CN108227170A - One kind is used for microscopical two-dimensional scanner - Google Patents
One kind is used for microscopical two-dimensional scanner Download PDFInfo
- Publication number
- CN108227170A CN108227170A CN201611141426.4A CN201611141426A CN108227170A CN 108227170 A CN108227170 A CN 108227170A CN 201611141426 A CN201611141426 A CN 201611141426A CN 108227170 A CN108227170 A CN 108227170A
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- CN
- China
- Prior art keywords
- microscopical
- flying
- motor
- speculum
- electrically connected
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Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
- G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
- G02B26/10—Scanning systems
- G02B26/12—Scanning systems using multifaceted mirrors
- G02B26/121—Mechanical drive devices for polygonal mirrors
- G02B26/122—Control of the scanning speed of the polygonal mirror
Abstract
The present invention provides a kind of for microscopical two-dimensional scanner, including flying-spot microscope, control section and two-dimensional scan part, the control section and the two-dimensional scan part are set on the flying-spot microscope, and the control section is set as being electrically connected the flying-spot microscope and the two-dimensional scan part；The control section is set as including outer synchronous signal interface, phaselocked loop and servomotor controller, and the output terminal of the outer synchronous signal interface is electrically connected the input terminal of the phaselocked loop, the output terminal of the phaselocked loop is set as being electrically connected the input terminal of the servomotor controller；The two-dimensional scan part is set as including polygonal mirror, speculum and motor.The present invention is simple in structure using the device, can realize the at the uniform velocity efficiently scanning of microscopical two dimension, to avoid sweep speed it is excessively slow caused by excessive sample exposure and the problems such as dye bleach, while return-trace blanking time without field scan, and can effectively reduce production cost.
Description
Technical field
The present invention relates to scanning device technical fields more particularly to one kind to be used for microscopical two-dimensional scanner.
Background technology
During University Scientific Research and Corporation R ＆ D, microscope is to realize to living body biological sample and its quickly become metaplasia
Manage movable observing ancillary equipment, in order to ensure experimental precision, improve the accuracy of experimental result, at the same can realize efficiently into
Therefore picture, with the continuous development of science and technology, higher requirement is also proposed to microscopical sweep speed, still, existing
There are the following problems in actual use for microscopical scanner：
1st, at present, commercial microscope generally uses resonant frequency to carry out object X-direction for the resonance scanner of 7.8KHz
Scanning can realize the imaging capability of 512x512@30fps, for then needing by compressing lines per picture for 512x32@400+fps
Imaging demand is completed, and areas imaging at this time has been compressed into long and narrow strip, has seriously affected Germicidal efficacy, in addition, altogether
Scanner shake because of its eyeglass inertia and operation principle etc., often limits its sweep speed.
2nd, resonance scanner is operated in open loop situations, and near sinusoidal variation is presented in sweep speed in each row, i.e.,
At the uniform velocity, and then sweep speed is excessively slow at both ends for sweep speed approximation per a line center portion (about 88%), therefore, is seen in experiment
The problems such as excessive sample exposure and dye bleach are easily caused during examining.
Invention content
It is a primary object of the present invention to solve problems of the prior art, provide one kind simple in structure, Neng Goushi
Now microscopical two dimension at the uniform velocity efficiently scanning is asked to avoid the excessively slow caused excessive sample exposure of sweep speed and dye bleach etc.
Topic, while return-trace blanking time without field scan, and can effectively reduce production cost for microscopical two-dimensional scanner.
In order to solve the above technical problems, the technical solution adopted by the present invention is：One kind fills for microscopical two-dimensional scan
It puts, wherein described include flying-spot microscope, control section and two-dimensional scan part for microscopical two-dimensional scanner, it is described
The control section and the two-dimensional scan part are set on flying-spot microscope, and the control section is set as being electrically connected institute
State flying-spot microscope and the two-dimensional scan part；
The control section is set as including outer synchronous signal interface, phaselocked loop and servomotor controller, described outer
The input terminal of portion's synchronizing signal interface is electrically connected the X-axis driver of the flying-spot microscope, and the outer synchronous signal interface
Output terminal be electrically connected the input terminal of the phaselocked loop, the outer synchronous signal interface is for receiving the flying-spot microscope
X-axis driver horizontal-drive signal, then X-axis with the flying-spot microscope is converted by signal is received by the phaselocked loop
The pulse signal of the horizontal-drive signal same-phase of driver, the output terminal of the phaselocked loop are set as being electrically connected the servo electricity
The input terminal of machine controller；
The two-dimensional scan part is set as including polygonal mirror, speculum and motor, and the periphery of the polygonal mirror is uniformly set
Put the speculum, the axial angle between the adjacent speculum is set as 0 degree to 90 degree, and the polygonal mirror is centrally disposed
A positioning hole, is coaxially arranged the output shaft of the motor in the location hole, the motor be set as being electrically connected described in watch
Take electric machine controller.
