CN108293461A - Magnolia wufengensis engrafting method - Google Patents
Magnolia wufengensis engrafting method Download PDFInfo
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Abstract
The present invention proposes the engrafting method of Magnolia wufengensis.This method includes that scion is grafted onto on stock, and stock includes being selected from one of Magnolia wufengensis and Y biondii(Pamp)D.L.Fu, wherein the scion is red No. 1 tender.Red No. 1 nursery stock production of spoiling on a large scale that economic, grafting survival rate is high and increment is big can be realized using the engrafting method.
Description
Technical field
The present invention relates to plant fields, in particular it relates to Magnolia wufengensis engrafting method.
Background technology
Magnolia wufengensis (Magnolia wufengensis L.Y Ma et L.R.Wang) is that Magnoliaceae Magnolia yulan is sub-
Belong to novel species, was taught by Beijing Forestry University Ma Lvyi in 2006 and Wang Luorong engineer is in Hubei Wu Feng height above sea level 1400~2000
It is found at rice, which is deciduous tree, first spends rear leaf, Hua great, petal quantity 9~46, pattern has dark red, red and pale red.It is existing
Yulan be white, pale red and purple, and be red yulan inside and outside flower piece be to find for the first time.The discovery of Magnolia wufengensis,
Not only be Magnolia phylogeny lay a good foundation, but also have medicinal and ornamental value, make it have greatly production and
Promotional value.Tender red No. 1 is the new varieties selected from Magnolia wufengensis, and tapel 9 is close equal, is inside and outside entire tapel
Uniform peony is pure red new varieties inside and outside first in China's Magnoliacea plant, and the speed of growth is also significantly better than commonly
Yulan kind has great ornamental value.The good characteristic and reproduction speed that vegetative propagation has the characteristics that keep maternal are fast,
In the reproductive process of new varieties, a large amount of choiceness single plant can be quickly obtained by grafting and with the period is short, opens
Spend early advantage.
However, red No. 1 graft technology of spoiling still needs the further research and exploration of forest-science man.
Invention content
The application is made to the discovery of following facts and problem and understanding based on inventor：
In the natural environment, existing more than 2000 strain of Magnolia wufengensis, and natural regeneration almost without, therefore protect Magnolia wufengensis
It is very urgent.Magnolia wufengensis has had begun grafting work in production, also achieves certain success, but existing graft technology
Success rate it is relatively low.Discovery based on the above issues, inventor develop establish it is a set of based on suitable stock type, stock
The red No. 1 new varieties grafting and breeding technical system of spoiling of seedling age, grafting method and grafting time, the system can realize economic, grafting
The extensive nursery stock production that survival rate is high and increment is big.Meanwhile inventor carries out also for Magnolia wufengensis graft union process
Graft union and seedling wound mouth relevant enzymes activity, soluble sugar content, Relative electro-conductivity in agglutination have been inquired into research
The variation of rate understands the Related Mechanism of the variations such as the inside enzyme during Magnolia wufengensis graft union, to judge graft union
Critical period, provide fundamental basis and practical advice to improve grafting shoot survival percent.
In the first aspect of the present invention, the present invention proposes a kind of engrafting method of Magnolia wufengensis.Implemented according to the present invention
Example, scion is grafted onto on stock, and the stock includes being selected from one of Magnolia wufengensis and Y biondii(Pamp)D.L.Fu, wherein institute
It is red No. 1 tender to state scion.It can realize that economical, grafting survival rate is high and grows using engrafting method according to the ... of the embodiment of the present invention
Big red No. 1 nursery stock production of spoiling on a large scale of amount.
According to an embodiment of the invention, above-mentioned engrafting method further comprises at least one following additional technical feature：
According to an embodiment of the invention, the stock is Y biondii(Pamp)D.L.Fu.Inventor has found that Y biondii(Pamp)D.L.Fu is relatively cheap, because
And it can realize more economical scale nursery stock production.
According to embodiments of the present invention, the stock seedling age is life in 2~4 years.Inventor find, using 2~4 years raw stocks into
Row grafting, high graft survival rate.
According to an embodiment of the invention, the stock seedling age is life in 4 years.Inventor has found, is carried out using 4 years raw stocks
Grafting, graft survival rate higher, red No. 1 growth of spoiling after grafting is more healthy and stronger, is more suitable for the nursery stock production of scale.
According to an embodiment of the invention, the time of the grafting is 1 day~September of August 1.Inventor had found, in August 1 day
~September is grafted on the 1st, and high graft survival rate, increment are big.
According to an embodiment of the invention, the time of the grafting is August 10~20 (mid-August).Inventor has found
In the scale nursery stock production that can more realize that high graft survival rate, increment are big that mid-August is grafted.
