac tgtgaaaggg ataaagtaaa tgacattgtg 660

gaccaggtta ttaccattgg aaaacatgtt aaagggtacc attatatcat tgcaaatctg 720

ggattcactg atggggacct gctgaaaatt cagtttggag gagcaaatgt ctctggattt 780

cagattgtag actacgacga ttccctggtg tctaaattta tagaaagatg gtcaacactg 840

gaagagaaag aataccctgg agcacacaca gcgacaatta agtatacttc ggccctgacg 900

tatgatgctg tccaagtgat gactgaagca ttccgtaacc ttcggaagca gaggattgaa 960

atatcccgga gaggaaatgc aggagattgt ttggccaacc cagctgtgcc ctggggacaa 1020

ggggtcgaaa tagaaagggc cctcaagcag gttcaagttg aaggcctctc tggaaatata 1080

aagtttgacc agaatggaaa acgaataaac tacacaatta acatcatgga gctcaaaaca 1140

aatggacccc ggaagattgg gtactggagt gaagtggata aaatggttgt caccctaact 1200

gagctcccat caggaaatga cacgtctggg cttgaaaaca agactgtggt ggtcaccaca 1260

ctattggaat ctccatatgt tatgatgaag aaaaatcatg aaatgcttga agggaatgag 1320

cgttacgagg gctactgtgt tgacttagct gcagaaattg ctaaacactg tgggttcaag 1380

tacaagctga ctattgttgg ggatggcaag tatggggcca gggatgccga caccaaaatt 1440

tggaatggta tggttggaga gcttgtctac gggaaagctg acattgcaat tgctccatta 1500

actatcactc tcgtgagaga agaggtgatt gacttctcca agcccttcat gagtcttgga 1560

atctctatca tgatcaagaa gcctcagaag tccaaaccag gagtgttttc ctttcttgat 1620

cctttagcct atgagatctg gatgtgcatt gtgtttgcct acattggggt cagtgtagtt 1680

ttattcctgg tcagcagatt tagcccctac gagtggcaca ctgaggaatt tgaagatgga 1740

agagaaacac aaagtagtga atcaactaat gaatttggga tttttaatag tctctggttt 1800

tccttgggtg cctttatgca gcaaggatgc gatatttcgc caagatccct ctctgggcgc 1860

attgttggag gtgtgtggtg gttctttacc ctgatcataa tctcctccta cacggctaac 1920

ttagctgcct tcctgactgt agagaggatg gtgtctccca tcgaaagtgc tgaggatctg 1980

tctaagcaaa cagaaattgc ttatggaaca ttagactctg gctccactaa agagtttttc 2040

aggagatcta aaatcgcagt gtttgataaa atgtggactt atatgaggag tgcagagccc 2100

tctgtgtttg tgaggactac cgcagaagga gtagccagag tccggaaatc caaaggaaag 2160

tatgcctact tgctggagtc cacaatgaac gagtacatcg agcagaggaa gccttgtgac 2220

accatgaaag tgggaggaaa cttggattcc aaaggctacg gcatcgccac acctaaagga 2280

tcctcattaa gaaccccagt aaatcttgca gtattgaaac tcagtgagca aggcgtctta 2340

gacaagctga aaaacaaatg gtggtacgat aaaggtgaat gtggagccaa ggactcggga 2400

agtaaggaaa agaccagtgc cctcagtctg agcaacgttg ctggagtatt ctacatcctt 2460

gtcgggggcc ttggtttggc aatgctggtg gctttgattg agttctgtta caagtcaagg 2520

gccgaggcga aacgaatgaa ggtggcaaag aatccacaga atattaaccc atcttcctcg 2580

cagaattccc agaattttgc aacttataag gaaggttaca acgtatatgg catcgagagt 2640

gttaaaattt ag 2652

<212> Type : DNA

<211> Length : 2652

SequenceName : 41

SequenceDescription :

Sequence

--------

<213> OrganismName : Ncam1

<400> PreSequenceString :

