CN108279312A - The analytical equipment and Virus monitory method of a kind of proteomics based on nano-pore and application - Google Patents
The analytical equipment and Virus monitory method of a kind of proteomics based on nano-pore and application Download PDFInfo
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- CN108279312A CN108279312A CN201810191698.8A CN201810191698A CN108279312A CN 108279312 A CN108279312 A CN 108279312A CN 201810191698 A CN201810191698 A CN 201810191698A CN 108279312 A CN108279312 A CN 108279312A
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Abstract
The invention discloses a kind of analytical equipment of basic nano-pore proteomics and Virus monitory method and applications, device includes the fluid slot for filling solion, fluid slot is divided into two-part chip, the electrode of access fluid slot solution or chip, with the concatenated current amplifier of electrode and power supply, chip includes lower substrate layer and upper layer nano thin-film, it is provided with base bore 10 on basal layer, nano-pore is provided on nano thin-film.Application potential based on nano-pore in terms of sequencing, nano-pore proteomics will play increasingly important role in protein research, utilize the advantage of nano-pore, sensitivity and resolution capability of the present invention by design nano-pore, use nano-pore technology to carry out the direct detection of serum albumin mix；Technical scheme of the present invention has the characteristics that quick, highly sensitive, low cost, required sample size are few so that the albumen distribution in quick diagnosis microlitre rank serum is possibly realized.
Description
Technical field
The present invention relates to a kind of analytical equipment of basic nano-pore proteomics and Virus monitory method and applications, belong to
Bioanalysis and detection field.
Technical background
Either serum contains 5 kinds of high-abundance proteins and thousands of kinds of low medium-abundance low molecular weight proteins or peptide fragment to human plasma, and 5
Kind high-abundance proteins are respectively albumin, immunoglobulin, transferrins, haptoglobin and lipoprotein [1].Haemocyanin electricity
Swimming is the current common medical monitoring means for high abundance haemocyanin.Low molecular weight protein is secreted from tissue or cell
, or from larger haemocyanin degradation.It is special that low molecular weight protein is considered represent largely undeveloped diseases
Property diagnostic message, therefore it is considered biomarker.For brief, biomarker can be defined as instruction, and certain is specific
The molecule of disease.Outside isolating protein, biomarker can also be DNA or RNA molecule.Biomarker molecule is examined in cancer
It surveys, prejudges and prediction aspect is playing an increasingly important role.
Two-dimensional gel electrophoresis is the main method [2] of current separation analysis protein mixture, is contributed to we have appreciated that thin
The complexity and diversity of protein in born of the same parents and body fluid.Albumin is relatively dense in two-dimensional gel electrophoresis comparative analysis serum, blood plasma
The difference [2,3] of degree, has great importance [4] to the clinical diagnosis of part disease such as myeloma.However, although two dimension
Gel electrophoresis technology was achieving preferable development in recent years, but still had limitation, was mainly shown as：Required sample number
It measures more；It can not reuse；The dynamic range of protein detection is limited；For the extreme protein of molecular mass and isoelectric point
Not applicable [5].To solve above-mentioned limitation, a variety of separation methods are applied to the detection of proteomics with mass spectrographic joint technology
In [6,7].Due to the development of substance assistant laser desorpted/ionization technique, mass spectrum becomes the powerful of protein science, can be with
The information such as protein molecule size, primary structure parsing, posttranslational modification, protein interaction are provided, however are asked there are still several
Topic：First, high-abundance proteins flood low-abundance protein signal；Second is that protease is degraded；Third, protein is in ionization process
Dissociation [8].
In recent years, in molecular diagnostics and proteomics field, nano-sensor is as overdelicate sensing
Device and protein-enriched device show many unique and advantageous physical characteristics.Such as it is received using quantum dot [9] and plasma
Rice grain [10] is used as improved label and optical markings object, real-time biological sensor [11] of the nano wire as non-marked.But
It is that these methods are required for a receptor, such as antibody.Compared with based on two-dimensional gel electrophoresis or mass spectrographic proteomics, these
The unpredictable complete proteomic information of method.Therefore, exploitation is a kind of practicable, does not need receptor and can predict
The means of holoprotein group information are very important.
