CN108220202A - A kind of method that biogas biologic recycling utilizes - Google Patents
A kind of method that biogas biologic recycling utilizes Download PDFInfo
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- CN108220202A CN108220202A CN201810169103.9A CN201810169103A CN108220202A CN 108220202 A CN108220202 A CN 108220202A CN 201810169103 A CN201810169103 A CN 201810169103A CN 108220202 A CN108220202 A CN 108220202A
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Abstract
The present invention discloses a kind of method that biogas biologic recycling utilizes, and can pass through High Density Cultivation aerobic methane backeria aerobic methane backeria synthesising biological matter and the other-end product of continuously ventilating using biogas as fermentation substrate using this method.Present invention can apply to the extensive high efficiency productions of aerobic methane backeria and its active material.By optimizing aerobic methane backeria condition of culture and medium component, the bioavailable efficiency of biogas is improved, so as to make 20 40g/L of organism dry weight concentration, dry cell weight yield 10 20g/L/ days that biogas biosynthesis obtains, 2 10g/L of production concentration.Biological utilisation for biogas provides a kind of method of high-efficiency resource recycling.
Description
Technical field
The present invention relates to a kind of methods by bioanalysis recycling biogas, belong to biological chemical field.
Background technology
In recent years, the change for resulting in environment is significantly increased in firedamp drainage amount, is brought for the mankind and other species huge
Big harm.Research shows that the growth of demographic and economic increases the consumption to the energy, it is contemplated that the middle of this century, consumption
By at least current twice.Currently, existing lot of research shows that fossil energy consumption, firedamp drainage and global environment become
Relationship between change, correlated results point out that environmental change influences not allowing to neglect caused by society and economic dispatch various aspects simultaneously
Depending on.
Biogas mainly includes the carbon dioxide and methane based on one gas of carbon.Research shows that cause the natural pond of global warming
In gas, the proportion of methane has reached 20%, and is calculated with 20 terms, and the global warming potential of methane is 86 times of carbon dioxide.
Methane derives from a wealth of sources, and garbage loading embeading factory, sewage treatment plant, animal farm etc. are mainly present in the form of biogas can carry out micro- life
Object anaerobic degradation fermentation place.
China attaches great importance to Methane Resources in recent years, and with its production technology constantly improve, biogas has become a kind of honest and clean
Valency, sufficient carbon source.But the current a large amount of biogas utilizations in China are mainly burnt based on still being utilized with low-grade heat by cogeneration of heat and power
Methane in biogas generates thermal energy and electric energy.However this inefficient method using biogas, not only greatly reduce the valency of biogas
Value, and the carbon dioxide that combustion of methane generates causes secondary pollution.Therefore being continuously increased with biogas output, biogas
High-end utilization ways are badly in need of constantly extension and supplement.
Aerobic methane backeria is a kind of microorganism that can be using methane as sole carbon source and energy source, distinctive in vivo
Biogas can be biologically converted into including microbial grease, long chain fatty acids, glycogen, poly-β-hydroxybutyric acid, extracellular polymer by biological enzyme
A variety of high value active materials including object, tetrahydropyrimidine, epoxides, astaxanthin, methanol, mycoprotein etc..Therefore, mesh
Preceding aerobic methane backeria has a wide range of applications in food, medicine, feed, all various aspects such as environmental protection and bioenergy.
But aerobic methane backeria requires condition of culture extremely harsh.Not optimised growth conditions and condition of culture be difficult
Realize that the methane in aerobic methane backeria Efficient Conversion biogas generates the purpose of high value added product.It is compared to and is using carbohydrate
The microorganism of carbon substrate, the sole carbon source and energy source of aerobic methane backeria are biogas.Gaseous carbon sources have relative to carbohydrate
It is not easy to be controlled and the characteristics of transformation efficiency is low.It can not such as be realized without the optimization of condition of culture and efficiently realize height using biogas
The purpose of density fermentation, so as to lose the prospect of industrialized production.
Invention content
The present invention for current Methane Resourcesization using technology existing during insufficient and bioconversion methane and
The jejune problem of condition by optimizing aerobic methane backeria condition of culture and medium component, improves the utilization ratio of biogas.