According to an embodiment of the invention, the time of the grafting is August 15.Inventor has found, is transferred within 15th in August
It connects, grafting success rate significantly improves.
According to an embodiment of the invention, the temperature of the grafting is 19~34 DEG C.Inventor has found, is carried out at 19~34 DEG C
Grafting, high graft survival rate, seedling growth amount are big.
According to an embodiment of the invention, the temperature range of the grafting time is 21~25 DEG C.Inventor has found, 21~
25 DEG C are grafted, and graft survival rate higher, seedling growth be more healthy and stronger.
According to embodiments of the present invention, the grafting is connect and is connect with I-shaped bud by simple bud side grafting, embedding bud, T font buds
At least one realize.Inventor has found, is grafted by the way of at least one above-mentioned, and grafting success rate is high.
According to an embodiment of the invention, the grafting is to connect realization by simple bud side grafting or T font buds.Inventor has found
High graft survival rate and the big scale nursery stock production of output can more be realized by being connect by simple bud side grafting and T font buds.
In the second aspect of the present invention, the present invention proposes a kind of engrafting method of Magnolia wufengensis.Implemented according to the present invention
Example, the method include：It is described tender red with tender red No. 1 for scion using 2~4 years raw Magnolia wufengensis or Y biondii(Pamp)D.L.Fu as stock
No. 1 branch for ripe elite stand current year life is connect mode and is grafted using simple bud side grafting or T font buds, the time of the grafting
Temperature for 1 day~September of August 1 day, the grafting is 19~34 DEG C.It should be noted that tender red No. 1 is ripe elite stand current year
" ripe elite stand " in raw branch refers to the elite stand of 3~5 years raw robust growths.Engrafting method according to the ... of the embodiment of the present invention
It can realize economy, graft survival rate higher and the big scale nursery stock production of increment.
Description of the drawings
The above-mentioned and/or additional aspect and advantage of the present invention will become in the description from combination following accompanying drawings to embodiment
Obviously and it is readily appreciated that, wherein：
Fig. 1 is the variation of soluble sugar during red No. 1 graft union of spoiling according to the embodiment of the present invention；
Fig. 2 is the variation of relative conductivity during red No. 1 graft union of spoiling according to the embodiment of the present invention；
Fig. 3 is the active variations of SOD during red No. 1 graft union of spoiling according to the embodiment of the present invention；
Fig. 4 is the active variations of CAT during red No. 1 graft union of spoiling according to the embodiment of the present invention；
Fig. 5 is the active variations of POD during red No. 1 graft union of spoiling according to the embodiment of the present invention；
Fig. 6 is the active variations of PAL during red No. 1 graft union of spoiling according to the embodiment of the present invention；And
Fig. 7 is the active variations of PPO during red No. 1 graft union of spoiling according to the embodiment of the present invention.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings.Below with reference to
The embodiment of attached drawing description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
In the examples below, data carry out data calculating using Microsoft Excel and draw a diagram, with R language
Carry out variance analysis, Multiple range test and correlation analysis.
Influence of the 1 stock seeds of embodiment to graft survival rate
In Augusts, 2015 just inventor respectively using 2 years identical Y biondii(Pamp)D.L.Fus of raw specification, Magnolia wufengensis seedlings as stock,
With full tender red No. 1 of robust growth, sprout for scion, grafted with the engrafting method of simple bud side grafting.Using random district's groups
Design remembers that 2 processing, each 30 plants of processing grafting are repeated 3 times, remember 180 plants altogether.Administrative skill measure phase is respectively handled after grafting
Together.Second year is sprouted since bud after grafting, and survival rate is counted after every 15d and records seedling growth situation.As shown in Table 1, with
Magnolia wufengensis and the survival rate that Y biondii(Pamp)D.L.Fu is stock are respectively 86.67%, 83.33%, the former survival rate is higher than the latter by 3.34
Percentage point；It is respectively 106.71cm, 81.44cm using Magnolia wufengensis and Y biondii(Pamp)D.L.Fu as the Seedling height of stock, the former Seedling height
Higher 25.27cm than the latter, the former exceeds the latter 31%.It is respectively as the girth growth of stock using Magnolia wufengensis and Y biondii(Pamp)D.L.Fu
16.65mm, 11.12mm, the former Seedling height is higher 5.53mm than the latter, the former exceeds the latter 49%.To its graft survival rate R languages
Speech carries out variance analysis, value=0.287 P>0.05, the results showed that, select Magnolia wufengensis and Y biondii(Pamp)D.L.Fu to be not significantly different.From
Economic angle sets out, and for Magnolia wufengensis as new species, value is high；Y biondii(Pamp)D.L.Fu is relatively cheap, preferably as stock seeds.
