CN108308950A - A kind of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV bactericidal effect - Google Patents
A kind of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV bactericidal effect Download PDFInfo
- Publication number
- CN108308950A CN108308950A CN201810198783.7A CN201810198783A CN108308950A CN 108308950 A CN108308950 A CN 108308950A CN 201810198783 A CN201810198783 A CN 201810198783A CN 108308950 A CN108308950 A CN 108308950A
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- Prior art keywords
- compressor
- short wavelength
- supporter
- preserving fruit
- vegetable utilizing
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Classifications
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47F—SPECIAL FURNITURE, FITTINGS, OR ACCESSORIES FOR SHOPS, STOREHOUSES, BARS, RESTAURANTS OR THE LIKE; PAYING COUNTERS
- A47F3/00—Show cases or show cabinets
- A47F3/04—Show cases or show cabinets air-conditioned, refrigerated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
Abstract
The invention discloses a kind of preserving fruit and vegetable utilizing showcases that can enhance short wavelength UV bactericidal effect, including show area and compressor room.Show area includes glass cabinet door, UV C ultraviolet germicidals, supporter, UV C ultraviolet germicidals fixing devices and supporter fixing groove.Glass cabinet door is characterized as that selectivity penetrates red visible;Show area is built-in with several supporters, and supporter is hollow out;Bactericidal lamp specifically uses UV C ultraviolet lamps, is mounted on supporter.The indoor compressor of compressor, condenser collectively form refrigeration system with the evaporator, fan for being located at rear in the show area;Temperature control device in compressor realizes that temperature is adjusted with compressor linkage;The operation display area of temperature control device includes display screen and thermostat button, and display screen and thermostat button are located at the outer surface of compressor room;It is additionally provided with distribution box in compressor room, passes through distribution box distributor circuit.The present invention is by UV C ultraviolet germicidals and is subject to selectivity through feux rouges processing, significantly improves the germicidal efficiency that UV C cause fruits and vegetables maize ear rot fungal pathogens.
Description
Technical field
The invention belongs to postharvest technology of fruits and vegetables fields, and in particular to a kind of fruits and vegetables guarantor that can enhance short wavelength UV bactericidal effect
Fresh showcase reduces rot loss of fruit and vegetable food during sales exhibition for killing fruit and vegetable surfaces disease fungus.
Background technology
Fruit and vegetable provides essential nutriment for the mankind, affects the health of the mankind.Fruits and vegetables harvest
It is easily infected later by disease fungus, cause to rot and leads to heavy losses.Currently, in order to prevent fruits and vegetables disease fungus, chemistry
Pesticide is still now widely used measure.It is new there is an urgent need for developing since health problem caused by pesticide residue becomes increasingly conspicuous
Type, clean and effective fruit and vegetable preserving method.
UV-C be wavelength be 200-280nm short wave ultraviolet light, because its energy height, safety, cleaning, it is efficient the advantages that it is current
It is had a wide range of applications in disinfection cupboard, Sterilizing clothes cabinet etc..But for fresh fruit of vegetables, UV-C is for fruits and vegetables tissue cell
May have certain adverse effect, research shows that exposure dose it is excessively high UV-C and UV-C irradiation times it is long, may cause
The problems such as fruit-vegetable quality declines, disease resistance reduces, therefore seriously hinder applications of the UV-C in terms of antiseptic preservation of fruits and vegetables.
