CN108318465B - Method for detecting concentration of heme solution - Google Patents
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- CN108318465B CN108318465B CN201810129543.1A CN201810129543A CN108318465B CN 108318465 B CN108318465 B CN 108318465B CN 201810129543 A CN201810129543 A CN 201810129543A CN 108318465 B CN108318465 B CN 108318465B
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Abstract
The invention provides a method for detecting the concentration of a heme solution, and belongs to the field of biological detection. The method uses thiamine to replace luminol, is low in cost and environment-friendly, enables a fluorescent signal to be stable, improves reproducibility of a detection result, decomposes artemisinin in the presence of heme to generate superoxide radical, generates thiamine without fluorescence, oxidizes and generates fluorescent sulfur pigment in the presence of superoxide radical, detects the concentration of heme by using a fluorescence spectrometry, and is better in reproducibility compared with a chemiluminescence method. The data of the examples show that the detection method provided by the invention performs 6 repeated measurements on 300nmol/L of heme, and the relative standard deviation is 6.8%.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a method for detecting the concentration of a heme solution.
Background
Heme is a natural porphyrin iron compound, plays a key role in oxygen transport, and enables the normal operation of human organs. Excessive heme may lead to permanent secondary damage to the brain following hemorrhagic stroke, and a deficiency in heme may induce neurosphere expression in nerve cells, accelerating endogenous carbon monoxide production. In addition, it is widely used in the fields of medicine, food and others as a novel iron supplement.
At present, methods for detecting heme are reported to be a chemiluminescence method, an electrochemical method, a capillary electrophoresis method, a fluorescence spectroscopy method and the like, and the problem of poor reproducibility of the detection method exists, for example, xu et al use an Artemisinin-luminol system to realize chemiluminescence detection of heme, heme with different concentrations is added into a buffer solution containing luminol and Artemisinin to perform chemiluminescence detection (see "Artemisinin-luminol chemiluminescence for sensitive haemostasis detection a luminescent phosphor as a detector", g.b.xu et al, anal.chem.,2017, 89: 6160-6165), but the luminol luminescence time is limited, and the sample needs to be measured immediately after being prepared, and the reproducibility is poor.
Disclosure of Invention
The invention aims to provide a method for detecting the concentration of a heme solution, which has good reproducibility.
The invention provides a method for detecting the concentration of a heme solution, which comprises the following steps:
(1) mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, incubating the obtained mixed solution to be detected to obtain an incubation solution to be detected, and detecting the fluorescence intensity of the incubation solution to be detected;
(2) obtaining the concentration of heme in the heme solution to be detected according to the fluorescence intensity of the incubation solution to be detected obtained in the step (1) and a preset standard curve; the standard curve takes the concentration of the heme standard solution as an abscissa and the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as an ordinate.
Preferably, the incubation temperature in the step (1) is 15-40 ℃, and the incubation time is 5-30 min.
Preferably, the molar ratio of artemisinin to thiamine in the heme solution to be detected in the step (1) is 10-2000: 0.5-500.
Preferably, the inorganic base in step (1) is sodium hydroxide or potassium hydroxide.
Preferably, in the step (1), the inorganic base is added in the form of an inorganic base solution, and the concentration of the inorganic base solution is 5-500 mmol/L.
Preferably, the artemisinin in the step (1) is added in the form of an artemisinin solution, and the concentration of the artemisinin solution is 0.05-50 mmol/L.
Preferably, the thiamine in the step (1) is added in the form of a thiamine solution, and the concentration of the thiamine solution is 5 mu mol/L-2 mmol/L.
Preferably, the linear range of the standard curve in the step (2) is 2.0-300.0 nmol/L.
The invention provides a method for detecting the concentration of a heme solution, which comprises the following steps: mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, incubating the obtained mixed solution to be detected to obtain an incubation solution to be detected, and detecting the fluorescence intensity of the incubation solution to be detected; obtaining the concentration of the heme in the heme solution to be detected according to the fluorescence intensity of the incubation solution to be detected and a preset standard curve; the standard curve takes the concentration of the heme standard solution as an abscissa and the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as an ordinate. The method uses thiamine to replace luminol, is low in cost and environment-friendly, enables a fluorescent signal to be stable, improves reproducibility of a detection result, decomposes artemisinin in the presence of heme to generate superoxide radical, generates thiamine without fluorescence, oxidizes and generates fluorescent sulfur pigment in the presence of superoxide radical, detects the concentration of heme by using a fluorescence spectrometry, and is better in reproducibility compared with a chemiluminescence method. The data of the examples show that the detection method provided by the present invention performs 6 replicates on 300nmol/L hemoglobin solution with a relative standard deviation of 6.8%. And has good selectivity.
