CN108254348A - A kind of the pH responsive types fluorescent optical sensor and construction method of high-throughput detection active microorganism - Google Patents
A kind of the pH responsive types fluorescent optical sensor and construction method of high-throughput detection active microorganism Download PDFInfo
- Publication number
- CN108254348A CN108254348A CN201810097374.8A CN201810097374A CN108254348A CN 108254348 A CN108254348 A CN 108254348A CN 201810097374 A CN201810097374 A CN 201810097374A CN 108254348 A CN108254348 A CN 108254348A
- Authority
- CN
- China
- Prior art keywords
- gel
- fluorescence
- transducer
- prepared
- micella
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Abstract
A kind of the pH responsive types fluorescent optical sensor and its construction method of high-throughput detection active microorganism, include the following steps：1）Prepared by fluorescent nano material, dissolve phosphatide polyethylene glycol using ethyl alcohol and 5 (N hexadecanoyls) amino fluoresceins are yellow, be sufficiently mixed, be slowly dropped into pure water and prepare fluorescence nano micella；2）Prepared by transducer gel, fluorescence nano micella is added in 1 ~ 3% hot agar solution and is sufficiently mixed, condensation prepares fluorescence sense gel；3）Sensor is built, and container is divided into two parts using water phase filter membrane, lower part is packed into transducer gel, and top is packed into liquid microbe culture medium.It is an advantage of the invention that culture unit and detection unit are separated, by the variation for detecting fluorescence signal in transducer gel, it realizes that the sour aerogen of active production is quick, detect in real time, with respect to classic flat-plate method, the sensor is theoretical based on traditional culture, easy to operate, detection time is short, can realize the high flux screening of microorganism in different materials.
Description
Technical field
The invention belongs to microorganism detection fields, are related to the detection of trace active microorganism, specifically a kind of high-throughput inspection
Survey the pH responsive types fluorescent optical sensor and construction method of active microorganism.
Background technology
In recent years, food safety affair both domestic and external emerges in an endless stream, and not only affects market order, returns the public and brings
Certain fear, such as the microbial contamination event of the natural milk powder of perseverance occurred in the recent period, although final certification is not clostridium botulinum,
But also show the worry that society pollutes microorganism in food indirectly.
The colony counting method of microorganism detection is conventionally used to, accuracy, sensitivity are higher, but the experiment being related to is more, behaviour
Make it is loaded down with trivial details, the long period is needed to prepare and ending, and to there are a large amount of personnel to participate in.So there is an urgent need to accurate, time saving, provinces
Power and the method for quickly detecting of cost-saving, at present mainly method have the microorganism testing scraps of paper, biochemistry detection method,
ATP methods, flow cytometer etc..
The Perrifilm of 3M companiesTM Plate series microorganism testing slices can detect total plate count, coliform respectively
Counting, mould and yeast counts etc., paper disk method are all based on tradition culture theory with National Standard Method, in microorganism recall rate unanimously
Property it is preferable, and bacterium colony is typical, easily judges, but detects that flux is not high, and testing the sensitivity of the scraps of paper still has the need further improved
It will.
Round pcr synthesizes DNA fragment specific using external enzymatic reaction, then identifies bacterium by amplified production.By
In PCR high sensitivities, the copy gene of a bacterium can be theoretically detected, therefore only the short time is needed to increase in the detection of bacterium
Bacterium does not increase bacterium even, you can is screened, saved the plenty of time by PCR, but round pcr is to need higher personnel requirement
And laboratory environment, and viable bacteria and dead bacterium can not be accomplished to distinguish, while the extraction of target gene also has one in complex matrices
Fixed difficulty.
ATP biloluminescence methods utilize ATP bioluminescent assays technology and body cell clearance technique, measure bacterial ATP and body
Cell ATP, the amount of bacterial ATP is directly proportional to bacterial population, and so as to fulfill the detection of microorganism, but this method can not be distinguished
Go out the type of microorganism, be only used for the monitoring of microorganism total amount.
After flow cytometry utilizes Pretreated, single cell suspension is prepared, is analyzed after specific stain, is analyzed
Speed is fast, can simultaneously be analyzed with multi-parameter, but expensive equipment, and the concentration of analysis needs the higher order of magnitude, micro- life to trace
Object analytical error is larger.
