CN108318276A - A kind of osculum liquid nitrogen container Special sample collection device and application method - Google Patents
A kind of osculum liquid nitrogen container Special sample collection device and application method Download PDFInfo
- Publication number
- CN108318276A CN108318276A CN201810017901.XA CN201810017901A CN108318276A CN 108318276 A CN108318276 A CN 108318276A CN 201810017901 A CN201810017901 A CN 201810017901A CN 108318276 A CN108318276 A CN 108318276A
- Authority
- CN
- China
- Prior art keywords
- outer tube
- liquid nitrogen
- osculum
- holder
- inner tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
Abstract
The invention discloses a kind of osculum liquid nitrogen container Special sample collection device and application methods, it includes outer tube, inner tube, holder and liner, and outer tube, which has, is securable to the icv fixed mechanism of osculum liquid nitrogen container；Inner tube activity is nested in pipe outside, and there are annular spaces between inner and outer tubes, and inner tube can move up and down in outer tube along its axis direction；Holder includes along more circumferentially distributed spokes of inner tube bottom end, and the top of each spoke and the bottom end of inner tube are hinged, and spring is both provided on the outside of each spoke, and one end of spring and the bottom end of outer tube connect, the bottom end connection of the corresponding spoke of the other end；Liner is covered in the inside of holder, and is provided with the through-hole being only connected with inner tube in the middle part of liner；Under normal condition, in outer tube, spring is in nature retracted position for holder, liner and spring contraction；Under use state, holder stretches out the bottom end of outer tube, and spring is in tensional state, and it is in umbrella-shaped structure to drive holder and liner expansion.
Description
Technical field
The present invention relates to a kind of osculum liquid nitrogen container Special sample collection device and application methods, belong to molecular biology neck
Domain.
Background technology
With the rapid development in agri-scientific research field, requirement of the researcher to molecular biology sample is also higher and higher, liquid
The use scope of nitrogen is more and more wider, and liquid nitrogen container is also varied.Sample collection distance location laboratory is farther out or single needs
When acquiring a large amount of Plant tissue samples, for a large amount of sample loss caused by volatilization of liquid nitrogen during down-sampling, partly grind
The person of studying carefully favors in osculum liquid nitrogen container.It is narrow due to filling mouth, and neck tube is longer, has angle with inner tank wall, can not directly pour out
Sample, it is necessary to it is used to collect the device of sample using mating pail or the string bag etc., and these mating devices are all smaller, take
Sample amount is just restricted.
Invention content
In view of the above-mentioned problems, the object of the present invention is to provide a kind of osculum liquid nitrogen container Special sample collection device and users
Method, the device can facilitate the taking-up of sample in osculum liquid nitrogen container, are not required to the sampling kit such as pail or string bag, make small oral fluid
Nitrogen tank is suitable for remote sampling and larger sampling quantity, avoid using wide-mouth liquid nitrogen container caused by liquid nitrogen loses sample quality by
The problems such as damage.
To achieve the above object, the present invention uses following technical scheme：A kind of osculum liquid nitrogen container Special sample collection device,
It is characterized in that, it includes outer tube, inner tube, holder and liner, the outer tube is icv with osculum liquid nitrogen container is securable to
Fixed mechanism；Said inner tube activity is nested in the outer tube, there are annular space between said inner tube and outer tube, and it is described in
Pipe can move up and down in the outer tube along its axis direction；The holder includes along circumferentially distributed more in said inner tube bottom end
Spoke, the top of each spoke and the bottom end of said inner tube are hinged, while being both provided with bullet on the outside of each spoke
Spring, one end of the spring are connect with the bottom end of the outer tube, the bottom end connection of the corresponding spoke of the other end；
The liner is covered in the inside of the holder, and is provided with the through-hole being only connected with said inner tube in the middle part of the liner；Just
Under normal state, in the outer tube, the spring is in nature retracted position for the holder, liner and spring contraction；It uses
Under state, the holder stretches out the bottom end of the outer tube, and the spring is in tensional state, and the holder and liner is driven to be unfolded
In umbrella-shaped structure.
The device further includes that limitation said inner tube continues the holding mechanism moved up and down in the outer tube, so that the branch
Frame and liner keep umbrella-shaped structure expansion.
