CN108308403A - A method of correction feedstuff amino acid digestibility is prepared for pannage - Google Patents
A method of correction feedstuff amino acid digestibility is prepared for pannage Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of correction feedstuff amino acid digestibilities to be used for the method that pannage is prepared, including:(1) fistula is installed to collect the terminal ileum chyme of animal in grower pigs terminal ileum, measures the ileal digestibility of amino acid;(2) AID and SID when grower pigs half homozygous diet of feeding are measured under the conditions of controlled feeding;Daily ration amino acid digestibility when grower pigs feeding routine diet is measured under the conditions of free choice feeding;(3) the correction coefficient binary regression predictive equation of each amino acid digestibility is established with nutritional ingredient concentration in feed intake FI and diet:Equation is brought by the way that daily ingestion amount when the concentration of the amino acid in half homozygous daily ration and experiment is accounted for experiment pig percent weight, and the digestibility correction coefficient obtained is multiplied by the amino acid digestibility that half homozygous daily ration measures and obtains correction digestibility;It is verified, perfect compound feed is prepared using feedstuff amino acid digestion rate score after correction, gained mixed feed digestible aminoacids content is coincide substantially with actual measured value.
Description
Technical field
The present invention relates to a kind of methods that correction feedstuff amino acid digestibility is used for grower pigs feed formulation, belong to feeding
Expect technical field.
Background technology
It is accurate to evaluate feedstuff amino acid digestibility and be in the primary study of pig amino acid nutrient for feed formulation
Hold.It is in progress according to feedstuff amino acid Evaluation Research, terminal ileum amino acid digestibility can preferably represent pig to feed
Effective absorbing state of material protein nutrition, therefore half estimates growing-finishing pig using terminal ileum amino acid digestibility at present
Daily ration digestible aminoacids nutritional ingredient.Meanwhile the amino acid requirement of growing-finishing pig can also disappear usually using terminal ileum
Change nutrient to indicate.According to for pig Nitrogen Excretion situation bearing calibration difference, terminal ileum amino acid digestibility can be divided into
Apparent (AID) and standard (SID) ileal amino acid digestibility.
The evaluation of grower pigs feedstuff amino acid digestibility horizontal, Dietary Type and feeding by the CP in feed
The influence of amount.When carrying out digestion and metabolism measuring grower pigs feedstuff amino acid digestibility at present, grower pigs are usually fed
The half homozygous diet (feedstuff to be measured is diet sole protein matter source) based on cornstarch, daily ingestion amount are controlled in body
4% or so (controlled feeding) of weight.And the commercial feed fed during pig production is typically normal based on corn-soybean meal
Advise diet, and in the case of free choice feeding average daily gain up to grower pigs weight 5%.Existed using half homozygous diet
Amino acid digestibility is measured in the case of 4% feed intake for preparing the conventional diet based on corn in the case of free choice feeding
Feeding grower pigs certainly exist different.Therefore, the amino measured under the conditions of feeding is being limited by half homozygous daily ration in actual use
When sour digestibility prepares full price ration formulation, it is possible that estimating that digestible aminoacids content and actual measured value are not in daily ration
The phenomenon that symbol.In order to overcome the problems, such as this, the present invention is a kind of for correcting grower pigs feedstuff amino acid digestion by establishing
The method of rate so that the feed of preparation is more nearly the amino acid requirement of grower pigs.
Invention content
It is excellent it is an object of the invention to solve at least the above and/or defect, and provide at least to will be described later
Point.
In order to realize these purposes and other advantages according to the present invention, provides a kind of correction feedstuff amino acid and disappear
Rate is used for the method that pannage is prepared, and includes the following steps:
Step 1: fistula is installed in grower pigs terminal ileum by surgical operation, continuously to collect the terminal ileum of animal
Chyme, to measure the ileal digestibility of amino acid;
Step 2: under the conditions of controlled feeding, with half homozygous Diet test pig, different aminoacids water is measured respectively
Under flat, different feed intake levels, the conventional diet of corn, dregs of beans, wheat wheat-middlings, corn alcohol trough and its soluble matter and rapeseed dregs etc.
