CN108210416A - facial mask and preparation method thereof - Google Patents

facial mask and preparation method thereof Download PDF

Info

Publication number
CN108210416A
CN108210416A CN201810108057.1A CN201810108057A CN108210416A CN 108210416 A CN108210416 A CN 108210416A CN 201810108057 A CN201810108057 A CN 201810108057A CN 108210416 A CN108210416 A CN 108210416A
Authority
CN
China
Prior art keywords
facial mask
cuttlefish
parts
preparation
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201810108057.1A
Other languages
Chinese (zh)
Inventor
陈海佳
葛啸虎
王飞
王一飞
王小燕
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Saliai StemCell Science and Technology Co Ltd
Original Assignee
Guangzhou Saliai StemCell Science and Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Saliai StemCell Science and Technology Co Ltd filed Critical Guangzhou Saliai StemCell Science and Technology Co Ltd
Priority to CN201810108057.1A priority Critical patent/CN108210416A/en
Publication of CN108210416A publication Critical patent/CN108210416A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dermatology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The present invention relates to a kind of facial masks and preparation method thereof.Wherein, facial mask includes face paper and facial mask liquid, and by weight, facial mask liquid is made including following raw materials：10 20 parts of cuttlefish polypeptide powders；15 20 parts of moisturizer；5 15 parts of Katsumadai seed extracts；And 20 40 parts of water.Above-mentioned facial mask, with good synergistic effect, can effectively remove interior free yl (O with senile-resistant efficacy, cuttlefish polypeptide powder and Katsumadai seed extract2‑、H2O2, ROO and OH etc.), inoxidizability is high, and non-stimulated to skin, has good moisture-keeping efficacy.