Bibliography
1.Adkins,J.N.,et al.,Towarda human bloodserumproteome-Analysis by
multidimensional separation coupledwith mass spectrometry.Molecular&Cellular
Proteomics,2002.1(12): p.947-955.
2.Anderson,L.and N.G.Anderson,High-Resolution 2-
DimensionalElectrophoresis of Human-Plasma Proteins.Proceedings ofthe
National Academy ofSciences ofthe United States ofAmerica,1977.74(12):p.5421-
5425.
3.Chromy,B.A.,et al.,Proteomic analysis ofhuman serum by two-
dimensional differential gel electrophoresis after depletion ofhigh-
abundantproteins.Journal ofProteome Research, 2004.3(6):p.1120-1127.
4.Bradwell,A.R.,et al.,Serum testfor assessment ofpatients with Bence
Jones myeloma. Lancet,2003.361(9356):p.489-491.
5.Issaq,H.J.and T.D.Veenstra,Two-dimensionalpolyacrylamide gel
electrophoresis (2D-PAGE):advances andperspectives.Biotechniques,2008.44(5):
p.697-+.
6.Aebersold,R.and M.Mann,Mass spectrometry-basedproteomics.Nature,
2003.422(6928): p.198-207.
7.Bensimon,A.,A.J.R.Heck,and R.Aebersold,Mass Spectrometry-
BasedProteomics and NetworkBiology.Annual Review ofBiochemistry,Vol 81,
2012.81:p.379-405.
8.Diamandis,E.P.,Mass Spectrometry as a diagnostic anda cancer
biomarker discovery tool -Opportunities andpotential limitations.Molecular&
Cellular Proteomics,2004.3(4):p. 367-378.
9.Rosi,N.L.and C.A.Mirkin,Nanostructures in biodiagnostics.Chemical
Reviews,2005. 105(4):p.1547-1562.
10.Wang,H.,et al.,Plasmonic nanostructures:Artificial
molecules.Accounts ofChemical Research,2007.40(1):p.53-62.
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markers with nanowire sensor arrays.Nature Biotechnology,2005.23(10):p.1294-
1301.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of proteomics methodologies based on nano-pore, realize
Rapid clinical Virus monitory and biomarker identification, improve the sensitivity of nano-pore detection, break through non-marked sensing
Limitation goes out new direction for bioassay technique Developing.
The present invention is achieved through the following technical solutions：
The invention discloses a kind of analytical equipment of the proteomics based on nano-pore, the device include fill from
Fluid slot is divided into two-part chip, the electrode of access fluid slot solution or chip, connected with electrode by the fluid slot of sub- solution
Current amplifier and power supply, chip includes lower substrate layer and upper layer nano thin-film, and base bore 10, nanometer are provided on basal layer
Nano-pore is provided on film.
As a further improvement, nano-pore aperture of the present invention is between 0-50nm, it is close with protein size,
Thickness is between 0.3-50nm.
As a further improvement, nano-pore of the present invention is solid nano hole or biological nano hole.
As a further improvement, solid nano Porous materials of the present invention include silicon nitride, silica, curing
Molybdenum, graphene, nano-pore have it is multiple, it is integrated by the parallelization arrays of multiple nanopore-channels.
As a further improvement, nano-pore of the present invention other than be covered with PDMS coats and/or it is described under
The material of layer basal layer is insulating materials.
As a further improvement, analytical equipment of the present invention further includes adding electric current on the both sides of nano thin-film,
It realizes and is read by increasing transverse circuit electric current molecule punch signal；Either analytical equipment further includes increasing above nano-pore
Optical detector, realize be used in combination optical detection means nano-pore molecule perforation behavior signal read.
As a further improvement, the preparation facilities of nano-pore of the present invention includes the fluid for filling solion
Slot, fluid slot is divided into two-part chip, chip includes upper layer nano thin-film and lower substrate, access fluid slot solution electricity
Pole, with the concatenated current amplifier of electrode and power supply, the power supply and current amplifier that are connected on nano thin-film, be provided on basal layer
Base bore 10.