In conclusion although at present there are many method and technology of microorganism detection, major part all exists certain
The problem of, and it is larger with traditional culture theory difference, as a result there are risks in consistency, and the detection of trace microorganism is still
There are certain challenges.Therefore, develop a kind of high sensitivity, it is easy to operate and can realize batch samples are carried out it is quick
The method of detection becomes the problem of in the urgent need to address.
Invention content
It is an object of the invention to overcome the shortcomings of existing trace microorganism detection technology, development is a kind of based on glimmering in real time
The pH responsive types of the high monitoring analytical technology of light, easy to operate, high sensitivity, detection flux-high throughput detection active microorganism
Fluorescent optical sensor and construction method.
The purpose of the present invention is achieved through the following technical solutions：A kind of pH of high-throughput detection active microorganism is quick
Sense type fluorescent optical sensor, transducer gel, water phase filter membrane and liquid microbe culture medium including being assembled in special container form,
Transducer gel is separated by with liquid microbe culture medium by water phase filter membrane and transducer gel is located at container bottom, the transducer gel
It is to be made of the fluorescence nano micella being fixed in agar gel.
The special container is plastic tube of the PE materials with capping, concrete structure as shown in Fig. 2, be made of 3 parts,
Bottom is sensor, and middle part is microculture part, and top is capping, and bottom can penetrate fluorescence, with the aqueous filter in middle part
Film is isolated.
Construction method includes：
1）It is dissolved in ethyl alcohol and being sufficiently mixed using phosphatide polyethylene glycol and 5- (N- hexadecanoyls) amino fluorescein Huang, gradually instill pure
Fluorescence nano micella is prepared in water；
2）Agar hot solution is prepared, adds in step 1）The fluorescence nano micella of middle preparation is sufficiently mixed, by fluorescence nano after cooling
Micella is fixed in agar gel；
3）Transducer gel and the separation of particular liquid culture medium are prepared suitable for microorganism detection not of the same race using water phase filter membrane
Fluorescent optical sensor.
Construction method is as follows：
1）It is prepared by fluorescence nano micella：Using phosphatide polyethylene glycol and 5- (N- hexadecanoyls) amino fluorescein Huang according to 1：10~1：
50 molar ratios, which are dissolved in chromatographic grade absolute ethyl alcohol, to be sufficiently mixed, and is gradually instilled in pure water and is prepared fluorescence nano micella, preparation
Nano-micelle stores after using 20 mM PBS dialysis purifications at 4 DEG C；
2）The preparation of transducer gel：Using 1 ~ 3% 50-70 DEG C of hot agar solution of agar preparation, according to volume ratio（Hot agar is molten
Liquid/fluorescence nano micella mother liquor）10：1~30：1 addition fluorescence nano micella fixes fluorescence nano micella after cooling, prepare saturating
The high transducer gel of lightness, the grain size of nano-micelle therein in 20 nm-200 nm, excitation and transmitting respectively in 480 nm and
520 nm.The concentration range of the agar gel of preparation is 1%-3%, for fixing fluorescence nano micella, prepares the high biography of transparency
Feel gel.
3）It is prepared by fluid nutrient medium：With reference to LB（Luria-Bertani）The preparation method of culture medium, in 950 ml deionizations
10 g of tryptone, yeast extract 10 g of 5 g, NaCl are added in water, shakes container until solute dissolves；With 5 mol/L
NaOH (about 0.2 ml) adjusts pH value to 7.0；1 L is settled to deionized water；In 15 psi (1.05 kg/cm2) steam under high pressure
20 min of vapour sterilizing；
4）The assembling of sensor：Transducer gel is packed into special container bottom, will be sensed using 0.22 ~ 0.45 μm of water phase filter membrane
Gel and fluid nutrient medium are detached.
In the present invention, the fluorescence nano micella is the package that pH sensitive fluorescence substances are completed in self assembly, wherein phosphatide
Polyethylene glycol as micellar carrier, 5- (N- hexadecanoyls) amino fluorescein Huangs due to hydrophobic aliphatic chain and self assembly is in nanometer
Microsphere surface, 5- (N- hexadecanoyls) amino fluorescein Huangs, can be with the speeds of growth of real time reaction microorganism to pH sensitivities.