The holding mechanism is the mutually matched card shelves being arranged on the outer tube wall and said inner tube outer wall, makes institute
Movable buckle between outer tube and said inner tube is stated to connect.
The card shelves are unidirectional setting, wherein the card shelves include sawtooth buckle, head of stumbling, spring and spanner, in said inner tube
Outer wall on along its length interval multiple sawtooth buttons are set, be flexibly connected on the inner wall close to the outer tube top
The head of stumbling, and the end anchors of the head of stumbling are between two sawtooth buttons, in end close to the head of stumbling and described
The spring is connected between the inner wall of outer tube, one end of the spanner is connect with the head of stumbling, and the other end vertically extends described outer
The top of pipe.
The fixed mechanism is the hook being arranged on the outer tube top.
The outer tube, inner tube and holder are all made of cold-resistant stainless steel, and the liner uses soft low temperature material processed, such as
Cotton, the hook use metal material.
The liner uses cotton.
The present invention also provides a kind of application methods based on osculum liquid nitrogen container Special sample collection device comprising following
Step：
1) osculum liquid nitrogen container Special sample collection device is stretched into along the tank mouth of osculum liquid nitrogen container in tank body；
2) outer tube is fixed in the neck tube of osculum liquid nitrogen container by fixed mechanism, then pushes inner tube along the axis of outer tube
Direction slide downward, holder stretch out the bottom end of outer tube, and spring gradually trails, storage tool elastic potential energy, bottom end of the spring to spoke
With along the upward active force in spoke length direction, driving holder and liner expansion to be in umbrella-shaped structure, until the end of holder with
When the inner wall fitting of osculum liquid nitrogen container, stop pushing inner tube；
3) lifting osculum liquid nitrogen container makes its tank mouth downward, and the inner cavity of sample and liquid nitrogen through inner tube in osculum liquid nitrogen container drains into
One cool-bag quickly picks up all samples with common strainer, is transferred to -80 DEG C of refrigerator freezings, completes the collection of sample, pack up
Entire collection device, the liquid nitrogen recycling in cool-bag.
The present invention uses above technical scheme, has the following advantages that：1, the special sample of osculum liquid nitrogen container provided by the invention
Product collection device, whole device is stretched into liquid nitrogen container, and inner tube is pushed to be moved downward along outer tube axis direction, spring stretching, extension storage tool
Elastic potential energy drives entire holder and liner to be unfolded to be in umbrella-shaped structure, can be conveniently accomplished the receipts of sample in osculum liquid nitrogen container
Collection is not required to the sampling kit such as pail or string bag, realizes and sampled and stored at a distance larger adopt using osculum liquid nitrogen container
Sample amount, avoid using wide-mouth liquid nitrogen container sample quality is impaired caused by liquid nitrogen loses the problems such as.2, osculum provided by the invention
Liquid nitrogen container Special sample collection device further includes that limitation inner tube continues the holding mechanism moved up and down in outer tube, so that holder
Umbrella-shaped structure expansion, staff is kept only to need to hold up osculum liquid nitrogen container, so that the outlet of inner tube is tilted down and pour out sample with liner
Product and liquid nitrogen can avoid the problem that staff contacts liquid nitrogen and frostbite occurs while facilitating sample collection, improve entire
The processing safety of device.3, osculum liquid nitrogen container Special sample collection device provided by the invention, outside outer tube wall and inner tube
The mutually matched card shelves being arranged on wall make movable buckle between outer tube and inner tube connect, realize the expansion width of holder and liner
Spend it is adjustable, make collection device be suitable for different inner diameters size osculum liquid nitrogen container, expand the scope of application of whole device.4, this hair
The osculum liquid nitrogen container Special sample collection device of bright offer, card shelves between outer tube and inner tube are that sawtooth is buckled, head of stumbling, spring and pulled
The unidirectional card shelves of hand composition, make inner tube unidirectionally move downward, when guarantee pours out sample along the inner cavity of inner tube, between inner and outer tubes
It is firm snap connection, improve whole device use reliability.5, outer tube, inner tube and holder of the invention are all made of resistance to
Cold stainless steel, for liner using the soft low temperature material processed such as cotton, hook uses metal material, the purchase of each building block at
This low and purchasing channel is extensive, reduces the cost of whole device, improves the practicability of whole device and be conducive to whole device
It promotes and applies.