There is UV-C good bactericidal effect, mainly UV-C can make to form pyrimidine on microbial DNA between adjacent base
Dimer, and make the DNA damage of disease fungus, to hinder the duplication of its DNA, the transcription of RNA and the synthesis of protein, most
The growth and development for influencing disease fungus eventually is even dead.And disease fungus itself also has the mechanism that DNA plerosis damages, to be resistant to
The stress of UV-C, if light repairs approach, it is the pyrimidine dimer for causing DNA damage for ultraviolet light and being formed, in pars affecta
The reparation approach that position is repaired, it is by DNA photodissociation enzymatics, and under visible light, catalysis pyrimidine dimer forms monomer;Also
Another selective ablation repairs approach, identifies and cut off damage position as the first step by UV nucleic acid damages restriction endonuclease (UVDE)
Point is then repaired DNA by a series of DNA desmoenzymes, DNA ligase etc..Fungi also leads to UV- to the tolerance of UV-C
Applications of the C in terms of antiseptic preservation of fruits and vegetables is seriously hampered.
Studies have shown that giving dark processing after fruits and vegetables UV-C irradiations, the sterilizing ability of UV-C, and cause of disease can be significantly improved
The ability that fungi is resistant to UV-C is mainly related with the process of its photoresponse.Fungi blue light receptor (WCC) is that main photoresponse is held
Load person, it is the transcription factor of a GATA Zinc finger domain, has the LOV structural domains that can incude light, and have and can play
The zinc finger protein structural domain of downstream gene expression.Blue light receptor downstream can induce fungal DNA photodissociation enzyme gene and UVDE genes
Expression, to enhance the ability of the damage of fungi DNA plerosis and tolerance UV-C radiation.However, the inductive effect is only in blue light and white
Under light effectively, under feux rouges and dark condition, photodissociation enzyme gene and UVDE genes are unable to induced expression.Therefore, disease fungus
The tolerance of UV-C is enhanced under blue light and white light, and to UV-C hypersensitizations under the conditions of dark and feux rouges.Based on this original
Reason, the present invention provides a kind of safe and environment-friendly, cleaning, the efficient antiseptic preservation of fruits and vegetables exhibitions for killing fruit and vegetable surfaces disease fungus
Show cabinet, effectively reduces the UV-C irradiation times killed needed for disease fungus spore, avoid UV-C is excessively used to greatest extent
The damage that fruit and vegetable food itself is brought.The antistaling cabinet also has visual speciality, convenient for fresh fruit of vegetables product in shelf life
Use the technology.
Invention content
The technical problem to be solved in the present invention is to provide a kind of fruits and vegetables guarantors that can enhance short wavelength UV (UV-C) bactericidal effect
Fresh showcase, by UV-C ultraviolet germicidals, red glass shows cabinet door, cryogenic refrigerating system, is not damaging fruits and vegetables and not
Under conditions of influencing merchandise display effect, the dosage needed for UV-C sterilizations is greatly reduced, UV-C is improved and maize ear rot is caused to fruits and vegetables
The germicidal efficiency of fungal pathogens is easy to use and promotes.
In order to solve the above technical problems, the present invention uses following technical scheme:
The present invention proposes a kind of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV UV-C bactericidal effects, including show area
And compressor room;Wherein, the show area includes glass cabinet door, UV-C ultraviolet germicidals, supporter, and UV-C ultraviolet germicidals are solid
Determine slot and supporter fixing groove;The UV-C ultraviolet germicidals fixing groove is arranged on the supporter;The UV-C is ultraviolet to be killed
Bacterium lamp is hung in the UV-C ultraviolet germicidals fixing groove;The compressor room includes refrigeration system, temperature regulating device and distribution
Case.
In the present invention, the glass cabinet door only selectively penetrates red visible.
In the present invention, the supporter is engraved structure.
In the present invention, it is respectively provided in every layer of UV-C ultraviolet germicidals fixing groove there are one UV-C ultraviolet germicidals.
In the present invention, the UV-C ultraviolet germicidals are 15W.
In the present invention, the refrigeration system includes:Compressor, condenser and evaporator and fan, the evaporator are located at
Below the fan.
In the present invention, the temperature regulating device in the compressor realizes that temperature is adjusted with compressor linkage, can be according to need
It is 0~16 DEG C to adjust in-cabinet temperature.