Culture aerobic methane backeria is carried out using the training method continuously ventilated, biogas is continuously passed through simultaneously in incubation
Exclusive segment bacterium solution, while the Optimal Medium for adding respective volume carries out subsequent technology culture.
The aerobic methane backeria is selected from：Methylomonas pelagica,Pseudomonas methanica,
Methylosinus trichosporium OB3b,Methylosinus sporium,Methylobacter
modestohalophilus,Methylocystis parvus,Methylomicrobium specie,Methylomonas
methanica,Methylomonas albus,Methylobacter alcaliphilus,Methylococcus
capsulatus(Bath),Methylobacter album,Methylobacterium extorquens,
Methylobacter capsulatus,Methylobacterium organophilum,Methylomonas specie,
Methylomicrobium buryatense,Methylomicrobium alcaliphilum,Methylobacterium
radiotolerans,Methylocella silvestris,Methylobacterium populi,
Methylacidiphilum infernorum,Methylibium petroleiphilum,Methylocystis GB25,
Methylobacterium chloromethanicum,Verrucomicrobia phylum,Methylobacterium
The compound mixed bacterial of nodulans, Methylomicrobium alcaliphilum or above-mentioned strain.
The aerobic methane backeria in culture of continuous cultivation, controls bioreactor using biogas as sole carbon source
Speed of agitator is 200-1000 revs/min, and biogas is continuously passed through bioreactor with the flow of 0.5-3vvm, and keep cultivating
Temperature is 20-35 DEG C, and the pH for adjusting culture solution in culture medium and bioreactor is 5.0-10.0.
The culture medium is made of nitrogen source, carbonate, inorganic salts, trace element and water
The continuous culture is in revolving bottle, mechanical agitation type, gas-lifting type, bubble type or the circulating biological respinse of biomembrane
It is carried out in device.
The biomass and other-end product include drying thalline, microbial grease, mycoprotein, glycogen and poly- β-
Hydroxybutyric acid.
The main component of the liquid medium is：MgSO4·7H20.2~5.0 g/l of O, CaCl2·6H2O0.002~
0.2 g/l, KNO30.1-10.0 g/l, NaNO30.5-5.0 g/l, K2SO40.1-1.0 g/l, NaCl 1.0-
10.0 g/l, KH2PO40.1~1.0 g/l, Na2HPO40.1~1.0 g/l, NaHCO30.5-5.0 g/l, Na2CO3
1.0-5.0 g/l, 0.5~5 g/l of Na-EDTA, FeSO4·7H20.1~4 g/l of O, ZnSO47H2O 0.01~1.2
G/l, MnSO4·4H2O 0.1-0.5 g/l, MnCl2·4H20.003~0.1 g/l of O, H3BO30.02~0.12 gram/
It rises, CoCl2·6H2O0.02~0.4 g/l, CuCl2·2H2O 0.02-0.6 g/l, CaCl2·2H2O 0.001-0/1 gram/
It rises, NiCl2·6H20.002~0.1 g/l of O, Na2MoO4·2H20.003~0.1 g/l of O, CuSO4·5H2O0.5~5
G/l, 0.05-1.0 g/l of KI (potassium iodide) and 1mM H2SO40.1-2ml, the composition of chelant ferrous solution is FeSO4·7H2O
0.5-10.0 g/l, FeCl31.0-5.0 g/l, HCl 1.0-5.0ml and Na2- EDTA 0.5-4.0 g/l are realized highly dense
The degree continuous culture aerobic methane backeria.
The biomass dry weight that the aerobic methane backeria obtains after high density is continuously cultivated reaches 20-40g/L.
For the present invention by optimizing aerobic methane backeria condition of culture and medium component, that improves methane in sewage gas utilizes effect
Rate, so as to make organism dry weight concentration 20-40g/L, the dry cell weight yield 10-20g/L/ days, product that methane biosynthesis obtains
Concentration 2-10g/L.Utilization for biogas provides a kind of method of high-performance bio method recycling.