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FIG. 1 is a graph showing the change in fluorescence intensity after adding different concentrations of hemoglobin standard solutions in example 1 of the present invention;
FIG. 2 is a plot of the fluorescence intensity versus hemoglobin concentration for the system of example 1, with the inset showing the fluorescence intensity variation and the standard equation for hemoglobin;
FIG. 3 shows the change of fluorescence intensity of the system after the addition of other interfering substances in example 2 of the present invention.
Detailed Description
The invention provides a method for detecting the concentration of a heme solution, which comprises the following steps:
(1) mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, incubating the obtained mixed solution to be detected to obtain an incubation solution to be detected, and detecting the fluorescence intensity of the incubation solution to be detected;
(2) obtaining the concentration of heme in the heme solution to be detected according to the fluorescence intensity of the incubation solution to be detected obtained in the step (1) and a preset standard curve; the standard curve takes the concentration of the heme standard solution as an abscissa and the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as an ordinate.
The method comprises the steps of mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water to obtain a mixed solution to be detected, incubating to obtain an incubation solution to be detected, and detecting the fluorescence intensity of the incubation solution to be detected. In the invention, the incubation temperature is preferably 15-40 ℃, more preferably 25 ℃, and the incubation time is preferably 5-30 min, more preferably 20 min.
In the invention, the molar ratio of artemisinin to thiamine in the heme solution to be detected is preferably 10-2000: 0.5-500.
In the invention, the detection principle of the concentration of the heme solution is as follows: decomposing artemisinin in the presence of heme to produce superoxide radical; thiamine itself is not fluorescent and in the presence of superoxide radicals, oxidizes to produce a fluorescent thioflavine, as shown in the following formula:
in the invention, the pH value of the mixed solution to be detected is preferably 12-13. The pH value of the mixed solution to be measured is adjusted by the use amount of the inorganic base. In the present invention, the inorganic base is preferably sodium hydroxide or potassium hydroxide. In the invention, the inorganic base is preferably added in the form of an inorganic base solution, and the concentration of the inorganic base solution is preferably 5-500 mmol/L, more preferably 5, 10, 20, 50, 100, 200, 500 mmol/L; the volume of the inorganic base solution was 870. mu.L.
In the invention, the concentration of the heme solution to be detected is preferably 2.0-300.0 nmol/L.
The metal ions, glucose and amino acid have no influence on the detection method of the concentration of the heme solution provided by the invention, and the selectivity of the detection method of the invention is not influenced. In the present invention, the metal ions preferably include cobalt ions, magnesium ions, copper ions, zinc ions, and iron ions; the amino acids preferably include phenylalanine, leucine, glycine, alanine and cysteine.
In the invention, the artemisinin is preferably added in the form of an artemisinin solution, and the preferable concentration of the artemisinin solution is 0.05-50 mmol/L, and more preferably 5 mmol/L; the artemisinin solution is preferably added in a volume of 10 μ L.
In the invention, the thiamine is preferably added in the form of a thiamine solution, and the concentration of the thiamine solution is preferably 5 mu mol/L-2 mmol/L, and more preferably 1 mmol/L; the addition volume of the thiamine solution is preferably 100. mu.L.
The invention has no special limit on the dosage of the Ethylene Diamine Tetraacetic Acid (EDTA) and has no influence on the mixed solution to be detected.
The adding sequence of the heme solution to be detected, the disodium ethylene diamine tetraacetate, the artemisinin, the thiamine, the inorganic base and the water is not specially limited, the adding sequence known by the technical personnel in the field is adopted, and specifically, the disodium ethylene diamine tetraacetate, the artemisinin solution, the thiamine solution and the heme solution to be detected are sequentially added into the inorganic base solution.
