CN108186601B - Sericin microcapsule and one-step preparation method - Google Patents
Sericin microcapsule and one-step preparation method Download PDFInfo
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- CN108186601B CN108186601B CN201810044872.6A CN201810044872A CN108186601B CN 108186601 B CN108186601 B CN 108186601B CN 201810044872 A CN201810044872 A CN 201810044872A CN 108186601 B CN108186601 B CN 108186601B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5021—Organic macromolecular compounds
- A61K9/5052—Proteins, e.g. albumin
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- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
- B01J13/02—Making microcapsules or microballoons
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a sericin microcapsule and a one-step preparation method, wherein the preparation method comprises the steps of mixing sericin powder and 1, 3-butanediol to ensure that the concentration of the sericin powder is 1-100mg/mL, keeping the temperature of 120 ℃ for 15-120min under the stirring condition, and washing with water to obtain the sericin microcapsule. The sericin microcapsule is a hollow microcapsule, is synthesized by a one-step method, does not need a cross-linking agent, and can load biomacromolecule medicines or other functional components. The preparation process has strong universality, easy operation, time and energy saving, is suitable for large-scale production, and the used materials have good biocompatibility and biodegradability. The invention is more suitable for application in the fields of functional component carriers, targeted delivery and skin care and beauty.
Description
Technical Field
The invention belongs to the field of medicine and beauty and skin care, and particularly relates to a one-step preparation method of a sericin microcapsule.
Background
Fibroin has a wide source, excellent price, excellent biocompatibility and biodegradability, and is receiving more and more attention in the fields of tissue engineering, regenerative medicine and drug delivery. Sericin is one of fibroin, has various functional groups, is favorable for combining with charged molecules, and can be used for preparing various drug carriers (Lamboni, L.; Gauthier, M.; Yang, G.; Wang, Q.; Biotechnol. adv.2015,33(8),1855 and 1867.). In addition, studies have shown that sericin exhibits active oxygen scavenging, anti-tyrosinase, anti-elastase and immunomodulatory activities and is expected to be used in cosmetics and dermatological products for anti-aging and anti-dermatitis (Chlapanidas, T.; Farag Och, S.; Lucconi, G.; Perteghella, S.; Galuzzi, M.; Mantelli, M.; Avanzini, M.A.; Tosca, M.C.; Marazzi, M.; Vigo, D.; Torre M.L.; Faustini, M., int.J.biol. Macromol.2013,58(Supplement C), 47-56). Sericin gel can reduce skin electrical impedance, increase hydroxyproline level and improve smoothness of upper skin, and is a good humectant (Padamwar, M.N.; Pawar, A.P.; Daithankar, A.V.; Mahadik, K.R., J.Cosmet.Dermatol.2005,4(4), 250-. Based on the advantages, the sericin has wide application in the pharmaceutical, biomedical, cosmetic and food industries.
The hollow structure carrier has wide application in the fields of drug delivery, bioreactors, biosensors and the like. Among them, microcapsule-based carriers are receiving increasing attention (ZL 201410066374.3; Du, B.; Wang, J.; Zhou, Z.; Tang, H.; Li, X.; Liu, Y.; Zhang, Q.Chem.Commun.,2014,50, 4423-4426.). Although the drug carrier based on sericin has various forms such as micro-nano particles, films, gels and the like, the research on sericin microcapsules is relatively less. Liu et al takes sericin protein solution and CaCl in a certain proportion2The solution was mixed, pH adjusted to 7.4, and incubated in a 37 ℃ incubator for 24 hours to prepare sericin microcapsules of different shapes for drug carriers (Liu, Z.; Cai, Y.; Jia, Y.; Liu, L.; Kong, X.; Kundu, S.C.; Yao, J., Macromol. Rapid Commun.2014,35(19), 8-Straus 1672, Yang, Y.; Cai, Y.; Sun, N.; Li, R.; Li, W.; Kundu, S.C.; Kong, X.; Yao, J., Colloids surf. BBioiface 2017,151(Supplement C), 102-Straus 111.), however, the preparation process took a long time. Despite the variety of methods used to prepare protein microcapsules, efficient, rapid, environmentally friendly preparation remains extremely challenging.
