CN108254582A - A kind of microbial respiratory rate apparatus for automatically measuring - Google Patents
A kind of microbial respiratory rate apparatus for automatically measuring Download PDFInfo
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- CN108254582A CN108254582A CN201810071471.XA CN201810071471A CN108254582A CN 108254582 A CN108254582 A CN 108254582A CN 201810071471 A CN201810071471 A CN 201810071471A CN 108254582 A CN108254582 A CN 108254582A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 68
- 230000036387 respiratory rate Effects 0.000 title claims abstract description 54
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 21
- 239000010949 copper Substances 0.000 claims description 21
- 229910052802 copper Inorganic materials 0.000 claims description 21
- 239000002245 particle Substances 0.000 claims description 21
- 238000009423 ventilation Methods 0.000 claims description 18
- 230000000241 respiratory effect Effects 0.000 claims description 16
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 13
- 230000008859 change Effects 0.000 claims description 7
- 230000035945 sensitivity Effects 0.000 claims description 7
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 6
- 239000001569 carbon dioxide Substances 0.000 claims description 6
- 238000011160 research Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 8
- 239000002689 soil Substances 0.000 description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- 229960004424 carbon dioxide Drugs 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 238000001704 evaporation Methods 0.000 description 5
- 230000008020 evaporation Effects 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 241000790917 Dioxys <bee> Species 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003446 memory effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- GCNLQHANGFOQKY-UHFFFAOYSA-N [C+4].[O-2].[O-2].[Ti+4] Chemical compound [C+4].[O-2].[O-2].[Ti+4] GCNLQHANGFOQKY-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000004177 carbon cycle Methods 0.000 description 1
- 229910002090 carbon oxide Inorganic materials 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention provides a kind of microbial respiratory rate apparatus for automatically measuring, is related to agricultural and ecological environment scientific research instrument field.For solving the problems, such as in high volume can not automatically continuously measure microbial respiratory rate in the prior art.Including：At least one culture bottle；Multi-channel control system, including at least one channel, the channel is correspondingly connected with the culture bottle, and the channel is used to obtain the tested gas in the culture bottle；Capnograph is connect with the multi-channel control system, for measuring the gas concentration lwevel data in the tested gas；Data collector is connect with the capnograph, for acquiring the gas concentration lwevel data, and determines microbial respiratory rate according to the gas concentration lwevel data.
Description
Technical field
The present invention relates to agriculturals and ecological environment scientific research instrument field, and in particular to a kind of microbial respiratory rate
Apparatus for automatically measuring.
Background technology
Edaphon respiratory rate data are to reflect the important indicator of soil organic matter decomposable process and microbial activity,
Play important role during ecosystem ecology research and global carbon.In tradition research, it is micro- to obtain soil
The assay method of biological respiratory rate data is all based on the manual of alkali absorption method, infrared spectrum analyser method or gas chromatography etc.
Assay method needs to expend a large amount of manpower and materials in the case where sample size is larger.Based on multiple sample automatic switchover technologies
Apparatus for automatically measuring can well solve these problems.Although having relevant device at present, can only at most realize
The automatic switchover of 16 sample rooms.In this way, in the case where sample size is relatively more, it is desired nonetheless to which frequently replacing sample could complete
It measures, not only arduously, and can cause that environmental factor is very big to the disturbance of sample due to manual switching, influence having for measurement result
Effect property and accuracy.In addition, the price is very expensive for relevant device, its extensive use is also further limited.Therefore, it further opens
Hair can realize that the low-cost equipment that more Multi-example automatically determines just becomes very urgent.
Invention content
The embodiment of the present invention provides a kind of microbial respiratory rate apparatus for automatically measuring, to solve in the prior art can not
The problem of high-volume METHOD FOR CONTINUOUS DETERMINATION microbial respiratory rate.
The embodiment of the present invention provides a kind of microbial respiratory rate apparatus for automatically measuring, including：
At least one culture bottle；
Multi-channel control system, including at least one channel, the channel is correspondingly connected with the culture bottle, the channel
For obtaining the tested gas in the culture bottle；
Capnograph is connect with the multi-channel control system, for measuring the dioxy in the tested gas
Change concentration of carbon data；
Data collector is connect with the capnograph, for acquiring the gas concentration lwevel data, and root
Microbial respiratory rate is determined according to the gas concentration lwevel data.
