CN108254233A - A kind of nicotine solution standard substance and its preparation method and application - Google Patents
A kind of nicotine solution standard substance and its preparation method and application Download PDFInfo
- Publication number
- CN108254233A CN108254233A CN201711483723.1A CN201711483723A CN108254233A CN 108254233 A CN108254233 A CN 108254233A CN 201711483723 A CN201711483723 A CN 201711483723A CN 108254233 A CN108254233 A CN 108254233A
- Authority
- CN
- China
- Prior art keywords
- nicotine
- standard substance
- substance
- standard
- nicotine solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N2001/2893—Preparing calibration standards
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The present invention relates to a kind of nicotine solution standard substances and its preparation method and application.The nicotine solution standard substance is made of nicotine purity rubric substance and solvent, solvent be isopropanol or water, a concentration of 5.00 9.80mg/mL of nicotine solution.Nicotine solution standard substance provided by the invention by the selection of solvent and concentration, through uniformity and stability test, Evaluation of Uncertainty, can be used as standard substance.The concentration for the nicotine standard solution that nicotine detection and analysis is used is generally no greater than 2.0mg/mL, which not only conforms with Security Administration of the country to hazardous chemical, and the analysis that can meet different content nicotine sample measures.
Description
Technical field
The invention belongs to tobacco chemistry inspection technology fields, and in particular to a kind of nicotine solution standard substance and its preparation side
Method and application.
Background technology
Nicotine is the addiction feature ingredient of tobacco and tobacco product, belongs to the typical receptor excitement of nicotinic acetylcholine receptor
Agent.The height of nicotine content is an important indicator for influencing tobacco leaf and cigarette quality, greatly influences the strength of cigarette smoking.
Nicotine is the important chemical composition for needing to detect in tobacco and tobacco product, cigarette smoke and electronic cigarette liquid, in nicotine content
In detection, nicotine standard substance is unavoidably used.
2015, according to《Safety management of dangerous chemical products regulations》(State Council Decree the 591st) provides, national security production
The publication of 10 ministries and commissions such as supervision and management general bureau《Hazardous chemical catalogue》(2015 editions) by nicotine (No. 1097, CAS 54-11-
5) and sulphation nicotine (No. 1041, CAS 65-30-5) is classified as toxic articles.Therefore, the acquisition of nicotine standard substance is by tight
Lattice control, this also directly affects the detection and analysis of daily nicotine.
Using the safety of nicotine and safety management risk aspect, rational nicotine solution form are reduced from raising
Security Administration can be met and meet the requirement of daily nicotine detection and analysis.At present, there is no such nicotine is molten
Liquid standard substance sees report.
Standard substance is a kind of material or substance, one or more characteristic value stabilization uniform enough and by it is good really
It is fixed, for calibration measurement system, evaluation process of measurement or it is material assignment.Under normal circumstances, standard substance, which refers to, card reference substance
Matter, one or more characteristic value are determined with the program for establishing traceability, are allowed to trace to the source to the expression accurately reappeared the spy
The units of measurement of property value, each determining characteristic value have the uncertainty of given confidence level.On August 1st, 20017, China
Formally issue and implement《Standard substance/standard sample producer's capability Approval criterion》In, to the group of the standard substance producer
Knit production management, the assignment of standard substance and statistical method, uncertainty evaluation, the performance indicator that reaches needed for standard substance etc.
Propose higher requirement.
Invention content
The purpose of the present invention is to provide a kind of nicotine solution standard substances, lack and can meet so as to solve the prior art
Security Administration, and the problem of the nicotine solution standard substance of daily nicotine detection and analysis requirement can be met.
Second object of the present invention is the preparation method of above-mentioned nicotine solution standard substance.
Third object of the present invention is to provide the application of above-mentioned nicotine solution standard substance.
To achieve the above object, the technical solution adopted in the present invention is：
A kind of nicotine solution standard substance, is made of nicotine purity rubric substance and solvent, solvent be isopropanol or water, cigarette
A concentration of 5.00-9.80mg/mL of aqueous slkali.
Nicotine solution standard substance provided by the invention by the selection of solvent and concentration, is examined through uniformity and stability
It tests, Evaluation of Uncertainty, can be used as standard substance.The concentration one for the nicotine standard solution that nicotine detection and analysis is used
As no more than 2.0mg/mL, which not only conforms with Security Administration of the country to hazardous chemical, and
And the analysis that can meet different content nicotine sample measures.
The nicotine purity rubric substance is GBW (E) 100264 in the number of State Standard Matter Research Centre.
Technical solution is used by the preparation method of the nicotine solution standard substance of the present invention：
A kind of preparation method of nicotine solution standard substance, includes the following steps：Nicotine purity rubric substance is dissolved in
In solvent, compound concentration is the nicotine solution of 5.00-9.80mg/mL.
Nicotine purity rubric substance is weighed in beaker, adding in solvent dissolves it and solution is transferred in volumetric flask,
Beaker is rinsed repeatedly with solvent, washing lotion is incorporated in volumetric flask, and solvent is added in into volumetric flask makes a concentration of 5.00-
9.80mg/mL。
Prepared nicotine solution is filling in Brown Glass Brown glass bottles and jars only, it seals up to nicotine solution standard substance product.Institute
It states nicotine solution standard substance product and outer packing is carried out using black kraft paper bag.The nicotine solution standard substance product storage
In -18 DEG C~-16 DEG C of environment.
The nicotine solution standard substance prepared using the above method, uniformity and stability is good, magnitude tracing of definite value can
It leans on, synthesize uncertain small, improve the reliability and comparativity of nicotine detection work.
The nicotine solution standard substance of the present invention has in nicotine or using in terms of nicotine in the test of compound as standard items
Good application effect.
The measure of nicotine in the measure of nicotine, electronic cigarette liquid, heating are not burnt cigarette in applied to cigarette mainstream flue gas
The use of the nicotine solution standard substance that solvent is isopropanol is standard items during the measure of middle nicotine.
In the measure of nicotine, tobacco and tobacco product in applied to tobacco and tobacco product during the measure of vegetable soda, make
It is standard items with the nicotine solution standard substance that solvent is water.
The standard substance that nicotine solution standard substance using the present invention is GBW (E) 100264 with number prepares standard work
Make curve to test same sample, obtained result is consistent, it was demonstrated that the nicotine solution product is used as standard substance
Feasibility.
Specific embodiment
Embodiments of the present invention are described further with reference to specific embodiment.
Embodiment 1
The nicotine solution standard substance of the present embodiment, is made of nicotine purity rubric substance and isopropanol, nicotine solution
A concentration of 9.80mg/mL.
The preparation method of the nicotine solution standard substance of the present embodiment, includes the following steps：
1) it weighs：The substance that the number for weighing 19.6000g State Standard Matter Research Centre is GBW (E) 100264 in
In 50mL beakers, adding in isopropanol makes its dissolving, and be transferred in 2000mL volumetric flasks；
2) it is quantitative：Small beaker is rinsed repeatedly with isopropanol, washing lotion is incorporated in volumetric flask, and adds in isopropanol mixing,
Scale is settled to, it is 9.80mg/mL to determine its actual concentrations；
3) it is the nicotine solution of a concentration of 9.80mg/mL is filling in the Brown Glass Brown glass bottles and jars only of 50mL, sealing.
Nicotine solution product carries out outer packing using black kraft paper bag, is stored in -18 DEG C of refrigerator-freezer.
The preparation method of embodiment 1 is repeated 10 times, and 400 bottles of nicotine solution products are obtained.The nicotine solution of the present embodiment
It is as follows as standard substance evaluation rubric：
(1) uniformity and stability test
Uniformity testing：According to《The generic principles and Principle of Statistics of standard substance definite value》In (JJF 1343-2012)
(as 200 ＜ N≤500,15) extracting unit number is no less than, from the isopropyl dispensed for requirement to uniformity testing sample size
In alcohol 15 bottles are successively extracted according to encapsulation in nicotine solution standard substance sample.Three samples of every bottle of preparation, are made 45 examinations altogether
Sample, and each sample is numbered.According to《The measure gas chromatography of nicotine in Cigarette grain phase》(GB/T 23355-
2009) method specified in carries out nicotine content test, and uniformity testing test result is as shown in table 1.According to ISO guide
35-2006, it is for statistical analysis using F methods of inspection, less than F0.05(14,30) show between sample in 95% water of fiducial probability
There is no significant differences under flat, and the nicotine solution standard substance sample after packing is uniform.
1 uniformity testing result of table
Short-term stability is examined：It is provided according to ISO guide 35-2006, the development of standard items is needed to study transport and be turned
Requirement of the journey to environment is moved past, high temperature experiment (60 DEG C, 14 days), high humidity experiment (90% need to be carried out to standard items sample after packing
± 5%, 14 day), illumination experiment (illumination 4500lx ± 500lx, 14 days), and respectively at 0 day, 7 days, 14 days to by high temperature,
Standard items sample after high humidity, illumination is detected, to investigate standard items sample variation situation.
It is deposited in -18 DEG C of refrigerator-freezers in view of " nicotine solution standard substance in isopropanol ", environment is high humidity (relative humidity
More than 95%) it is protected from light, and " nicotine solution standard substance in isopropanol " is protected from light in transit using black kraft paper bag outer packing
Transport, therefore when " nicotine solution standard substance in isopropanol " short-term stability is investigated, high temperature is only limitted to nicotine characteristic value
Influence." nicotine solution standard substance in isopropanol " sample is investigated under the conditions of 60 DEG C, the 0th day, the 7th day, the 14th day it is each
Constituent content situation of change.
9 bottles of " nicotine solution standard substance in isopropanol " samples are taken out from refrigerator-freezer, wherein 6 bottles are positioned over set temperature and are
In 60 DEG C of baking ovens, remaining 3 bottles are directly tested, and 3 bottles of tests are taken out within the 7th day from baking oven, takes out within the 14th day and surveys by last 3 bottles
Examination, the equal replication of every bottle of sample 3 times.According to《The generic principles and Principle of Statistics of standard substance definite value》(JJF 1343-
2012) stability of average value consistency-checking method evaluation criterion product, formula are expressed as specified in：
T distribution bilateral Tables are looked into understand：t4 0.05=2.776, it measures and statistical result is as shown in table 2.Short-term stability
Property the experimental results showed that, the t values that the short-term stability of " nicotine solution standard substance in isopropanol " is examined are respectively less than critical value, by
This illustrates that the nicotine solution standard substance packaging of embodiment 1 can ensure to meet generally to environment in transport and transfer process
It asks.
Nicotine solution standard substance high-temperature stability inspection data in 2 isopropanol of table
Long-time stability are examined：According to《Primary standard substance》(JJG 1006-1994) is provided, national grade ii standard product are long
Stability requirement June phase and more than.In view of " nicotine solution standard substance in isopropanol " sample under cryogenic freezing state
Stability tests the standard items sample after packaging being placed at -18 DEG C preserving for a long time, and respectively 0 month, 2 months, April, August,
14 months content detections that nicotine is carried out to sampling.Each time point randomly selects 3 bottles of samples, each sample replication 3 times.Often
During secondary measurement, standard working curve is remake.Long-time stability experimental result is shown in Table 3.
Nicotine solution standard substance long-time stability data in 3 isopropanol of table
Provided according to ISO guide 35-2006, due in not no accurate model reflection solution nicotine content it is true
Changing rule, therefore test and use straight line empirically model.
Linear equation：Y=b+ax.
The standard deviation of residual error：
With the relevant uncertainty of slope：
When | a |<t3 0.05×s(a)When, slope is inapparent, is had good stability.
Under 95% fiducial probability, degree of freedom 3, the quantile value of two-tailed test t distributions is 3.2.
It is provided according to ISO guide 35-2006, by the data in table 3, the time (moon) is represented with x, standard items are represented with y
Value, fitting it is in alignment, obtain linear equation be：Y=0.00088x+9.802, wherein, slope a=0.00088, intercept
B=9.802.
The standard deviation of straight line can be calculated by following formula：
Slope uncertainty is calculated with following formula：
Degree of freedom is n-2=5-2=3 and p=0.95 (95% confidence interval), and it is 3.2 to look into t values table.
Due to | a |<t2 0.05×s(a)Therefore slope is not notable, thus the influence to stability is not observed.Illustrate embodiment 1
" nicotine solution standard substance in isopropanol " under defined storage condition 14 months internal stabilities it is good.
(2) uncertainty evaluation
The uncertainty of nicotine content definite value result is made of three parts in " nicotine solution standard substance in isopropanol "：Mark
Uncertainty, the definite value of standard substance that uncertainty, the stability of standard substance of the uniformity introducing of quasi- substance introduce are (pure
Degree, quality, volume, preparation) measurement process introduce uncertainty.
Its relative uncertainty degree：
Statistical method refers to《Evaluation of uncertainty in measurement is with representing》(JJF 1059.1-2012), the reference of the numerical value revision of the convention《One
Grade standard substance》(JJG 1006-1994) and《Rules of rounding off for numerical values ＆ expression and judgement of limiting values》(GB/T 8170-
2008)。
The uncertainty that quality introduces：
(1) A classes uncertainty.Respectively to nicotine dosage duplicate measurements, result is respectively 19.7098,19.6941,
19.6862,19.7294,19.6941,19.6666,19.6941,19.7058g, be by Bessel Formula standard deviation
(2) B classes uncertainty.It tests the electronic balance used and is calibrated to 1. grade day through metering scientific research institute of Henan Province
Flat, minimum indexing is 0.0001g, and reading is secondary, and category is uniformly distributed, and is takenUncertainty caused by balance calibration
(3) Composite Seismogram.By the A classes of weighing, B classes uncertainty combination to weigh uncertainty,
(4) relative uncertainty degree is synthesized.
Purity introduces uncertainty：
Since nicotine purity is provided by standard items certificate, only there are one B class uncertainties.If purity causes
Standard uncertainty to be uniformly distributed, then the uncertainty that purity introduces is as shown in table 4.
4 nicotine purity of table introduces uncertainty
The uncertainty that volume introduces：Since the Temperature and Humidity Control in laboratory is preferable, we do not consider it to capacity
The influence of bottle volume expansion.It being full of, weighed by carrying out 8 scales to volumetric flask with water, obtained result is 1999.6,
1998.8,1998.0,2000.4,2000.4,2000.8,1997.6,1999.2mL.It is by Bessel Formula standard deviation
1.65mL, relative standard uncertainty 0.0008.
The uncertainty that uniformity introduces：Standard uncertainty (the S that uniformity introducesH) 1 is shown in Table, mean value is
9.80mg/mL, relative standard uncertainty 0.00235.
The uncertainty that long-time stability introduce：The standard uncertainty introduced steadily in the long term for 0.00399mg/mL (see
Table 3), the average value of long-time stability is 9.807mg/mL, relative standard uncertainty 0.00041.
Total synthesis relative standard uncertainty
Total synthesis relative standard uncertainty is calculated according to the following formula, and the result is shown in tables 5.
5 nicotine relative standard uncertainty component of table and synthesis
The combined standard uncertainty of nicotine
Total combined standard uncertainty is calculated according to the following formula, and the result is shown in tables 6.
The standard uncertainty of 6 nicotine of table
Nicotine expanded uncertainty
When fiducial probability is 95%, it is 2, the i.e. expanded uncertainty of nicotine to take spreading factor：
uP=k × uc(c)。
The result is shown in tables 7.
7 nicotine expanded uncertainty of table and the revision of the convention
To sum up, above-mentioned " nicotine solution standard substance in isopropanol " have it is uniform in material, property stablize, can be mass,
The features such as accurate definite value, uncertainty is small, magnitude tracing, can meet the requirement of national grade ii standard substance, so can make
It is used for standard substance.
Embodiment 2
The nicotine solution standard substance of the present embodiment, is made of nicotine purity rubric substance and water, the concentration of nicotine solution
For 9.80mg/mL.
The preparation method of the nicotine solution standard substance of the present embodiment, includes the following steps：
1) it weighs：The substance that the number for weighing 19.6000g State Standard Matter Research Centre is GBW (E) 100264 in
In 50mL beakers, adding in water makes its dissolving, and be transferred in 2000mL volumetric flasks；
2) it is quantitative：Small beaker is rinsed repeatedly with water, washing lotion is incorporated in volumetric flask, and is added water and mixed, and is settled to quarter
Degree, it is 9.80mg/mL to determine its actual concentrations；
3) it is the nicotine solution of a concentration of 9.80mg/mL is filling in the Brown Glass Brown glass bottles and jars only of 50mL, sealing.
Nicotine solution product carries out outer packing using black kraft paper bag, is stored in -18 DEG C of refrigerator-freezer.
The preparation method of embodiment 2 is repeated 10 times, and 400 bottles of nicotine solution products are obtained.The nicotine solution of the present embodiment
It is as follows as standard substance evaluation rubric：
(1) uniformity and stability test
The results are shown in Table 8 for uniformity testing.According to ISO guide 35-2006, statistical is carried out using F methods of inspection
Analysis, less than F0.05(14,30) show that significant difference is not present under 95% level of fiducial probability between sample, after packing
Nicotine solution standard substance sample is uniform.
8 uniformity testing result of table
Short-term stability experimental result is as shown in table 9.The result shows that the short-term stability of " nicotine solution standard substance in water "
Property examine t values be respectively less than critical value, thus illustrate embodiment 2 nicotine solution standard substance packaging can ensure meet transport
And to the general requirement of environment in transfer process.
Nicotine solution standard substance high-temperature stability inspection data in 9 water of table
Long-time stability experimental result is shown in Table 10.Due to | a |<t2 0.05×s(a), therefore slope is not notable, thus do not observe
Influence to stability.Illustrate " nicotine solution standard substance in water " sample 14 months internal stabilities under defined storage condition
Well.
Nicotine solution standard substance long-time stability data in 10 water of table
(2) it carries out nicotine relative standard uncertainty component according to the method for embodiment 1 and synthesis is assessed, concrete outcome is shown in
Table 11.
11 nicotine relative standard uncertainty component of table and synthesis
The combined standard uncertainty of nicotine
Total combined standard uncertainty is calculated according to the following formula, and the result is shown in tables 12.
The standard uncertainty of 12 nicotine of table
Nicotine expanded uncertainty
When fiducial probability is 95%, it is 2, the i.e. expanded uncertainty of nicotine to take spreading factor：
uP=k × uc(c)。
The result is shown in tables 13.
13 nicotine expanded uncertainty of table and the revision of the convention
To sum up, above-mentioned " nicotine solution standard substance in water " have it is uniform in material, property stablize, can be mass, accurately
The features such as definite value, uncertainty is small, magnitude tracing can meet the requirement of national grade ii standard substance, so mark can be used as
Quasi- substance migration.
Test example 1
The nicotine solution product of this test example Application Example 1 is as standard items for nicotine content in cigarette mainstream flue gas
Test.
According to《The measure gas chromatography of nicotine in Cigarette grain phase》Method specified in (GB/T 23355-2009)
Nicotine content in different nicotine amount cigarette mainstream flue gas is measured, the concentration range of serial nicotine standard solution is
0.02mg/mL-2.0mg/mL, the nicotine standard for the use of the nicotine solution product of embodiment 1, number being respectively GBW (E) 100264
The solution that substance and isopropanol are prepared makes standard working curve and same sample is tested, as a result as shown in table 14.
Nicotine content measurement result in 14 cigarette mainstream flue gas of table
As shown in Table 14, using the nicotine solution product of embodiment 1 with using national standard substance obtained
Testing result is consistent, it was demonstrated that the nicotine solution product can be used as measure of the standard items for nicotine content in cigarette mainstream flue gas.
Test example 2
The nicotine solution product of this test example Application Example 1 is as standard items for the survey of nicotine content in electronic cigarette liquid
Examination.
According to《The measure gas chromatography of electronic cigarette liquid nicotine, 1,2- propylene glycol and glycerine》(YQ-EL/T2-2017)
Specified in method the nicotine content in different nicotine amount electronic cigarette liquids is measured, the concentration model of serial nicotine standard solution
It encloses for 0.02mg/mL-2.0mg/mL, the nicotine for the use of the nicotine solution product of embodiment 1, number being respectively GBW (E) 100264
The solution that standard substance and isopropanol are prepared makes standard working curve and same sample is tested, as a result as shown in Table 15.
Nicotine content measurement result in 15 electronic cigarette liquid of table
As shown in Table 15, using the nicotine solution product of embodiment 1 with using national standard substance obtained
Testing result is consistent, it was demonstrated that the nicotine solution product can be used as measure of the standard items for nicotine content in electronic cigarette liquid.
Test example 3
The nicotine solution product of this test example Application Example 1 contains as standard items for heating the Nicotine from Cigarette that do not burn
The measure of amount.
The nicotine content not burnt to the heating of different brands, different taste in following manner in cigarette is measured：
The one end for heating smoke grenade of not burning is inserted into tobacco heating rod, and smoke grenade filter end is inserted into the trap containing cambridge filter
On；It opens tobacco heating stick switch to heat smoke grenade, be aspirated in heating process, while trap its aerosol；Again
To trap has the cambridge filter isopropyl alcohol extracting of aerosol, supernatant liquor is taken to be measured after extraction, and instrument during measure is set
Put parameter according to《The measure gas chromatography of nicotine in Cigarette grain phase》Condition specified in (GB/T 23355-2009)；
The concentration range of serial nicotine standard solution be 0.02mg/mL-2.0mg/mL, respectively using the nicotine solution product of embodiment 1,
The solution that the nicotine standard substance and isopropanol that number is GBW (E) 100264 are prepared makes standard working curve to same sample
It is tested, as a result as shown in table 16.
Table 16 heats Nicotine from Cigarette assay result of not burning
As shown in Table 16, using the nicotine solution product of embodiment 1 with using national standard substance obtained
Testing result is consistent, it was demonstrated that the nicotine solution product can be used as standard items for heat do not burn the survey of Nicotine from Cigarette content
It is fixed.
Test example 4
The nicotine solution product of this test example Application Example 2 contains as standard items for nicotine in tobacco and tobacco product
The measure of amount.
According to《Tobacco and the measure gas chromatography of tobacco product nicotine》Method specified in (YC/T 246-2008)
Nicotine content in different type tobacco and tobacco product is measured, the concentration range of serial nicotine standard solution is
0.02mg/mL-2.0mg/mL, the nicotine standard for the use of the nicotine solution product of embodiment 2, number being respectively GBW (E) 100264
The solution that substance and water are prepared makes standard working curve and same sample is tested, as a result as shown in table 17.
Nicotine content measurement result in 17 tobacco of table and tobacco product
As shown in Table 17, using the nicotine solution product of embodiment 2 with using national standard substance obtained
Testing result is consistent, it was demonstrated that the nicotine solution product can be used as survey of the standard items for nicotine content in tobacco and tobacco product
It is fixed.
Test example 5
The nicotine solution product of this test example Application Example 2 contains as standard items for nicotine in tobacco and tobacco product
The measure of amount.
According to《The measure of tobacco and tobacco product total alkaloid continuously flows (potassium rhodanide) method》(YC/T468-2013)
Specified in method the total alkaloid in different type tobacco and tobacco product is measured, serial nicotine standard solution it is dense
Ranging from 0.02mg/mL-2.0mg/mL is spent, the use of the nicotine solution product of embodiment 2, number is respectively GBW (E) 100264
The solution that nicotine standard substance and water are prepared makes standard working curve and same sample is tested, as a result as shown in table 18.
Total alkaloid content measurement result in 18 tobacco of table and tobacco product
As shown in Table 18, using the nicotine solution product of embodiment 2 with using national standard substance obtained
Testing result is consistent, it was demonstrated that the nicotine solution product can be used as standard items for total alkaloid content in tobacco and tobacco product
It measures.
Claims (9)
1. a kind of nicotine solution standard substance, which is characterized in that be made of nicotine purity rubric substance and solvent, solvent is isopropyl
Alcohol or water, a concentration of 5.00-9.80mg/mL of nicotine solution.
2. nicotine solution standard substance as described in claim 1, which is characterized in that the nicotine purity rubric substance is in country
The number in standard substance research center is GBW (E) 100264.
3. a kind of preparation method of nicotine solution standard substance as described in claim 1, which is characterized in that including following step
Suddenly：Nicotine purity rubric substance is dissolved in solvent, compound concentration is the nicotine solution of 5.00-9.80mg/mL.
4. the preparation method of nicotine solution standard substance as claimed in claim 3, which is characterized in that prepared nicotine is molten
Liquid is filling in Brown Glass Brown glass bottles and jars only, seals up to nicotine solution standard substance product.
5. the preparation method of nicotine solution standard substance as claimed in claim 4, which is characterized in that the nicotine solution standard
Physical product carries out outer packing using black kraft paper bag.
6. the preparation method of nicotine solution standard substance as claimed in claim 4, which is characterized in that the nicotine solution standard
Physical product is stored in -18 DEG C~-16 DEG C of environment.
7. a kind of nicotine solution standard substance as described in claim 1 nicotine or using in terms of nicotine in the test of compound as
The application of standard items.
8. the use as claimed in claim 7, which is characterized in that the measure of nicotine, electronics in applied to cigarette mainstream flue gas
The measure of nicotine in tobacco juice, heating do not burn Nicotine from Cigarette measure when, use the nicotine solution standard that solvent is isopropanol
Substance is standard items.
9. the use as claimed in claim 7, which is characterized in that the measure of nicotine, cigarette in applied to tobacco and tobacco product
The use of the nicotine solution standard substance that solvent is water is standard items in grass and tobacco product during the measure of vegetable soda.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711483723.1A CN108254233A (en) | 2017-12-29 | 2017-12-29 | A kind of nicotine solution standard substance and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711483723.1A CN108254233A (en) | 2017-12-29 | 2017-12-29 | A kind of nicotine solution standard substance and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108254233A true CN108254233A (en) | 2018-07-06 |
Family
ID=62725360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711483723.1A Pending CN108254233A (en) | 2017-12-29 | 2017-12-29 | A kind of nicotine solution standard substance and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108254233A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109738557A (en) * | 2018-12-21 | 2019-05-10 | 国家烟草质量监督检验中心 | A kind of preparation method of nicotine solution standard substance |
| CN109738556A (en) * | 2018-12-21 | 2019-05-10 | 国家烟草质量监督检验中心 | A kind of preparation method of nicotine standard substance and its nicotine standard substance of preparation |
| CN114200057A (en) * | 2021-12-14 | 2022-03-18 | 坛墨质检科技股份有限公司 | Preparation method of antibiotic medicine solid mixed standard substance capable of replacing standard solution |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0390419A2 (en) * | 1989-03-31 | 1990-10-03 | British-American Tobacco Company Limited | Improvements relating to smoking articles |
| CN102226786A (en) * | 2011-04-08 | 2011-10-26 | 红云红河烟草(集团)有限责任公司 | Method for accurately measuring free nicotine in main stream smoke of cigarette |
| CN102230924A (en) * | 2011-04-08 | 2011-11-02 | 红云红河烟草(集团)有限责任公司 | Method for measuring free nicotine in tobacco and tobacco products |
| CN102353739A (en) * | 2011-09-01 | 2012-02-15 | 湖北中烟工业有限责任公司 | Rapid detection method of nicotine in tobacco |
| CN102796076A (en) * | 2012-09-03 | 2012-11-28 | 国家烟草质量监督检验中心 | Preparation method for nicotine standard substance |
| CN104062385A (en) * | 2014-07-18 | 2014-09-24 | 云南中烟工业有限责任公司 | Method for detecting content of nicotine in smoke of electronic cigarette |
| CN106442501A (en) * | 2016-09-29 | 2017-02-22 | 福建中烟工业有限责任公司 | Detection method of nicotine content of cigarette liquid of electronic cigarette |
| CN106932462A (en) * | 2017-02-24 | 2017-07-07 | 云南中烟工业有限责任公司 | A kind of method of quick measure Determination of Nicotine Content of Tobacco |
-
2017
- 2017-12-29 CN CN201711483723.1A patent/CN108254233A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0390419A2 (en) * | 1989-03-31 | 1990-10-03 | British-American Tobacco Company Limited | Improvements relating to smoking articles |
| CN102226786A (en) * | 2011-04-08 | 2011-10-26 | 红云红河烟草(集团)有限责任公司 | Method for accurately measuring free nicotine in main stream smoke of cigarette |
| CN102230924A (en) * | 2011-04-08 | 2011-11-02 | 红云红河烟草(集团)有限责任公司 | Method for measuring free nicotine in tobacco and tobacco products |
| CN102353739A (en) * | 2011-09-01 | 2012-02-15 | 湖北中烟工业有限责任公司 | Rapid detection method of nicotine in tobacco |
| CN102796076A (en) * | 2012-09-03 | 2012-11-28 | 国家烟草质量监督检验中心 | Preparation method for nicotine standard substance |
| CN104062385A (en) * | 2014-07-18 | 2014-09-24 | 云南中烟工业有限责任公司 | Method for detecting content of nicotine in smoke of electronic cigarette |
| CN106442501A (en) * | 2016-09-29 | 2017-02-22 | 福建中烟工业有限责任公司 | Detection method of nicotine content of cigarette liquid of electronic cigarette |
| CN106932462A (en) * | 2017-02-24 | 2017-07-07 | 云南中烟工业有限责任公司 | A kind of method of quick measure Determination of Nicotine Content of Tobacco |
Non-Patent Citations (4)
| Title |
|---|
| I•森夏因 等: "《毒物分析方法学》", 28 February 1986, 群众出版社 * |
| 北京国质联企业管理中心: "《标准物质应用指南》", 31 December 2008, 中国计量出版社 * |
| 李中皓 等: "烟碱纯度标准物质的研制", 《烟草化学》 * |
| 王奕 等: "紫外分光光度法测定电子烟油中的烟碱", 《轻工科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109738557A (en) * | 2018-12-21 | 2019-05-10 | 国家烟草质量监督检验中心 | A kind of preparation method of nicotine solution standard substance |
| CN109738556A (en) * | 2018-12-21 | 2019-05-10 | 国家烟草质量监督检验中心 | A kind of preparation method of nicotine standard substance and its nicotine standard substance of preparation |
| CN114200057A (en) * | 2021-12-14 | 2022-03-18 | 坛墨质检科技股份有限公司 | Preparation method of antibiotic medicine solid mixed standard substance capable of replacing standard solution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108254233A (en) | A kind of nicotine solution standard substance and its preparation method and application | |
| CN102128906A (en) | Method for detecting volatile organic compound in cigarette filter | |
| CN110487612A (en) | A kind of schizandrin A purity rubric substance and the preparation method and application thereof | |
| CN104111264A (en) | Methods for quickly detecting content of heavy metal elements in rice and evaluating comprehensive pollution index of heavy metal elements | |
| CN108827998A (en) | A method of volatile substances purity is accurately measured using quantitative nuclear magnetic resonance spectroscopy | |
| CN101290323A (en) | Cow milk and milk powder nitrite qualitative checking method and reagent kit | |
| CN107561182A (en) | Volatility and the detection method of semi-volatile organic compounds in cigarette mainstream flue gas | |
| CN110174458A (en) | The detection method that lead and total arsenic measure simultaneously in a kind of formulated food additive | |
| JP6804594B2 (en) | How to make a standard product | |
| de Krom et al. | Primary mercury gas standard for the calibration of mercury measurements | |
| CN102305824B (en) | Method for accurately determining serum potassium | |
| CN107843640A (en) | The assessment method of 20 kinds of constituent content uncertainties in honeysuckle | |
| CN106248839A (en) | The standard substance of compound fragrant hydrocarbon detection in workplace air | |
| CN107894414B (en) | Method for determining release amount of heavy metal elements in buccal cigarette | |
| CN106168587A (en) | A kind of measure the method for iron content in fireworks and firecrackers firework medicament | |
| CN108982751A (en) | A method of measurement Rice oil fatty acid value | |
| CN107991375A (en) | The kit of iodine in a kind of measure human urine | |
| CN103063606A (en) | Refractive index solution standard substance and preparation method thereof | |
| CN109738556A (en) | A kind of preparation method of nicotine standard substance and its nicotine standard substance of preparation | |
| CN109406429A (en) | A method of the atomic absorption spectroscopy determination content of heavy metal lead without matrix modifier | |
| Hughes | Testing of glass volumetric apparatus | |
| Li et al. | Real-Time Monitoring of Nicotine Release Behavior from Smokeless Tobacco (Snus) Based on Fiber Optic Sensing Technology | |
| Zhang et al. | Determination of the total ginsenosides in ginseng using the UV spectrophotometer and evaluation of the measurement uncertainty | |
| CN115308343B (en) | Acid red solution standard substance and preparation method and application thereof | |
| Rusdiana et al. | Determination of methampyrone levels using Thin Layer Chromatography and UV Spectrophotometry method in Gout herbal medicine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180706 |