CN108195724B - Measuring method and measuring device for grain component content - Google Patents
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- 238000000034 method Methods 0.000 title claims abstract description 49
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 146
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- 238000005259 measurement Methods 0.000 claims 1
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Abstract
The invention provides a method and a device for measuring the content of seed components. The method comprises the following steps: obtaining the effective volume and weight of the seeds; acquiring the density of a kernel embryo and the density of kernel endosperm; and calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm. The device comprises: the first acquisition unit is used for acquiring the effective volume and weight of the seeds; the second acquisition unit is used for acquiring the density of the kernel embryo and acquiring the density of the kernel endosperm; the first calculating unit is used for calculating the volume of the obtained kernel embryo and the volume of the kernel endosperm to obtain the volume ratio of the embryo in the kernel and the volume ratio of the endosperm.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to a method and a device for measuring the content of seed components.
Background
The grain mainly comprises embryo, endosperm, seed coat and the like on the tissue part, wherein the endosperm and the embryo part are formed by the gradual differentiation and development of single cells, and in the process of grain differentiation and development, a stem vascular tissue and a caryopsis nutrient transport tissue are required to transport nutrients so as to ensure the differentiation and development process. The final size and morphological structure of the differentiated development of endosperm and embryo are often influenced by genetic and environmental factors. The quality of the seeds is detected and identified by researching the size and the structure of endosperm and embryo in the seeds.
At present, the morphological anatomical information of the internal tissue structure of grains is obtained by performing CT scanning on the grains, and the image of the grains is segmented and the structures of the embryo and the endosperm are extracted by three-dimensional image processing software.
However, because the density difference between the embryo and the endosperm in the kernel is not large, when three-dimensional image software is adopted for processing, the dividing line between the embryo and the endosperm cannot be accurately determined, so that the dividing precision of the embryo and the endosperm in the kernel is reduced; and when three-dimensional image software is adopted for processing, the processing process is complex and tedious, and the detection efficiency is reduced.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a method and a device for measuring the content of seed components, which can improve the detection precision and the detection efficiency of the seed components.
In order to achieve the purpose, the invention provides the following technical scheme:
in one aspect, the present invention provides a method for measuring a content of a seed component, comprising:
obtaining the effective volume and weight of the seeds;
acquiring the density of a kernel embryo and the density of kernel endosperm;
and calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm.
Further, the method further comprises:
and calculating the volume ratio of the embryo and the endosperm in the kernel according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm.
Further, the step of calculating the volume fraction of embryos and the volume fraction of endosperm in the kernel according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm comprises:
the volume of the embryo in the kernel is the ratio of the volume of the embryo in the kernel to the effective volume of the kernel;
the volume of endosperm in the kernel is the ratio of the volume of endosperm in the kernel to the effective volume of the kernel.
Further, the effective volume of the kernel is: the volume difference between the total volume of the kernel and the cavity volume of the kernel.
Further, the step of obtaining the effective volume of the grains comprises:
performing CT scanning on the grains to obtain CT sequence images of the grains;
and performing three-dimensional threshold segmentation on the CT sequence image to obtain a cavity volume in the seed and the total volume of the seed, wherein the volume difference between the total volume and the cavity volume is the effective volume of the seed.
Further, the step of obtaining the density of the kernel embryo and obtaining the density of the kernel endosperm comprises:
sampling the grains to obtain an embryo sample block and an endosperm sample block;
calculating the volume and weight of the embryo sample piece and calculating the volume and weight of the endosperm sample piece;
calculating the density of the embryo from the volume and weight of the embryo sample piece and the density of the endosperm from the volume and weight of the endosperm sample piece;
wherein, the density of the embryo sample block is the same as that of the kernel embryo, and the density of the endosperm sample block is the same as that of the kernel endosperm.
Further, calculating the volume of the embryo sample piece and calculating the volume of the endosperm sample piece comprises the following steps:
respectively carrying out CT sequence scanning on the embryo sample block and the endosperm sample block to respectively obtain a CT sequence image of the embryo sample block and a CT sequence image of the endosperm sample block;
and respectively carrying out three-dimensional threshold segmentation on the CT sequence image of the embryo sample block and the CT sequence image of the endosperm sample block to respectively obtain the volume of the embryo sample block and the volume of the endosperm sample block.