Preferably, the multi-channel control system includes：
Air inlet electromagnetic valve group, including at least one air inlet electromagnetic valve；The air inlet electromagnetic valve is corresponding with the culture bottle to be connected
Connect, and pass through the first connecting portion part and connect with the capnograph, for by the tested gas from the culture bottle
It is delivered in the capnograph；
Outlet solenoid valve block, including at least one outlet solenoid valve；The outlet solenoid valve is corresponding with the culture bottle to be connected
Connect, and pass through second connecting portion part and connect with the capnograph, for by the tested gas from the titanium dioxide
Carbon analyzer is transmitted back in the culture bottle；And connect by third connecting component with one end of the first air pump, it is big for inciting somebody to action
Gas is delivered in the culture bottle, performs ventilation operation；
At least one multichannel time relay electrically connects respectively with the air inlet electromagnetic valve and the outlet solenoid valve,
For controlling the job order of the air inlet electromagnetic valve and the outlet solenoid valve；
Wherein, the air inlet electromagnetic valve and the outlet solenoid valve being connect with same culture bottle pass through the carbon dioxide
Analyzer intercommunication.
Preferably, the first connecting portion part includes a gas outlet and at least one air inlet；
The second connecting portion part includes an air inlet and at least one gas outlet；
The third connecting component includes an air inlet and at least one gas outlet.
Preferably, the multi-channel control system further includes first filter, one end of the first filter with it is described
The other end connection of first air pump, the other end of the first filter lead directly to air.
Preferably, the multi-channel control system further includes the second air pump, the second filter and gas-guide tube；
The capnograph by the gas-guide tube respectively with the air inlet electromagnetic valve group and the outlet electromagnetism
Valve group connects, and the culture bottle connects by the way that the gas-guide tube is corresponding with the air inlet electromagnetic valve and the outlet solenoid valve respectively
It connects；
The third connecting component is connected by the gas-guide tube with the outlet solenoid valve block and first air pump respectively
It connects；
Second air pump is arranged between the air inlet electromagnetic valve group and the capnograph；
Second filter is arranged between second air pump and the capnograph.
Preferably, water-vapo(u)r saturation gas generating device is further included；The water-vapo(u)r saturation gas generating device includes：Artificial gas
Wait case, copper particle bottle and surge flask；
The copper particle bottle and the surge flask are located in the growth cabinet；The copper particle bottle respectively with first gas
Pump is connected with the surge flask；The surge flask is also connect with the third connecting component；The water-vapo(u)r saturation generation gas dress
It puts the vapour concentration of the air being adjusted to saturation state.
Preferably, the culture bottle is located in the growth cabinet；The growth cabinet is additionally operable to change the training
The temperature of bottle is supported, the temperature sensitivity of microbial respiratory is determined by the microbial respiratory rate measured under different temperatures.
Preferably, T-shaped threeway part is provided in the culture bottle, is connect with the multi-channel control system, for inciting somebody to action
The flow direction of the tested gas is adjusted to horizontal direction.
The embodiment of the present invention provides a kind of microbial respiratory rate apparatus for automatically measuring, including：At least one culture bottle；It is more
Channel control system, including at least one channel, channel is correspondingly connected with culture bottle, and channel is used to obtain tested in culture bottle
Gas；Capnograph is connect with multi-channel control system, for measuring the gas concentration lwevel number in tested gas
According to；Data collector is connect with capnograph, for acquiring gas concentration lwevel data, and it is dense according to carbon dioxide
Degrees of data determines microbial respiratory rate.The embodiment of the present invention, will in the case of high-volume pedotheque continuous automatic measurement
The high-volume pedotheque is respectively put into culture bottle, and passes through the channel of multi-channel control system and each one a pair of culture bottle
It should connect, by the tested gas extracted in each culture bottle and tested gas is delivered to capnograph successively, so
Tested gas is transmitted back in original culture bottle afterwards；Capnograph obtains gas concentration lwevel data according to tested gas,
And the gas concentration lwevel data of acquisition are sent to data collector so that data collector is according to gas concentration lwevel data
Determine microbial respiratory rate；In this way, the embodiment of the present invention can be realized by the channel number of expansion control system it is large quantities of
The pedotheque of amount carries out METHOD FOR CONTINUOUS DETERMINATION, does not need to replace next group culture bottle manually after a collection of culture bottle has been measured, reduce
Due to the interference of acquisition microbial respiratory rate that manual switching is brought, improve microbial respiratory rate determination efficiency and
Accuracy.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the structure diagram of microbial respiratory rate apparatus for automatically measuring provided in an embodiment of the present invention；
Fig. 2 is a kind of second connecting portion part provided in an embodiment of the present invention or the structure diagram of third connecting component；
Fig. 3 is a kind of structure diagram of water-vapo(u)r saturation gas generating device provided in an embodiment of the present invention.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment shall fall within the protection scope of the present invention.
