CN108195984A - Multivitamin detection method and detecting system in a kind of dry blood cake - Google Patents
Multivitamin detection method and detecting system in a kind of dry blood cake Download PDFInfo
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- CN108195984A CN108195984A CN201711481553.3A CN201711481553A CN108195984A CN 108195984 A CN108195984 A CN 108195984A CN 201711481553 A CN201711481553 A CN 201711481553A CN 108195984 A CN108195984 A CN 108195984A
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- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention belongs to multivitamin detection method and detecting systems in the technical field of blood testing more particularly to a kind of dry blood cake.The present invention provides multivitamin detection methods in a kind of dry blood cake, include the following steps：S101：Internal standard compound, dry blood cake after being handled are added in dry blood cake；S102：Blood cake dry after processing with the first extract liquor is extracted, obtain the first detection liquid or extracts blood cake dry after processing with the second extract liquor, obtains the second detection liquid；S103：The described first detection liquid or the second detection liquid are detected using liquid chromatography mass combined instrument；Wherein, first extract liquor is the methanol aqueous solution that percent by volume is 5% 95%；Second extract liquor is 10 90% methanol aqueous solution.Multivitamin in the detection method energy short time of the present invention in detection micro blood, solution detect technological deficiency time-consuming and laborious in multivitamin method at present.
Description
Technical field
The invention belongs to multivitamin detection methods in the technical field of blood testing more particularly to a kind of dry blood cake
And detecting system.
Background technology
The vniims method of currently used detection human body is detected using blood plasma, and plasma sample is passed through sodium hydroxide alkali
Upper machine is measured after change, protein precipitation and petroleum ether extraction concentration, however, what the vitamin detection method of the prior art needed
Blood is more, and blood preseration transporter noodles part is harsh, and can only detect single vitamin content every time, a variety of for detecting
During vitamin, the detection method of the prior art is very time-consuming and laborious.
Invention content
In view of this, the present invention provides multivitamin detection method and detecting system in a kind of dry blood cake, can have
Effect solves to detect technological deficiency time-consuming and laborious in multivitamin method at present.
The present invention also provides multivitamin detection methods in a kind of dry blood cake, include the following steps：
S101：Internal standard compound, dry blood cake after being handled are added in dry blood cake；
S102：Blood cake dry after processing is extracted with the first extract liquor, dry blood after obtaining the first detection liquid or handling
Spot is extracted with the second extract liquor, obtains the second detection liquid；
S103：The described first detection liquid or the second detection liquid are detected using liquid chromatograph-mass spectrometer；
Wherein, first extract liquor is the methanol aqueous solution that percent by volume is 5%-95%；Second extract liquor
Methanol aqueous solution for 10-90%.
More preferably, first extract liquor is the methanol aqueous solution that percent by volume is 70%；Second extract liquor
For 20% methanol aqueous solution
It should be noted that the first extract liquor is used to extract fat-soluble vitamin；Second extract liquor is water-soluble for extracting
The vitamin of property.
Preferably, the internal standard compound is specially Isotopic Internal Standard liquid, the Isotopic Internal Standard liquid 5-40 μ L.
More preferably, the 5 μ L of Isotopic Internal Standard liquid.
Preferably, the internal standard compound is thiamines-(- 5 base -13C3 of 4- methyl-1 3C- thiazoles) hydrochloride, riboflavin
(13C4,99%；15N2,98%), niacinamide -2,4,5,6-d4,25- hydroxyl ergosterols d3 (6,19,19-D3,97%), dimension life
Plain B5 (two-Beta-alanine -13C6,15N2) calcium salt, alpha-tocopherol-(ring -5,7- dimethyl-d6), pyridoxal/vitamin B6
It is one or more in (vitamin B6) (methyl D 3,98%) and alltrans retinol d5.
More preferably, thiamines-(4- methyl-1 3C- thiazole -5- bases -13C3) hydrochloride uses a concentration of 100ng/ml, core
Flavine (13C4,99%；15N2,98%) a concentration of 20ng/ml, niacinamide -2,4 are used, 5,6-d4 use a concentration of 1000ng/
Ml, 25- hydroxyl ergosterol d3 (6,19,19-D3,97%) using a concentration of 20ng/ml, vitamin B5 (two-Beta-alanine-
13C6,15N2) calcium salt using a concentration of 200ng/ml, alpha-tocopherol-(ring -5,7- dimethyl-d6) using a concentration of
10000ng/ml, pyridoxal/vitamin B6 (vitamin B6) (methyl D 3,98%) are regarded using a concentration of 100ng/ml, alltrans
Flavol d5 uses a concentration of 1000ng/ml.