The apparent ileal amino acid digestibility and standard ileal amino acid digestibility of raw material;Wherein, day searches for food under the conditions of controlled feeding
Amount is calculated by the 3-5% of pig weight, is searched for food close to the 5% of pig weight day under the conditions of free choice feeding;
Step 3: establishing using nutritional ingredient concentration in diet and feed intake FI as each amino acid digestibility of independent variable
Correction coefficient binary regression predictive equation:
Arginine Arg:Y=1.2824-0.3732Arg-0.0574FI+0.0749Arg × FI, R2=0.9629, wherein
Arg concentration use scopes:0.33-0.12%;
Histidine:Y=1.3840-1.2096His-0.0745FI+0.2320His × FI, R2=0.9187, wherein
His concentration use scopes:0.20-0.53%;
Isoleucine Ile:Y=1.3968-0.7221Ile-0.0765FI+0.1367Ile × FI, R2=0.9012,
Middle Ile concentration use scope:0.30-0.75%;
Leucine Leu:Y=1.4631-0.4493Leu-0.0890FI+0.0851Leu × FI, R2=0.8951, wherein
Leu concentration use scopes:0.69-1.50%;
Lysine Lys:Y=1.4648-0.6348Lys-0.0942FI+0.1260Lys × FI, R2=0.9044, wherein
Lys concentration use scopes:0.44-1.15%;
Methionine Met:Y=1.3595-2.4545Met-0.0709FI+0.4734Met × FI, R2=0.8634, wherein
Met concentration use scopes:0.09-0.29%;
Phenylalanine Phe:Y=1.3499-0.5118Phe-0.0683FI+0.0982Phe × FI, R2=0.9107,
Middle Phe concentration use scope:0.40-1.00%;
Threonine Thr:Y=2.0128-2.0972Thr-0.2018FI+0.4158Thr × FI, R2=0.9920, wherein
Thr concentration use scopes:0.25-0.66%;
Tryptophan Trp:Y=1.5159-3.4109Trp-0.0996FI+0.6518Trp × FI, R2=0.9247, wherein
Trp concentration use scopes:0.08-0.24%;
Valine Val:Y=1.5190-0.8046Val-0.1019FI+0.1557Val × FI, R2=0.9345, wherein
Val concentration use scopes:0.36-0.95%;
Cystine Cys:Y=1.7016-4.5423Cys-0.1400FI+0.8839Cys × FI, R2=0.8668, wherein
Cys concentration use scopes:0.10-0.31%;
Wherein, feed intake FI is that daily dry matter intake accounts for experiment pig percentage of body weight, takes 3-5%;Nutrition in diet
Constituent concentration is dry matter percentage of each amino acid in diet, the i.e. concentration of the amino acid;By inciting somebody to action half homozygous daily ration
In the amino acid concentration and daily ingestion amount accounts for experiment pig percent weight and brings equation into when experiment, use the digestibility obtained
Correction coefficient is multiplied by the amino acid digestibility that half homozygous daily ration measures and obtains correction digestibility;
Step 4: measure grower pigs feeds Corn-soybean, corn-DDGS feeds, corn-dish under the conditions of free choice feeding
Daily ration standard ileal amino acid digestibility when the seed dregs of rice and corn-wheat wheat-middlings routine diet, verification establish mathematics through step 3
Whether the amino acid digestibility calculated value after model correction coincide with measured value;
Preferably, in the step 3, binary regression predictive equation uses the PROC REG sentences in SAS softwares to carry out
Model foundation.
The present invention includes at least following advantageous effect:The present invention passes through with the feed intake FI and diet under the conditions of controlled feeding
Middle nutritional ingredient concentration establishes the correction coefficient binary regression predictive equation of each amino acid digestibility, pre- by the binary regression
The correction coefficient that survey equation obtains is multiplied by the amino acid digestibility that half homozygous daily ration measures and obtains correction digestibility;Experience
Card prepares perfect compound feed using feedstuff amino acid digestion rate score after correction, and calculating gained mixed feed can digest
Amino acid content coincide substantially with actual measured value, and accuracy is better than using common half homozygous day grain method institute of academic circles at present
Measure feedstuff amino acid digestibility.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific implementation mode:
With reference to embodiment, the present invention is described in further detail, to enable those skilled in the art with reference to specification
Word can be implemented according to this.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other elements or combinations thereof.
Embodiment 1:
Feedstuff amino acid digestibility is measured in order to correct to press in experimental condition, to more accurately express the original
Expect the digestible aminoacids content in the mixed feed of practical pig production, it would be desirable to the feed measured under experimental condition
Raw material amino acid digestibility is corrected;
A method of correction feedstuff amino acid digestibility is prepared for pannage, is included the following steps:
Step 1: fistula is installed in grower pigs terminal ileum by surgical operation, continuously to collect the terminal ileum of animal
Chyme, to measure the ileal digestibility of amino acid;
Step 2: under the conditions of controlled feeding, with half homozygous Diet test pig, different aminoacids water is measured respectively
Under flat, different feed intake levels, the conventional diet of corn, dregs of beans, wheat wheat-middlings, corn alcohol trough and its soluble matter and rapeseed dregs etc.
