CN108195902B - Metal sulfide ore biosensor and use method thereof - Google Patents
Metal sulfide ore biosensor and use method thereof Download PDFInfo
- Publication number
- CN108195902B CN108195902B CN201711380901.8A CN201711380901A CN108195902B CN 108195902 B CN108195902 B CN 108195902B CN 201711380901 A CN201711380901 A CN 201711380901A CN 108195902 B CN108195902 B CN 108195902B
- Authority
- CN
- China
- Prior art keywords
- biosensor
- metal sulfide
- solution
- electrode
- detected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229910052976 metal sulfide Inorganic materials 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 18
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 99
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 82
- 238000001514 detection method Methods 0.000 claims abstract description 51
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 39
- 239000008103 glucose Substances 0.000 claims abstract description 39
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 35
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 34
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 34
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 12
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 11
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 10
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims abstract description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 20
- CWQXQMHSOZUFJS-UHFFFAOYSA-N molybdenum disulfide Chemical compound S=[Mo]=S CWQXQMHSOZUFJS-UHFFFAOYSA-N 0.000 claims description 15
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 10
- 229910052697 platinum Inorganic materials 0.000 claims description 10
- 229910052709 silver Inorganic materials 0.000 claims description 10
- 239000004332 silver Substances 0.000 claims description 10
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 4
- 239000011707 mineral Substances 0.000 claims 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 66
- 239000000243 solution Substances 0.000 description 30
- 229910052951 chalcopyrite Inorganic materials 0.000 description 23
- DVRDHUBQLOKMHZ-UHFFFAOYSA-N chalcopyrite Chemical compound [S-2].[S-2].[Fe+2].[Cu+2] DVRDHUBQLOKMHZ-UHFFFAOYSA-N 0.000 description 23
- 229910052683 pyrite Inorganic materials 0.000 description 23
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 23
- 239000011028 pyrite Substances 0.000 description 23
- XCAUINMIESBTBL-UHFFFAOYSA-N lead(ii) sulfide Chemical compound [Pb]=S XCAUINMIESBTBL-UHFFFAOYSA-N 0.000 description 19
- 229910052949 galena Inorganic materials 0.000 description 18
- 229910052961 molybdenite Inorganic materials 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 9
- 239000000758 substrate Substances 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 description 7
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 description 7
- 239000003792 electrolyte Substances 0.000 description 7
- 230000006698 induction Effects 0.000 description 7
- 238000011835 investigation Methods 0.000 description 7
- 229940116269 uric acid Drugs 0.000 description 7
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical compound OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 description 6
- 229930091371 Fructose Natural products 0.000 description 4
- 239000005715 Fructose Substances 0.000 description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 4
- BERDEBHAJNAUOM-UHFFFAOYSA-N copper(I) oxide Inorganic materials [Cu]O[Cu] BERDEBHAJNAUOM-UHFFFAOYSA-N 0.000 description 4
- KRFJLUBVMFXRPN-UHFFFAOYSA-N cuprous oxide Chemical compound [O-2].[Cu+].[Cu+] KRFJLUBVMFXRPN-UHFFFAOYSA-N 0.000 description 4
- 229940112669 cuprous oxide Drugs 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 238000002848 electrochemical method Methods 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000002070 nanowire Substances 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 229920000557 Nafion® Polymers 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 206010047623 Vitamin C deficiency Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- CAMXVZOXBADHNJ-UHFFFAOYSA-N ammonium nitrite Chemical compound [NH4+].[O-]N=O CAMXVZOXBADHNJ-UHFFFAOYSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007772 electrode material Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000002343 gold Chemical class 0.000 description 1
- 229960002163 hydrogen peroxide Drugs 0.000 description 1
- 238000001027 hydrothermal synthesis Methods 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 150000002815 nickel Chemical class 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005289 physical deposition Methods 0.000 description 1
- 238000005498 polishing Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 208000010233 scurvy Diseases 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000005979 thermal decomposition reaction Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a metal sulfide ore biosensor and a using method thereof, the metal sulfide ore biosensor comprises a cylindrical sensing element and a copper wire connected with the bottom surface of one end of a cylinder, the sensing element is metal sulfide ore, the sensing element and the copper wire are covered with polytetrafluoroethylene, the bottom surface of one end of the sensing element, which is not covered with polytetrafluoroethylene, is a detection end and is contacted with a solution to be detected, and the solution to be detected is hydrogen peroxide, ascorbic acid or glucose solution. The application method comprises the steps of taking the metal sulfide ore biosensor as a working electrode, establishing a three-electrode system of the working electrode, a counter electrode and a reference electrode, and connecting the three-electrode system with an electrochemical workstation; and then the detection end of the working electrode is placed in the solution to be detected, the magnitude of the response current in the solution to be detected is detected through an electrochemical workstation, and then the concentration of the biological components in the solution to be detected is determined according to a standard curve. The invention has the advantages that: the device has the advantages of simple structure, little pollution, low cost, high response speed, wide detection range and good operation stability.
