CN108276472A - A method of the high efficiency extraction Purslane plants selenoprotein from Purslane plants - Google Patents
A method of the high efficiency extraction Purslane plants selenoprotein from Purslane plants Download PDFInfo
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- CN108276472A CN108276472A CN201711471346.XA CN201711471346A CN108276472A CN 108276472 A CN108276472 A CN 108276472A CN 201711471346 A CN201711471346 A CN 201711471346A CN 108276472 A CN108276472 A CN 108276472A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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Abstract
The method of the invention discloses a kind of from Purslane plants high efficiency extraction Purslane plants selenoprotein, screens qualified selenium-rich Purslane plants, and clean up；Clean selenium-rich Purslane plants are dried using the method that drying or the sun are dried；By the selenium-rich Purslane plants of dry selenium-rich Purslane plants crushing and screening qualification in pulverizer, and clean up；Pure clear water, the ethanol solution of 95% concentration, KH2PO4 NaH2PO4 buffer solutions, supersonic generator KH2PO4 NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more, and impregnate 3 hours；By the liquid being obtained by filtration by spraying and dry or vacuum distillation drying is to get to pure natural Purslane plants Organic Selenium.Present invention combination selenium extractive technique and in the environment of Optimal pH 8.5 and additional ultrasound assisted extraction technology significantly improves extraction efficiency to greatly accelerate.
Description
Technical field
The invention belongs to food processing fields, more particularly to one kind high efficiency extraction purslane from Purslane plants
The method of plant selenoprotein.
Background technology
Purslane plants Organic Selenium is the conjugate of selenium and active ingredient in Purslane plants.It is human nutrition selenium source
Basic channel.Even if part is from non-staple food such as animals, from the point of view of food chain, it still comes from Purslane plants selenium and provides
Source.The situation of this resource directly influences the selemium nutrition situation of the mankind.For the first time to the basal nutrient of tall bottle with spout Hairy Bittercress plant at
Divide and carries out network analysis.The result shows that tall bottle with spout Hairy Bittercress plant selenium content is abundant, and moisture, ash content, crude fat, crude protein, diet
Fiber content is considerable, and amino acid classes are complete, is a kind of efficient, excellent natural organic selenium-supply source, is provided for subsequent experimental
Basic data and necessary theoretical direction.Separation and Extraction has been carried out to the different dissolubility protein of tall bottle with spout Hairy Bittercress plant, and
Its antioxidation activity in vitro is investigated.Research shows that most important protein component is aqueous soluble protein matter in tall bottle with spout Hairy Bittercress plant,
The 37.57% of gross protein, followed by alkali-soluble protein matter are accounted for, the 35.75% of gross protein is accounted for.Investigate different dissolubility aleuroplasts
Outer antioxidant activity finds that alkali-soluble protein matter has strongest total antioxidant capacity, Hydroxyl radical-scavenging ability and reducing power, water-soluble
Protein superoxide anion Scavenging activity is most strong.In addition, being determined to the protein binding selenium of different dissolubility protein, send out
Selenium binding protein content highest in existing alkali-soluble protein matter, illustrates that the alkali-soluble protein of tall bottle with spout Hairy Bittercress plant is extraction purification egg containing selenium
White ideal material.The extraction process of tall bottle with spout Hairy Bittercress plant alkali-soluble protein containing selenium has systematically been inquired into for the first time and it has been carried out
Optimization.The factors such as pH, extraction time, Extracting temperature, solid-liquid ratio and extraction time have been investigated to tall bottle with spout Hairy Bittercress plant alkali containing selenium
The influence of molten protein extracting ratio, it is longer to Purslane plants selenium extracting cycle at present.
In view of the above defect existing in the prior art, it is necessary to be further improved, make it more for practicability,
Actual use situation can be met.
Invention content
To overcome above-mentioned deficiency, the present invention to provide a kind of anti-aging graphene nano environment protection modification plastic color master and its system
Standby technique.
The present invention takes following technical scheme to realize：One kind high efficiency extraction Purslane plants from Purslane plants
The method of selenoprotein, includes the following steps：
Step 1：Qualified selenium-rich Purslane plants are screened, and are cleaned up；
Step 2：Clean selenium-rich Purslane plants are dried using the method that drying or the sun are dried；
Step 3：Dry selenium-rich Purslane plants are crushed in pulverizer；
Step 4：Pure clear water is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more, and soak
Bubble 3 hours；
Step 5：The ethanol solution of 95% concentration is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, and impregnate 3 hours；
Step 6：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40
More than, and impregnate 3 hours；
Step 7：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40
More than, it impregnates 3 hours, and container is placed on to the inside of supersonic generator；
Step 8：Feed liquid after stirring is extracted is added in filter device, and liquid is obtained by filtration；
Step 9：By the liquid being obtained by filtration by spraying and dry or vacuum distillation drying is to get to pure natural Purslane plants
Organic Selenium.
