CN108225871A - Purposes of the UV optical cements in pathological section making - Google Patents
Purposes of the UV optical cements in pathological section making Download PDFInfo
- Publication number
- CN108225871A CN108225871A CN201611166221.1A CN201611166221A CN108225871A CN 108225871 A CN108225871 A CN 108225871A CN 201611166221 A CN201611166221 A CN 201611166221A CN 108225871 A CN108225871 A CN 108225871A
- Authority
- CN
- China
- Prior art keywords
- pathological section
- mounting
- optical cements
- purposes
- optical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004568 cement Substances 0.000 title claims abstract description 36
- 230000001575 pathological effect Effects 0.000 title claims abstract description 32
- 230000003287 optical effect Effects 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000003292 glue Substances 0.000 claims abstract description 10
- 239000011521 glass Substances 0.000 claims abstract description 9
- 239000011248 coating agent Substances 0.000 claims description 7
- 238000000576 coating method Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims 2
- 238000001035 drying Methods 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000007935 neutral effect Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000010827 pathological analysis Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The invention discloses purposes of the UV optical cements as pathological section mounting glue, also disclose a kind of mounting method of pathological section, and step is as follows：(1) dyed pathological section glass slide is taken, coats UV optical cements, covered；(2) ultraviolet light, you can.The method of the present invention substantially reduces the time of chip drying, significantly improves the efficiency of experiment, and application prospect is very excellent.
Description
Technical field
The present invention relates to purposes of the UV optical cements in pathological section making.
Background technology
In the manufacturing process of traditional pathological section, mounting is carried out usually using mounting glue.After the completion of mounting, used envelope
It 4-7 days could be completely dry if the natural drying of piece glue is typically passed through.Before it is parched completely, the pathological section was both unfavorable
In for carrying out full sheet digital scan (easily slide or card), also it is unfavorable for storage (easily and other are sliced stick to each others),
Cause experiment process slow, inefficiency.
It, not only can be with if can find out a kind of method can shorten drying time, and can guarantee pathological section picture quality
The efficiency of pathological diagnosis is improved, cost of labor is also reduced and improves the indoor environment of film-making.Therefore, using new method
Shorten the drying time in pathological section manufacturing process, have great significance for the making of pathological section.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of new methods for preparing pathological section.
Present invention firstly provides purposes of the UV optical cements as pathological section mounting glue.
The present invention also provides purposes of the UV optical cements in pathological section mounting.
The present invention also provides a kind of mounting method of pathological section, step is as follows：
(1) dyed pathological section glass slide is taken, coats UV optical cements, covered；
(2) ultraviolet light, you can.
Wherein, in step (1), when coating UV optical cements, the UV optical cements of coating 2.6-4.5 μ L every square centimeter.
In step (2), the power of the ultraviolet light is 20~100W, and irradiation time is 60~600s.
Wherein, the power of the ultraviolet light is 36W, irradiation time 300s.
The present invention using UV optical cements replace traditional mounting glue be used for pathological section mounting, can with rapid curing, when
Between be only several minutes, compared with a couple of days of conventional method, substantially reduce the time of chip drying, significantly improve the effect of experiment
Rate, potential applicability in clinical practice are very excellent.
The present invention is described in further details below by specific embodiment, but is not the limit to the present invention
System, the above according to the present invention, according to the ordinary technical knowledge and customary means of this field, not departing from, the present invention is above-mentioned
Under the premise of basic fundamental thought, the modification, replacement or change of other diversified forms can also be made.
Description of the drawings
After two tissue-derived staining pathologic sections of Fig. 1 sames complete, the slice of UV optical cement mountings is in 36W
Ultra violet lamp 300 seconds enters digital slices scanner scanning after the drying of mounting glue.The slice of neutral Instant cement mounting is in room temperature
It is lower to place one week, enter digital slices scanner scanning after the drying of mounting glue.It is swept for the slice number of UV optical cement mountings on the left side
Trace designs picture, and the right is the slice Digital scan image of traditional neutral Instant cement mounting.Four row from top to bottom, respectively 3.5 times put
Big image (tissue overall picture), 100 times of enlarged drawings, 200 times of enlarged drawings, 400 times of enlarged drawings.Through 5 pathological diagnosis doctors
Differentiate, the right and left image reaches pathological diagnosis requirement without significant difference.
