CN108235732B - Raman detection auxiliary device, Raman detection equipment and method - Google Patents
Raman detection auxiliary device, Raman detection equipment and method Download PDFInfo
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- CN108235732B CN108235732B CN201780002552.8A CN201780002552A CN108235732B CN 108235732 B CN108235732 B CN 108235732B CN 201780002552 A CN201780002552 A CN 201780002552A CN 108235732 B CN108235732 B CN 108235732B
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
Abstract
The disclosure provides a Raman detection auxiliary device, Raman detection equipment and a Raman detection method, belongs to the technical field of spectrum detection, and can at least partially solve the problem that an existing Raman chip can only complete single-chip measurement once and is poor in consistency among multiple measurements. The raman detection auxiliary device of the present disclosure includes: an insertion port for inserting a plurality of Raman chips; and the conveying mechanism is used for sequentially conveying the plurality of inserted Raman chips to a preset position so that the Raman detection equipment can detect the Raman chips. The Raman detection auxiliary device can realize batch measurement of Raman chips, and improves detection efficiency.
Description
Technical Field
The disclosure belongs to the technical field of spectrum detection, and particularly relates to a Raman detection auxiliary device, Raman detection equipment and Raman detection method.
Background
The current Raman detection equipment is to coat liquid to be detected on a Raman chip, and put the Raman chip at the front end of a micro-Raman spectrometer or a common Raman spectrometer for measurement after natural drying. Because the position of the raman chip for each measurement is not fixed, the adjustment of the focal length and the transverse position of the spectrometer is required before each measurement, the time for each adjustment exceeds the time for measurement, and a sufficient signal-to-noise ratio is difficult to achieve, and the measurement mode may cause a series of problems as follows: the single measurement consumes long time, cannot be operated in batch, and only can be finished by one-chip measurement; the signal-to-noise ratio is difficult to reach the optimum, and the noise level is high; the deviation caused by position errors among multiple measurements is large, and the consistency is poor; inconvenient measurement, is only suitable for laboratory operation, and can not be suitable for field operation.
Therefore, it is an urgent technical problem to design a raman detection auxiliary device, a raman detection apparatus and a method which are more convenient to operate.
Disclosure of Invention
The present disclosure is directed to at least partially solve the above-mentioned problems, and provides a raman detection assisting device, a raman detection apparatus, and a raman detection method. Compared with the existing Raman detection equipment, the Raman detection auxiliary device, the Raman detection equipment and the Raman detection method improve the Raman detection efficiency.
According to an aspect of the present disclosure, there is provided a raman detection assistance device including: an insertion port for inserting a plurality of Raman chips; and the conveying mechanism is used for sequentially conveying the plurality of inserted Raman chips to a preset position so that the Raman detection equipment can detect the Raman chips.
According to an embodiment of the present disclosure, the raman detection assisting apparatus further includes: a chip placing member for placing the plurality of raman chips in order, the raman detection assisting device being inserted through the insertion port; and the mounting part is used for fixing the chip placing part.
According to one embodiment of the present disclosure, the chip placement member includes a tape or a chain.
According to one embodiment of the present disclosure, the transfer mechanism includes: and the ejection button is used for ejecting the Raman chip from the preset position.
According to one embodiment of the present disclosure, the transfer mechanism includes: and the stepping motor is used for sequentially conveying the plurality of Raman chips to the preset position.
According to an embodiment of the present disclosure, the raman detection assisting apparatus further includes: and the accessory metal contact is arranged on the contact surface of the Raman detection auxiliary device and is used for data communication and/or power supply between the Raman detection auxiliary device and the Raman detection equipment.
According to one embodiment of the disclosure, the raman chip is a raman enhancement chip with a predetermined size, the raman enhancement chip includes a nano enhancement effective region with a preset area, and the nano enhancement effective region is used for placing a sample to be detected.
According to an embodiment of the present disclosure, the raman detection assisting apparatus further includes: and the chip information reading device is used for reading the chip information of the Raman chip.
According to one embodiment of the present disclosure, the chip information reading device includes a magnetic stripe reading device and/or a two-dimensional code reading device.
According to another aspect of the present disclosure, there is provided a raman detection apparatus comprising: an apparatus main body; the raman detection assistance device according to any one of the above embodiments.
According to one embodiment of the disclosure, when the raman detection device is in an operating state, the laser focus of the raman detection device is focused on the nano-enhanced active area of the raman chip at the predetermined position.