1, 3-butanediol has good moisture retention and certain antibacterial property, is commonly used in pharmaceutical preparations and cosmetics, and mainly comprises a topical pharmaceutical preparation, a.ito, a.a.; Masui, y.; Ito, m.j.dermaltol.2014, 41(9),815-816 and the like. The application of the microcapsule carrier in the preparation of microcapsule carriers has not been reported.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a sericin microcapsule.
The second purpose of the invention is to provide a one-step preparation method of the sericin microcapsule.
The third purpose of the invention is to provide an application of the sericin microcapsule.
The technical scheme of the invention is summarized as follows:
a one-step preparation method of sericin microcapsules is characterized by comprising the following steps:
mixing sericin powder with 1, 3-butanediol to make the concentration of sericin powder 1-100mg/mL, stirring, maintaining at 120 deg.C for 15-120min, and washing with water to obtain sericin microcapsule.
The sericin microcapsule prepared by the method.
The sericin microcapsule is applied to the preparation of the microcapsule carrying the functional components.
The functional component is protein or polysaccharide.
The sericin microcapsule is a hollow microcapsule, is synthesized by a one-step method, does not need a cross-linking agent, and can load biomacromolecule medicines or other functional components. The preparation process has strong universality, easy operation, time and energy saving, is suitable for large-scale production, and the used materials have good biocompatibility and biodegradability. The invention is more suitable for application in the fields of functional component carriers, targeted delivery and skin care and beauty.
Drawings
Fig. 1 is a light microscope photograph of the sericin microcapsule prepared in example 1.
FIG. 2 is a confocal microscope photograph of the sericin microcapsules prepared in example 1 in a FITC-dextran 2000KDa solution.
FIG. 3 is a confocal microscope photograph of the sericin microcapsules prepared in example 1 in a FITC-BSA solution.
Fig. 4 is a photo of the sericin microcapsule prepared in example 4.
Fig. 5 is a photo of the sericin microcapsule prepared in example 5.
Detailed Description
The present invention is further described with reference to the following specific examples, which are provided to enable those skilled in the art to better understand the present invention, but are not intended to limit the present invention in any way.
Example 1
A one-step preparation method of sericin microcapsules comprises the following steps:
mixing 1g of sericin powder with 100mL of 1, 3-butanediol, keeping the temperature of 120 ℃ for 30min under the stirring condition, and washing the mixture for three times to obtain sericin microcapsule suspension.
The above sericin microcapsule suspension was dropped on a glass slide and photographed under a light mirror, as shown in FIG. 1.
Example 2
0.5mL of the sericin microcapsule suspension prepared in example 1 was put in a container, and 0.5mL of an aqueous solution of FITC-labeled dextran (dextran molecular weight 2000kDa, abbreviated as FITC-dextran 2000KDa) with a mass concentration of 2mg/mL was added thereto and mixed to obtain a mixed solution, and the mixed solution was photographed under a confocal microscope after 30min (see FIG. 2). The inner part of the microcapsule has a fluorescence signal with the same intensity as that of an external solution, and the glucan with the molecular weight of 2000kDa can enter the inner part of the microcapsule through the pores on the wall of the sericin microcapsule, and the obtained sericin microcapsule can be used for entrapping the glucan.
Example 3
0.5mL of the sericin microcapsule suspension prepared in example 1 was put in a container, 0.5mL of an aqueous solution of FITC-labeled bovine serum albumin (FITC-BSA) at a mass concentration of 2mg/mL was added, and mixed to obtain a mixed solution, and the mixed solution was photographed under a confocal microscope after 30min (see FIG. 3). Most of the microcapsules have fluorescence signals with the same intensity as that of an external solution, and it is known that BSA can enter the microcapsules through pores in the wall of the sericin microcapsules, and the obtained sericin microcapsules can be used for encapsulation of BSA.
