CN108254573A - The computational methods and equipment of intrathecal IgA, assay method and computer readable storage medium - Google Patents

The computational methods and equipment of intrathecal IgA, assay method and computer readable storage medium Download PDF

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CN108254573A
CN108254573A CN201810006469.4A CN201810006469A CN108254573A CN 108254573 A CN108254573 A CN 108254573A CN 201810006469 A CN201810006469 A CN 201810006469A CN 108254573 A CN108254573 A CN 108254573A
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iga
alb
lim
intrathecal
concentration
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宁紫妍
李超
朱峰
王赫群
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Shenzhen Central South Medical Laboratory Laboratory
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Abstract

The present invention discloses a kind of assay method of intrathecal IgA, including：Obtain concentration [IgAs of the IgA in cerebrospinal fluid in sample1], concentration [IgAs of the IgA in serum2], concentration [AlbBs of the Alb in cerebrospinal fluid1] and concentration [AlbBs of the Alb in serum2]；Compare QIgAWith QLim(IgA)Size, wherein, QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2], if QIgA＞ QLim(IgA), then intrathecal IgA generation concentration：IgIF ACSF=[IgA1]×[1‑QLim(IgA)/QIgA].Technical solution of the present invention improves the precision of the measure of intrathecal IgA.

Description

The computational methods and equipment of intrathecal IgA, assay method and computer readable storage medium
Technical field
The present invention relates to immune protein detection technique field, the computational methods and equipment of more particularly to a kind of intrathecal IgA, Assay method and computer readable storage medium.
Background technology
Very strong intrathecal immune response can be generated in central nervous system, this be it is most of infectious and it is certain itself Immunity the nervous system disease occurs, the pathological basis of development.
Therefore cerebrospinal fluid (cerebrospinal fluid, CSF) examine, particularly wherein immunoglobulin ingredient and its The detection of content is of great significance to the diagnosis of certain central nervous system diseases, observation of curative effect and Index for diagnosis.
Many the nervous system diseases are along with typical intrathecal immune response, these cerebrospinal fluid immunoglobulin sheaths The quantity of interior generation and change of illness state are closely related, thus accurate quantification is needed to analyze the truth that can just obtain patient.Brain Many protein in spinal fluid, such as albumin and immunoglobulin, are mainly derived from except brain, are by blood brain barrier It penetrates into cerebrospinal fluid, therefore their concentration levels in cerebrospinal fluid are inevitably by same time serum-concentration Horizontal influence.And current domestic cerebrospinal fluid immunology detection only detects containing for a certain immunoglobulin like protein in cerebrospinal fluid Cerebrospinal fluid immunoglobulin concentration is simply equal to intrathecal immunoglobulin generation level, had ignored of the same race in serum by amount The influence of protein concentration levels and blood brain barrier function to concentration level in its cerebrospinal fluid, causes its clinical meaning to be limited, no Intrathecal inflammatory reaction whether cannot only occur for patient due diagnosis basis is provided or even can also mislead sentencing for clinician It is disconnected.Therefore, urgently one kind being capable of the intrathecal life of certain immunoglobulin of accurate evaluation in the immune diagnostic technique of the nervous system disease Into horizontal and its proportional amount of analysis method.
Invention content
The main object of the present invention is to provide a kind of assay method of intrathecal IgA, it is intended to improve the essence of intrathecal IgA detections Accuracy.
To achieve the above object, the present invention provides a kind of assay method of intrathecal IgA, including：
Obtain concentration [IgAs of the IgA in cerebrospinal fluid in sample1], concentration [IgAs of the IgA in serum2], Alb is in brain ridge Concentration [AlbB in liquid1] and concentration [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein,
QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2],
If QIgA＞ QLim(IgA), then intrathecal IgA generation concentration：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
Preferably, QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
Preferably, if QIgA≤QLim(IgA), then intrathecal IgA generation concentration：
IgIF ACSF=0.
The present invention also provides a kind of density calculating method of intrathecal IgA, including：
Receive concentration value [IgAs of the IgA of input in cerebrospinal fluid1], concentration value [IgAs of the IgA in serum2], Alb exists Concentration value [AlbB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein,
QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb),
QAlb=[AlbB1]/[AlbB2]；
If QIgA＞ QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
Preferably, QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
Preferably, if QIgA≤QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=0.