Further, the phaselocked loop is set as digital servo-control circuit, ensures to receive the X of signal and the flying-spot microscope
The synchronization of the horizontal-drive signal of axis driver.
Further, the servomotor controller is set as amplifying by amplifying circuit the pulse letter of the phaselocked loop
Number, and servomotor controller setting driving current is 0.001A to 1000A.
Further, the polygonal mirror is set as axial symmetry polyhedral structure.
Further, the speculum is disposed as equivalent specifications, and the specification of the speculum, quantity and adjacent institute
State sweep speed and line number that the angle between speculum is disposed as depending on the flying-spot microscope.
Further, the polygonal mirror is set as fixing by metal fixture with the motor, to realize the motor pair
The smooth drive of the polygonal mirror ensures the accuracy of experimental result observation.
Further, the rotating speed of the motor is set as 100rpm to 1000000rpm.
The invention has the advantages and positive effects that：
(1) by the control section on flying-spot microscope and two-dimensional scan part, can realize to living body biological sample and
The efficient observation of its quick variation physiological activity, ensure that experimental precision, while improve the accuracy of experimental result.
(2) cooperation of polygonal mirror and motor set by two-dimensional scan part so that polygonal mirror is under the driving of motor
It forms polygonal rotating mirror and carries out X axis scanning, and polygonal rotating mirror can realize uniform speed scanning, and can effectively avoid using resonance
It is needed during scanning to the both ends of every a line into the defects of horizontal blanking, without being equipped with expensive acousto-optic or electro-optic light modulator
It is cost-effective for laser blanking, while sweep speed is also improved into two orders of magnitude.
(3) pass through the structure design in certain axial angle between the adjacent mirror on polygonal mirror periphery so that by every
The reflection light of a piece of speculum is on different straight lines, so as to carry out the scanning of Y direction to sample, and coordinates multi-panel
X axis scanning of the mirror under motor driving, efficiently completes the two-dimensional scan of entire sample, avoids being equipped with galvanometer progress Y-axis side
To the cost of scanning, further reduce costs.
(4) pass through the setting of the equivalent specifications of speculum and the angle foundation between its specification, quantity and adjacent mirror
Sweep speed and line number determine in flying-spot microscope, ensure the good scanning of the two-dimensional scanner, and cause its applicability more
By force.
Description of the drawings
Fig. 1 is the structure diagram schematic diagram of the present invention.
Fig. 2 is the structure diagram of two-dimensional scan part in Fig. 1.
In figure：10- flying-spot microscopes, 20- control sections, 201- outer synchronous signal interfaces, 202- phaselocked loops, 203- are watched
Take electric machine controller, 30- two-dimensional scans part, 301- polygonal mirrors, 302- speculums, 303- motors, 304- location holes.
Specific embodiment
In order to better understand the present invention, the present invention is further retouched with reference to specific embodiments and the drawings
It states.
As depicted in figs. 1 and 2, it is a kind of for microscopical two-dimensional scanner, including flying-spot microscope 10, control section
20 and two-dimensional scan part 30, setting control section 20 and two-dimensional scan part 30 on flying-spot microscope 10, and control section 20
It is set as being electrically connected flying-spot microscope 10 and two-dimensional scan part 30.
Control section 20 is set as including outer synchronous signal interface 201, phaselocked loop 202 and servomotor controller 203,
The input terminal of outer synchronous signal interface 201 is electrically connected the X-axis driver of flying-spot microscope 10, and the outer synchronous signal connects
The output terminal of mouth 201 is electrically connected the input terminal of phaselocked loop 202, and outer synchronous signal interface 201 is used to receive flying-spot microscope
The horizontal-drive signal of 10 X-axis driver, then X-axis with flying-spot microscope 10 is converted by signal is received by phaselocked loop 202
The pulse signal of the horizontal-drive signal same-phase of driver, the output terminal of phaselocked loop 202 are set as being electrically connected servo motor control
The input terminal of device 203 processed.