The apparent ileal amino acid digestibility and standard ileal amino acid digestibility of raw material;Wherein, day searches for food under the conditions of controlled feeding
Amount is calculated by the 3-5% of pig weight, is searched for food close to the 5% of pig weight day under the conditions of free choice feeding;
Step 3: corn, dregs of beans, wheat wheat-middlings, corn alcohol trough and its soluble matter and vegetable seed that are measured according to step 2
The SID of different aminoacids, founding mathematical models correct feedstuff different aminoacids digestibility in the routine diet raw material such as dregs of rice
Measured value, so that the summation being multiplied by according to different feeds raw material amino acid digestibility corrected value after correction coefficient is matched equal to it
Make the amino acid digestibility of corresponding daily ration;Show gal4 amino acid digestibility in feed according to lot of experiments of the present invention
It is negatively correlated with feed intake under normal daily ration amino acid concentration, and be proportionate with feed intake when amino acid concentration is relatively low;
According to test data of the present invention, using the PROC REG sentences in SAS softwares, with feed intake, (FI, daily dry matter intake account for
Experiment pig percentage of body weight, 3-5%), nutritional ingredient concentration (dry matter percentage of each amino acid in daily ration) in daily ration,
Establish correction coefficient (long growing and fattening pigs are freely eaten, meet the daily ration of its nutritional requirements) binary of each amino acid digestibility
Regression prediction equation and amino acid concentration use scope are as follows:
Wherein, the correction coefficient calculating process of lysine digestibility is illustrated:
Using half homozygous daily ration of the dregs of beans containing lysine 1%, it is horizontal to be equivalent to weight 3%, 4% and 5% in daily ingestion amount
Lower measurement lysine SID is 85.1%, 85.0% and 79.5%;Using the homozygous daily ration of the dregs of beans containing lysine 0.5% half,
It is 76.1%, 80.3% and 80.7% that daily ingestion amount, which is equivalent to the horizontal lower lysine SID that measures of weight 3%, 4% and 5%,;Root
It observes according to the experiment, daily ingestion amount is about the 5% of weight when test pig is freely eaten, therefore uses 5% feed intake data to make herein
For the reference data in the case of free choice feeding, obtain in Dietary Lysine concentration 1%, lysine under three feed intake levels
SID is respectively 93.4%, 93.5% and 100% under the conditions of being freely eaten;Similarly, it is 0.5% in Dietary Lysine level
When, lysine SID is respectively 106.0%, 100.5% and 100% under the conditions of being freely eaten under three feed intake levels;If
Measure lysine SID be equivalent to be freely eaten under the conditions of the percentage of lysine SID be y, the half homozygous daily ration that when measurement uses
Lysine content is X1 (Lys), and the percentage that feed intake is equivalent to weight is X2 (FI), after great number tested data accumulation, root
It can show that the correction coefficient suitable for lysine is according to the regression analysis of SAS softwares:
Lysine Lys:Y=1.4648-0.6348Lys-0.0942FI+0.1260Lys × FI, R2=0.9044, wherein
Lys concentration use scopes:0.44-1.15%
Similarly, it is as follows that various essential amino acid correction coefficient are established:
Arginine Arg:Y=1.2824-0.3732Arg-0.0574FI+0.0749Arg × FI, R2=0.9629, wherein
Arg concentration use scopes:0.33-0.12%;
Histidine:Y=1.3840-1.2096His-0.0745FI+0.2320His × FI, R2=0.9187, wherein
His concentration use scopes:0.20-0.53%;
Isoleucine Ile:Y=1.3968-0.7221Ile-0.0765FI+0.1367Ile × FI, R2=0.9012,
Middle Ile concentration use scope:0.30-0.75%;
Leucine Leu:Y=1.4631-0.4493Leu-0.0890FI+0.0851Leu × FI, R2=0.8951, wherein
Leu concentration use scopes:0.69-1.50%;
Methionine Met:Y=1.3595-2.4545Met-0.0709FI+0.4734Met × FI, R2=0.8634, wherein
Met concentration use scopes:0.09-0.29%;
Phenylalanine Phe:Y=1.3499-0.5118Phe-0.0683FI+0.0982Phe × FI, R2=0.9107,
Middle Phe concentration use scope:0.40-1.00%;
Threonine Thr:Y=2.0128-2.0972Thr-0.2018FI+0.4158Thr × FI, R2=0.9920, wherein
Thr concentration use scopes:0.25-0.66%;
Tryptophan Trp:Y=1.5159-3.4109Trp-0.0996FI+0.6518Trp × FI, R2=0.9247, wherein
Trp concentration use scopes:0.08-0.24%;
Valine Val:Y=1.5190-0.8046Val-0.1019FI+0.1557Val × FI, R2=0.9345, wherein
Val concentration use scopes:0.36-0.95%;
Cystine Cys:Y=1.7016-4.5423Cys-0.1400FI+0.8839Cys × FI, R2=0.8668, wherein
Cys concentration use scopes:0.10-0.31%;
Wherein, including ten kinds must amino acid and a kind of nonessential amino acid cystine;Since pig is with centainly in body
The ability of interior synthesis nonessential amino acid, it is larger to be affected by this variation for the measurement of nonessential amino acid digestibility in feed;It returns
The foundation of equation has certain difficulty and precision is not high;But it also just can voluntarily be closed in pig body due to nonessential amino acid
At, in production It is not necessary to careful consideration daily ration in various non-essential amino acid contents, therefore it is within the rule go out;Cystine is in reality
Usually to consider jointly with methionine in growing-finishing pigs diets process for preparation, therefore regression prediction equation listed here.