Compared with prior art, the beneficial effects of the invention are as follows：
1）Novel pH Sensitive type fluorescence nano micella：Fluorescence nano micella fixes pH responsive type fluorescence by way of self assembly
Dyestuff, fluorescence nano micella size tunable can realize the package of a large amount of fluorescent materials, be combined and can effectively consolidated with agar gel
Determine fluorescent material.
2）It is higher with conventional method consistency：It is theoretical based on tradition culture, it is referred to national standard method and prepares liquid
Culture medium can realize the quick detection of most of microbe, can effectively distinguish viable bacteria and dead bacterium and classic flat-plate counting side
Method consistency is high.
3）Monitoring in real time：Sensor includes fluid nutrient medium and transducer gel, and fluid nutrient medium cultivates microorganism,
Transducer gel can observe the Growth trends of microorganism in real time.
4）High sensitivity：Transducer gel includes highly sensitive fluorescent dye material, and microorganism growth leads to the glimmering of transducer gel
Optical signal changes, and realizes the quick detection of microorganism, and theoretically there are one viable bacteria in the presence of that can be detected, bacterial content is got over
Height, detection time is shorter, and compared to colony counting method, analysis time is greatly shortened.
Description of the drawings
Fig. 1 is the variation of fluorescence micella fluorescence signal in buffer solution in different pH value of pH responsive types；
Fig. 2 is the structure diagram of the fluorescent optical sensor of pH responsive types, in figure：1st, water phase filter membrane；2nd, pH responsive types fluorescence nano
Micella；3rd, transducer gel；4th, fluid nutrient medium；5th, upper cover is sealed；
Fig. 3 is the fluorescence signal response schematic diagram during Escherichia coli Growth, and solid black lines above are high concentration microorganism
Sample, grey filled lines below are low concentration microbiological specimens.
Specific embodiment
With reference to specific embodiment, the present invention is further explained.
It is prepared by 1. pH responsive type fluorescence nanos micella of embodiment
PH responsive types fluorescence nano micella preparation method provided in an embodiment of the present invention includes：Using phosphatide polyethylene glycol and 5-
(N- hexadecanoyls) amino fluorescein Huang is according to 1：10~1：50 molar ratios, which are dissolved in ethyl alcohol, to be sufficiently mixed, and is added dropwise to pure water
In, it is sufficiently stirred, phosphatide polyethylene glycol is made to be self-assembled into nano-micelle, 5- (N- hexadecanoyls) amino fluorescein Huangs are because of hydrophobic chain
Effect fluorescence signal group is fixed on nano-micelle surface.
The ultimate density of the fluorescence nano micella in aqueous solution should can make nanometer in 1 ~ 10 mg/mL, excessively high concentration
The grain size of micella becomes larger, and nano-micelle grain size of the invention is between 20 ~ 200 nm.
The grain size of the nano-micelle can become larger with the increase of mixing time, the present invention used in stirring when
Between at 1 ~ 3 hour.
The fluorescence nano micella reply pH is sensitive, and pH response ranges are between pH 5 ~ 9, the change of fluorescence response
Change as shown in Figure 1.Typical fluorescent dye is yellow for 5- (N- hexadecanoyls) amino fluorescein, and chain alkyl chain is contained in end, has
There is stronger hydrophobic effect, the fluorescence excitation of the nano-micelle micella and transmitting are 480 nm and 520 nm respectively.
The structure of 2. pH responsive type fluorescent optical sensors of embodiment
The structure of pH responsive types fluorescent optical sensor provided in an embodiment of the present invention includes：1. pH responsive type fluorescence nano micellas；2.
Transducer gel；3. fluid nutrient medium；4. water phase filter membrane；5. seal upper cover.First with pH responsive type fluorescence nano micellas and fine jade
Fat prepares transducer gel, is placed in the bottom of special container shown in Fig. 2, using 0.22 ~ 0.45 μm of water phase filter membrane by transducer gel
It is separated with fluid nutrient medium, detaches the structure for completing pH responsive type fluorescent optical sensors with the external world using sealing upper cover.
The pH responsive types fluorescence nano micella is prepared according to the step of embodiment 1, and fluorescence nano micella can wrap up simultaneously
Fixed multiple luminescent dye molecules provide high intensity fluorescence signal, while fixed luminescent dye molecule, make it not to Liquid Culture
Base is spread.
