CN108251316A - The method of composite bacteria agent, feed or additive and aflatoxin degradation - Google Patents
The method of composite bacteria agent, feed or additive and aflatoxin degradation Download PDFInfo
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Abstract
The present invention relates to microorganism fields, and in particular, to the method for composite bacteria agent, feed or additive and aflatoxin degradation.The composite bacteria agent of the present invention, including saccharomycete and Penicillium notatum, the saccharomycete is salt tolerance candidiasis and/or methamidophos, and the Penicillium notatum is Penicillium griseofulvum and/or penicillium chrysogenum.Particular kind of saccharomycete and Penicillium notatum are combined use by the present invention, can effectively aflatoxin degradation, the stronger aflatoxin B1 of the carcinogenicity that can especially degrade.Saccharomycete and Penicillium notatum cooperation are creatively used to prepare composite bacteria agent in the present invention, which can easily handle or be added in Feed Material, so as to reduce the content of aspergillus flavus in feed, be conducive to raise the health of animal.
Description
Technical field
The present invention relates to microorganism fields, and in particular, to composite bacteria agent, feed or additive and degrading aspergillus flavus poison
The method of element.
Background technology
Mycotoxin is a kind of secondary metabolites that Toxigenic fungi generates in the process in harm, mainly includes aspergillus flavus poison
Element, deoxynivalenol (also known as vomitoxin, DON), zearalenone (ZEN) and fumonisin (FUM), reddish brown song
Mould toxin and T-2 toxin etc..
In recent years, frequently occurring due to China's extreme climate results in head blight to endanger wheat, based on corn
It is very popular, mycetogenetic fumonisin (FUM), ochratoxin and T-2 pollutions aggravate, and pollute exceeded wheat and corn not
It is disconnected to increase.The safe utilization of the exceeded grain of toxin is studied, can not only protect the kind grain interests of China peasant, while prevent inflow entrance
Safeguard that national food Safety of Food Quality is extremely urgent in grain market.
Aflatoxin (AFT) is the similar compound of a kind of chemical mechanical, is the derivative of dihydrofuran cumarin.
Aflatoxin is by aspergillus flavus (Aspergillus flavus) and aspergillus parasiticus (Aspergillus parasiticus) etc.
The secondary metabolite of generation, is primarily present in soil, animals and plants, in various nuts, particularly peanut and walnut.In soybean,
Aflatoxin is also frequently found in the products such as paddy, corn, macaroni, flavouring, milk, dairy produce, edible oil.Aspergillus flavus
Toxin especially aflatoxin B1 has stronger carcinogenesis, and therefore, there are sternly its content in food, feed in various countries
The limitation standard of lattice.
At present there are many kinds of the measures of removal aflatoxin, mainly including physical method, chemical method, biological method
And enzymatic isolation method.Physical method mainly has (1) to select mould grain, and milling processing is washed by rubbing with the hands repeatedly；(2) absorption method：Common adsorbent has
Zeolite, alukalin, activated carbon etc., when selecting endotoxin adsorbent, on the one hand it should be noted that adsorption capacity must have laboratory
And the dual data of zoopery can prove effectively, on the other hand consider the height adsorption capacity of adsorbent.(3) radiation treatment：
Aflatoxin irradiates unstable under ultraviolet light, can use detoxification under ultraviolet light.Chemical method has (1) alkali process method：It is yellow
The lactonic ring of aspertoxin can be readily destroyed by base, and form cumarin sodium salt；(2) organic solvent extractionprocess：Aflatoxin is soluble in
Organic solvent can extract separation, detoxification with organic solvent；(3) oxidation removal method：Bleaching powder, chlorine, hydrogen peroxide, ozone
Wait oxidants can be rapidly by aflatoxin oxidation removal；(4) Chinese herbal medicine detoxification：China is found in the fruit of a cubeb litsea tree for the first time within 1976
Volatile oil can thoroughly remove aflatoxin in food.Certain ingredients in volatile oil occur anti-with aflatoxin
It answers, changes the structure of toxin, so as to achieve the purpose that detoxification.Biological method is to reach the yellow song of removal by the effect of microorganism
The purpose of mould toxin.Enzymatic isolation method is to achieve the purpose that remove aflatoxin using the specificity of enzyme.