Two-dimensional scan part 30 is set as including polygonal mirror 301, speculum 302 and motor 303, the periphery of polygonal mirror 301
It is uniformly arranged speculum 302, the axial angle between adjacent mirror 302 is set as 0 degree to 90 degree, and the polygonal mirror 301
Centrally disposed a positioning hole 304, the output shaft of motor 303 is coaxially arranged in location hole 304, and motor 303 is disposed as electrically
Connect servomotor controller 203, motor 303 is controlled to work by servomotor controller 203 so that motor 303 drive it is more
Face mirror 301 rotates, and realizes the X-axis uniform speed scanning of flying-spot microscope 10, and the adjacent mirror 302 on 301 periphery of polygonal mirror is in
Under the cooperation of certain axial angle setting so that the reflection light on speculum 302 is on different straight lines at this time, so as to right
Y direction is scanned, and is completed the at the uniform velocity efficiently scanning of the whole two dimension of sample, is effectively prevented existing using polygonal rotating mirror
X-axis scans and the cost with reference to caused by galvanometer carries out Y axis scanning is higher, complex structure and other problems.
Phaselocked loop 202 is set as digital servo-control circuit, ensures to receive the row of signal and the X-axis driver of flying-spot microscope 10
The synchronization of drive signal.
Servomotor controller 203 is set as amplifying by amplifying circuit the pulse signal of phaselocked loop 202, and servo electricity
It is 0.001A to 1000A that machine controller 203, which sets driving current,.
Polygonal mirror 301 is set as axial symmetry polyhedral structure.
Speculum 302 is disposed as equivalent specifications, and between the specification of speculum 302, quantity and adjacent mirror 302
Angle be disposed as sweep speed and line number depending on flying-spot microscope 10, such as in order to realize 512x512@1000fps into
Picture, when the rotary speed of motor 303 is existing maximum 60000rpm, i.e. 1000fps, polygonal mirror 301 rotates a circle
Realize the scanning of a frame, and a frame includes 512 rows, it is therefore desirable to 512 speculums are installed on the periphery of polygonal mirror 301, every
The optical scan angle of speculum is 720/512=1.4 degree, then is expanded the scanning angle of X-axis by the cooperation of wide-angle lens
To 15 degree, then the scanning angle of Y-axis also mutually should be 15 degree, at this point, the angle between adjacent mirror 302 is 7.5/512=
0.015 degree.
Polygonal mirror 301 is set as fixing by metal fixture with motor 303, to realize motor 303 to the flat of polygonal mirror 301
Steady driving ensures the accuracy of experimental result observation.
The rotating speed of motor 303 is set as 100rpm to 1000000rpm, and motor 303 uses servo motor, in servo motor
It works under the control of controller 203, there are the characteristics such as electromechanical time constant is small, the linearity is high.
It is simple in structure using provided by the present invention for microscopical two-dimensional scanner, it can realize microscopical two
Dimension at the uniform velocity efficiently scanning, to avoid sweep speed it is excessively slow caused by excessive sample exposure and the problems such as dye bleach, it is while field-free
The return-trace blanking time of scanning, and can effectively reduce production cost.When the device works, outer synchronous signal interface 201 connects
The horizontal-drive signal of the X-axis driver of flying-spot microscope 10 is received, then signal will be received by phaselocked loop 202 and be converted into showing with scanning
The pulse signal of the horizontal-drive signal same-phase of the X-axis driver of micro mirror 10 ensures to receive the X-axis of signal and flying-spot microscope 10
Then the arteries and veins of phaselocked loop 202 is amplified in the synchronization of the horizontal-drive signal of driver by the amplifying circuit of servomotor controller 203
Signal is rushed, the work of motor 303 is controlled by servomotor controller 203, so that motor 303 drives polygonal mirror 301 to rotate, it is real
The X-axis uniform speed scanning of existing flying-spot microscope 10, and the adjacent mirror 302 on 301 periphery of polygonal mirror is set in certain axial angle
Under the cooperation put so that the reflection light on speculum 302 is on different straight lines at this time, so as to be swept to Y direction
It retouches, completing the whole two dimension of sample, at the uniform velocity efficiently scanning, the multi-disc for effectively preventing existing rotating polygon mirror scanner periphery are anti-
It penetrates mirror to set each parallel to rotation axis direction so that reflection light is in same straight line, therefore is only capable of realizing lacking for one-dimensional scanning
It falls into and use polygonal rotating mirror X-axis scans and the cost with reference to caused by galvanometer carries out Y axis scanning is higher, it is complicated etc.
Problem.
The embodiment of the present invention is described in detail above, but the content is only presently preferred embodiments of the present invention,
It should not be construed as limiting the practical range of the present invention.All changes and improvements made in accordance with the scope of the present invention, should all
It still belongs within this patent covering scope.