Influence of the different stock types of table 1 to graft survival rate and increment
| Stock type | Average graft survival rate/% | Average Seedling height/cm | Average girth growth/mm |
| Y biondii(Pamp)D.L.Fu | 83.33 | 81.44 | 11.12 |
| Magnolia wufengensis | 86.67 | 106.71 | 16.65 |
Influence of the 2 stock seedling age of embodiment to graft survival rate
Just inventor is full with robust growth, sprout using life in 2 years, 4 years raw Y biondii(Pamp)D.L.Fus as stock in August, 2015
Tender red No. 1 is scion, is grafted with the method for simple bud side grafting.Using RANDOMIZED BLOCK DESIGN, remember that 2 processing, each processing are transferred
30 plants are connect, is repeated 3 times, remembers 180 plants altogether.It is identical that administrative skill measure is respectively handled after grafting.Second year is sprouted since bud after grafting
It is dynamic, graft survival rate is counted after every 15d and records seedling growth situation.As shown in Table 2, it is with life in 4 years, 2 years raw Y biondii(Pamp)D.L.Fus
The graft survival rate of stock is respectively 86.67%, 73.33%, the former graft survival rate is 13.34 percentage points higher than the latter, preceding
Person exceeds the latter 18%.It is respectively 59.74cm, 75.63cm using life in 4 years, 2 years raw Y biondii(Pamp)D.L.Fus as the Seedling height of stock, the former
Seedling height is lower 15.89cm than the latter, the former is less than the latter 26%.Using life in 4 years, 2 years raw Y biondii(Pamp)D.L.Fus as the girth growth of stock point
Not Wei 11.85mm, 10.51mm, the former girth growth 1.34mm more than the latter, the former exceed the latter 12%.To its survival rate R languages
It is 0.00906 that speech, which carries out variance analysis P values,<0.01, the results showed that, life in 2 years, 4 years raw Y biondii(Pamp)D.L.Fus are made stock and are existed significantly
Difference, the results showed that：Raw Y biondii(Pamp)D.L.Fu is as stock optimum within 4 years.Although 2 years raw stock Seedling heights were than 4 years, raw Seedling height is high
26 percentage points, but the growth of stock, girth growth are the first indexs, Seedling height is the second index, and the first indicator difference is apparent,
The effect of second index also accordingly reduces.Thus 4 years raw Y biondii(Pamp)D.L.Fus are as stock optimum.
The influence of influence and increment of the different stock seedling ages of table 2 to graft survival rate
| Stock type | Average graft survival rate/% | Average Seedling height/cm | Average girth growth/mm |
| Life in 2 years | 73.33 | 75.63 | 10.51 |
| Life in 4 years | 86.67 | 59.74 | 11.85 |
Influence of 3 grafting time of embodiment to graft survival rate
August in 2015 1 day, August 15 days, September inventor on the 1st are using 2 years identical Y biondii(Pamp)D.L.Fus of raw specification as stock, with life
Length is healthy and strong, sprout is full tender red No. 1 is scion, is grafted with the method for simple bud side grafting.August temperature range on the 1st is 21
~34 DEG C；August temperature range on the 15th is 21~28 DEG C；September temperature range on the 1st is 19~34 DEG C.3 processing of note, each
30 plants of processing grafting, is repeated 3 times, 270 plants totally.It is identical that administrative skill measure is respectively handled after grafting.Second year is opened from bud after grafting
Beginning sprouts, and graft survival rate is counted after every 15d and records seedling growth situation.As shown in Table 3, August 1 day, August 15 days, September 1
The graft survival rate of day is respectively 73.33%, 83.33%, 76.77%, and August graft survival rate on the 15th is respectively higher than August 1
With 10 percentage points and 6.56 percentage points of September 1 day, August graft survival rate on the 15th exceeds August 1 day and September 1 day respectively
13% and 9%.August 1 day, August 15 days, September average Seedling height on the 1st are respectively 75.63cm, 81.44cm, 59.09cm, August
Average Seedling height on the 15th is more than respectively August 1 and September 1 day 7%, 37%.August 1 day, August 15 days, September 1 day it is average thick
Growth is respectively 10.51mm, 11.12mm, 10.50mm, and August average girth growth on the 15th is more than August 1 day and September 1st is
5%.Variance analysis is carried out with R language to its survival rate, P values are 0.0263<0.05, the results showed that, August 1 day, August 15 days, 9
The moon, there are significant differences on 1st.To carry out Multiple range test with LSD, as shown in Table 4, August grafting time optimum on the 15th.