Fig. 1 is the structure diagram of microbial respiratory rate apparatus for automatically measuring provided in an embodiment of the present invention, including：
At least one culture bottle.
Multi-channel control system, including at least one channel, channel is correspondingly connected with culture bottle, and channel is cultivated for obtaining
Tested gas in bottle.
Capnograph is connect with multi-channel control system, for measuring the gas concentration lwevel in tested gas
Data.
Data collector is connect with capnograph, for acquiring gas concentration lwevel data, and according to titanium dioxide
Concentration of carbon data determine microbial respiratory rate.
Wherein, it places pedotheque respectively in each culture bottle, and culture bottle is positioned in isoperibol；Constant temperature ring
The temperature in border is determined by measure personnel.
In practical applications, the channel of the number extension multi-channel control system for the pedotheque that can be measured as needed
Number so that each culture bottle connects one to one respectively with each channel of multi-channel control system；In this way, multi-way contral system
System extracts the tested gas in each culture bottle successively, and then the tested gas of extraction is sent into capnograph, obtains
The gas concentration lwevel data of tested gas different moments are taken, while tested gas is transmitted back in original culture bottle；Carbon dioxide
After analyzer obtains the gas concentration lwevel number of different moments, the gas concentration lwevel data of different moments are sent to data and are adopted
Storage；Data collector receives the gas concentration lwevel data of different moments, and according to the gas concentration lwevel in certain time
Variation determine microbial respiratory rate.
Preferably, multi-channel control system includes：
Air inlet electromagnetic valve group, including at least one air inlet electromagnetic valve；Air inlet electromagnetic valve is correspondingly connected with culture bottle, and is passed through
The first connecting portion part is connect with capnograph, for tested gas to be delivered to capnograph from culture bottle
In.
Outlet solenoid valve block, including at least one outlet solenoid valve；Outlet solenoid valve is correspondingly connected with culture bottle, and is passed through
Second connecting portion part is connect with capnograph, for tested gas to be transmitted back to culture bottle from capnograph
In；And connect by third connecting component with one end of the first air pump, for by Atmospheric Transportation to culture bottle, performing ventilation
Operation.
At least one multichannel time relay, electrically connects respectively with air inlet electromagnetic valve and outlet solenoid valve, for controlling
The job order of air inlet electromagnetic valve and outlet solenoid valve.
Wherein, the air inlet electromagnetic valve and outlet solenoid valve being connect with same culture bottle pass through capnograph intercommunication.
The number of air inlet electromagnetic valve, the number of outlet solenoid valve are identical with the number of culture bottle.
Capnograph includes air inlet and gas outlet.The air inlet of capnograph and air inlet electromagnetic valve group
Connection, the gas outlet of capnograph is connect with outlet solenoid valve block.
Air inlet electromagnetic valve includes air inlet m1, gas outlet m2 and ventilation mouth m3；Air inlet m1 is connect with culture bottle, gas outlet
M2 is connected by the air inlet of the first connecting portion part and capnograph, and ventilation mouth m3 leads directly to air.
Wherein, when air inlet electromagnetic valve is powered, air inlet m1 and gas outlet the m2 conducting of air inlet electromagnetic valve, pair with should be into pneumoelectric
The culture bottle of magnet valve connection carries out microbial respiratory rate determination.When air inlet electromagnetic valve powers off, the air inlet m1 of air inlet electromagnetic valve
With ventilation mouth m3 conductings, take a breath for the culture bottle of air inlet electromagnetic valve connection.
Outlet solenoid valve includes air inlet n1, gas outlet n2 and ventilation mouth n3；Air inlet n1 and capnograph
Gas outlet connects, and gas outlet n2 connect by second connecting portion part with culture bottle, and the mouth n3 that takes a breath passes through third connecting component and the
One air pump one end connects.
Wherein, during outlet solenoid valves, the air inlet n1 and gas outlet n2 of outlet solenoid valve conducting pair go out at this time with this
The culture bottle of pneumoelectric magnet valve connection carries out microbial respiratory rate determination.When outlet solenoid valve powers off, the outlet of outlet solenoid valve
Mouth n2 and the n3 conductings of ventilation mouth, pair culture bottle being connect with the outlet solenoid valve are taken a breath.
