CN108276584B - Method for detecting aromatic amine compound in human urine - Google Patents
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Abstract
The invention relates to a method for detecting aromatic amine compounds in human urine. The method comprises the steps of pretreating human urine, adding sulfonic acid group functionalized COFs materials for solid phase extraction, separating out solid phase substances, and eluting the solid phase substances by using an elution solvent to obtain eluent; measuring aromatic amine compound in the eluate; the sulfonic group functionalized COFs material comprises a covalent organic framework formed by carrying out Schiff base condensation reaction on 2,4, 6-trimethylacyl phloroglucinol and 2, 5-diaminobenzene sulfonic acid. The method takes the sulfonic functionalized COFs as the adsorbent to perform solid-phase extraction on the aromatic amine compounds in the human urine, can selectively and efficiently enrich the trace aromatic amine compounds in the complex matrix by utilizing the characteristics of the COFs, can greatly simplify the pretreatment process of the sample, and improves the detection efficiency and the detection accuracy of the aromatic amine compounds.
Description
Technical Field
The invention belongs to the field of detection of aromatic amine compounds, and particularly relates to a method for detecting aromatic amine compounds in human urine.
Background
Some aromatic amine compounds have carcinogenic and mutagenic effects and can cause poisoning by inhalation, ingestion, or absorption through the skin. The list of harmful substances and Potential Hazardous Substances (HPHCs) in the cigarette products and cigarette smoke of FDA contains 6 kinds of aromatic amine, 4-aminobiphenyl, 1-aminonaphthalene, 2-aminonaphthalene, o-anisidine, 2, 6-dimethylaniline and o-toluidine. These aromatic amine compounds are used as cigarette smoke exposure biomarkers.
The study of biomarkers is useful to elucidate the nature and extent of health risks when humans are exposed to such substances. Due to the fact that the content of the aromatic amine biomarker is low, interference is large during detection, and requirements for pretreatment of a sample are high. The urine can effectively reflect the recent multi-path exposure of environmental pollutants, and has great significance for the analysis and research of aromatic amine compounds in the urine. Grimmer et al (Detection of pathological aromatic amines in the urine of non-scanners. Sci Total Environ,2000,247(1):81-90) treated urine enzymatically and analyzed the aromatic amines in the urine using pentafluoropropionic anhydride derivatization and GC-MS. Riedel et al (Determination of tertiary carbonaceous amides in urine of smokers and nonsmokers. J Anal Toxicol,2006,30(3):187-195) detected sigma-toluidine, 2-aminonaphthalene and 4-aminobiphenyl in urine samples from smokers and non-smokers using the derivatization-GC-MS method. The GC-MS method requires derivatization reaction, and the pretreatment of a sample is very complicated and time-consuming.
Patent application with publication number CN102788852A discloses a method for detecting aromatic amine compounds in human urine by liquid chromatography-tandem mass spectrometry, which comprises hydrolyzing urine with concentrated HCl, purifying with PAH molecular imprinting column, and analyzing with LC-MS/MS. The method can simplify the sample pretreatment steps and improve the analysis efficiency, but the commercialized PAH molecularly imprinted column is a Polycyclic Aromatic Hydrocarbon (PAHs) molecularly imprinted column, is not an aromatic amine molecularly imprinted column, and has the problem of low selectivity.
In the existing detection method of aromatic amine compounds, the selective adsorption of an adsorbent to the aromatic amine compounds is poor, so that the detection efficiency and the detection accuracy of the aromatic amine compounds need to be improved.
Disclosure of Invention
The invention aims to provide a method for detecting aromatic amine compounds in human urine, so as to solve the problems of poor detection efficiency and poor detection accuracy of the existing detection method.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for detecting aromatic amine compounds in human urine comprises the following steps: after human urine is pretreated, adding sulfonic acid group functionalized COFs materials for solid phase extraction, separating out solid phase substances, and eluting the solid phase substances by using an elution solvent to obtain an eluent; measuring aromatic amine compound in the eluate;
the sulfonic group functionalized COFs material comprises a covalent organic framework formed by carrying out Schiff base condensation reaction on 2,4, 6-trimethylacyl phloroglucinol and 2, 5-diaminobenzene sulfonic acid.