However the above method need to be improved to the removal effect of aflatoxin, therefore can there is an urgent need for developing one kind
Energy conservation and environmental protection and efficient degradation aflatoxin preparation and method.
Invention content
The defects of the purpose of the invention is to overcome in the prior art, provides a kind of degradation efficiency higher compound bacteria
The method of agent, feed or additive and aflatoxin degradation.
To achieve these goals, in a first aspect, the present invention provides a kind of composite bacteria agent, which includes yeast
Bacterium and Penicillium notatum, the saccharomycete are salt tolerance candidiasis (Candida versatilis) and/or methamidophos
(Zygosaccharomyces rouxii), the Penicillium notatum for Penicillium griseofulvum (Penicillium griseofulvum) and/
Or penicillium chrysogenum (Penicillium Chrysogenum).
Second aspect, the present invention provides application of the above-mentioned composite bacteria agent in aflatoxin degradation.
The third aspect the present invention provides a kind of feed or additive, contains above-mentioned compound bacteria in the feed or additive
Agent.
Fourth aspect, the present invention provides a kind of method of aflatoxin degradation, this method includes：To pending sample
Middle addition composite bacteria agent or additive, carry out haptoreaction 1-96h at neutral salt solution, 25-37 DEG C, wherein, it is described compound
Microbial inoculum is above-mentioned composite bacteria agent or above-mentioned additive.
In the present invention, saccharomycete and Penicillium notatum are combined use, can effectively aflatoxin degradation, especially
The stronger aflatoxin B1 of the carcinogenicity that can degrade.There is no be used cooperatively to drop the prior art about saccharomycete and Penicillium notatum
The report of aflatoxin is solved, the present inventor has found under study for action, and saccharomycete or Penicillium notatum, effect is used alone
It is poor that the two is relatively used in combination.Therefore, saccharomycete and Penicillium notatum cooperation are creatively used to prepare composite bacteria agent in the present invention, it should
Composite bacteria agent can be handled easily or is added in Feed Material, so as to reduce the content of aspergillus flavus in feed, be had
Conducive to the health of raising animal.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Specific embodiment
The specific embodiment of the present invention is described in detail below.It is it should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
The endpoint of disclosed range and any value are not limited to the accurate range or value herein, these ranges or
Value should be understood to comprising the value close to these ranges or value.For numberical range, between the endpoint value of each range, respectively
It between the endpoint value of a range and individual point value and can be individually combined with each other between point value and obtain one or more
New numberical range, these numberical ranges should be considered as specific open herein.
In a first aspect, the present invention provides a kind of composite bacteria agent, which includes saccharomycete and Penicillium notatum, the ferment
Female bacterium is salt tolerance candidiasis (Candida versatilis) and/or methamidophos (Zygosaccharomyces
Rouxii), the Penicillium notatum is Penicillium griseofulvum (Penicillium griseofulvum) and/or penicillium chrysogenum
(Penicillium Chrysogenum)。
Herein it should be noted that each microorganism contained in composite bacteria agent of the present invention may be either each microorganism
Viable bacteria body or the dead thalline of each microorganism are, it is preferable to use the viable bacteria body of each microorganism.
Composite bacteria agent according to the present invention, also containing the medium component for being useful for culture yeasts bacterium.Wherein, saccharomycete is trained
Foster base can be the various conventional culture mediums in this field, such as can be YEB culture mediums, and the composition of YEB culture mediums includes：Yeast
Extract 1g/L, peptone 5g/L, beef extract 5g/L, sucrose 5g/L, MgSO42mmol/L, pH value 7.0, if preparing
Solid medium then adds in the agar of 1.5 weight %.Here, the medium component content of culture yeasts bacterium can be in composite bacteria agent
It ignores, the removal efficiency of the aflatoxin of composite bacteria agent can't be had an impact.