tttttttttt cttttgcagt ttccctgcag gtagatattg ttcccagcca aggagaaatc 60

agcgttggag agtccaaatt cttcctgtgt caagtggcag gagatgccaa agataaggac 120

atctcctggt tctcccccaa cggggagaaa ctgagcccaa accagcagcg gatctcagtg 180

gtgtggaacg atgatgactc ctctaccctc accatctaca acgccaacat tgatgatgcc 240

ggcatttaca agtgcgtggt caccgctgaa gacggcaccc agtccgaggc cactgtcaat 300

gtgaagatct tccagaagct catgttcaag aatgcgccaa ccccacagga gtttaaggaa 360

ggggaagatg ctgtgattgt ctgtgatgtg gtcagctctc tgcccccaac catcatctgg 420

aaacacaaag gccgagatgt catcctgaaa aaagatgtcc ggttcatagt cctatccaac 480

aactacctgc agatccgagg catcaagaaa acagatgagg gcacttaccg ctgtgaaggc 540

aggatcctgg cccgggggga gatcaacttc aaggacattc aggtcattgt gaatgtacca 600

cccactgtcc aggccagaca gagcatcgtg aatgccactg ccaacctggg ccagtctgtc 660

accctggtgt gtgatgccga tggcttccca gagcccacca tgagctggac aaaggatggg 720

gaacccatag agaatgagga ggaagatgac gagaagcaca tcttcagtga cgacagttcg 780

gagctgacca tcaggaatgt ggacaaaaat gacgaagccg aatacgtctg catcgctgag 840

aacaaggctg gcgagcagga tgcctccatc cacctcaagg tcttcgcaaa gcccaaaatc 900

acctatgtag agaatcagac agccatggaa ctagaggagc aagtcactct gacatgtgaa 960

gcctccggag accccattcc ttccatcacc tggagaacgt ccacccgaaa catcagcagt 1020

gaagaaaagg catcgtggac tcgaccagag aagcaagaga ctctagatgg gcacatggtg 1080

gtacgcagcc atgctcgtgt gtcctccctg accctgaaga gcatccagta cacagatgct 1140

ggagaataca tctgcactgc cagcaacacc atcggccagg actcccagtc catgtacctt 1200

gaagttcaat atgctcccaa gctccagggc cctgtagctg tgtacacttg ggaagggaac 1260

caggtgaaca tcacctgtga ggtctttgcc tacccaagtg ccacaatctc ctggttccga 1320

gatggccagt tgctgccaag ctccaactac agcaatatca agatctacaa caccccatct 1380

gcgagctatc tggaggtaac ccctgattcc gaaaatgact ttggaaacta caactgcaca 1440

gcggtgaacc gtattggaca ggagtccttg gaattcatcc tggttcaagc agatacacca 1500

tcttccccat ccatcgaccg ggtggaacca tactccagca cagcacaggt acaatttgat 1560

gagccagaag ccacaggtgg agttcccatc ctcaaataca aggctgagtg gaagtcgctg 1620

ggtgaagaag catggcattc caagtggtat gatgccaaag aagccaacat ggaagggatt 1680

gtcaccatca tgggcctgaa gcctgagaca aggtacgcgg tacgactggc ggccctcaac 1740

ggcaaggggc tgggcgagat cagtgcagcc actgagttca agacacagcc agtccgggaa 1800

cccagcgcac ccaagctgga agggcagatg ggagaggacg ggaactccat caaggtgaac 1860

ctgatcaagc aggatgacgg cggctccccc atcagacact atctggtcaa gtacagagcg 1920

ctcgcctccg agtggaaacc agagatcagg ctcccgtccg gcagtgacca cgtcatgctc 1980

aagtccctag actggaacgc cgagtacgaa gtatatgtgg tagctgagaa ccagcaagga 2040

aaatccaagg cagctcactt cgtgttcagg acttcagccc agcccacggc catcccagcc 2100

aatggcagcc ccactgcagg cctgagcaca ggcgccattg tgggcatcct cattgtcatt 2160

ttcgtcctac tcctggtggt catggacatc acctgctact tcctgaacaa gtgtggcctg 2220

ctcatgtgca tcgctgttaa cctgtgcggc aaagcggggc ccggagccaa gggcaaagac 2280

atggaggagg gcaaggctgc tttctcgaaa gatgagtcta aagaacccat tgtagaggtc 2340

cgaacggagg aggaacggac tccaaaccat gacggaggga agcacacaga gcccaacgag 2400

accacaccac tgacagagcc cgagaagggt cctgtagaaa caaagtccga gccccaggag 2460

tcagaagcaa agccagcgcc aactgaagtc aagacggtcc ccaatgaagc cacacaaacg 2520

aaagagaatg agagcaaagc atga 2544

<212> Type : DNA

<211> Length : 2544

SequenceName : 42

SequenceDescription :

Sequence

--------

<213> OrganismName : Gpr84

<400> PreSequenceString :

atgtggaaca gctcagatga caacttctcc tgctaccatg agtctgtatt gggctatcga 60

tactttgcag ttatctgggg catggtagtg gctgcaacag gcaccgtggg caatgtgctc 120

accctgttgg ccttggccat ccgtcccaaa ctccgaaccc gtttcaacct gctcattgcc 180

aacctcaccc tggctgatct actctactgc acgctcctgc agcctttctc cgtggacaca 240

tacctccacc tccattggcg caccggcgcc atcttctgta gaatattcgg actcctcctc 300

tttacttcca attctgtctc cattcttacc ctctgtctca ttgctctagg acgctacctt 360

ctcattgccc accctaagct ctttccccag gttttcagtg ccaaggggat cgtgctggca 420

ctagtgggca gctgggttgt gggggtgacc agctttgccc ccctctggaa tgtttatgtc 480

ttggtgccag ttgtctgcac ctgcagcttt gaccgcgtgc gaggccggcc ttacaccacc 540

atcctcatgg gcatcttctt tgtggttggg ctcagcagcg tgggcgtctt ctactgcctc 600

atccaccgcc aagtgaagcg tgcggctcga gcgctggaca aatatgggct gcaggaggcc 660

agcatgcgct cccatcaggt gtctgggaca catgaagctg tgccaggcca cttccaggag 720

ctagacagcg ggcttgcatc aagaggtccc agcgaaggga tttcatctga gccagtcagt 780

gctgcgacga cacagaccct ggaaggtgat tcgtcagaag cgggggacca gggcatgaga 840

aaggcagctc agcagatctc agagagaagc cttccagaag tgcatcgcaa gactggagga 900

gctgcaggag cacgcagagc cacggatgca ccatcggagt tcgggaaggt gacccgtatg 960

tgctttgcag tgttcctttg cttcgtcctc agctacatcc ctttcctgct gctcaacatt 1020

ctggacgcca ggggccgcgc tccacgagta gtgcatatgg ttgctgccaa cctcacctgg 1080

ctcaacagct gcatcaaccc tgtgctctat gcagccatga accgccagtt tcgccaggct 1140

tatggctcca tcctgaaacg cgggccacag agtttccgac ggttccatta g 1191

<212> Type : DNA

<211> Length : 1191

SequenceName : 43

SequenceDescription :

Sequence

--------

<213> OrganismName : C3

<400> PreSequenceString :

