CN108205045A - The detection method of protein content in a kind of azlon - Google Patents
The detection method of protein content in a kind of azlon Download PDFInfo
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- CN108205045A CN108205045A CN201711334889.7A CN201711334889A CN108205045A CN 108205045 A CN108205045 A CN 108205045A CN 201711334889 A CN201711334889 A CN 201711334889A CN 108205045 A CN108205045 A CN 108205045A
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- protein
- azlon
- detection method
- protein content
- fiber
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- Artificial Filaments (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Abstract
The present invention discloses a kind of detection method of protein content in azlon.Weigh a certain amount of azlon to dry to constant weight, it is put into digest tube, add in a certain amount of copper sulphate, potassium sulfate and the concentrated sulfuric acid, then a period of time is digested in the digesting of steady temperature, it is tested on Protein Analyzer after cooling, converts to obtain the content of protein by the protein conversion factor of setting.The method overcome the shortcomings of previous Kjeldahl's method and sodium-hypochlorite process are complicated for operation, the test period is long, have many advantages, such as that the test period is short, applied widely, efficient, easy to operate, result is reproducible.
Description
Technical field
The present invention relates to a kind of detection method of textile component, protein contains in especially a kind of azlon
The detection method of amount.
Background technology
Regenerated protein is that protein solution is extracted from animal or plant, and physical blending is carried out with other high polymers
Or chemical copolymerization, a kind of Neu tuberosum obtained by wet spinning have soybean protein to answer using comparative maturity at present
Condensating fiber, protein cellulose viscose and milk protein fiber.Due to containing protein component in regenerated protein, have good
Good skin-friendly and comfort.
Protein content has an impact the performance of regenerated protein, is a weight for evaluating azlon performance
Want index.Chinese patent CN104020075A discloses a kind of method for quantitatively detecting protein content in protein cellulose viscose,
It dries to constant weight protein cellulose viscose, and cooling is moved into after weighing in tool plug conical flask, adds in a concentration of 0.30mol/ of 100mL
The liquor natrii hypochloritis of L vibrates 30min in 25 DEG C of water-bath, and then residue is transferred in glass sand core crucible, washing
Residue is dried to constant weight in an oven afterwards, the mathematic interpolation for dissolving front and rear weight through liquor natrii hypochloritis according to fiber goes out albumen
Matter content.Have before and after this method and fiber dries to constant weight twice, each drying time is generally no less than 4h, and the test period is long.It should
It is also referred in patent using the Kjeldahl's method for determining nitrogen distilling apparatus, this method test period is longer, in addition to drying fibrous
Outside, it is also necessary to digest, the problem of due to device, one sample of digestion just needs 3 ~ 4h even longer times, and set of device
Primary to be only capable of one sample of digestion, efficiency is very low.And both methods is only used for protein cellulose viscose, the scope of application is small.
Invention content
The technical problems to be solved by the invention are：For the deficiency of current detection method, provide a kind of test period it is short,
The detection method of protein content in applied widely, efficient azlon.In order to solve the above technical problems, this
The technical solution of invention is：
The detection method of protein content, includes the following steps in a kind of azlon：
（1）When azlon is long filament, long filament is cut into the long segments of 10mm or so, measuring cup is put into, works as regeneration
When protein fibre is staple fiber, fiber is directly put into measuring cup, then measuring cup exists（105±3）DEG C drying box in
It dries to constant weight, covers bottle cap, move into the drier equipped with discoloration silica gel, cooling.
（2）Weigh fiber 0.2g ~ 0.8g after cooling rapidly, be put into digest tube, then into digest tube add in 0.2g ~
Digest tube is placed in 400 DEG C ~ 440 DEG C of porous digesting by 0.4g copper sulphate, 6g ~ 10g potassium sulfates and 12mL ~ 20mL concentrated sulfuric acids
In, 45min ~ 75min is digested, room temperature cools down after taking-up.
（3）Protein Analyzer Parameter Conditions are set, and prepare enough sodium hydroxide solutions, hydrochloric acid or sulfuric acid standard
Solution and the boric acid solution containing mixed indicator.Protein Analyzer Parameter Conditions are set as：Dilution（Deionization
Water）Addition 30mL ~ 50mL, acceptable solution（Contain the boric acid solution that mixed indicator mass fraction is 1% ~ 4%）Addition 30mL ~
Sodium hydroxide solution addition 50mL ~ 80mL of 50mL, a concentration of 400g/L, distillation time 5min ~ 10min, protein conversion
Coefficient is 6.25.
（4）Digest tube after cooling on Protein Analyzer is tested, system is converted by the protein of setting
The automatic conversion of number obtains protein content.
The azlon includes composite fiber with soybean protein, protein cellulose viscose etc..