The method for detecting the aromatic amine compound in the human urine takes the sulfonic group functionalized COFs material as the adsorbent to perform solid-phase extraction on the aromatic amine compound in the human urine, and can play the following roles: the pore size limitation effect of the COFs material can eliminate the adsorption of macromolecules such as protein in a urine matrix; the electrostatic adsorption effect of sulfonic groups on the COFs material and amino groups of aromatic amine compounds is strong, so that the aromatic amine compounds can be efficiently adsorbed, and the adsorption of electronegative compounds is prevented; the benzene ring structure on the COFs material and the benzene ring of the aromatic amine compound have strong pi-pi effect, and the selective and efficient enrichment of the trace aromatic amine compound in the complex matrix is realized under the combined action of the above factors. The COFs material is applied to the detection of the aromatic amine compound in the human urine, so that the pretreatment process of a sample can be greatly simplified, and the detection efficiency and the detection accuracy of the aromatic amine compound are improved.
The sulfonic group functionalized COFs material is prepared by the method comprising the following steps: carrying out Schiff base condensation reaction on 2,4, 6-trimethylacyl phloroglucinol and 2, 5-diaminobenzene sulfonic acid in a solvent to obtain the compound. Preferably, the weight ratio of the 2,4, 6-triacyl phloroglucinol to the 2, 5-diaminobenzene sulfonic acid is (10-30): (10-40).
The amino functionalized magnetic nanoparticles can be further added in the Schiff base condensation reaction, and the magnetic nanoparticles are modified on a covalent organic framework through the reaction of amino and aldehyde groups, so that the covalent organic framework has certain magnetism, and the magnetic sulfonic acid group functionalized COFs material is formed.
The magnetic sulfonic group functionalized COFs material comprises a covalent organic framework formed by carrying out Schiff base condensation reaction on 2,4, 6-triacyl trimesic phenol and 2, 5-diaminobenzene sulfonic acid, and magnetic nanoparticles modified on the covalent organic framework, wherein the magnetic nanoparticles are Fe with amino groups functionalized3O4/SiO2Core-shell nanoparticles.
Said amino-functionalized Fe3O4/SiO2Core-shell nanoparticles with Fe3O4As a core, with SiO2For the shell, preparation can be carried out according to the prior art. Preferably, the amino-functionalized Fe3O4/SiO2The core-shell nano-particles are prepared by a method comprising the following steps:
a) with nano Fe3O4The particles are used as the core, ethyl orthosilicate is used as a silicon source, and the stober method is adopted to prepare Fe3O4@SiO2Core-shell nanoparticles;
b) mixing Fe3O4@SiO2Dispersing the core-shell nano particles in toluene, and adding a toluene solution of 3-aminopropyl trimethoxy silane for coupling reaction to obtain the nano-particles.
In step a), each 1.0-4.0g of nano Fe3O4The particles were used in a volume of 1-5mL of tetraethyl orthosilicate. The stober method adopts a mixed solvent which is prepared from absolute ethyl alcohol, water and concentrated ammonia water according to the volume ratio of (50-200): (20-60): (1-5) and the concentration of the strong ammonia water is 25-28 wt%.
Preferably, the nano Fe3O4The particles are made by a process comprising the steps of: FeCl3·6H2Carrying out hydrothermal reaction on O and anhydrous sodium acetate in an ethylene glycol solvent to obtain the nano Fe3O4Particles. FeCl3·6H2O and do notThe mass ratio of the sodium acetate hydrate is (1.0-3.0): (2.0-5.0). The hydrothermal reaction is carried out at the temperature of 190 ℃ and 210 ℃ for 5-10 h.