Influence of the different grafting times of table 3 to graft survival rate and increment
The Multiple range test of the different grafting times of table 4
| Grafting time | 5% level of signifiance | 1% level of signifiance |
| August 1 day | c | A |
| August 15 days | a | A |
| September 1 day | b | A |
Influence of 4 engrafting method of embodiment to graft survival rate
Just inventor is full with robust growth, sprout using 2 years identical Y biondii(Pamp)D.L.Fus of raw specification as stock in August, 2015
Tender red No. 1 be scion, grafted respectively in the method for simple bud side grafting, plate budding.2 processing of note, each processing grafting 30
Strain, is repeated 3 times, 180 plants totally.It is identical that administrative skill measure is respectively handled after grafting.Second year is sprouted since bud after grafting, often
Graft survival rate is counted after 15d and records seedling growth situation.As shown in Table 5, the graft survival rate point of simple bud side grafting, plate budding
It Wei 73.33%, 56.67%., than 16.66 percentage points of the high graft survival rate of the latter, the former exceeds the latter 29% for the former.It is single
Bud side grafting, plate budding average Seedling height be respectively 75.63cm, 70.59cm, the former is higher than the average Seedling height of the latter
5.04cm, the former exceeds the latter 7%；Simple bud side grafting, plate budding average girth growth be respectively 10.51mm, 8.35mm, the former compares
The high 2.16mm of average girth growth of the latter, the former exceeds the latter 25%.To its survival rate with R language carry out variance analysis P values=
0.0038<0.01, the results showed that：There are pole significant differences with plate budding for simple bud side grafting, it was demonstrated that simple bud side grafting is optimum transfers
Connect method.
The influence of influence and increment of 5 different grafting methods of table to graft survival rate
| Grafting method | Average graft survival rate % | Average ground diameter/cm | Average Seedling height/cm | Average girth growth/mm |
| Simple bud side grafting | 73.33 | 12.76 | 75.63 | 10.51 |
| Plate budding | 56.67 | 12.56 | 70.59 | 8.35 |
Influence of 5 engrafting method of embodiment to graft survival rate
In mid-August, 2016, inventor was using 2 years identical Y biondii(Pamp)D.L.Fus of raw specification as stock, full with robust growth, sprout
Full tender red No. 1 is scion, is connect respectively with simple bud side grafting, T-shaped bud, the method for I-budding is grafted.At note 3
Reason, each 30 plants of processing grafting, is repeated 3 times, 270 plants totally.50 days statistics survival rates after grafting.Administrative skill is respectively handled after grafting
Measure is identical.As shown in Table 6, simple bud side grafting, T font buds connect, I-shaped bud connects graft survival rate be 75.56%,
77.56%, the graft survival rate that 19.23%, T fonts bud connects connects 2 percentage points and 42.2 higher than simple bud side grafting and I-shaped bud
Percentage point, the graft survival rate that font bud connects connects 2% and 303% beyond simple bud side grafting and I-shaped bud.To its grafting survival
Rate carries out variance analysis P values=7.13e-12***<0.01, the results showed that：Simple bud side grafting connects with T font buds, I-shaped bud connects and deposits
In pole significant difference.As shown in Table 7, simple bud side grafting and T font buds connect, and are optimum engrafting methods.
The average graft survival rate of 6 different grafting methods of table
| Simple bud side grafting | T font buds connect | I-shaped bud connects | |
| Y biondii(Pamp)D.L.Fu | 75.56 | 77.56 | 19.23 |
The Multiple range test of 7 different grafting methods of table
| Engrafting method | 5% level of signifiance | The 1% pole level of signifiance |
| Simple bud side grafting | a | A |
| T font buds connect | a | A |
| I-shaped bud connects | b | B |
6 healing mechanism of embodiment is studied
About graft union process, the period of different researchers' divisions simultaneously differs.This experiment divides agglutination
Healing mechanism research is carried out for 5 stages.I.e.：Separation layer forms the phase, callus is differentiated to form the phase, callus connects the phase,
Forming layer is differentiated to form the phase, vascular bundle is differentiated to form the phase.