The present invention also provides a kind of intrathecal IgA sensing equipments, including：
Receiving module, concentration value [IgAs of the IgA in cerebrospinal fluid measured to real-time reception1], IgA is in serum Concentration value [IgA2], concentration value [AlbBs of the AlB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Computing module, to calculate QLim(IgA)Value and QAlbValue, wherein,
QIgA=[IgA1]/[IgA2], QAlb=[AlbB1]/[AlbB2],
QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2]；
Contrast module, to compare QLim(IgA)Value and QAlbThe size of value；
Output module, to work as QIgA＞ QLim(IgA)When, export the concentration value IgIF A of intrathecal IgACSF=[IgA1]×[1- QLim(IgA)/QIgA]。
Preferably, QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
Preferably, the output module is also working as QIgA≤QLim(IgA), export the concentration value of intrathecal IgA： IgIF ACSF =0.
The present invention also provides a kind of computer readable storage medium, the computer-readable recording medium storage has data Processing routine when the data processor is executed by processor, realizes following steps：
Receive concentration value [IgAs of the IgA of input in cerebrospinal fluid1], concentration value [IgAs of the IgA in serum2], Alb exists Concentration value [AlbB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein,
QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb),
QAlb=[AlbB1]/[AlbB2]；
If QIgA＞ QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
Due to the presence of blood brain barrier, protein molecule is from the blood of high concentration gradient by the filtration of selectivity to low In the cerebrospinal fluid of concentration gradient.Technical solution of the present invention is weak according to the big protein molecular of molecular weight " penetrability ", and molecular weight is small Protein molecular " penetrability " by force, passes through quotient QIgATo represent the power of this " penetrability ", and establish normal human QLim(IgA)With QAlbExisting relational expression：QLim(IgA)=a [(QAlb)2+b2)]1/2- c works as QIgA＞ QLim(IgA)When, obtain intrathecal IgA Generation concentration：IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical solution in the embodiment of the present invention is clearly and completely retouched It states, it is clear that described embodiment is only the part of the embodiment of the present invention, instead of all the embodiments.Based on this hair Embodiment in bright, the every other reality that those of ordinary skill in the art are obtained without creative efforts Example is applied, shall fall within the protection scope of the present invention.
It is of the invention to propose a kind of assay method of intrathecal IgA, the computational methods of intrathecal IgA and its equipment in fact, be stored with The computer readable storage medium of the computational methods of intrathecal IgA.
Due to the presence of blood brain barrier, protein molecule is from the blood of high concentration gradient by the filtration of selectivity to low In the cerebrospinal fluid of concentration gradient, the big protein molecular of molecular weight " penetrability " is weak, the small protein molecular of molecular weight " penetrability " By force.The technology of the present invention takes immunoglobulin quotient QIgATo represent the power of this " penetrability ".Immunoglobulin A quotient Ratio of the computational methods between the concentration of immunoglobulin A in the concentration of immunoglobulin A in cerebrospinal fluid and same time serum Value, calculation formula are as follows： QIgA=[IgA1]/[IgA2], QIgAIt is bigger, show that " penetrability " is stronger.
To verify QIgAWith QAlbBetween relationship, take the cerebrospinal fluid and serum of first 35 people, measure brain ridge respectively Q in liquidIgAWith QAlbValue.
Sample M1
Same time takes sample M1 cerebrospinal fluid and peripheral blood sample using the mode of lumbar puncture and venous blood collection respectively.
Using immunoturbidimetry manually or automatically biochemical instruments to the albumin in sample M1 cerebrospinal fluid and serum sample (Alb) and immunoglobulin IgA content carries out quantitative experiment analysis.
Experimental result shows that sample M1 cerebrospinal fluid albumin concentration is 836mg/l, seralbumin concentration 36.3g/ L, cerebrospinal fluid immunoglobulin IgA are 48.8mg/l, and serum immune globulin IgA is 3.25g/l.It is computed, the white eggs of sample M1 White quotient is 23 × 10-3, Immunoglobulin IgA quotient is respectively 15 × 10-3。
Sample M2
Same time takes sample M1 cerebrospinal fluid and peripheral blood sample using the mode of lumbar puncture and venous blood collection respectively.