Preferably, first extract liquor is 5-100 μ L, the extraction flow velocity of first extract liquor is 10-150 μ L/
ml。
More preferably, first extract liquor is 20 μ L, the extraction flow velocity of first extract liquor is 50 μ L/ml.
Preferably, second extract liquor is 5-100 μ L, the extraction flow velocity of second extract liquor is 10-150 μ L/
ml。
More preferably, second extract liquor is 20 μ L, the extraction flow velocity of second extract liquor is 50 μ L/ml.
Preferably, the S101 is further included：Processing, drying time 1-60s is dried in blood cake dry after processing.
More preferably, the drying time is 5s.
The present invention also provides multivitamin automatic checkout system in a kind of dry blood cake, including：Pretreatment unit, extraction
Take unit and detection unit；
The pretreatment unit is used to add in internal standard compound, dry blood cake after being handled to dry blood cake；
The extraction cells are extracted for dry blood cake after handling with the first extract liquor, obtain the first detection liquid or
Blood cake dry after processing with the second extract liquor is extracted, obtains the second detection liquid；
The detection unit to described first detection liquid or it is described second detection liquid using liquid chromatograph-mass spectrometer into
Row detection.
Preferably, it further includes：Cleaning unit is used to clean the extraction cells.
Preferably, when the extraction cells are extracted using the first extract liquor, cleaning unit is specially to the extraction
The outlet of unit is taken to carry out wash cycles using the first extract liquor, cleaning solution and the first extract liquor, the wash cycles program is：
S201：The outlet of the extraction cells is cleaned using first extract liquor, to clean for the first time；
S202：The outlet of extraction cells after being cleaned to first time is cleaned using the cleaning solution, is cleaned for second；
S203：The outlet of extraction cells after being cleaned to second is cleaned using first extract liquor, clear for third time
It washes；
The import of the extraction cells carries out single cleaning using the first extract liquor.
More preferably, the scavenging period of S201 cleanings for the first time is 5-200s.
More preferably, the scavenging period of S201 cleanings for the first time is 30s.
More preferably, second of scavenging period cleaned of the S202 is 5-200s.
More preferably, second of scavenging period cleaned of the S202 is 30s.
More preferably, the scavenging period of the S203 third times cleaning is 5-200s.
More preferably, the scavenging period of the S203 third times cleaning is 30s.
More preferably, the scavenging period of the first extract liquor single cleaning is 5-200s.
More preferably, the scavenging period of the first extract liquor single cleaning is 20s.
Preferably, when the extraction cells are extracted using the second extract liquor, cleaning unit is specially to the extraction
The outlet of unit is taken to carry out wash cycles using the second extract liquor, cleaning solution and the second extract liquor, the wash cycles program is：
S301：The outlet of the extraction cells is cleaned using second extract liquor, to clean for the first time；
S302：The outlet of extraction cells after being cleaned to first time is cleaned using the cleaning solution, is cleaned for second；
S303：The outlet of extraction cells after being cleaned to second is cleaned using second extract liquor, clear for third time
It washes；
The import of the extraction cells carries out single cleaning using the second extract liquor.
More preferably, the scavenging period of S301 cleanings for the first time is 5-200s.
More preferably, the scavenging period of S301 cleanings for the first time is 30s.
More preferably, second of scavenging period cleaned of the S302 is 5-200s.
More preferably, second of scavenging period cleaned of the S302 is 30s.
More preferably, the scavenging period of the S303 third times cleaning is 5-200s.
More preferably, the scavenging period of the S303 third times cleaning is 30s.
More preferably, the scavenging period of the single cleaning of second extract liquor is 5-200s.
More preferably, the scavenging period of the single cleaning of second extract liquor is 20s.
Preferably, the cleaning solution is specially acetone：Isopropanol：Methanol：The mixed liquor of water, wherein, the acetone：Institute
State isopropanol：The methanol：The volume ratio of the water is 1:1:1:1.