According to one embodiment of the present disclosure, the nanoenhanced active region of the raman chip is a right central region of the raman chip.
According to an embodiment of the present disclosure, the raman detection apparatus further includes: the equipment metal contact is arranged on the contact surface of the equipment main body; wherein the accessory metal contact of the Raman auxiliary detection device and the equipment metal contact are correspondingly arranged and used for data communication and/or power supply between the Raman auxiliary detection device and the equipment main body.
According to still another aspect of the present disclosure, there is provided a raman detection method applied to a raman detection apparatus including an apparatus main body and a raman detection assisting device, wherein the method includes: inserting a plurality of Raman chips into the Raman detection auxiliary device to enable the current Raman chip to be detected to be located at the sample position of the equipment main body; performing Raman spectrum measurement on the current Raman chip to be detected through the equipment main body; and after the Raman spectrum measurement of the current Raman chip to be detected is finished, transmitting the next Raman chip in the plurality of Raman chips to the sample position so as to carry out the Raman spectrum measurement of the next Raman chip.
According to an embodiment of the present disclosure, the method further comprises: and reading the chip information of the current Raman chip to be detected.
According to an embodiment of the present disclosure, the method further comprises: and judging the effectiveness of the current Raman chip to be detected according to the chip information.
In the raman detection auxiliary device, the raman detection apparatus and the method in some embodiments of the present disclosure, the plurality of raman chips can be simultaneously placed in batch through the insertion opening of the raman detection auxiliary device, and the plurality of inserted raman chips are sequentially transferred to the predetermined position through the transfer mechanism in the raman detection auxiliary device, so that the raman detection apparatus can be used for automatically realizing batch detection of the plurality of raman chips, and the raman detection efficiency is improved.
Drawings
Fig. 1 is a schematic view of a raman detection assistance device according to one embodiment of the present disclosure;
fig. 2 is a schematic view of a raman detection assistance device according to another embodiment of the present disclosure;
fig. 3 is a schematic diagram of a raman detection apparatus according to one embodiment of the present disclosure;
fig. 4 is a raman chip according to one embodiment of the present disclosure;
FIG. 5 is a chip placement member according to one embodiment of the present disclosure;
FIG. 6 is a chip placement member according to another embodiment of the present disclosure;
fig. 7 is a schematic diagram of a raman detection apparatus according to another embodiment of the present disclosure;
fig. 8 is a schematic diagram of a raman detection apparatus according to yet another embodiment of the present disclosure;
fig. 9 is a flow chart of a raman detection method according to one embodiment of the present disclosure.
Wherein the reference numerals are:
10. 20: a Raman detection auxiliary device; 12: a transport mechanism; 21: a chip placement member; 22: a mounting member; 211. 212, 213, 214, 215: a groove; 100. 200 and 300: a Raman detection device; 110: a handheld Raman detection spectrometer; 120. 220, 320: a Raman detection auxiliary device; 11. 121, 221: an insertion opening; 122. 222: a Raman chip; 1221: a nanoenhanced active region; 123. 223: an eject button; 111. 211: laser; 112. 212, and (3): a laser focus; 124: a strip; 125: a chain; 210: a micro-lens; 310: a raman-enhanced probe.
Detailed Description
The terms and principles involved in this disclosure are first explained by way of explanation.
The scattering molecule is originally in the ground state, and when an external photon is incident on the molecule, the molecule absorbs a photon, transitions to a virtual energy level, and immediately returns to the ground state to emit a photon, which is rayleigh scattering. If the molecule transitions to a virtual energy level without returning to its original ground state and falls to another higher energy level to emit a photon, the new photon energy of this emission is clearly less than the incident photon energy, which is the raman Stokes line (Stokes), and conversely produces an Anti-Stokes line (Anti-Stokes), which are collectively known as raman lines.
Raman spectroscopy (Raman spectroscopy), is a scattering spectrum. The Raman spectroscopy is an analysis method for analyzing a scattering spectrum with a frequency different from that of incident light to obtain information on molecular vibration and rotation based on a Raman scattering effect found by indian scientists c.v. Raman (man), and is applied to molecular structure research.
The Surface Enhanced Raman Scattering (SERS) technology overcomes the inherent weak signal of the conventional Raman spectroscopy, and can increase the Raman intensity by several orders of magnitude. The enhancement factor can be up to 1014-1015 times, which is enough to detect the Raman signal of a single molecule. SERS can be used for trace material analysis, flow cytometry, and other applications. However, the raman amplification chip is formed by attaching a nano material to a silicon wafer or a quartz wafer, so that the raman amplification chip is not suitable for direct measurement and needs to be designed with a special raman amplification detection device.