As a further improvement, the preparation method of nano-pore of the present invention is:
1) oxidizing potential directly, is applied in nano-porous thin film by the power supply and current amplifier that are connected on nano thin-film,
0 oxidizing potential for being less than 100V is applied more than to nano-porous thin film, single solid-state is etched using controllable electrochemical reaction and receives
Metre hole；
2), using the electrode, independent with the concatenated current amplifier of electrode and this channel of power supply of access fluid slot solution
The feedback control that transmembrane conductance carries out oxidizing potential is measured, i.e., when set nano-pore transmembrane conductance reaches corresponding specific nanometer
When the aperture of hole, stops applying oxidizing potential, complete nano-pore preparation process.
The invention also discloses a kind of analytical equipment rapid clinicals of proteomics using nano-pore to detect serum
Method：
1), blood serum sample is pre-processed in buffer solution；
2) blood serum sample, is added in fluid slot solution, each albumen in blood serum sample passes through nanometer under electric field action
Hole；
3), pass through current amplifier record current perforation collection of illustrative plates；
4), by analyzing electric current perforation collection of illustrative plates and/or being hindered by the electric current that statistical analysis obtains by electric current perforation collection of illustrative plates
Conductance drop and/or aperture time and/or capture rate are filled in analyze detection serum.
As a further improvement, the present invention by some current blockade peak-to-average, estimates in corresponding human serum
The exclusion volume of certain corresponding albumen speculates protein molecular weight by exclusion volume, by two-dimensional parameter space conductance obstruction and
Aperture time effectively distinguishes the protein molecule type in human serum, and the concentration of the type protein is extrapolated from capture rate
Information.
The invention also discloses the applications that a kind of analytical equipment using proteomics finds biomarker, using this
Nano-pore analytical equipment analyzes the blood serum sample of a large amount of Healthy Human Serum sample and certain Disease, establishes database, leads to
The nano-pore for crossing comparison Healthy Human Serum albumen comes across the electric current collection of illustrative plates of electric current collection of illustrative plates and the blood serum sample of certain Disease
Difference is found, characteristic protein matter or molecule are found, finds biomarker.
The invention also discloses a kind of application of the analytical equipment detection and identification microorganism of proteomics of nano-pore,
Different microorganisms, different microbial lytic liquid, since protein molecular composition is variant are analyzed using the nano-pore analytical equipment
And formed with specificity nano-pore pass through electric current collection of illustrative plates, by software to the comparison of database can carry out microbial species or
The differentiation and identification of bacterial strain.
The present invention has the following technical effect that：
Application potential based on nano-pore in terms of sequencing, nano-pore proteomics will play in protein research
Increasingly important role, using the advantage of nano-pore, sensitivity and resolution capability of the present invention by design nano-pore use
Nano-pore technology carries out the direct detection of serum albumin mix；Technical scheme of the present invention have quickly, it is highly sensitive, low at
Originally the features such as, required sample size is few so that the albumen distribution in quick diagnosis microlitre rank serum is possibly realized.The present invention is based on
Nano-pore proteomics methodology detects the protein content in human serum, and estimates its molecular weight according to its collection of illustrative plates of perforating, by carrying
Take capture rate analysis protein relative concentration.In addition, can effectively reduce silicon nitride nano hole using PDMS coating pretreatments
Noise improves nano-pore signal-to-noise ratio to 10, has high resolution, human albumin when detecting human albumin's mixture
The conductance obstruction peak generated when perforation is consistent with its molecular size, blocks average value by electric current or conductance, it is estimated that
The exclusion volume of high-abundance proteins in human serum, is blocked and the aperture time by two-dimensional parameter space conductance, can effectively be distinguished
Several high-abundance proteins matter in human serum, and the detection of full low-abundance protein is also highly desirable to such resolution ratio in human serum,
The concentration information of protein can also be extrapolated from capture rate, the present invention can be applied to hospital laboratory to human serum
Clinical rapid diagnosis analysis, while providing the proteomics based on nano-pore for the discovery of biomarker in human serum and putting down
Platform.It is believed that nano-pore proteomics will become a strong tool in protein research field；It can also make
Other mixed solutions or body fluid are analyzed with the nanopore device：Such as interstitial fluid internal protein and other molecules, lymph
Deng using similar principles, using the other clinical examination index of nanopore device analysis.