Composite bacteria agent according to the present invention, also containing the medium component for being useful for culture Penicillium notatum.Wherein, Penicillium notatum is trained
Foster base can be the various conventional culture mediums in this field, such as can be Czapek's medium, and the composition of Czapek's medium includes：Nitre
Sour sodium 3-5g/L, dipotassium hydrogen phosphate 1-3g/L, magnesium sulfate (MgSO4·7H2O) 0.5-1g/L, potassium chloride 0.5-1g/L, sulfuric acid are sub-
Iron 0.01-0.5g/L, sucrose 30-40g/L, natural ph.Agar, which is added in, if solid medium is prepared causes fine jade in culture medium
Lipid concentration is 20g/L.Here, the medium component content that Penicillium notatum is cultivated in composite bacteria agent can be ignored, it can't
The removal efficiency of the aflatoxin of composite bacteria agent is had an impact.
The ratio of composite bacteria agent according to the present invention, saccharomycete and Penicillium notatum can be excellent in broad range
Selection of land, in terms of dry weight, the weight ratio of the saccharomycete and Penicillium notatum is 1：1-20, more preferably 1：5-20, further preferably
1：10-15, so as to improve the effect of aflatoxin degradation.
Composite bacteria agent according to the present invention can distinguish commercially available salt tolerance candidiasis and methamidophos
Routine culture is carried out, is then made dry powder, then by dry powder blend.Specifically, the preparation method of powder mixture can specifically wrap
It includes：By salt tolerance candidiasis and methamidophos respectively under 32-37 DEG C, 150-200rmp, in liquid YEB culture mediums
Cultivate 8-15h so that a concentration of 1-5 × 10 of salt tolerance candidiasis9CFU/ml, a concentration of 1-5 of methamidophos ×
109CFU/ml is then centrifuged for obtaining supernatant and sediment, and sediment is freeze-dried respectively to obtain thalline dry powder, then will
Obtained thalline dry powder blend, obtains powder mixture.Wherein, centrifugation and freeze-drying are this field conventional method, herein
It repeats no more.
The salt tolerance candidiasis (Candida versatilis) of the present invention can be that various commercially available salt tolerances are false
Silk saccharomycete (Candida versatilis), such as can be the salt tolerance Candida that preserving number is CGMCC No.3790
Bacterium.Lu Shi yeast (Zygosaccharomyces rouxii) can be various commercially available Lu Shi yeast
(Zygosaccharomyces rouxii), such as can be the Lu Shi yeast that preserving number is CGMCC No.2.371.
In a kind of preferred embodiment of the present invention, the saccharomycete is salt tolerance candidiasis (Candida
Versatilis) and the mixture of Lu Shi yeast (Zygosaccharomyces rouxii), the salt tolerance candidiasis
(Candida versatilis) and Lu Shi yeast (Zygosaccharomyces rouxii) weight ratio is 1：10-20, so as to
The effect of aflatoxin degradation can be improved.
Commercially available Penicillium griseofulvum and penicillium chrysogenum can be carried out conventional training respectively by composite bacteria agent according to the present invention
It supports, is then made dry powder, then by dry powder blend.Specifically, the preparation method of powder mixture can specifically include：By sallow blueness
Under 32-37 DEG C, 150-250rmp, 8-20h is cultivated in liquid Czapek's medium respectively for mould and penicillium chrysogenum so that sallow is green
Mould a concentration of 1-5 × 109CFU/ml, a concentration of 1-5 × 10 of penicillium chrysogenum9CFU/ml is then centrifuged for obtaining supernatant and precipitation
Object, sediment is freeze-dried respectively to obtain thalline dry powder, and the thalline dry powder blend that then will be obtained obtains powder mixture.
Wherein, centrifugation and freeze-drying are this field conventional method, and details are not described herein.
The Penicillium griseofulvum (Penicillium griseofulvum) of the present invention can be various commercially available Penicillium griseofulvums
(Penicillium griseofulvum), such as can be the Penicillium griseofulvum that preserving number is CGMCC No.3.8152.Production is yellow green
Mould (Penicillium Chrysogenum) can be various commercially available penicillium chrysogenums, such as can be preserving number be CGMCC
The penicillium chrysogenum of No.3.7797.
In a kind of preferred embodiment of the present invention, the Penicillium notatum is Penicillium griseofulvum (Penicillium
Griseofulvum) and the mixture of penicillium chrysogenum (Penicillium Chrysogenum), the Penicillium griseofulvum
(Penicillium griseofulvum) and penicillium chrysogenum (Penicillium Chrysogenum) weight ratio is 1：1-10,
So as to improve the effect of aflatoxin degradation.
