CN108239143A - A kind of anti-trichothecium roseum peptide and its preparation method and application - Google Patents
A kind of anti-trichothecium roseum peptide and its preparation method and application Download PDFInfo
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- CN108239143A CN108239143A CN201810051424.9A CN201810051424A CN108239143A CN 108239143 A CN108239143 A CN 108239143A CN 201810051424 A CN201810051424 A CN 201810051424A CN 108239143 A CN108239143 A CN 108239143A
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- peptide
- gly
- trichothecium roseum
- ser
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
Abstract
The present invention relates to a kind of anti-trichothecium roseum peptide, the amino acid sequence of the peptide is Gly Gly Ser Gly Gly Gly Ser Ser Gly Gly Ser Ile Gly Gly Arg.Meanwhile the invention also discloses the preparation method and applications of the anti-trichothecium roseum peptide.The present invention shows there is that stability is good, antibacterial activity is high through antibacterial tests, stability test.
Description
Technical field
The present invention relates to a kind of polypeptide more particularly to a kind of anti-trichothecium roseums（Trichothecium roseum）Peptide and
Preparation method and application.
Background technology
Trichothecium roseum（T. roseum）It is a kind of disease fungus that can not be ignored in postharvest disease of fruits and vegetables, the cause of disease
Fungi can be entered by wound in product body during post harvest transport, and then be caused rotten.It is reported that developed country have 15% ~
25% fresh fruit of vegetables, which is lost in after adopting, to rot, and in developing country, refrigerating equipment is stored and transported due to lacking, fruits and vegetables rot to damage
Mistake rate is up to 20% ~ 50%.Generally use chemical bactericide effectively to control disease, but there are chemical bactericide residual, environment are dirty
The problems such as dye and pathogen develop immunity to drugs.Therefore, there is an urgent need for find new, safer effective method control adopt after disease
Evil.
Antibacterial peptide is a kind of biologically active small-molecular peptides, is the important component of biological defensive system.Antibacterial
Peptide derives from a wealth of sources, the antibacterial found from insect, mammal, amphibian, plant, bacterium, marine organisms etc. at present
About 1598 kinds of peptide.These antibacterial peptides have broad-spectrum antibacterial action, and to normal eukaryocyte, mammalian cell without
Toxicity or hypotoxicity, therefore, antibacterial peptide are expected to as the antibacterials of a new generation, and are raised in cosmetics, biological pesticide, animal
Feed additives, antiseptics for natural food, animal and plant disease resisting genetic engineering etc. have broad application prospects.
Antibacterial peptide is formed according to its amino acid and structure feature can be divided into：(1) there is α-helixstructure antibacterial peptide；(2) have
There is beta sheet structure antibacterial peptide；(3) the polypeptide of cysteine is rich in；Rich in histidine, arginine, proline, glycine it is more
Peptide；(5) the polypeptide being made of rare recombination amino acid.Wherein, the antibacterial peptide rich in glycine has Low haemolysis activity, may be used also
To inhibit Gram-negative bacteria, positive bacteria and the growth of fungi.For example, the C-terminal of dipteron peptide (Diptericins) is rich in sweet ammonia
Sour residue can inhibit Gram-negative bacteria growth；There is scholar from bacillus amyloliquefaciens（Bacillus amyloliquefaciens）The p277 D1 rich in glycine is isolated, it is found that the antibacterial peptide in minimal inhibitory concentration is 8 ~ 64 μ g/
It can inhibit Gram-positive and negative bacterium during mL.But since antibacterial peptide is with the limitation of the factors such as low stability, production cost height
Being widely used for antibacterial peptide, therefore researcher is dedicated to novel antimicrobial peptide of the research and development with high stability hypotoxicity.
Invention content
The technical problems to be solved by the invention are to provide the anti-trichothecium roseum that a kind of stability is good, antibacterial activity is high
Peptide.
Another technical problem to be solved by this invention is to provide the preparation method of the anti-trichothecium roseum peptide.
Third technical problem to be solved by this invention is to provide the application of the anti-trichothecium roseum peptide.