Description of the drawings
Fig. 1 is the structural schematic diagram of analytical equipment of the present invention；
Fig. 2 is the structural schematic diagram that the present invention prepares nanopore device；
Fig. 3 is the structural schematic diagram that PDMS layer is coated on nano thin-film；
Fig. 4 is nano-pore haemocyanin electric current perforation collection of illustrative plates in the embodiment of the present invention；
Fig. 5 is results of statistical analysis figure of the embodiment of the present invention；
In figure, 1 is fluid slot, and 2 be electrode, and 3 be current amplifier, and 4 be power supply, and 5 be nano thin-film, and 6 be substrate, and 7 are
Protein, 8 be PDMS coats, and 9 be nano-pore, and 10 be base bore.
Specific implementation mode
The analytical equipment for 7 groups of protein based on nano-pore 9 that the invention discloses a kind of, Fig. 1 are present invention analysis dresses
The structural schematic diagram set；Device includes the fluid slot 1 for filling solion, divides fluid slot 1 for two-part chip, incoming stream
The electrode 2 of 1 solution of body slot or chip, with 2 concatenated current amplifier 3 of electrode and power supply 4, chip include 6 layers of lower substrate and
Upper layer nano thin-film 5 is provided with nano-pore 9 on nano thin-film 5,6 hole of substrate is provided in 6 layers of substrate, 9 aperture of nano-pore is in 0-
Close with 7 size of protein between 50nm, for thickness between 0.3-50nm, nano-pore 9 is solid nano hole 9 or biological nano
Hole 9,9 material of solid nano hole include silicon nitride, silica, molybdenum disulfide, graphene etc., and the nano-pore 9 can have more
It is a, it is integrated by the parallelization array in 9 channel of multiple nano-pores.9 geometry of solid nano hole by 9 material of nano-pore surface
Tension and the dynamic (dynamical) interaction of trepanning determine that it can be adjusted by focusing electron beam, and Fig. 3 is in nano thin-film 5
The structural schematic diagram of upper coating PDMS layer is provided with PDMS coats 8 and/or 6 layers of the lower substrate other than nano-pore 9
Material is insulating materials, insulating materials such as glass, quartz；Untreated solid nano hole 9 has strong noise, and two kinds main
Noise source be respectively the relevant high frequency noise of silicon base chip capacitance and with 1/f features low-frequency current fluctuation.Low noise
Than limiting resolution capability when processing mixed compound.The present invention uses the skill of polydimethylsiloxane coating film periphery
Art is to reduce dielectric noise.Using homemade experimental facilities, coating or photoetching technique determine that PDMS coats film location manually, very
Big degree improves 9 performance of nano-pore, and very high to-noise ratio is shown in electroporation experiments.The raising of signal-to-noise ratio makes human serum
The direct measurement of albumen is possibly realized.After PDMS is pre-processed, the nano-pore 9 for choosing low noise carries out electroporation experiments.
Analytical equipment can also be adds electric current on the both sides of nano thin-film 5, realizes by increasing transverse circuit electric current point
Sub- punch signal is read；Receiving for optical detection means is used in combination in the increased optical detector either above nano-pore 9, realization
9 molecule of metre hole perforation behavior signal is read.