The preparation method of the composite bacteria agent of the present invention can include：By saccharomycete and Penicillium notatum dry powder blend and at room temperature
It is sealed preservation." room temperature " in the present invention refers generally to 20-30 DEG C.
Second aspect, the present invention provides application of the above-mentioned composite bacteria agent in aflatoxin degradation.
The third aspect the present invention provides a kind of feed or additive, contains above-mentioned compound bacteria in the feed or additive
Agent.
According to the present invention, the composite bacteria agent contained in the feed or additive preferably exists in the form of dry powder.
In preferred embodiments, feed or additive are at least 0.001g/kg, at least 0.01g/kg, at least 0.1g/
Kg, at least 1g/kg, at least 5g/kg, at least 7.5g/kg, at least 10.0g/kg, at least 15.0g/kg, at least 20.0g/kg, extremely
The level of few 25.0g/kg contains composite bacteria agent of the present invention.
According to the present invention, also containing physiologically acceptable carrier in the feed and additive, wherein, the physiology
Upper acceptable carrier is selected from least one of following substance：Maltodextrin, lime stone (calcium carbonate), cyclodextrin, wheat,
Wheat bran or wheat component, rice or rice bran, sucrose, starch, Na2SO4, talcum powder and PVA and their mixture.
It will be apparent for a person skilled in the art that these additives can be added to aflatoxin-contaminated feeding
Expect in material to reduce endotoxin level present in the feed of animal consumption.
The Feed Material of the present invention can include：A) cereal, for example, granule cereal (such as wheat, barley, naked barley, oat with
And combination thereof) and/or big grain cereal such as maize or sorghum；B) by-product from cereal, for example, it is corn protein powder, dry
Vinasse and soluble matter (DDGS), wheat bran, sizing, wheat wheat-middlings, rice bran, rice husk, oat shell, palm kernel and citrus pulp；c)
Ensilage；D) protein derived from following source：Such as soybean, sunflower, peanut, lupin, pea, broad bean, cotton, card
Nola, fish meal, dry plasma albumen, meat and bone meal, potato protein, whey, copra, sesame；E) from plant and animal source
The oil ＆ fat of acquisition；F) minerals and vitamins.
It will be apparent to one skilled in the art that feed according to the present invention or additive can also include other groups
Divide such as stabilizer and/or incremental agent and/or enzyme.
Preferably, the method for preparing feed or additive according to the present invention includes mixing step, which includes
The composite bacteria agent is mixed, is optionally mixed together at least one physiologically acceptable carrier.
It is apparent to the skilled person that, as precautionary step, can add an additive in any Feed Material.
In the preferred embodiment of the present invention, when the feed of the feed or additive for cultivated animals or addition
During agent, the feed or additive are also containing bacillus licheniformis, bacillus subtilis, bifidobacterium bifidum, enterococcus faecalis, dung
Enterococcus, lactoenterococcus, lactobacillus acidophilus, Lactobacillus casei, German-style Lactobacillus lactate subspecies, lactobacillus plantarum, lactic acid sheet
Coccus, Pediococcus pentosaceus, candida utili, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis,
Streptococcus thermophilus, lactobacillus reuteri, animal bifidobacteria, aspergillus oryzae, bacillus lentus, bacillus pumilus, fiber two
At least one of sugared lactobacillus, lactobacillus fermenti and lactobacillus delbruockii subspecies bulgaricus.
In another preferred embodiment of the present invention, when the feed or additive are ensilage or ox feed
When, the feed or additive are also containing production propionibacterium acide-propionici (Propionibacterium acidipropionici), cloth
In family name's lactobacillus (Lactobacillus buchneri) and Lactobacillus paracasei (Lactobacillus paracasei) extremely
Few one kind.