In addition, since it is known in the art that dextran, which is a model drug, is a representative of polysaccharide and BSA, which is a model drug, is a representative of protein, the sericin microcapsules of the present invention can be used as carriers for polysaccharide drugs and protein drugs.
Example 4
A one-step preparation method of sericin microcapsules comprises the following steps:
mixing 30mg of sericin powder with 30mL of 1, 3-butanediol, keeping the temperature of 120 ℃ for 15min under the stirring condition, and washing the mixture for three times to obtain sericin microcapsule suspension.
The above sericin microcapsule suspension was dropped on a glass plate and photographed under a light mirror, as shown in FIG. 4, and its morphology was similar to that of example 1.
Example 5
A one-step preparation method of sericin microcapsules comprises the following steps:
mixing 1g of sericin powder with 100mL of 1, 3-butanediol, keeping the temperature at 120 ℃ for 120min under the stirring condition, and washing with water for three times to obtain a sericin microcapsule suspension.
The above sericin microcapsule suspension was dropped on a glass plate and photographed under a light mirror, as shown in FIG. 5, and its morphology was similar to that of example 1.
Example 6
A one-step preparation method of sericin microcapsules comprises the following steps:
mixing 1g of sericin powder with 10mL of 1, 3-butanediol, keeping the temperature of 120 ℃ for 30min under the stirring condition, and washing the mixture for three times to obtain sericin microcapsule suspension.
The above sericin microcapsule suspension was dropped on a glass plate and photographed under a light microscope, and the morphology thereof was similar to that of example 1.
Claims (1)
1. A one-step preparation method of sericin microcapsules is characterized by comprising the following steps:
mixing sericin powder with 1, 3-butanediol to make the concentration of sericin powder 1-100mg/mL, stirring, maintaining at 120 deg.C for 15-120min, and washing with water to obtain sericin microcapsule.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810044872.6A CN108186601B (en) | 2018-01-17 | 2018-01-17 | Sericin microcapsule and one-step preparation method |
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| CN201810044872.6A CN108186601B (en) | 2018-01-17 | 2018-01-17 | Sericin microcapsule and one-step preparation method |
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| CN108186601B true CN108186601B (en) | 2020-09-01 |
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| CN110664777A (en) | 2019-10-11 | 2020-01-10 | 杭州协合医疗用品有限公司 | Sericin particle with oxidative stress property and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816842A (en) * | 2014-01-16 | 2014-05-28 | 浙江理工大学 | Sericin sphere microcapsule preparation method |
| CN104826563A (en) * | 2015-04-01 | 2015-08-12 | 浙江理工大学 | Preparation method of hydroxyapatite/sericin hybrid microcapsule |
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2018
- 2018-01-17 CN CN201810044872.6A patent/CN108186601B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103816842A (en) * | 2014-01-16 | 2014-05-28 | 浙江理工大学 | Sericin sphere microcapsule preparation method |
| CN104826563A (en) * | 2015-04-01 | 2015-08-12 | 浙江理工大学 | Preparation method of hydroxyapatite/sericin hybrid microcapsule |
Non-Patent Citations (2)
| Title |
|---|
| "结构仿生型丝胶蛋白微胶囊的制备及其应用研究";刘兆刚;《中国优秀硕士学位论文全文数据库（电子期刊）》;20140815(第8期);第9页倒数第2段，第11页倒数第2段，第15页"3 pH 对微胶囊形貌的影响"，第41页第2段 * |
| One-Step Synthesis of Natural Silk Sericin-Based Microcapsules with Bionic Structures;Zhaogang Liu et al.;《Macromolecular Rapid Communications》;20141231;第35卷;第1668？1672页 * |
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