In the present invention, the operation display area of the temperature control device includes temperature adjustment display screen and thermostat button, the display screen
It is located at the outer surface of the compressor room with thermostat button.
The present invention has the advantages that:The present invention shows cabinet door, low temperature by UV-C ultraviolet germicidals, red glass
Refrigeration system had not only ensured the fresh-keeping of fruits and vegetables, but also UV-C had minimized the damage of fruits and vegetables, and was not influencing merchandise display effect
Under conditions of fruit, the germicidal efficiency that UV-C causes fruits and vegetables maize ear rot fungal pathogens is improved.
Description of the drawings:
Fig. 1 is a kind of agent structure signal of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV bactericidal effect of the present invention
Figure.
Fig. 2 is a kind of side structure signal of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV bactericidal effect of the present invention
Figure.
Fig. 3 is a kind of plan structure signal of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV bactericidal effect of the present invention
Figure.
Fig. 4 botrytis cinereas (Botrytis cinerea) are resistant to the comparison of UV-C abilities under different light medium.Note:(a) grey mold
Bacterium spore germination figure (bar=100 μm);(b) botrytis cinerea spore germination rate;(c) botrytis cinerea spore germination length of tube.
Fig. 5 moulds are resistant to the comparison of UV-C abilities under different light medium.Note:(a) mould spore germination figure (bar=100 μ
m);(b) Penicillium digitatum (Penicillium digitatum) spore germination rate;(c) mould spore germination length of tube.
Fig. 6 colletotrichum gloeosporioides Penzs (Colletotrichum gloeosporioides) are resistant to UV-C abilities under different light medium
Comparison.Note:(a) colletotrichum gloeosporioides Penz spore germination figure (bar=100 μm);(b) colletotrichum gloeosporioides Penz spore germination rate;(c) glue spore
Anthrax spores sprout length of tube.
Causative effect compares after the botrytis cinerea of grape is infected in Fig. 7 UV-C processing.(a) pathogenic design sketch;(b) pathogenic data
Analysis.
Specific implementation mode:
In conjunction with following specific examples and attached drawing, the invention will be described in further detail.Implement process, the item of the present invention
Part, experimental method etc. are among the general principles and common general knowledge in the art in addition to the following content specially referred to, the present invention
Content is not particularly limited.
The show areas 1-;2-UV-C ultraviolet germicidals;3- glass cabinet doors;4- supporters;5-UV-C ultraviolet germicidal fixing grooves;
6- supporter fixing grooves;7- thermostat buttons;8- temperature adjustment display screens;9- compressor room;10- fans;11- evaporators;12- distribution
Case;13- condensers;14- compressors.
In the present invention, showcase is made of show area 1 and compressor room 9.Wherein, the show area 1 includes UV-C ultraviolet
Bactericidal lamp 2, glass cabinet door 3, supporter 4, UV-C ultraviolet germicidals fixing groove 5 and supporter fixing groove 6;The compressor room 9
Including refrigeration system, temperature regulating device and distribution box 12.
In present example, fruits and vegetables cause maize ear rot fungal pathogens to be resistant to the significant decrease of UV-C abilities, therefore the glass under feux rouges
Glass cabinet door 3 is characterized as that selectivity penetrates red visible.
In the present invention, the supporter 4 is hollow out, it is ensured that entire showcase is completely covered in UV-C.
In the present invention, supporter fixing groove 6 is equipped in the showcase, supporter can adjust position according to food size is placed
It sets.
In the present invention, UV-C ultraviolet germicidals fixing groove 5 is equipped in the showcase, fixing groove is located on supporter 4, can
Fixed UV-C ultraviolet germicidals.
In the present invention, the UV-C ultraviolet germicidals 2,15W are hung in UV-C ultraviolet germicidals fixing groove 5, one layer one
A UV-C ultraviolet germicidals, it is ensured that UV-C covers all around showcase.