Using 2 years raw Y biondii(Pamp)D.L.Fus as stock, leaf of the stock apart from ground 50cm is all gone the inventor on the 15th of August in 2016
Fall, it is oblique longitudinal sectional from top to down at the position away from ground 15-20cm, up to xylem surface from epidermis to cortex, downwards
About 5cm is cut, then the bark of incision is cut about half.With tender red No. 1 of robust growth for scion, with the method for simple bud side grafting
It is grafted.The processing for compareing seedling is consistent with the processing of stock, totally 54 plants.0d, 4d after grafting, 8d, 12d, 18d, 24d,
30d, 36d, 42d are sampled 9 times altogether.It is sampled using completely random method, is repeated 3 times every time, sample 54 plants altogether.Acquisition grafting
The branch section of 5cm above and below position, is packed into valve bag, places in liquid nitrogen and takes back laboratory, is stored in subzero 80 DEG C of refrigerators.After 42 days
Sampling terminates unified measurement physical signs.
Soluble sugar content measures：
As a result it calculates：
C is the sugar content checked on standard curve, unit u/g；
V is extracting liquid volume, this experiment takes 50mL；
Vs is to take volume when measuring, and takes 1mL；
W is sample fresh weight, unit g；
As shown in Figure 1, the soluble sugar content of 0~20d graftings is average daily less than control significantly lower than control
0.53%, wherein 4d, 12d difference are particularly evident after grafting, respectively lower than compare 56%, 37%；20~42d graftings are soluble
Sugared content is average daily higher than control 0.74% higher than control.To healing latter stage, grafting soluble sugar content restores to initial
Level is suitable with control soluble sugar content.It is in " two peaks " to compare soluble sugar content its change curve in agglutination
There is peak in 12d, 36d respectively in feature, and content is the 101% and 97% of same time grafting soluble sugar content respectively.
And grafting soluble sugar content its change curve in agglutination is in the feature on " 3 peaks ", 3 peaks respectively appear in
8d, 24d and 36d after grafting, soluble sugar content are 98%, 201% and the 103% of same time control respectively.Meanwhile it grafting
Seedling soluble sugar content its change curve in agglutination is in the feature of " 3 low ebbs ", after 3 low ebbs respectively appear in grafting
4d, 18d and 3d, soluble sugar content are 44%, 76% and the 125% of same time control respectively.
There is low ebb in 4d, 18d and 3 0d in grafting soluble sugar content in the present embodiment, 4~8d, 18~24d, 30~
36d soluble sugar contents increase, and are to break up and provide energy for cell division.Inventor deduces：4d is callus differentiation
The formation phase；18d is the callus connection phase, and 30d is that forming layer is differentiated to form the phase.
As a result it calculates：
S1 is conductivity gauge preliminary survey electric conductivity value
S2 is to survey electric conductivity value eventually
As shown in Figure 2, in agglutination, 2~12d grafting relative conductivities are average every higher than control relative conductivity
It is higher by 0.06%；12~42d grafting relative conductivity levels are below control, average daily less than control 0.95%.It is right
According to relative conductivity in agglutination its change curve be in " two peaks " feature, in 18d, 30d there is peak, phase in differentiation
It is same time grafting soluble sugar content 107% and 112% respectively to conductivity.And grafting relative conductivity is cured in grafting
Its change curve is in the feature on " 3 peaks " during conjunction, and 3 peaks respectively appear in 4d, 18d and 30d after grafting, opposite
Conductivity is same time control soluble sugar content 137%, 93% and 89% respectively.When plant is by adverse circumstance, cell membrane by
Damage, membrane permeability increase, Electrolyte Leakage, cause the conductivity of plant cell to increase.Meanwhile separation layer forms phase and Vascular bridge
Be differentiated to form the stage, the two phase cell membrane permeabilities can improve accordingly.Inventor deduces：4d may be to be in isolation
Layer forms the phase, and 30d is to be differentiated to form the phase in vascular bundle.
Hepatocuprein (SOD) determination of activity：
As a result it calculates：
A is the reading for being divided range meter；
VT, which is measured, uses enzyme solution volume, this experiment to take 4mL；
V1 enzyme coarse extraction volumes, take 0.05mL；
W is sample fresh weight, unit g.
From the figure 3, it may be seen that the SOD activity of grafting and control seedling healing part is all in the trend fallen after rising.0 after grafting
~6d, grafting are averaged SOD activity less than control 2.2%；6~42d graftings are averaged SOD activity higher than control 3.3%.Healing
Part SOD activity is low, promotes callus to be formed, to improve graft survival rate.The SOD activity of 6~42d controls is all less than
Grafting illustrates that the healing ability of generally grafting is less than control.
There is peak in 4d, 12d in control SOD activity, is 108%, the 89% of same period grafting.And grafting SOD activity
Variation be in " bimodal " curve, since grafting the same day i.e. slowly rise, 12d reaches the first peak value, and activity value is than initial stage liter
It is high by 60.78%, it is higher than concurrent control by 11.29%；Then being declined, 18~24d gos up again, and second peak value occurs in 24d,
Activity ratio's concurrent control is high by 29.89%, and development latter stage gradually declines again.Wherein, first peak value is higher by second peak value
7%.In the period of 2 peak intervals：12~24d, grafting SOD average activities are 263.22U/g FWmin, compare seedling
The 229.16U/g FWmin of SOD average activities, grafting SOD activity are higher by 14.8% than control, show that this period can
It can be the critical period of graft union healing.