Further, the step of calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm comprises:
calculating the volume of the kernel embryo and the volume of the kernel endosperm by the following formula:
V1+V2=V;
p1·V1+p2·V2=W;
wherein V1 is the volume of the kernel embryo, V2 is the volume of the kernel endosperm, V is the effective volume of the kernel, p1 is the density of the kernel embryo, p2 is the density of the kernel endosperm, and W is the weight of the kernel.
In another aspect, the present invention also provides a device for measuring the content of a seed component, comprising:
the first acquisition unit is used for acquiring the effective volume and weight of the seeds;
the second acquisition unit is used for acquiring the density of the kernel embryo and acquiring the density of the kernel endosperm;
and the first calculating unit is used for calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm.
Further, the measuring device further includes:
and the second calculation unit is used for calculating the volume ratio of the embryo in the kernel and the volume ratio of the endosperm according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm.
According to the technical scheme, the method and the device for measuring the content of the components of the grains acquire the effective volume and the weight of the grains; acquiring the density of a kernel embryo and the density of kernel endosperm; and calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm, so that the detection precision and the detection efficiency of the kernel component can be improved.
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In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic flow chart of a method for measuring a content of a grain component according to an embodiment of the present invention;
fig. 2 is a schematic flow chart of another method for measuring the content of a grain component according to an embodiment of the present invention;
fig. 3 is a schematic flow chart of one specific implementation of step S101 in the method for measuring a content of a grain component according to the embodiment of the present invention;
fig. 4 is a schematic structural diagram of a device for measuring a content of a grain component according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The method for measuring the content of the components of the grains provided by the embodiment of the invention is shown in figure 1, and specifically comprises the following steps:
s101: obtaining the effective volume and weight of the seeds;
in this step, the effective volume of the kernel refers to the volume of the kernel without the cavity, and mainly includes: the volume of embryo in kernel, the volume of endosperm in kernel, and the volume of seed coat. The effective volume of the grains is obtained by CT scanning of the grains and based on a three-dimensional image segmentation method. And weighing the kernel to obtain the weight of the kernel.
S102: acquiring the density of a kernel embryo and the density of kernel endosperm;
in this step, the kernel is destructively sampled to obtain an embryo sample block and an endosperm sample block, and the obtained embryo sample block and the obtained endosperm sample block are respectively weighed, so that the weight of the embryo sample block and the weight of the endosperm sample block can be obtained; the volume of the embryo sample block and the volume of the endosperm sample block are obtained based on a three-dimensional image segmentation method by performing CT scanning on the sample block. According to the weight and volume of the embryo sample block, the density of the embryo sample block can be calculated, and the density of the embryo sample block is also the density of the embryo in the kernel. The density of endosperm in the kernel can also be obtained by a method of calculating the density of the embryo.
S103: and calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm.
In this step, the density of embryos in the kernel and the density of endosperms in the kernel are obtained in the step S102, and the effective volume and the weight of the kernel are obtained in the step S101. And establishing a system of linear equations according to the four parameters, so that the volume of the embryo in the kernel and the volume of the endosperm in the kernel can be obtained.
The specific set of equations is as follows:
V1+V2=V;
p1·V1+p2·V2=W;
wherein V1 is the volume of the kernel embryo, V2 is the volume of the kernel endosperm, V is the effective volume of the kernel, p1 is the density of the kernel embryo, p2 is the density of the kernel endosperm, and W is the weight of the kernel.
From the above description, it can be seen that the method for measuring the content of the components of the grain provided by the embodiment of the present invention simply and rapidly obtains the accurate density of the embryo and the endosperm in the grain by sampling the grain, and obtains the volume of the embryo and the endosperm inside the grain by combining the effective volume and the weight of the grain. The problem that the segmentation precision when the embryo and the endosperm inside the kernel are segmented due to the fact that the segmentation line between the embryo and the endosperm cannot be accurately determined by adopting three-dimensional software processing and the complex and tedious processing process are effectively solved, and the detection precision and the detection efficiency of seed components are improved.
The embodiment of the invention provides a method for measuring the content of seed components. Referring to fig. 2, after the step S103, the method specifically includes the following steps:
s104: and calculating the volume ratio of the embryo and the endosperm in the kernel according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm.
