CN108191996A - A kind of preparation method of araboxylan - Google Patents
A kind of preparation method of araboxylan Download PDFInfo
- Publication number
- CN108191996A CN108191996A CN201810166354.1A CN201810166354A CN108191996A CN 108191996 A CN108191996 A CN 108191996A CN 201810166354 A CN201810166354 A CN 201810166354A CN 108191996 A CN108191996 A CN 108191996A
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- Prior art keywords
- corn bran
- centrifugation
- araboxylan
- ultrasonic
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of novel preparation methods of araboxylan in corn bran, and the present invention provides a kind of methods that ultrasonic synergistic microwave radiation technology alkaline process prepares araboxylan in corn bran.The present invention obtains corn bran araboxylan using cornstarch processing byproduct corn bran as raw material, by the processing of ultrasonic synergistic microwave radiation technology alkaline process, centrifugation, adjusting pH, centrifugation, reduced pressure, alcohol precipitation, centrifugation, freeze-drying.The present invention uses the physics householder method of supersonic synergic microwave on the basis of alkaline process, makes full use of the high penetrating power of microwave and the high efficiency of heating surface and the cavitation of ultrasonic wave, largely improves polysaccharide yield, shorten the production cycle；In addition the by-product that raw material of the invention is processed from starch, realizes waste utilization, and largely increase economic benefit.Araboxylan prepared by the present invention has certain antioxidation activity in vitro, is expected to develop into the food additives with preferable antioxidant activity.
Description
Technical field
The present invention relates to technical field of plant extraction, in more particularly to a kind of corn bran araboxylan prepare it is new
Method.
Background technology
Corn bran in the present invention is the by-product of cornstarch processing, and most corn brans are as feed or give up
Gurry, value are not developed and used fully, cause the great wasting of resources and environmental pollution.
Corn bran is a plant fiber raw material, containing several functions composition, such as：Cellulose, hemicellulose, phenol
Sour and a small amount of residual starch, wherein the araboxylan as hemicellulose main component（AX）Rich content.AX is to plant
Non-starch polysaccharide in object cell wall can be divided into soluble arabinoxylan according to its dissolubility（WEAX）And water-insoluble
Araboxylan（WUAX）.AX in cereal bran has many bioactivity, can be used as food additives, such as thicken
Agent, stabilizer, emulsifier etc. also have the characteristics such as immunological regulation, prebiotic, anti-oxidant and anti-inflammatory, antitumor, have caused at this stage
Extensive concern.
The research of AX is mainly concentrated in the cereal crops such as wheat, oat, barley, rice, sorghum both at home and abroad at present, wherein
With the AX content highests in corn bran, wheat bran.And according to the literature, araboxylan A/X exists in corn bran
0.667 or so, and come from the araboxylan A/X in wheat bran between 0.37-0.93, therefore in corn bran
Araboxylan is a kind of more unique polysaccharide.The extracting method of AX mainly has：Chemical-solvent method（Water extraction, enzyme formulation,
Sour formulation and alkaline extraction etc.）, physics auxiliary law（Steam explosion, extrusion, supercritical ultrasonics technology, microwave method etc.）.Wherein water extraction
Relatively low with enzyme formulation yield, acid system by-product is more and pollutes environment；Steam explosion condition is difficult to control, and extrusion operation is multiple
It is miscellaneous；Because the raw material of the present invention comes from the by-product of starch processing, the water-soluble araboxylan during processing
It has been be lost in that, therefore using the higher alkaline process of yield, assist WUAX being changed into WEAX by ultrasonic-microwave, reach raising polysaccharide
The purpose of yield.
Invention content
In view of the deficiencies of the prior art, the present invention provides a kind of ultrasonic synergistic microwave radiation technology alkaline process to prepare me
The method of primary xylan, this method are suitable for being factory produced.
(1) new method of the present invention for a kind of extraction of corn bran araboxylan, it is as follows that the method comprising the steps of：
（2）Sample treatment：105 DEG C of corn bran is dried 2 hours, and it is spare to crushed 40 mesh sieve.
（3）Sample pretreatment：Quantitative corn bran powder is weighed, adds in appropriate sodium hydroxide solution, ultrasonic wave and microwave association
With work 5min, reach certain temperature.
（4）Extraction process：Ultrasonic synergistic microwave radiation technology alkalinity extraction certain time, centrifugation carry out separation of solid and liquid, collect
Supernatant.