atgggaccca cgtcagggtc ccagctacta gtgctactgc tgctgttggc cagctccctg 60

ctagctctgg ggagccccat gtactccatc attactccca atgtcctgcg gctggagagt 120

gaagagactt tcatactaga ggcccatgat gctcagggtg atgtcccagt cactgtcact 180

gtgcaagact tcctaaagaa gcaagtgctg accagtgaga agacagtgtt gacaggagcc 240

actggacatc tgaacagggt ctccatcaag attccagcca gtaaggaatt caatgcagat 300

aaggggcaca agtacgtgac agtggtggca aacttcgggg caacagtggt ggagaaagcg 360

gtgctagtaa gctttcagag tggttacctc ttcatccaga cagacaagac catctacacc 420

ccaggctcca ctgttttcta tcggatcttc actgtggaca acaacctatt gcctgtgggc 480

aagacagtcg tcatcgtcat tgagaccccg gacggcgttc ccatcaagag agacattcta 540

tcttcccaca accaatatgg catcttgcct ttgtcttgga acattccaga actggtcaac 600

atggggcagt ggaagatccg agccttctat gaacatgcac caaagcagac cttctctgca 660

gagtttgagg tgaaggaata cgtgctgccc agtttcgaag tcctggtgga gcctacagag 720

aaattttatt acatcgatga cccaaagggc ctggaagttt ccatcacagc cagattcctg 780

tatgggaaga acgtggacgg gacagctttc gtgatctttg gggtccagga tgaggataag 840

aagatttctc tggcccagtc cctcacccgc gtgctgatcg aggatggttc aggggaggca 900

gtgctcagcc gaaaagtgct gatggacggg gtacggccct ccagcccaga agccctagtg 960

gggaagtccc tgtacgtctc tgtcactgtt atcctgcact caggtagcga catggtagag 1020

gcagagcgca gtgggatccc aattgtcact tccccgtacc agatccactt caccaagaca 1080

cccaaattct tcaagccagc catgcctttc gacctcatgg tgtttgtgac caaccctgat 1140

ggctctccag cccgcagagt gccagtagtc actcagggat ccgacgcgca ggctctcacc 1200

caggatgatg gtgtggccaa gctgagcgtc aacacaccca acaaccgcca acccctgact 1260

atcacggtcc gcaccaagaa ggagggtatc ccggacgcgc ggcaggccac caggacgatg 1320

caggcccagc cctacagcac tatgcacaat tccaacaact acctgcactt gtcagtgtct 1380

cgggtggagc tcaagcctgg ggacaacctc aatgtcaact tccacctgcg cacggacgct 1440

ggccaagagg ccaagatccg atactacacc tatctggtta tgaacaaggg gaagttactg 1500

aaggcaggcc gtcaggttcg ggagcctggc caggacctgg tggtcttgtc actgcccatc 1560

actccagaat ttataccttc cttccgcctg gtggcttact acaccctgat tggagctaat 1620

ggccaaaggg aggtggtggc cgactcagtg tgggtggatg tgaaggactc ctgtgtaggc 1680

acgctggtgg tgaaaggtga cccaagagat aaccgacagc ccgcgcctgg gcatcaaacg 1740

acactaagga tcgaggggaa ccagggggcc cgagtggggc tagtggctgt ggacaagggg 1800

gtgtttgtgc tgaacaagaa gaacaaactc acacagagca agatctggga tgtagtagag 1860

aaggcagaca ttggctgcac cccaggcagt gggaagaact atgcgggtgt cttcatggat 1920

gctggcctga ccttcaagac aaaccaaggc ctgcagactg atcagagaga agatcctgag 1980

tgcgccaagc cagctgcccg ccgccgtcgc tcagtgcagt tgatggaaag gaggatggac 2040

aaagctggtc agtacaccga caagggtctg cggaagtgtt gtgaggatgg catgcgtgat 2100

atccctatga agtacagctg ccagcgccgg gctcgcctca tcacccaggg cgagagctgc 2160

ctgaaggcct tcatggactg ctgcaactat atcaccaagc ttcgtgagca gcacagaaga 2220

gaccatgtgc tgggcctggc caggagtgat gtggatgaag acataatccc agaagaagat 2280

attatctcta gaagccactt cccagagagc tggttgtgga ccatagaaga gttgaaagaa 2340

ccagagaaaa atggaatctc tacgaaggtc atgaacatct ttctcaaaga ttccatcacc 2400

acctgggaga ttctggcagt gagcttgtcc gacaagaaag ggatctgtgt ggcagacccc 2460

tatgagatca cagtgatgca ggacttcttc attgacctgc gactgcccta ctctgtggtg 2520

cgcaatgaac aggtggagat cagagctgtg ctcttcaatt accgtgaaca ggagaaactt 2580

aaggtaaggg tggaactgtt gcataaccca gccttctgca gcatggccac tgccaagaag 2640

cggtactacc agaccatcga aatccctccc aagtcctctg tggctgtgcc ttatgtcatt 2700

gtccccttga agatcggcct ccaggaggtg gaggtcaagg ccgccgtctt caaccacttc 2760

atcagtgatg gtgtcaagaa gatactgaag gtcgtgccag aaggaatgag agtcaacaaa 2820

actgtggctg tccgtacact ggatccagaa cacctcggtc aagggggagt gcagagggag 2880

gatgtacctg cagcagacct cagtgaccaa gtgccagaca cagattctga gaccagaatt 2940

ctcctgcaag ggaccccggt ggctcagatg gccgaggacg ctgtggacgg ggagcggctg 3000

aaacacctga tcgtgacccc ctctggctgt ggggagcaga acatgattgg catgacaccc 3060

acggtcattg cagtacacta tctggatcag accgaacagt gggagaaatt cggcctagag 3120

aagaggcaag aagctctgga gctcatcaag aaagggtaca cccagcagct ggctttcaaa 3180

cagcccagct ctgcctatgc tgccttcaac aaccggcctc ccagcacctg gctgacagcc 3240

tatgtggtca aggtcttctc tctggctgcc aacctcatcg ccatcgactc tcaggtcctg 3300

tgtggggctg tcaaatggct gattctggag aaacagaagc cagatggtgt ctttcaggag 3360

gacggaccag tgattcacca agaaatgatt ggtggcttcc ggaacaccaa ggaggcagat 3420

gtgtcgctta cagcctttgt cctcatcgca ctgcaggaag ccagagatat ctgtgagggg 3480

caggtcaaca gccttcccgg gagcatcaac aaggcagggg agtatcttga agccagttac 3540

ctgaacctgc agagaccata cacagtagcc attgctgggt atgccctggc cctgatgaac 3600

aaactggagg aaccttacct caccaagttt ctgaacacag ccaaagatcg gaaccgctgg 3660

gaggagcctg gccagcagct ctacaatgtg gaggccacct cctacgccct cctggccctg 3720

ctgctgctga aagactttga ctctgtgcct cctgtggtgc gctggctcaa cgagcaaaga 3780

tactacggag gtggctatgg ctccacgcag gctaccttca tggtattcca agccttggct 3840

caataccaaa cagatgtccc tgaccacaag gacttgaaca tggatgtgtc cctccacctc 3900

cccagccgca gctccccaac tgtgtttcgc ctgctatggg aaagtggcag tctcctgaga 3960

tcagaagaga ccaagcagaa tgagggcttt tctctgacag ccaaaggaaa aggccaaggc 4020

acactgtcgg tggtgacagt gtatcacgcc aaagtcaaag gcaaagccac ctgcaagaag 4080

tttgacctca gggtcaccat aaaaccagcc cctgagacag ccaagaagcc ccaggatgcc 4140

aagagttcta tgatccttga catctgcacc aggtacttgg gagacgtgga tgctactatg 4200

tccatcctgg acatctccat gatgactggc tttattccag acacaaacga cctggaactg 4260

ctgagctctg gagtagacag atacatttcc aagtatgaga tggacaaagc cttctccaac 4320

aagaacaccc tcatcatcta cctagaaaag atctcacact ccgaagaaga ctgcctgtcc 4380

ttcaaagtcc accagttctt taacgtggga cttatccagc cggggtcggt caaggtctac 4440

tcctactaca atctagagga gtcatgcacc cggttctatc atccggagaa ggacgatgga 4500

atgctgagca agctgtgcca caatgaaatg tgccgctgtg cagaggagaa ctgcttcatg 4560

catcagtcac aggatcaggt cagcctgaat gaacgactag acaaggcttg tgagcctgga 4620

gtggactacg tgtacaagac caagctaacg acgatagagc tgtcggatga ttttgatgag 4680

tacatcatga ccatcgagca ggtcatcaag tcaggctcag atgaggtgca ggcaggtcag 4740

gaacgaaggt tcatcagcca cgtcaagtgc agaaacgccc taaagctgca gaaagggaag 4800

cagtacctca tgtggggcct ctcctccgac ctctggggag aaaagcccaa taccagctac 4860

atcattggga aggacacgtg ggtggagcac tggcccgagg cagaggaatg tcaggatcag 4920

aagaaccaga aacagtgcga agacctcggg gcattcacag aaacaatggt ggttttcggc 4980

tgccccaact ga 4992

<212> Type : DNA

<211> Length : 4992

SequenceName : 44

SequenceDescription :