Description

Facial mask and preparation method thereof
Technical field
The present invention relates to cosmetic technical field, more particularly to a kind of facial mask and preparation method thereof.
Background technology
Modern medicine shows that the production quantity of skin aging and internal oxygen radical is closely related.In order to prevent skin aging, Improve wrinkle and reach skin-whitening and skin moisturization, need effectively to remove the activity generated by natural or non-natural reason Oxygen (oxygen radical).
Traditional facial mask does not have anti-aging function mostly, and the facial mask that minority has anti-aging, which is also mostly that addition is artificial, to be closed Into antioxidant, it is big to skin irritation, allergic symptom can occur even with person's face.
Invention content
Based on this, it is necessary in view of the above-mentioned problems, providing a kind of anti-aging face non-stimulated to skin, containing natural extract Film.
A kind of facial mask, the facial mask include face paper and facial mask liquid, and by weight, the facial mask liquid includes following raw materials It is made：
10-20 parts of cuttlefish polypeptide powders；
15-20 parts of moisturizer；
5-15 parts of Katsumadai seed extracts；
And 20-40 parts of water.
Above-mentioned facial mask with senile-resistant efficacy, cuttlefish polypeptide powder and Katsumadai seed extract with good synergistic effect, can Effectively to remove interior free yl (O2-、H2O2, ROO and-OH etc.), inoxidizability is high, and non-stimulated to skin, has good Moisture-keeping efficacy.
The face paper is silk mask cloth or alginate fibre in one of the embodiments,.
The moisturizer is ceramide in one of the embodiments,.
The molecular weight of the cuttlefish polypeptide powder is 400Da-1600Da in one of the embodiments,.
The cuttlefish polypeptide powder includes following several peptide fragments in one of the embodiments,：
The amino acid sequence of peptide fragment M1 is SEQ ID NO.1：Tyr-Leu-Ser-Trp-Lys-Leu；
The amino acid sequence of peptide fragment M2 is SEQ ID NO.2：Leu-Leu-Thr-Asp-Val-Tyr-Gln；
The amino acid sequence of peptide fragment M3 is SEQ ID NO.3：Val-Ser-Leu-Trp-Pro-Cys-Arg.
Present invention also provides a kind of preparation methods of facial mask.
A kind of preparation method of facial mask as described in the present application, includes the following steps：
10-20 parts of cuttlefish polypeptide powders, 15-20 parts of moisturizer, 5-15 parts of Katsumadai seed extracts and 20-40 parts of water is taken to mix Facial mask liquid is made after conjunction, the facial mask liquid is coated on to the surface of the face paper, then will be enriched in the facial mask of facial mask liquid Paper is vacuum-packed, and obtains the facial mask, wherein, facial mask described in 50-200mg is coated on the face paper every square centimeter Liquid.
The preparation method of above-mentioned facial mask is simple, and based on zymolysis technique, reaction condition is mild, and the facial mask of preparation has anti-aging Effect, cuttlefish polypeptide powder and Katsumadai seed extract have good synergistic effect, can effectively remove interior free yl (O2-、 H2O2, ROO and-OH etc.), inoxidizability is high, and non-stimulated to skin, has good moisture-keeping efficacy.
The preparation method of the cuttlefish polypeptide powder includes the following steps in one of the embodiments,：
1) cuttlefish is chosen, is removed internal organs, and washes with water clean, homogenizer homogeneous meat mud；
2) it is 1 by mass volume ratio:3 to 1:5 solid-liquid ratio adds water to obtain mixed liquor, reconciles the pH value 7.0- of mixed liquor 8.0, protease hydrolyzed is added in, the additive amount of protease is that 500U-1500U is added in every gram of muddy flesh, and the protease is pancreas egg The mass ratio of white enzyme and papain, the trypsase and the papain is 1:3 to 1:5, then at 40-60 DEG C at enzymolysis 2-3h, enzyme deactivation are managed, centrifugation takes supernatant；
3) by the supernatant Ultra filtration membrane of 20000Da, and the ultrafiltrate below molecular weight 20000Da is taken, it is cold Dry obtained Gly-His-Lys A is lyophilized；
4) the Gly-His-Lys A is dissolved in by 20-25mg/mL in distilled water, and by Sephadex G-25 chromatography post separations Purifying, collects refined solution of the molecular weight between 400-1600Da, concentration, and freeze-drying obtains Gly-His-Lys B, then uses deionized water 1mg/mL solution is prepared, 0.22-0.30 μm of miillpore filter is crossed, is further isolated and purified with reverse phase liquid chromatography, then is sieved through activity Choosing obtains cuttlefish polypeptide powder after dry.