In the step, the volume of the embryo in the kernel and the volume of the endosperm are accurately obtained through the embodiment of the method, and the volume ratio of the embryo in the kernel to the volume ratio of the endosperm can be directly obtained through calculation.
Wherein the volume ratio of the embryos in the seeds is the ratio of the volume of the embryos in the seeds to the effective volume of the seeds;
the volume of endosperm in the kernel is the ratio of the volume of endosperm in the kernel to the effective volume of the kernel.
As can be seen from the above description, the embodiment of the present invention can rapidly, directly and accurately obtain the characteristic parameters of the kernel by processing the volume of the embryo in the kernel and the volume of the endosperm in the kernel, which are obtained by the above embodiments: the volume fraction of embryos in the kernel and the volume fraction of endosperm in the kernel.
In an alternative embodiment, a specific implementation of step S101 is provided. Referring to fig. 3, the step S101 specifically includes the following steps:
s1011: performing CT scanning on the grains to obtain CT sequence images of the grains;
in the step, a CT scanning device is adopted to carry out 180-degree rotation scanning on the grains, the rotation step length is 0.6 degrees, and CT sequence images of the scanned grains are obtained.
S1012: and performing three-dimensional threshold segmentation on the CT sequence image to obtain a cavity volume in the seed and the total volume of the seed, wherein the volume difference between the total volume and the cavity volume is the effective volume of the seed.
In this step, based on the CT sequence image obtained in step S1011, the volume of the seed grain and the volume of the cavity in the seed grain are obtained based on a three-dimensional image segmentation method, and the effective volume of the seed grain is: the volume of the seed is different from the volume of the cavity in the seed, and the effective volume of the seed is equal to the sum of the volume of the embryo, the volume of the endosperm and the volume of the seed coat in the seed.
According to the description, the effective volume of the grains is obtained based on a three-dimensional image segmentation method, and the efficiency and the accuracy of detecting the effective volume of the grains are improved.
In an alternative embodiment, an embodiment of the above method for measuring the volume of an embryo sample block and the volume of an endosperm sample block is provided, which comprises the following steps:
s1: respectively carrying out CT sequence scanning on the embryo sample block and the endosperm sample block to respectively obtain a CT sequence image of the embryo sample block and a CT sequence image of the endosperm sample block;
s2: and respectively carrying out three-dimensional threshold segmentation on the CT sequence image of the embryo sample block and the CT sequence image of the endosperm sample block to respectively obtain the volume of the embryo sample block and the volume of the endosperm sample block.
From the above description, the volume of the kernel embryo sample block and the volume of the endosperm sample block are obtained based on a three-dimensional image segmentation method, so that the efficiency and accuracy of detecting the sample volume are improved.
In order to further explain the method of the invention in detail, the method provides a specific application example of the method for measuring the content of the seed grain component, and the specific contents are as follows:
and (3) carrying out high-resolution scanning imaging on the grains to be detected by using a microscopic CT scanning device to obtain three-dimensional image data of the grains, namely CT original data in tif format.
Wherein, the parameters of the micro CT scanning device are set as follows: the scanning voltage is 40kV, the current is 250 muA, the 2K mode is adopted, the resolution is 9.01μm, the distance from an X-ray light source to a detector is 215.601mm, the distance from the X-ray light source to grains is 107.000mm, 180-degree rotation scanning is carried out, and the rotation step length is 0.6 degrees.
And converting the CT original data into a section gray image in an 8-bit uncompressed BMP format.
Wherein, the image reconstruction is carried out in a module, the tomography image is converted into a section gray image in an 8-bit uncompressed BMP format by setting the following parameters,
the parameters are set as follows: dynamic image range-100-.
The effective volume of the seeds is 0.297cm by combining a three-dimensional threshold method3And measuring the weight of the grains by using a high-precision balance to be 0.3713 g.
Destructive sampling is carried out on the embryo and the endosperm of the complete kernel, an embryo sample block and an endosperm sample block in one kernel are respectively cut, and high-precision micro CT imaging is respectively carried out on the embryo sample block and the endosperm sample block.