Sequence

--------

<213> OrganismName : Adra2a

<400> PreSequenceString :

atgttccgcc aggagcagcc gctggccgag ggcagctttg cgcccatggg ctccctgcag 60

ccggatgccg gcaatagcag ctggaacggc accgaggcgc ccggaggcgg cacccgggcc 120

accccttact ccctgcaggt gacgctgacg ctggtgtgcc tggctggcct gctcatgctg 180

ttcaccgtgt ttggcaacgt gctggttatt atcgcagtgt tcaccagccg cgcgctcaaa 240

gcgccccaga acctcttcct ggtgtctctg gcctcagcgg acatcctggt ggccacgctg 300

gtcattccct tttctttggc caacgaggtt atgggctact ggtactttgg taaggtgtgg 360

tgcgagatct acttggccct cgacgtgctc ttttgcacgt cgtccatagt gcacctgtgc 420

gccatcagcc ttgaccgcta ctggtccatc acgcaggcca tcgagtacaa cctgaagcgc 480

acgccgcggc gcatcaaggc catcattgtc actgtgtggg tcatctcggc cgtcatctcc 540

ttcccgccac tcatctccat agagaagaag ggcgctggcg gcgggcagca gccggccgag 600

ccgagctgca agattaacga ccagaagtgg tatgtcatct cgtcgtccat cggctccttc 660

ttcgcgcctt gcctcatcat gatcctggtc tacgtgcgta tctaccagat cgccaagcgt 720

cgcacccgcg tgccgcccag tcgccggggt ccggacgcct gttccgcgcc gccggggggc 780

gccgatcgca ggcccaacgg gctgggcccg gagcgcggcg ctggtaccgc gggcgcggag 840

gccgagccgc tgcccaccca gcttaacggc gccccggggg agcccgcgcc cacccggccg 900

cgcgacgggg atgcgctgga cctagaggag agttcgtcgt ccgagcacgc cgagcggccc 960

caggggcccg gcaaacccga gcgcggtccc cgggccaagg gcaagaccaa ggcgagccag 1020

gtgaaaccgg gggacagtct gccgcggcgc gggcccgggg ctgcggggcc gggggcttcg 1080

gggtccgggc agggagagga gcgtgccggg ggcgccaaag cgtcgcgctg gcgcgggagg 1140

cagaaccgcg agaaacgctt cacgttcgtg ctggcggtgg tgatcggcgt gttcgtggtg 1200

tgttggttcc cgttcttttt cacctacacg ctcatagcgg tcggctgccc ggtgccctac 1260

cagctcttca acttcttctt ctggttcggc tactgcaaca gctcgctgaa ccctgttatc 1320

tacaccattt tcaaccacga cttccgccgc gccttcaaga agatcctctg ccgtggggac 1380

agaaagcgca tcgtgtga 1398

<212> Type : DNA

<211> Length : 1398

SequenceName : 45

SequenceDescription :

Sequence

--------

<213> OrganismName : Nbl1

<400> PreSequenceString :

atgctttggg tcctggtggg gactgttctc cctgtcatgt tgctggctgc cccgccaccc 60

atcaacaagc tggccctgtt tccggacaag agtgcctggt gcgaagccaa gaacatcacg 120

cagattgtgg gccacagcgg ctgtgaggcc aagtctatcc agaacagggc gtgcctagga 180

caatgcttca gttacagcgt ccccaacacc ttcccgcagt ccacagagtc cctggtgcac 240

tgtgactcct gcatgccggc ccagtccatg tgggagattg tgaccttgga gtgccccggc 300

cacgaggagg tgcccagggt ggacaagctg gttgagaaga ttgtacactg cagctgccaa 360

gcctgcggca aggaacccag tcatgagggc ctgaacgtct acatgcaggg tgaagatggg 420

ccaggctccc agccaggatc gcactcccac tcccatcccc accctggtgg ccagacccct 480

gaacctgaag aacctcctgg agcccctcaa gttgaggaag agggggctga ggactga 537

<212> Type : DNA

<211> Length : 537

SequenceName : 46

SequenceDescription :

Sequence

--------

<213> OrganismName : Ache

<400> PreSequenceString :

