CN108239156A - A kind of method for preparing function converted starch of being degraded using enzyme - Google Patents
A kind of method for preparing function converted starch of being degraded using enzyme Download PDFInfo
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- CN108239156A CN108239156A CN201611201748.3A CN201611201748A CN108239156A CN 108239156 A CN108239156 A CN 108239156A CN 201611201748 A CN201611201748 A CN 201611201748A CN 108239156 A CN108239156 A CN 108239156A
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- starch
- enzyme
- converted starch
- function converted
- degraded
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/04—Extraction or purification
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B30/00—Preparation of starch, degraded or non-chemically modified starch, amylose, or amylopectin
- C08B30/12—Degraded, destructured or non-chemically modified starch, e.g. mechanically, enzymatically or by irradiation; Bleaching of starch
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a kind of methods degraded using enzyme and prepare function converted starch.The present invention is using cork oak starch as raw material, and during converted starch is prepared, addition carbohydrase is digested, and overcomes the shortcomings that other preparation methods are of high cost, preparation process is slow.
Description
Technical field
The present invention relates to a kind of converted starch, more particularly to a kind of method for preparing function converted starch of being degraded using enzyme,
Belong to starch manufacture field.
Background technology
Nearly ten years, the yield of world's converted starch increases rapidly, and the annual output of world's converted starch is close to 800 at present
More ten thousand tons, wherein more than 300 ten thousand tons of the U.S., more than 200 ten thousand tons of Europe, more than 60 ten thousand tons of Japan, more than 50 ten thousand tons of Thailand, China more than 100 ten thousand
Ton.The application of China's converted starch still falls within emerging service, no matter from the type, quality or application range of converted starch, all
There is larger difference with foreign countries.
At present, it prepares converted starch and is mainly the following method：First, physical method, for example, by ultrasonic irradiation or
Person's spray art；Second is that using mechanical means, such as pass through the mechanical impacts such as ball-milling technology；Third is biochemical method, such as alcohol
Denaturation or sour water solution, in above-mentioned several method, the production cost of the mechanical impacts method such as ultrasonic irradiation, ball-milling technology compared with
Height is not easy to realize industrialization；And spray-on process and alcohol are denaturalized that be formed is a kind of solid popcorn polymer sphere, suction-operated only occurs
In the gully of surface irregularity, adsorbance is limited, and application prospect is pessimistic.Acid-hydrolysis method reaction rate under gelatinization point
Relatively slow, degradation differs, and randomness is strong, is not easy to be formed poroid, limits the application of acid system.
Enzyme degradation technique is catalyzed using enzyme, can efficiently be reacted, hydrolysising by-product is few.Cork oak seed
Starch-containing 30-50%, the present invention prepare a kind of high adsorption using cork oak starch as raw material using enzyme degradation technique method
The function converted starch of energy, the converted starch of preparation can be as the carrier of adsorption function substance.
Invention content
The object of the present invention is to provide one kind using cork oak starch as raw material, degraded using enzyme and prepare function converted starch
Method, main method and step is as follows：
(1) suitable cork oak starch is first taken to add in a certain amount of distilled water, heating is gelatinized；
(2) the starch solution of gelatinization is cooled to room temperature, then adds in lauryl sodium sulfate, and constantly stirred to dissolve
It is even；
(3) again by system heating water bath, and a certain amount of carbohydrase is added in, and constantly stirred to dissolve uniformly；
(4) a certain amount of succinic acid diepoxy propyl alcohol ester solution is added in, is fully reacted；
(5) pour the mixture into acetone soln, filter again；
(6) filtration product is poured into clear water again, filtered again；
(7) filtration product is collected, vacuum drying is placed, up to a kind of function converted starch after drying.
Preferentially, step (1) in, heating temperature be 40-50 DEG C.
Preferentially, step (2) in, the amount of the lauryl sodium sulfate of addition and the ratio of starch are 1mL:5g.
Preferentially, step (3) in, the carbohydrase of addition and the quality of starch are 1 than ratio：100, heating temperature 40-70
DEG C, reaction time 5-10h.
Preferentially, step (4) in, the succinic acid diepoxy propyl alcohol ester of addition and the ratio of starch are 1mL:100g.
Above-mentioned starch used is produced as follows：
(1) cork oak kernel is taken to be impregnated in water；
(2) the kernel of immersion is shelled with crusher；
(3) the kernel after shelling is finely ground into slurry, then by screen centrifuge, starch magma is made to be detached with kernel slag；
(4) the starch magma separated enters desander, removes the impurity such as sandstone in slurries；
(5) the slurries after desanding are refined by cyclone, remove the impurity such as isolating protein, fine fibre；
(6) washing starch for several times, finally dry to get a kind of cork oak starch by filtering.
The present invention has following advantages and characteristic：
(1) the converted starch stability prepared is good；
(2) preparation process is simple, easily realizes industrialization.