Fig. 2 is the structural schematic diagram that the present invention prepares 9 device of nano-pore；The preparation facilities of nano-pore 9 includes filling ion
The fluid slot 1 of solution, to divide fluid slot 1 for two-part chip, chip include upper layer nano thin-film 5 and lower substrate 6, access
1 solution electrode 2 of fluid slot, with 2 concatenated current amplifier 3 of electrode and power supply 4, the power supply 4 and electric current that are connected on nano thin-film 5
The preparation method of amplifier 3, nano-pore 9 is:
1), thin in nano-pore 9 by power supply 4 and the current amplifier 3 directly application oxidizing potential being connected on nano thin-film 5
Film is applied more than 0 oxidizing potential for being less than 100V to 9 film of nano-pore, single consolidate is etched using controllable electrochemical reaction
State nano-pore 9；
2), using the electrode 2 of access 1 solution of fluid slot and 2 concatenated current amplifier 3 of electrode and this channel of power supply 4
Independent measurement transmembrane conductance carries out the feedback control of oxidizing potential, i.e., when set 9 transmembrane conductance of nano-pore reaches corresponding special
When determining 9 aperture of nano-pore, stops applying oxidizing potential, complete 9 preparation process of nano-pore.
This method can effectively prepare the nano-pore 9 in arbitrary aperture within 50nm, and this method is different from Feng etc.
The hanging electricity developed before (Nanopore formatting method and uses thereof, WO2016/142925A1)
2 electrochemical nano hole of pole, 9 preparation method, more quickly, inexpensively, mass production.
Since protein 7 needs to be unfolded that narrow biological hole (α-hemolysin pore could be passed through；MspA
Pore), therefore in 7 detection process of protein there is limitation physically.7 size of most of protein from 1 to 20nm not
Deng, therefore, it is necessary to by 9 aperture design of nano-pore to close with 7 size of protein.Compared with biological nano hole 9, solid nano hole
9 properties are more stable, are suitable for more liquid mediums and wider array of pH ranges, and with stronger operable in geometry dimension
Property, the protein 7 for differentiating different molecular quality and different isoelectric points can be blocked according to ionic current.Protein 7 passes through nanometer
The ionic current block signal in hole 9 can provide the exclusion volume information of corresponding protein 7.It further, can be by receiving
A molecular receptor (such as antibody) is anchored in metre hole 9 to increase chemo-selective, specific detection protein 7 measures its combination
Dynamics.
The invention also discloses a kind of analytical equipment rapid clinicals of 7 groups of protein using nano-pore 9 to detect serum
Method：
1), blood serum sample is pre-processed in the buffer solution that pH is 10-13；
2) blood serum sample, is added in 1 solution of fluid slot, each protein molecular in blood serum sample under electric field action one by one
Pass through nano-pore 9；
3) 9 haemocyanin of nano-pore perforation electric current collection of illustrative plates, is recorded by current amplifier 3；Different electric current drops or conductance
The difference of the perforation procedure of drop 7 molecule of corresponding single protein, 7 bulk of molecule of protein and charge causes single protein
The difference of 7 molecules perforation behavior：It is embodied in electric current range of decrease degree and aperture time length difference in this master drawing.
4), by analyzing electric current perforation collection of illustrative plates and/or being hindered by the electric current that statistical analysis obtains by electric current perforation collection of illustrative plates
Conductance drop and/or aperture time and/or capture rate are filled in analyze detection serum.The statistical result peak of difference drops in electric current conductance
Position corresponds to certain type of serum protein molecule respectively, can be compareed normally to a large amount of statistical analysis of serum perforation collection of illustrative plates
The difference of people and certain Disease find feature biomarker.
(electric current/conductance hinders for the characteristic signal peak position for statistically determining certain protein 7 using Gauss curve fitting
Plug), the histogram of conductance block signal caused by quantitative comparison high abundance serum albumin mix.Error line is Gaussian Profile
Standard deviation.It is dropped by the conductance of ionic current, can be evaluated whether the exclusion volume of perforation compound.An exclusion was proposed before this
The concept of volume, conductance drop are directly proportional to by the molecular exclusion volume of nano-pore 9.Albumen electricity can be calculated using following equation
Lead drop.
γ is form factor, and σ is electrical conductivity of solution, VexcludedFor the perforation exclusion volume of protein 7, HeqFor equivalent nanometer
9 thickness of hole, f are the ratio of correction factor, that is, relative porosity factor and molecular structure.
HeqIt is calculated using following formula
Heq=hp+0.8×dp
Wherein hpFor film thickness, dpFor pore size.
According to the average value that the conductance of different haemocyanins drop measures, the exclusion volume of different haemocyanins can be evaluated whether.