atgaggcctc cctggtaccc cctgcataca ccctccctgg cttctccact cctcttcctc 60

ctcctctccc tcctgggagg aggggcaagg gctgagggcc gggaagaccc tcagctgctg 120

gtgagggttc gagggggcca gctgaggggc atccgcctga aggcccctgg aggcccagtc 180

tcagcttttc tgggcatccc ctttgcagag ccacctgtgg gctcacgtag atttatgcca 240

ccagagccca agcggccctg gtcaggaata ttggatgcta ccaccttcca aaatgtctgc 300

taccaatacg tggacaccct gtaccctggg tttgagggta ccgagatgtg gaaccccaat 360

cgagagctga gtgaagactg cctttatctt aatgtgtgga caccataccc caggcctact 420

tctcccacac ctgtcctcat ctggatctat gggggtggtt tctacagtgg agcatcctcc 480

ttggacgtgt atgacggccg tttcctggcc caggttgagg gaaccgtgtt ggtatctatg 540

aactaccgag tgggaacctt tggcttcttg gctctaccag gaagcagaga cgcccctggc 600

aatgtaggcc tgctggatca acggcttgcc ttgcaatggg tacaagaaaa tatcgcagcc 660

tttgggggag acccaatgtc agtgactctg tttggggaga gtgcaggtgc agcctcagtg 720

ggcatgcaca ttctgtccct gcccagcagg agcctcttcc acagggctgt cctgcagagt 780

ggcacaccca acgggccctg ggccactgtg agtgcgggag aggccaggcg cagggccaca 840

ctgctggccc gccttgtggg ctgtccccca ggtggcgctg gtggcaatga caccgagctg 900

atatcctgct tgaggacaag gcccgctcag gacctggtgg accacgagtg gcatgtgctg 960

cctcaagaaa gtatcttccg gttttccttc gtgcctgtgg tggacgggga tttcctcagt 1020

gacacgccgg aggccctcat caatactgga gattttcaag acctgcaggt gctggtgggt 1080

gtggtgaagg acgagggctc ctactttctg gtttacgggg tcccaggctt cagcaaagac 1140

aatgaatctc tcatcagccg ggcccagttc ctggctgggg tgcggatcgg tgtaccccaa 1200

gcgagtgacc tggcggccga ggctgtggtc ctgcattata cagactggct gcaccctgag 1260

gaccctgccc acctgagaga tgccatgagt gcggtggtag gcgaccacaa cgttgtgtgc 1320

cctgtggccc agctggctgg gcgactggct gcccaagggg ctcgggtcta tgcctacatc 1380

tttgaacacc gtgcctccac attgacttgg cccctctgga tgggggtgcc ccatggctat 1440

gaaatcgagt tcatctttgg gctccccctg gatccctcac tgaactacac cgtggaggag 1500

agaatctttg ctcagcgact tatgaaatac tggaccaatt ttgcccgcac aggggtgccc 1560

atggatggtt gtgaaaaaag gaagaaatac ccacaaagga aaggggatgg agagaaagcc 1620

acagacaaga gacaaaaact gctagagacc tttaatccca gtacaccaga gacagaggac 1680

attctgatgc ggggtgccct ggccgtagac acgctggacg aggcggagcg ccagtggaag 1740

gccgagttcc accgctggag ctcctacatg gtgcactgga agaaccagtt cgaccactat 1800

agcaagcagg aacgctgctc agacctgtga 1830

<212> Type : DNA

<211> Length : 1830

SequenceName : 47

SequenceDescription :

Sequence

--------

<213> OrganismName : c1qb

<400> PreSequenceString :

atgaagacac agtggagtga gatcttgaca cccctgttgc tgctgctcct gggtttgctc 60

catgtctcct gggcccaaag cagctgtact gggtcccctg gcatccctgg ggtccctggc 120

atccctgggg tccctggctc tgatggcaaa ccaggcactc cagggataaa aggagagaaa 180

gggctccccg gactggctgg agaccatggt gagttaggag agaaagggga tgcagggatc 240

cctgggatcc caggcaaagt tggccccaag ggtcccgtcg gccctaaggg tgctccaggc 300

ccccctggac cccgcggtcc caaaggtgac tctggagact acaaggctac ccagaaagta 360

gccttctctg ccctgaggac ggtcaacagc gccctgcgac caaaccaggc cattcgcttt 420

gaaaaggtga tcaccaatgt taatgataac tacgagccgc gcagtggcaa gttcacctgc 480

aaggtacctg gcctctacta cttcacctac cacgccagtt cccgcgggaa tctgtgtgtg 540

aacatcgtgc gcggccgcga ccgagaccgc atgcagaaag ttctcacctt ctgcgactat 600

gcccaaaaca ccttccaggt caccacgggt ggggtagtct tgaagctgga gcaggaagag 660

gttgttcacc tgcaggccac agacaagaac tccctgctgg gcgtcgaggg agccaatagc 720

atcttcactg gctttctgct tttccctgac atggatgtat ga 762

<212> Type : DNA

<211> Length : 762

SequenceName : 48

SequenceDescription :

Sequence

--------

<213> OrganismName : c1qc

<400> PreSequenceString :

atggttgtag gaaccagctg ccagccccag catggactct acctgctgct gctccttctg 60

gccctacccc tcaggagcca ggccaacgct ggctgctatg ggatcccagg gatgccaggc 120

ctgccgggaa cccctgggaa ggatgggcat gatggacttc aggggcccaa gggtgagccg 180

ggaatcccag ccatccctgg gacacaagga cccaagggtc agaagggcga gccgggtatg 240

cctggccatc gtgggaaaaa cggccccatg gggacctctg ggtcgccagg ggatccaggc 300

cccaggggtc ctcccgggga gccgggtgag gagggtcgat acaaacagaa gcatcagtcg 360

gtgttcacgg tcacccggca gaccgcgcag tacccagcgg ccaatggcct tgtcaagttc 420

aattccgcca tcaccaatcc tcagggggat tacaacacaa acacggggaa gttcacctgc 480

aaagtgcccg gcctctacta cttcgtccac cacacatccc agacggccaa cctgtgcgtg 540

cagctgctcc tcaacaatgc caaggtgacc agcttctgcg accacatgtc caacagcaag 600

caggtcagct caggaggagt actcctgcgg ctgcagaggg gcgacgaggt gtggctggcc 660

gtcaacgact acaacggcat ggtgggcact gagggctctg acagcgtctt ctctggtttc 720

ctactgtttc ctgactag 738

<212> Type : DNA

<211> Length : 738

SequenceName : 49

SequenceDescription :

Sequence

--------

<213> OrganismName : Timp1

<400> PreSequenceString :

atggcgccct ttgcatctct ggcctctggc atcctcttgt tgctatcatt gatagcttcc 60

agtaaagcct gtagctgtgc cccaacccac ccacagacag ctttctgcaa ctcggacctg 120

gttataaggg ctaaattcat gggttcccca gaaatcatcg agaccacctt ataccagcgt 180

tatgagatca agatgactaa gatgctcaaa ggattcgacg ctgtgggaaa tgccacaggt 240

ttccggttcg cctacacccc agccatggag agcctctgtg gatatgtcca caagtcccag 300

aaccgcagcg aggagtttct catcgcgggc cgtttaagga acggaaattt gcacatcact 360

gcctgcagct tcctggttcc ctggcataat ctgagccctg ctcagcaaaa ggccttcgta 420

aagacctata gtgctggctg tggggtgtgc acagtgtttc cctgttcagc catcccttgc 480

aaactggaga gtgacagtca ttgcttgtgg acagatcaga tcctcatggg ctctgagaag 540

ggctaccaga gcgatcactt tgcctgcctg ccacggaatc cagatttgtg cacctggcaa 600

taccttgggg tctcgatgac ccgaagcctt cccctggcaa aagctgaagc ctga 654

<212> Type : DNA

<211> Length : 654

SequenceName : 50

SequenceDescription :