In this way, the tested gas in culture bottle is extracted from the air inlet m1 and gas outlet m2 of air inlet electromagnetic valve, by two
After carbonoxide analysis-e/or determining, returned in original culture bottle by the air inlet n1 and gas outlet n2 of outlet solenoid valve.
It should be understood that the air inlet m1 and gas outlet m2 and outlet of the air inlet electromagnetic valve being connect with same culture bottle
The air inlet n1 and gas outlet n2 of solenoid valve form a channel in multi-channel control system；Wherein, connect from different culture bottles
The air inlet electromagnetic valve connect and outlet solenoid valve not intercommunication.Wherein, since the gas concentration lwevel raising in closed environment can press down
Microbial respiratory processed, therefore, in order to improve measure microbial respiratory rate accuracy, outlet solenoid valve when power is off, outlet
Mouth n2 and the n3 conductings of ventilation mouth, when power is off, air inlet m1 and mouth m3 conductings of taking a breath can realize corresponding culture to air inlet electromagnetic valve
The lasting ventilation of bottle.Since the measure of microbial respiratory rate is logical in solenoid valve (including air inlet electromagnetic valve and outlet solenoid valve)
Occur when electric, and take a breath when solenoid valve powers off, thus the measure of microbial respiratory and ventilation are not interfere with each other
's.For synchronization only there are one culture bottle in the measure for carrying out microbial respiratory, other all culture bottles are performing ventilation behaviour
Make.
Based on this, for the ease of controlling the channels operation of multi-channel control system.
One multichannel time relay can be connect with the predetermined number channel of multi-channel control system, control multichannel
The channels operation sequence of control system；The embodiment of the present invention is by the linkage of at least one multichannel time relay, to control
Each channels operation of multi-channel system.Exemplary, microbial respiratory rate apparatus for automatically measuring provided by the invention includes 7
The multichannel time relay, a multichannel time relay include 16 channels；In this manner it is achieved that (7*16=112) is a
The microbial respiratory rate of culture bottle automatically determines.
Specifically, the multichannel time relay is come by setting the sequence of air inlet electromagnetic valve and the sequence of outlet solenoid valve
It is taken a breath to culture bottle and microbial respiratory rate determination.
Wherein, air inlet electromagnetic valve and outlet the solenoid valve sequence being connected on same culture bottle are identical.
The specific embodiment of multichannel time relay control control air inlet electromagnetic valve and outlet electromagnetic valve work includes
Following steps：
S101：The multichannel time relay is powered simultaneously to air inlet electromagnetic valve 1 and outlet solenoid valve 1, and culture bottle 1 is carried out
Microbial respiratory measures, i.e., is pumped to the tested gas in culture bottle 1 in capnograph by air inlet electromagnetic valve 1,
Then it is returned in culture bottle 1 by outlet solenoid valve 1；Meanwhile all culture bottles in addition to culture bottle 1 perform ventilation behaviour
Make.Air inlet electromagnetic valve 1 and outlet solenoid valve 1 are powered off after one measure period.
Wherein, air inlet electromagnetic valve i, the outlet solenoid valve i connections with i-th of culture bottle respectively；I is more than or equal to 1, and i is
Positive integer.
S102：After air inlet electromagnetic valve 1 and outlet solenoid valve 1 power off, the multichannel time relay is to air inlet electromagnetic valve 2 and goes out
Pneumoelectric magnet valve 2 is powered simultaneously, is carried out microbial respiratory measure to culture bottle 2, i.e., the tested gas in culture bottle 2 is passed through air inlet
Solenoid valve 2 is pumped in capnograph, is then returned in culture bottle 2 by outlet solenoid valve 2；Meanwhile except culture
All cultures except bottle 2 perform ventilation operation.And so on until all culture bottles carry out microbial respiratory measure finish.
After all culture bottles measure terminates, when can be by the work period and the beginning that set the multichannel time relay
Between, it automatically begins to the measure of next cycle or the time that next cycle starts is set as needed.
Preferably, the first connecting portion part includes a gas outlet and at least one air inlet；Second connecting portion part includes one
A air inlet and at least one gas outlet；Third connecting component includes an air inlet and at least one gas outlet.
Wherein, the first connecting portion part includes a gas outlet and at least one air inlet；The gas outlet of the first connecting portion part
It is connect with capnograph, the air inlet of the first connecting portion part and the gas outlet m2 of air inlet electromagnetic valve are correspondingly connected with.