The elution requirement of the reverse phase liquid chromatography is in one of the embodiments,:The elution requirement of gradient elution is： 0.001-9min, water elution；9-40min, 0.001%-100% acetonitrile solution concentration by 0.001% linear increment extremely 100%；40-50min, acetonitrile elution.
The preparation method of the Katsumadai seed extract includes in one of the embodiments,：
Taking 80g-120g, seed extracts 3h in 80 DEG C with 70% methanol in one's early teens, and extract filtering, filter vacuum depressurizes dense Contracting, it is dry, obtain Katsumadai seed extract.
The drying is freeze-drying in one of the embodiments,.
Description of the drawings
Fig. 1 is the reversed efficient liquid phase C18 column elution profiles of cuttlefish polypeptide powder in the embodiment of the present invention 2.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with specific embodiment The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are only used to explain the present invention, It is not intended to limit the present invention.
Unless otherwise defined, all of technologies and scientific terms used here by the article is with belonging to technical field of the invention The normally understood meaning of technical staff is identical.Term used in the description of the invention herein is intended merely to description tool The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein " and/or " including one or more The arbitrary and all combination of relevant Listed Items.
Present invention also provides a kind of preparation methods of facial mask.
A kind of preparation method of facial mask as described in the present application, includes the following steps：
10-20 parts of cuttlefish polypeptide powders, 15-20 parts of moisturizer, 5-15 parts of Katsumadai seed extracts and 20-40 parts of water is taken to mix Facial mask liquid is made after conjunction, facial mask liquid is coated on to the surface of face paper, the face paper that then will be enriched in facial mask liquid carries out vacuum packet It fills to get facial mask, wherein, 50-200mg facial mask liquids are coated on face paper every square centimeter.
Wherein, the surface that facial mask liquid is coated on to face paper refers to the facial mask liquid slurry after mixing being applied to entire facial mask It on paper, is impregnated compared to face paper is placed in facial mask liquid, more facial mask liquids, the facial mask antioxidant effect of preparation can be adsorbed More preferably.Preferably, facial mask liquid is evenly applied to face paper surface, and gently pats, and facial mask liquid is made to penetrate into the fiber group of face paper In knitting.
50-200mg facial mask liquids are coated on face paper every square centimeter, can cause face paper reaches preferably to adsorb Amount.The facial mask liquid of extra 200mg is coated on face paper every square centimeter, during follow-up preservation, facial mask liquid can be precipitated, facial mask The deflection of paper declines.Coating is less than 50mg, the effect and unobvious of anti-aging on face paper every square centimeter.
In a preferred embodiment, the preparation method of cuttlefish polypeptide powder includes the following steps：
1) cuttlefish is chosen, is removed internal organs, and washes with water clean, homogenizer homogeneous meat mud.
In a preferred embodiment, cuttlefish is selected from graceful formula sepiella maindroni de Rochebrune, this kind of cuttlefish meat is fine and close, protein content Height, the polypeptide activity of fighting against senium obtained after enzymolysis are high.
In a preferred embodiment, homogenizer homogeneous speed is 3000rpm, and the muddy flesh uniformity blended is good, profit In enzymolysis, degree of hydrolysis height.
2) by 1:3-1:The solid-liquid ratio of 5 (m/v) adds water to obtain mixed liquor, reconciles the pH value 7.0-8.0 of mixed liquor, adds in egg White enzyme enzymolysis, the additive amount of protease is adds in 500U-1500U in every gram of muddy flesh, protease is trypsase and papain, pancreas The mass ratio of protease and papain is 1:3-1:5, the then enzymolysis processing 2-3h at 40-60 DEG C, enzyme deactivation, centrifugation takes supernatant Liquid.
In a preferred embodiment, the additive amount of protease is adds in 800U-1500U in every gram of muddy flesh, it is preferable that egg For the additive amount of white enzyme to add in 1000-1200U, such as 1000U, 1100U, 1200U in every gram of muddy flesh, enzymolysis degree of hydrolysis is high.