In step b), 0.2-2.0g Fe per unit3O4@SiO2The core-shell nano particles are correspondingly added with 5-20mL of toluene solution of 3-aminopropyl trimethoxy silane, and the mass concentration of the toluene solution of 3-aminopropyl trimethoxy silane is 5-20%. The coupling reaction is reflux reaction at 100-120 ℃ for 20-30 h.
The weight ratio of the 2,4, 6-triacyl phloroglucinol, the 2, 5-diaminobenzene sulfonic acid and the magnetic nano particles is (10-30): (10-40): (10-50). The selective enrichment effect of the COFs material prepared by the reaction according to the proportion reaches the best.
The preparation method of the magnetic sulfonic acid group functionalized COFs material can adopt the following technical scheme:
a preparation method of magnetic sulfonic functionalized COFs materials comprises the following steps: performing Schiff base condensation reaction on 2,4, 6-trimethylacyl phloroglucinol, 2, 5-diaminobenzene sulfonic acid and magnetic nanoparticles in a solvent to obtain the magnetic nanoparticle.
The solvent is trimethylbenzene and dioxane in a volume ratio of 1: (3-6).
The Schiff base condensation reaction is carried out under the condition of taking acetic acid as a catalyst. Preferably, the mass of the added acetic acid is 5-50% of the total mass of the 2,4, 6-trimethylacylphloroglucinol, the 2, 5-diaminobenzene sulfonic acid and the magnetic nanoparticles.
The Schiff base condensation reaction is carried out for 60-80h at 90-120 ℃.
The magnetic sulfonic group functionalized COFs material prepared by adopting the optimized parameters has good product quality stability, has excellent selective and efficient enrichment effect on aromatic amine compounds in urine, and can be conveniently used in the analysis and detection process of the aromatic amine compounds in human urine.
The magnetic sulfonic functional COFs material has good application in magnetic solid phase extraction.
The magnetic sulfonic functional COFs material contains magnetic nanoparticles, after aromatic amine compounds are selectively enriched, phase separation can be achieved through an external magnetic field, the operation is simple and rapid, the detection process of the aromatic amine compounds is greatly simplified, a large amount of time is saved, and the cost is reduced.
Taking magnetic solid-phase extraction of aromatic amine compounds in human urine as an example, after concentrated hydrochloric acid treatment or enzymolysis treatment, adding a magnetic sulfonic group functionalized COFs material to perform selective enrichment of the aromatic amine compounds, separating the adsorbed magnetic sulfonic group functionalized COFs material by using a magnetic field effect, then eluting by using an elution solvent, and using the obtained eluent as a sample solution.
In the magnetic solid-phase extraction process, the magnetic sulfonic group functionalized COFs material can be conveniently separated from the liquid phase by using a magnet outside the container, and the processes of washing, elution and the like are greatly simplified.
The invention also provides application of the magnetic sulfonic functionalized COFs material in detection of aromatic amine compounds in human urine.
After human urine is pretreated, adding a magnetic sulfonic acid group functionalized COFs material for magnetic solid-phase extraction, separating the magnetic sulfonic acid group functionalized COFs material adsorbed with the object to be detected under the action of a magnetic field, and eluting by using an elution solvent to obtain an eluent; the eluate was concentrated and subjected to HPLC-MS/MS analysis.
The pretreatment is to add concentrated hydrochloric acid into human urine, treat the human urine at 60-80 ℃ for 0.5-2h, add NaOH solution to adjust the pH value to be neutral, and then add ammonium acetate-ammonia water buffer solution with the pH value of 7.0 to mix.
Preferably, 0.1-1mL of concentrated hydrochloric acid with the mass concentration of 36-38% is added into every 1-5mL of human urine. Adding 5-10mL of ammonium acetate-ammonia water buffer solution into 1-5mL of human urine; the mass of the functional COFs material added with the magnetic sulfonic group is 100-500 mg.