Specific embodiment
The mounting method of 1 pathological section of the present invention of embodiment
1st, technique
(1) the pathological section glass slide for having completed dyeing, 3.5 microlitres of UV optical cement (Henkel of coating every square centimeter are taken
3106 optical cements of happy Thailand), covered later；
(2) artificial ultraviolet light 300 seconds (uv power 36W), you can.
2nd, it detects
2.1 detection method
The slice being completely dried relatively firmly is rubbed with the hands first with two fingers, should not there is slip between coverslip and glass slide.Hereafter may be used
Slice is erect, it is front and rear to stack, it stores 24 hours, it mutually will not adhesion.
2.2 testing result
By detection, the pathological section that the method for the present invention is prepared has parched, has been used directly for full sheet number and sweeps
It retouches, storage is convenient to.As shown in Figure 1, by Digital scan image, with traditional neutral Instant cement mounting Comparative result, UV
Optical cement mounting image effect does not have difference with traditional neutral Instant cement mounting image effect, and can meet Clinicopathologic Diagnosis will
It asks.
The mounting method of 2 pathological section of the present invention of embodiment
1st, technique
(1) the pathological section glass slide for having completed dyeing, 2.6 microlitres of UV optical cement (Henkel of coating every square centimeter are taken
3106 optical cements of happy Thailand), covered later；
(2) artificial ultraviolet light 60 seconds (uv power 100W), you can.
2nd, it detects
2.1 detection method
The slice being completely dried relatively firmly is rubbed with the hands first with two fingers, should not there is slip between coverslip and glass slide.Hereafter may be used
Slice is erect, it is front and rear to stack, it stores 24 hours, it mutually will not adhesion.
2.2 testing result
By detection, the pathological section that the method for the present invention is prepared has parched, has been used directly for full sheet number and sweeps
It retouches, storage is convenient to.By Digital scan image, with traditional neutral Instant cement mounting Comparative result, UV optical cement mountings
Image effect does not have difference with traditional neutral Instant cement mounting image effect, can meet Clinicopathologic Diagnosis requirement.
The mounting method of 3 pathological section of the present invention of embodiment
1st, technique
(1) the pathological section glass slide for having completed dyeing, 4.5 microlitres of UV optical cement (Henkel of coating every square centimeter are taken
3106 optical cements of happy Thailand), covered later；
(2) artificial ultraviolet light 600 seconds (uv power 20W), you can.
2nd, it detects
2.1 detection method
The slice being completely dried relatively firmly is rubbed with the hands first with two fingers, should not there is slip between coverslip and glass slide.Hereafter may be used
Slice is erect, it is front and rear to stack, it stores 24 hours, it mutually will not adhesion.
2.2 testing result
By detection, the pathological section that the method for the present invention is prepared has parched, has been used directly for full sheet number and sweeps
It retouches, storage is convenient to.By Digital scan image, with traditional neutral Instant cement mounting Comparative result, UV optical cement mountings
Image effect does not have difference with traditional neutral Instant cement mounting image effect, can meet Clinicopathologic Diagnosis requirement.
To sum up, the present invention replaces traditional mounting glue to be used for the mounting of pathological section using UV optical cements, can quickly consolidate
Change, the time is only several minutes, compared with a couple of days of conventional method, substantially reduces the time of chip drying, significantly improves reality
The efficiency tested, and the chipping qualities prepared does not have difference with conventional flap glue, and potential applicability in clinical practice is very excellent.
Claims (6)
- Purposes of the 1.UV optical cements as pathological section mounting glue.
- Purposes of the 2.UV optical cements in pathological section mounting.
- 3. a kind of mounting method of pathological section, it is characterised in that：Step is as follows：(1) dyed pathological section glass slide is taken, coats UV optical cements, covered；(2) ultraviolet light, you can.