Second connecting portion part includes an air inlet and at least one gas outlet；The air inlet and dioxy of second connecting portion part
Change carbon analyzer connection, the gas outlet of second connecting portion part is correspondingly connected with the air inlet n1 of outlet solenoid valve.
Third connecting component includes an air inlet and at least one gas outlet；The air inlet of third connecting component and first
One end connection of air pump, the gas outlet of third connecting component is correspondingly connected with the ventilation mouth n3 of outlet solenoid valve.
It wherein, can be by reducing the volume of the first connecting portion part and the volume of second connecting portion part, to reduce the upper of measure
The error that the memory effect of one culture bottle is brought, i.e., a culture bottle remaining tested gas in multi-channel control system on this
Influence of the body for culture bottle tested at this time, improves the accuracy for measuring microbial respiratory rate.
In practical applications, the first connecting portion part, second connecting portion part and third connecting component are porous device, porous device
Including at least one air inlet and at least one gas outlet, the air inlet of porous device and gas outlet intercommunication, and porous device is each
Intercommunication between air inlet, intercommunication between each gas outlet of porous device.
As shown in Fig. 2, for a kind of second connecting portion part or the structure diagram of third connecting component.Fig. 2 is connected with second
Relay part or third connecting component include what is illustrated for an air inlet a1 and five gas outlets；Wherein, gas outlet is respectively
B1, b2, b3, b4 and b5.
Exemplary, the structure of the first connecting portion part is identical with second connecting portion part shown in Fig. 2 or third connecting component, figure
Five gas outlet b1, b2, b3, b4 and b5 in 2 respectively can be as the air inlet of the first connecting portion part, the air inlet in Fig. 2
A1 can be as the gas outlet of the first connecting portion part.
It, can be by reducing the volume of porous device, that is to say, that reduce the gas outlet and second of the first connecting portion part based on this
The length of the air inlet of connecting component, to reduce the influence of memory effect.
Exemplary, porous device includes an air inlet and 112 gas outlets；The internal capacity of the porous device is 1.5ml, outside
A diameter of 35mm, a length of 120mm, internal diameter 4mm；112 gas outlets are connected with the internal diameter of 4mm.
Further, the length of the gas-guide tube between porous device and capnograph can also be reduced, on reducing
Tested gas in one culture bottle is for the influence of the culture bottle measured.
Preferably, multi-channel control system further includes first filter, and one end of first filter is another with the first air pump
One end connects, and the other end of first filter leads directly to air；It in this way, can be with the solid in filtered atmospheric by first filter
Grain reduces damage of the solid particle in air for microbial respiratory rate apparatus for automatically measuring provided in an embodiment of the present invention
Evil.
For the ease of the tested gas in culture bottle is pumped into capnograph, and improve measure microbial respiratory
The accuracy of rate, it is preferable that multi-channel control system further includes the second air pump, the second filter and gas-guide tube.
Capnograph is connect, and culture bottle by gas-guide tube with air inlet electromagnetic valve group and outlet solenoid valve block respectively
It is correspondingly connected with respectively with air inlet electromagnetic valve and outlet solenoid valve by gas-guide tube.
Third connecting component is connect by gas-guide tube with outlet solenoid valve block and the first air pump respectively.
Second air pump is arranged between air inlet electromagnetic valve group and capnograph.
Second filter is arranged between the second air pump and capnograph, for reducing the solid in culture bottle
Damage of the grain for microbial respiratory rate apparatus for automatically measuring provided in an embodiment of the present invention, and improve measure microbial respiratory
The accuracy of rate.Further, since the evaporation of soil moisture in culture bottle can be accelerated by taking a breath to culture bottle, in order to inhibit to train
Support the pedotheque moisture evaporation in bottle, it is preferable that further include water-vapo(u)r saturation gas generating device；Water-vapo(u)r saturation gas generation dress
Put including：Growth cabinet, copper particle bottle and surge flask；
Copper particle bottle and surge flask are located in growth cabinet；Copper particle bottle is connect respectively with the first air pump and surge flask；Buffering
Bottle is also connect with third connecting component；Water-vapo(u)r saturation generation gas device is used to the vapour concentration of air being adjusted to saturation shape
State.Specifically as shown in figure 3, for a kind of structure diagram of water-vapo(u)r saturation gas generating device provided in an embodiment of the present invention.
Wherein, the temperature of climate box is predeterminable, and the temperature of climate box is the cultivation temperature of microbial respiratory；In copper particle bottle
Including copper particle and a small amount of pure water.