The time of magnetic solid phase extraction is 0.5-1 h. The elution solvent is formed by diluting strong ammonia water with methanol, the concentration of the strong ammonia water is 25-28 wt%, and the concentration of ammonia in the elution solvent is 2-7 wt%. Eluting with 5-20mL of eluting solvent, and concentrating the eluate to 0.1-1 mL.
The method is used for detecting the aromatic amine compound in the human urine, the cost of the magnetic sulfonic group functionalized COFs material is greatly reduced compared with that of a commercial solid-phase extraction column, the detection flow is greatly simplified, and the detection efficiency and the detection accuracy of the aromatic amine compound are improved.
Drawings
FIG. 1 is a schematic diagram of the synthesis of magnetic sulfonic acid group functionalized COFs materials;
FIG. 2 is a schematic diagram of the operation of the magnetic solid phase extraction process.
Detailed Description
The following examples are provided to further illustrate the practice of the invention. The magnetic nanoparticles in the following examples were prepared as follows:
a) 1.35g of FeCl3·6H2Dissolving O and 3.6g of anhydrous sodium acetate into 35mL of ethylene glycol, magnetically stirring the mixture until the mixture is dissolved in a solid phase, transferring the solution into a hydrothermal reaction kettle, carrying out hydrothermal reaction at 200 ℃ for 8 hours, carrying out solid-liquid separation, washing by using dilute hydrochloric acid with the concentration of 0.1mol/L, and drying to obtain the nano Fe3O4Particles;
b) nano Fe3O4Uniformly dispersing particles (4.0g) into a mixed solution consisting of absolute ethyl alcohol (160mL), deionized water (40mL) and concentrated ammonia water (28 wt%, 3mL), dropwise adding TEOS (2mL) under stirring to react to obtain Fe3O4@SiO2Nanoparticles;
c) 1.0g of Fe3O4@SiO2Nanoparticles dispersed in 100mL of anhydrous toluene in N2Under the protection, 10mL of anhydrous toluene solution of 10 wt% 3-aminopropyl trimethoxy silane is dripped, and the reflux reaction is carried out for 24h at 110 ℃, thus obtaining the amino functionalized Fe3O4/SiO2Core-shell nanoparticles.
Example 1
The magnetic sulfonic acid group functionalized COFs material of the embodiment is prepared by the following steps as shown in FIG. 1:
dissolving 2,4, 6-trimethyloylphloroglucinol (21.0mg) and 2, 5-diaminobenzene sulfonic acid (28.2mg) in a trimethylbenzene/dioxane mixed solvent (formed by mixing 0.2mL of trimethylbenzene and 0.8mL of dioxane), adding 0.1mL of an acetic acid solution with a concentration of 3mol/L, and mixing at room temperature to form a uniform solution; and then under the protection of nitrogen, adding 0.02g of magnetic nanoparticles, reacting for 72h at 100 ℃, separating out a black solid product after reaction, washing with anhydrous dimethylformamide, anhydrous tetrahydrofuran and anhydrous acetone in sequence, washing with anhydrous methanol for 2-3 times to complete activation, and drying in vacuum for 12h at 150 ℃ to obtain the magnetic sulfonic group functionalized COFs material.