It, need to be by the concentration of the amino acid and examination in half homozygous daily ration when correcting amino acid digestibility using regression equation
Daily ingestion amount accounts for experiment pig percent weight and brings equation into when testing, and half homozygous daily ration is multiplied by using the digestibility correction coefficient obtained
The amino acid digestibility measured obtains correction digestibility, can full price day that more accurately the estimated service life feedstuff is prepared
The digestible aminoacids content that grain is freely eaten in pig;
For example, it is 87% to measure dregs of beans lysine SID using half homozygous daily ration, lysine is dry in half homozygous daily ration when measurement
Content of material is 0.88%, and daily ingestion amount accounts for experiment pig weight 3.5%, substitutes into formula:
Y=1.4648-0.6348Lys-0.0942FI+0.1260Lys × FI,
Lys=0.88, FI=3.5 are wherein taken, is obtained:
Y=1.4648-0.6348 × 0.88-0.0942 × 3.5+0.1260 × 0.88 × 3.5=0.9646, derived from by adopting
It is 87% × 0.9646=83.9% to eat dregs of beans lysine digestibility after being corrected in mixed feed;
Another example, it is 68% to measure wheat wheat-middlings methionine digestibility using half homozygous daily ration, methionine in half homozygous daily ration
Content is 0.10%, and daily ingestion amount accounts for experiment pig weight 3.2%, substitutes into formula:
Y=1.3595-2.4545Met-0.0709FI+0.4734Met × FI,
Wherein Met=0.10, FI=3.2 are obtained:
Y=1.3595-2.4545 × 0.10-0.0709 × 3.2+0.4734 × 0.10 × 3.2=1.0387, derived from by adopting
It is 68% × 1.0387=70.6% to eat wheat wheat-middlings methionine digestibility after being corrected in mixed feed;
Step 4: measure grower pigs feeds Corn-soybean, corn-DDGS feeds, corn-dish under the conditions of free choice feeding
Daily ration standard ileal amino acid digestibility when the seed dregs of rice and corn-wheat wheat-middlings routine diet, verification establish mathematics through step 3
Whether the amino acid digestibility calculated value after model correction coincide with measured value;It is verified, use feedstuff amino after correction
Acid digestion rate score prepares perfect compound feed, calculates gained mixed feed digestible aminoacids content and actual measured value is basic
It coincide, accuracy is better than using feedstuff amino acid digestibility obtained by half homozygous daily ration.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and example shown and described herein.
Claims (2)
1. a kind of correction feedstuff amino acid digestibility is used for the method that pannage is prepared, which is characterized in that including following step
Suddenly:
Step 1: fistula is installed in grower pigs terminal ileum by surgical operation, continuously to collect the terminal ileum chyme of animal,
To measure the ileal digestibility of amino acid;
Step 2: under the conditions of controlled feeding, with half homozygous Diet test pig, it is horizontal, no that different aminoacids are measured respectively
With under feed intake level, the conventional diet raw material of corn, dregs of beans, wheat wheat-middlings, corn alcohol trough and its soluble matter and rapeseed dregs etc.
Apparent ileal amino acid digestibility and standard ileal amino acid digestibility;Wherein, under the conditions of controlled feeding daily ingestion amount by
The 3-5% of pig weight is calculated, and searches for food close to the 5% of pig weight day under the conditions of free choice feeding;
Step 3: establishing using nutritional ingredient concentration in diet and feed intake FI as the correction of each amino acid digestibility of independent variable
Coefficient binary regression predictive equation:
Arginine Arg:Y=1.2824-0.3732Arg-0.0574FI+0.0749Arg × FI, R2=0.9629, wherein Arg is dense
Spend use scope:0.33-0.12%;
Histidine:Y=1.3840-1.2096His-0.0745FI+0.2320His × FI, R2=0.9187, wherein His is dense
Spend use scope:0.20-0.53%;
Isoleucine Ile:Y=1.3968-0.7221Ile-0.0765FI+0.1367Ile × FI, R2=0.9012, wherein Ile
Concentration use scope:0.30-0.75%;
Leucine Leu:Y=1.4631-0.4493Leu-0.0890FI+0.0851Leu × FI, R2=0.8951, wherein Leu is dense
Spend use scope:0.69-1.50%;
Lysine Lys:Y=1.4648-0.6348Lys-0.0942FI+0.1260Lys × FI, R2=0.9044, wherein Lys is dense
Spend use scope:0.44-1.15%;
Methionine Met:Y=1.3595-2.4545Met-0.0709FI+0.4734Met × FI, R2=0.8634, wherein Met is dense
Spend use scope:0.09-0.29%;
Phenylalanine Phe:Y=1.3499-0.5118Phe-0.0683FI+0.0982Phe × FI, R2=0.9107, wherein Phe
Concentration use scope:0.40-1.00%;
Threonine Thr:Y=2.0128-2.0972Thr-0.2018FI+0.4158Thr × FI, R2=0.9920, wherein Thr is dense
Spend use scope:0.25-0.66%;
Tryptophan Trp:Y=1.5159-3.4109Trp-0.0996FI+0.6518Trp × FI, R2=0.9247, wherein Trp is dense
Spend use scope:0.08-0.24%;
Valine Val:Y=1.5190-0.8046Val-0.1019FI+0.1557Val × FI, R2=0.9345, wherein Val is dense
Spend use scope:0.36-0.95%;
Cystine Cys:Y=1.7016-4.5423Cys-0.1400FI+0.8839Cys × FI, R2=0.8668, wherein Cys is dense
Spend use scope:0.10-0.31%;
Wherein, feed intake FI is that daily dry matter intake accounts for experiment pig percentage of body weight, takes 3-5%;Nutritional ingredient in diet
Dry matter percentage of a concentration of each amino acid in diet, the i.e. concentration of the amino acid;By that will be somebody's turn to do in half homozygous daily ration
Daily ingestion amount accounts for experiment pig percent weight and brings equation into when the concentration of amino acid and experiment, is corrected using the digestibility obtained
Coefficient is multiplied by the amino acid digestibility that half homozygous daily ration measures and obtains correction digestibility;
Step 4: measure grower pigs feeds Corn-soybean, corn-DDGS feeds, corn-rapeseed dregs under the conditions of free choice feeding
With daily ration standard ileal amino acid digestibility when corn-wheat wheat-middlings routine diet, verify through step 3 founding mathematical models
Whether the amino acid digestibility calculated value after correction coincide with measured value.
2. correction feedstuff amino acid digestibility is used for the method that pannage is prepared as described in claim 1, feature exists
In in the step 3, binary regression predictive equation uses the PROCREG sentences in SAS softwares to carry out model foundation.
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CN114886010A (en) * | 2021-04-29 | 2022-08-12 | 西南科技大学 | Preparation method of microecological preparation for pigs |
CN115444078A (en) * | 2022-08-31 | 2022-12-09 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Amino acid balance method of non-grain plant protein based on enzymolysis hydration reaction |
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CN114886010A (en) * | 2021-04-29 | 2022-08-12 | 西南科技大学 | Preparation method of microecological preparation for pigs |
CN114886010B (en) * | 2021-04-29 | 2023-05-23 | 西南科技大学 | Preparation method of microecological preparation for pigs |
CN114223795A (en) * | 2021-12-21 | 2022-03-25 | 西南科技大学 | Preparation method of probiotic feed for piglets |
CN114223795B (en) * | 2021-12-21 | 2023-05-23 | 西南科技大学 | Preparation method of probiotics feed for piglets |
CN114742290A (en) * | 2022-03-30 | 2022-07-12 | 东北农业大学 | Method for predicting conversion efficiency of white feather broiler feed through plasma metabolite abundance modeling |
CN115444078A (en) * | 2022-08-31 | 2022-12-09 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Amino acid balance method of non-grain plant protein based on enzymolysis hydration reaction |
CN115444078B (en) * | 2022-08-31 | 2024-05-31 | 山东省海洋资源与环境研究院(山东省海洋环境监测中心、山东省水产品质量检验中心) | Amino acid balance method of non-grain vegetable protein based on enzymolysis hydration reaction |
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