The result is consistent with expection, i.e. 7 exclusion volume of protein is bigger, and bigger drops in conductance.
By some current blockade peak (electric current drop) average value, the row of certain corresponding albumen in corresponding human serum is estimated
Resistance body accumulates, and 7 molecular weight of protein (molecular size) is speculated by exclusion volume, when blocking and perforate by two-dimensional parameter space conductance
Between, 7 molecular species of protein in human serum is effectively distinguished, the concentration information of the type protein 7 is extrapolated from capture rate.
The invention also discloses a kind of analytical equipments of 7 groups of protein using nano-pore 9 to find biomarker
Using analyzing the blood serum sample of a large amount of Healthy Human Serum sample and certain Disease using 9 analytical equipment of nano-pore, build
Vertical database, the nano-pore 9 by comparing Healthy Human Serum albumen pass through the blood serum sample of electric current collection of illustrative plates and certain Disease
Electric current collection of illustrative plates find difference, find characteristic protein matter 7 or molecule, find biomarker.
The invention also discloses a kind of analytical equipment detection and identification microorganisms of 7 groups of protein using nano-pore 9
Application, using 9 analytical equipment of nano-pore analyze different microorganisms, different microbial lytic liquid, due to protein molecular group
The nano-pore 9 with specificity is formed at variant and passes through electric current collection of illustrative plates, and the comparison of database can be carried out by software
The differentiation and identification of microbial species or bacterial strain.
Technical scheme of the present invention is further described below by specific embodiment：
It is completed in Superclean Lab, it is by dry etching and 100nm to select 380 micron thickness silicon chips, overlay coating
It is thin to be prepared into 50 square micron size 20nm thick silicon nitrides using potassium hydroxide wet etching for the silicon nitride of silica and 20nm
Film window prepares the nano-pore 9 of 0.3-50nm using transmission electron microscope or electrochemical method on film.It is assembled to fluid
After slot 1, entire fluid slot 1 uses 1：1 water and ethanol solution rinse are simultaneously impregnated at least 30 minutes, addition buffer solution (1M KCl,
10mM Tris-HCl, 1mM EDTA, pH 7.0) Current Voltage is detected afterwards, two silver chloride electrodes 2 are installed on two bufferings
In liquid pool, and Axopatch 200B patch clamp amplifiers are connected, for detecting ionic current, the function of time, which is applying
It needs operation to carry out blank assay at least one hour after voltage, blood serum sample is then wherein added again, is remembered by current amplifier 3
Electric current perforation collection of illustrative plates is recorded, Fig. 4 is 9 haemocyanin electric current of nano-pore perforation collection of illustrative plates schematic diagram in the embodiment of the present invention；In perforation electricity
Press 600mV, under conditions of 9 10nm of silicon nitride nano hole, protein 7, which is perforated, causes the interim obstruction of ionic current, show as from
The reduction of electron current, i.e., each obstruction peak in current time data, because the aperture of single nano-pore 9 only allows an albumen
7 molecule of matter passes through, so each obstruction peak corresponds to a perforation behavior of unimolecule protein 7, signal-to-noise ratio is higher than 10.Fig. 5 is this
Inventive embodiments results of statistical analysis figure；It is clearly seen corresponding to five kinds of high abundance serum eggs in analysis result drops in electric current
White peak, respectively albumin, 1-globulin, 2-globulin ,-globulin and-globulin.