The application of the magnetic sulfonic group functionalized COFs material in the embodiment to the detection of the aromatic amine compound in human urine comprises the following steps:
1) magnetic solid-phase extraction: taking a urine sample stored at-18 ℃ and unfreezing the urine sample at room temperature, putting 5mL of the unfrozen urine sample into a plastic centrifuge tube, adding 1mL of concentrated hydrochloric acid solution, treating the urine sample in a 80 ℃ constant-temperature water bath for 2 hours, then cooling the urine sample to the room temperature, adding 1.2mL of NaOH solution with the concentration of 10mol/L to neutralize the solution until the pH value is 7.0, then adding 10mL of ammonium acetate-ammonia water buffer solution with the concentration of 0.5mol/L, pH-7.0, and uniformly mixing the ammonium acetate-ammonia water buffer solution to obtain a sample solution;
transferring the sample solution into a 50mL beaker, adding 100 mu L D7-1-aminonaphthalene (with the concentration of 50ng/mL) and D9-4-aminobiphenyl (with the concentration of 10ng/mL) mixed internal standard solution and 100mg of magnetic sulfonic acid group functionalized COFs material, then magnetically stirring for 30min, placing a magnet on the outer wall of one side of the beaker to separate the adsorbed magnetic sulfonic acid group functionalized COFs material from the solution, and pouring the solution in the beaker; removing the magnet, adding 10mL of water into the beaker for washing, magnetically stirring for 10min, separating the phases by using the magnet again, and pouring out the washing water; adding 10mL of an elution solvent (prepared by diluting strong ammonia water with methanol, wherein the concentration of the strong ammonia water is 28 wt%, and the concentration of ammonia in the elution solvent is 5 wt%) into a beaker, magnetically stirring for 10min, separating the mixture by using a magnet, and pouring and collecting the eluent; concentrating the eluate to 0.1mL, and using the concentrated eluate as a solution to be tested for later use, wherein the process schematic diagram is shown in FIG. 2;
2) HPLC-MS/MS analysis: and (3) establishing a standard working curve of each target compound by using an internal standard method, analyzing the solution to be detected by using an HPLC-MS/MS instrument, substituting the detection data into the standard working curve, and calculating to obtain the concentrations of the 1-aminonaphthalene, the 2-aminonaphthalene, the 3-aminobiphenyl and the 4-aminobiphenyl in the sample.
In the step 2), the chromatographic conditions of HPLC-MS/MS analysis are as follows: spectral column was Waters C18(100mm × 2.1mm i.d.,2.7 μm), column temperature: 30 ℃; the mobile phase A is an aqueous solution containing 0.1 percent of formic acid, and the mobile phase B is an acetonitrile solution containing 0.1 percent of formic acid; gradient elution was used, conditions were as follows: 0-3min, 100% A0% B; 3.1-16.0min, 75% A25% B; 16.1-20min, 0% A and 100% B; 20.1-25.0min, 100% A; the sample injection volume is 10 mu L; the flow rate was 200. mu.L/min.
The mass spectrum conditions are as follows: an ion source: electrospray ionization source (ESI); the scanning mode is as follows: scanning positive ions; the detection mode is as follows: multiple reaction monitoring, MRM; electrospray voltage: 5500V; air curtain pressure: 30 psi; pressure of auxiliary gas 1: 70 psi; pressure of auxiliary gas 2: 70 psi; ion source temperature: 500 ℃; the quantitative ion pair, the qualitative ion pair, the residence time, the Collision Energy (CE) and the declustering voltage (DP) for each compound are shown in table 1. TABLE 1 quantitative ion-pair, qualitative ion-pair, residence time, collision energy and declustering voltage for each compound
Table 2 shows the linear equation, correlation coefficient, detection limit and quantitation limit of each aromatic amine compound with 3 times the signal-to-noise ratio (S/N) as the detection limit and 10 times the S/N as the quantitation limit.
TABLE 2 Linear equation, correlation coefficient, detection limit and quantitation limit for aromatic amine compounds
The same urine sample was subjected to 6 replicates per day and 5 days apart, and the Relative Standard Deviation (RSD) of the measurement results was shown in the results of the standard recovery test, together with the day precision and day time precision of the test method, and the results are shown in table 3.
TABLE 3 Intra-day precision, Interday precision and recovery of the method of example 1
As can be seen from the results in Table 3, the method has the advantages of good precision and high recovery rate of the added standard, and is suitable for detecting the aromatic amine metabolites in the human urine.