The hydrochloric acid or sulfuric acid concentration of standard solution are 0.05mol/L ~ 0.1mol/L.
The present invention has the following advantages：
1st, it is applied widely.The azlons such as composite fiber with soybean protein, protein cellulose viscose are applicable in.
2nd, the test period is short.Especially shorten digestion time, longest 75min, distillation time longest 10min, instrument is straight
Tap into capable titration.
3rd, it is efficient.Porous digesting can once digest 8 even more samples according to the number in hole.
4th, step is simple, easy to operate, as a result reproducible.
Specific embodiment
With reference to specific embodiment, the present invention is further explained.
Embodiment 1
The soybean protein composite short fiber of sufficient amount is put into measuring cup,（105±3）DEG C drying box in dry to constant weight, cover
Good bottle cap moves into measuring cup in the drier equipped with discoloration silica gel, cooling.
Weigh fiber 0.2g after cooling rapidly, be put into digest tube, then added in into digest tube 0.4g copper sulphate,
10g potassium sulfates and the 12mL concentrated sulfuric acids, digest tube is placed in 420 DEG C of porous digesting, digests 45min, room temperature is cold after taking-up
But.
Protein Analyzer Parameter Conditions are set：Dilution（Deionized water）Addition 50mL, acceptable solution（Contain mixing
Indicator mass fraction is 4% boric acid solution）The sodium hydroxide solution addition 50mL of addition 50mL, a concentration of 400g/L,
Distillation time 9min, protein conversion factor are 6.25.A concentration of 0.1118mol/L of hydrochloric acid standard solution
Digest tube after cooling is tested on Protein Analyzer, the results are shown in Table 1 for gained protein content.
Protein content in 1 composite fiber with soybean protein of table
| Serial number | Protein content/% |
| 1 | 18.51 |
| 2 | 18.84 |
| 3 | 19.02 |
| 4 | 18.53 |
| 5 | 19.44 |
| 6 | 19.18 |
| 7 | 19.24 |
| 8 | 19.12 |
| 9 | 18.64 |
| 10 | 18.71 |
| Average value | 18.92 |
| Standard deviation | 0.32 |
Embodiment 2
The pupa albumen viscose staple fibre of sufficient amount is put into measuring cup,（105±3）DEG C drying box in dry to constant weight, cover
Good bottle cap moves into measuring cup in the drier equipped with discoloration silica gel, cooling.
Weigh fiber 0.2g after cooling rapidly, be put into digest tube, then added in into digest tube 0.4g copper sulphate,
10g potassium sulfates and the 12mL concentrated sulfuric acids, digest tube is placed in 420 DEG C of porous digesting, digests 45min, room temperature is cold after taking-up
But.
Protein Analyzer Parameter Conditions are set：Dilution（Deionized water）Addition 50mL, acceptable solution（Contain mixing
Indicator mass fraction is 4% boric acid solution）The sodium hydroxide solution addition 50mL of addition 50mL, a concentration of 400g/L,
Distillation time 9min, protein conversion factor are 6.25.A concentration of 0.1118mol/L of hydrochloric acid standard solution
Digest tube after cooling is tested on Protein Analyzer, the results are shown in Table 2 for gained protein content.
Protein content in 2 pupa albumen viscose rayon of table
| Serial number | Protein content/% |
| 1 | 2.62 |
| 2 | 2.48 |
| 3 | 2.49 |
| 4 | 2.40 |
| 5 | 2.34 |
| 6 | 2.44 |
| 7 | 2.23 |
| 8 | 2.45 |
| 9 | 2.38 |
| 10 | 2.38 |
| Average value | 2.42 |
| Standard deviation | 0.10 |
Claims (4)
1. the detection method of protein content in a kind of azlon, it is characterised in that include the following steps：
（1）When azlon is long filament, long filament is cut into the long segments of 10mm or so, measuring cup is put into, works as regeneration
When protein fibre is staple fiber, fiber is directly put into measuring cup, then measuring cup exists（105±3）DEG C drying box in
It dries to constant weight, covers bottle cap, move into the drier equipped with discoloration silica gel, cooling；
（2）Fiber 0.2g ~ 0.8g after cooling is weighed rapidly, is put into digest tube, and 0.2g ~ 0.4g is then added in into digest tube
Digest tube is placed in 400 DEG C ~ 440 DEG C of porous digesting, disappears by copper sulphate, 6g ~ 10g potassium sulfates and 12mL ~ 20mL concentrated sulfuric acids
Change 45min ~ 75min, room temperature cools down after taking-up；
（3）Protein Analyzer Parameter Conditions are set, and prepare enough sodium hydroxide solutions, hydrochloric acid or sulfuric acid standard solution
And the boric acid solution containing mixed indicator；
（4）Digest tube after cooling is tested on Protein Analyzer, by the protein conversion factor of setting from
Dynamic conversion obtains protein content.