Description
Technical Field
The invention belongs to the technical field of biosensors for detecting biomedical data, and particularly relates to a metal sulfide ore biosensor using natural metal sulfide ore as a working electrode and a using method thereof.
Background
In recent decades, most biosensors immobilize a biological enzyme on an electrode and detect a corresponding substrate. The purification process of the biological enzyme is difficult, the process of solidifying the biological enzyme on the electrode is also complicated and complex, the cost is high, and the biological enzyme is easy to lose activity under the influence of the external environment, so that the biological enzyme sensor has the defects of poor stability, short service life and the like. With the rapid development of technology, biosensors have been developed into the fourth generation, enzyme-free sensors. The enzyme-free sensor has the following advantages: firstly, the preparation becomes relatively simple and easy to operate; secondly, the service life is relatively long, and the stability is good; and thirdly, under the condition of no enzyme, the influence of dissolved oxygen on some substrates is avoided. Therefore, compared with the enzyme electrochemical biosensor, the enzyme-free electrochemical biosensor has the advantages of wide detection linear range, good stability, low cost, long service life and the like.
Examples of glucose detection methods include a spectroscopic method, a chromatographic method, and an electrochemical method, and among them, an electrochemical glucose sensor is widely used because of its advantages such as high detection speed, high sensitivity, low cost, and easy operation.
Publication (publication) No.: CN106198654A discloses an enzyme-free electrochemical glucose sensor electrode material, a preparation method and application thereof, and provides FeS doped with nickel2The coating material is coated on the conductive glass, and the prepared glucose sensor has the advantages of wide detection range, strong anti-interference performance, low cost and the like. However, the preparation method is relatively complex, a plurality of raw materials of a mixed solution of ferrous salt, nickel salt, citrate, polyvinylpyrrolidone, sodium hydroxide solution, sulfur powder and water are required to be used, the raw materials are obtained by a hydrothermal method, and the preparation process needs to be carried out for 20-48 hours at high temperature in a high-pressure reaction kettle.
Hydrogen peroxide (H)2O2) Can be used as an oxidant in medical, pharmaceutical and food industries and is also a mediator of a plurality of biological systems; however, excessive hydrogen peroxide can cause damage to the central nervous system of humansAnd (4) damaging. Currently, there are many methods for detecting hydrogen peroxide, such as titration, spectrophotometry, and electrochemical methods. Among them, the electrochemical method for detecting hydrogen peroxide has the advantages of high sensitivity, good selectivity, simple method, etc., and is widely applied to the determination of hydrogen peroxide.
Publication (publication) No.: CN102735732A discloses preparation and application of a nano cuprous oxide enzyme-free hydrogen peroxide sensor electrode. The cuprous oxide nanowire and the Nafion modified gold electrode are used for detecting the content of the hydrogen peroxide by utilizing the catalytic property of the cuprous oxide, so that the rapid electrochemical determination of the hydrogen peroxide is realized, and the method has the advantages of low detection limit, high sensitivity and good stability. The sensor has relatively complex preparation process, the aluminum oxide template needs to be electrodeposited and removed in alkaline solution, and the used cuprous oxide nanowires and gold electrodes are expensive and are not suitable for industrialization.