Urine samples of 5 smokers and 2 non-smokers were collected, and the metabolites of 1-aminonaphthalene, 2-aminonaphthalene, 3-aminobiphenyl and 4-aminobiphenyl in the urine samples were subjected to magnetic solid phase extraction and HPLC-MS/MS analysis by the method of example 1, and the results are shown in Table 4.
TABLE 4 urine sample analysis (pg/mL) for smokers and non-smokers
It can be seen that the method of example 1 can be used for conveniently detecting the content of metabolites of 1-aminonaphthalene, 2-aminonaphthalene, 3-aminobiphenyl and 4-aminobiphenyl in human urine, and has the advantages of simple detection process, good detection precision and stability; in table 4, the content of aromatic amine metabolites in the urine of smokers is significantly higher than that of non-smokers, and the purified treatment solution obtained by using the magnetic solid phase extraction technology can also lay a good foundation for the subsequent research of aromatic amine compounds.
Example 2
The magnetic sulfonic acid group functionalized COFs material of the embodiment is prepared by the following steps:
dissolving 2,4, 6-trimethyloylphloroglucinol (11.0mg) and 2, 5-diaminobenzene sulfonic acid (18.2mg) in a trimethylbenzene/dioxane mixed solvent (formed by mixing 0.2mL of trimethylbenzene and 0.6mL of dioxane), adding 0.1mL of an acetic acid solution with a concentration of 3mol/L, and mixing at room temperature to form a uniform solution; and then under the protection of nitrogen, adding 0.02g of magnetic nanoparticles, reacting for 80h at 90 ℃, separating out a black solid product after the reaction, washing with anhydrous dimethylformamide, anhydrous tetrahydrofuran and anhydrous acetone in sequence, washing with anhydrous methanol for 2-3 times to complete activation, and drying in vacuum for 12h at 150 ℃ to obtain the magnetic sulfonic group functionalized COFs material.
The method for detecting the aromatic amine compound in the human urine by applying the COFs of the embodiment can refer to the embodiment 1, and the specific detection effect is equivalent to that of the embodiment 1.
Example 3
The magnetic sulfonic acid group functionalized COFs material of the embodiment is prepared by the following steps:
dissolving 2,4, 6-trimethyloylphloroglucinol (28.0mg) and 2, 5-diaminobenzene sulfonic acid (40.0mg) in a trimethylbenzene/dioxane mixed solvent (formed by mixing 0.2mL of trimethylbenzene and 1.2mL of dioxane), adding 0.1mL of an acetic acid solution with a concentration of 3mol/L, and mixing at room temperature to form a uniform solution; and then under the protection of nitrogen, adding 0.04g of magnetic nanoparticles, reacting for 60 hours at 120 ℃, separating out a black solid product after the reaction, washing with anhydrous dimethylformamide, anhydrous tetrahydrofuran and anhydrous acetone in sequence, washing with anhydrous methanol for 2-3 times to complete activation, and drying in vacuum for 12 hours at 150 ℃ to obtain the magnetic sulfonic group functionalized COFs material.
The method for detecting the aromatic amine compound in the human urine by applying the COFs of the embodiment can refer to the embodiment 1, and the specific detection effect is equivalent to that of the embodiment 1.
Example 4
The sulfonic acid group functionalized COFs material of the embodiment is prepared by the following steps: dissolving 2,4, 6-trimethyloylphloroglucinol (21.0mg) and 2, 5-diaminobenzene sulfonic acid (28.2mg) in a trimethylbenzene/dioxane mixed solvent (formed by mixing 0.2mL of trimethylbenzene and 0.8mL of dioxane), adding 0.1mL of an acetic acid solution with a concentration of 3mol/L, and mixing at room temperature to form a uniform solution; and then reacting for 72h at 100 ℃ under the protection of nitrogen, separating out a solid product after the reaction, washing with anhydrous dimethylformamide, anhydrous tetrahydrofuran and anhydrous acetone in sequence, washing with anhydrous methanol for 2-3 times to complete activation, and vacuum drying for 12h at 150 ℃ to obtain the sulfonic group functionalized COFs material.