For a better understanding of the technical aspects of the present disclosure, reference is made to the following detailed description taken in conjunction with the accompanying drawings.
Fig. 1 is a schematic view of a raman detection assisting apparatus according to an embodiment of the present invention. The raman detection assistance device 10 of the present embodiment includes: an insertion port 11 into which a plurality of raman chips can be inserted (wherein the arrow direction in fig. 1 is a raman chip insertion direction); and a conveying mechanism 12 which can be used for conveying the inserted plurality of Raman chips to a preset position in sequence so that the Raman detection equipment can detect the Raman chips.
According to the auxiliary device for Raman detection provided by the embodiment of the disclosure, a plurality of Raman chips can be simultaneously placed in batch through the insertion opening of the auxiliary device for Raman detection, and the inserted Raman chips are sequentially conveyed to the preset position through the conveying mechanism in the auxiliary device for Raman detection, so that the auxiliary device can be used for assisting Raman detection equipment to automatically realize batch detection of the Raman chips, and the efficiency of Raman detection is improved.
In the embodiment shown in fig. 2, the raman detection assistance device 20 may further include: a chip placing member 21, wherein the chip placing member 21 can be used for placing the plurality of Raman chips in sequence, and the Raman detection auxiliary device 20 is inserted through the insertion opening 11; a mounting member 22, the mounting member 22 being operable to secure the chip placement member 21.
With continued reference to fig. 2, the chip placing member 21 has a plurality of grooves (groove 211, groove 212, groove 213, groove 214, and groove 215) to implement the function of placing the plurality of raman chips in sequence, but the present disclosure is not limited thereto, and any other manner capable of implementing placing the plurality of raman chips may be adopted, and the number of the placed raman chips is merely for illustration.
The raman detection auxiliary device that this embodiment provided to can insert this raman detection auxiliary device's chip and place the order that a piece realized a plurality of raman chips and place, can make this raman detection auxiliary device portable, when needs examine again with this chip place the piece insert can, can place the piece through the installed part with this chip and be fixed in this raman detection auxiliary device simultaneously, can improve the positioning accuracy who examines time.
The embodiment of the present disclosure also provides a raman detection apparatus, which may include an apparatus main body and the raman detection auxiliary device described above. The raman detection device is illustrated by means of figures 3 to 8.
Example 1:
fig. 3 is a schematic diagram of a raman detection apparatus according to an embodiment of the present invention. The present embodiment provides a raman detection apparatus 100, where the raman detection apparatus 100 includes an apparatus main body 110 and a raman detection assisting device 120, and the present embodiment takes the case where the raman detection apparatus is a handheld raman enhancement detection apparatus as an example for illustration. The specific structure of the handheld raman detection spectrometer 110 can refer to the existing handheld raman detection spectrometer, and will not be described in detail herein.
For traditional hand-held Raman enhancement detection equipment, the device is only suitable for detecting liquid and powder and is provided with corresponding accessories, but a Raman enhancement chip does not have a corresponding interface, so that the device is not beneficial to rapid field operation of Raman detection.
In this embodiment, the raman detection assisting device 120 may include an insertion port 121, and the insertion port 121 may be used to insert a plurality of raman chips 122 (although fig. 3 shows only one raman chip, the disclosure is not limited thereto). The direction of the arrow in fig. 3 is the insertion direction of the raman chip 122 into the raman detection assisting device 120.
Specifically, the raman chip 122 may be a raman enhancement chip of a predetermined size. As shown in fig. 4, the raman-enhanced chip 122 may include a nano-enhanced effective area 1221 having a predetermined area, and the nano-enhanced effective area 1221 may be used for placing a sample to be tested. For example, the sample to be tested may be a liquid or a powder.
For example, the raman chip 122 is manufactured according to a standard, the central portion may be the nano-enhanced active area 1221, in the embodiment of the present invention, the nano-enhanced active area 1221 has a predetermined area of at least 1 × 1mm, and the outer dimension thereof is a predetermined size, for example, a standard size of 2 × 2 mm.
In an exemplary embodiment, the chip placement member of the raman detection assistance device 120 may include a band or a chain.
Fig. 5 is a chip placing member according to an embodiment of the invention. As shown in fig. 5, the chip placement member may be a tape 124. A plurality of raman chips 122 may be sequentially disposed in the band 124 (although fig. 5 illustrates raman chip 1, raman chip 2, raman chip 3, raman chip 4, and raman chip 5, the number of raman chips that may be disposed in a specific band at the same time is not limited by the present disclosure, and may be selected according to the application).