Description of the drawings
Fig. 1 is the overall structure diagram of the present invention；
Fig. 2 is the partial enlargement structural representation between outer tube of the present invention and inner tube.
Specific implementation mode
The present invention is described in detail below with reference to the accompanying drawings and embodiments.
As shown in Figure 1, the present invention provides a kind of osculum liquid nitrogen container Special sample collection device, the device include outer tube 1,
Inner tube 2, hook 3, holder 6, liner 7 and spring 8, in the top of outer tube 1 setting hook 3,2 activity of inner tube is nested in pipe 1 outside,
There are annular spaces between inner tube 2 and outer tube 1, and inner tube 2 can relatively move in outer tube 1 above and below its axis direction；Branch
Frame 6 includes along more circumferentially distributed spokes 61 of 2 bottom end of inner tube, and the top of each spoke 61 is hinged with the bottom end of inner tube 2, simultaneously
It is both provided with a spring 8 in 61 outside of each spoke, one end of spring 8 is connect with the bottom end of outer tube 1, and the other end is in contrast
The bottom end for the spoke 61 answered connects；Covering liner 7 is fixed in the inside of holder 6,7 middle part of liner, which is provided with, to be only connected with inner tube 2
Logical through-hole；Under normal condition, holder 6, liner 7 and spring 8 are contracted in outer tube 1, and spring 8 is in nature retracted position；Make
With under state, holder 6 stretches out the bottom end of outer tube 1, and spring 8 gradually trails, storage tool elastic potential energy, bottom of the spring 8 to spoke 61
End has along the upward active force of 61 length direction of spoke, is in umbrella-shaped structure to drive entire holder 6 and liner 7 to be unfolded；It should
Device further includes that limitation inner tube 2 continues the holding mechanism moved up and down in outer tube 1, so that holder 6 and liner 7 keep umbrella knot
Structure is unfolded.
Further, it is in 1 inner wall of outer tube and inner tube 2 that limitation inner tube 2 continues the holding mechanism moved up and down in outer tube 1
The mutually matched card shelves being arranged on outer wall make movable buckle between outer tube 1 and inner tube 2 connect, in this way, can both limit inner tube
2 continue to slide up and down in outer tube 1, and it is in umbrella-shaped structure in osculum liquid nitrogen container 13 to make holder 6 and liner 7, and can be according to osculum
The inner diameter size of liquid nitrogen container is different, and inner tube 2 is slided down to different cards file location, makes the expansion amplitude of holder 6 and liner 7 not
Together, to be applicable in the osculum liquid nitrogen container of different inner diameters size.
Further, the card shelves between the inner wall of outer tube 1 and the outer wall of inner tube 2 are unidirectional setting, wherein card shelves include saw
Tooth button 9, stumble first 10, spring 11 and spanner 12, multiple sawtooth buttons 9 are arranged in interval along its length on the outer wall of inner tube 2,
Flexible connection one stumbles first 10 on the inner wall on 1 top of outer tube, and stumble first 10 end anchors between two sawtooth buttons 9,
Close to stumble first 10 end and the inner wall of outer tube 1 between connecting spring 11, one end of spanner 12 and stumbling first 10 connect, and the other end erects
The straight top for stretching out outer tube 1, under normal condition, first 10 head of stumbling is buckled between two sawtooth buttons 9；In use, in pushing
2 slide downward of pipe, due to stumble first 10 by sawtooth button 9 downward pressure, make spring 11 shrink, drive stumble first 10 end move
Go out between two sawtooth buttons 9,2 slide downward of inner tube, at this point, spring 11 is sprung back, first 10 end of making to stumble is buckled in other two again
Between sawtooth button 9, this process repeats when inner tube 2 constantly moves downward, until inner tube 2 stops after moving to suitable position；
After being finished, only spanner 12 is moved to 2 side of inner tube, so that spring 11 is squeezed to 1 inner wall direction of outer tube and shrink, drive head of stumbling
The inner wall side movement of 10 end faces outwards pipe 1 just can pull inner tube 2 along outer tube 1 until between two sawtooth buttons 9 of its end removal
Holder 6, liner 7 and spring 8 are withdrawn in outer tube 1, otherwise pull up inner tube 2, Zhi Nengshi by force by axis direction upward sliding
The more draw the more tight.