To solve the above problems, a kind of anti-trichothecium roseum peptide of the present invention, it is characterised in that：The amino acid of the peptide
Sequence is Gly- Gly- Ser- Gly- Gly- Gly- Ser- Ser- Gly- Gly- Ser- Ile- Gly- Gly-
Arg。
A kind of preparation method of anti-trichothecium roseum peptide as described above, includes the following steps：
(1) picking bacillus pumilus（B. pumilus）1 ~ 2 ring of bacterial strain pure culture is inoculated on LB fluid nutrient mediums, in 37
DEG C, under the conditions of 180 r/min after 2 ~ 3d of shaken cultivation, obtain zymotic fluid, which is centrifuged with the rate of 10000r/min
20min obtains supernatant；
The bacillus pumilus（B. pumilus）It is in the deposit number of China General Microbiological culture presevation administrative center
CGMCC NO.15031（Depositary institution address：Chinese Beijing Institute of Microorganism, Academia Sinica；Preservation date：2017
December 07）；
(2) by the supernatant successively through macroporous adsorption resin chromatography, size exclusion chromatography and the reversed high pressure liquid of preparative
Analysis, obtains eluent；
(3) the eluent is in the usual way after being concentrated by ultrafiltration, drying, be lyophilized to get the anti-trichothecium roseum peptide of purifying.
(2) the condition of middle macroporous adsorption resin chromatography refers to use AB-8 macroporous absorbent resins the step, with 2 BV/h's
Speed loading, and volumetric concentration is used to be eluted for 60 ~ 70% ethanol solution.
The condition of the step (2) middle size exclusion chromatography refers to using sephadex g-100 as filler, and uses
The phosphate buffer solution of a concentration of 15 ~ 25mmol/L and pH=6 ~ 7 elutes.
(2) the condition of the reversed high performance liquid chroma- tography of middle preparative refers to use C18 columns the step, using sample concentration as 5
Mg/ml, applied sample amount are 200 μ L, elution flow rate 1ml/min, the condition that column temperature is 25 DEG C carry out linear elution；Eluting A phases is
Containing 0.1 % TFA（Trifluoroacetic acid）Aqueous solution, B phases are containing 0.1% TFA（Trifluoroacetic acid）Acetonitrile solution；Elution program is：A phases：
10 % ~ 5 %, 0 ~ 5 min；5 % ~ 0 %, 5 ~ 10 min；0 % ~ 10 %, 10 ~ 11 min；10 %, 11 ~ 30min.
A kind of application of anti-trichothecium roseum peptide as described above, it is characterised in that：The anti-trichothecium roseum peptide, which is used as, to be had
All kinds of pharmaceutical formulations are made with pharmaceutically acceptable any carrier according to a conventional method in effect ingredient.
Compared with the prior art, the present invention has the following advantages：
1st, the present invention is by by macroporous adsorption resin chromatography, size exclusion chromatography and preparative three kinds of layers of reversed high performance liquid chroma- tography
The tomographic system of composition is analysed, removing respectively in zymotic fluid with the anti-trichothecium roseum peptide of purpose has charge differences, polarity difference, big
Other substances such as foreign protein of small difference, so as to achieve the effect that purifying.
2nd, the anti-trichothecium roseum peptide after the present invention is purified is by combining LC-MS/MS, so as to obtain antibacterial peptide
Amino acid sequence.
Pink list is resisted to what is be prepared using Q Exactive mass spectrometer (Thermo fisher)
End spore peptide carries out analysis detection, wherein：Chromatographic column is 300 μm of i.d. x 5 mm, packed with Acclaim
PepMap RPLC C18, 5 μm, 100 Å, nanoViper （Purchased from Thermo companies）；Mobile phase A：0.1% formic acid, 2%
ACN；Mobile phase B：0.1% formic acid, 80% ACN；Column temperature：25 DEG C, Detection wavelength：280nm；Maximum pressure：200bar；Time：
78min；Flow velocity：300 nL/min；Analysis gradient is shown in Table 1, and testing result is shown in Fig. 1.