Publication (publication) No.: CN105738440A discloses a gold nano-array electrode and an enzyme-free hydrogen peroxide sensor prepared by the same. According to the method, a silicon-based single-layer polymer colloidal crystal array is prepared, a gold film is deposited on the surface of a template by a physical deposition method, and then the template is subjected to thermal decomposition and annealing treatment to obtain the silicon-based gold nano array. The preparation method has the defects of complicated steps, high cost and the like, and needs to use an organic solvent, which is not beneficial to environmental protection.
L ascorbic acid (vitamin C, abbreviated as Vc) can block the formation of carcinogenic ammonium nitrite, lack of Vc can cause scurvy, and excessive Vc can also have adverse effect on human body.
Disclosure of Invention
The invention aims to solve the problems that the preparation process of an enzyme-free sensor is still complex and the cost is still high, and provides a metal sulfide ore biosensor taking natural metal sulfide ore as an induction element and a using method thereof.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the invention discloses a metal sulfide ore biosensor, which comprises a cylindrical induction element and a copper wire connected with the bottom surface of one end of a cylinder, and is characterized in that: the sensing element is a metal sulfide ore, the side surface of the cylinder is packaged with polytetrafluoroethylene, the copper wire and the bottom surface connected with the copper wire are also packaged with polytetrafluoroethylene, and the bottom surface at the other end is a detection end contacted with a solution to be detected.
The induction element is natural metal sulfide ore which is polished, cleaned and processed, the diameter of the induction element is 3 mm-8 mm, and the height of the induction element is 3 mm-8 mm.
The metal sulfide ores comprise natural pyrite, natural chalcopyrite, natural galena and natural molybdenite.
The metal sulfide ore biosensor is used for quantitatively detecting biological components in a biological sample, wherein the biological components comprise hydrogen peroxide, ascorbic acid or glucose.
The invention relates to an application of natural metal sulfide ore, which is characterized in that: for the quantitative detection of biological components in biological samples, said biological components comprising hydrogen peroxide, ascorbic acid or glucose.
The invention discloses a using method of a metal sulfide ore biosensor, which is characterized by comprising the following steps of:
(1) establishing a three-electrode system by taking the metal sulfide ore biosensor as a working electrode, a platinum electrode as a counter electrode and silver/silver chloride as a reference electrode, wherein the three-electrode system is connected with an electrochemical workstation;
(2) and placing the detection end of the metal sulfide ore biosensor in a solution to be detected, detecting the response current of the sensor to the biological components in the solution to be detected through an electrochemical workstation, and determining the concentration of the biological components in the solution to be detected according to a standard curve of the response current and the concentration of the biological components.
The principle of the invention is that almost all metal sulfide ores in nature have the properties of semiconductors and conductivity. Based on the characteristics, the invention adopts natural metal sulfide ore as the sensing element of the biosensor directly to quantitatively detect hydrogen peroxide, ascorbic acid or glucose in a biological sample.
Compared with the prior art, the invention has the following advantages:
1. the metal sulfide ore biosensor has the advantages of simple preparation method, low pollution and low cost.
2. The metal sulfide ore biosensor has the advantages of high response speed to biological components to be detected, wide detection range, good operation stability and long service life.
Drawings
FIG. 1 is a schematic structural view of a metal sulfide ore biosensor.
Wherein, 1, copper conductor, 2, polytetrafluoroethylene, 3, response original part.
FIG. 2 is a schematic diagram of a biosensor detection system for metal sulfide ores.
The electrochemical measuring device comprises a working electrode, a counter electrode, a reference electrode, a solution to be measured, and an electrochemical workstation, wherein the working electrode is 4, the counter electrode is 5, the reference electrode is 6, the solution to be measured is 7, and the electrochemical workstation is 8.