Fig. 6 is another chip placement member according to an embodiment of the invention. As shown in fig. 6, the chip placing member may be a chain 125. A plurality of raman chips 122 may be sequentially disposed on the chain 125 (although fig. 6 illustrates raman chip 1, raman chip 2, raman chip 3, raman chip 4, and raman chip 5, the number of raman chips that may be disposed in a specific chain at the same time is not limited by the present disclosure, and may be selected according to the application).
In an embodiment of the present invention, the ribbon 124 or the chain 125 may be a predetermined standard size that is adapted to the size of the predetermined standard-size raman chip 122.
It should be noted that, although the above embodiments are exemplified by a predetermined standard-sized raman chip and a predetermined standard-sized chip placing member, the disclosure is not limited thereto, for example, the chip placing member may further include a size adjusting mechanism that can be adaptively adjusted according to the size of the currently placed raman chip to be detected.
In the embodiment of the present invention, the mounting member for fixing or locking the chip placing member may automatically fix the chip placing member when the chip placing member is inserted into the raman detection assisting device 120, for example, any one of the prior art methods such as a snap fastener may be adopted, and the chip placing member may be taken out from the raman detection assisting device 120 after all the raman chips in the chip placing member are measured.
In this embodiment, the raman detection assisting device 120 may further include a conveying mechanism (not shown in the figure) which may be used to convey the inserted plurality of raman chips 122 to a predetermined position in sequence so that the raman detection apparatus 100 detects the raman chips 122.
Specifically, when the raman detection apparatus 100 is in an operating state, the handheld raman detection spectrometer 110 emits the laser 111, and simultaneously, the focal point 112 of the laser 111 of the raman detection apparatus is focused on the nano-enhanced active area of the raman chip at the predetermined position. That is, when the raman detection assistance device 120 is mounted on the hand-held raman detection spectrometer 110, the nanoenhanced active region of the right central region of the raman chip (e.g., the first raman chip in the ribbon or chain may be selected) in the predetermined position is just at the sample position of the hand-held raman detection spectrometer.
In an exemplary embodiment, the nanoenhanced active region of the raman chip may be a right central region of the raman chip. The present disclosure is not limited thereto.
Specifically, the transport mechanism may include an eject button 123, and the eject button 123 may be used to eject the raman chip 122 from the predetermined position. For example, after the measurement of the raman chip at the predetermined position is completed, the raman chip can be ejected from the predetermined position by pressing the eject button 123 so that the next raman chip enters the predetermined position to perform the measurement of the next raman chip.
In this embodiment, the transfer mechanism may further include a stepping motor (not shown in the figure), and the stepping motor may be configured to sequentially transfer the plurality of raman chips to the predetermined position.
In the embodiment of the invention, an accessory (namely a Raman detection auxiliary device) with a strip/chain capable of placing a plurality of Raman chips at the same time is arranged on an existing handheld Raman detection spectrometer, the accessory can fix the strip/chain, the plurality of Raman chips in the strip/chain can be Raman enhancement chips with a preset size, and the strip/chain can be clamped when inserted into the accessory, so that the laser focus emitted by the handheld Raman detection spectrometer is exactly positioned at the center of a first Raman enhancement chip, and Raman spectrum measurement is carried out on the first Raman enhancement chip. When the measurement is finished, the next Raman spectrum of the Raman enhanced chip can be measured by pressing the pop-up button or automatically stepping through the stepping motor.
In an exemplary embodiment, the raman detection assistance device 120 can further include an accessory metal contact that can be disposed on a contact surface of the raman detection assistance device 120 when in contact with the handheld raman detection spectrometer 110. The raman detection assistance device 120 can also include device metal contacts disposed on the contact surface of the handheld raman detection spectrometer 110 when in contact with the raman detection assistance device 120. Wherein, the accessory metal contacts and the device metal contacts are arranged in a one-to-one correspondence, which can be used for data communication and/or power supply between the raman detection assisting device 120 and the hand-held raman detection spectrometer 110. For example, after the handheld raman detection spectrometer 110 has measured a first raman enhancement chip, a control signal may be sent to the raman detection assisting device 120 through one or more preset metal contacts of the apparatus, the raman detection assisting device 120 is informed of the fact that the first raman enhancement chip has been detected through the control signal, and after the raman detection assisting device 120 receives the control signal, the raman detection assisting device may send a step command to the step motor to control the step motor to step the distance of one raman enhancement chip, for example, to transfer a second raman enhancement chip to the predetermined position, and start the measurement of the second raman enhancement chip.