Description of the drawings
Fig. 1 is embodiment 1, biomass growth rate curve graph in example 2 and example 3.Each component meaning in Fig. 1：#A, #B and #C
Represent above-mentioned three kinds of different culture medias；OD represents thalline absorbance value；Time represents the time hour (h).
Fig. 2 is embodiment 1, cell dry weight concentration curve graph in example 2 and example 3.Each component meaning in Fig. 2：#A, #B and #C
Represent above-mentioned three kinds of different culture medias；DCW represents cell dry weight concentration gram per liter (g/L) in figure；Time represents the time hour
(h)。
Fig. 3 is embodiment 1, bio-oil content curve graph in example 2 and example 3.Each component meaning in Fig. 3：#A, #B and #C
Represent above-mentioned three kinds of different culture medias；Lipid content represent the ratio that bio-oil (i.e. product) accounts for dry cell weight；Time
Represent the time hour (h).
Fig. 4 is biomass growth rate, cell dry weight concentration and glycogen concentration curve graph in embodiment 4.Each component in Fig. 4
Meaning：；OD represents thalline absorbance value；DCW represents cell dry weight concentration gram per liter (g/L)；Glycogen represents glycogen concentration
Gram per liter (g/L)；Time represents the time hour (h).
Specific embodiment
The present invention is described in further detail below by specific embodiment, but the aerobic methane backeria of the present patent application
Including but not limited to Methylomonas pelagica, Pseudomonas methanica, Methylosinus
trichosporium OB3b,Methylosinus sporium,Methylobacter modestohalophilus,
Methylocystis parvus,Methylomicrobium specie,Methylomonas methanica,
Methylomonas albus,Methylobacter alcaliphilus,Methylococcus capsulatus(Bath),
Methylobacter album,Methylobacterium extorquens,Methylobacter capsulatus,
Methylobacterium organophilum,Methylomonas specie,Methylomicrobium
buryatense,Methylomicrobium alcaliphilum,Methylobacterium radiotolerans,
Methylocella silvestris,Methylobacterium populi,Methylacidiphilum infernorum,
Methylibium petroleiphilum,Methylocystis GB25,Methylobacterium
chloromethanicum,Verrucomicrobia phylum,Methylobacterium nodulans,
The compound mixed bacterial of Methylomicrobium alcaliphilum or above-mentioned strain.
In a preferred embodiment, the present invention uses Methylomicrobium buryatense,
For Methylomicrobium alcaliphilum, Methylosinus trichosporium OB3b mixed bacterials, say
The specific embodiment of the bright present invention.In the case of without prejudice to spirit of the invention and essence, to the method for the present invention, step or item
The modifications or substitutions that part is made, all belong to the scope of the present invention.
Embodiment 1：
Prepare liquid medium #A and liquid microelement #1 (its composition is shown in Tables 1 and 2), by the liquid medium accordingly prepared with
Liquid microelement is added in bioreactor, and to working volume, the Liquid Fill coefficient of bioreactor is supplement pure water
0.6.The continuous culture of mixed bacterial carries out in the bioreactor of revolving bottle, mechanical agitation type, gas-lifting type or bubble type.
In preferred example, using mechanical agitation type bioreactor.
Steam sterilizing is carried out to the bioreactor for having filled liquid (121 DEG C maintain 30 minutes).When temperature is down to 30 DEG C, press
8% access seed liquor of dress liquid product starts continuously to cultivate.
In incubation, suitable condition of culture must be controlled to realize the High Density Cultivation of mixed bacterial.Preferred real
In example, control 500 revs/min of speed of agitator and biogas is passed through with the flow of 0.8vvm, oxyty is kept to be more than 10% as possible.
Controlled at 28 DEG C, using 4mol/L NaOH and 4mol/L H2SO4It is 9.0 to regulate and control pH.
After continuous culture starts, liquid medium #A and liquid microelement #1 can realize High Density Cultivation.OD peaks reach
48.5 (see Fig. 1), cell dry weight concentration reach 19.4g/L (see Fig. 2), and product fat content is 11% (see Fig. 3).