In the present invention, the refrigeration system is the steaming of compressor indoor compressor 14, condenser 13 and show area rear
Hair device 11, fan 10 collectively form.
In the present invention, the evaporator 11 is located at the lower section of fan 10, by fan blowing by the low-temperature airflow of evaporator from
Top to bottm flows to show area everywhere.
In the present invention, the operation display area of the temperature control device includes temperature adjustment display screen 8 and thermostat button 7, the temperature adjustment
Display screen 8 and thermostat button 7 are located at the outer surface of the compressor room 9.
In the present invention, the temperature regulating device in the compressor realizes that temperature is adjusted with the linkage of affiliated compressor, can be according to need
It is 0~16 DEG C to adjust in-cabinet temperature.
In the present invention, the distribution box 12 is located in compressor room 9, passes through the distribution box distributor circuit.
The tolerance of UV-C is enhanced under blue light and white light according to postharvest pathogenic fungi, and in dark and feux rouges condition
Under to this principle of UV-C hypersensitizations, i.e., blue light can start postharvest pathogenic fungi tolerance UV-C key gene DNA photodissociation enzyme genes
With the expression of UVDE genes, UV-C abilities are resistant to enhance it;And the inductive effect is effective only under blue light and white light,
Under feux rouges and dark condition, photodissociation enzyme gene and UVDE genes are unable to induced expression.The present invention is based on this principles, pass through UV-C
Dark is replaced with feux rouges after processing, the effect that light is resistant to postharvest pathogenic fungi UV-C is reduced to greatest extent, extends fruit
The fresh keeping time of vegetable, screening fruits and vegetables can not only be visualized by reaching, but also can control the effect of disease.
The present invention proves the present invention by the way that three kinds of representative postharvest pathogenic fungis are resistant to the capability analysis of UV-C
The effect that the method is inoculated on fruit is tested for the generality of postharvest pathogenic fungi, and by representative of botrytis cinerea, it was demonstrated that this
The feasibility of invention.
Embodiment 1, botrytis cinerea Botrytis cinerea are resistant to the judgement of UV-C abilities under different light medium
Botrytis cinerea is a kind of important necrotic type disease fungus of Ascomycetes, can infect 200 various plants, including height
The crops of value, such as:Strawberry, grape, Fresh Cutting flower etc. are to cause the important pathogen fungi of crops gray mold, and only secondary
In the second-biggest-in-the-world disease fungus of Pyricularia oryzae, huge economic loss can be caused to agricultural production.
Botrytis cinerea wild type used in this example is reference culture B05.10, washes lower botrytis cinerea spore, is carried out using spore
Botrytis cinerea is resistant to UV-C abilities and judges.By 70 L × 10 μ6It is flat that the spore suspension of/ml is uniformly coated on 1.5% water agar of solid
On plate and dry.Tablet is handled using UV-C, exposure intensity is respectively 20s, 40s, 60s, 80s, 100s, 120s.Own later
Sample be respectively put into immediately white light, blue light, feux rouges and it is dark lower cultivate 2h, after all samples are put under conditions of complete darkness
25±1℃10h.Microscope photographing sprouting situation, and count germination rate and rudiment length of tube.It is soft using ImageJ to sprout length of tube
Part measures.UV-C processing procedures are strictly protected from light, and whens different photo-irradiation treatments strictly avoids light from intersecting.The results are shown in Figure 4.
It can be seen from the results that with the growth of UV-C irradiation times, botrytis cinerea spore germination rate is gradually reduced;At UV-C
Irradiate 2h feux rouges after reason, botrytis cinerea spore germination rate and spore germination length of tube be substantially less than after UV-C processing irradiation 2h white lights and
Blue light, and germination rate under feux rouges and germ tube be compared under dark, no significant difference.