Using immunoturbidimetry manually or automatically biochemical instruments to the albumin in sample M2 cerebrospinal fluid and serum sample (Alb) and immunoglobulin IgA content carries out quantitative experiment analysis.
Experimental result shows that sample M2 cerebrospinal fluid albumin concentration is 1189mg/l, and seralbumin concentration is 41.57g/l, cerebrospinal fluid immunoglobulin IgA 20.8mg/l, serum immune globulin IgA are 1.08g/l.It is computed, sample M2 albumin quotient is 28.6 × 10-3, Immunoglobulin IgA quotient is respectively 19.2 × 10-3。
Sample M3
Same time takes sample M3 cerebrospinal fluid and peripheral blood sample using the mode of lumbar puncture and venous blood collection respectively.
Using immunoturbidimetry manually or automatically biochemical instruments to the albumin in sample M3 cerebrospinal fluid and serum sample (Alb) and immunoglobulin IgA content carries out quantitative experiment analysis.
Experimental result shows that sample M3 cerebrospinal fluid albumin concentration is 198mg/l, seralbumin concentration 34.1g/ L, cerebrospinal fluid immunoglobulin IgA 2.88mg/l, serum immune globulin IgA are 1.07g/l.It is computed, the white eggs of sample M3 White quotient is 5.8, and Immunoglobulin IgA quotient is respectively 2.69 × 10-3。
It can be found that Q from tableIgAWith QAlbIncrease and increase, QIgAWith QAlbPositive correlation, that is, QLim(IgA)=f (QAlb)。
(1), linearly related guess
Linear fit is carried out to above-mentioned 35 data using fitting software, obtains fitting formula：
QIgA=1.308QAlb+ 3.159, (R2=0.999)；
Linear fit is carried out to the data of above-mentioned M3-M11 using fitting software, obtains fitting formula：
QIgA=1.615QAlb+ 1.302, (R2=0.995)；
Linear fit is carried out to the data of above-mentioned M12-M22 using fitting software, obtains fitting formula：
QIgA=1.314QAlb+ 3.291, (R2=0.999)；
Linear fit is carried out to the data of above-mentioned M28-M35 using fitting software, obtains fitting formula：
QIgA=1.304QAlb+ 3.538, (R2=0.999)；
For QAlbValue is bigger, and the slope of fitting formula tends to 1.3, and works as QAlbWhen being worth less than normal, slope variation compared with Greatly, and correlation is not achieved 0.999, so, above-mentioned fitting formula poor accuracy.
It can be seen that for QIgAWith QAlbCorrelation, be intended to curvilinear correlation.
(2), power exponent correlation is guessed
Power exponent fitting is carried out to above-mentioned 35 data using fitting software, obtains fitting formula：
Obviously, this correlation is not achieved 0.999, so, power exponent curvilinear correlation is also not accurate enough.
(3), multinomial correlation is guessed
Fitting of a polynomial is carried out to above-mentioned 35 data using fitting software, obtains fitting formula：
Fitting of a polynomial is carried out to above-mentioned M3-M11 using fitting software, obtains fitting formula：
It can be seen that for partial data QAlbFor relatively low data, QIgAWith QAlbCorrelation intend with 38 data The correlation closed out has difference.
(4), other correlations are guessed
Above-mentioned 35 data are fitted using fitting software, obtain fitting formula：
QIgA=0.77 [(QAlb)2+(4.79×10-3)2]1/2-3.1×10-3, (R2=1)
Above-mentioned M3-M11 is fitted using fitting software, obtains fitting formula：
QIgA=0.75 [(QAlb)2+(4.8×10-3)2]1/2-3.3×10-3, (R2=1)；
Above-mentioned M12-M22 is fitted using fitting software, obtains fitting formula：
QIgA=0.8 [(QAlb)2+(4.5×10-3)2]1/2-3.2×10-3, (R2=1)；
Linear fit is carried out to the data of above-mentioned M28-M35 using fitting software, obtains fitting formula：
QIgA=0.78 [(QA1b)2+(4.8×10-3)2]1/2-3.0×10-3, (R2=1).
It can be seen that the formula of fitting：QIgA=a [(QAlb)2+b2)]1/2- c, wherein a ∈ [0.75,0.8], b ∈ [4.5 ×10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3], there is fabulous correlation.