In practical applications, surge flask is connected by third connecting component and the outlet solenoid valve block of multi-channel control system
Connect, come in the water-vapo(u)r saturation Atmospheric Transportation to culture bottle that is generated water-vapo(u)r saturation generating means by the first air pump, and by into
The ventilation mouth m3 of pneumoelectric magnet valve is emitted into outside culture bottle.
Since copper thermal conductivity is very big and specific heat capacity is very low, during climate box alternating temperature, copper particle temperature can be quickly with gas
The temperature for waiting case changes and changes so that the temperature of copper particle bottle is identical with the temperature of climate box.
In this way, when if weather the temperature inside the box is less than the atmospheric temperature for being pumped into copper particle bottle, then, the air into copper particle bottle is met
Copper particle to low temperature occurs to condense and generate the water-vapo(u)r saturation air under climate box low temperature.If artificial climate the temperature inside the box is higher than pumping
When entering the atmospheric temperature of copper particle bottle, then, into climate box air by that can be dramatically increased after the mixture of copper particle bottle and water
The vapour concentration of the air of copper particle bottle generates the water-vapo(u)r saturation air under climate box high temperature until close to saturation state；Then
Multi-channel control system is entered, and then to taking a breath in culture bottle, prevent the water in water-vapo(u)r saturation air by surge flask
Divide and the moisture in copper particle bottle is brought into multi-channel control system, reduce the mistake of acquisition microbial respiratory rate that steam is brought
Difference.In this way, gas concentration lwevel is normal in maintenance culture bottle, significantly reduces soil evaporation and soil is dried
Speed.
Preferably, culture bottle is located in growth cabinet；Growth cabinet is additionally operable to change the temperature of culture bottle, passes through survey
Determine the temperature sensitivity that the microbial respiratory rate under different temperatures determines microbial respiratory.
In practical applications, micro- life under different temperatures can be measured by setting the automatic temperature changing program of growth cabinet
Object respiratory rate determines the temperature sensitivity of microbial respiratory.
Wherein, temperature sensitivity refer to microbial respiratory to temperature raised response index；The temperature of edaphon breathing
Degree sensibility plays very crucial effect in global carbon simulates and predict that following global warming influences carbon cycle.
It, can be by continuously adjusting the temperature of the climate box residing for culture bottle, the at different temperatures each training of METHOD FOR CONTINUOUS DETERMINATION in practical application
The microbial respiratory rate of bottle is supported, and then determines the temperature sensitivity of microorganism, i.e., between temperature and microbial respiratory rate
Relationship.
Preferably, T-shaped threeway part is provided in culture bottle, is connect with multi-channel control system, for by tested gas
Flow direction be adjusted to horizontal direction.
Wherein, T-shaped threeway part includes three blow vents, respectively blow vent 1, blow vent 2 and blow vent 3.Blow vent 2
It is located at same horizontal line with blow vent 3, blow vent 1 is perpendicular to blow vent 2 and blow vent 3.In practical applications, blow vent 1 is logical
Gas outlet of the gas-guide tube respectively with the air inlet of air inlet electromagnetic valve and outlet solenoid valve is crossed to connect.
In this way, the direction that outlet solenoid valve is parallel to soil surface to the tested gas that culture bottle is sent back to enters culture bottle,
Soil is blowed to without facing directly, so as to reduce the evaporation of culture bottle soil surface.
Further, microbial respiratory rate apparatus for automatically measuring provided in an embodiment of the present invention further includes power supply module,
It is electrically connected respectively with multi-channel control system and capnograph.Power supply module is used for capnograph and Duo Tong
Road control system power supply；Electric power system electrically connects respectively with capnograph and multi-channel control system.Need what is illustrated
Be, the embodiment of the present invention for electric power system power supply mode without limit.
It should be noted that the embodiment of the present invention for first filter and the second filter aperture without limit.
Exemplary, the aperture of first filter and the second filter is respectively between 0.2-1 microns.Flow velocity for the first air pump and
The flow velocity of two air pumps is without limiting.Exemplary, the flow velocity of the first air pump is between 1L/min, and the flow velocity of the second air pump is according to logical
Road quantity determines, ensures the flow velocity of average each channel in 0.5L/min or so.