The transducer gel is prepared using 1 ~ 3% agar hot solution mixing pH responsive type fluorescence nanos micella, molten in agar
Liquid temperature is mixed into stirring cooling when being less than 70 DEG C, transducer gel is in the high grade of transparency, can be read out with mating fluorescence spectrum instrument
Fluorescence signal.
The water phase filter sizes size can be effectively isolated common microbiological, avoid microorganism at 0.22 ~ 0.45 μm
It is spread into transducer gel.
The fluid nutrient medium cultivates theoretical preparation according to tradition, has certain selectivity for different microorganisms.
The real-time detection of 3. Escherichia coli of embodiment
The embodiment of the present invention provides the method using pH responsive types fluorescent optical sensor detection Escherichia coli, and detecting step includes：1、
Sample pre-treatments；2nd, in the sample merging fluid nutrient medium handled well；3rd, sensor is put into dedicated fluorescence detector；4、
Fluorescence signal value is read, obtains the real-time growth curve of microorganism, the results are shown in Figure 3 for growth curve,Solid black lines are high concentration
Microbiological specimens, grey filled lines are low concentration microbiological specimens, the results showed that content of microorganisms is higher, and fluorescence signal growth is got over
Soon.
The preceding sample pre-treatments need the form for sample（Solid, liquid etc.）Handled, need when necessary into
Row is simple to increase bacterium processing.
The fluid nutrient medium needs the microbe species for detection, is designed according to culture theory with certain choosing
The culture medium of selecting property avoids the growth of miscellaneous bacteria.
The fluorescence detector has the excitation wavelength of 480 nm, can detect the transmitted wave at 520 nm, can see in real time
The fluorescence signal variation in transducer gel is examined, and draws curve.
Claims (7)
1. a kind of pH responsive type fluorescent optical sensors of high-throughput detection active microorganism, it is characterised in that：It is special including being assembled in
Transducer gel, water phase filter membrane and liquid microbe culture medium composition, transducer gel in container lead to liquid microbe culture medium
Cross that water phase filter membrane is separated by and transducer gel is located at container bottom, the transducer gel is received by the fluorescence being fixed in agar gel
Rice glue beam is formed.
2. sensor according to claim 1, it is characterised in that：Modeling with capping of the special container for PE materials
Expects pipe.
3. a kind of structure side of the pH responsive type fluorescent optical sensors of high-throughput detection active microorganism as described in claim 1
Method, it is characterised in that：Construction method includes the following steps：
1）It is dissolved in ethyl alcohol and being sufficiently mixed using phosphatide polyethylene glycol and 5- (N- hexadecanoyls) amino fluorescein Huang, gradually instill pure
Fluorescence nano micella is prepared in water；
2）Agar hot solution is prepared, adds in step 1）The fluorescence nano micella of middle preparation is sufficiently mixed, by fluorescence nano after cooling
Micella is fixed in agar gel；
3）Transducer gel and the separation of particular liquid culture medium are prepared suitable for microorganism detection not of the same race using water phase filter membrane
Fluorescent optical sensor.
4. construction method according to claim 3, it is characterised in that：It is as follows：
1）It is prepared by fluorescence nano micella：Using phosphatide polyethylene glycol and 5- (N- hexadecanoyls) amino fluorescein Huang according to 1：10~1：
50 molar ratios, which are dissolved in chromatographic grade absolute ethyl alcohol, to be sufficiently mixed, and is gradually instilled in pure water and is prepared fluorescence nano micella, preparation
Nano-micelle stores after using 20 mM PBS dialysis purifications at 4 DEG C；
2）The preparation of transducer gel：Using 1 ~ 3% 50-70 DEG C of hot agar solution of agar preparation, according to volume ratio 10：1~30：1
Addition fluorescence nano micella, fix fluorescence nano micella after cooling, prepare the high transducer gel of transparency；
3）It is prepared by fluid nutrient medium：With reference to LB（Luria-Bertani）The preparation method of culture medium, in 950 ml deionized waters
10 g of tryptone, yeast extract 10 g of 5 g, Nacl are added in, shakes container until solute dissolves；With NaOH tune pH value extremely
7.0；1 L is settled to deionized water；High pressure steam sterilization；
4）The assembling of sensor：Transducer gel is packed into special container bottom, will be sensed using 0.22 ~ 0.45 μm of water phase filter membrane
Gel and fluid nutrient medium are detached.