Claims (7)
1. one kind is used for microscopical two-dimensional scanner, it is characterised in that：It is described to be used for microscopical two-dimensional scanner packet
Flying-spot microscope, control section and two-dimensional scan part are included, the control section and described two are set on the flying-spot microscope
Sweep test is tieed up, and the control section is set as being electrically connected the flying-spot microscope and the two-dimensional scan part；
The control section is set as including outer synchronous signal interface, phaselocked loop and servomotor controller, and the outside is same
The input terminal for walking signaling interface is electrically connected the X-axis driver of the flying-spot microscope, and the outer synchronous signal interface is defeated
Outlet is electrically connected the input terminal of the phaselocked loop, and the output terminal of the phaselocked loop is set as being electrically connected the servo motor control
The input terminal of device processed；
The two-dimensional scan part is set as including polygonal mirror, speculum and motor, and the periphery of the polygonal mirror is uniformly arranged institute
State speculum, the axial angle between the adjacent speculum is set as 0 degree to 90 degree, and the polygonal mirror is centrally disposed certain
Position hole, the output shaft of the motor is coaxially arranged in the location hole, and the motor is set as being electrically connected the servo electricity
Machine controller.
2. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The phaselocked loop is set as
Digital servo-control circuit.
3. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The Serve Motor Control
Device is set as amplifying by amplifying circuit the pulse signal of the phaselocked loop, and servomotor controller setting driving current is
0.001A to 1000A.
4. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The polygonal mirror is set as
Axial symmetry polyhedral structure.
5. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The speculum is respectively provided with
For equivalent specifications, and the angle between the specification of the speculum, quantity and the adjacent speculum is disposed as depending on
The sweep speed and line number of the flying-spot microscope.
6. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The polygonal mirror with it is described
Motor is set as fixing by metal fixture.
7. according to claim 1 be used for microscopical two-dimensional scanner, it is characterised in that：The rotating speed of the motor is set
100rpm is set to 1000000rpm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611141426.4A CN108227170A (en) | 2016-12-12 | 2016-12-12 | One kind is used for microscopical two-dimensional scanner |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611141426.4A CN108227170A (en) | 2016-12-12 | 2016-12-12 | One kind is used for microscopical two-dimensional scanner |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108227170A true CN108227170A (en) | 2018-06-29 |
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ID=62638005
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611141426.4A Pending CN108227170A (en) | 2016-12-12 | 2016-12-12 | One kind is used for microscopical two-dimensional scanner |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113009687A (en) * | 2021-03-31 | 2021-06-22 | 苏州溢博伦光电仪器有限公司 | Optical scanning device |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3864564A (en) * | 1973-09-26 | 1975-02-04 | Corning Glass Works | Acquisition system for slide analysis |
| US4241257A (en) * | 1979-05-24 | 1980-12-23 | Koester Charles J | Scanning microscopic apparatus |
| US20050036667A1 (en) * | 2003-08-15 | 2005-02-17 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
| US20060072191A1 (en) * | 2004-10-06 | 2006-04-06 | Yokogawa Electric Corporation | Confocal microscope |
| CN104767443A (en) * | 2015-01-21 | 2015-07-08 | 田苍 | Servo motor control system |
| WO2016203407A1 (en) * | 2015-06-18 | 2016-12-22 | Socpra Sciences Et Genie S.E.C. | Method and system for acoustically scanning a sample |
| CN206003110U (en) * | 2016-07-27 | 2017-03-08 | 凝辉(天津)科技有限责任公司 | Two-dimentional machinery optical scanner |
-
2016
- 2016-12-12 CN CN201611141426.4A patent/CN108227170A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3864564A (en) * | 1973-09-26 | 1975-02-04 | Corning Glass Works | Acquisition system for slide analysis |
| US4241257A (en) * | 1979-05-24 | 1980-12-23 | Koester Charles J | Scanning microscopic apparatus |
| US20050036667A1 (en) * | 2003-08-15 | 2005-02-17 | Massachusetts Institute Of Technology | Systems and methods for volumetric tissue scanning microscopy |
| US20060072191A1 (en) * | 2004-10-06 | 2006-04-06 | Yokogawa Electric Corporation | Confocal microscope |
| CN104767443A (en) * | 2015-01-21 | 2015-07-08 | 田苍 | Servo motor control system |
| WO2016203407A1 (en) * | 2015-06-18 | 2016-12-22 | Socpra Sciences Et Genie S.E.C. | Method and system for acoustically scanning a sample |
| CN206003110U (en) * | 2016-07-27 | 2017-03-08 | 凝辉(天津)科技有限责任公司 | Two-dimentional machinery optical scanner |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113009687A (en) * | 2021-03-31 | 2021-06-22 | 苏州溢博伦光电仪器有限公司 | Optical scanning device |
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Application publication date: 20180629 |