Fig. 3 is a standard curve of the response current of the pyrite biosensor in relation to the concentration of hydrogen peroxide.
FIG. 4 is a standard curve of the response current of the chalcopyrite biosensor in relation to the concentration of hydrogen peroxide.
Fig. 5 is a standard curve of the response current of the pyrite biosensor in relation to the concentration of ascorbic acid.
FIG. 6 is a standard curve of chalcopyrite biosensor response current versus ascorbic acid concentration.
FIG. 7 is a standard curve of the response current of galena biosensor as a function of hydrogen peroxide concentration.
FIG. 8 is a standard curve of the response current of galena biosensor as a function of glucose concentration.
FIG. 9 is a standard curve of response current of a molybdenite biosensor as a function of glucose concentration.
Detailed Description
The present invention will be described in further detail with reference to the following examples in conjunction with the accompanying drawings.
The raw materials used in the following examples were all purchased from Shanxi province, metal sulfide ores were purchased from Shanxi province, and polytetrafluoroethylene was purchased from Tianjin Idida Heng Cheng scientific and technological development Co., Ltd.
Example 1
As shown in fig. 1-2, the biosensor for metal sulfide ore comprises a cylindrical sensing element 3 and a copper wire 1 connected with the bottom surface of one end of the cylinder, and is characterized in that: the induction element 3 is a metal sulfide ore, the side surface of the cylinder is packaged with polytetrafluoroethylene 2, the copper wire 1 and the bottom surface connected with the copper wire 1 are also packaged with polytetrafluoroethylene 2, and the bottom surface of the other end is a detection end contacted with a solution to be detected.
The induction element 3 is formed by polishing, cleaning and processing metal sulfide ores, and has the diameter of 5mm and the height of 5 mm.
The metal sulfide ores are pyrite, chalcopyrite, galena and molybdenite respectively.
As shown in FIG. 2, the method for using the metal sulfide ore biosensor comprises the following detection steps:
(1) establishing a three-electrode system by taking a metal sulfide ore biosensor as a working electrode 4, a platinum electrode as a counter electrode 5 and silver/silver chloride as a reference electrode 6, wherein the three-electrode system is connected with an electrochemical workstation 8;
(2) the detection end of the working electrode 4 is placed in the solution 7 to be detected, the magnitude of the response current of the sensor to the biological components in the solution 7 to be detected is detected through the electrochemical workstation 8, and then the concentration of the biological components in the solution 7 to be detected is determined according to the standard curve of the response current and the concentration of the biological components.
Example 2
As shown in fig. 2, the steps of the pyrite biosensor to detect the concentration of hydrogen peroxide are as follows:
(1) a three-electrode system is established by taking a pyrite biosensor as a working electrode 4, a platinum electrode as a counter electrode 5 and silver/silver chloride as a reference electrode 6, and is connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used is sodium hydroxide (NaOH) solution, the concentration of NaOH is 100mM, the voltage is-0.6V, and the response current of the pyrite biosensor to the hydrogen peroxide concentration is maximum under the condition.
(2) The detection end of the working electrode 4 is placed in the hydrogen peroxide solution 7, the magnitude of the response current of the pyrite biosensor to the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is detected through the electrochemical workstation 8, and then the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is determined according to the standard curve of the response current and the hydrogen peroxide concentration.
The standard curve of the response current of the pyrite biosensor in relation to the concentration of hydrogen peroxide is shown in fig. 3.
Specific selectivity investigation of pyrite biosensor:
the uric acid, the glucose and the fructose with the same concentration as the hydrogen peroxide are used for detection, and the pyrite biosensor has no obvious detection signals for the three substrates, and has better selectivity.