Dry blood cake (driedblood spots, DBS) sampling method, earliest by Robert Guthrie 1963 propose,
The positions such as finger tip, ear-lobe or heel are pricked by using disposable blood taking needle, drop in pipette, extract blood or directly filter
The method sampled on paper.Compared to conventional biological sample collection mode, dry blood cake method of sampling wound is small, passes through foot more
With, toe, finger take blood, sampling is very convenient.The present invention develops multivitamin detection method in a kind of dry blood cake, from
Multivitamin is accurately detected in the micro blood of dry blood cake.The detection method very simple of the present invention and quick, including S101：
Internal standard compound, dry blood cake after being handled are added in dry blood cake；S102：Blood cake dry after processing is extracted with the first extract liquor,
It obtains the first detection liquid or extracts blood cake dry after processing with the second extract liquor, obtain the second detection liquid；S103：Using
Liquid chromatograph-mass spectrometer is detected the first detection liquid or the second detection liquid；Wherein, first extract liquor is volume
Percentage is the methanol aqueous solution of 5%-95%；Methanol aqueous solution of second extract liquor for 10%-90%, the present invention first
Extract liquor and the second extraction liquid energy extract multivitamin from micro blood, and three steps will detect a variety of in dry blood cake
Vitamin and its testing result correlation and repeatability height, meanwhile, the present invention also provides a kind of automatic checkout system to dry blood
Spot realizes full-automatic extraction, avoids the possibility for occurring mistake in artificial extraction process, greatly improves weighing for extraction efficiency
Renaturation, moreover it is possible to realize the high throughput analysis of sample.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described.
Fig. 1 shows vitamin A standard curve；
Fig. 2 shows vitamin D standard curve；
Fig. 3 shows vitamin E standard curve；
Fig. 4 shows vitamin B1 standard curve；
Fig. 5 shows vitamin B2 standard curve；
Fig. 6 shows vitamin B3 standard curve；
Fig. 7 shows vitamin B5 standard curve；
Fig. 8 shows vitamin B6 standard curve；
Fig. 9 shows Vitamin B9 standard curve；
Figure 10 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin A；
Figure 11 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin D；
Figure 12 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin E；
Figure 13 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin B1；
Figure 14 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin B2；
Figure 15 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin B3；
Figure 16 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin B5；
Figure 17 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of vitamin B6；
Figure 18 shows dry blood cake detection method using the present invention and the inspection using conventional new blood vitamin detection method
Survey the result correlation analysis of Vitamin B9.
Specific embodiment
The present invention provides multivitamin detection method and detecting system in a kind of dry blood cake, the present invention can be examined quickly
The multivitamin in human body is surveyed, effectively solves existing time-consuming and laborious technological deficiency during detection multivitamin at present.
The technical solution in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's all other embodiments obtained without making creative work belong to the model that the present invention protects
It encloses.
Wherein, the raw material of following embodiment is commercially available or self-control.
It should be noted that the correspondence of targeted vitamins and target compound is as shown in Table 1 and Table 2.
1 fat-soluble targeted vitamins of table and target compound
| Targeted vitamins | Target compound |
| Vitamin A | Retinol (reitnol) |
| Vitamin D | Dihydroxy calciferol (2,5-hydroxycholcalciferol) |
| Vitamin E | Alpha-tocopherol (alpha-tocopherol) |
The water-soluble targeted vitamins of table 2 and target compound
| Targeted vitamins | Target compound |
| Vitamin B1 | Diphosphothiamine (thiamine diphosphate) |
| Vitamin B2 | Riboflavin (riboflavin) |
| Vitamin B3 | Niacinamide (nicotinamide) |
| Vitamin B5 | Pantothenic acid (pantothenicacid) |
| Vitamin B6 | Pyridoxol (pyridoxine) |
| Vitamin B9 | Folic acid (folic acid) |
Japanese Shimadzu Nexera UHPLC Ultra Performance Liquid Chromatography instruments, American AB Sciex4500 triple four
The compound mass spectrograph of pole bar-linear ion hydrazine
Embodiment 1
The present embodiment is to detect liposoluble vitamin in dry blood cake using the detection method of the present invention, the specific steps are：
1st, dry blood cake card sample is prepared：By fresh blood drop on special dry blood cake card, the blood volume of new blood is
25 μ L, are protected from light dry 2h.
2nd, the dry blood cake of step 1 is transferred in pretreatment unit using software, to dry blood cake sprinkling 5 in pretreatment unit
μ L Isotopic Internal Standard liquid；After dry 5s, dry blood cake after being handled.
3rd, blood cake dry after processing is transferred in extraction cells using software, target in dry blood cake is extracted with 20 μ L extract liquors
Liposoluble vitamin.After extraction, cleaning unit prepares to extract next dry blood cake after automatically cleaning extraction cells.