Embodiment 2, mould Penicillium are resistant to the judgement of UV-C abilities under different light medium
Mould is the postharvest pathogenic fungi for causing fruits and vegetables penicilliosis, and host is also very extensive, and causes citrus water
Postharvest pathogenic fungi the most serious is infected during being stored after fruit apple, peach, apricot picking.According to statistics, have sick as caused by mould
Evil loss caused by transporting procedures generally 10% or so, high is more than 30%, causes serious economic loss.
Wild-type strain used in this example is the one plant of mould detached from citrus, and it is resistance to equally to carry out its using mould spore
Judged by UV-C abilities.Specific method is consistent with example 1, and the results are shown in Figure 5.
It can be seen from the results that with the growth of UV-C irradiation times, Penicillium notatum spore germination rate is gradually reduced at UV-C
2h feux rouges is irradiated after reason, mould spore germination rate and sprouting length of tube are substantially less than irradiation 2h white lights and blue light after UV-C processing,
And under feux rouges germination rate and length of tube is sprouted with dark lower without significant difference, it presents equally to become with botrytis cinerea tolerance UV-C abilities
Gesture.
Embodiment 3, colletotrichum gloeosporioides Penz Colletotrichum gloeosporioides are resistant to UV-C under different light medium
Ability judges
Colletotrichum gloeosporioides Penz is the postharvest pathogenic fungi of a kind of global distribution, especially in the torrid zone of high temperature and humidity and Asia heat
Band can cause the postharvest disease of the various plants such as fruit tree, crops.Citrus is the largest fruit in the world, easily by true after adopting
Bacterium encroaches on, and causes largely to rot, leads to huge economic loss.And anthracnose is citrus fungi important in storage
One of disease.Not only host is extensive for colletotrichum gloeosporioides Penz, infect strategy it is also more various, so comparing at present for the prevention of the bacterium
It is difficult.
Wild-type strain used in this example is the one plant of colletotrichum gloeosporioides Penz detached from green lemon, equally uses colletotrichum gloeosporioides Penz
Spore carries out it and is resistant to the judgement of UV-C abilities.Specific method is consistent with example 1, and the results are shown in Figure 6.
It can be seen from the results that with the growth of UV-C irradiation times, colletotrichum gloeosporioides Penz germination rate is gradually reduced at UV-C
2h feux rouges is irradiated after reason, the germination rate and sprouting length of tube of colletotrichum gloeosporioides Penz are substantially less than irradiation 2h white lights and indigo plant after UV-C processing
Light, and under feux rouges germination rate and sprout length of tube with it is dark under without significant difference.
Causative effect compares after embodiment 4, UV-C processing infect the botrytis cinerea of grape.
Grape is most most wide one of a kind of fruit of ancient distribution in the world, almost account for whole world fruits output four/
One.Grape has very high nutritive value because of bright-colored, delicious flavour.Botrytis cinerea be wide host to adopt rear cause of disease true
Bacterium can infect 200 various plants, cause the gray mold of plant.Grape is the important host of botrytis cinerea, and grape grey mould is current
A kind of more serious disease occurs in the world, in the disease that all storages occur, the loss caused by it is the most serious.Cause
This prevents botrytis cinerea on grape and is of great significance.
Botrytis cinerea wild type used in this example is reference culture B05.10, and grape is to be bought from supermarket.This example be by
Botrytis cinerea spore carries out botrytis cinerea tolerance UV-C causative effects analysis using spore.Spore is suspended from PDB culture solutions, is added dropwise
10μL 5×105The spore suspension of/ml is under the grape surface for stabbing 5mm, different light medium after illumination 4h, 180s UV-C processing
Afterwards, grape is put back under the conditions of original different light quality after illumination 2h, all 25 ± 1 DEG C of 48h of dark.Photograph to record its feelings of causing a disease
Condition, and count its Lesion size.UV-C processing procedures are strictly protected from light, and whens different photo-irradiation treatments strictly avoids light from intersecting.