The embodiment of the present invention is in the case of high-volume pedotheque continuous automatic measurement, by the high-volume pedotheque point
It is not put into culture bottle, and passes through the channel of multi-channel control system and each culture bottle connects one to one, to take out successively
It takes the tested gas in each culture bottle and tested gas is delivered to capnograph, be then transmitted back to tested gas
In original culture bottle；Capnograph obtains gas concentration lwevel data according to tested gas, and by the carbon dioxide of acquisition
Concentration data is sent to data collector so that data collector determines microbial respiratory speed according to gas concentration lwevel data
Rate；In this way, the embodiment of the present invention can realize that large batch of pedotheque is carried out by the channel number of expansion control system
METHOD FOR CONTINUOUS DETERMINATION is not needed to replace next group culture bottle manually after a collection of culture bottle has been measured, be reduced due to manual switching band
The interference for the acquisition microbial respiratory rate come improves the efficiency and accuracy of microbial respiratory rate determination.
Further, air inlet electromagnetic valve provided by the invention further includes ventilation mouth m3, and outlet solenoid valve further includes ventilation mouth
N3, for before microbial respiratory rate determination is carried out to culture bottle, taking a breath to culture product so that the dioxy of tested gas
Change concentration of carbon is identical with atmospheric carbon dioxide concentration, improves the accuracy for measuring microbial respiratory rate.Further, this hair
The microbial respiratory rate apparatus for automatically measuring of bright offer further includes water-vapo(u)r saturation generating means, culture bottle to be inhibited to take a breath
Caused soil water evaporation in the process.
Further, the automatic temperature changing program of the invention by setting growth cabinet, can be by measuring different temperatures
Under microbial respiratory rate determine the temperature sensitivity of microbial respiratory.
Although preferred embodiments of the present invention have been described, but those skilled in the art once know basic creation
Property concept, then additional changes and modifications may be made to these embodiments.So appended claims be intended to be construed to include it is excellent
It selects embodiment and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (8)
1. a kind of microbial respiratory rate apparatus for automatically measuring, which is characterized in that including：
At least one culture bottle；
Multi-channel control system, including at least one channel, the channel is correspondingly connected with the culture bottle, and the channel is used for
Obtain the tested gas in the culture bottle；
Capnograph is connect with the multi-channel control system, for measuring the carbon dioxide in the tested gas
Concentration data；
Data collector is connect with the capnograph, for acquiring the gas concentration lwevel data, and according to institute
It states gas concentration lwevel data and determines microbial respiratory rate.
2. microbial respiratory rate apparatus for automatically measuring according to claim 1, which is characterized in that the multi-way contral
System includes：
Air inlet electromagnetic valve group, including at least one air inlet electromagnetic valve；The air inlet electromagnetic valve is correspondingly connected with the culture bottle, and
It is connect by the first connecting portion part with the capnograph, for the tested gas to be delivered to from the culture bottle
In the capnograph；
Outlet solenoid valve block, including at least one outlet solenoid valve；The outlet solenoid valve is correspondingly connected with the culture bottle, and
Connect by second connecting portion part with the capnograph, for by the tested gas from the carboloy dioxide analysis
Instrument is transmitted back in the culture bottle；And connect by third connecting component with one end of the first air pump, for by Atmospheric Transportation
Into the culture bottle, ventilation operation is performed；
At least one multichannel time relay is electrically connected with the air inlet electromagnetic valve and the outlet solenoid valve, is used for respectively
Control the job order of the air inlet electromagnetic valve and the outlet solenoid valve；
Wherein, the air inlet electromagnetic valve and the outlet solenoid valve being connect with same culture bottle pass through the carboloy dioxide analysis
Instrument intercommunication.
3. microbial respiratory rate apparatus for automatically measuring according to claim 2, which is characterized in that
The first connecting portion part includes a gas outlet and at least one air inlet；
The second connecting portion part includes an air inlet and at least one gas outlet；
The third connecting component includes an air inlet and at least one gas outlet.
4. microbial respiratory rate apparatus for automatically measuring according to claim 2, which is characterized in that the multi-way contral
System further includes first filter, and one end of the first filter is connect with the other end of first air pump, and described first
The other end of filter leads directly to air.
5. microbial respiratory rate apparatus for automatically measuring according to claim 2, which is characterized in that the multi-way contral
System further includes the second air pump, the second filter and gas-guide tube；
The capnograph by the gas-guide tube respectively with the air inlet electromagnetic valve group and the outlet solenoid valve block
Connection, and the culture bottle is correspondingly connected with by the gas-guide tube with the air inlet electromagnetic valve and the outlet solenoid valve respectively；
The third connecting component is connect by the gas-guide tube with the outlet solenoid valve block and first air pump respectively；
Second air pump is arranged between the air inlet electromagnetic valve group and the capnograph；
Second filter is arranged between second air pump and the capnograph.