Specifically cleaning process is：S201：The outlet of extraction cells is cleaned using the first extract liquor, to clean for the first time；S202：To
The outlet of extraction cells after primary cleaning is cleaned using cleaning solution, is cleaned for second；S203：Extraction after being cleaned to second
The outlet of unit is taken to be cleaned using the first extract liquor, is cleaned for third time；The import of extraction cells is carried out using the first extract liquor
Single cleans.Internal standard solution, extraction process and cleaning process parameter see the table below 3.
3 parameter list of table
4th, the first detection liquid (20 μ L contain target compound extract liquor in blood) of extraction cells is input by inspection by software
It surveys unit and carries out liquid chromatograph-mass spectrometer detection.Liquid chromatograph parameter setting sees below list lattice；Mass spectrometer ion source is joined
Number setting sees below list lattice；Target compound MRM parameter settings see below list lattice.
4 parameter list of table
5 parameter list of table
Table 6
5th, interpretation of result：Liposoluble vitamin retention time, detection limit (LOD) and quantitative limit (LOQ such as tables 7.
7 parameter list of table
It please refers to Fig.1 to Fig.3, Fig. 1 to Fig. 3 (has marked regression equation and phase relation for liposoluble vitamin standard curve
Number R2)
According to diagram, the equal display target compound standard curve tool of related coefficient of vitamin A. D. E standard curve is preferable
It is linear.
Embodiment 2
The present embodiment is liposoluble vitamin stability test (RSD), and method is as follows：
6 dry blood cakes are made using same Fresh blood sample in experiment, and continuous extraction sample introduction is with this determining dry blood cake
The stability of vitamin detection method, the results are shown in Table 8, and as known from Table 8, the fat-soluble dimension life in dry blood cake is tested in this implementation
Element has good stability.
8 liposoluble vitamin stability data of table
| rep1 | rep2 | rep3 | rep4 | rep5 | rep6 | RSD (%) | |
| Vitamin A | 53543 | 59194 | 48479 | 51760 | 53715 | 54267 | 6.5 |
| Vitamin D | 65905 | 65251 | 60741 | 59389 | 67451 | 64248 | 4.9 |
| Vitamin E | 157640 | 166970 | 169310 | 189440 | 165480 | 193970 | 8.3 |
Embodiment 3
The present embodiment is to detect water soluble vitamin in dry blood cake using the detection method of the present invention, the specific steps are：
1st, dry blood cake card sample is prepared：By fresh blood drop on special dry blood cake card, the blood volume of new blood is
25 μ L, are protected from light dry 2h.
2nd, the dry blood cake of step 1 is transferred in pretreatment unit using software, to dry blood cake sprinkling 5 in pretreatment unit
μ L Isotopic Internal Standard liquid；After dry 5s, dry blood cake after being handled.
3rd, blood cake dry after processing is transferred in extraction cells using software, target in dry blood cake is extracted with 20 μ L extract liquors
Liposoluble vitamin.After extraction, cleaning unit prepares to extract next dry blood cake after automatically cleaning extraction cells.
Specifically cleaning process is：S301：The outlet of extraction cells is cleaned using the second extract liquor, to clean for the first time；S302：To
The outlet of extraction cells after primary cleaning is cleaned using cleaning solution, is cleaned for second；S303：Extraction after being cleaned to second
The outlet of unit is taken to be cleaned using the second extract liquor, is cleaned for third time；The import of extraction cells is carried out using the second extract liquor
Single cleans.Internal standard solution, extraction process and cleaning process parameter see the table below 8.
9 parameter list of table
4th, it is controlled by software the second detection liquid (20 μ L contain target compound extract liquor in blood) of extraction cells is defeated
Liquid chromatograph-mass spectrometer detection is carried out into detection unit.Liquid chromatograph parameter setting sees below list lattice；Mass spectrograph ion
Source parameter setting sees below list lattice；Target compound MRM parameter settings see below list lattice.
10 parameter list of table
11 parameter list of table
12 parameter list of table
5th, interpretation of result：(LOQ is as shown in table 12 for liposoluble vitamin retention time, detection limit (LOD) and quantitative limit.
13 liposoluble vitamin retention time of table, detection limit (LOD) and quantitative limit (LOQ)
Fig. 4 to Fig. 9 is please referred to, Fig. 4 to Fig. 9 (has marked regression equation and phase relation for water soluble vitamin standard curve
Number R2) vitamin B1, the related coefficient equal display target compound standard curve tool of B2, B3, B5, B6, B9 standard curve be preferable
It is linear.