Catalase (CAT) determination of activity:
As a result it calculates：
A is the reading for being divided range meter；
VT, which is measured, uses enzyme solution volume, this experiment to take 4mL；
V1 enzyme coarse extraction volumes, take 0.05mL；
W is sample fresh weight, unit g；
T is to add hydrogen peroxide single reading time to the end.
As shown in Figure 4, grafting CAT activity is lower than compareing on the whole, only lives in 0~8d and 17~19d graftings CAT
Property higher than control, grafting be averaged CAT activity higher than compare 3.5%；9~17d, 19~42d are that grafting CAT activity is less than
Control, grafting are averaged CAT activity less than control 4.7%.There are 3 peaks in 4d, 18d, 30d in grafting CAT activity, respectively
It is 124%, 111%, the 86% of same time control.And compare CAT activity and 2 peaks occur in 12d, 30d, it is the same time respectively
171%, the 111% of grafting.
CAT is increased rapidly as Protective enzyme in plant when plant comes to harm, and protects the plants from injury, and separation layer
The effect of formation phase is to prevent moisture evaporation, and wound is protected not invaded by harmful substance, is the first rank during graft union
Section.There is peak higher than control, grafting in 4d CAT activity in 0~8d graftings CAT activity in the present embodiment；Inventor speculates
It is that separation layer forms the phase to go out 0~4d.
Peroxidase (POD) determination of activity:
As a result it calculates：
OD is the reading for being divided range meter；
VT, which is measured, uses enzyme solution volume, this experiment to take 4mL；
V1 enzyme coarse extraction volumes, take 0.3mL；
W is sample fresh weight, unit g；
As shown in Figure 5, only for 18d, 30d grafting less than control, remaining is all grafting higher than control, average grafting
POD activity is higher by 5.1% than control.Grafting POD activity goes out in the trend for subtracting be alternately present afterwards is first increased respectively at 24d, 36d
Existing peak, 24d POD activity reach 849.9U/g FWmin, and 36d POD activity reaches 800U/g FWmin, 24d,
36d POD activity is the 136% and 129% of its same time control respectively.And compare the active overall variations of POD and tend to be steady,
There is small peak in 18d, 30d, 18d reaches 650.1U/g FWmin, and the activity of 30d reaches 665.9U/g FWmin,
18d, 30d POD activity are the 108% of its same time grafting, 77.5% respectively.
POD is as protective enzyme, when plant comes to harm, will rise rapidly and protects the plants from injury, and separation layer
Effect is to prevent moisture evaporation, and wound is protected not invaded by harmful substance, and plant is exactly protected in the active raisings of 0~4d POD
It escapes injury, it is that separation layer forms the phase that can deduce 0~4d.Meanwhile POD is connected with callus during graft union
It closes.In the present embodiment, active first peak grafting POD comes across 24d after grafting, therefore can speculate：24d is callus
The connection phase.Correlative study thinks that the raising of POD activity contributes to vascular bundles characteristics and connection, and 36d POD activity occurs after grafting
Second peak, thus speculates：36d is the period for connecting the conducting tissue of anvil fringe and being differentiated to form (vascular bundle is differentiated to form the phase).
Phenylalanine lyase (PAL) determination of activity：
As a result it calculates：
OD is the reading for being divided range meter
V2, which is measured, uses enzyme solution volume, this experiment to take 0.5mL；
V1 enzyme coarse extraction volumes, take 4mL；
W is sample fresh weight, unit g；
It is fluctuated it will be appreciated from fig. 6 that grafting PAL activity and control are all in wave, is all first to reduce to increase again to be alternately present
Variation tendency.Between 0~42d, grafting PAL activity 24d after grafting reaches peak, minimum occurs in 4d, point
It is not active 143% and 38% when just grafting, peak is 3.77 times of minimum；And it is also to be reached in 24d to compare PAL activity
To peak, there is minimum in 4d, and active 120% and 65% when being rigid callus processing respectively, peak is minimum
1.85 again.The active peaks of grafting PAL are corresponding compare ratio 2.03 times to the ratio of minimum, so agglutination
The middle active variations of grafting PAL are fiercer than control variation.