6. microbial respiratory rate apparatus for automatically measuring according to claim 2, which is characterized in that further include water-vapo(u)r saturation
Gas generating device；The water-vapo(u)r saturation gas generating device includes：Growth cabinet, copper particle bottle and surge flask；
The copper particle bottle and the surge flask are located in the growth cabinet；The copper particle bottle respectively with first air pump and
The surge flask connection；The surge flask is also connect with the third connecting component；The water-vapo(u)r saturation generation gas device is used
In the vapour concentration of the air is adjusted to saturation state.
7. microbial respiratory rate apparatus for automatically measuring according to claim 6, which is characterized in that the culture bottle is located at
In the growth cabinet；The growth cabinet is additionally operable to change the temperature of the culture bottle, by measuring under different temperatures
Microbial respiratory rate determine the temperature sensitivity of microbial respiratory.
8. according to claim 1~7 any one of them microbial respiratory rate apparatus for automatically measuring, which is characterized in that described
T-shaped threeway part is provided in culture bottle, is connect with the multi-channel control system, for by the flow direction tune of the tested gas
It saves as horizontal direction.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208562A (en) * | 2019-06-24 | 2019-09-06 | 中国科学院南京地理与湖泊研究所 | Greenhouse gases automatic sample handling system |
| CN111239362A (en) * | 2019-09-26 | 2020-06-05 | 浙江农林大学 | Soil respiration monitoring system and method |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09135679A (en) * | 1995-11-14 | 1997-05-27 | Kurita Water Ind Ltd | Apparatus for measuring respiration rate of microorganism |
| US20050135969A1 (en) * | 1996-11-06 | 2005-06-23 | Olsson Sven G. | Method and apparatus for determining indirectly the concentration of a specific substance in the blood |
| CN102053145A (en) * | 2010-11-03 | 2011-05-11 | 中国科学院新疆生态与地理研究所 | In-situ automatic measurement method of CO2 of root system |
| CN102586097A (en) * | 2012-01-12 | 2012-07-18 | 中国科学院地理科学与资源研究所 | Device of continuously testing indoor soil microbial respiration |
| CN103760304A (en) * | 2014-01-08 | 2014-04-30 | 吉林省农业科学院 | Multifunctional gas analyzer for livestock |
| CN106442066A (en) * | 2016-11-09 | 2017-02-22 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small air collector |
| CN107389586A (en) * | 2017-09-04 | 2017-11-24 | 中国科学院地理科学与资源研究所 | δ13C METHOD FOR CONTINUOUS DETERMINATION systems |
| CN208013237U (en) * | 2018-01-25 | 2018-10-26 | 中山大学 | A kind of microbial respiratory rate apparatus for automatically measuring |
-
2018
- 2018-01-25 CN CN201810071471.XA patent/CN108254582A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09135679A (en) * | 1995-11-14 | 1997-05-27 | Kurita Water Ind Ltd | Apparatus for measuring respiration rate of microorganism |
| US20050135969A1 (en) * | 1996-11-06 | 2005-06-23 | Olsson Sven G. | Method and apparatus for determining indirectly the concentration of a specific substance in the blood |
| CN102053145A (en) * | 2010-11-03 | 2011-05-11 | 中国科学院新疆生态与地理研究所 | In-situ automatic measurement method of CO2 of root system |
| CN102586097A (en) * | 2012-01-12 | 2012-07-18 | 中国科学院地理科学与资源研究所 | Device of continuously testing indoor soil microbial respiration |
| CN103760304A (en) * | 2014-01-08 | 2014-04-30 | 吉林省农业科学院 | Multifunctional gas analyzer for livestock |
| CN106442066A (en) * | 2016-11-09 | 2017-02-22 | 中国农业科学院农业资源与农业区划研究所 | Soil culture device with small air collector |
| CN107389586A (en) * | 2017-09-04 | 2017-11-24 | 中国科学院地理科学与资源研究所 | δ13C METHOD FOR CONTINUOUS DETERMINATION systems |
| CN208013237U (en) * | 2018-01-25 | 2018-10-26 | 中山大学 | A kind of microbial respiratory rate apparatus for automatically measuring |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208562A (en) * | 2019-06-24 | 2019-09-06 | 中国科学院南京地理与湖泊研究所 | Greenhouse gases automatic sample handling system |
| CN111239362A (en) * | 2019-09-26 | 2020-06-05 | 浙江农林大学 | Soil respiration monitoring system and method |
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