CN108254570A - 甲胎蛋白定量检测试剂盒 - Google Patents
甲胎蛋白定量检测试剂盒 Download PDFInfo
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Abstract
本发明公开了甲胎蛋白定量检测试剂盒,其特征在于,所述试剂盒包括:甲胎蛋白抗体包被板、甲胎蛋白抗体酶结合物、甲胎蛋白校准品、甲胎蛋白质控品、20倍浓缩洗液。本发明的优点是:检测速度快,能快速检测处是否有甲胎蛋白,检测准确,灵敏度高,易操作。
Description
技术领域
本发明涉及免疫分析医学领域,具体的涉及甲胎蛋白定量检测试剂盒。
背景技术
甲胎蛋白(AFP)是一种糖蛋白,属于白蛋白家族,主要由胎儿肝细胞 及卵黄囊合成。甲胎蛋白在胎儿血液循环中具有较高的浓度,出生后则下降, 至生后2~3月甲胎蛋白基本被白蛋白替代,血液中较难检出,故在成人血 清中含量极低。甲胎蛋白具有很多重要的生理功能,包括运输功能、作为生 长调节因子的双向调节功能、免疫抑制、T淋巴细胞诱导凋亡等。甲胎蛋白 与肝癌及多种肿瘤的发生发展密切相关,在多种肿瘤中均可表现出较高浓 度,可作为多种肿瘤的阳性检测指标。目前临床上主要作为原发性肝癌的血 清标志物,用于原发性肝癌的诊断及疗效监测。
试剂盒用于盛放检测化学成分、药物残留、病毒种类等化学试剂的盒 子。一般医院、制药企业使用。
目前甲胎蛋白的试剂盒检测准确率较差。
发明内容
本发明的目的在于克服现有技术的不足,提供一种快速检测甲胎蛋白的 试剂盒。
本发明通过以下方案实现:
甲胎蛋白定量检测试剂盒,所述试剂盒包括:甲胎蛋白抗体包被板、 甲胎蛋白抗体酶结合物、甲胎蛋白校准品、甲胎蛋白质控品、20倍浓缩洗 液。
优选的,所述的甲胎蛋白抗体包被板为包被有甲胎蛋白抗体的96孔或 48孔的白色微孔板,白色微孔板的孔间平均变异不高于7%。
优选的,所述的抗体酶结合物所用的酶为辣根过氧化物酶,纯度要求 RZ≥2.5,活性≥260U/mL。
优选的,甲胎蛋白质控品包括低值质控品和高值质控品,其中低值质 控品的浓度范围9ng/mL,高值质控品的浓度范围是130ng/mL。
优选的,所述的20倍浓缩洗液包括75.5g/LTris,120g/LNaCl, 5mL/LTween-20,1g/LProclin300。
本发明的优点是:检测速度快,能快速检测处是否有甲胎蛋白,检测准 确,灵敏度高,易操作。
具体实施方式
为了使本发明上述特征和优点更加清楚和容易理解,对本发明的实施方 式作进一步详细描述。
实施例1
甲胎蛋白定量检测试剂盒,所述试剂盒包括:甲胎蛋白抗体包被板、 甲胎蛋白抗体酶结合物、甲胎蛋白校准品、甲胎蛋白质控品、20倍浓缩洗 液。
优选的,所述的甲胎蛋白抗体包被板为包被有甲胎蛋白抗体的96孔或 48孔的白色微孔板,白色微孔板的孔间平均变异不高于7%。
优选的,所述的抗体酶结合物所用的酶为辣根过氧化物酶,纯度要求 RZ≥2.5,活性≥260U/mL。
优选的,甲胎蛋白质控品包括低值质控品和高值质控品,其中低值质 控品的浓度范围9ng/mL,高值质控品的浓度范围是130ng/mL。
优选的,所述的20倍浓缩洗液包括75.5g/LTris,120g/LNaCl, 5mL/LTween-20,1g/LProclin300。
以上所述仅为本发明创造的较佳实施例而已,并不用以限制本发明创 造,凡在本发明创造的精神和原则之内,所作的任何修改、等同替换、改进 等,均应包含在本发明创造的保护范围之内。
Claims (5)
1.甲胎蛋白定量检测试剂盒,其特征在于,所述试剂盒包括:甲胎蛋白抗体包被板、甲胎蛋白抗体酶结合物、甲胎蛋白校准品、甲胎蛋白质控品、20倍浓缩洗液。
2.根据权利要求1所述的甲胎蛋白定量检测试剂盒,其特征在于,所述的甲胎蛋白抗体包被板为包被有甲胎蛋白抗体的96孔或48孔的白色微孔板,白色微孔板的孔间平均变异不高于7%。
3.根据权利要求1所述的甲胎蛋白定量检测试剂盒,其特征在于,所述的抗体酶结合物所用的酶为辣根过氧化物酶,纯度要求RZ≥2.5,活性≥260U/mL。
4.根据权利要求1所述的甲胎蛋白定量检测试剂盒,其特征在于,甲胎蛋白质控品包括低值质控品和高值质控品,其中低值质控品的浓度范9ng/mL,高值质控品的浓度范围是130ng/mL。
5.根据权利要求1所述的甲胎蛋白定量检测试剂盒,其特征在于,所述的20倍浓缩洗液包括75.5g/LTris,120g/LNaCl,5mL/LTween-20,1g/LProclin300。
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CN102095868A (zh) * | 2009-12-11 | 2011-06-15 | 上海裕隆生物科技有限公司 | 甲胎蛋白化学发光定量检测试剂盒 |
CN102866254A (zh) * | 2012-09-21 | 2013-01-09 | 潍坊市康华生物技术有限公司 | 一种高灵敏度孕中期唐氏综合症产前筛查试剂盒及其制备和检测方法 |
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CN102095868A (zh) * | 2009-12-11 | 2011-06-15 | 上海裕隆生物科技有限公司 | 甲胎蛋白化学发光定量检测试剂盒 |
CN102866254A (zh) * | 2012-09-21 | 2013-01-09 | 潍坊市康华生物技术有限公司 | 一种高灵敏度孕中期唐氏综合症产前筛查试剂盒及其制备和检测方法 |
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