In another preferred embodiment of the present invention, when the feed or additive be broiler chicken, growing-finishing pig or
When the feed or additive of aquiculture animal, the feed or additive also contain bacillus coagulans (Bacillus
coagulans)。
In another preferred embodiment of the present invention, when the feed or additive are broiler chicken, meat duck, pig or shrimp
When feed or additive, the feed or additive also contain Brevibacillus laterosporus (Brevibacillus
laterosporu)。
The composite bacteria agent of the present invention is easily stored and transports, and can be with being mixed by the material of aflatoxin contamination
Aflatoxin is effectively removed, can also be added in feed as additive when preparing all feeds, with prevention
The generation of aflatoxin, therefore, composite bacteria agent of the invention can be handled easily or be added in Feed Material, so as to
The content of aspergillus flavus in feed is reduced, is conducive to raise the health of animal.In the present invention, composite bacteria agent can individually add or
Person is added to together with carrier acceptable in the above physiological in feed.
Fourth aspect, the present invention provides a kind of method of aflatoxin degradation, this method includes：To pending sample
Middle addition composite bacteria agent or additive, carry out haptoreaction 1-96h at neutral salt solution, 25-37 DEG C, wherein, it is described compound
Microbial inoculum is above-mentioned composite bacteria agent or above-mentioned additive.
Preferably, the catalytic time be 2-72h, more preferably more preferably 2-48h, 2-24h, further preferably
2-10h。
According to the method for the present invention, the salinity of neutral salt solution can be 100-500mg/L, wherein, the salt can be with
For sodium chloride and/or potassium chloride.
The method of aflatoxin degradation according to the present invention, the pending sample can be not by aflatoxin dirt
The feed of dye, or by the feed of aflatoxin contamination.
In preferred embodiments, feed or additive are at least 0.001g/kg, at least 0.01g/kg, at least 0.1g/
Kg, at least 1g/kg, at least 5g/kg, at least 7.5g/kg, at least 10.0g/kg, at least 15.0g/kg, at least 20.0g/kg, extremely
The level of few 25.0g/kg contains composite bacteria agent of the present invention.Herein in terms of dry weight.
The method of aflatoxin degradation according to the present invention, those skilled in the art know that haptoreaction is in liquid
It is middle to carry out, therefore, haptoreaction can be caused to be carried out under the conditions of solid content is 20-40 weight %.
The present invention will be described in detail by way of examples below.
YEB culture mediums form：Yeast extract 1g/L, peptone 5g/L, beef extract 5g/L, sucrose 5g/L,
MgSO42mmol/L, pH value 7.0 add in the agar of 1.5 weight % if solid medium is prepared.
Czapek's medium forms：Sodium nitrate 3g/L, dipotassium hydrogen phosphate 1g/L, magnesium sulfate (MgSO4·7H2O) 0.5g/L,
Potassium chloride 0.5g/L, ferrous sulfate 0.01g/L, sucrose 30g/L, natural ph.Adding in agar if solid medium is prepared makes
It is 20g/L to obtain agar concentration in culture medium.
Preparation example
By commercially available salt tolerance candidiasis, (purchased from CGMCC, preserving number is：CGMCC No.3790) and Lu Shi yeast
(purchased from CGMCC, preserving number is bacterium：CGMCC No.2.371) liquid YEB culture mediums are seeded in 1% inoculum concentration respectively respectively
In, it is cultivated under the conditions of 150rmp, 37 DEG C, the time of culture makes salt tolerance candidiasis a concentration of 109CFU/ml, Lu Shi ferment
A concentration of the 10 of female bacterium9Then dry powder is respectively prepared in CFU/ml, remix.
By commercially available Penicillium griseofulvum, (purchased from CGMCC, preserving number is：CGMCC No.3.8152) and penicillium chrysogenum (be purchased from
CGMCC, preserving number are：CGMCC No.3.7797) it is seeded in respectively in liquid Czapek's medium with 1% inoculum concentration respectively, in
200rmp, it cultivates under the conditions of 37 DEG C, the time of culture makes Penicillium griseofulvum a concentration of 109CFU/ml, penicillium chrysogenum it is a concentration of
109Then dry powder is respectively prepared in CFU/ml, remix.
The saccharomycete of above-mentioned preparation and Penicillium notatum dry powder blend is uniform, be made composite bacteria agent, each composite bacteria agent it is specific
Composition is referring to the following table 1.
Table 1
Embodiment 1-8
For illustrating degradation of the composite bacteria agent provided by the invention to aflatoxin.