（5）Leaching liquor single treatment：Supernatant adjusts pH, and supernatant is collected by centrifugation again.
（6）Leaching liquor after-treatment：Supernatant is concentrated under reduced pressure, and absolute ethyl alcohol adjusts solution concentration, centrifuging and taking precipitation, freezing
Dry corn bran araboxylan.
（7）It is above-mentioned（3）Described in temperature condition for 70 DEG C, concentration of lye 0.25-0.35mol/L.
（8）It is above-mentioned（4）Described in extraction time be 20-30min, ultrasonic power 400-600W, centrifuge 4000r/min,
20min。
（9）It is above-mentioned（5）Described in pH for 4.5, centrifuge 4000r/min, 20min.
（10）It is above-mentioned（6）Described in ethyl alcohol adjust solution concentration be 65%, centrifuge 4000r/min, 10min.
The invention has the advantages that：
The raw material of the present invention come from cornstarch processing by-product --- corn bran derives from a wealth of sources, cheap, it is real
Waste utilization is showed, has reduced environmental pollution, and increased economic efficiency to the full extent and utility value；Present invention operation letter
It is single, it is easy to accomplish, using supersonic synergic microwave radiation technology alkalinity extraction corn bran araboxylan, largely improve
The yield of araboxylan, polysaccharide yield are up to 27.78%, more than 95% extraction rate reached to, hence it is evident that the change single higher than tradition
Learn solvent extraction method or single physics auxiliary law.
Description of the drawings
Fig. 1 is the process flow chart of ultrasonic synergistic microwave radiation technology alkalinity extraction corn bran araboxylan.
Specific embodiment
Embodiment one：
5g corn bran powder is taken, 165mL 0.25mol/L NaOH solutions configuration suspension is added in, is placed in supersonic synergic microwave dress
In putting, ultrasonic power 400W pre-processes 5min, elevates the temperature to 70 DEG C, the constant microwave power of supersonic synergic is handled
20min, centrifugation（4000r/min, 20min）, supernatant adjustment pH is 4.5, centrifugation（4000r/min, 20min）, supernatant subtracts
Pressure is concentrated into 1/4, and absolute ethyl alcohol adjustment solution concentration is 65%, the drying of centrifuging and taking pellet frozen, and it is poly- to obtain the Arabic wood of corn bran
Sugar.
Embodiment two：
5g corn bran powder is taken, 165mL 0.30mol/L NaOH solutions configuration suspension is added in, is placed in supersonic synergic microwave dress
In putting, ultrasonic power 500W pre-processes 5min, elevates the temperature to 70 DEG C, the constant microwave power of supersonic synergic is handled
25min, centrifugation（4000r/min, 20min）, supernatant adjustment pH is 4.5, centrifugation（4000r/min, 20min）, supernatant subtracts
Pressure is concentrated into 1/4, and absolute ethyl alcohol adjustment solution concentration is 65%, the drying of centrifuging and taking pellet frozen, and it is poly- to obtain the Arabic wood of corn bran
Sugar.
Embodiment three：
5g corn bran powder is taken, 165mL 0.35mol/L NaOH solutions configuration suspension is added in, is placed in supersonic synergic microwave dress
In putting, ultrasonic power 600W pre-processes 5min, elevates the temperature to 70 DEG C, the constant microwave power of supersonic synergic is handled
30min, centrifugation（4000r/min, 20min）, supernatant adjustment pH is 4.5, centrifugation（4000r/min, 20min）, supernatant subtracts
Pressure is concentrated into 1/4, and absolute ethyl alcohol adjustment solution concentration is 65%, the drying of centrifuging and taking pellet frozen, and it is poly- to obtain the Arabic wood of corn bran
Sugar.
Claims (9)
1. a kind of method that ultrasonic synergistic microwave radiation technology alkaline process prepares corn bran araboxylan, it is characterised in that：Institute
State raw material be cornstarch processing by-product corn bran, preparation method be ultrasonic synergistic microwave radiation technology alkaline process, then through from
The heart, adjust pH, centrifuge, be concentrated under reduced pressure again, alcohol precipitation, centrifugation, freeze-drying be made, specific preparation process is as follows：
(1) pretreatment of raw material：105 DEG C of corn bran is dried 2 hours, and it is spare to crushed 40 mesh sieve.