Further, the selenium-rich Purslane plants are tall bottle with spout Hairy Bittercress plant.
Further, the step 4 control feed liquid mass ratio is 1:40, it is small that immersion 3 is carried out under 40 DEG C of constant temperature
When, it is filtered after extraction, extraction 3 times is repeated under identical conditions, the filtrate after extraction is merged.
Further, it is volume fraction of ethanol in 95% ethanol solution to concentrate that volume fraction is added in the step 5
It is 80%.
Further, KH2PO4-NaH2PO4 buffer solutions, which are added, in the step 6 makes pH reach 8.5.
Further, the supersonic generator model CQY3-uthe of the step 7.
In summary the invention has the advantages that：The present invention is added by the selenium-rich Purslane plants after crushing
Alkalescent i.e. pH reach 8.5 KH2PO4-NaH2PO4 buffer solutions, and the most important protein component of tall bottle with spout Hairy Bittercress plant is
Aqueous soluble protein matter accounts for the 37.57% of gross protein, followed by alkali-soluble protein matter, accounts for the 35.75% of gross protein, investigates different molten
Solution property protein antioxidation activity in vitro finds that alkali-soluble protein matter has strongest total antioxidant capacity, Hydroxyl radical-scavenging ability
And reducing power, aqueous soluble protein matter superoxide anion Scavenging activity is most strong, in addition, to the protein binding selenium of different dissolubility protein
It is determined, selenium binding protein content highest in discovery alkali-soluble protein matter, pH=8.5, extraction time 3h, 40 DEG C of Extracting temperature,
Solid-liquid ratio 1:40, and under the booster action of supersonic generator, it allows inside sample to be tested and is heated through the medium pair of addition
The absorption of microwave energy accelerates the interaction between sample to be tested molecule and medium molecule to significantly improve extraction efficiency, it can
The shortcomings of to overcome time-consuming, inefficient, consumption reagent in conventional decomposition technique and pollute, ultrasound assisted extraction technology is that having solid examination
It physically or chemically reacts to greatly accelerate by the generation of strong and high-frequency ultrasonic wave in the solution of sample and significantly improves extraction
Efficiency, therefore ultrasonic technique is an auxiliary extraction technology being prioritized compared with conventional extractive technique, is greatly carried
The high speed of extraction Purslane plants selenium.
Specific implementation mode
To make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, with reference to
Specific implementation mode, the present invention is further explained.
Embodiment 1
A method of the high efficiency extraction Purslane plants selenoprotein from Purslane plants, which is characterized in that include the following steps：
（1）：Qualified selenium-rich Purslane plants are screened, and are cleaned up；
（2）：Clean selenium-rich Purslane plants are dried using the method that drying or the sun are dried；
（3）：Dry selenium-rich Purslane plants are crushed in pulverizer；
（4）：Pure clear water is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more, and impregnate 1
Hour；
（5）：The ethanol solution of 95% concentration is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more,
And it impregnates 1 hour；
（6）：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, and impregnate 1 hour；
（7）：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, it impregnates 1 hour, and container is placed on to the inside of supersonic generator；
（8）：Feed liquid after stirring is extracted is added in filter device, and liquid is obtained by filtration；
（9）：The liquid being obtained by filtration is had by spraying and dry or vacuum distillation drying to get to pure natural Purslane plants
Machine selenium.
After testing, ethanol solution, the KH2PO4-NaH2PO4 of the pure clear water, 95% concentration that are used in the present embodiment are slow
Fliud flushing and the KH2PO4-NaH2PO4 buffer solutions extraction Purslane plants selenium indices for having ultrasonic wave to assist are shown in Table 1.
Table 1
| Comparison project | Pure clear water | The ethanol solution of 95% concentration | KH2PO4-NaH2PO4 buffer solutions | The KH2PO4-NaH2PO4 buffer solutions of ultrasonic wave auxiliary |
| Recovery rate（%） | 26.78 | 15.30-16.49 | 30.17 | 60.24 |
In table 1, there is the selenoprotein in the extraction selenium-rich Purslane plants of the KH2PO4-NaH2PO4 buffer solutions of ultrasonic wave auxiliary
Efficiency is apparently higher than pure clear water, the ethanol solution of 95% concentration, KH2PO4-NaH2PO4 buffer solutions.