Proportioning according to each embodiment in table 1 carries out the preparation of composite bacteria agent, then adds respectively into prepared microbial inoculum
Enter 0.5ppm aflatoxin B1s, then add in water and cause solid content for 30 weight %, addition toxin is after 500mg/L NaCl
Concentration, 37 DEG C, pH value for reaction 2h under conditions of 7 after, the sample handled well is taken to detect sample according to national standard GBT 30955-2014
The concentration of aflatoxin in product, and calculate toxin removal efficiency according to equation below (1).
Toxin is initial in toxin removal efficiency=(sample toxin concentration after toxin initial concentration-processing in sample)/sample
The formula (1) of concentration × 100%.
Embodiment 9
For illustrating degradation of the composite bacteria agent provided by the invention to aflatoxin
Method according to embodiment 1 carries out the test of the degradation of aflatoxin, unlike, use physiological saline
Washing 3 times is carried out to each bacterial strain after culture, last isoconcentration is resuspended in physiological saline.The computational methods of toxin removal efficiency with
Embodiment 1-8 is identical, and the result is shown in tables 2.
Embodiment 10
For illustrating degradation of the composite bacteria agent provided by the invention to aflatoxin
Method according to embodiment 1 carries out the test of the degradation of aflatoxin, unlike, aflatoxin is not
Aflatoxin G 1 is used using aflatoxin B1.The computational methods of toxin removal efficiency are identical with embodiment 1-8, result
It is shown in Table 2.
Comparative example 1
This comparative example is used for degradation of the composite bacteria agent to aflatoxin for illustrating reference.
The test of aflatoxin degradation is carried out according to the method for embodiment 1, unlike, used compound bacteria
Agent is only made of salt tolerance candidiasis and methamidophos.It the results are shown in Table 2.
Comparative example 2
This comparative example is used for degradation of the composite bacteria agent to aflatoxin for illustrating reference.
The test of aflatoxin degradation is carried out according to the method for embodiment 1, unlike, used compound bacteria
Agent is made of Penicillium griseofulvum and penicillium chrysogenum.It the results are shown in Table 2.
Comparative example 3
This comparative example is used for degradation of the composite bacteria agent to aflatoxin for illustrating reference.
The test of aflatoxin degradation is carried out according to the method for embodiment 1, unlike, by salt tolerance vacation silk ferment
Female bacterium (Candida versatilis) replaces with commercially available Pichia pastoris (Pichia pastoris) yeast, the results are shown in Table 2.
Table 2
| Number | Aflatoxin removal efficiency (%) |
| Embodiment 1 | 90 |
| Embodiment 2 | 88 |
| Embodiment 3 | 88 |
| Embodiment 4 | 81 |
| Embodiment 5 | 82 |
| Embodiment 6 | 85 |
| Embodiment 7 | 84 |
| Embodiment 8 | 80 |
| Embodiment 9 | 90 |
| Embodiment 10 | 78 |
| Comparative example 1 | 65 |
| Comparative example 2 | 55 |
| Comparative example 3 | 75 |
Particular kind of saccharomycete and Penicillium notatum are combined use, Neng Gouyou it can be seen from the data of upper table 2
Effect ground aflatoxin degradation, the stronger aflatoxin B1 of the carcinogenicity that can especially degrade.Creatively will in the present invention
Saccharomycete and Penicillium notatum cooperation are used to prepare composite bacteria agent, which can easily handle or be added to Feed Material
In, so as to reduce the content of aspergillus flavus in feed, be conducive to raise the health of animal.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail, within the scope of the technical concept of the present invention, a variety of simple variants can be carried out to technical scheme of the present invention, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (10)
1. a kind of composite bacteria agent, which is characterized in that the composite bacteria agent includes saccharomycete and Penicillium notatum, and the saccharomycete is salt tolerance
Candidiasis (Candida versatilis) and/or methamidophos (Zygosaccharomyces rouxii), it is described
Penicillium notatum is Penicillium griseofulvum (Penicillium griseofulvum) and/or penicillium chrysogenum (Penicillium
Chrysogenum)。
2. composite bacteria agent according to claim 1, wherein, the weight ratio in terms of dry weight of the saccharomycete and Penicillium notatum is
1：1-20, preferably 1：5-20, more preferably 1：10-15.