Sequence

--------

<213> OrganismName : Lum

<400> PreSequenceString :

atgaatgtat gtacgttcac tcttgtcttg gcattagtcg gtagtgtcag tggccaatac 60

tatgattatg atgcccctct cttcatgtat ggggaactgt cacccaactg tgcaccagaa 120

tgtaactgtc cccacagcta cccaactgcc atgtactgtg atgatctcaa gttgaaaagt 180

gtgcccatgg tgccccctgg catcaagtac ctttacctga ggaataacca gatcgaccat 240

attgatgaaa aggcctttga gaatgtaacg gatctgcagt ggctcattct tgaccacaat 300

cttctagaaa actccaagat caaaggaaag gtcttctcta agctgaaaca actgaagaag 360

ctgcatataa actacaacaa tttgaccgag tccgtgggtc cgctcccaaa gtccctacaa 420

gacctgcagc tggccaataa taagatcagc aagctgggct ccttcgacgg gctggtaaac 480

ctgaccttca tttaccttca acacaaccag ctcaaagagg aggctgtctc ggcttctctg 540

aaaggcctta agtcactcga atacctcgac ttgagcttca atcagatgag caagctgccc 600

gctggccttc caacatctct tctaactctc tacctagaca acaataagat caccaacatt 660

cctgatgagt atttcaatcg cttcaccggg cttcaatact tgcgtttatc tcacaatgag 720

ctggctgaca gtggcgtgcc tggaaactca tttaacatat catctctgct cgagctcgat 780

ctctcctaca ataagctcaa gagtatacca accgttaatg aaaaccttga aaactattac 840

ctggaggtca ataaactcga aaagtttgat gtcaagagct tctgtaagat cctgggacca 900

ctatcttact ccaagatcaa gcatttgcgc ttggatggca atcccctcac tcaaagcagt 960

ctgccccctg acatgtacga gtgtctacgt gtagcaaatg aaatcacggt taattaa 1017

<212> Type : DNA

<211> Length : 1017

SequenceName : 51

SequenceDescription :

Sequence

--------

<213> OrganismName : Csrp3

<400> PreSequenceString :

atgccgaact ggggtggagg tgcaaaatgt ggagcctgcg acaagacggt ttaccatgca 60

gaagaaatcc agtgcaatgg gaggagcttc cataagacct gtttccactg catggcctgc 120

aggaaggctc tggacagcac cacagtggca gctcatgagt cagagatcta ctgtaaggtc 180

tgctatgggc gcaagtatgg ccccaagggg atcgggtttg gacaaggcgc tggctgcctc 240

agcacagaca ccggcgagca ccttggcctg cagttccaac aatccccaaa gccagcacgt 300

gcagccacca cgagcaaccc ttccaagttc tctgcgaagt ttggagaatc agagaagtgc 360

ccacgatgcg gaaagtcggt atatgctgct gagaaggtca tgggaggtgg caagccctgg 420

cataagacct gctttcgctg cgccatctgt gggaagagcc tggagtccac aaatgtcact 480

gacaaggatg gggagcttta ttgcaaagtt tgctatgcca aaaattttgg ccccacaggc 540

attgggtttg gagggcttac acaccaagtg gaaaagaagg agtga 585

<212> Type : DNA

<211> Length : 585

SequenceName : 52

SequenceDescription :

Sequence

--------

<213> OrganismName : Stmn4

<400> PreSequenceString :

atgaccctcg cagcctataa ggagaagatg aaggaactcc cactagtgtc tctgttctgc 60

tcctgttttc tgtctgatcc cctgaataaa tcatcctaca aatatgaagg ctggtgtggg 120

agacagtgta ggaggaaagg tcaaagccag cggaaaggca gtgctgactg gagagaaaga 180

agagaacagg cagatacggt agacctgaac tggtgtgtca tctctgatat ggaagtcatc 240

gagctgaata agtgtacctc gggccagtcc tttgaagtca tcctgaagcc accttccttt 300

gacggggtgc ctgagtttaa tgcctccctc ccaagacgtc gagacccatc gctagaagag 360

atacagaaga agctagaagc agcagaggag cgaaggaagt accaggaagc tgagctccta 420

aaacaccttg cagagaaacg agagcatgag cgtgaggtaa tccagaaagc tatcgaggaa 480

aacaacaact tcatcaagat ggcgaaagag aagctggccc agaagatgga gtccaataag 540

gaaaaccggg aggcccatct ggctgccatg ttggagcggc tgcaagagaa ggacaagcac 600

gcagaggagg tgcggaaaaa caaggagctg aaggaagagg cctccaggta a 651

<212> Type : DNA

<211> Length : 651

SequenceName : 53

SequenceDescription :

Sequence

--------

<213> OrganismName : Uchl1

<400> PreSequenceString :

atgcagctga aaccgatgga gattaacccc gagatgctga acaaagtgtt ggccaagctg 60

ggggtcgccg ggcagtggcg ctttgccgac gtgctagggc tggaggagga gactctgggc 120

tcagtgccat ctcctgcctg cgccctgctg ctgctgtttc ccctcacggc ccagcatgaa 180

aacttcagga aaaaacaaat tgaggaactg aagggacaag aagttagccc taaagtttac 240

ttcatgaagc agaccatcgg gaactcctgt ggtaccattg ggctgatcca cgcagtggcc 300

aataaccaag acaagctgga atttgaggat ggatcagtcc tgaaacagtt tctgtctgaa 360

acggagaagt tgtcccctga agacagagcc aagtgtttcg agaagaacga ggccattcag 420

gcagcccatg actccgtggc ccaggagggc cagtgccggg tagacgacaa agtgaatttc 480

cattttatcc tgttcaataa tgtggacggc cacctctacg agctcgatgg gcgaatgcct 540

ttcccggtga accatggcgc cagttcagag gactctctgc tgcaggatgc cgccaaggtc 600

tgcagagaat tcactgagcg cgagcaggga gaggtccgct tctccgcagt ggctctctgc 660

aaagcagcct aa 672

<212> Type : DNA

<211> Length : 672

SequenceName : 54

SequenceDescription :

Sequence

--------

<213> OrganismName : Ctsk

<400> PreSequenceString :

atgtgggtgt tcaagttttt gctgctaccc gtggtgagct ttgctctatc cccggaggaa 60

acgctggaca cgcagtggga gctgtggaag aagacccacg ggaagcagta caacagcaag 120

gtggatgaaa tctctcggcg tttaatttgg gaaaaaaacc tgaagaaaat ttctgtccat 180

aatcttgagg cctctcttgg tgcccatacg tatgagctgg ccatgaatca cctgggagac 240

atgaccagcg aagaagtggt tcagaagatg actggactca gagtgccacc ttcgcgttcc 300

ttcagtaatg acactctcta taccccagag tgggaaggca gagtcccaga ctccatcgac 360

tatcgaaaga aaggctatgt tactccagtc aaaaaccagg gccagtgtgg ttcctgttgg 420

gctttcagct ctgcgggtgc cctggagggc caactcaaga agaaaactgg caaactctta 480

gctctgagtc cccagaatct tgtggactgt gtgtctgaga actatggctg tggaggcggc 540

tatatgacca ctgccttcca atatgtgcag cagaatggag gcattgactc tgaagacgct 600

tacccgtatg tggggcagga tgaaagttgt atgtataacg ccacggcaaa ggcagctaag 660

tgcagagggt acagagagat ccctgtgggg aacgagaaag ccctgaagag agcagtggct 720

cgggtaggac ccgtctctgt gtccatcgat gcaagcttga catctttcca attttacagc 780

agaggtgtgt actatgacga aaactgcgac cgtgataatg tgaaccatgc cgtgttggtg 840

gtgggctatg gcacccagaa gggaaataag tactggataa ttaaaaacag ctggggagaa 900

agctggggaa acaaaggcta tgttctcttg gctcggaata agaacaatgc ctgtggcatt 960

accaacctgg ccagcttccc caagatgtga 990

<212> Type : DNA

<211> Length : 990

SequenceName : 55

SequenceDescription :