In the embodiment of the invention, an accessory is arranged at the front end of the handheld Raman detection spectrometer, the accessory is provided with an insertion hole which is in zero fit with a strip/chain for placing a Raman enhancement chip, a strip/chain transmission mechanism is arranged in the accessory, so that after the Raman enhancement chip is inserted into the Raman detection auxiliary device, the laser focus of the handheld Raman detection spectrometer is just positioned in a nano enhancement effective area of the Raman enhancement chip at a preset position, and the edge of the accessory is provided with a plurality of metal contacts which are used for being installed on the handheld Raman detection spectrometer and then supplying power and communicating with the spectrometer.
In an exemplary embodiment, the raman detection assisting apparatus 120 may further include a chip information reading apparatus (not shown in the figure) for reading chip information of the raman chip.
Specifically, the chip information reading device may include a magnetic stripe reading device and/or a two-dimensional code reading device. However, the present disclosure is not limited thereto, and any device that can read chip information may be applied to the embodiments of the present disclosure.
In the embodiment of the present invention, after the raman chip is inserted into the raman detection assisting device 120, the chip information can be read by an electromagnetic (magnetic stripe) or optical (two-dimensional code) method, so as to determine the validity of the raman chip, and the chip information is read before each piece of raman chip is measured.
In the embodiment of the invention, the Raman spectrum measurement is carried out on the Raman chip to obtain the Raman enhanced spectrum, and the Raman enhanced spectrum and the chip information of the corresponding Raman chip read before the measurement can be stored in a file for the material identification of the sample to be detected. And when the current Raman chip is measured, the measured Raman chip is ejected, the next Raman chip is automatically stepped, and the unmeasured Raman chip is led in for Raman spectrum measurement. And (4) until all the Raman chips in the strip/chain are measured, drawing out the strip and waiting for the next measurement.
The raman detection apparatus provided by the embodiments of the present disclosure performs raman spectrum measurement of a raman chip by installing an accessory having a strip/chain capable of placing a plurality of raman chips at the same time at the front end of a current handheld raman detection spectrometer, wherein the accessory can fix the strip/chain with a predetermined size, and a laser focus point is exactly opposite to the center of a certain raman chip. When the Raman chip measurement is completed, the next Raman chip spectrum measurement can be conveniently carried out by stepping the distance of one Raman chip every time through pressing or automatic stepping, so that the application of the Raman chip does not need spatial three-dimensional focusing alignment before measurement every time, the batch operation of a plurality of Raman chips can be realized, and meanwhile, the position accuracy and consistency can be kept better among a plurality of Raman chips measured in the same batch, therefore, the Raman detection equipment improves the speed, consistency and effectiveness of Raman spectrum detection.
Example 2:
fig. 7 is a schematic diagram of a raman detection apparatus according to an embodiment of the present invention. The present embodiment provides a raman detection apparatus, which may include an apparatus main body and a raman detection auxiliary device, and the raman detection apparatus is exemplified as a microscopic raman-enhanced automatic measurement apparatus in the present embodiment. As shown in fig. 7, the micro-raman enhanced automatic measurement apparatus 200 may include a microscope lens 210 (a part of an apparatus main body) and a raman detection assisting device 220. The detailed structure of the microscope head 210 can refer to the existing micro-raman enhanced automatic measuring equipment, and will not be described in detail here.
For the traditional microscopic Raman enhancement automatic measurement equipment, a Raman enhancement chip does not have corresponding accessories, three-dimensional alignment is needed for each measurement, and each detection is time-consuming and labor-consuming.
In this embodiment, the raman detection assisting device 220 may include an insertion port 221, and the insertion port 221 may be used to insert a plurality of raman chips 222 (only one raman chip is shown in fig. 7 for illustration).
Specifically, the raman chip 222 may be a raman enhancement chip of a predetermined size. The Raman enhancement chip can comprise a nano enhancement effective area with a preset area, and the nano enhancement effective area can be used for placing a sample to be detected.
In an exemplary embodiment, the raman detection assistance device 220 may further include: a chip placing member for placing the plurality of raman chips 222 in order, and inserting the raman detection assisting device 220 through the insertion port 221; and the mounting part is used for fixing the chip placing part.