1 LC of table analyzes gradient
A) first mass spectrometric parameter： Resolution：70,000; AGC target：3e6;
Maximum IT：40 ms; Scan range：350 to 1800 m/z.
B) second order ms parameter： Resolution：17,500; AGC target：1e5; Maximum IT： 60 ms;
TopN ：20; NCE / stepped NCE：27
Testing result is shown in Fig. 2.
The result shows that：The amino acid sequence of the anti-trichothecium roseum peptide of the present invention is respectively： Gly- Gly- Ser- Gly-
Gly- Gly- Ser- Ser- Gly- Gly- Ser- Ile- Gly- Gly- Arg。
3rd, the property difference of the invention by using the characteristics of anti-trichothecium roseum peptide between different chromatography methods, not only
Scientifically and rationally confrontation trichothecium roseum peptide is isolated and purified, and is also carried for the detection of the amino acid sequence of anti-trichothecium roseum peptide
Foundation is supplied.
4th, the present invention shows there is that stability is good, antibacterial activity is high through antibacterial tests, stability test.
【Reference examples】With document（Various concentration sodium metasilicate and corresponding pH inhibit trichothecium roseum effect under the conditions of in vivo and in vitro
Fruit is compared）In sodium metasilicate be control, sodium silicate silicate is processed into 20,40,60,80,100mmol/L, observe it to powder
The influence of clearance permit end spore mycelia growth.
The result shows that：When sodium silicate silicate reaches 20mmol/L, inhibition starts to show, and acts on apparent.
【Antibacterial tests】
Antibacterial tests are carried out to the anti-trichothecium roseum peptide of the present invention using standard agar hole diffusion method respectively.The silicic acid of reference examples
Sodium uses tablet face-off method, and PDA culture medium after sterilizing is cooled to temperature 60 C, the sodium metasilicate of different quality is separately added into, makes
Sodium metasilicate final concentration of 20,40,60,80,100 mmol/L, fully dissolving after, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, then by the powder of a diameter of 5mm
Clearance permit end spore bacteria cake places tablet center, 28 DEG C of culture 5d.
The sodium metasilicate of the anti-trichothecium roseum peptide of the present invention and reference examples is configured to the solution of same concentrations respectively, with pink
Single-ended spore（T. roseum）For experimental strain, experimental strain spore suspension is placed on tablet center, with sterilized punching
Device（Diameter 5mm）Away from being punched at spore suspension 2cm, each antibacterial peptide and sodium silicate solution are added dropwise respectively in hole, in 28 DEG C of trainings
5d is supported, inhibition zone size is observed, the results are shown in Table 2.
The antibacterial activity of 2 each antibacterial peptide of table
The result shows that：When anti-trichothecium roseum peptide concentration is 1 μ g/mL, inhibit trichothecium roseum（T. roseum）Effect
Start to show, and act on apparent.
【Stability test】
The anti-trichothecium roseum peptide of the present invention at 4 DEG C is preserved 30 days, then according to antibacterial tests are carried out, the results are shown in Table 3.
The stability of 3 each antibacterial peptide of table
The result shows that：The anti-trichothecium roseum peptide has strong resistance to acid and alkali and heat-resisting（Less than 80 DEG C）Stability.It is in addition, anti-pink
Single-ended spore peptide has papain good tolerance, weaker to pepsin, trypsin resistance.
Description of the drawings
The specific embodiment of the present invention is described in further detail below in conjunction with the accompanying drawings.
Fig. 1 is the preparative RP-HPLC figures of the anti-trichothecium roseum peptide of the present invention.
Fig. 2 is the MALDI-TOF/MS figures of the anti-trichothecium roseum peptide of the present invention.