Human albumin's perforation average current obstruction (current blockage) and its molecular mass (MW) correlated results；
Display current blockade increases with the increase of 7 molecular mass of protein, by the statistical analysis to 7 aperture time of protein,
It was found that its distribution follows simple one-dimensional biasing diffusion model, explain 7 aperture time of protein is presented in most cases
The phenomenon that Gaussian Profile, as a result, except through current blockade, we can also be mixed by aperture time distributional analysis protein 7
Close object.However, the aperture time is influenced by voltage, a relatively high voltage can show as better needle-shaped distribution, so
We are under relatively high voltage (600mV), the obstruction peak that generates when observing protein 7 is perforated.It is perforated using haemocyanin
Time (dwell time) and current blockade (current blockage) two-dimensional map (be respectively albumin, 1-
Globulin, 2-globulin ,-globulin and-globulin) 7 type of different proteins can be preferably distinguished, it perforates
The increase of time can further identify 7 mixture of protein more complicated in human serum, such as can be used as the low of biomarker
Abundance protein or polypeptide.Identify that biomarker has the early diagnosis of cancer and other fatal diseases in human serum
Further important meaning.Currently, utilizing Matrix-assisted laser desorption ionization MALDI TOF and two-dimentional gel electricity
Swimming PAGE technologies have found the important biomarker in part, such as α 1-microglobulin.In addition, from the perforation of protein 7
Collection of illustrative plates, we can also be analyzed by peak value probability of happening in human serum by the total concentration of capture rate calculations human albumin
The relative amount of different kinds of proteins 7, this method are equally applicable to analyzing and identifying for other serum (such as fetal calf serums).
Obviously, above-mentioned not limit the embodiments, for those of ordinary skill in the art, stated upper
Various forms of variations can be also made on the basis of bright, protection of the embodiment after these are varied still in the invention
In range.
Claims (12)
1. a kind of analytical equipment of the proteomics based on nano-pore, which is characterized in that the device includes filling ion
The fluid slot (1) of solution, fluid slot (1) is divided into two-part chip, access fluid slot (1) solution or chip electrode (2),
With electrode (2) concatenated current amplifier (3) and power supply (4), the chip includes lower substrate (6) layer and upper layer nanometer thin
Film (5) is provided with base bore (10) on the substrate (6) layer, nano-pore (9) is provided on the nano thin-film (5).
2. the analytical equipment of the proteomics according to claim 1 based on nano-pore, which is characterized in that described receives
Metre hole (9) aperture is between 0-50nm, and close with protein (7) size, thickness is between 0.3-50nm.
3. the analytical equipment of the proteomics of nano-pore according to claim 1 or 2, which is characterized in that described receives
Metre hole (9) is solid nano hole (9) or biological nano hole (9).
4. the analytical equipment of the proteomics of nano-pore according to claim 3, which is characterized in that the solid-state is received
Metre hole (9) material includes silicon nitride, silica, molybdenum disulfide, graphene, and the nano-pore (9) has multiple, is received by multiple
The parallelization array in metre hole (9) channel is integrated.
5. the analytical equipment of the proteomics of nano-pore according to claim 3, which is characterized in that the nano-pore
(9) material that PDMS coats (8) and/or the lower substrate (6) layer are covered with other than is insulating materials.
6. the analytical equipment of the proteomics of the nano-pore according to claims 1 or 2 or 4 or 5, which is characterized in that institute
The analytical equipment stated further includes adding electric current on the both sides of nano thin-film (5), realizes and is worn by increasing transverse circuit electric current molecule
Hole signal is read；Either the analytical equipment further includes the increased optical detector above nano-pore (9), and realization is combined and made
It is read with nano-pore (9) molecule perforation behavior signal of optical detection means.
7. the analytical equipment of the proteomics of the nano-pore according to claims 1 or 2 or 4 or 5, which is characterized in that institute
The preparation facilities for the nano-pore (9) stated includes the fluid slot (1) for filling solion, fluid slot (1) is divided into two-part core
Piece, the chip include upper layer nano thin-film (5) and lower substrate (6), access fluid slot (1) solution electrode (2) and electrode
(2) concatenated current amplifier (3) and power supply (4), the power supply (4) that is connected on nano thin-film (5) and current amplifier (3), institute
It is provided with base bore (10) on substrate (6) layer stated.
8. the analytical equipment of the proteomics of nano-pore according to claim 7, which is characterized in that the nano-pore
(9) preparation method is:
1) oxidizing potential directly, is applied in nano-pore by the power supply (4) and current amplifier (3) that are connected on nano thin-film (5)
(9) film is applied more than 0 oxidizing potential for being less than 100V to nano-pore (9) film, list is etched using controllable electrochemical reaction
A solid nano hole (9)；
2), using the electrode (2) of access fluid slot (1) solution, with electrode (2) concatenated current amplifier (3) and power supply (4) this
One channel independent measurement transmembrane conductance carries out the feedback control of oxidizing potential, i.e., when set nano-pore (9) transmembrane conductance reaches
When to the specific nano-pore of correspondence (9) aperture, stops applying oxidizing potential, complete nano-pore (9) preparation process.