Embodiment 4
The present embodiment is water soluble vitamin stability test (RSD), and method is as follows：
6 dry blood cakes are made using same Fresh blood sample in experiment, and continuous extraction sample introduction is with this determining dry blood cake
The stability of vitamin detection method, as a result as shown in table 13, as known from Table 13, the water soluble vitamin in dry blood cake is tested in this implementation
Raw element has good stability.
14 water soluble vitamin stability data of table
| rep1 | rep2 | rep3 | rep4 | rep5 | rep6 | RSD (%) | |
| Vitamin B1 | 4426 | 3957 | 4512 | 4466 | 4358 | 4679 | 5.5 |
| Vitamin B2 | 3799 | 4015 | 4198 | 4057 | 3658 | 3974 | 4.9 |
| Vitamin B3 | 31475000 | 34597000 | 32597000 | 36041000 | 34597000 | 32479000 | 5.1 |
| Vitamin B5 | 432510 | 478510 | 467910 | 459710 | 451020 | 461010 | 3.4 |
| Vitamin B6 | 1101500 | 1248900 | 1104400 | 1315000 | 1278100 | 1161400 | 7.6 |
| Vitamin B9 | 116552 | 117591 | 121798 | 102798 | 115467 | 120549 | 5.9 |
Embodiment 5
The present embodiment is dry blood cake detection method using the present invention with using conventional new blood vitamin detection method
Comparison embodiment, experimentation is：Using same Fresh blood sample, conventional manual is being used to extract and is being examined with liquid chromatography mass spectrometric
Measured data simultaneously, makes a dry blood cake card and determines targeted vitamins concentration using the dry blood cake detection method of the present invention.
Two groups of data are subjected to correlation analysis, two groups of data dependences are shown in Figure 10 to Figure 18, from related coefficient as can be seen that two kinds of inspections
Result obtained by survey method has stronger correlation, therefore, the testing result of dry blood cake detection method of the invention, precision with
Conventional new blood vitamin detection method is similar, but the dry blood cake detection method of the present invention can detect multivitamin simultaneously,
It is time saving and energy saving.
In conclusion conventional new blood vitamin detection method needs the new blood of 200 μ L, transported in the preservation of sample
Defeated aspect condition is harsh, and detection method is very cumbersome, and compared with conventional new blood vitamin detection method, of the invention is dry
Blood cake detection method has a clear superiority, and dry blood cake card is protected from light at shady and cool drying after blood sampling and preserves, and transportation cost is low (only
It need to be protected from light in packaging in sealing and add in drier, antioxidant).Conventional new blood vitamin detection method needs 200 μ L's
New blood, and the dry blood cake detection method of the present invention need to only take the new blood of 15 μ L, blood sampling volume is lower.It is conventional new
Fresh blood vitamin detection method labor intensive, and the dry blood cake detection method of the present invention realizes full-automatic extraction, avoids artificial
Occur the possibility of mistake in extraction process, greatly improve the repeatability of extraction efficiency.Conventional new blood vitamin inspection
Survey method takes longer, and the time cost of dry blood cake detection method of the present invention is only used for liquid phase mass spectrometric analysis, is analyzing
While dry blood cake extraction platform carry out extraction operation, be equivalent to zero-time cost, can realize high throughput sample analysis (hundreds of
Sample extracts detection simultaneously, and night does not stop work operation).
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. multivitamin detection method in a kind of dry blood cake, which is characterized in that include the following steps：
S101：Internal standard compound, dry blood cake after being handled are added in dry blood cake；
S102：Blood cake dry after processing is extracted with the first extract liquor, dry blood cake is used after obtaining the first detection liquid or handling
Second extract liquor is extracted, and obtains the second detection liquid；
S103：The described first detection liquid or the second detection liquid are detected using liquid chromatograph-mass spectrometer；
Wherein, first extract liquor is the methanol aqueous solution that percent by volume is 5%-95%；Second extract liquor is 10-
90% methanol aqueous solution.
2. multivitamin detection method in dry blood cake according to claim 1, which is characterized in that the internal standard compound tool
Body be Isotopic Internal Standard liquid, the Isotopic Internal Standard liquid 5-40 μ L.
3. multivitamin detection method in dry blood cake according to claim 1, which is characterized in that first extraction
Liquid is 5-100 μ L, and the extraction flow velocity of first extract liquor is 10-150 μ L/ml.
4. multivitamin detection method in dry blood cake according to claim 1, which is characterized in that second extraction
Liquid is 5-100 μ L, and the extraction flow velocity of second extract liquor is 10-150 μ L/ml.