Specific embodiment
A kind of anti-trichothecium roseum peptide, the amino acid sequence of the peptide is Gly- Gly- Ser- Gly- Gly- Gly-
Ser- Ser- Gly- Gly- Ser- Ile- Gly- Gly- Arg。
The preparation method of the anti-trichothecium roseum peptide, includes the following steps：
(1) picking bacillus pumilus（B. pumilus；Bacillus pumilus is in China General Microbiological culture presevation management
The deposit number of the heart be CGMCC NO.15031, depositary institution address：Chinese Beijing Institute of Microorganism, Academia Sinica；It protects
Hide the date：On December 07th, 2017）1 ~ 2 ring of bacterial strain pure culture is inoculated on LB fluid nutrient mediums, in 37 DEG C, 180 r/min
Under the conditions of after 2 ~ 3d of shaken cultivation, obtain zymotic fluid, which centrifuges 20min with the rate of 10000r/min, obtains supernatant
Liquid.
(2) by supernatant successively through macroporous adsorption resin chromatography, size exclusion chromatography and the reversed high pressure liquid of preparative
Analysis, obtains eluent.Detailed process is as follows：
For AB-8 macroporous absorbent resins after absolute ethyl alcohol balances, supernatant uses volumetric concentration with the speed loading of 2 BV/h
Ethanol solution for 60 ~ 70% is eluted（Different concentration of alcohol can also be used to carry out gradient elution as eluent）, often
2min collects an eluent and carries out bacteriostatic test using standard agar hole diffusion method, collects the eluent with inhibition zone
A。
Size exclusion chromatography is first put down using sephadex g-100 as filler using the phosphate buffer solution of pH 6.8
Weighing apparatus, then eluent A is eluted using the phosphate buffer solution of a concentration of 15 ~ 25mmol/L and pH=6 ~ 7, it is received per 4min
Eluent of collection simultaneously carries out bacteriostatic test, collects the eluent B with inhibition zone.
Using SunFireTMPrep C18 columns（10 μm, 10x150mm, Waters, US）Eluent B is made
The reversed high performance liquid chroma- tography of standby type.Sample concentration is 5 mg/ml, applied sample amount is 200 μ L, elution flow rate 1ml/min, column
Temperature carries out linear elution under conditions of being 25 DEG C.It is containing 0.1 % TFA to elute A phases（Trifluoroacetic acid）Aqueous solution, B phases are containing 0.1%
TFA（Trifluoroacetic acid）Acetonitrile solution；Elution program is：A phases：10 % ~ 5 %, 0 ~ 5 min；5 % ~ 0 %, 5 ~ 10 min；0 %~10
%, 10 ~ 11 min；10 %, 11 ~ 30min.Eluent is collected after elution.
(3) eluent is in the usual way after being concentrated by ultrafiltration, drying, be lyophilized to get the anti-trichothecium roseum peptide of purifying.
In addition, the anti-trichothecium roseum peptide of the present invention can be obtained by chemical synthesis, it can also be according to the amino acid of the peptide
Sequence construct obtains above-mentioned anti-trichothecium roseum peptide expression vector, and then obtains containing above-mentioned anti-trichothecium roseum peptide expression vector
Bacterial strain and ferment to it.
The application of the anti-trichothecium roseum peptide refers to：The anti-trichothecium roseum peptide as active ingredient according to a conventional method with medicine
All kinds of pharmaceutical formulations are made in acceptable any carrier on.
It should be understood that embodiment discussed here and embodiment simply to illustrate that, person skilled in the art can be proposed
Various modifications and variations, such modifications and variations are included within spirit herein spirit and scope and appended claim
In the range of.
Claims (6)
1. a kind of anti-trichothecium roseum peptide, it is characterised in that：The amino acid sequence of the peptide is Gly- Gly- Ser- Gly-
Gly- Gly- Ser- Ser- Gly- Gly- Ser- Ile- Gly- Gly- Arg。
2. a kind of preparation method of anti-trichothecium roseum peptide as described in claim 1, includes the following steps：
(1) picking bacillus pumilus（B. pumilus）1 ~ 2 ring of bacterial strain pure culture is inoculated on LB fluid nutrient mediums, in 37
DEG C, under the conditions of 180 r/min after 2 ~ 3d of shaken cultivation, obtain zymotic fluid, which is centrifuged with the rate of 10000r/min
20min obtains supernatant；
The bacillus pumilus（B. pumilus ）It is in the deposit number of China General Microbiological culture presevation administrative center
CGMCC NO.15031；
(2) by the supernatant successively through macroporous adsorption resin chromatography, size exclusion chromatography and the reversed high pressure liquid of preparative
Analysis, obtains eluent；
(3) the eluent is in the usual way after being concentrated by ultrafiltration, drying, be lyophilized to get the anti-trichothecium roseum peptide of purifying.
