CN108186133B - Method for positioning non-human primate brain substantia nigra region - Google Patents
Method for positioning non-human primate brain substantia nigra region Download PDFInfo
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- CN108186133B CN108186133B CN201711270468.2A CN201711270468A CN108186133B CN 108186133 B CN108186133 B CN 108186133B CN 201711270468 A CN201711270468 A CN 201711270468A CN 108186133 B CN108186133 B CN 108186133B
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Abstract
The invention discloses a method for positioning a brain substantia nigra region of a non-human primate. The method utilizes the nuclear magnetic resonance photograph to obtain the positioning coordinates of the brain substantia nigra area of the non-human primate, can realize the accurate positioning of the brain substantia nigra area of the non-human primate, and has the advantages of higher accuracy and reliability, high efficiency and experiment cost saving compared with the existing method for carrying out extensive positioning according to experience.
Description
Technical Field
The invention belongs to the technical field of animal brain structure positioning, and particularly relates to a method for obtaining positioning coordinates of a non-human primate brain substantia nigra region by utilizing a nuclear magnetic resonance photograph so as to realize accurate positioning of the non-human primate brain substantia nigra region.
Background
The accurate injection technology of the substantia nigra region of the brain is a key technology for developing a Parkinson disease model and drug evaluation research. In rodents (rats and mice), viral or non-viral vectors can be accurately and locally injected into the substantia nigra region of the brain through brain mapping and brain stereotaxic mapping, so that an animal model with the clinical pathological characteristics of Parkinson can be successfully constructed, and meanwhile, the technology can also be used for research and evaluation of Parkinson Gene Therapy and stem cell Therapy (see "Gene Therapy for Neurological disorders methods and Protocols" clinical P. manfredsson edition "). The brain pathophysiology process of the non-human primate is highly similar to that of human, and is an optimal animal model for researching and developing brain related diseases, the development condition difference between experimental animals of the non-human primate is large, and the skull and frontal face morphology of animals with the same age and sex are different, so that the brain substantia nigra region is still difficult to realize positioning operation by combining a brain positioning map with a three-dimensional positioning method.
Disclosure of Invention
The invention aims to: the method solves the problem that the individual difference of the non-human primate is large so that the brain substantia nigra area is difficult to accurately position in the prior art, and provides a method for obtaining the positioning coordinates of the brain substantia nigra area of the non-human primate by using nuclear magnetic resonance photographs so as to realize the accurate positioning of the brain substantia nigra area of the non-human primate.
In order to achieve the above object, the present invention provides a method for localizing a brain substantia nigra region of a non-human primate, comprising the steps of:
s1, the non-human primate is laid on the nuclear magnetic resonance machine, the face of the non-human primate faces to the right upper side, and nuclear magnetic resonance scanning is started to obtain a nuclear magnetic resonance picture;
s2, opening a nuclear magnetic resonance photo by using RadiAnt DICOM Viewer software, selecting a photo in the middle of a sagittal plane of a brain, drawing a reference line between the center of the eyebrow and an inner protuberance of the occipital (proved by multiple experiments, the reference line is a horizontal line when an animal is placed in a brain stereotaxic apparatus), after determining a black area on the nuclear magnetic resonance photo, perpendicular to the reference line, taking the distance between the dura mater and the black area of the brain as a Y coordinate, and taking the distance between the center of the eyebrow and the Y coordinate as a Z coordinate;
s3, selecting a brain cross section photo, drawing a reference line between the eyebrow center and the occipital protuberance (multiple experiments prove that the reference line is parallel to a sagittal suture of a skull, the plane formed by the two lines is a median sagittal plane of the brain, and the reference line can be used as a brain midline), determining a substantia nigra region on the nuclear magnetic resonance photo, and then, perpendicular to the reference line, taking the distance between the reference line and the substantia nigra region of the brain as an X coordinate;
and S4, positioning the non-human primate brain substantia nigra region according to the X coordinate, the Y coordinate and the Z coordinate.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention mainly solves the key technical problem of how to accurately position the brain substantia nigra region of the non-human primate, accurately digitalizes and coordinates the position of the brain substantia nigra region in the magnetic resonance picture, and simultaneously removes the influence on the positioning of the brain substantia nigra region due to the placement difference of the monkey brain because of the difference between the placement of the brain of the magnetic resonance picture and the placement of the brain on a brain stereotaxic apparatus in actual operation, thereby realizing the accurate positioning of the brain substantia nigra region of the non-human primate.