Further, outer tube 1, inner tube 2 and holder 6 are all made of cold-resistant stainless steel, and liner 7 is using the soft low temperature resistant material of system
Material, such as cotton, hook 3 use metal material.
Based on a kind of above-mentioned osculum liquid nitrogen container Special sample collection device, the present invention also provides a kind of osculum liquid nitrogen containers
The application method of Special sample collection device comprising following steps：
1) osculum liquid nitrogen container Special sample collection device is stretched into along the tank mouth of osculum liquid nitrogen container 13 in tank body；
2) hook 3 is hung in the tank mouth of osculum liquid nitrogen container 13, outer tube 1 is made to be fixed in the neck tube of osculum liquid nitrogen container 13,
Then push inner tube 2 along the axis direction slide downward of outer tube 1, holder 6 stretches out the bottom end of outer tube 1, and spring 8 gradually trails,
Storage tool elastic potential energy, spring 8 have along 61 length direction of spoke upward active force the bottom end of spoke 61, drive 6 He of holder
The expansion of liner 7 is in umbrella-shaped structure, until when the end of holder 6 is bonded with the inner wall of osculum liquid nitrogen container 13, stops pushing inner tube 2；
3) lifting osculum liquid nitrogen container 13 makes its tank mouth downward, the inner cavity of sample and liquid nitrogen through inner tube 2 in osculum liquid nitrogen container 9
A cool-bag is drained into, all samples is quickly picked up with common strainer, is transferred to -80 DEG C of refrigerator freezings, complete the collection of sample,
Entire collection device is packed up, the liquid nitrogen recycling in cool-bag.
The present invention is only illustrated with above-described embodiment, and structure, installation position and its connection of each component are all can have
Changed.Based on the technical solution of the present invention, all improvement that individual part is carried out according to the principle of the invention or equivalent
Transformation, should not exclude except protection scope of the present invention.
Claims (8)
1. a kind of osculum liquid nitrogen container Special sample collection device, which is characterized in that it includes：
Outer tube, the outer tube, which has, is securable to the icv fixed mechanism of osculum liquid nitrogen container；
Inner tube, said inner tube activity are nested in the outer tube, there are annular space between said inner tube and outer tube, and it is described in
Pipe can move up and down in the outer tube along its axis direction；
Holder, the holder include along more circumferentially distributed spokes of said inner tube bottom end, the top of each spoke and institute
The bottom end for stating inner tube is hinged, while spring is both provided on the outside of each spoke, one end of the spring and the outer tube
Bottom end connection, the corresponding spoke of the other end bottom end connection；
Liner, the liner is covered in the inside of the holder, and is provided in the middle part of the liner and is only connected with said inner tube
Through-hole；
Under normal condition, in the outer tube, the spring is in nature scale shape for the holder, liner and spring contraction
State；Under use state, the holder stretches out the bottom end of the outer tube, and the spring is in tensional state, drive the holder and
Liner expansion is in umbrella-shaped structure.
2. a kind of osculum liquid nitrogen container Special sample collection device as described in claim 1, it is characterised in that：It further include limitation institute
It states inner tube and continues the holding mechanism moved up and down in the outer tube, so that the holder and liner keep umbrella-shaped structure expansion.
3. a kind of osculum liquid nitrogen container Special sample collection device as claimed in claim 2, it is characterised in that：The holding mechanism
For the mutually matched card shelves being arranged on the outer tube wall and said inner tube outer wall, make between the outer tube and said inner tube
Movable buckle connects.
4. a kind of osculum liquid nitrogen container Special sample collection device as claimed in claim 3, it is characterised in that：The card shelves are single
To setting, wherein the card shelves include sawtooth buckle, head of stumbling, spring and spanner, on the outer wall of said inner tube along its length
Multiple sawtooth buttons are arranged in interval, stumble described in flexible connection on the inner wall close to the outer tube top head, and the head of stumbling
End anchors between two sawtooth buttons, connecting institute between the end and the inner wall of the outer tube of the head of stumbling
Spring is stated, one end of the spanner is connect with the head of stumbling, and the other end vertically extends the top of the outer tube.