2.（2）Extraction pretreatment：Quantitative corn bran powder is weighed, adds in appropriate sodium hydroxide solution, ultrasonic wave and microwave cooperating
5min is pre-processed, reaches certain temperature.
3.（3）Extraction process：Ultrasonic synergistic microwave radiation technology alkalinity extraction certain time centrifuges and carries out separation of solid and liquid, in collection
Clear liquid.
4.（4）It will be above-mentioned（3）In supernatant be adjusted to proper pH value, supernatant is collected by centrifugation again.
5.（5）It will be above-mentioned（4）In supernatant be concentrated under reduced pressure into 1/4, ethyl alcohol adjusts appropriate solution concentration, centrifugation, and sediment is cold
It is lyophilized dry.
6. according to the method described in claim 1, it is characterized in that：Step（2）Described in temperature be 70 DEG C, concentration of lye is
0.25-0.35mol/L。
7. according to the method described in claim 1, it is characterized in that：Step（3）Described in the time be 20-30min, ultrasonic wave work(
Rate is 400-600W, centrifuges 4000r/min, 20min.
8. according to the method described in claim 1, it is characterized in that：Step（4）Described in pH value for 4.5, centrifuge 4000r/
Min, 20min.
9. according to the method described in claim 1, it is characterized in that：Step（5）In appropriate solution concentration for 65%, centrifugation
4000r/min, 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810166354.1A CN108191996A (en) | 2018-02-28 | 2018-02-28 | A kind of preparation method of araboxylan |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810166354.1A CN108191996A (en) | 2018-02-28 | 2018-02-28 | A kind of preparation method of araboxylan |
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| CN108191996A true CN108191996A (en) | 2018-06-22 |
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| CN201810166354.1A Pending CN108191996A (en) | 2018-02-28 | 2018-02-28 | A kind of preparation method of araboxylan |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109180839A (en) * | 2018-09-25 | 2019-01-11 | 山东百龙创园生物科技股份有限公司 | A kind of preparation method of araboxylan |
| CN110684124A (en) * | 2019-10-26 | 2020-01-14 | 桂林理工大学 | Preparation method and application of carboxymethylated xylan |
| CN113647609A (en) * | 2021-08-23 | 2021-11-16 | 河南科拓生物科技有限公司 | Compound emulsifying thickener |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045658A (en) * | 2012-12-24 | 2013-04-17 | 保龄宝生物股份有限公司 | Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid |
| CN104264262A (en) * | 2014-09-01 | 2015-01-07 | 郸城财鑫糖业有限责任公司 | Method for extracting araboxylan and protein fiber from wheat bran |
-
2018
- 2018-02-28 CN CN201810166354.1A patent/CN108191996A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045658A (en) * | 2012-12-24 | 2013-04-17 | 保龄宝生物股份有限公司 | Method of taking bran as raw materials to prepare high-purity low-poly araboxylan and ferulic acid |
| CN104264262A (en) * | 2014-09-01 | 2015-01-07 | 郸城财鑫糖业有限责任公司 | Method for extracting araboxylan and protein fiber from wheat bran |
Non-Patent Citations (9)
| Title |
|---|
| TOMOKI YOSHIDA等: ""Optimization of microwave-assisted extraction of carbohydrates from industrial waste of corn starch production using response surface methodology"", 《BIORESOURCE TECHNOLOGY》 * |
| YUYING JIANG等: ""Optimization of ultrasonic-microwave assisted alkali extraction of arabinoxylan from the corn bran using response surface methodology"", 《INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES》 * |
| 张莉莉等: ""超声-微波协同法水解玉米皮渣制备还原糖的工艺研究"", 《食品工业科技》 * |
| 林妲等: ""半纤维素分离纯化研究进展"", 《中国造纸》 * |
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| 范玲等: ""微波-超声波辅助提取小麦麸皮中酚基木聚糖的研究"", 《河南工业大学学报(自然科学版)》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109180839A (en) * | 2018-09-25 | 2019-01-11 | 山东百龙创园生物科技股份有限公司 | A kind of preparation method of araboxylan |
| CN110684124A (en) * | 2019-10-26 | 2020-01-14 | 桂林理工大学 | Preparation method and application of carboxymethylated xylan |
| CN113647609A (en) * | 2021-08-23 | 2021-11-16 | 河南科拓生物科技有限公司 | Compound emulsifying thickener |
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Application publication date: 20180622 |