Embodiment 2
Described in Tables 1 and 2, liquid medium #B and liquid microelement #2, other condition of culture and patent requirements are prepared
With example 1.
After continuous culture starts, liquid medium #B and liquid microelement #2 provide OD peaks up to 9.6 (see Fig. 1), cell
Dry weight concentrations reach 3.9g/L (see Fig. 2), and product fat content is 8% (see Fig. 3).
Embodiment 3
Described in Tables 1 and 2, liquid medium #C and liquid microelement #3, other condition of culture and patent requirements are prepared
With example 1.
After continuous culture starts, liquid medium #C and liquid microelement #3 can realize High Density Cultivation.OD peaks are big
52.7 (see Fig. 1), cell dry weight concentration reach 21.1g/L (see Fig. 2), and product fat content is 15% (see Fig. 3).
Embodiment 4：
Prepare liquid medium #C and liquid microelement #2 (its composition is shown in Tables 1 and 2), by the liquid medium accordingly prepared with
Liquid microelement is added in bioreactor, and to working volume, the Liquid Fill coefficient of bioreactor is supplement pure water
0.4.The continuous culture of mixed bacterial carries out in the bioreactor of revolving bottle, mechanical agitation type, gas-lifting type or bubble type.
In preferred example, using mechanical agitation type bioreactor.
Steam sterilizing is carried out to the bioreactor for having filled liquid (121 DEG C maintain 30 minutes).When temperature is down to 30 DEG C, press
10% access seed liquor of dress liquid product starts continuously to cultivate.
In incubation, suitable condition of culture must be controlled to realize the High Density Cultivation of mixed bacterial.Preferred real
In example, control 1000 revs/min of speed of agitator and biogas is passed through with the flow of 2vvm, oxyty is kept to be more than 5% as possible.Control
Temperature processed is 30 DEG C, using 2mol/L NaOH and 2mol/L H2SO4It is 8 to regulate and control pH.
After continuous culture starts, liquid medium #A and liquid microelement #3 can realize High Density Cultivation biosynthesis sugar
It is former.OD peaks reach 25g/L (see Fig. 4), glycogen concentration 10g/L up to 62 (see Fig. 4), cell dry weight concentration (see Fig. 4).
1 liquid medium constituent of table
2 trace element solution constituent of table
From above-described embodiment as can be seen that by optimizing aerobic methane backeria condition of culture and medium component, natural pond is improved
The utilization ratio of methane in gas, so as to which the organism dry weight concentration that biogas biosynthesis obtains be made to be produced not less than 20g/L, dry cell weight
Rate was not less than 2g/L not less than 10g/L/ days, production concentration.Utilization for biogas provides a kind of high-performance bio method recycling
Method.
Claims (7)
1. a kind of method that biogas biologic recycling utilizes, which is characterized in that by the use of aerobic methane backeria using biogas as uniquely
Carbon source catalyzes and synthesizes biogas for biomass and other-end product under high-density culturing condition, in culture of continuous cultivation,
The speed of agitator for controlling bioreactor is 200-1200 revs/min, will be continuously passed through with the flow of 0.5-3vvm containing biogas
Bioreactor, and it is 20-35 DEG C to keep cultivation temperature, and the pH for adjusting culture solution in culture medium and bioreactor is 5.0-
10.0。
2. the method that a kind of biogas biologic recycling according to claim 1 utilizes, which is characterized in that the aerobic first
Alkane bacterium is selected from：Methylomonas pelagica,Pseudomonas methanica,Methylosinus
trichosporium OB3b,Methylosinus sporium,Methylobacter modestohalophilus,
Methylocystis parvus,Methylomicrobium specie,Methylomonas methanica,
Methylomonas albus,Methylobacter alcaliphilus,Methylococcus capsulatus(Bath),
Methylobacter album,Methylobacterium extorquens,Methylobacter capsulatus,
Methylobacterium organophilum,Methylomonas specie,Methylomicrobium
buryatense,Methylomicrobium alcaliphilum,Methylobacterium radiotolerans,
Methylocella silvestris,Methylobacterium populi,Methylacidiphilum infernorum,
Methylibium petroleiphilum,Methylocystis GB25,Methylobacterium
chloromethanicum,Verrucomicrobia phylum,Methylobacterium nodulans,
The compound mixed bacterial of Methylomicrobium alcaliphilum or above-mentioned strain.