In a preferred embodiment, the mass ratio of trypsase and papain is 1:3.8.It is digested under the conditions of this, water Xie Du higher, obtained polypeptide activity of fighting against senium are also higher compared to other combinations.
In a preferred embodiment, enzyme deactivation enzyme deactivation work mode well known in the art, no longer excessively repeats herein.Make To be preferred, enzyme deactivation is to heat 10-15min at 90 DEG C -100 DEG C.Enzyme deactivation effect is more preferable.
3) by the supernatant Ultra filtration membrane of 20000Da, and the ultrafiltrate below molecular weight 20000Da is taken, freezing is dry Dry obtained Gly-His-Lys A.
The effect of ultrafiltration is to remove the excessive polypeptide of molecular weight and unhydrolysed protein, and present inventor has found, point Son measure excessive polypeptide in mouse antioxidase activity measures experiment with blank control group (polypeptide for not taking in the application) phase It is more not notable than difference, no activity of fighting against senium.
4) Gly-His-Lys A is dissolved in by 20-25mg/mL in distilled water, and pure by Sephadex G-25 chromatography post separations Change, collect refined solution of the molecular weight between 400-1600Da, concentration, freeze-drying obtains Gly-His-Lys B, then matched with deionized water 1mg/mL solution processed is crossed 0.22-0.30 μm of miillpore filter, is further isolated and purified with reverse phase liquid chromatography, then through screening active ingredients, Cuttlefish polypeptide powder is obtained after drying.
Wherein, the main function of chromatography is that the micromolecule polypeptide of specified molecular weight range is separated and is enriched with.
Present inventor has found that Sephadex G-25 are more suitable for small-molecular-weight peptide purification, applicable separating ranges For the peptide purification of 1000Da-20000Da, recovery rate higher.
Wherein, the main function of reverse phase liquid chromatography is that Gly-His-Lys B is further isolated and purified.In a preferred embodiment In, the chromatographic column of reverse phase liquid chromatography is C18 chromatographic columns.
In a preferred embodiment, the elution requirement reversely eluted is：0.001-9min, water elution；9-40min, 0.001%-100% acetonitrile solutions concentration is by 0.001% linear increment to 100%；40-50min, acetonitrile elution.
Wherein, activity of fighting against senium screening mainly by evaluate the antioxidant activity of SOD, GSH-Px and CAT filter out it is anti- The high peptide of oxidation activity, antioxidant activity is high, then activity of fighting against senium is good.
In a preferred embodiment, the preparation method of Katsumadai seed extract includes：
Taking 80g-120g, seed extracts 3h in 80 DEG C with 70% methanol in one's early teens, and extract filtering, filter vacuum depressurizes dense Contracting, it is dry, obtain Katsumadai seed extract.
Preferably, it is dry for freeze-drying.
The preparation method of above-mentioned facial mask is simple, and based on zymolysis technique, reaction condition is mild, and the facial mask of preparation has anti-aging Effect, cuttlefish polypeptide powder and Katsumadai seed extract have good synergistic effect, can effectively remove interior free yl (O2-、 H2O2, ROO and-OH etc.), inoxidizability is high, and non-stimulated to skin, has good moisture-keeping efficacy.
Below in conjunction with specific embodiment, the invention will be further elaborated.
Embodiment 1
Take 100g cuttlefishes polypeptide powder, 150g ceramides, 50g Katsumadai seed extracts and 200g water homogenizers Facial mask liquid is made after 3000rpm mixing, facial mask liquid is coated on to the surface of face paper, is coated on face paper every square centimeter 50mg facial mask liquids, the face paper that then will be enriched in facial mask liquid are vacuum-packed to get facial mask W1.
Wherein, the preparation method of cuttlefish polypeptide powder is：
1) cuttlefish is chosen, is removed internal organs, and washes with water clean, homogenizer homogeneous meat mud 1kg.
2) by 1:The solid-liquid ratio of 4 (m/v) adds water to obtain mixed liquor, reconciles the pH value 7.0 of mixed liquor, adds in protease enzyme Solution, the additive amount of protease are to add in 1000U in every gram of muddy flesh, wherein, the mass ratio of trypsase and the papain is 1: 3.8, the enzymolysis processing 2h at 45 DEG C, enzyme deactivation, centrifugation take supernatant.
3) by the supernatant Ultra filtration membrane of 20000Da, and the ultrafiltrate below molecular weight 20000Da is taken, freezing is dry Dry obtained Gly-His-Lys A1.