Wherein, the parameters of the micro CT scanning device are set as follows: the scanning voltage is 40kV, the current is 250uA, the 2K mode is adopted, the resolution is 2.98 mu m, the distance from an X-ray light source to a detector is 280.611mm, the distance from the X-ray light source to an embryo or endosperm is 46.430mm, 180-degree rotation scanning is carried out, the rotation step is 0.6 degrees, and the CT raw data are converted into a cross-section gray image in an 8-bit uncompressed BMP format.
Based on the CT sequence images of the embryo sample block and the endosperm sample block, a three-dimensional voxel model is constructed, and the volumes of the embryo sample block and the endosperm sample block are calculated to be 6.93mm respectively3And 1.86mm3。
Respectively measuring the weights of the embryo sample block and the endosperm sample block by using a high-precision analytical balance, wherein the weights of the embryo sample block and the endosperm sample block are respectively 0.0083g and 0.0032g, and further calculating the real densities of the embryo and the endosperm which are respectively 1.19769g/cm3、1.72043g/cm3。
The weight W of the kernel, the effective volume V of the kernel, the density rho 1 of the embryo in the kernel and the density rho 2 of the endosperm in the kernel are known, and the volume V1 of the embryo in the kernel and the volume V2 of the endosperm can be calculated through the following equations, and the volume ratio of the embryo to the endosperm is calculated respectively;
the volume of the embryo and the volume of the endosperm in the kernel were calculated using the following formula:
V1+V2=V
ρ1V1+ρ2V2=W
the volume of the obtained kernel embryo is 0.029816cm3Endosperm volume of 0.2672cm3,
The ratio of the volume of embryo to the volume of endosperm was calculated according to the following formula:
the volume ratio of the kernel embryo is V1/V
Kernel endosperm volume ratio of V2/V
The volume of the obtained kernel embryo is 10.04 percent, and the volume of the kernel endosperm is 89.97 percent.
From the above description, the method effectively avoids the problem that the division line between the embryo and the endosperm cannot be accurately determined by adopting three-dimensional software processing, so that the division precision when the embryo and the endosperm inside the kernel are divided is reduced, and the complex and tedious processing process, and realizes the improvement of the detection precision and the detection efficiency of the seed components.
The embodiment of the invention provides a device for measuring the content of components of grains, and referring to fig. 4, the device specifically comprises:
the first acquiring unit 10 is used for acquiring the effective volume and weight of grains;
a second obtaining unit 20, configured to obtain a density of the kernel embryo and a density of the kernel endosperm;
the first calculating unit 30 is configured to calculate the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm.
The measuring device further includes:
and the second calculating unit 40 is used for calculating the volume ratio of the embryo in the kernel and the volume ratio of the endosperm according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm.
According to the technical scheme, the measuring device for the content of the grain components reduces the complex flow in the detection process, effectively improves the efficiency of seed detection and identification, has good repeatability and has important application value.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element. The terms "upper", "lower", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the description of the present invention, numerous specific details are set forth. It is understood, however, that embodiments of the invention may be practiced without these specific details. In some instances, well-known methods, structures and techniques have not been shown in detail in order not to obscure an understanding of this description. Similarly, it should be appreciated that in the foregoing description of exemplary embodiments of the invention, various features of the invention are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the disclosure and aiding in the understanding of one or more of the various inventive aspects. However, the disclosed method should not be interpreted as reflecting an intention that: that the invention as claimed requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment. Thus, the claims following the detailed description are hereby expressly incorporated into this detailed description, with each claim standing on its own as a separate embodiment of this invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention is not limited to any single aspect, nor is it limited to any single embodiment, nor is it limited to any combination and/or permutation of these aspects and/or embodiments. Moreover, each aspect and/or embodiment of the present invention may be utilized alone or in combination with one or more other aspects and/or embodiments thereof.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (4)
1. A method for measuring the content of a seed component, comprising:
obtaining the effective volume and weight of the seeds;
acquiring the density of a kernel embryo and the density of kernel endosperm;
calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm;
calculating the volume ratio of the embryo and the endosperm in the kernel according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm;
a step of obtaining an effective volume of grain, comprising: performing CT scanning on the grains to obtain CT sequence images of the grains; performing three-dimensional threshold segmentation on the CT sequence image to obtain a cavity volume in the seed and a total volume of the seed, wherein the volume difference between the total volume and the cavity volume is the effective volume of the seed;
the method comprises the following steps of obtaining the density of a kernel embryo and obtaining the density of kernel endosperm, wherein the steps comprise: sampling the grains to obtain an embryo sample block and an endosperm sample block; calculating the volume and weight of the embryo sample piece and calculating the volume and weight of the endosperm sample piece; calculating the density of the embryo from the volume and weight of the embryo sample piece and the density of the endosperm from the volume and weight of the endosperm sample piece; wherein the density of the embryo sample block is the same as that of the kernel embryo, and the density of the endosperm sample block is the same as that of the kernel endosperm;
calculating the volume of the embryo sample piece and calculating the volume of the endosperm sample piece, comprising the steps of: respectively carrying out CT sequence scanning on the embryo sample block and the endosperm sample block to respectively obtain a CT sequence image of the embryo sample block and a CT sequence image of the endosperm sample block; and respectively carrying out three-dimensional threshold segmentation on the CT sequence image of the embryo sample block and the CT sequence image of the endosperm sample block to respectively obtain the volume of the embryo sample block and the volume of the endosperm sample block.