The detection performance of the pyrite biosensor on hydrogen peroxide is shown in table 1:
TABLE 1 detection Performance of pyrite biosensor for Hydrogen peroxide
Example 3
As shown in fig. 2, the steps of the chalcopyrite biosensor for detecting the hydrogen peroxide concentration are as follows:
(1) a chalcopyrite biosensor is used as a working electrode 4, a platinum electrode is used as a counter electrode 5, silver/silver chloride is used as a reference electrode 6, a three-electrode system is established, and the three-electrode system is connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used is sodium hydroxide (NaOH) solution, the concentration of NaOH is 100mM, the voltage is-0.4V, and the chalcopyrite biosensor has the maximum response current to the hydrogen peroxide concentration under the condition.
(2) The detection end of the working electrode 4 is placed in a hydrogen peroxide solution 7, the magnitude of the response current of the chalcopyrite biosensor to the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is detected through an electrochemical workstation 8, and then the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is determined according to a standard curve of the response current and the hydrogen peroxide concentration.
The standard curve of the chalcopyrite biosensor response current and hydrogen peroxide concentration is shown in figure 4.
Specific selectivity investigation of chalcopyrite biosensor:
the method is characterized in that uric acid, glucose and fructose with the same concentration as hydrogen peroxide are used for detection, a chalcopyrite biosensor has no obvious detection signals for the three substrates, and the chalcopyrite biosensor has good selectivity.
The detection performance of the chalcopyrite biosensor on hydrogen peroxide is shown in the table 2:
TABLE 2 detection Performance of chalcopyrite biosensor for Hydrogen peroxide
Example 4
As shown in fig. 2, the steps of the pyrite biosensor for detecting the concentration of ascorbic acid (Vc) are as follows:
(1) a three-electrode system is established by taking a pyrite biosensor as a working electrode 4, a platinum electrode as a counter electrode 5 and silver/silver chloride as a reference electrode 6, and is connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used was a sodium hydroxide (NaOH) solution, the NaOH concentration was 100mM, and the voltage was-0.05V, under which the pyrite biosensor responded to the ascorbic acid concentration with the maximum and stable current.
(2) The detection end of the working electrode 4 is placed in a solution 7 for detecting ascorbic acid (Vc), the size of a response current of a pyrite biosensor against the concentration of the ascorbic acid (Vc) in the solution 7 for detecting the ascorbic acid (Vc) is detected through an electrochemical workstation 8, and then the concentration of the ascorbic acid (Vc) in the solution 7 for detecting the ascorbic acid (Vc) is determined according to a standard curve of the response current and the concentration of the ascorbic acid.
The standard curve of the response current of the pyrite biosensor as a function of the ascorbic acid concentration is shown in fig. 5.
Specific selectivity investigation of pyrite biosensor:
the uric acid, the glucose and the fructose with the same concentration as the ascorbic acid (Vc) are used for detection, and the pyrite biosensor has no obvious detection signals for the three substrates, so that the pyrite biosensor has better selectivity.
The detection performance of the pyrite biosensor against ascorbic acid is shown in table 3:
TABLE 3 detection Performance of pyrite biosensor against ascorbic acid
Example 5
As shown in fig. 2, the steps of the chalcopyrite biosensor for detecting the concentration of ascorbic acid (Vc) are as follows:
(1) a chalcopyrite biosensor is used as a working electrode 4, a platinum electrode is used as a counter electrode 5, silver/silver chloride is used as a reference electrode 6, a three-electrode system is established, and the three-electrode system is connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used is sodium hydroxide (NaOH) solution, the concentration of NaOH is 100mM, the voltage is-0.05V, and the chalcopyrite biosensor has the maximum and stable current response to the ascorbic acid concentration under the condition.
(2) The detection end of the working electrode 4 is placed in an ascorbic acid (Vc) solution 7, the size of the response current of the chalcopyrite biosensor to the ascorbic acid (Vc) concentration in the ascorbic acid (Vc) solution 7 is detected through an electrochemical workstation 8, and then the ascorbic acid (Vc) concentration in the ascorbic acid (Vc) solution 7 is determined according to a standard curve of the response current and the ascorbic acid concentration.