In an exemplary embodiment, the chip placement member includes a tape or a chain. Specifically, refer to fig. 5 and fig. 6, which are not described herein again.
Wherein, the raman detection assisting device 220 may further include a conveying mechanism (not shown in the figure), and the conveying mechanism may be configured to convey the inserted plurality of raman chips 222 to a predetermined position in sequence, so that the raman detection apparatus 200 detects the raman chips 222.
In this embodiment, when the raman detection device 200 is in an operating state, the focal point 212 of the laser 211 of the raman detection device 200 is focused on the nano-enhanced active area of the raman chip 222 at the predetermined position.
In this embodiment, the nanoenhanced active region of the raman chip 222 is a substantially central region of the raman chip 222.
In particular, the transport mechanism may comprise an eject button 223, and the eject button 223 may be used to eject the raman chip from the predetermined position.
Specifically, the transfer mechanism may further include a stepping motor (not shown), and the stepping motor may be configured to sequentially transfer the plurality of raman chips 222 to the predetermined position.
In an exemplary embodiment, the raman detection assisting device 220 may further include an accessory metal contact disposed on a contact surface of the raman detection assisting device 220 for data communication and/or power supply between the raman detection assisting device 220 and the apparatus main body of the micro raman enhanced automatic measuring apparatus.
In an exemplary embodiment, the raman detection device 200 may further include: the device metal contact is arranged on the contact surface of the micro lens 210; wherein the accessory metal contact and the equipment metal contact are correspondingly arranged and used for data communication and/or power supply between the Raman detection auxiliary device and the equipment main body.
In an exemplary embodiment, the raman detection assisting apparatus 220 may further include a chip information reading apparatus (not shown in the figure) for reading chip information of the raman chip.
Specifically, the chip information reading device comprises a magnetic stripe reading device and/or a two-dimensional code reading device.
In the embodiment of the invention, an accessory (namely, a Raman auxiliary detection device) can be made on the objective table part of the existing microscopic Raman spectrometer, the accessory is provided with a chip insertion port which is in zero fit with a strip/chain, a plurality of Raman chips such as Raman enhancement chips can be placed in the strip/chain at the same time, and after the strip/chain is inserted into the accessory, the laser focus of the microscopic Raman spectrometer is just positioned in the nano-enhancement effective area of the Raman enhancement chip at a preset position; after the accessory steps by the distance of one Raman enhancement chip every time, chip information of the corresponding Raman enhancement chip is read through an electromagnetic (magnetic stripe) or optical (two-dimensional code) method, the effectiveness of the corresponding Raman enhancement chip is judged, and relevant information of the corresponding Raman enhancement chip, such as a Raman spectrum before measurement, is stored. And ejecting the measured Raman enhancement chip, automatically stepping the accessory, and introducing the unmeasured Raman enhancement chip into the accessory to perform Raman spectrum measurement. And (4) drawing out the strip/chain until all the Raman enhancement chips are measured, and waiting for the next measurement.
The content that is not described in the embodiment of the present invention may refer to the embodiment shown in fig. 1, and is not described herein again.
The raman detection apparatus provided by the embodiments of the present disclosure is configured to mount an accessory having a strip/chain capable of placing a plurality of raman chips at the same time at the front end of a current microscopic raman detection spectrometer, wherein the accessory can fix the strip/chain with a predetermined size, and a laser focus point is exactly opposite to the center of a certain raman chip to perform raman spectrum measurement of the raman chip. When the Raman chip measurement is completed, the next Raman chip spectrum measurement can be conveniently carried out by stepping the distance of one Raman chip every time through pressing or automatic stepping, so that the application of the Raman chip does not need spatial three-dimensional focusing alignment before measurement every time, the batch operation of a plurality of Raman chips can be realized, and meanwhile, the position accuracy and consistency can be kept better among a plurality of Raman chips measured in the same batch, therefore, the Raman detection equipment improves the speed, consistency and effectiveness of Raman spectrum detection.
Example 3:
fig. 8 is a schematic diagram of a raman detection apparatus according to an embodiment of the present invention. The present embodiment provides a raman detection apparatus 300, where the raman detection apparatus 300 may include a raman enhancement probe 310 (i.e., an apparatus main body) and a raman detection assisting device 320, and the present embodiment is exemplified by taking the raman detection apparatus as a raman enhancement probe. The specific structure of the raman detection assisting device 320 can refer to the raman detection assisting devices 120 and 220 in the above embodiments, and will not be described in detail here.