4) Gly-His-Lys A1 is dissolved in by 22mg/mL in distilled water, and column separating purification is chromatographed by Sephadex G-25, Refined solution of the molecular weight between 400-1600Da, concentration are collected, freeze-drying obtains Gly-His-Lys B1.Then it is prepared with deionized water 1mg/mL solution is crossed 0.22 μm of miillpore filter, is further isolated and purified with reverse phase liquid chromatography, the condition of chromatography is：It is purple Outer Detection wavelength setting 280nm, applied sample amount 2mL, elution speed 1ml/min, C18Hypersil BDS (250mm × 4.6mm, 5 μm), column temperature, 30 DEG C；Mobile phase is acetonitrile-aqueous solution, and the elution requirement of gradient elution is：0-9min, water elution； 9-40min, 0%-100% acetonitrile solution concentration are by 0% linear increment to 100%；40-50min, acetonitrile elution.Freezing is dry Cuttlefish polypeptide powder is obtained after dry.
The preparation method of Katsumadai seed extract is：
Taking 80g-120g, seed extracts 3h in 80 DEG C with 70% methanol in one's early teens, and extract filtering, filter vacuum depressurizes dense Contracting, freeze-drying, obtains Katsumadai seed extract.
Embodiment 2
Take 160g cuttlefishes polypeptide powder, 150g ceramides, 120g Katsumadai seed extracts and 200g water homogenizers Facial mask liquid is made after 3000rpm mixing, facial mask liquid is coated on to the surface of face paper, is coated on face paper every square centimeter 150mg facial mask liquids, the face paper that then will be enriched in facial mask liquid are vacuum-packed to get facial mask W2.
The preparation method is the same as that of Example 1 for cuttlefish polypeptide powder and Katsumadai seed extract.
Embodiment 3
Take 180g cuttlefishes polypeptide powder, 190g ceramides, 150g Katsumadai seed extracts and 400g water homogenizers Facial mask liquid is made after 3000rpm mixing, facial mask liquid is coated on to the surface of face paper, is coated on face paper every square centimeter 200mg facial mask liquids, the face paper that then will be enriched in facial mask liquid are vacuum-packed to get facial mask W3.
The preparation method is the same as that of Example 1 for cuttlefish polypeptide powder and Katsumadai seed extract.
Comparative example 1
Facial mask is made after taking 180g cuttlefishes polypeptide powder, 190g ceramides and 400g water homogenizers 3000rpm mixing Facial mask liquid is coated on the surface of face paper, coats 200mg facial mask liquids on face paper every square centimeter, then will be enriched in by liquid The face paper of facial mask liquid is vacuum-packed to get facial mask X1.
The preparation method is the same as that of Example 1 for cuttlefish polypeptide powder.
Comparative example 2
Face is made after taking 190g ceramides, 150g Katsumadai seed extracts and 400g water homogenizers 3000rpm mixing Facial mask liquid is coated on the surface of face paper, 200mg facial mask liquids is coated on face paper every square centimeter by film liquid, then will be rich Face paper containing facial mask liquid is vacuum-packed to get facial mask X2.
The preparation method is the same as that of Example 1 for Katsumadai seed extract.
Performance test：
1st, facial mask surface application qualification test
Example 1-3, comparative example 1, the facial mask of comparative example 2 and commercially available certain brand facial mask containing anti-oxidation efficacy are made respectively For test specimen.Selecting the people of several 40-50 Sui, application method is as volunteer：:After cleaning face, same size is taken Each test group and control group facial mask are pasted on face skin, are gently patted and are absorbed, each daily morning and evening to use once, every time 20-30min is sticked, it is perpendicular to hold using 2 months.It is given a mark respectively to disparity items according to change of skin, disparity items includes protecting Wet effect, wrinkle variation, skin elasticity, tightening effect, each project full marks are 100 points, and score value is higher, shows that effect is better. Marking is counted as a result, then averaging, obtains final appraisal result.Concrete outcome is shown in Table 1.
Table 1
As shown in Table 1, in the facial mask comprehensive score of the embodiment of the present application 1-3 each index be above it is commercially available containing anti-oxidation efficacy Certain brand facial mask, X1 facial masks (be free of Katsumadai seed extract) and X2 facial masks (without cuttlefish polypeptide powder).X1 facial masks are better than X2 again Facial mask, it is seen that cuttlefish polypeptide powder occupies leading position in facial mask liquid, plays the role of to anti-aging and moisturizing vital. In addition, as can be known from Table 1, the effect of W2 facial masks is best, further to visit to find out its cause, inventor has done following animal examination again It tests.