When the sulfonic acid group functionalized COFs material of example 4 is applied to detection of aromatic amine compounds in human urine, the specific steps can refer to example 2, and the difference is only that in step 1), the adsorbed sulfonic acid group functionalized COFs material is separated from a liquid phase by using a conventional solid-liquid separation means (such as filtration, centrifugation and the like), and then is eluted by using an elution solvent to obtain an eluent for subsequent analysis, and based on the structural characteristics of the sulfonic acid group functionalized COFs, the sulfonic acid group functionalized COFs material maintains good selective and efficient enrichment characteristics for aromatic amine compounds in human urine, so that the method can be applied to high-sensitivity and high-efficiency detection.
In other embodiments of the sulfonic acid group functionalized COFs material of the present invention, the amounts of 2,4, 6-triacyl trimesic phenol, 2, 5-diaminobenzene sulfonic acid, magnetic nanoparticles and the catalyst acetic acid, and the specific conditions of Schiff base condensation reaction can be adaptively adjusted within the range defined by the present invention, so that COFs materials with equivalent performance can be obtained.
Claims (9)
1. A method for detecting aromatic amine compounds in human urine is characterized by comprising the following steps: after human urine is pretreated, adding sulfonic acid group functionalized COFs materials for solid phase extraction, separating out solid phase substances, and eluting the solid phase substances by using an elution solvent to obtain an eluent; measuring aromatic amine compound in the eluate;
the sulfonic group functionalized COFs material comprises a covalent organic framework formed by carrying out Schiff base condensation reaction on 2,4, 6-triacyl phloroglucinol and 2, 5-diaminobenzene sulfonic acid, wherein the weight ratio of the 2,4, 6-triacyl phloroglucinol to the 2, 5-diaminobenzene sulfonic acid is (10-30): (10-40).
2. The method according to claim 1, wherein the sulfonic acid group functionalized COFs material is modified to the co-polymerMagnetic nanoparticles on a valence organic framework, the magnetic nanoparticles being amino functionalized Fe3O4/SiO2Core-shell nanoparticles.
3. The method according to claim 2, wherein the sulfonic acid group functionalized COFs material is prepared by a method comprising the following steps: performing Schiff base condensation reaction on 2,4, 6-trimethylacyl phloroglucinol, 2, 5-diaminobenzene sulfonic acid and magnetic nanoparticles in a solvent to obtain the magnetic nanoparticle.
4. The method according to claim 3, wherein the solvent is trimethylbenzene and dioxane in a volume ratio of 1: (3-6).
5. The method according to claim 3, wherein the weight ratio of the 2,4, 6-trimethyloylphloroglucinol, the 2, 5-diaminobenzenesulfonic acid and the magnetic nanoparticles is (10-30): (10-40): (10-50).
6. The method according to claim 1 or 3, wherein the Schiff base condensation reaction is carried out at 90-120 ℃ for 60-80 h.
7. The method for detecting the aromatic amine compound in the human urine according to claim 1, wherein the pretreatment comprises adding concentrated hydrochloric acid to the human urine, treating the human urine at 60 to 80 ℃ for 0.5 to 2 hours, adding NaOH solution to adjust the pH value to be neutral, and then adding ammonium acetate-ammonia buffer solution with pH of 7.0 to mix.
8. The method according to claim 1, wherein the eluting solvent is prepared by diluting strong ammonia with methanol, the concentration of strong ammonia is 25-28 wt%, and the concentration of ammonia in the eluting solvent is 2-7 wt%.
9. The method according to claim 1, wherein the measurement is performed by concentrating the eluate and performing HPLC-MS/MS analysis.
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