The standard curve of the chalcopyrite biosensor response current versus ascorbic acid concentration is shown in figure 6.
Specific selectivity investigation of chalcopyrite biosensor:
the detection is carried out by using uric acid, glucose and fructose with the same concentration as ascorbic acid (Vc), and a chalcopyrite biosensor has no obvious detection signals for the three substrates and has better selectivity.
The detection performance of the chalcopyrite biosensor against ascorbic acid is shown in table 4:
TABLE 4 chalcopyrite biosensor detection Performance against ascorbic acid
Example 6
As shown in fig. 2, the steps of the galena biosensor for detecting the hydrogen peroxide concentration are as follows:
(1) a square lead ore biosensor is used as a working electrode 4, a platinum electrode is used as a counter electrode 5, silver/silver chloride is used as a reference electrode 6, and a three-electrode system is established and connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used is sodium hydroxide (NaOH) solution, the concentration of NaOH is 100mM, the voltage is-0.4V, and under the condition, the lead ore biosensor has the maximum and stable current response to the hydrogen peroxide concentration.
(2) The detection end of the working electrode 4 is placed in a hydrogen peroxide solution 7, the magnitude of the response current of the galena biosensor to the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is detected through an electrochemical workstation 8, and then the hydrogen peroxide concentration in the hydrogen peroxide solution 7 is determined according to the standard curve of the response current and the hydrogen peroxide concentration.
The response current of galena biosensor is plotted against the hydrogen peroxide concentration in the standard curve of fig. 7.
Specific selectivity investigation of galena biosensors:
the method is characterized in that uric acid, ascorbic acid and catechol with the same concentration as hydrogen peroxide are used for detection, the galena biosensor has no obvious detection signals for the three substrates, and the galena biosensor has good selectivity.
The performance of the galena biosensor in detecting hydrogen peroxide is shown in table 5:
TABLE 5 detectivity of galena biosensors for hydrogen peroxide
Example 7
As shown in fig. 2, the steps of the galena biosensor for detecting the glucose concentration are as follows:
(1) a square lead ore biosensor is used as a working electrode 4, a platinum electrode is used as a counter electrode 5, silver/silver chloride is used as a reference electrode 6, and a three-electrode system is established and connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used was sodium hydroxide (NaOH) solution, the NaOH concentration was 100mM, the voltage was 0.4V, and the current response of the lead ore biosensor to the glucose concentration was maximal and stable under these conditions.
(2) The detection end of the working electrode 4 is placed in a glucose solution 7, the magnitude of the response current of the galena biosensor to the glucose concentration in the glucose solution 7 is detected through an electrochemical workstation 8, and then the glucose concentration in the glucose solution 7 is determined according to the standard curve of the response current and the glucose concentration.
The response current of galena biosensor is plotted against the glucose concentration in FIG. 8.
Specific selectivity investigation of galena biosensors:
uric acid, ascorbic acid and catechol with the same concentration as glucose are used for detection, and the galena biosensor has no obvious detection signals for the three substrates, and has good selectivity.
The detection performance of the galena biosensor on glucose is shown in table 6:
TABLE 6 detectivity of galena biosensors for glucose
Example 8
As shown in FIG. 2, the steps of the molybdenite biosensor for detecting the glucose concentration are as follows:
(1) a molybdenite biosensor is used as a working electrode 4, a platinum electrode is used as a counter electrode 5, silver/silver chloride is used as a reference electrode 6, and a three-electrode system is established and connected with an electrochemical workstation 8;
the working conditions are as follows: the electrolyte used was a sodium hydroxide (NaOH) solution, the NaOH concentration was 100mM, the voltage was 0.4V, and the molybdenite biosensor responded to the glucose concentration with a stable current.
(2) The detection end of the working electrode 4 is placed in a glucose solution 7, the response current of the molybdenite biosensor in the glucose solution 7 to the glucose concentration is detected through an electrochemical workstation 8, and then the glucose concentration in the glucose solution 7 is determined according to a standard curve of the response current and the glucose concentration.