Sequence

--------

<213> OrganismName : Lxn

<400> PreSequenceString :

atgccgccca gtcgaccccc gccgccggcg ctgctactga gcctctggct actgccgagc 60

ctcgcactgg ccgctgctgc cgcagcgccg gtgaagccgg agtacaccgt cgtctccggg 120

cagcccccgg ccgtggggct gccacgcggg atcctgcagc tggcggcgcg cgctgcgctg 180

cacttcctca acttccgcgc cggctcgccc agcgcgctac gggtgctggc cgccgtgcag 240

gagggccgcg cgtgggttga ccctcaggag ggctgcgagg tggacctggt gtttagcaca 300

gagcactaca accccgagca ggaaggagag gaacgtttgg gcaaatgttc tgcgagggtg 360

tttttcaaga atgaaaagcc cagaccggcc gttaacgtaa cctgcacacg gctctttgag 420

aaatcgaaga gacaagaaaa ggattatctg ctttacaagc agatgaagca actgaagacc 480

cgtctggacg tcgtcagcat acctgataat caaggtcaca ttgacccttc actgagaccc 540

ctgtgggact tggctttcct cggcagctcg tacgtgatgt gggagaagac aagccagttt 600

ctgcactact acctgatcca gctcagcagc gtgaagcagt tgaaaacaga tgatgacagc 660

attgattttg acttcactgt tctacttcat gaattctcaa cacaggaaat cattccctgc 720

cgtattcata tggtctggta ccctggcaag cccctaaaag tcaattacca ttgtcaagag 780

ctacagaacc cagaagaaac ctctggaact gcggaagcat cagctatgga gccagccgag 840

ttcagtactt tctaa 855

<212> Type : DNA

<211> Length : 855

SequenceName : 56

SequenceDescription :

Sequence

--------

<213> OrganismName : Nsf

<400> PreSequenceString :

gcaggagaga ctatgcaagc tgcgagatgc cctactgatg aattgtctct aagcaactgt 60

gcagttgtga acgaaaagga ttaccagtct ggccagcatg tgatggtgag gacgtccccc 120

aaccacaagt acatatttac gctgcggacc catccgtccg tggttcccgg atgcatcgcg 180

ttcagcttac cccagcgaaa atgggctgga ctttctattg ggcaggacat agaagtcgcc 240

ttgtattcgt ttgacaaagc caagcagtgc atcggcacaa tgaccatcga gatcgacttc 300

ctgcagaaaa agaacatcga ctccaaccct tacgacaccg acaagatggc tgccgagttc 360

atccagcagt tcaacaacca ggccttctca gtgggacagc agcttgtctt tagcttcaat 420

gataagctct ttggattgct ggtgaaggac atcgaagcca tggatcccag catcctgaag 480

ggagagcctg cgtcaggtaa aaggcagaag atggaaatag gaattcaagt gtttcacagt 540

caagttgcat ttgaaaaagc agaaaactca tcactcaatc ttattggcaa ggctaaaacc 600

aaggaaaatc gccagtctat catcaatccc gactggaact ttgagaaaat ggggatcggc 660

ggtctggata aggagttttc agatattttc cgacgagcat ttgcttctcg ggtgttccct 720

ccggagatcg tggaacaaat gggttgcaaa catgttaaag gcatcctgtt atacggaccc 780

cctggttgtg gtaagactct cttggctcga cagattggca agatgctgaa tgcaagagag 840

cccaaggtgg tcaatgggcc ggaaatcctt aacaaatatg tgggagaatc agaggccaac 900

atccgcaagc tctttgctga tgcagaagag gagcagagac ggctcggtgc caacagcggc 960

ctgcacatca tcatctttga tgaaatcgac gccatctgca agcagagagg gagcatggcc 1020

ggcagcaccg gggtgcacga cactgtggtc aaccagctgc tgtccaagat cgatggcgtc 1080

gagcagctga acaacatcct cgttattgga atgaccaatc gaccagattt gattgatgag 1140

gctctccttc gacctggaag actggaagtt aagatggaga taggcttacc agatgagaag 1200

ggccgactgc agatccttca catccacaca gcaaggatgc gagggcacca gctactgtct 1260

gcggatgtgg acatcaagga actggctgtg gagactaaga atttcagtgg cgccgagctg 1320

gagggcctgg tgcgagcagc gcagtccaca gccatgaaca gacacataaa ggccagcaca 1380

aaagtggaag tagacatgga gaaggcagag agcctgcagg tgacaagagg ggacttcctc 1440

gcttccctgg agaatgatat caaaccggca ttcggcacaa accaagagga ctatgcaagt 1500

tacattatga atggaatcat caagtggggt gaccccgtga cccgagtcct cgacgacgga 1560

gagctgctgg tccagcaaac taaaaacagt gaccgcacac cgctggttag cgtccttctg 1620

gaaggccctc ctcacagcgg gaagactgcc ctcgctgcaa agattgccga ggaatcaaac 1680

ttccccttca tcaagatctg ctcccccgat aagatgattg ggttctctga gacagccaag 1740

tgtcaggcca tgaagaagat ctttgatgat gcttacaaat ctcagctcag ttgtgtggtc 1800

gtggatgaca ttgagaggtt gctcgattac gtccccatcg gcccccggtt ctccaacctg 1860

gtgttacaag ctctgctcgt gttactgaaa aaggcacctc ctcagggccg caagcttctc 1920

atcatcggga ccaccagccg caaagatgtc ctgcaggaaa tggagatgct caacgccttc 1980

agcaccacca tccacgtgcc caacatcgcc acaggagagc agctgctgga agctctggag 2040

cttttgggca acttcaaaga taaggaacgc accacaattg ctcagcaagt caaagggaag 2100

aaagtctgga taggaatcaa gaagttacta atgttgattg agatgtccct gcagatggac 2160

cctgagtacc gtgtgaggaa attcttggcc ctcatgagag aagaaggcgc tagtcccctg 2220

gactttgact ga 2232

<212> Type : DNA

<211> Length : 2232

SequenceName : 57

SequenceDescription :