2nd, animal experiment
Using SOD, CAT, GSH-PX, MDA as the evaluation index of anti-aging, facial mask liquid in each embodiment is investigated respectively Anti-aging effects.
2.1 materials and reagent
SPF grades, 190 ± 20g of weight of SD rats, it is male female fifty-fifty.
Total number born (SOD) assay kit, malonaldehyde (MDA) assay kit, glutathione peroxidating Object enzyme (GSH-PX) assay kit (colorimetric method) is built up Bioengineering Research Institute by Nanjing and is provided.
2.2 animal models are built
Skin aging model is using D- gal Agings model and is aided with ultraviolet light.
D- gal Aging models
Each group rat skin lower injection 80mg/ (kg.d) D- galactolipins, all experimental animals are injected weekly twice, and continuous seven Week.Back hits exactly skin depilatory, the skin of exposure 2cm*2cm.Test group, which is given, smears facial mask liquid (4%w/ in embodiment 1-3 W), comparative example group gives the facial mask liquid (4%w/w) smeared in comparative example 1-2, control group gives smearing distilled water.All experiments Animal is smeared once daily, continuous seven weeks.
Ultraviolet light
With the UVB lamps of 80w to the irradiation at distance about 60cm of each group rat, primary at interval of irradiation in two days, each 1h connects It is seven weeks continuous.
After 7th week last day smeared, animal orbital venous plexus venous blood sampling, according to MDA, SOD, GSH-PX kit Illustrate to operate.
3rd, result
Table 2
Group SOD(U/mL) MDA(nmol/mL) GSH-PX(U.ml/min)
Control group 120.2±4.4 10.33±0.43 15.39±4.22
W1 163.4±1.2** 8.33±0.44* 17.33±3.88*
W2 174.2±3.3** 7.44±0.32** 20.12±2.11**
W3 156.2±1.9** 8.21±1.63* 16.42±1.33*
X1 149.3±6.9* 8.72±0.92* 15.93±3.39
X2 136.4±5.3 9.11±0.34 15.21±2.33
Note：* P ＜ 0.05 compared with the control group, compared with the control group * * P<0.01
From Table 2, it can be seen that for SOD, GSH-PX activity in W1, W2, W3 group in mouse body are compared with control group, measure It is worth higher, significant difference (P ＜ 0.05), W2 groups effect in These parameters evaluation is best, difference extremely significantly (P ＜ 0.01).This also coincide with facial mask surface application qualification test evaluation result.The facial mask liquid in facial mask liquid and X2 in X1 groups is although anti-ageing Old effect will be got well compared with control group.But still there is apparent gap compared with W1-W3 groups, and the anti-aging effects of X1 groups are better than X2 Group has also confirmed cuttlefish polypeptide powder from side and has played a major role, and Katsumadai seed extract plays synergistic effect.
In addition, the amino acid of the cuttlefish polypeptide powder in facial mask formula of liquid in embodiment 2 is sequenced, referring to Fig. 1, obtain Three sections of peptide section sequences are：
The amino acid sequence of peptide fragment M1 forms：Tyr-Leu-Ser-Trp-Lys-Leu
The amino acid sequence of peptide fragment M2 forms：Leu-Leu-Thr-Asp-Val-Tyr-Gln
The amino acid sequence of peptide fragment M3 forms：Val-Ser-Leu-Trp-Pro-Cys-Arg.
It should be noted that the parts by weight in each embodiment, i.e. mass parts, that is, mass fraction, Ke Yili Xie Weike, milligram, kilogram, jin, kilogram, pound, ton etc..By gram for, for example, 1 parts by weight is in 0.0001 to 10000 grams A certain quality；For example, 1 parts by weight can be 0.001g, 0.01g, 0.02g, 0.05g, 0.1g, 0.2g, 0.5g, 1g, 2g, 3g, 4g, 5g, 10g, 15g, 20g, 30g, 50g, 80g, 100g, 500g, 1000g, 5000g, 10000g or 50000g etc., and be not limited to This, manufactures according to actual production and selects, each embodiment and so on.For example, 1 parts by weight are 1 gram, 0.1 parts by weight are 0.1 gram, and so on.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that those of ordinary skill in the art are come It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention Range.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Sequence table
<110>Guangzhou Sai Laila stem cells Science and Technology Co., Ltd.
<120>Facial mask and preparation method thereof
<141> 2018-02-02
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 6
<212> PRT
<213> Artificial Sequence
<400> 1
Thr Leu Ser Thr Leu Leu
1 5
<210> 2
<211> 7
<212> PRT
<213> Artificial Sequence
<400> 2
Leu Leu Thr Ala Val Thr Gly
1 5
<210> 3
<211> 7
<212> PRT
<213> Artificial Sequence
<400> 3
Val Ser Leu Thr Pro Cys Ala
1 5