5. construction method according to claim 3 or 4, it is characterised in that：Transducer gel wherein prepared by step 2 is transparent
Degree is high, and the grain size of nano-micelle therein excitation and emits respectively in 480 nm and 520 nm in 20 nm-200 nm.
6. construction method according to claim 3 or 4, it is characterised in that：The fluorescence nano micella is that self assembly is completed
The package of pH sensitive fluorescence substances, wherein phosphatide polyethylene glycol as micellar carrier, 5- (N- hexadecanoyls) amino fluorescein Huangs by
In with hydrophobic aliphatic chain and self assembly on nanoparticle surface, 5- (N- hexadecanoyls) amino fluorescein Huangs, can be with to pH sensitivities
The speed of growth of real time reaction microorganism.
7. construction method according to claim 3 or 4, it is characterised in that：The concentration range of the agar gel of preparation is 1%-
3%, for fixing fluorescence nano micella, prepare the high transducer gel of transparency.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097374.8A CN108254348B (en) | 2018-01-31 | 2018-01-31 | pH sensitive fluorescent sensor for high-throughput detection of active microorganisms and construction method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097374.8A CN108254348B (en) | 2018-01-31 | 2018-01-31 | pH sensitive fluorescent sensor for high-throughput detection of active microorganisms and construction method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108254348A true CN108254348A (en) | 2018-07-06 |
| CN108254348B CN108254348B (en) | 2020-11-17 |
Family
ID=62742753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810097374.8A Active CN108254348B (en) | 2018-01-31 | 2018-01-31 | pH sensitive fluorescent sensor for high-throughput detection of active microorganisms and construction method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108254348B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109030439A (en) * | 2018-07-09 | 2018-12-18 | 广州华澳生物科技有限公司 | A kind of preparation method and purposes of magnetism rare-earth fluorescent microballoon |
| CN110317605A (en) * | 2018-03-30 | 2019-10-11 | 中国科学院过程工程研究所 | A kind of preparation method and purposes of the fluorescence probe based on self-assembled micelle |
| WO2020148571A1 (en) | 2019-01-16 | 2020-07-23 | Ivan Tomka | Method and device for acid- or base concentration measurement |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6306598B1 (en) * | 1992-11-13 | 2001-10-23 | Regents Of The University Of California | Nucleic acid-coupled colorimetric analyte detectors |
| US6692775B2 (en) * | 2000-03-13 | 2004-02-17 | Aqua Med, Inc. | Bactericidal gel and method for treating burns and dermal lesions |
| CN102994387A (en) * | 2012-09-17 | 2013-03-27 | 天津工业生物技术研究所 | High throughput screening method of aminopeptidase and high-yield strain thereof |
| CN103937658A (en) * | 2014-03-28 | 2014-07-23 | 武汉介观生物科技有限责任公司 | Rare cell detection chip and application thereof |
| CN105194663A (en) * | 2015-10-19 | 2015-12-30 | 中国科学院生物物理研究所 | Micelle polypeptide vaccine with poly(ethylene glycol)-phosphatidylethanolamine as carrier |
| CN106172380A (en) * | 2016-07-14 | 2016-12-07 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of the blade face controlled carrier micelle of affinity |
| CN106420611A (en) * | 2016-11-11 | 2017-02-22 | 天津城建大学 | PH-sensitive chitosan drug-carrying micelle with targeting and fluorescent characteristics and preparation method thereof |
| CN106556599A (en) * | 2016-10-25 | 2017-04-05 | 上海美凯纯生物科技有限公司 | A kind of method for rapid inspecting animalcule |
-
2018
- 2018-01-31 CN CN201810097374.8A patent/CN108254348B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6306598B1 (en) * | 1992-11-13 | 2001-10-23 | Regents Of The University Of California | Nucleic acid-coupled colorimetric analyte detectors |
| US6692775B2 (en) * | 2000-03-13 | 2004-02-17 | Aqua Med, Inc. | Bactericidal gel and method for treating burns and dermal lesions |
| CN102994387A (en) * | 2012-09-17 | 2013-03-27 | 天津工业生物技术研究所 | High throughput screening method of aminopeptidase and high-yield strain thereof |
| CN103937658A (en) * | 2014-03-28 | 2014-07-23 | 武汉介观生物科技有限责任公司 | Rare cell detection chip and application thereof |