Embodiment 2
A method of the high efficiency extraction Purslane plants selenoprotein from Purslane plants, which is characterized in that include the following steps：
（1）：Qualified selenium-rich Purslane plants are screened, and are cleaned up；
（2）：Clean selenium-rich Purslane plants are dried using the method that drying or the sun are dried；
（3）：Dry selenium-rich Purslane plants are crushed in pulverizer；
（4）：Pure clear water is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more, and impregnate 1
Hour；
（5）：The ethanol solution of 95% concentration is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more,
And it impregnates 1 hour；
（6）：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, and impregnate 1 hour；
（7）：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, it impregnates 1 hour, and container is placed on to the inside of supersonic generator；
（8）：Feed liquid after stirring is extracted is added in filter device, and liquid is obtained by filtration；
（9）：The liquid being obtained by filtration is had by spraying and dry or vacuum distillation drying to get to pure natural Purslane plants
Machine selenium.
After testing, ethanol solution, the KH2PO4-NaH2PO4 of the pure clear water, 95% concentration that are used in the present embodiment are slow
Fliud flushing and the KH2PO4-NaH2PO4 buffer solutions extraction Purslane plants selenium indices for having ultrasonic wave to assist are shown in Table 1.
Table 2
| Comparison project | Pure clear water | The ethanol solution of 95% concentration | KH2PO4-NaH2PO4 buffer solutions | The KH2PO4-NaH2PO4 buffer solutions of ultrasonic wave auxiliary |
| Recovery rate（%） | 13.14 | 7.70-8.19 | 14.13 | 60.24 |
In table 2, there is the selenoprotein in the extraction selenium-rich Purslane plants of the KH2PO4-NaH2PO4 buffer solutions of ultrasonic wave auxiliary
Efficiency is apparently higher than pure clear water, the ethanol solution of 95% concentration, KH2PO4-NaH2PO4 buffer solutions.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of method of the high efficiency extraction Purslane plants selenoprotein from Purslane plants, which is characterized in that including walking as follows
Suddenly：
Step 1：Qualified selenium-rich Purslane plants are screened, and are cleaned up；
Step 2：Clean selenium-rich Purslane plants are dried using the method that drying or the sun are dried；
Step 3：Dry selenium-rich Purslane plants are crushed in pulverizer；
Step 4：Pure clear water is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 or more, and soak
Bubble 3 hours；
Step 5：The ethanol solution of 95% concentration is added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40 with
On, and impregnate 3 hours；
Step 6：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40
More than, and impregnate 3 hours；
Step 7：KH2PO4-NaH2PO4 buffer solutions are added into the selenium-rich Purslane plants after crushing, control solid-liquid ratio is 1:40
More than, it impregnates 3 hours, and container is placed on to the inside of supersonic generator；
Step 8：Feed liquid after stirring is extracted is added in filter device, and liquid is obtained by filtration；
Step 9：By the liquid being obtained by filtration by spraying and dry or vacuum distillation drying is to get to pure natural Purslane plants
Organic Selenium.
2. a kind of efficiently pure extracting method of rich Purslane plants selenium according to claim 1, it is characterised in that：It is described
Selenium-rich Purslane plants are tall bottle with spout Hairy Bittercress plant.
3. a kind of method of high efficiency extraction Purslane plants selenoprotein from Purslane plants according to claim 1,
It is characterized in that：The step 4 controls feed liquid mass ratio（1:40）, carry out impregnating 3 hours under 40 DEG C of constant temperature, extraction
After filter, under identical conditions repeat extraction 3 times, by after extraction filtrate merge.
4. a kind of method of high efficiency extraction Purslane plants selenoprotein from Purslane plants according to claim 1-3,
It is characterized in that：It is that volume fraction of ethanol is 80% in 95% ethanol solution to concentrate that volume fraction is added in the step 5.
5. a kind of method of high efficiency extraction Purslane plants selenoprotein from Purslane plants according to claim 1-4,
It is characterized in that：KH2PO4-NaH2PO4 buffer solutions, which are added, in the step 6 makes pH reach 8.5.
6. a kind of method of high efficiency extraction Purslane plants selenoprotein from Purslane plants according to claim 1,
It is characterized in that：The supersonic generator model CQY3-uthe of the step 7.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110592167A (en) * | 2019-08-27 | 2019-12-20 | 杨凌萃健生物工程技术有限公司 | Purslane polypeptide extract and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110592167A (en) * | 2019-08-27 | 2019-12-20 | 杨凌萃健生物工程技术有限公司 | Purslane polypeptide extract and preparation method thereof |
| CN110592167B (en) * | 2019-08-27 | 2022-12-23 | 杨凌萃健生物工程技术有限公司 | Purslane polypeptide extract and preparation method thereof |
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Application publication date: 20180713 |