(5), it verifies
Since the quantity of above-mentioned fitting data is 35 groups, may not have generality also, for this purpose, with formula QIgA=0.77 [(QA1b)2+(4.79×10-3)2]1/2-3.1×10-3To verify the accuracy of fitting.
As can be seen from the above table, go out outside Q7 data, in other 22 groups of data,Measured value and calculated value With fabulous consistency.There is relatively large deviation in this Q7 data, it may be possible to sample be contaminated either test errors or The reasons such as reagent is expired.
It can to sum up obtain, normal person's (without illness, intrathecal without generation IgA), internal QIgAWith QAlbThere are fabulous Correlation.That is to say, immunoglobulin quotient at this time be immunoglobulin A it is intrathecal without generation with reference to boundary value QLim(IgA)：
QLim(IgA)=a [(QAlb)2+b2)]1/2- c, wherein a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈[3.0×10-3, 3.2 × 10-3]。
If measure human body QIgAHigher than QLim(IgA), then illustrate the human body there are intrathecal IgA to generate, so as to calculate Go out the concentration value of intrathecal IgA：IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA], [IgA1] dense in cerebrospinal fluid for IgA Degree, [IgA2] for concentration of the IgA in serum, QIgA=[IgA1]/[IgA2]；
Otherwise the concentration value of intrathecal IgA is generated namely exported without intrathecal IgA：IgIF ACSF=0.
Analysis of cases：
Case 1, by taking the cerebrospinal fluid detection of neural tuberculosis patient as an example, the detection assessed its intrathecal antibody tormation walks It is rapid as follows：
Same time takes Cerebrospinal Fluid in Patients and peripheral blood sample this application using the mode of lumbar puncture and venous blood collection respectively Immunoturbidimetry manually or automatically biochemical instruments to the albumin in Cerebrospinal Fluid in Patients and serum sample and Immunoglobulin IgA/M/ G contents carry out quantitative experiment analysis.
Experimental result shows, Cerebrospinal Fluid in Patients albumin concentration is 809mg/l, seralbumin concentration 35.2g/l, Cerebrospinal fluid immunoglobulin IgA/M/G is respectively 55.7mg/l, 31.4mg/l, 189mg/l, serum immune globulin IgA/M/G Respectively 2.9g/l, 5.9g/l, 13.6g/l.It is computed, patient's albumin quotient is 23, Immunoglobulin IgA/M/G quotients Respectively 19.2,5.3,13.9.And it is intrathecal without generation ginseng to extrapolate Immunoglobulin IgA/M/G according to patient's albumin quotient It is respectively 15,10,19.8 to examine boundary value.
Analysis result shows that the raising of Immunoglobulin IgM and IgG cerebrospinal fluidconcentrations is because of blood brain barrier permeability Increase lead to that haematogenous antibody increases as a result, the not intrathecal generation of center of origin nervous system endogenous.Only IgA is true Theoretical reference value is had exceeded, intrathecal generation has occurred, clinician is lungy to patients' nerve examines for strong supporting It is disconnected.And according to domestic existing cerebrospinal fluid immunoglobulin reference standard (IgA0-6mg/L, IgM 0-13mg/L, IgG 10- 40mg/L), three indexs of patient's immunoglobulin are much exceeded, and significantly intrathecal generation occurs, should be diagnosed as suppurating Property meningitis or subarachnoid hemorrhage, deviate from last diagnostic result.
Case 2, the CSF sample derive from a patients with virus encephalitis, the inspection assessed its intrathecal antibody tormation It is as follows to survey step：
Same time takes Cerebrospinal Fluid in Patients and peripheral blood sample this application using the mode of lumbar puncture and venous blood collection respectively Immunoturbidimetry manually or automatically biochemical instruments to the albumin in Cerebrospinal Fluid in Patients and serum sample and Immunoglobulin IgA/M/ G contents carry out quantitative experiment analysis.
Experimental result shows, Cerebrospinal Fluid in Patients albumin concentration is 1350mg/l, seralbumin concentration 47.2g/l, Cerebrospinal fluid immunoglobulin IgA/M/G is respectively 22.2mg/l, 13.3mg/l, 320mg/l, serum immune globulin IgA/M/G Respectively 1.5g/l, 1.3g/l, 9.6g/l.It is computed, patient's albumin quotient is 28.6, Immunoglobulin IgA/M/G quotients Respectively 14.5,10.2,33.3.And it is intrathecal without generation ginseng to extrapolate Immunoglobulin IgA/M/G according to patient's albumin quotient It is respectively 19,13,25 to examine boundary value.