9. a kind of analytical equipment of proteomics using the nano-pore as described in claims 1 or 2 or 4 or 5 or 8 quickly faces
The method of bed detection serum：
1), blood serum sample is pre-processed in buffer solution；
2) blood serum sample, is added in fluid slot (1) solution, each albumen in blood serum sample passes through nano-pore under electric field action
(9)；
3), pass through current amplifier (3) record current perforation collection of illustrative plates；
4), the current blockade electricity by analyzing electric current perforation collection of illustrative plates and/or being obtained by statistical analysis by electric current perforation collection of illustrative plates
Drop and/or aperture time and/or capture rate are led to analyze detection serum.
10. the method for rapid clinical detection serum according to claim 9, which is characterized in that pass through some current blockade
Peak-to-average, estimates the exclusion volume of certain corresponding albumen in corresponding human serum, speculates protein (7) point by exclusion volume
Son amount, is blocked and the aperture time by two-dimensional parameter space conductance, effectively distinguishes protein (7) molecular species in human serum,
The concentration information of the type protein (7) is extrapolated from capture rate.
11. a kind of analytical equipment of proteomics using the nano-pore as described in claims 1 or 2 or 4 or 5 or 8 is found
The application of biomarker analyzes a large amount of Healthy Human Serum sample using nano-pore (9) analytical equipment and certain disease is suffered from
The blood serum sample of person, establishes database, and the nano-pore (9) by comparing Healthy Human Serum albumen passes through electric current collection of illustrative plates and certain disease
The electric current collection of illustrative plates of the blood serum sample of patient finds difference, finds characteristic protein matter (7) or molecule, finds biomarker.
12. a kind of analytical equipment of proteomics using the nano-pore as described in claims 1 or 2 or 4 or 5 or 8 detects
With identification microorganism application, using nano-pore (9) analytical equipment analyze different microorganisms, different microbial lytic liquid,
It forms the nano-pore (9) with specificity since protein molecular forms variant and passes through electric current collection of illustrative plates, by software to data
The comparison in library can carry out the differentiation and identification of microbial species or bacterial strain.
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| CN113588988A (en) * | 2021-06-21 | 2021-11-02 | 东南大学 | Protein stretching sequencing platform with two-dimensional plane heterostructure and preparation method thereof |
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| CN110607231A (en) * | 2019-10-19 | 2019-12-24 | 上海新微技术研发中心有限公司 | Gene transfer speed control device based on movement protein |
| WO2021209073A1 (en) * | 2020-04-13 | 2021-10-21 | 南京大学 | Nanopore channel monomolecular protein sequencer |
| GB2607542A (en) * | 2020-04-13 | 2022-12-07 | Nanjing University | Nanopore channel monomolecular protein sequencer |
| CN113588988A (en) * | 2021-06-21 | 2021-11-02 | 东南大学 | Protein stretching sequencing platform with two-dimensional plane heterostructure and preparation method thereof |
| CN113548641A (en) * | 2021-07-27 | 2021-10-26 | 中国科学院重庆绿色智能技术研究院 | Preparation method of confined dielectric breakdown solid-state nanopore device, product and application thereof |
| CN113548641B (en) * | 2021-07-27 | 2023-06-23 | 中国科学院重庆绿色智能技术研究院 | Preparation method of confined dielectric breakdown solid-state nano-pore device, product and application thereof |
| CN113686235A (en) * | 2021-08-16 | 2021-11-23 | 中国科学院力学研究所 | Method for estimating protein conformation morphology characteristics based on nanopore via hole current |
| CN115266876A (en) * | 2022-06-22 | 2022-11-01 | 浙江大学 | Graphene-molybdenum disulfide heterojunction nanopore biosensor structure and preparation method thereof |
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