The response current of the molybdenite biosensor is plotted against the glucose concentration in the standard curve of FIG. 9.
Specific selectivity investigation of molybdenite biosensors:
the molybdenite biosensor has the advantages that uric acid, hydrogen peroxide, ascorbic acid and catechol with the same concentration as glucose are used for detection, the molybdenite biosensor has no obvious detection signals for the four substrates, and the molybdenite biosensor has good selectivity.
The detection performance of the molybdenite biosensor on glucose is shown in table 7:
TABLE 7 detection Performance of molybdenite biosensor on glucose
Claims (3)
1. The use method of the metal sulfide ore biosensor is characterized by comprising the following steps:
(1) establishing a three-electrode system by taking the metal sulfide ore biosensor as a working electrode, a platinum electrode as a counter electrode and silver/silver chloride as a reference electrode, wherein the three-electrode system is connected with an electrochemical workstation;
metal sulphide ore biosensor, including the response original paper of cylinder type and the copper line that links to each other with cylinder one end circle cross-section, its characterized in that: the sensing element is a metal sulfide mineral, polytetrafluoroethylene is packaged on the side face of the cylinder, polytetrafluoroethylene is packaged on the copper wire and the circular section connected with the copper wire, the other circular section is a detection end in contact with a solution to be detected, and the metal sulfide mineral is a natural square lead mineral or a natural molybdenite;
(2) and placing the detection end of the metal sulfide ore biosensor in a solution to be detected, detecting the response current of the sensor to a certain biological component in the solution to be detected through an electrochemical workstation, and determining the concentration of the biological component in the solution to be detected according to the standard curve of the concentration of the biological component and the response current.
2. The use method of the metal sulfide ore biosensor as claimed in claim 1, wherein the sensing element is a polished, cleaned and processed natural metal sulfide mineral with a diameter of 3 mm-8 mm and a height of 3 mm-8 mm.
3. The method as claimed in claim 1, wherein the metal sulfide ore biosensor is used for quantitative detection of biological components in a biological sample, and the biological components include hydrogen peroxide, ascorbic acid or glucose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711380901.8A CN108195902B (en) | 2017-12-20 | 2017-12-20 | Metal sulfide ore biosensor and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711380901.8A CN108195902B (en) | 2017-12-20 | 2017-12-20 | Metal sulfide ore biosensor and use method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108195902A CN108195902A (en) | 2018-06-22 |
| CN108195902B true CN108195902B (en) | 2020-08-07 |
Family
ID=62577084
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711380901.8A Active CN108195902B (en) | 2017-12-20 | 2017-12-20 | Metal sulfide ore biosensor and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108195902B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103207225A (en) * | 2013-03-14 | 2013-07-17 | 华中科技大学 | Electrochemical transducer probe for detecting chemical oxygen demand and manufacturing method of electrochemical transducer probe |
-
2017
- 2017-12-20 CN CN201711380901.8A patent/CN108195902B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103207225A (en) * | 2013-03-14 | 2013-07-17 | 华中科技大学 | Electrochemical transducer probe for detecting chemical oxygen demand and manufacturing method of electrochemical transducer probe |
Non-Patent Citations (4)
| Title |
|---|
| Application of Pyrite and Chalcopyrite Electrodes for the Acid-Base Determinations in Nitriles;Simi, Stani et al.;《Journal of the Brazilian Chemical Society》;20110118;第22卷(第4期);全文 * |
| Natural Sulphide Minerals as Sensors for Determination of Total Acidity of Humic and Fulvic Acids;Antonijevic, Simic et al.;《Sensor Letters》;20090801;第7卷(第4期);全文 * |