2. the detection method of protein content in the azlon according to claims 1, it is characterised in that：Again
Raw egg white matter fiber is composite fiber with soybean protein, protein cellulose viscose etc..
3. the detection method of protein content in the azlon according to claims 1, it is characterised in that：From
Dynamic kjeldahl apparatus Parameter Conditions are set as：Dilution（Deionized water）Addition 30mL ~ 50mL, acceptable solution（Refer to containing mixing
Show the boric acid solution that agent mass fraction is 1% ~ 4%）The sodium hydroxide solution of addition 30mL ~ 50mL, a concentration of 400g/L add in
50mL ~ 80mL, distillation time 5min ~ 10min are measured, protein conversion factor is 6.25.
4. according to the detection method of protein content in the azlon described in claims 1, it is characterised in that：Hydrochloric acid
Or sulfuric acid concentration of standard solution is the mol/L of 0.05mol/L ~ 0.1.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108802264A (en) * | 2018-07-09 | 2018-11-13 | 天津华勘商品检验有限公司 | A kind of kjeldahl apparatus measures the detection method of nitrogen in carburant |
| CN110082510A (en) * | 2019-05-08 | 2019-08-02 | 江苏科技大学 | A method of identifying mulberry silk and ricinus silk |
| CN114813718A (en) * | 2022-04-13 | 2022-07-29 | 宝鸡市质量技术检验检测中心(宝鸡市质量技术检验检测研究院)(宝鸡市纤维质量监测中心) | Method for detecting content of regenerated protein fiber protein |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294914A (en) * | 2007-04-27 | 2008-10-29 | 李立华 | Computer full-automatic Kjeldahl determination device |
| CN104020075A (en) * | 2014-06-19 | 2014-09-03 | 上海市纺织科学研究院 | Method for quantitatively detecting content of protein in protein viscose |
| CN104122367A (en) * | 2014-08-12 | 2014-10-29 | 山东百盛生物科技有限公司 | Technology for rapidly detecting content of liquid starch protein |
| CN104198646A (en) * | 2014-08-15 | 2014-12-10 | 广州衡创测试技术服务有限公司 | Improved protein determination method |
| CN105784920A (en) * | 2016-04-14 | 2016-07-20 | 梧州市产品质量检验所 | Feed protein detection method |
| CN106442848A (en) * | 2016-10-19 | 2017-02-22 | 中国农业科学院烟草研究所 | Method for testing protein in tobacco and tobacco products through automatic azotometer |
| CN107255624A (en) * | 2017-05-12 | 2017-10-17 | 华南农业大学 | A kind of fast and convenient method for determining protein content in fan's power mushroom entity |
-
2017
- 2017-12-14 CN CN201711334889.7A patent/CN108205045A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294914A (en) * | 2007-04-27 | 2008-10-29 | 李立华 | Computer full-automatic Kjeldahl determination device |
| CN104020075A (en) * | 2014-06-19 | 2014-09-03 | 上海市纺织科学研究院 | Method for quantitatively detecting content of protein in protein viscose |
| CN104122367A (en) * | 2014-08-12 | 2014-10-29 | 山东百盛生物科技有限公司 | Technology for rapidly detecting content of liquid starch protein |
| CN104198646A (en) * | 2014-08-15 | 2014-12-10 | 广州衡创测试技术服务有限公司 | Improved protein determination method |
| CN105784920A (en) * | 2016-04-14 | 2016-07-20 | 梧州市产品质量检验所 | Feed protein detection method |
| CN106442848A (en) * | 2016-10-19 | 2017-02-22 | 中国农业科学院烟草研究所 | Method for testing protein in tobacco and tobacco products through automatic azotometer |
| CN107255624A (en) * | 2017-05-12 | 2017-10-17 | 华南农业大学 | A kind of fast and convenient method for determining protein content in fan's power mushroom entity |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108802264A (en) * | 2018-07-09 | 2018-11-13 | 天津华勘商品检验有限公司 | A kind of kjeldahl apparatus measures the detection method of nitrogen in carburant |
| CN110082510A (en) * | 2019-05-08 | 2019-08-02 | 江苏科技大学 | A method of identifying mulberry silk and ricinus silk |
| CN114813718A (en) * | 2022-04-13 | 2022-07-29 | 宝鸡市质量技术检验检测中心(宝鸡市质量技术检验检测研究院)(宝鸡市纤维质量监测中心) | Method for detecting content of regenerated protein fiber protein |
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Application publication date: 20180626 |