Claims (10)

1. a kind of facial mask, which is characterized in that the facial mask includes face paper and facial mask liquid, by weight, the facial mask liquid packet Following raw materials are included to be made：
10-20 parts of cuttlefish polypeptide powders；
15-20 parts of moisturizer；
5-15 parts of Katsumadai seed extracts；
And 20-40 parts of water.
2. facial mask according to claim 1, which is characterized in that the face paper is silk mask cloth or alginate fibre.
3. facial mask according to claim 1, which is characterized in that the moisturizer is ceramide.
4. facial mask according to claim 1, which is characterized in that the molecular weight of the cuttlefish polypeptide powder is 400Da- 1600Da。
5. facial mask according to claim 4, which is characterized in that the cuttlefish polypeptide powder includes following several peptide fragments：
The amino acid sequence of peptide fragment M1 is SEQ ID NO.1：Tyr-Leu-Ser-Trp-Lys-Leu；
The amino acid sequence of peptide fragment M2 is SEQ ID NO.2：Leu-Leu-Thr-Asp-Val-Tyr-Gln；
The amino acid sequence of peptide fragment M3 is SEQ ID NO.3：Val-Ser-Leu-Trp-Pro-Cys-Arg.
6. a kind of preparation method of facial mask as described in claim 1, which is characterized in that include the following steps：
After taking 10-20 parts of cuttlefish polypeptide powders, 15-20 parts of moisturizer, 5-15 parts of Katsumadai seed extracts and 20-40 parts of water mixing Facial mask liquid is made, the facial mask liquid is coated on to the surface of the face paper, then will be enriched in the face paper of facial mask liquid into Row vacuum packaging, obtains the facial mask, wherein, facial mask liquid described in 50-200mg is coated on the face paper every square centimeter.
7. the preparation method of facial mask according to claim 6, which is characterized in that the preparation method of the cuttlefish polypeptide powder includes Following steps：
1) cuttlefish is chosen, is removed internal organs, and washes with water clean, homogenizer homogeneous meat mud；
2) it is 1 by mass volume ratio:3 to 1:5 solid-liquid ratio adds water to obtain mixed liquor, reconciles the pH value 7.0-8.0 of mixed liquor, adds Enter protease hydrolyzed, the additive amount of protease to add in 500U-1500U in every gram of muddy flesh, the protease for trypsase with The mass ratio of papain, the trypsase and the papain is 1:3 to 1:5, the then enzymolysis processing 2- at 40-60 DEG C 3h, enzyme deactivation, centrifugation take supernatant；
3) by the supernatant Ultra filtration membrane of 20000Da, and the ultrafiltrate below molecular weight 20000Da is taken, freezing is dry Dry obtained Gly-His-Lys A；
4) the Gly-His-Lys A is dissolved in by 20-25mg/mL in distilled water, and pure by Sephadex G-25 chromatography post separations Change, collect refined solution of the molecular weight between 400-1600Da, concentration, freeze-drying obtains Gly-His-Lys B, then matched with deionized water 1mg/mL solution processed is crossed 0.22-0.30 μm of miillpore filter, is further isolated and purified with reverse phase liquid chromatography, then through screening active ingredients, Cuttlefish polypeptide powder is obtained after drying.
8. the preparation method of facial mask according to claim 7, which is characterized in that the elution requirement of the reverse phase liquid chromatography For:The elution requirement of gradient elution is：0.001-9min, water elution；9-40min, 0.001%-100% acetonitrile solution concentration By 0.001% linear increment to 100%；40-50min, acetonitrile elution.
9. the preparation method of facial mask according to claim 6, which is characterized in that the preparation method packet of the Katsumadai seed extract It includes：
Taking 80g-120g, seed extracts 3h, extract filtering in 80 DEG C with 70% methanol in one's early teens, and filter vacuum is concentrated under reduced pressure, does It is dry, obtain Katsumadai seed extract.
10. the preparation method of facial mask according to claim 9, which is characterized in that the drying is freeze-drying.
CN201810108057.1A 2018-02-02 2018-02-02 facial mask and preparation method thereof Withdrawn CN108210416A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810108057.1A CN108210416A (en) 2018-02-02 2018-02-02 facial mask and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810108057.1A CN108210416A (en) 2018-02-02 2018-02-02 facial mask and preparation method thereof