3. composite bacteria agent according to claim 1, wherein, the saccharomycete is salt tolerance candidiasis (Candida
Versatilis) and the mixture of Lu Shi yeast (Zygosaccharomyces rouxii), the salt tolerance candidiasis
(Candida versatilis) and Lu Shi yeast (Zygosaccharomyces rouxii) weight ratio is 1：10-20.
4. composite bacteria agent according to claim 1, wherein, the Penicillium notatum is Penicillium griseofulvum (Penicillium
Griseofulvum) and the mixture of penicillium chrysogenum (Penicillium Chrysogenum), the Penicillium griseofulvum
(Penicillium griseofulvum) and penicillium chrysogenum (Penicillium Chrysogenum) weight ratio is 1：1-10.
5. application of the composite bacteria agent in claim 1-4 described in any one in aflatoxin degradation.
6. a kind of feed or additive, which is characterized in that contain any one institute in claim 1-4 in the feed or additive
The composite bacteria agent stated.
7. feed according to claim 6 or additive, wherein, the feed or additive for cultivated animals feed or
During additive, the feed or additive are also containing bacillus licheniformis, bacillus subtilis, bifidobacterium bifidum, excrement intestines ball
Bacterium, enterococcus faecium, lactoenterococcus, lactobacillus acidophilus, Lactobacillus casei, German-style Lactobacillus lactate subspecies, lactobacillus plantarum, breast
Sour piece coccus, Pediococcus pentosaceus, candida utili, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, youth bifid
Bacillus, streptococcus thermophilus, lactobacillus reuteri, animal bifidobacteria, aspergillus oryzae, bacillus lentus, bacillus pumilus, fibre
Tie up at least one of disaccharides lactobacillus, lactobacillus fermenti and lactobacillus delbruockii subspecies bulgaricus；
When the feed or additive are ensilage or ox feed, the feed or additive are also containing production propionibacterium acide-propionici
(Propionibacterium acidipropionici), lactobacillus buchneri (Lactobacillus buchneri) and pair are done
At least one of Lactobacillus paracasei (Lactobacillus paracasei).
8. feed according to claim 6 or additive, wherein, the feed or additive are broiler chicken, growing-finishing pig
Or aquiculture animal feed or additive when, the feed or additive also contain bacillus coagulans (Bacillus
coagulans)；
When the feed or additive are broiler chicken, meat duck, pig or the feed of shrimp or additive, the feed or additive also contain
Brevibacillus laterosporus (Brevibacillus laterosporu).
A kind of 9. method of aflatoxin degradation, which is characterized in that this method includes：Compound bacteria is added in into pending sample
Agent or additive carry out haptoreaction 1-96h at neutral salt solution, 25-37 DEG C, wherein, the composite bacteria agent will for right
Seek the additive described in any one in composite bacteria agent or the claim 5-8 in 1-4 described in any one.
10. according to the method described in claim 9, wherein, relative to the pending sample of 1kg, the composite bacteria agent or addition
The dosage of agent is at least 0.001g.
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| CN109744368A (en) * | 2019-03-22 | 2019-05-14 | 东莞市澹一生物科技有限公司 | Utilize the method and feedstuff of wheat vinasse production feedstuff |
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| CN111575213B (en) * | 2020-05-29 | 2021-11-02 | 江南大学 | Microbial compound bacterium agent and application thereof in preparation of fermented feed |
| CN111961623A (en) * | 2020-08-25 | 2020-11-20 | 青岛农业大学 | Compound lactobacillus preparation and application thereof, compound mildew removing agent and application thereof |
| CN113403220A (en) * | 2021-05-24 | 2021-09-17 | 河北农业大学 | Composite microbial inoculant for lamb complete feed fermentation |
| CN114437983A (en) * | 2022-02-18 | 2022-05-06 | 广西优比特生物科技有限公司 | Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent and preparation method and application of microbial agent |
| CN114437983B (en) * | 2022-02-18 | 2023-07-21 | 广西优比特生物科技有限公司 | Enterococcus faecium GXSCU1 capable of efficiently degrading vomitoxin, microbial agent, and preparation method and application thereof |
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