Specific embodiment
Embodiment one：
5g cork oak starch is taken, 100ml distilled water is added in, is placed in 40 DEG C of environment and carries out heating water bath, and continuous with blender
Stirring, is allowed to form homogeneous paste starch solution, and 1mL dodecyl sulphates are added in after paste starch solution is cooled to room temperature
Sodium, and stir to dissolve uniformly, system is heated, when temperature reaches 40 DEG C, adds 0.05g carbohydrase, and stirring makes to fill
Point, after reacting 5h, the succinic acid diepoxy propyl alcohol ester of 0.05g is added, it is then clear with acetone after stirring makes it fully react
It washes, filter, be washed with water, filter, filtration product is put into vacuum drying to get a kind of function converted starch.
Embodiment two：
10g cork oak starch is taken, 200ml distilled water is added in, is placed in 45 DEG C of environment and carries out heating water bath, and continuous with blender
Stirring, is allowed to form homogeneous paste starch solution, and 2mL dodecyl sulphates are added in after paste starch solution is cooled to room temperature
Sodium, and stir to dissolve uniformly, system is heated, when temperature reaches 60 DEG C, adds 0.1g carbohydrase, and stirring makes to fill
Point, after reacting 8h, the succinic acid diepoxy propyl alcohol ester of 0.1g is added, after stirring makes it fully react, then cleaned with acetone,
Filtering, is washed with water, and filters, and filtration product is put into vacuum drying to get a kind of function converted starch.
Embodiment three：
40g cork oak starch is taken, 600ml distilled water is added in, is placed in 50 DEG C of environment and carries out heating water bath, and continuous with blender
Stirring, is allowed to form homogeneous paste starch solution, and 8mL dodecyl sulphates are added in after paste starch solution is cooled to room temperature
Sodium, and stir to dissolve uniformly, system is heated, when temperature reaches 70 DEG C, adds 0.4g carbohydrase, and stirring makes to fill
Point, after reacting 10h, the succinic acid diepoxy propyl alcohol ester of 0.4g is added, it is then clear with acetone after stirring makes it fully react
It washes, filter, be washed with water, filter, filtration product is put into vacuum drying to get a kind of function converted starch.
Claims (6)
1. it is a kind of using enzyme degrade prepare function converted starch method, it is characterised in that the preparation method as follows into
Row：
(1) suitable cork oak starch is first taken to add in a certain amount of distilled water, heating is gelatinized；
(2) the starch solution of gelatinization is cooled to room temperature, then adds in lauryl sodium sulfate, and constantly stirred to dissolve
It is even；
(3) again by system heating water bath, and a certain amount of carbohydrase is added in, and constantly stirred to dissolve uniformly；
(4) a certain amount of succinic acid diepoxy propyl alcohol ester solution is added in, is fully reacted；
(5) pour the mixture into acetone soln, filter again；
(6) filtration product is poured into clear water again, filtered again；
(7) filtration product is collected, vacuum drying is placed, up to a kind of function converted starch after drying.
2. a kind of method for preparing function converted starch of being degraded using enzyme as described in claim 1, which is characterized in that step is (1)
In, heating temperature is 40-50 DEG C.
3. a kind of method for preparing function converted starch of being degraded using enzyme as described in claim 1, which is characterized in that step is (2)
In, the amount of the lauryl sodium sulfate of addition and the ratio of starch are 1mL:5g.
4. a kind of method for preparing function converted starch of being degraded using enzyme as described in claim 1, which is characterized in that step is (3)
In, the carbohydrase of addition and the quality of starch are 1 than ratio：100, heating temperature is 40-70 DEG C, reaction time 5-10h.
5. a kind of method for preparing function converted starch of being degraded using enzyme as described in claim 1, which is characterized in that step is (4)
In, the succinic acid diepoxy propyl alcohol ester of addition and the ratio of starch are 1mL:100g.
6. such as a kind of method for preparing function converted starch using enzyme degradation of claim 1-5 any one of them, feature exists
In above-mentioned starch used is produced as follows：
(1) cork oak kernel is taken to be impregnated in water；
(2) the kernel of immersion is shelled with crusher；
(3) the kernel after shelling is finely ground into slurry, then by screen centrifuge, starch magma is made to be detached with kernel slag；
(4) the starch magma separated enters desander, removes the impurity such as sandstone in slurries；
(5) the slurries after desanding are refined by cyclone, remove the impurity such as isolating protein, fine fibre；
(6) washing starch for several times, finally dry to get a kind of cork oak starch by filtering.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611201748.3A CN108239156A (en) | 2016-12-23 | 2016-12-23 | A kind of method for preparing function converted starch of being degraded using enzyme |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611201748.3A CN108239156A (en) | 2016-12-23 | 2016-12-23 | A kind of method for preparing function converted starch of being degraded using enzyme |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108239156A true CN108239156A (en) | 2018-07-03 |
Family
ID=62703276
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611201748.3A Withdrawn CN108239156A (en) | 2016-12-23 | 2016-12-23 | A kind of method for preparing function converted starch of being degraded using enzyme |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108239156A (en) |
-
2016
- 2016-12-23 CN CN201611201748.3A patent/CN108239156A/en not_active Withdrawn
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Application publication date: 20180703 |
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