2. The method of measurement of claim 1, wherein the step of calculating a volume fraction of embryo and a volume fraction of endosperm in a kernel based on the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm comprises:
the volume of the embryo in the kernel is the ratio of the volume of the embryo in the kernel to the effective volume of the kernel;
the volume of endosperm in the kernel is the ratio of the volume of endosperm in the kernel to the effective volume of the kernel.
3. The method of claim 1, wherein the step of calculating the kernel embryo volume and kernel endosperm volume based on the kernel effective volume, kernel weight, kernel embryo density, and kernel endosperm density comprises:
calculating the volume of the kernel embryo and the volume of the kernel endosperm by the following formula:
V1+V2=V;
p1·V1+p2·V2=W;
wherein V1 is the volume of the kernel embryo, V2 is the volume of the kernel endosperm, V is the effective volume of the kernel, p1 is the density of the kernel embryo, p2 is the density of the kernel endosperm, and W is the weight of the kernel.
4. A device for measuring the content of a seed component, comprising:
the first acquisition unit is used for acquiring the effective volume and weight of the seeds;
the second acquisition unit is used for acquiring the density of the kernel embryo and acquiring the density of the kernel endosperm;
the first calculating unit is used for calculating the volume of the kernel embryo and the volume of the kernel endosperm according to the effective volume of the kernel, the weight of the kernel, the density of the kernel embryo and the density of the kernel endosperm;
a second calculating unit, configured to calculate a volume ratio of an embryo in a kernel and a volume ratio of an endosperm according to the effective volume of the kernel, the volume of the kernel embryo and the volume of the kernel endosperm;
wherein, the step of obtaining the effective volume of the kernel comprises: performing CT scanning on the grains to obtain CT sequence images of the grains; performing three-dimensional threshold segmentation on the CT sequence image to obtain a cavity volume in the seed and a total volume of the seed, wherein the volume difference between the total volume and the cavity volume is the effective volume of the seed;
the method comprises the following steps of obtaining the density of a kernel embryo and obtaining the density of kernel endosperm, wherein the steps comprise: sampling the grains to obtain an embryo sample block and an endosperm sample block; calculating the volume and weight of the embryo sample piece and calculating the volume and weight of the endosperm sample piece; calculating the density of the embryo from the volume and weight of the embryo sample piece and the density of the endosperm from the volume and weight of the endosperm sample piece;
wherein the density of the embryo sample block is the same as that of the kernel embryo, and the density of the endosperm sample block is the same as that of the kernel endosperm;
calculating the volume of the embryo sample piece and calculating the volume of the endosperm sample piece, comprising the steps of:
respectively carrying out CT sequence scanning on the embryo sample block and the endosperm sample block to respectively obtain a CT sequence image of the embryo sample block and a CT sequence image of the endosperm sample block; and respectively carrying out three-dimensional threshold segmentation on the CT sequence image of the embryo sample block and the CT sequence image of the endosperm sample block to respectively obtain the volume of the embryo sample block and the volume of the endosperm sample block.
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