5. multivitamin detection method in dry blood cake according to claim 1, which is characterized in that the S101 is also wrapped
It includes：Processing, drying time 1-60s is dried in blood cake dry after processing.
6. a kind of multivitamin automatic checkout system in dry blood cake, which is characterized in that including：Pretreatment unit, extraction are single
Member and detection unit；
The pretreatment unit is used to add in internal standard compound, dry blood cake after being handled to dry blood cake；
The extraction cells are used for dry blood cake after handling and are extracted with the first extract liquor, obtain the first detection liquid or will locate
Dry blood cake is extracted with the second extract liquor after reason, obtains the second detection liquid；
The detection unit examines the described first detection liquid or the second detection liquid using liquid chromatograph-mass spectrometer
It surveys.
7. multivitamin automatic checkout system in dry blood cake according to claim 6, which is characterized in that further include：
Cleaning unit, the cleaning unit are used to clean the extraction cells.
8. multivitamin automatic checkout system in dry blood cake according to claim 7, which is characterized in that the extraction
When unit is extracted using the first extract liquor, cleaning unit is specially that the outlet to the extraction cells is extracted using first
Liquid, cleaning solution and the first extract liquor carry out wash cycles, and the wash cycles program is：
S201：The outlet of the extraction cells is cleaned using first extract liquor, to clean for the first time；
S202：The outlet of extraction cells after being cleaned to first time is cleaned using the cleaning solution, is cleaned for second；
S203：The outlet of extraction cells after being cleaned to second is cleaned using first extract liquor, is cleaned for third time；
The import of the extraction cells carries out single cleaning using the first extract liquor.
9. multivitamin automatic checkout system in dry blood cake according to claim 7, which is characterized in that the extraction
When unit is extracted using the second extract liquor, cleaning unit is specially that the outlet to the extraction cells is extracted using second
Liquid, cleaning solution and the second extract liquor carry out wash cycles, and the wash cycles program is：
S301：The outlet of the extraction cells is cleaned using second extract liquor, to clean for the first time；
S302：The outlet of extraction cells after being cleaned to first time is cleaned using the cleaning solution, is cleaned for second；
S303：The outlet of extraction cells after being cleaned to second is cleaned using second extract liquor, is cleaned for third time；
The import of the extraction cells carries out single cleaning using the second extract liquor.
10. multivitamin automatic checkout system in dry blood cake according to claim 8 or claim 9, which is characterized in that described
Cleaning solution is specially acetone：Isopropanol：Methanol：The mixed liquor of water, wherein, the acetone：The isopropanol：The methanol：Institute
The volume ratio for stating water is 1:1:1:1.
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| CN110146626A (en) * | 2019-06-18 | 2019-08-20 | 上海可力梅塔生物医药科技有限公司 | A kind of method of folic acid in high performance liquid chromatography string mass Spectrometry for Determination blood cake |
| CN110174476A (en) * | 2019-06-10 | 2019-08-27 | 合肥谱佳医学检验实验室有限公司 | The detection method of the Liquid Chromatography-Tandem Mass Spectrometry of a variety of liposoluble vitamins in a kind of dried blood spot |
| US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
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| US10656059B2 (en) | 2018-03-07 | 2020-05-19 | Alcala Pharmaceutical, Inc. | Method for qualitative and quantitative multiplexing of drug analytes from biological samples |
| US11054349B2 (en) | 2018-03-07 | 2021-07-06 | Alcala Pharmaceutical, Inc. | Method for preparation of dried blood sample for multiplexing of analytes |
| CN110174476A (en) * | 2019-06-10 | 2019-08-27 | 合肥谱佳医学检验实验室有限公司 | The detection method of the Liquid Chromatography-Tandem Mass Spectrometry of a variety of liposoluble vitamins in a kind of dried blood spot |
| CN110146628A (en) * | 2019-06-18 | 2019-08-20 | 上海可力梅塔生物医药科技有限公司 | A kind of high performance liquid chromatography string mass-spectrometric technique detects the kit of folic acid in blood cake |
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| CN114755323A (en) * | 2022-03-24 | 2022-07-15 | 北京和合医学诊断技术股份有限公司 | Method and kit for detecting content of fat-soluble vitamins in dried blood paper |
| CN115343379A (en) * | 2022-04-20 | 2022-11-15 | 杭州谱聚医疗科技有限公司 | Preparation method of blank blood matrix and standard substance and detection method of vitamin |
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