It can be seen from the results that after UV-C is handled, pathogenic significant decrease of the botrytis cinerea on grape, and feux rouges
With it is dark treated that the pathogenic processing for being substantially less than blue light and white light is pathogenic, therefore method of the present invention can
Disease applied to prevention fruits and vegetables.
In conclusion the present invention by botrytis cinerea, mould, three kinds of colletotrichum gloeosporioides Penz it is representative to adopt rear cause of disease true
Bacterium is resistant to UV-C abilities, it is found that UV-C abilities are resistant under the universal law feux rouges of postharvest pathogenic fungi to be remarkably decreased, and be inoculated in
Its feasibility is demonstrated on grape, having invented not only can effectively kill disease fungus but also the showcase of energy visual observation fruits and vegetables,
It can be used in real life.
The protection content of the present invention is not limited to above example.Without departing from the spirit and scope of the invention, originally
Field technology personnel it is conceivable that variation and advantage be all included in the present invention, and with appended claims be protect
Protect range.
Claims (8)
1. a kind of preserving fruit and vegetable utilizing showcase that can enhance short wavelength UV UV-C bactericidal effects, which is characterized in that including show area and
Compressor room;Wherein,
The show area includes glass cabinet door, UV-C ultraviolet germicidals, supporter, UV-C ultraviolet germicidals fixing groove and supporter
Fixing groove;The UV-C ultraviolet germicidals fixing groove is arranged on the supporter;The UV-C ultraviolet germicidals hang on institute
It states in UV-C ultraviolet germicidal fixing grooves;
The compressor room includes refrigeration system, temperature regulating device and distribution box.
2. the preserving fruit and vegetable utilizing showcase according to claim 1 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the glass cabinet door only selectively penetrates red visible.
3. the preserving fruit and vegetable utilizing showcase according to claim 1 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the supporter is engraved structure.
4. the preserving fruit and vegetable utilizing showcase according to claim 1 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In being respectively provided in every layer of UV-C ultraviolet germicidals fixing groove there are one UV-C ultraviolet germicidals.
5. the preserving fruit and vegetable utilizing showcase according to claim 1 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the UV-C ultraviolet germicidals are 15W.
6. the preserving fruit and vegetable utilizing showcase according to claim 1 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the refrigeration system includes:Compressor, condenser and evaporator and fan, the evaporator are located at below the fan.
7. the preserving fruit and vegetable utilizing showcase according to claim 5 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the temperature regulating device in the compressor realizes that temperature is adjusted with compressor linkage, can adjust in-cabinet temperature as needed
It is 0~16 DEG C.
8. the preserving fruit and vegetable utilizing showcase according to claim 7 that can enhance short wavelength UV UV-C bactericidal effects, feature exist
In the operation display area of the temperature control device includes temperature adjustment display screen and thermostat button, and the display screen and thermostat button are located at
The outer surface of the compressor room.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115462380A (en) * | 2022-11-01 | 2022-12-13 | 中国农业科学院植物保护研究所 | Compound preparation containing wuyiencin and application thereof in preventing and treating grape gray mold |
Citations (3)
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CN2320250Y (en) * | 1997-12-24 | 1999-05-26 | 吴子华 | Ultraviolet sterilization deodorizing device for refrigerator |
CN1423105A (en) * | 2001-12-03 | 2003-06-11 | 杜宪勇 | Vacuum fresh-keeping ultraviolet sterilizing electric refrigerator |
CN201668179U (en) * | 2009-10-23 | 2010-12-15 | 王龙岩 | Novel refrigerated display cabinet |
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CN115462380A (en) * | 2022-11-01 | 2022-12-13 | 中国农业科学院植物保护研究所 | Compound preparation containing wuyiencin and application thereof in preventing and treating grape gray mold |
CN115462380B (en) * | 2022-11-01 | 2023-02-03 | 中国农业科学院植物保护研究所 | Compound preparation containing wuyiencin and application thereof in preventing and treating grape gray mold |
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Application publication date: 20180724 |