3. a kind of preparation method of anti-trichothecium roseum peptide as claimed in claim 2, it is characterised in that：The step (2) in it is big
The condition of macroporous adsorbent resin chromatography refers to using AB-8 macroporous absorbent resins, with the speed loading of 2 BV/h, and it is dense using volume
Spend the ethanol solution elution for 60 ~ 70%.
4. a kind of preparation method of anti-trichothecium roseum peptide as claimed in claim 2, it is characterised in that：The step (2) middle ruler
The condition of very little exclusion chromatography refers to using sephadex g-100 as filler, and uses a concentration of 15 ~ 25mmol/L and pH=6
~ 7 phosphate buffer solution elution.
5. a kind of preparation method of anti-trichothecium roseum peptide as claimed in claim 2, it is characterised in that：The step (2) middle system
The condition of the standby reversed high performance liquid chroma- tography of type refers to using C18 columns, is 200 μ L by 5 mg/ml, applied sample amount of sample concentration, washes
The condition that separation of flow speed is 1ml/min, column temperature is 25 DEG C carries out linear elution；It is containing 0.1 % TFA aqueous solutions to elute A phases, and B phases are
Containing 0.1% TFA acetonitrile solutions；Elution program is：A phases：10 % ~ 5 %, 0 ~ 5 min；5 % ~ 0 %, 5 ~ 10 min；The % of 0 % ~ 10,
10~11 min；10 %, 11 ~ 30min.
6. a kind of application of anti-trichothecium roseum peptide as described in claim 1, it is characterised in that：The anti-trichothecium roseum peptide is made
All kinds of pharmaceutical formulations are made with pharmaceutically acceptable any carrier according to a conventional method for active ingredient.
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| CN201810051424.9A CN108239143A (en) | 2018-01-19 | 2018-01-19 | A kind of anti-trichothecium roseum peptide and its preparation method and application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810051424.9A CN108239143A (en) | 2018-01-19 | 2018-01-19 | A kind of anti-trichothecium roseum peptide and its preparation method and application |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002030975A2 (en) * | 2000-10-11 | 2002-04-18 | Xoma Technology, Ltd. | Antifungal peptides |
| CN103266155A (en) * | 2013-05-21 | 2013-08-28 | 湖南农业大学 | Method for separating and purifying antibacterial peptide of endophytic bacteria strain though high efficiency liquid chromatography |
| CN104212857A (en) * | 2014-04-02 | 2014-12-17 | 曹庸 | Method for separation purification of antibacterial peptide from lactobacillus paracasei fermented milk as raw material |
-
2018
- 2018-01-19 CN CN201810051424.9A patent/CN108239143A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002030975A2 (en) * | 2000-10-11 | 2002-04-18 | Xoma Technology, Ltd. | Antifungal peptides |
| CN103266155A (en) * | 2013-05-21 | 2013-08-28 | 湖南农业大学 | Method for separating and purifying antibacterial peptide of endophytic bacteria strain though high efficiency liquid chromatography |
| CN104212857A (en) * | 2014-04-02 | 2014-12-17 | 曹庸 | Method for separation purification of antibacterial peptide from lactobacillus paracasei fermented milk as raw material |
Non-Patent Citations (1)
| Title |
|---|
| HAIJIAO YAN ET AL.: "Two novel cationic antifungal peptides isolated from Bacillus pumilus HN-10 and their inhibitory activity against Trichothecium roseum", 《WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY》 * |
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Application publication date: 20180703 |