The method for detecting the fluorescence intensity of the incubation solution to be detected is not particularly limited, and the method for detecting the heme by using fluorescence spectrometry, which is well known to those skilled in the art, can be used.
After the fluorescence intensity value of the incubation solution to be detected is obtained, the concentration of the heme in the heme solution to be detected is obtained according to the obtained fluorescence intensity of the incubation solution to be detected and a preset standard curve; the standard curve takes the concentration (c) of the heme standard solution as an abscissa and the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as an ordinate. In the embodiment of the invention, the standard curve is Δ F ═ 32.01+23.54c (c unit is nmol/L), the linearity coefficient is 0.9954, the linearity range is 2.0-300.0 nmol/L, and the detection limit is 0.68 nmol/L. In the present invention, the method for acquiring the standard curve preferably includes the following steps:
providing a heme standard solution;
mixing disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, adding the heme standard solution, and detecting the fluorescence intensity after the heme standard solution is added;
and taking the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as the ordinate, taking the concentration of the heme standard solution as the abscissa, and performing linear fitting to obtain a standard curve.
The invention mixes standard heme with water to obtain a heme standard solution, and measures the fluorescence intensity of the heme standard solution. In the present invention, the concentration of the heme standard solution is preferably 0.002,0.005,0.01,0.05,0.1,0.2,0.3,0.5,0.8,2.0,5.0, or 10.0. mu. mol/L. The source of the standard heme is not particularly limited in the present invention, and a heme standard known to those skilled in the art may be used.
After obtaining the heme standard solution, mixing disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, adding the heme standard solution, and detecting the fluorescence intensity after adding the heme standard solution; and taking the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as the ordinate, taking the concentration of the heme standard solution as the abscissa, and performing linear fitting to obtain a standard curve.
In the present invention, the molar ratio of artemisinin to thiamine is preferably in accordance with the requirements defined in the above scheme and will not be described in further detail herein. The limitations of the detection method of the artemisinin, the thiamine, the inorganic base, the EDTA and the fluorescence intensity are consistent with the requirements, and are not repeated herein.
The conditions for the incubation according to the present invention are preferably identical to those described in the above-mentioned protocol and will not be described herein.
Obtaining the fluorescence intensity (F) after adding the heme standard solution, and obtaining the initial fluorescence intensity (F) of the mixed solution of the artemisinin and the thiamine and the fluorescence intensity (F) after adding the heme standard solution0) Fitting a standard equation by taking the difference (delta F) of the standard solution of the heme as a dependent variable and the concentration (c) of the standard solution of the heme as an independent variable, and calculating according to the standard equation to obtain the standard equation to be treatedAnd measuring the concentration of the heme in the heme solution.
In order to further illustrate the present invention, the following examples are provided to describe the method for detecting the concentration of hemoglobin solution in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
mu.L EDTA (0.1mol/L), 10. mu.L artemisinin (5mmol/L), 10. mu.L heme standard solutions of different concentrations (0.002,0.005,0.01,0.05,0.1,0.2,0.3,0.5,0.8,2.0,5.0, 10.0. mu. mol/L) and 100. mu.L thiamine (1mmol/L) were added sequentially to 880. mu.L sodium hydroxide solution (0.1mol/L), incubated at 25 ℃ for 20 minutes and then subjected to fluorescence spectroscopy before the addition of the standard solution with heme (F1 mol/L)0) Fitting a standard curve according to the linear relation between the change value (delta F) of the fluorescence intensity of the hemoglobin and the concentration of the hemoglobin, wherein the standard curve is shown in a graph 1-2, and the graph 1 shows the change of the fluorescence intensity after the hemoglobin standard solutions with different concentrations are added; the concentration of the heme standard solution is 0, 0.002,0.005,0.01,0.05,0.1,0.2,0.3,0.5,0.8,2.0,5.0 and 10.0 mu mol/L once from bottom to top, the relationship between the fluorescence intensity of the system and the concentration of heme is shown in FIG. 2, the interpolation graph is the standard equation of the fluorescence intensity change and the heme, the obtained standard equation is DeltaF ═ 32.01+23.54c (c is the concentration of the heme and has the unit of nmol/L), the linear coefficient is 0.9954, the linear range is 2.0-300.0 nmol, and the detection limit is 0.68 nmol/L.
Mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water to obtain a mixed solution to be detected, incubating the mixed solution to be detected under the same incubation condition to obtain an incubated solution to be detected, detecting the fluorescence intensity of the incubated solution to be detected, calculating the concentration of heme in the heme solution to be detected according to a standard equation, and repeatedly measuring the heme solution of 300nmol/L for 6 times, wherein the relative standard deviation is 6.8%.
Example 2
The method is the same as the example 1, except that the hemoglobin solution to be detected is replaced by the solution containing cobalt ions, magnesium ions, copper ions, zinc ions, iron ions, glucose, phenylalanine, leucine, glycine, alanine and cysteine, and the fluorescence spectrum measurement is performed on the hemoglobin solution to be detected, and the result is shown in fig. 3, and as can be seen from fig. 3, the fluorescence intensity of the system is not obviously changed after the substances are added, and the fluorescence intensity of the system is obviously enhanced only after the hemoglobin is added, so that the detection method shows better selectivity and is not interfered by some common metal ions and biomolecules.
The foregoing is merely a preferred embodiment of the invention and is not intended to limit the invention in any manner. It should be noted that, for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (8)
1. A method for detecting the concentration of a heme solution comprises the following steps:
(1) mixing a heme solution to be detected, disodium ethylene diamine tetraacetate, artemisinin, thiamine, inorganic base and water, incubating the obtained mixed solution to be detected to obtain an incubation solution to be detected, and detecting the fluorescence intensity of the incubation solution to be detected;
(2) obtaining the concentration of heme in the heme solution to be detected according to the fluorescence intensity of the incubation solution to be detected obtained in the step (1) and a preset standard curve; the standard curve takes the concentration of the heme standard solution as an abscissa and the difference value of the fluorescence intensity after the heme standard solution is added and the initial fluorescence intensity of the artemisinin and thiamine mixed solution as an ordinate.
2. The detection method according to claim 1, wherein the incubation temperature in the step (1) is 15 to 40 ℃, and the incubation time is 5 to 30 min.
3. The detection method according to claim 1, wherein the molar ratio of artemisinin to thiamine in the mixed solution to be detected in the step (1) is 10-2000: 0.5-500.
4. The detection method according to claim 1, wherein the inorganic base in the step (1) is sodium hydroxide or potassium hydroxide.
5. The detection method according to claim 1 or 4, wherein the inorganic base is added in the step (1) in the form of an inorganic base solution, and the concentration of the inorganic base solution is 5 to 500 mmol/L.
6. The detection method as claimed in claim 1, wherein the artemisinin in the step (1) is added in the form of artemisinin solution, and the concentration of the artemisinin solution is 0.05-50 mmol/L.
7. The detection method according to claim 1, wherein thiamine is added in the form of a thiamine solution having a concentration of 5 μmol/L to 2mmol/L in step (1).
8. The detection method according to claim 1, wherein the linear range of the standard curve in the step (2) is 2.0-300.0 nmol/L.
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| CN107677658A (en) * | 2017-10-10 | 2018-02-09 | 广西师范学院 | Utilize the method for hemoglobin concentration in nitrogen-doped carbon quantum dots characterization solution |
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| WO2010009459A1 (en) * | 2008-07-18 | 2010-01-21 | Bayer Healthcare Llc | Methods, devices, and systems for glycated hemoglobin analysis |
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|---|---|---|---|---|
| CN102370993A (en) * | 2010-08-23 | 2012-03-14 | 王革 | Preparation method for novel red blood cell substitute-artificial red blood cell fluorescent nanoparticles |
| JP2012130505A (en) * | 2010-12-21 | 2012-07-12 | Fujifilm Corp | Light measurement system and light measurement method |
| CN103293145A (en) * | 2013-05-16 | 2013-09-11 | 赫利森（厦门）生物科技有限公司 | Chemiluminescence reagent |
| CN107677658A (en) * | 2017-10-10 | 2018-02-09 | 广西师范学院 | Utilize the method for hemoglobin concentration in nitrogen-doped carbon quantum dots characterization solution |
Non-Patent Citations (2)
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