There is peak in 12d and 24d in grafting PAL activity, and value is 72.93U/g FWmin, 71.77U/g respectively
The PAL activity of FWmin, 12d, 24d are the 184% and 118% of its same time control respectively.PAL activity is compareed in 8d, 24d
There is peak, respectively 40.09U/g FWmin, 60.50U/g FWmin are 57% He of its same time grafting respectively
84%.PAL is most important enzyme during secondary xylem differentiation, is thickened for secondary wall during xylem differentiation.This
When, PAL can promote the forming layer of anvil fringe to carry out vigorous differentiation activity, form a large amount of secondary xylem；It can also promote anvil
Separation layer is broken through in a large amount of division and proliferations of callus of the wooden xylem and scion junction, part, forms the callus of connection anvil fringe
Organize bridge.In agglutination, there is peak in grafting PAL activity 12d and 24d after grafting.Inventor deduces：
The forming layer of 12d anvil fringes carries out vigorous differentiation, and a large amount of division and proliferations of callus of 24d anvil fringes junction form callus
Organize bridge.To obtain：12d is that callus is differentiated to form the phase；24d is the callus connection phase.Meanwhile PAL has promotion
The effect of cell differentiation and lignifying, cell differentiation and lignifying period can be in rising trend.4~12d, 18~24d, 30~
3 periods of 42d are the grafting PAL active rising stages, and callus is differentiated to form phase, callus connection phase, vascular bundle
Being differentiated to form the PAL activity in 3 periods of phase can also increase.Thus speculate：4~12d is that callus is differentiated to form the phase；18~
24d is the callus connection phase；30~42d is that vascular bundle is differentiated to form the phase.
Polyphenol oxidase (PPO) determination of activity：
As a result it calculates：
OD is the reading for being divided range meter；
W is sample fresh weight, unit g；
Above physical signs is all repeated 3 times.
As shown in Figure 7, grafting PPO activity is in wavy variation tendency, and control seedling PPO activity is in first to increase to tend to be steady afterwards
Fixed trend.There are 3 peaks in 4d, 12d and 30d in grafting PPO activity, and value is respectively；35.35U/g FW·min、
43.32U/g FWmin, 42.49U/g FWmin are 122%, 172% and the 155% of its same time control respectively.Control
There is 1 peak in 4d in PPO activity, and PPO is 28.98U/g FWmin, is the 81.9% of its same time grafting respectively.
Work as cell damage, PPO activity will rise rapidly, and generates separation layer and protect the plants from injury, and separation layer
Effect is to prevent moisture evaporation, and wound is protected not invaded by harmful substance, and the peak that 4d occurs is exactly to protect the plants from
Injury, it is that separation layer forms the phase that can deduce 4d.Meanwhile PPO participates in the synthesis of lignin in callus reaction, helps to tie up pipe group
The connection knitted.In the present embodiment, there is peak in 12d and 30d in grafting PPO activity, and inventor speculates that 12d, 30d are graftings
The important period of lignin synthesis in agglutination, and lignin synthesis period includes callus is differentiated to form phase and vascular bundle
It is differentiated to form the phase, to judge that 12d is that callus is differentiated to form the phase, 30d is that vascular bundle is differentiated to form the phase.
It is ground by the above grafting and the control soluble sugar of seedling, relative conductivity, SOD, POD, CAT, PAL, PPO
Study carefully, inventor has found that 4d, 12d, 24d, 30d are the critical periods of grafting healing, these key points may be to be in graft union
Separation layer in agglutination forms the phase, callus is differentiated to form the phase, callus connection phase, forming layer are differentiated to form the phase and
Vascular bundle is differentiated to form the phase.
Wherein table 8 is the healing period table of various physical signs, and as shown in Table 8,4d had not only appeared in separation layer and formed the phase, but also
There is callus and be differentiated to form the phase, thus illustrates that 4d is that separation layer latter stage, callus are differentiated to form initial phase, i.e. 0~4d
The phase is formed for separation layer.12d had not only appeared in callus and has been differentiated to form the phase but also appears in the callus connection phase, can deduce
12d is that callus is differentiated to form phase in the end of term, callus connection initial phase.That is 4~12d is that callus is differentiated to form the phase.
18d is that callus connects the phase, therefore deduces the latter stage that 24d is the callus connection phase with 24d, and therefore, 12~24d is callus
Organize the connection phase.30d is that forming layer is differentiated to form the phase, and 30d, 36d are that vascular bundle is differentiated to form the phase, and 30~42d is also dimension
Tube bank is differentiated to form the phase, therefore it is that forming layer is differentiated to form the latter stage of phase, vascular bundle is differentiated to form initial stage of phase that can deduce 30d,
Thus show that 24~30d is that forming layer is differentiated to form the phase, 30~42d is that vascular bundle is differentiated to form the phase.