3. the method that a kind of biogas biologic recycling according to claim 1 utilizes, which is characterized in that the biomass and
Other-end product includes drying thalline, microbial grease, mycoprotein, glycogen and poly-β-hydroxybutyric acid.
4. the method that a kind of biogas biologic recycling according to claim 1 utilizes, which is characterized in that High Density Cultivation mistake
Journey is that the High Density Cultivation of aerobic methane backeria is carried out using the training method continuously ventilated, and natural pond is continuously passed through in incubation
Gas simultaneously excludes part bacterium solution in bioreactor, while the liquid medium realization for adding respective volume subsequently continues to cultivate.
5. the method that a kind of biogas biologic recycling according to claim 1 utilizes, which is characterized in that the continuous culture
It is carried out in revolving bottle, mechanical agitation type, gas-lifting type, bubble type or the circulating bioreactor of biomembrane.
6. the method that a kind of biogas biologic recycling according to claim 1 utilizes, which is characterized in that the culture medium by
Nitrogen source, carbonate, inorganic salts, trace element and water composition.
7. the method that a kind of biogas biologic recycling according to claim 1 utilizes, it is characterised in that the master of liquid medium
It forms and is：MgSO4·7H20.2~5.0 g/l of O, CaCl2·6H20.002~0.2 g/l of O, KNO30.1-10.0 grams/
It rises, NaNO30.5-5.0 g/l, K2SO40.1-1.0 g/l, NaCl 1.0-10.0 g/l, KH2PO40.1~1.0 gram/
It rises, Na2HPO40.1~1.0 g/l, NaHCO30.5-5.0 g/l, Na2CO31.0-5.0 g/l, Na2- EDTA 0.5~
5 g/l, FeSO4·7H20.1~4 g/l of O, ZnSO4·7H20.01~1.2 g/l of O, MnSO4·4H2O 0.1-0.5
G/l, MnCl2·4H20.003~0.1 g/l of O, H3BO30.02~0.12 g/l, CoCl2·6H2O 0.02~0.4
G/l, CuCl2·2H2O 0.02-0.6 g/l, CaCl2·2H2O 0.001-0/1 g/l, NiCl2·6H2O 0.002~
0.1 g/l, Na2MoO4·2H20.003~0.1 g/l of O, CuSO4·5H20.5~5 g/l of O, KI (potassium iodide) 0.05-
1.0 g/l and 1mM H2SO40.1-2ml, the composition of chelant ferrous solution is FeSO4·7H2O 0.5-10.0 g/l, FeCl3
1.0-5.0 g/l, HCl 1.0-5.0ml and Na2- EDTA 0.5-4.0 g/l, it is good described in the continuous culture of high density to realize
Oxygen methane backeria.
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| CN113862205A (en) * | 2021-05-13 | 2021-12-31 | 西安交通大学 | Methanotrophic engineering bacterium for producing hydroxyl tetrahydropyrimidine |
| CN114045235A (en) * | 2021-11-04 | 2022-02-15 | 西安交通大学 | Method for producing single-cell protein and fermentable sugar by using methanotrophic bacteria |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113862205A (en) * | 2021-05-13 | 2021-12-31 | 西安交通大学 | Methanotrophic engineering bacterium for producing hydroxyl tetrahydropyrimidine |
| CN113862205B (en) * | 2021-05-13 | 2023-08-01 | 西安交通大学 | Methanotrophic engineering bacteria for producing hydroxy tetrahydropyrimidine |
| CN114045235A (en) * | 2021-11-04 | 2022-02-15 | 西安交通大学 | Method for producing single-cell protein and fermentable sugar by using methanotrophic bacteria |
| CN114045235B (en) * | 2021-11-04 | 2022-11-08 | 西安交通大学 | Method for producing single-cell protein and fermentable sugar by using methanotrophic bacteria |
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