| Potentiometric determination of ascorbic acid in water–acetonitrile solution using pyrite and chalcopyrite electrodes;Stanic, Stepanovic;《Journal of Solid State Electrochemistry》;20160624;第20卷(第10期);第2879-2893页 * |
| Stanic, Stepanovic.Potentiometric determination of ascorbic acid in water–acetonitrile solution using pyrite and chalcopyrite electrodes.《Journal of Solid State Electrochemistry》.2016,第20卷(第10期),2879–2893. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108195902A (en) | 2018-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kumar et al. | Non-enzymatic electrochemical detection of urea on silver nanoparticles anchored nitrogen-doped single-walled carbon nanotube modified electrode | |
| Emran et al. | Facile synthesis of microporous sulfur-doped carbon spheres as electrodes for ultrasensitive detection of ascorbic acid in food and pharmaceutical products | |
| CN106383158B (en) | A kind of hydrogen peroxide based on silver-graphene nano-complex is without enzyme sensor and preparation method thereof | |
| Chen et al. | A novel nonenzymatic electrochemical glucose sensor modified with Ni/Al layered double hydroxide | |
| Bao et al. | A sensitive and selective non-enzymatic glucose sensor based on AuNPs/CuO NWs-MoS2 modified electrode | |
| CN108732216B (en) | Electrochemical reduction graphene oxide modified electrode and application thereof in detection of heavy metal hexavalent chromium ions in water | |
| CN112110439B (en) | Preparation method and application of carbon nanotube wrapped nitrogen-doped porous carbon composite material | |
| CN107462620B (en) | Based on graphene/ZnO/ nickel foam nanocomposite glucose sensor electrode | |
| Baikeli et al. | Simultaneous determination of dopamine and uric acid using glassy carbon electrode modified with almond-shell-based nanoporous carbon | |
| Zhan et al. | A novel epinephrine biosensor based on gold nanoparticles coordinated polydopamine-functionalized acupuncture needle microelectrode | |
| Wei et al. | Voltammetric determination of copper in seawater at a glassy carbon disk electrode modified with Au@ MnO 2 core-shell microspheres | |
| Hadi et al. | Sensitive detection of histamine at metal-organic framework (Ni-BTC) crystals and multi-walled carbon nanotubes modified glassy carbon electrode | |
| CN108387624B (en) | Three-dimensional porous carbon/polysulfide cordierite compound modified electrode and preparation and application thereof | |
| Wang et al. | Electrochemical determination of lead (II) in environmental waters using a sulfydryl modified covalent organic framework by square wave anodic stripping voltammetry (SWASV) | |
| CN108195902B (en) | Metal sulfide ore biosensor and use method thereof | |
| CN110841664B (en) | Cu2O @ BiOI composite material and preparation method and application thereof | |
| CN114878648B (en) | Cysteine electrochemical sensor and preparation method and application thereof | |
| CN114778638B (en) | Photoelectrochemical sensor for detecting acrylamide and preparation method and application thereof | |
| Jose et al. | Electrochemical glucose sensing using molecularly imprinted polyaniline–copper oxide coated electrode | |
| CN110672685B (en) | Application of carbon fiber microelectrode in hydroquinone detection | |
| CN114858887A (en) | Construction of gold disk electrode-based miniaturized electrochemical aptamer detection platform | |
| CN110208356B (en) | Electrochemical sensor and preparation and application thereof | |
| CN109187698B (en) | Hydrogen peroxide electrochemical sensor based on nickel sulfide nanoenzyme | |
| CN109613084B (en) | High-sensitivity detection H of nano gold-protoporphyrin zinc (II)2O2Construction and application of electrochemical sensor | |
| Kakhki | Recent developments on the modification of graphite electrodes with nanoparticles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240811 Address after: No. 93, 24th Floor, Building 47, Keji Road, Tiedong District, Anshan City, Liaoning Province, 114001 Patentee after: Liaoning Rongchuang Meida Technology Co.,Ltd. Country or region after: China Address before: 114051 No. 185 Qianshan Middle Road, hi tech Zone, Liaoning, Anshan Patentee before: University of Science and Technology Liaoning Country or region before: China |
|
| TR01 | Transfer of patent right |