Sequence

--------

<213> OrganismName : Adora1

<400> PreSequenceString :

atgccgccct acatctcggc cttccaggct gcctacattg gcatcgaggt gctcattgcc 60

ttggtctctg tgcccggaaa tgtactggtg atttgggctg tgaaggtgaa ccaggcactt 120

cgcgatgcca ccttctgctt catcgtgtca ctggcggtag ctgatgtggc cgttggcgcc 180

ctggtcatcc cactggccat ccttatcaac attgggccac agacctactt ccacacctgc 240

ctcatggtgg cctgccctgt cctcatcctc acccagagct ccattctggc tctgctcgcc 300

attgctgtgg atcgatacct ccgagtcaag atccctctcc ggtacaagac agtggtgacc 360

cagcggcggg cggcagtggc catagctggc tgctggattc tctcccttgt ggtaggcctg 420

acacccatgt ttggctggaa caacctgagt gtggtagagc aagactggag agccaacggc 480

agtgttgggg agcccgtgat caagtgtgag tttgagaagg ttatcagcat ggagtacatg 540

gtctacttca acttcttcgt ctgggtgctg ccgccactgc tcctcatggt cctcatctac 600

ctggaggtct tctacctgat ccgtaagcag ctcaacaaaa aggtgtcagc ctcctccggt 660

gacccccaga agtactacgg gaaggagctg aagatcgcca agtcgctggc cctcatcctc 720

ttcctctttg ccctcagctg gctgccgctg catatcttga actgtatcac cctcttctgc 780

cccacctgcc agaaacccag cattctgatc tacatcgcca tcttcctcac acacggcaac 840

tccgccatga accccatcgt ctatgccttc cggatccaca agttccgggt cacctttctg 900

aagatttgga atgaccactt ccgatgccag cctaagcctc ccatcgatga agacctccca 960

gaggagaaag ctgaggacta g 981

<212> Type : DNA

<211> Length : 981

SequenceName : 58

SequenceDescription :

Sequence

--------

<213> OrganismName : Adora2a

<400> PreSequenceString :

atgggctcct cggtgtacat cacggtggag ctggccatcg ctgtgctggc catcctgggc 60

aacgtgctcg tgtgctgggc cgtgtggatc aacagtaacc tgcagaacgt caccaacttc 120

tttgtggtat cgctggcggc ggctgacatt gcagtgggtg tgctcgccat ccccttcgct 180

atcaccatca gcaccggctt ctgcgccgcc tgccacggct gcctcttctt cgcctgtttt 240

gtcctggtcc tcacgcagag ttccatcttt agcctcttgg ctatcgccat cgaccgctac 300

atcgccatcc gaattccact ccggtacaat ggcttggtga caggtgtgag ggcgaagggc 360

atcattgcaa tttgctgggt gctgtcgttt gccattggcc tgacccccat gctgggctgg 420

aacaactgca gtcagaaaga cgggaactcc acgaagacct gcggcgaggg ccgggtgacc 480

tgtctgttcg aggacgtggt gcccatgaat tacatggttt actacaactt ctttgcgttc 540

gtgttactgc cccttctgct catgctggcc atctacctac ggatttttct ggcggcccgg 600

agacagctga agcagatgga gagccagccc ctgccagggg agcggactcg gtccacgctg 660

cagaaggagg tccacgctgc caagtccctg gccatcatcg tcgggctctt tgctctgtgc 720

tggttgccgc tgcacatcat caactgtttc accttcttct gctccacgtg ccggcacgcc 780

cctccgtggc tcatgtacct ggccatcatc ctctcccaca gcaactccgt cgtcaacccc 840

ttcatctacg cctacaggat ccgggagttc cgccagacct tccggaagat catccgaacc 900

cacgtcctga ggcggcagga acccttccag gcagggggtt ccagtgcctg ggccctggca 960

gctcacagca ctgagggaga gcaggttagc ctccgcctta atggccaccc cctgggggta 1020

tgggccaacg gcagtgccac ccattccgga cggcggccca atggctacac tctggggctg 1080

gggggtggag ggagtgccca aggctctcct cgggatgtgg agcttcctac ccaggagcgc 1140

caggaaggcc aagagcaccc tggcctaagg ggtcatctgg tccaggctag agtaggagct 1200

tcctcatggt cttcagagtt tgccccttcc tga 1233

<212> Type : DNA

<211> Length : 1233

SequenceName : 59

SequenceDescription :

Claims (7)

1. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the manufacture of a formulation for reducing joint swelling in the treatment of gout, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are of formula II or formula III:

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH.
2. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the manufacture of a formulation for reducing the expression level of inflammasome NLRP3 in the treatment of gout, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are of formula II or formula III:

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH.
3. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the preparation of a formulation for the treatment of gout pain by up-regulating the mRNA expression of the adenosine a1 receptor, the adenosine A2A receptor, up-regulating the adenosine a1 receptor protein expression, and reducing the adenosine A2A receptor protein expression, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are represented by formula II or formula III:

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH.
4. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the preparation of an autophagy-promoting level formulation in the treatment of gout, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are of formula II or formula III:

   

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH.
5. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the preparation of a formulation for promoting degradation of p62 in the treatment of gout, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are of formula II or formula III:

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH.
6. Use of N6- (2-hydroxyethyl) adenosine and derivatives thereof in the preparation of formulations of regulatory gene sequences SEQ ID No.48 to SEQ ID No.59 in the treatment of gout, wherein the derivatives of N6- (2-hydroxyethyl) adenosine are represented by formula II or formula III:

   

   wherein R1 is C (CH3)2CH2OH, CH2CH2CH2OH, CH (CH3) CH2OH, and R2 is H; r is CH3O, H, OH;

   the N6- (2-hydroxyethyl) adenosine and the derivatives thereof can regulate the down-regulation expression of mRNA from SEQ ID No.48 to SEQ ID No.56 and regulate the up-regulation expression of mRNA from SEQ ID No.57 to SEQ ID No. 59.
7. 7. The use according to any one of claims 1 to 6, wherein the N6- (2-hydroxyethyl) adenosine is isolated directly or indirectly from Cordyceps cicadae, Cordyceps militaris, Cordyceps sinensis and Cordyceps sinensis mycelia, fruiting bodies and spores containing N6- (2-hydroxyethyl) adenosine, or is artificially synthesized.

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