The Raman probe is used for coupling the laser and an external optical path part of the spectrometer, can improve the optical coupling efficiency and the portability of the use of the Raman spectrometer, and can be changed into a Raman enhancement probe by adding the jack of the Raman chip at the front end of the Raman probe.
In the implementation, a fiber laser signal emitted by a fiber laser is changed into parallel laser through a collimating mirror; parallel laser irradiates the dichroic sheet, so that incident laser is reflected to the collimating lens group at an angle of 45 degrees and is focused to the Raman enhancement chip on the strip/chain; raman signals generated by a sample to be detected are reflected along with laser light, pass through a collimating lens group (above 790nm, transmission), are filtered by laser to remove 99.9%, and then pass through a dichroic sheet; raman signals in the optical signals passing through the dichroic filter pass through the filter set without obstruction (the transmission is carried out above 790 nm), and laser signals are filtered; the Raman signal light is focused to a slit of a spectrometer through a focusing mirror group for next spectroscopic measurement.
It should be noted that the raman detection assisting device in any of the above embodiments, i.e. the accessory, may be a separate accessory or may be directly inherited to an existing raman detection apparatus.
The current Raman spectrometer is designed for visible liquid and powder of human eyes, and for the application of a Raman enhancement chip, the three-dimensional focusing alignment of space is needed before each measurement, if the alignment is not accurate, the condition that Raman signals cannot be measured or the signal to noise ratio is poor can occur, misjudgment occurs, time and labor are consumed, and the consistency is poor. The raman detection apparatus provided by the embodiments of the present disclosure, through the raman detection auxiliary device, mounts an accessory having a strip/chain capable of placing a plurality of raman chips simultaneously at a sample position of a handheld, portable, microscopic or other raman apparatus, where the accessory can fix the strip/chain with a predetermined size, and when the strip/chain is inserted, the accessory can be clamped, and a laser focus point just faces to the center of a certain chip, so as to perform raman spectrum measurement of the current raman chip to be detected. When the Raman spectrum measurement of the current Raman chip to be detected is completed, the next Raman chip spectrum measurement can be conveniently carried out by pressing or automatically stepping the distance of one chip at each time, and the Raman detection equipment improves the speed, consistency and effectiveness of Raman spectrum detection.
Fig. 9 is a flowchart of a raman detection method according to an embodiment of the present invention. The Raman detection method can be applied to Raman detection equipment, and the Raman detection equipment can comprise an equipment main body and a Raman detection auxiliary device. The specific raman detection device may refer to the content described in any of the embodiments of fig. 3 to 8, and is not described herein again.
As shown in fig. 9, the raman detection method may include the following steps.
In step S110, a plurality of raman chips are inserted into the raman detection assisting device, so that the current raman chip to be detected is located at the sample position of the apparatus main body.
In step S120, the raman spectrum measurement is performed on the current raman chip to be detected by the device main body.
In step S130, after the raman spectrum measurement of the current raman chip to be detected is completed, a next raman chip of the plurality of raman chips is transferred to the sample position so as to perform the raman spectrum measurement of the next raman chip.
In an exemplary embodiment, the method may further include: and reading the chip information of the current Raman chip to be detected.
In an exemplary embodiment, the method may further include: and judging the effectiveness of the current Raman chip to be detected according to the chip information.
The raman detection method according to the embodiment of the present disclosure is a method for performing raman spectrum measurement of a raman chip by using an attachment having a strip/chain capable of simultaneously placing a plurality of raman chips, and the attachment can fix the strip/chain having a predetermined size so that a laser focusing point of an apparatus main body of a raman detection apparatus is exactly opposite to a center of a certain raman chip. When the Raman chip measurement is completed, the next Raman chip spectrum measurement can be conveniently carried out by stepping the distance of one Raman chip every time through pressing or automatic stepping, so that the application of the Raman chip does not need spatial three-dimensional focusing alignment before every measurement, the batch operation of a plurality of Raman chips can be realized, and meanwhile, the position accuracy and consistency can be kept better among a plurality of Raman chips measured in the same batch, therefore, the Raman detection method improves the speed, consistency and effectiveness of the Raman spectrum detection.
It is to be understood that the above embodiments are merely exemplary embodiments that are employed to illustrate the principles of the present disclosure, and that the present disclosure is not limited thereto. It will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the disclosure, and these are to be considered as the scope of the disclosure.