Analysis result shows that Immunoglobulin IgG quotient is higher than reference value, shows center of origin nervous system endogenous Intrathecal generation, and other immunoglobulin quotients illustrate in reference range without intrathecal generation.Coordinate cerebrospinal fluid cytology It counts and lactate level measurement result, all indexs is directed toward viral encephalitis.And according to domestic existing cerebrospinal fluid immunoglobulin Reference standard (IgA 0-6mg/L, IgM 0-13mg/L, IgG 10-40mg/L), three indexs of patient's immunoglobulin are remote It is remote exceeded, significantly intrathecal generation occurs, purulent meningitis or subarachnoid hemorrhage should be diagnosed as, and cerebrospinal fluid is thin Born of the same parents' count results show that purulence feature does not occur in the patient, also do not find that the instructions such as red blood cell and transferrins are intracranialed hemorrhage Index and immunoglobulin analysis result it is conflicting, cause to puzzle to clinician.
Case 3, by taking the cerebrospinal fluid detection of multiple sclerosis patients as an example, the detection to its intrathecal antibody tormation assessment Step is as follows：
Same time takes Cerebrospinal Fluid in Patients and peripheral blood sample this application using the mode of lumbar puncture and venous blood collection respectively Immunoturbidimetry manually or automatically biochemical instruments to the albumin in Cerebrospinal Fluid in Patients and serum sample and Immunoglobulin IgA/M/ G contents carry out quantitative experiment analysis.
Experimental result shows, Cerebrospinal Fluid in Patients albumin concentration is 214mg/l, seralbumin concentration 37.1g/l, Cerebrospinal fluid immunoglobulin IgA/M/G is respectively 3.6mg/l, 0.95mg/l, 31.7mg/l, serum immune globulin IgA/M/G Respectively 2.1g/l, 2.5g/l, 6.4g/l.It is computed, patient's albumin quotient is 5.8, Immunoglobulin IgA/M/G quotients Respectively 1.7,0.38,4.9.And it is intrathecal without generation reference to extrapolate Immunoglobulin IgA/M/G according to patient's albumin quotient Boundary value is respectively 2.4,1.3,3.8.
Analysis result shows that Immunoglobulin IgG quotient is higher than reference value, and display occurs in slight central nervous system The intrathecal generation of source property, and other immunoglobulin quotients illustrate in reference range without intrathecal generation, are multiple sclerosis One of characteristic index of disease.And according to domestic existing cerebrospinal fluid immunoglobulin reference standard (IgA：0-6mg/L、IgM：0- 13mg/L、IgG：10-40mg/L), three indexs of patient's immunoglobulin are normal, intrathecal generation do not occur, only by brain Spinal fluid inspection result can not be diagnosed as multiple sclerosis.
It is as follows including step the present invention also provides a kind of density calculating method of intrathecal IgA：
Receive concentration value [IgAs of the IgA of input in cerebrospinal fluid1], concentration value [IgAs of the IgA in serum2], Alb exists Concentration value [AlbB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein, QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb), QAlb= [AlbB1]/[AlbB2]；
If QIgA＞ QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
Here, perform this method can be any terminal, for example, it may be computer, computing terminal etc..In terms of certain For calculating terminal, after which is switched on, [IgA is sequentially input1]、[IgA2]、[AlbB1] and [AlbB2], the calculating is whole End can export the generation concentration of patient or the intrathecal IgA of normal person.
In addition, the present invention also provides a kind of intrathecal IgA sensing equipments, which includes：
Receiving module, concentration value [IgAs of the IgA in cerebrospinal fluid measured to real-time reception1], IgA is in serum Concentration value [IgA2], concentration value [AlbBs of the AlB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Computing module, to calculate QLim(IgA)Value and QAlbValue, wherein,
QIgA=[IgA1]/[IgA2], QAlb=[AlbB1]/[AlbB2],
QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2]；
Contrast module, to compare QLim(IgA)Value and QAlbThe size of value；
Output module, to work as QIgA＞ QLim(IgA)When, export the concentration value IgIF A of intrathecal IgACSF=[IgA1]×[1- QLim(IgA)/QIgA]。
Here, the output module is also working as QIgA≤QLim(IgA)When, export the concentration value IgIF A of intrathecal IgACSF=0.
In addition to this, the present invention also provides a kind of computer readable storage medium, the computer readable storage medium is deposited Data processor is contained, when the data processor is executed by processor, realizes following step：
Receive concentration value [IgAs of the IgA of input in cerebrospinal fluid1], concentration value [IgAs of the IgA in serum2], Alb exists Concentration value [AlbB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein, QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb), QAlb= [AlbB1]/[AlbB2]；
If QIgA＞ QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
The foregoing is merely the preferred embodiment of the present invention, are not intended to limit the scope of the invention, every at this Under the inventive concept of invention, the equivalent structure transformation made using present specification or directly/be used in it indirectly His relevant technical field is included in the scope of patent protection of the present invention.

Claims (10)

1. a kind of assay method of intrathecal IgA, which is characterized in that including：
Obtain concentration [IgAs of the IgA in cerebrospinal fluid in sample1], concentration [IgAs of the IgA in serum2], Alb is in cerebrospinal fluid Concentration [AlbB1] and concentration [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein,
QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2],
If QIgA＞ QLim(IgA), then intrathecal IgA generation concentration：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
2. the assay method of intrathecal IgA as described in claim 1, which is characterized in that
QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
3. the assay method of intrathecal IgA as claimed in claim 1 or 2, which is characterized in that
If QIgA≤QLim(IgA), then intrathecal IgA generation concentration：
IgIF ACSF=0.
4. a kind of density calculating method of intrathecal IgA, which is characterized in that including:
Receive concentration value [IgAs of the IgA of input in cerebrospinal fluid1], concentration value [IgAs of the IgA in serum2], Alb is in brain ridge Concentration value [AlbB in liquid1] and concentration value [AlbBs of the Alb in serum2]；
Compare QIgAWith QLim(IgA)Size, wherein,
QIgA=[IgA1]/[IgA2], QLim(IgA)=f (QAlb),
QAlb=[AlbB1]/[AlbB2]；
If QIgA＞ QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=[IgA1]×[1-QLim(IgA)/QIgA]。
5. the density calculating method of intrathecal IgA as claimed in claim 4, which is characterized in that
QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
6. the density calculating method of intrathecal IgA as described in claim 4 or 5, which is characterized in that
If QIgA≤QLim(IgA), then the generation concentration of intrathecal IgA is exported：
IgIF ACSF=0.
7. a kind of intrathecal IgA sensing equipments, which is characterized in that including：
Receiving module, concentration value [IgAs of the IgA in cerebrospinal fluid measured to real-time reception1], concentration values of the IgA in serum [IgA2], concentration value [AlbBs of the AlB in cerebrospinal fluid1] and concentration value [AlbBs of the Alb in serum2]；
Computing module, to calculate QLim(IgA)Value and QAlbValue, wherein,
QIgA=[IgA1]/[IgA2], QAlb=[AlbB1]/[AlbB2],
QLim(IgA)=f (QAlb), QAlb=[AlbB1]/[AlbB2]；
Contrast module, to compare QLim(IgA)Value and QAlbThe size of value；
Output module, to work as QIgA＞ QLim(IgA)When, export the concentration value IgIF A of intrathecal IgACSF=[IgA1]×[1- QLim(IgA)/QIgA]。
8. intrathecal IgA sensing equipments as claimed in claim 7, which is characterized in that
QLim(IgA)=a [(QAlb)2+b2)]1/2- c, a ∈ [0.75,0.8], b ∈ [4.5 × 10-3, 4.9 × 10-3], c ∈ [3.0 × 10-3, 3.2 × 10-3]。
9. intrathecal IgA sensing equipments as claimed in claim 7 or 8, which is characterized in that
The output module is also working as QIgA≤QLim(IgA), export the concentration value of intrathecal IgA：IgIF ACSF=0.
10. a kind of computer readable storage medium, which is characterized in that the computer-readable recording medium storage has data processing Program when the data processor is executed by processor, realizes that such as intrathecal IgA's of claim 4 to 6 any one of them is dense Spend computational methods.
CN201810006469.4A 2018-01-03 2018-01-03 The computational methods and equipment of intrathecal IgA, assay method and computer readable storage medium Pending CN108254573A (en)

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