| CN105194663A (en) * | 2015-10-19 | 2015-12-30 | 中国科学院生物物理研究所 | Micelle polypeptide vaccine with poly(ethylene glycol)-phosphatidylethanolamine as carrier |
| CN106172380A (en) * | 2016-07-14 | 2016-12-07 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of the blade face controlled carrier micelle of affinity |
| CN106556599A (en) * | 2016-10-25 | 2017-04-05 | 上海美凯纯生物科技有限公司 | A kind of method for rapid inspecting animalcule |
| CN106420611A (en) * | 2016-11-11 | 2017-02-22 | 天津城建大学 | PH-sensitive chitosan drug-carrying micelle with targeting and fluorescent characteristics and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| FOUZI MOUFFOUK: "Development of a highly sensitive bacteria detection assay using fluorescent pH-responsive polymeric micelles", 《BIOSENSORS AND BIOELECTRONICS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317605A (en) * | 2018-03-30 | 2019-10-11 | 中国科学院过程工程研究所 | A kind of preparation method and purposes of the fluorescence probe based on self-assembled micelle |
| CN109030439A (en) * | 2018-07-09 | 2018-12-18 | 广州华澳生物科技有限公司 | A kind of preparation method and purposes of magnetism rare-earth fluorescent microballoon |
| WO2020148571A1 (en) | 2019-01-16 | 2020-07-23 | Ivan Tomka | Method and device for acid- or base concentration measurement |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108254348B (en) | 2020-11-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xue et al. | Microfluidic colorimetric biosensors based on MnO2 nanozymes and convergence–divergence spiral micromixers for rapid and sensitive detection of salmonella | |
| Mauerhofer et al. | Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology | |
| Xue et al. | An impedance biosensor based on magnetic nanobead net and MnO2 nanoflowers for rapid and sensitive detection of foodborne bacteria | |
| CN108254348A (en) | A kind of the pH responsive types fluorescent optical sensor and construction method of high-throughput detection active microorganism | |
| CN1957089B (en) | Measuring contamination | |
| CN104007104B (en) | Quickly detect kit and the using method thereof of organophosphorus pesticide | |
| CN105122058A (en) | Method to identify bacterial species by means of gas chromatography/mass spectrometry in biological samples | |
| Xing et al. | Microfluidic biosensor for one-step detection of multiplex foodborne bacteria ssDNA simultaneously by smartphone | |
| US9511365B2 (en) | Sensor housing and reagent chemistry | |
| CN102520168B (en) | Immunosensor for detecting aspergillus parasiticus used for producing aflatoxin and preparing method thereof | |
| Junker et al. | On-line and in-situ monitoring technology for cell density measurement in microbial and animal cell cultures | |
| Alam et al. | Status and advances in technologies for phosphorus species detection and characterization in natural environment-A comprehensive review | |
| EP1529213B1 (en) | The method and apparatus for determining the number of living cells in a test fluid | |
| JPH02503747A (en) | Qualitative and/or quantitative testing method for microorganisms and equipment for carrying out the method | |
| CN110241064A (en) | A kind of building and its application of the nucleic acid-protein compound allosteric type microbial whole-cell sensor for mercury ion detecting | |
| Zhang et al. | Acute heavy metal toxicity test based on bacteria-hydrogel | |
| US20240035974A1 (en) | Method and apparatus for rapid detection of bacterial contamination | |
| Singh et al. | Evaluation of biomass | |
| Berlina et al. | Development of lateral flow test-system for the immunoassay of dibutyl phthalate in natural waters | |
| Urriza-Arsuaga et al. | Luminescence-based sensors for bioprocess applications | |
| Verma et al. | Fiber optic biosensor for the detection of Cd in milk | |
| CN101477105A (en) | Rapid measuring method for high-salt trade waste BOD | |
| CN111504959A (en) | C-peptide rare earth fluorescent microsphere kit, detection card and preparation method thereof | |
| CN204101457U (en) | A kind of reaction unit detecting microorganism for ATP bioluminescence method | |
| CN110283769A (en) | A kind of building and its application of the nucleic acid-protein compound allosteric type microbial whole-cell sensor for lead ion detection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |