CN108251327A - Applications of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium - Google Patents
Applications of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium Download PDFInfo
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Abstract
The invention discloses applications of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium.Thermophilic Stenotrophomonas DSM14405 of the present invention survives in slight alkali environment, and for being trivalent chromium by hexavalent chrome reduction, trivalent chromium is combined with hydroxyl generates chromium hydroxide precipitation.Thermophilic Stenotrophomonas DSM14405 is to hexavalent chrome reduction speed by the present invention, 32 hours reduction rates of Cr VI of substantially 50mg/L are up to 100%, and it is higher to the tolerable concentration of Cr VI, and when hexavalent chromium concentration reaches 200mg/L, thalline can also be grown and 48 hours reduction rates are up to 47.6%.
Description
Technical field
The present invention relates to biotechnologies, and in particular to thermophilic Stenotrophomonas (Stenotrophomonas
Rhizophila) applications of the DSM14405 in reduction of hexavalent chromium.
Background technology
Chromic salts is mainly used for the processing of metal and process hides and plating etc. industry.It recycles in order to prevent in the industrial production
Corrosion of the water to various equipment, also frequently adds in chromate.Recently as China's rapid economic development, chromium in industrial production
Salt dosage is more and more, and it is one of current heavy industries pollution sources most important in the world that it, which produces waste residue,.So work
Pollution of chromium in industry waste water and exhaust gas is also increasingly severe.Chromium mainly has two kinds of forms of trivalent chromium and Cr VI in the environment, and two
The chemical behavior and toxicity of kind form are of different sizes.Wherein more absorption are deposited on bed mud to trivalent chromium in solid matter in water
In, harm is smaller；And how soluble in water Cr VI is, and more stable, to human toxicity and environmental hazard bigger.So how
Cr VI in waste water is converted into easily precipitation and endangers smaller trivalent chromium, is the emphasis that current pollution of chromium is administered.
During pollution of chromium is administered, method chemically and physically is likely to occur administering to be not thorough or generate secondary pollution
It may.So microbial treatment method is method more popular at present, and with treatment cost is low, will not bring secondary dirt
The advantages of dye.
Invention content
In view of this, it is an object of the invention to propose thermophilic Stenotrophomonas DSM14405 answering in reduction of hexavalent chromium
With thermophilic Stenotrophomonas DSM14405 of the invention can be resistant to higher concentration Cr VI and can be three by hexavalent chrome reduction
Valency chromium reduces the content of 6-valence Cr ions in soil and water body, reduces the danger that people take in Cr VI, reduces suction of the plant to chromium
It receives, enriches the technical method of pollution of chromium microorganism remediation.
Based on above-mentioned purpose, applications of the thermophilic Stenotrophomonas DSM14405 provided by the invention in reduction of hexavalent chromium.
In some embodiments of the invention, the thermophilic Stenotrophomonas DSM14405 survives in slight alkali environment,
For being trivalent chromium by hexavalent chrome reduction, trivalent chromium is combined with hydroxyl generates chromium hydroxide precipitation.
Cr VI in water can be reduced into low toxicity by the thermophilic Stenotrophomonas DSM14405 of the present invention by enzymatic reaction
Trivalent chromium, which more preferably survives in the environment of meta-alkalescence, so the trivalent chromium that reduction reaction generates is combined with hydroxyl
The toxicity of pollution of chromium in environment can further be reduced by generating chromium hydroxide precipitation.
In some embodiments of the invention, the thermophilic Stenotrophomonas DSM14405 is used for hexavalent chromium polluted waste water
In processing.
The present invention thermophilic Stenotrophomonas DSM14405 in repairing hexavalent chromium polluted soil, can crops (such as
Rice, wheat, veterinary antibiotics etc.) thermophilic Stenotrophomonas DSM14405 is applied in planting process, it can effectively reduce by six in soil
The content of valency chromium reduces absorption of the plant to chromium, and then chromium is prevented to pass through the edible organ contaminated food of plant.
In some embodiments of the invention, by the thermophilic Stenotrophomonas DSM14405 for repairing hexavalent chromium polluted
In soil.
It, can be by thermophilic widow when the thermophilic Stenotrophomonas DSM14405 of the present invention is applied in hexavalent chromium polluted wastewater treatment
It supports monad DSM14405 to be inoculated in hexavalent chromium polluted waste water, hexavalent chrome reduction can be by thermophilic Stenotrophomonas DSM14405
Trivalent chromium reduces the content of Cr VI, realizes the reparation of pollution of chromium waste water.
In some embodiments of the invention, cultures of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium
Condition is：Temperature be 24~33 DEG C, pH be 6.0~8.0, rotating speed be 120~210rpm/min, Cr VI a concentration of 10~
100mg/L。
In some embodiments of the invention, cultures of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium
Condition is：Temperature is 30 DEG C, pH 7.5, rotating speed 180rpm/min, a concentration of 50mg/L of Cr VI.
The thermophilic Stenotrophomonas DSM14405 of the present invention, is purchased in American Type Culture collection, preservation
Number：ATCC BAA-473T。
The thermophilic Stenotrophomonas DSM14405, microorganism is in rod-short, dispersed arrangement, colony diameter about 2-
3mm, bacterium colony are circle, and yellow, surface is smooth, neat in edge.Bacterium is led to without pod membrane, no gemma, Gram-negative, bacterium
Fragmentation is crossed to be bred.The bacterium is heterotrophic bacterium, and oxygen is needed in growth course, does not need to sunlight, contacts enzyme reaction
The positive, oxydase reaction are negative.The optimum growth temperature of the bacterium is 30 DEG C or so, and environmental optima pH is 7.0 or so.The bacterium
Addition growth factor and other nutriments are not needed in growth course.
Compared with prior art, the invention has the advantages that：
Thermophilic Stenotrophomonas DSM14405 is to hexavalent chrome reduction speed, the substantially Cr VI of 50mg/L by the present invention
32 hours reduction rates are up to 100%, and it is higher to the tolerable concentration of Cr VI, when hexavalent chromium concentration reaches 200mg/L
Thalline can also be grown and 48 hours reduction rates are up to 47.6%, so thermophilic Stenotrophomonas DSM14405 can be used for pollution of chromium
In processing.
Description of the drawings
Fig. 1 is thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Growth in the range of a concentration of 0-1000mg/L is bent
Line compares figure；
Fig. 2 is thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Cr in the range of a concentration of 0-1000mg/L6+Also
Former rate compares figure；
Fig. 3 is thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Growth curve in the range of a concentration of 0-200mg/L
Compare figure；
Fig. 4 is thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Cr in the range of a concentration of 0-200mg/L6+Reduction
Rate compares figure；
Fig. 5 is for different initial pH to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Life during a concentration of 50mg/L
Long curve influences figure；
Fig. 6 is for different initial pH to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+Cr during a concentration of 50mg/L6 +Reduction rate influences figure；
Fig. 7 is for different shaking speeds to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+During a concentration of 50mg/L
Growth curve influences figure；
Fig. 8 is for different shaking speeds to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+During a concentration of 50mg/L
Cr6+Reduction rate influences figure；
Fig. 9 is for different cultivation temperatures to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+During a concentration of 50mg/L
Growth curve influences figure；
Figure 10 is for different cultivation temperatures to thermophilic Stenotrophomonas DSM14405 in culture medium C r6+During a concentration of 50mg/L
Cr6+Reduction rate influences figure；
Figure 11 is thermophilic Stenotrophomonas DSM14405 after various training systerns in culture medium C r6+A concentration of 50mg/L
When growth curve chart and Cr6+Situation map is restored, compares the Cr not add in bacterium solution6+Concentration changes.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Attached drawing, the present invention is described in more detail.
The existence form of Cr VI Cr (VI) described in following embodiments is potassium bichromate (K2Cr2O7)。
Embodiment 1：The discovery of the hexavalent chrome reduction performance of thermophilic Stenotrophomonas DSM14405
(1) bacterium source：Thermophilic Stenotrophomonas DSM14405 is purchased in American Type Culture collection, is protected
Hide number：ATCC BAA-473T。
(2) culture medium
Pancreas peptone soybean broth culture medium (TSB)：Tryptone 17g/L, soy peptone 3g/L, glucose 2.5g/
L, sodium chloride 5.0g/L, dipotassium hydrogen phosphate 2.5g/L, pH7.3 ± 0.2.
Tryptose soya agar culture medium (TSA)：Tryptone 17g/L, soy peptone 3g/L, glucose 2.5g/
L, sodium chloride 5.0g/L, dipotassium hydrogen phosphate 2.5g/L, agar 15g/L, pH7.3 ± 0.2.
The culture medium of Cr containing various concentration (VI)：With the Cr (VI) of 5000mg/L for mother liquor, phase is added in TSB culture mediums
The mother liquor that should be measured is configured to Cr (VI) culture medium of different final concentrations.
(3) whether thermophilic Stenotrophomonas DSM14405 has the identification of reduction Cr (VI) ability and its upgrowth situation
1. prepared by the seed liquor of thermophilic Stenotrophomonas DSM14405
With oese from the glycerol stock liquid pipe of defrosting thermophilic Stenotrophomonas DSM14405 to TSA culture medium of one ring of picking
Scribing line is carried out to be separately cultured.After the bacterium colony that 48h tablets grow suitable size, one colony inoculation of picking to TSB culture solutions
In, in 180rpm/min, 30 DEG C of cultures for 24 hours, as seed liquor, it is short-term spare to be placed in 4 DEG C of preservations.
2. the growth of thermophilic Stenotrophomonas DSM14405 is determined with Cr (VI) reducing power
It is respectively 0mg/L, 10mg/L, 20mg/L, 50mg/L, 100mg/L, 500mg/L, 1000mg/L that (VI) containing Cr, which is configured,
TSB culture mediums.The seed liquor of 1% (volume fraction) is added in thereto, is placed in 180rpm/min, is cultivated in 30 DEG C of shaking table,
Every strain density (OD600) of survey and Cr (VI) concentration for 24 hours, until 72h is cultivated.Growth curve result figure as shown in Figure 1,
After culture for 24 hours, the nectar angle value in the culture medium of each Cr (VI) concentration has kept stable substantially, wherein Cr (VI)
Bacterium growth in 100mg/L and following culture medium is smaller with control group difference, can normal growth.And Cr (VI) 500mg/
Bacterium in the culture medium of the L and 1000mg/L OD600 values in the 72h of culture are not above 1 always, it is possible that being that Cr (VI) is dense
Spend height, it is suppressed that the normal growth of bacterium.
For Cr (VI) reducing power, the results are shown in Figure 2, after culture for 24 hours Cr (VI) initial concentration for 10mg/L and
Reduction rate in the culture medium of 20mg/L is up to 100%；The culture medium reduction rate that Cr (VI) initial concentration is 50mg/L is
92.4%.During to culture 48h, Cr (VI) initial concentration is that the reduction rate in the culture medium of 50mg/L and 100mg/L also reaches
100%.But Cr (VI) initial concentration is in the culture medium of 500mg/L and 1000mg/L within the 72h times of entire culture detection
Reduction rate maintain reduced levels always.It can to sum up obtain, in the present embodiment, thermophilic DSM14405 pairs of Stenotrophomonas
Cr (VI) concentration 100mg/L and it is following when growing state and reduction effect it is preferable.
Embodiment 2：The most suitable hexavalent chrome reduction concentration of thermophilic Stenotrophomonas DSM14405 determines
According to embodiment 1 it was determined that thermophilic Stenotrophomonas DSM14405 to Cr (VI) concentration 100mg/L and it is following when
Growing state and reduction effect it is preferable.In the present embodiment, configuration (VI) containing Cr be respectively 0mg/L, 50mg/L, 100mg/L,
The seed liquor of thermophilic Stenotrophomonas DSM14405 is inoculated in above-mentioned TSB culture mediums by the TSB culture mediums of 200mg/L, according to
Growing states of the thermophilic Stenotrophomonas DSM14405 in above-mentioned TSB culture mediums and reduction effect determine thermophilic oligotrophy unit cell
The most suitable hexavalent chrome reduction concentration of bacterium DSM14405.It is as follows：
1. prepared by the seed liquor of thermophilic Stenotrophomonas DSM14405
The bacterium solution cultivated for 24 hours is taken, is inoculated into TSB culture solutions with the amount of 1% (volume fraction), in 180rpm/min,
30 DEG C of cultures, the continuous growth pattern for monitoring bacteria biomass in culture solution, until culture solution OD600 values are to 1.0, as kind
It is short-term spare to be placed in 4 DEG C of preservations for sub- liquid.
2. the growth of thermophilic Stenotrophomonas DSM14405 is determined with most suitable hexavalent chrome reduction concentration range
Configuration (VI) containing Cr is respectively the TSB culture mediums of 0mg/L, 50mg/L, 100mg/L, 200mg/L.It adds in thereto
1% seed liquor is placed in 180rpm/min, is cultivated in 30 DEG C of shaking table, and a strain density (OD600) and Cr (VI) are surveyed every 8h
Concentration, until 48h is cultivated.After growth curve result figure is as shown in figure 3, cultivate 48h, the training of Cr (VI) a concentration of 50mg/L
It is more normal to support bacterium stand density value in base.In the culture medium of a concentration of 100mg/L and 200mg/L of Cr (VI) bacterium growth by
Inhibit.
For Cr (VI) reducing power, the results are shown in Figure 4, the training that Cr (VI) initial concentration is 50mg/L after culture 32h
The reduction rate in base is supported up to 100%；Cr (VI) initial concentration is that the culture medium reduction rate of 100mg/L and 200mg/L is being cultivated
There are no complete reduction during 48h.It can to sum up obtain, in the present embodiment, thermophilic Stenotrophomonas DSM14405 is Cr (VI)
Growing state and reduction effect during concentration 50mg/L is optimal.
Embodiment 3：Influence of the environmental condition to the hexavalent chrome reduction ability of thermophilic Stenotrophomonas DSM14405
From embodiment 2, it is known that during Cr (VI) concentration 50mg/L, the growth of thermophilic Stenotrophomonas DSM14405 and sexavalence
Chromium reduction effect is preferable, so the present embodiment is when doing training systern, using Cr (VI) a concentration of 50mg/L as research concentration.
(1) influence of initial pH value
In Cr (VI) concentration 50mg/L, 30 DEG C of temperature, during rotating speed 180rpm/min, adjust TSB medium pHs for 6.0,
6.5th, 7.0,7.5,8.0 5 levels.The seed liquor that OD600 values are 1.0 is added to by 1% amount in culture medium, is cultivated
36h surveys an OD600 value and Cr (VI) concentration per 12h.Initial pH value is to the shadow of the growth of thermophilic Stenotrophomonas DSM14405
It rings as shown in figure 5, when pH is 6.0 and 6.5, thermophilic Stenotrophomonas DSM14405 is not grown substantially in 36h；PH7.0 is relatively slow
The stagnant time is longer, but nectar angle value can reach little with the difference of pH7.5 in 36h；In the culture medium of pH7.5 and pH8.0
Nectar angle value increases the level for comparatively fast, belonging to normal growth in culture, and pH7.5 is compared in nectar angle value growth during Ph8.0
When growth faster, culture to 36h when nectar angle value higher.
Influence of the initial pH value to reduction Cr (VI) ability of thermophilic Stenotrophomonas DSM14405 is as shown in fig. 6, in 36h
When, the group of pH7.5 and pH8.0 can reach reduction rate 100%；The group reduction rate of pH7.0 is higher, but not up to 100%；pH6.0
It is relatively low with the group reduction rate of pH6.5.And the group of pH7.5 restores the speed of Cr (VI) than pH8.0's within the complete period of detection
Soon, and the group of pH7.5 in detection cycle increment be less than pH8.0 group, the substance of consumption is also fewer, so comparatively
Initial pH7.5 restores Cr (VI) optimal pH for thermophilic Stenotrophomonas DSM14405.
(2) influence of shaking speed
In Cr (VI) concentration 50mg/L, 30 DEG C of temperature, culture medium starting pH is pH value of TSB culture mediums itself without adjusting
Section, adjusting shaking speed are tetra- levels of 120rpm/min, 150rpm/min, 180rpm/min, 210rpm/min.By OD600
The seed liquor being worth for 1.0 is added to by the amount of 1% (volume fraction) in TSB culture mediums, cultivates 36h, and an OD600 is surveyed per 12h
Value and Cr (VI) concentration.Influence of the shaking speed to the growth of thermophilic Stenotrophomonas DSM14405 is as shown in fig. 7, four rotating speeds
Under bacterium growing state it is all relatively good, but the group growing state of rotating speed 180rpm/min and 210rpm/min are two groups relatively another
Better.
Influence of the shaking speed to reduction Cr (VI) ability of thermophilic Stenotrophomonas DSM14405 is as shown in figure 8, in 12h
When, the reduction rate difference of four processing groups is little, and the group reduction rate of shaking speed 180rpm/min is slightly higher；The number measured when for 24 hours
It is substantially higher, and rotating speed 210rpm/ according to another two groups of the group reduction rate ratio that can be seen that rotating speed 180rpm/min and 210rpm/min
The group reduction rate of min is more little higher than the group of 180rpm/min；In 36h, rotating speed 180rpm/min and 210rpm/min
The reduction rate of two groups has all reached 100%, and another two groups do not restore Cr (VI) completely, so comparatively shaking speed
180rpm/min and 210rpm/min is more suitable for Cr (VI) reduction than another two rotating speed.And in rotating speed 180rpm/min and
In the comparison of 210rpm/min, although the increment of organism of rotating speed 210rpm/min is more than 180rpm/min, two turn
Cr (VI) reduction rate difference under speed is not obvious.To sum up, rotating speed 180rpm/min is small in bacterium increment, and depleting substance is few, and
And shaking speed is relatively low, in the case that power consumption also lacks, Cr (VI) reduction rates are close with rotating speed 210rpm/min's.So
With reference to the feasibility and cost consideration in actual production, determine that thermophilic the best of Stenotrophomonas DSM14405 reduction Cr (VI) turns
Speed is 180rpm/min.
(3) influence of cultivation temperature
In Cr (VI) concentration 50mg/L, rotating speed 180rpm/min, culture medium starting pH for TSB culture mediums pH value itself not into
Row is adjusted, and it is 24 DEG C, 27 DEG C, 30 DEG C, 33 DEG C of four levels to adjust cultivation temperature.The seed liquor that OD600 values are 1.0 is pressed 1%
The amount of (volume fraction) is added in culture medium, cultivates 36h, and an OD600 value and Cr (VI) concentration are surveyed per 12h.Cultivation temperature
Influence to the growth of thermophilic Stenotrophomonas DSM14405 is as shown in figure 9, bacterium growing state all phases under four cultivation temperatures
To good, but the group growing state of 30 DEG C of cultivation temperature is relatively better；What the group that 24 DEG C of cultivation temperature was measured in 36h
Nectar angle value increases suddenly is not excluded for abnormal possibility.
Influence of the cultivation temperature to reduction Cr (VI) ability of thermophilic Stenotrophomonas DSM14405 is as shown in Figure 10, four
The reduction rate of processing group is more close, but when cultivation temperature is 30 DEG C, reduction rate with respect to other or height a bit, and is being trained
It supports group Cr (VI) reduction rate for there was only 30 DEG C of cultivation temperature during 36h and has reached 100%.To sum up, thermophilic Stenotrophomonas is determined
The optimum temperature of DSM14405 reduction Cr (VI) is 30 DEG C.
By embodiment 1-3 it is found that best culture items of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium
Part is：Rotating speed 180rpm/min originates pH7.5,30 DEG C of cultivation temperature, Cr (VI) concentration 50mg/L, in the optimal culture condition
Under, thermophilic Stenotrophomonas DSM14405 is cultivated, obtains growth curve and Cr (VI) reduction result such as Figure 11, wherein control is
Cr (VI) reduction rate of bacterium is not added in the culture medium of Cr (VI) concentration 50mg/L.It can be seen from fig. 11 that 28 after optimization
Hour, the Cr (VI) of 50mg/L can be just completely reduced, faster than original 32 hours, in the blank control without adding bacterium
Reduction rate can only achieve 10% or so.It is because Cr (VI) is to compare oxygen that plus in the blank control of bacterium Cr (VI), which can not restored, yet
Gas oxidant also eager to excel in whatever one does can just aoxidize the organic matter in culture medium at normal temperatures, but oxidation rate is slower.Institute
Can to sum up determine that thermophilic Stenotrophomonas DSM14405 is the reduction rate that can promote Cr (VI), make Cr (VI) can
Quickly it is reduced into Cr (III).
In conclusion thermophilic Stenotrophomonas DSM14405 of the present invention is to hexavalent chrome reduction speed, substantially 50mg/
32 hours reduction rates of Cr VI of L are up to 100%, and it is higher to the tolerable concentration of Cr VI, reaches in hexavalent chromium concentration
Thalline can also be grown during 200mg/L and 48 hours reduction rates are up to 47.6%, so thermophilic Stenotrophomonas DSM14405 can
In being handled for pollution of chromium.
Those of ordinary skills in the art should understand that：The discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure is limited to these examples (including claim)；Under the thinking of the present invention, above example
Or it can also be combined between the technical characteristic in different embodiments, and there is different aspect present invention as described above
Many other variations, in order to it is concise they do not provided in details.Therefore, all within the spirits and principles of the present invention,
Any omission for being made, modification, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (6)
1. applications of the thermophilic Stenotrophomonas DSM14405 in reduction of hexavalent chromium.
2. application according to claim 1, which is characterized in that the thermophilic Stenotrophomonas DSM14405 is in meta-alkalescence ring
It survives in border, for being trivalent chromium by hexavalent chrome reduction, trivalent chromium is combined with hydroxyl generates chromium hydroxide precipitation.
3. application according to claim 1, which is characterized in that the thermophilic Stenotrophomonas DSM14405 is used for sexavalence
In pollution of chromium wastewater treatment.
4. application according to claim 1, which is characterized in that be used to repair by the thermophilic Stenotrophomonas DSM14405
In hexavalent chromium polluted soil.
5. application according to claim 1, which is characterized in that the thermophilic Stenotrophomonas DSM14405 is in reduction sexavalence
Condition of culture during chromium is：Temperature be 24~33 DEG C, pH be 6.0~8.0, rotating speed be 120~210rpm/min, Cr VI it is dense
It spends for 10~100mg/L.
6. application according to claim 5, which is characterized in that the thermophilic Stenotrophomonas DSM14405 is in reduction sexavalence
Condition of culture during chromium is：Temperature is 30 DEG C, pH 7.5, rotating speed 180rpm/min, a concentration of 50mg/L of Cr VI.
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| CN110551652A (en) * | 2019-08-28 | 2019-12-10 | 中国科学院生态环境研究中心 | Compound microbial agent and preparation method and application thereof |
| CN110643551A (en) * | 2019-11-18 | 2020-01-03 | 吉林大学 | Stenotrophomonas rhizophila S11 for preventing and treating rice blast and application thereof |
| CN111533585A (en) * | 2020-04-22 | 2020-08-14 | 中国科学院生态环境研究中心 | Application of stenotrophomonas rhizophila in improvement of rhizosphere soil and promotion of plant growth |
| CN112592850A (en) * | 2020-12-08 | 2021-04-02 | 北京农业生物技术研究中心 | Stenotrophomonas for promoting growth and development of lily and/or antagonizing lily pathogenic bacteria and application thereof |
| WO2021114784A1 (en) * | 2019-12-11 | 2021-06-17 | 山东科技大学 | Strain capable of tolerating high concentration of chromium, and microbial agent used for hexavalent chromium pollution control |
| CN113957014A (en) * | 2021-11-22 | 2022-01-21 | 辽宁中博生态环境技术有限公司 | Functional strain with polyhalogenated hydrocarbon degradation and chromium (VI) reduction activities and application thereof |
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| CN115287241A (en) * | 2022-09-15 | 2022-11-04 | 南昌航空大学 | Modified hexavalent chromium reducing bacteria and preparation method and application thereof |
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| WO2021114784A1 (en) * | 2019-12-11 | 2021-06-17 | 山东科技大学 | Strain capable of tolerating high concentration of chromium, and microbial agent used for hexavalent chromium pollution control |
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| CN115287241A (en) * | 2022-09-15 | 2022-11-04 | 南昌航空大学 | Modified hexavalent chromium reducing bacteria and preparation method and application thereof |
| CN115805232A (en) * | 2022-11-29 | 2023-03-17 | 西南科技大学 | Application method for efficiently treating hexavalent chromium contaminated soil by using encapsulated bacteria |
| CN115805232B (en) * | 2022-11-29 | 2023-10-20 | 西南科技大学 | Application method of encapsulated bacteria for efficiently treating hexavalent chromium polluted soil |
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