- 4. mounting method according to claim 3, it is characterised in that：In step (1), when coating UV optical cements, every square The UV optical cements of centimetre coating 2.6-4.5 μ L.
- 5. preparation method according to claim 3, it is characterised in that：In step (2), the power of the ultraviolet light is 20~100W, irradiation time are 60~600s.
- 6. preparation method according to claim 5, it is characterised in that：The power of the ultraviolet light is 36W, during irradiation Between be 300s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611166221.1A CN108225871A (en) | 2016-12-13 | 2016-12-13 | Purposes of the UV optical cements in pathological section making |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611166221.1A CN108225871A (en) | 2016-12-13 | 2016-12-13 | Purposes of the UV optical cements in pathological section making |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108225871A true CN108225871A (en) | 2018-06-29 |
Family
ID=62651630
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611166221.1A Pending CN108225871A (en) | 2016-12-13 | 2016-12-13 | Purposes of the UV optical cements in pathological section making |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108225871A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749634A (en) * | 2019-02-25 | 2019-05-14 | 暨南大学 | A kind of quick mountant and the preparation method and application thereof based on photocuring |
| CN110398401A (en) * | 2019-07-12 | 2019-11-01 | 上海晶铸生物科技有限公司 | A kind of method of Thinprep pap test film-making |
| CN110655873A (en) * | 2019-10-21 | 2020-01-07 | 四川大学华西医院 | UV optical adhesive and application thereof as pathological section mounting adhesive |
| CN112577794A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Method for preparing film-taking enriched bacteria by shadowless adhesive and application thereof |
| CN112985952A (en) * | 2021-02-20 | 2021-06-18 | 山东骏腾医疗科技有限公司 | Novel pathological sealing sheet material |
| CN113405880A (en) * | 2021-05-21 | 2021-09-17 | 刘济忠 | Pathological specimen sealing liquid and preparation and sealing methods thereof |
| CN113405870A (en) * | 2021-06-16 | 2021-09-17 | 上海蓝盎医学科技发展有限公司 | New method for sealing sheet |
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| CN1865432A (en) * | 2005-05-20 | 2006-11-22 | 麦克奥迪实业集团有限公司 | Cell collection method after laser microdissection |
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2016
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| CN1865432A (en) * | 2005-05-20 | 2006-11-22 | 麦克奥迪实业集团有限公司 | Cell collection method after laser microdissection |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109749634A (en) * | 2019-02-25 | 2019-05-14 | 暨南大学 | A kind of quick mountant and the preparation method and application thereof based on photocuring |
| CN109749634B (en) * | 2019-02-25 | 2021-07-23 | 暨南大学 | Rapid encapsulating tablet based on photocuring and preparation method and application thereof |
| CN110398401A (en) * | 2019-07-12 | 2019-11-01 | 上海晶铸生物科技有限公司 | A kind of method of Thinprep pap test film-making |
| CN112577794A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Method for preparing film-taking enriched bacteria by shadowless adhesive and application thereof |
| CN110655873A (en) * | 2019-10-21 | 2020-01-07 | 四川大学华西医院 | UV optical adhesive and application thereof as pathological section mounting adhesive |
| WO2021078125A1 (en) * | 2019-10-21 | 2021-04-29 | 四川大学华西医院 | Uv optical adhesive and use thereof as section sealing adhesive for pathological section |
| CN110655873B (en) * | 2019-10-21 | 2021-06-04 | 四川大学华西医院 | UV optical adhesive and application thereof as pathological section mounting adhesive |
| CN112985952A (en) * | 2021-02-20 | 2021-06-18 | 山东骏腾医疗科技有限公司 | Novel pathological sealing sheet material |
| CN113405880A (en) * | 2021-05-21 | 2021-09-17 | 刘济忠 | Pathological specimen sealing liquid and preparation and sealing methods thereof |
| CN113405870A (en) * | 2021-06-16 | 2021-09-17 | 上海蓝盎医学科技发展有限公司 | New method for sealing sheet |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180629 |
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| RJ01 | Rejection of invention patent application after publication |