Claims (14)
1. A raman detection assistance device, comprising:
an insertion port for inserting a plurality of Raman chips;
a transport mechanism for sequentially transporting the plurality of inserted raman chips to a predetermined position so that the raman detection apparatus detects the raman chip at the predetermined position; after the measurement of the Raman chip at the preset position is finished, the conveying mechanism ejects the Raman chip at the preset position from the preset position, or automatically steps by the distance of one Raman chip, so that the next Raman chip in the plurality of Raman chips enters the preset position to carry out the measurement of the next Raman chip;
a chip placing member including a plurality of grooves or strips or chains for placing the plurality of raman chips in order, the raman detection assisting device being inserted through the insertion port;
and the mounting part is used for fixing the chip placing part.
2. The raman detection assistance device according to claim 1, wherein said transport mechanism comprises: and the ejection button is used for ejecting the Raman chip from the preset position.
3. The raman detection assistance device according to claim 1, wherein said transport mechanism comprises: and the stepping motor is used for sequentially conveying the plurality of Raman chips to the preset position.
4. The raman detection assistance device according to claim 1, further comprising: and the accessory metal contact is arranged on the contact surface of the Raman detection auxiliary device and is used for data communication and/or power supply between the Raman detection auxiliary device and the Raman detection equipment.
5. The Raman detection auxiliary device according to claim 1, wherein the Raman chip is a Raman enhancement chip with a predetermined size, the Raman enhancement chip comprises a nano enhancement effective area with a preset area, and the nano enhancement effective area is used for placing a sample to be detected.
6. The raman detection assistance device according to claim 1, further comprising: and the chip information reading device is used for reading the chip information of the Raman chip.
7. The Raman detection auxiliary apparatus according to claim 6, wherein the chip information reading means includes a magnetic stripe reading means and/or a two-dimensional code reading means.
8. A raman detection device, comprising:
an apparatus main body;
a Raman detection auxiliary apparatus according to any one of claims 1 to 7.
9. The raman detection apparatus of claim 8 wherein a laser focus of the raman detection apparatus is focused on a nanoenhanced active region of the raman chip at the predetermined location when the raman detection apparatus is in an operational state.
10. The raman detection apparatus of claim 9 wherein the nanoenhanced active region of the raman chip is a substantially central region of the raman chip.
11. The raman detection apparatus according to claim 8, characterized in that the raman detection apparatus further comprises:
the equipment metal contact is arranged on the contact surface of the equipment main body; wherein the accessory metal contact of the Raman auxiliary detection device and the equipment metal contact are correspondingly arranged and used for data communication and/or power supply between the Raman auxiliary detection device and the equipment main body.
12. A Raman detection method is applied to Raman detection equipment, the Raman detection equipment comprises an equipment main body and a Raman detection auxiliary device, and is characterized in that the Raman detection auxiliary device comprises an insertion opening for inserting a plurality of Raman chips, a conveying mechanism for conveying the inserted Raman chips to a preset position in sequence so that the Raman chips can be detected by the Raman detection equipment, a chip placing piece for placing the Raman chips in sequence through a plurality of grooves or strips or chains and inserting the Raman detection auxiliary device through the insertion opening, and a mounting piece for fixing the chip placing piece; the method comprises the following steps:
inserting a plurality of Raman chips into the Raman detection auxiliary device to enable the current Raman chip to be detected to be located at the sample position of the equipment main body;
performing Raman spectrum measurement on the current Raman chip to be detected through the equipment main body;
after the raman spectrum measurement of the current raman chip to be detected is completed, ejecting the current raman chip to be detected at the sample position from the sample position through the conveying mechanism, or automatically stepping the distance of one raman chip, and conveying the next raman chip in the plurality of raman chips to the sample position so as to perform the raman spectrum measurement of the next raman chip.
13. A raman detection method according to claim 12, characterized in that said method further comprises:
and reading the chip information of the current Raman chip to be detected.
14. A raman detection method according to claim 13, characterized in that said method further comprises: and judging the effectiveness of the current Raman chip to be detected according to the chip information.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2017/119090 WO2019127131A1 (en) | 2017-12-27 | 2017-12-27 | Auxiliary raman detection apparatus, and raman detection device and method |
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| CN108235732A CN108235732A (en) | 2018-06-29 |
| CN108235732B true CN108235732B (en) | 2021-02-12 |
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| WO (1) | WO2019127131A1 (en) |
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| CN112381899A (en) * | 2020-10-10 | 2021-02-19 | 天津工业大学 | SERS spectrum anti-counterfeiting combined coding method based on compound Raman probe |
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| CN108235732A (en) | 2018-06-29 |
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