In conclusion inventor deduces Magnolia wufengensis graft union process is：0~4d is that separation layer forms the phase；4~
12d is that callus is differentiated to form the phase；12~24d is that callus connects the phase；24~30d is that forming layer is differentiated to form the phase；30
~42d is that vascular bundle is differentiated to form the phase.
Studies have shown that the graft union period of Magnolia wufengensis be 40 days or so, therefore in 40 days following measures implementation,
The graft union time can be shortened, graft survival rate is provided.Such as：It should be required to be made in strict accordance with grafting operation technology when grafting
Industry, The faster the better for operating process, should shorten the activity duration as possible, so that the polyphenol substance of production is reduced, can shorten isolation in this way
The layer time, graft union is enable to be differentiated to form the phase into callus faster.The water and fertilizer management that should reinforce stock before grafting, makes
It accumulates more nutrients, and scion also must be the full branch of robust growth, sprout, can be that provide the callus connection phase
More energy.Meanwhile giving graft union certain aeration condition, meet needed for the cambial cell respiration of anvil fringe engaging portion
The oxygen wanted promotes forming layer to break up, and so that agglutination is entered vascular bundle faster and breaks up period, to which whole shortening grafting is cured
Close the time.
The healing period table of 8 various physical signs of table
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiments or example.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changes, replacing and modification.
Claims (10)
1. a kind of Magnolia wufengensis engrafting method, which is characterized in that including：
Scion is grafted onto on stock, the stock includes being selected from one of Magnolia wufengensis and Y biondii(Pamp)D.L.Fu,
Wherein, the scion is red No. 1 tender.
2. according to the method described in claim 1, it is characterized in that, the stock is Y biondii(Pamp)D.L.Fu.
3. according to the method described in claim 1, it is characterized in that, the stock seedling age is life in 2~4 years.
4. according to the method described in claim 3, it is characterized in that, the stock seedling age is life in 4 years.
5. according to the method described in claim 1, it is characterized in that, the time of the grafting is 1 day~September of August 1 day.
6. according to the method described in claim 5, it is characterized in that, the time of the grafting is August 10~20, preferably
Ground, the grafting time are August 15.
7. according to the method described in claim 6, it is characterized in that, the temperature of the grafting is 19~34 DEG C.
8. the method according to the description of claim 7 is characterized in that the temperature of the grafting is 21~25 DEG C.
9. according to the method described in claim 1, it is characterized in that, the grafting is by simple bud side grafting, embedding bud, T font buds
It connects at least one of to connect with I-shaped bud and realize,
Preferably, the grafting is to connect realization by simple bud side grafting or T font buds.
10. a kind of Magnolia wufengensis engrafting method, which is characterized in that including：Using 2~4 years raw Magnolia wufengensis or Y biondii(Pamp)D.L.Fu as anvil
Wood, with tender red No. 1 for scion, described tender red No. 1 is ripe elite stand branch raw for the year, is connect using simple bud side grafting or T font buds
Mode is grafted, and the time of the grafting is 1 day~September of August 1, and the temperature of the grafting is 19~34 DEG C.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126418A (en) * | 2014-07-08 | 2014-11-05 | 安徽美心生态园林有限公司 | Southern magnolia grafting method |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105706823A (en) * | 2016-02-13 | 2016-06-29 | 余启佳 | Magnolia grafting method |
| CN106718114A (en) * | 2017-01-22 | 2017-05-31 | 湖北众森生态林业股份有限公司 | The immature seedling simple bud cut-grafting engrafting method of Magnolia wufengensis |
-
2017
- 2017-10-10 CN CN201710936259.0A patent/CN108293461A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104126418A (en) * | 2014-07-08 | 2014-11-05 | 安徽美心生态园林有限公司 | Southern magnolia grafting method |
| CN105009946A (en) * | 2015-07-08 | 2015-11-04 | 北京林业大学 | Grafting method of Magnolia wufengensis |
| CN105706823A (en) * | 2016-02-13 | 2016-06-29 | 余启佳 | Magnolia grafting method |
| CN106718114A (en) * | 2017-01-22 | 2017-05-31 | 湖北众森生态林业股份有限公司 | The immature seedling simple bud cut-grafting engrafting method of Magnolia wufengensis |
Non-Patent Citations (2)
| Title |
|---|
| 国家种苗网: "娇红1号的嫁接方法及接穗选择浅析", 《HTTP://WWW.SOHU.COM/A/191288164_273223》 * |
| 高新一等: "《果树林木嫁接技术手段》", 31 December 2014 * |
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