Publications (1)

Publication Number Publication Date
CN108210416A true CN108210416A (en) 2018-06-29

Family

ID=62669992

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201810108057.1A Withdrawn CN108210416A (en) 2018-02-02 2018-02-02 facial mask and preparation method thereof

Country Status (1)

Country Link
CN (1) CN108210416A (en)

Similar Documents

Publication Publication Date Title
US11001607B2 (en) Peptide, composition comprising said peptide and uses thereof, in particular cosmetic uses
EP3432991B1 (en) Cosmetic use of an ingredient derived from marrubium vulgare
EP2760429B1 (en) New cosmetic use of an albizia julibrissin extract and corresponding topical composition
KR102578792B1 (en) Topical cosmetic treatment for skin and scalp based on celery extract and corresponding active ingredients
EP3746041A1 (en) Use of cyclic peptides in cosmetic
EP3244971A1 (en) Use of plant cells of leontopodium alpinum for a cosmetic treatment and corresponding active ingredient
WO2016097966A1 (en) Tripeptides, compositions thereof and their cosmetic uses
CN111732634B (en) Application of earthworm active peptide for inhibiting melanocyte proliferation in preparation of medicines or cosmetics
CN108210416A (en) facial mask and preparation method thereof
WO2020165365A1 (en) Active ingredient for homogenizing complexion, in particular skins with an olive carnation
CN111770778B (en) Extract of Pelargonium gracilis rich in organic acid, cosmetic composition containing the same and cosmetic use thereof
KR102529252B1 (en) Cosmetic composition with a low molecular collagen peptides from Hydrangea Macrophylla
WO2022023406A1 (en) Cosmetic or dermatological peptide-based treatment of the skin and its integuments
CN108309853A (en) Anti-ageing face mask and preparation method thereof
CN112040968B (en) Bioactive peptides with high binding affinity to human muscle nicotinic acetylcholine receptors
CN108191959A (en) Anti-oxidation peptide and preparation method thereof and the cosmetics comprising the anti-oxidation peptide
CN108059662A (en) Anti-oxidation peptide and preparation method thereof and the cosmetics comprising the anti-oxidation peptide
KR20180047207A (en) Cosmetic composition for skin regeneration and moisturing containing beta-glucan and anti-aging peptide
US20230398049A1 (en) Tetrapeptides, compositions comprising them, and their cosmetic use
EP4037656A1 (en) Use of leontodium alpinum plant cells for an anti-glycation anti-ageing skin treatment
WO2021185953A1 (en) Cosmetic composition and treatment for treating skin sagging
EP3518888A1 (en) Alkyl-phtalides for use in the cosmetic treatment of nails
AU2002305718A1 (en) Composition containing a peptide and a pigment and the use thereof in darkening the skin

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20180629