5. a kind of osculum liquid nitrogen container Special sample collection device as described in claim 1, it is characterised in that：The fixed mechanism
For the hook for being arranged on the outer tube top.
6. such as a kind of osculum liquid nitrogen container Special sample collection device described in any one of claim 1 to 5, it is characterised in that：Institute
It states outer tube, inner tube and holder and is all made of cold-resistant stainless steel, the liner uses soft low temperature material processed, such as cotton described
Hook uses metal material.
7. a kind of osculum liquid nitrogen container Special sample collection device as claimed in claim 6, it is characterised in that：The liner uses
Cotton.
8. a kind of application method based on osculum liquid nitrogen container Special sample collection device described in claim 1, which is characterized in that
It includes the following steps：
1) osculum liquid nitrogen container Special sample collection device is stretched into along the tank mouth of osculum liquid nitrogen container in tank body；
2) outer tube is fixed in the neck tube of osculum liquid nitrogen container by fixed mechanism, then pushes inner tube along the axis direction of outer tube
Slide downward, holder stretch out the bottom end of outer tube, and spring gradually trails, and storage tool elastic potential energy, spring has the bottom end of spoke
The active force upward along spoke length direction, it is in umbrella-shaped structure to drive holder and liner expansion, until the end of holder and osculum
When the inner wall fitting of liquid nitrogen container, stop pushing inner tube；
3) lifting osculum liquid nitrogen container makes its tank mouth downward, and the inner cavity of sample and liquid nitrogen through inner tube in osculum liquid nitrogen container drains into a guarantor
Warm container quickly picks up all samples with common strainer, is transferred to -80 DEG C of refrigerator freezings, completes the collection of sample, packs up entire
Collection device, the liquid nitrogen recycling in cool-bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810017901.XA CN108318276B (en) | 2018-01-09 | 2018-01-09 | Special sample collecting device for small-opening liquid nitrogen tank and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810017901.XA CN108318276B (en) | 2018-01-09 | 2018-01-09 | Special sample collecting device for small-opening liquid nitrogen tank and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108318276A true CN108318276A (en) | 2018-07-24 |
| CN108318276B CN108318276B (en) | 2020-04-07 |
Family
ID=62894022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810017901.XA Active CN108318276B (en) | 2018-01-09 | 2018-01-09 | Special sample collecting device for small-opening liquid nitrogen tank and using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108318276B (en) |
Citations (6)
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| CN206176032U (en) * | 2016-11-16 | 2017-05-17 | 中国长江三峡集团公司中华鲟研究所 | A liquid nitrogen container for sampling |
| CN107114359A (en) * | 2017-06-16 | 2017-09-01 | 中国科学院西北高原生物研究所 | The both-side opening storage bag and preparation method of animal tissue's sample in a kind of liquid nitrogen container |
| CN206695465U (en) * | 2017-04-01 | 2017-12-01 | 北京焕生汇生物技术研究院 | Liquid nitrogen container pail is used in one kind storage |
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2018
- 2018-01-09 CN CN201810017901.XA patent/CN108318276B/en active Active
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| KR20160010183A (en) * | 2014-07-18 | 2016-01-27 | 메디칸(주) | Freezer for storage of human tissue |
| CN203985782U (en) * | 2014-08-14 | 2014-12-10 | 中国烟草总公司郑州烟草研究院 | With the wide-mouth liquid nitrogen container of net formula moving liner |
| CN204489594U (en) * | 2015-01-22 | 2015-07-22 | 中国热带农业科学院南亚热带作物研究所 | A kind of portable access sample liquid nitrogen container |
| CN206176032U (en) * | 2016-11-16 | 2017-05-17 | 中国长江三峡集团公司中华鲟研究所 | A liquid nitrogen container for sampling |
| CN206695465U (en) * | 2017-04-01 | 2017-12-01 | 北京焕生汇生物技术研究院 | Liquid nitrogen container pail is used in one kind storage |
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| CN108318276B (en) | 2020-04-07 |
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