(2) The method utilizes the magnetic resonance photograph to obtain the positioning coordinates of the brain substantia nigra area of the non-human primate, and can accurately position and inject the brain substantia nigra area of the non-human primate by combining with the brain stereotactic positioning.
Drawings
Fig. 1 is a median picture of a monkey magnetic resonance sagittal plane.
Fig. 2 is a median picture of a magnetic resonance sagittal plane of a monkey, and a reference line between the glabellum and the inion is taken as a horizontal line of the monkey on a cerebral positioning instrument.
Fig. 3 is a schematic diagram of the location of the substantia nigra region of the brain (black box).
FIG. 4 is a graph showing the determination of the perpendicular distance (Y-coordinate) of the dura to the substantia nigra region (indicated by the arrow) of the brain, based on a reference line determined perpendicular to that of FIG. 2.
Fig. 5 is a central picture of a monkey magnetic resonance sagittal plane, in which the vertical distance (Z-coordinate) from the center of the eyebrow to the Y-coordinate determined in fig. 4 is determined.
FIG. 6 is a schematic diagram of the substantia nigra region of a cross section of a brain.
Fig. 7 is a monkey magnetic resonance brain cross-sectional image.
Fig. 8 is a magnetic resonance brain cross-section picture of a monkey, a reference line between the glabellum and the inion is parallel to a sagittal suture of the skull of the monkey, a plane formed by the two lines is a median sagittal plane of the brain, and the reference line can be used as a brain midline.
Fig. 9 is a graph showing the determination of the horizontal distance (X-coordinate) from the brain substantia nigra region (indicated by an arrow) to the brain midline based on the reference line determined in fig. 8.
FIG. 10 shows the results of using the method of the present invention to locate the substantia nigra region in the brain of monkey # 1.
FIG. 11 shows the results of using the method of the present invention to locate the substantia nigra region in the brain in monkey # 2.
FIG. 12 shows the results of using the method of the present invention to locate the substantia nigra region in the brain in monkey # 3.
Detailed Description
In order to make the objects, technical solutions and advantageous technical effects of the present invention clearer, the present invention is further described in detail with reference to the following embodiments. It should be understood that the embodiments described in this specification are only for the purpose of illustrating the invention and are not to be construed as limiting the invention, and the parameters, proportions and the like of the embodiments may be suitably selected without materially affecting the results.
Unless otherwise specified, reagents and materials used in the following examples are commercially available.
Examples
1) After the monkey is anesthetized, the monkey is placed on a nuclear magnetic resonance machine in a lying mode, the head of the monkey is lifted by a cushion towel, the face of the monkey faces upwards, and then nuclear magnetic resonance scanning can be started.
2) The acquired nuclear magnetic resonance photo is opened by utilizing the RadiAnt DICOM Viewer software, the photo in the middle of the sagittal plane of the monkey brain is selected (as shown in figure 1), a measuring tool of the RadiAnt DICOM Viewer software is selected, a datum line (as shown in figure 2) is pulled through the eyebrow center (as shown in the left arrow of figure 2) and the occipital protuberance (as shown in the right arrow of figure 2) of the monkey, and multiple experiments prove that the datum line is a horizontal line of the monkey placed behind the brain stereotaxic locator. From the location of the substantia nigra region in the anatomical atlas (fig. 3), perpendicular to the baseline, the distance from the parietal dura mater to the substantia nigra region (fig. 4 arrow) is determined (see fig. 4 longitudinal line), which is the Y coordinate. Perpendicular to the Y-coordinate, the distance from the center of the eyebrow (i.e., the point of the baseline eyebrow) to the Y-coordinate is measured (see fig. 5), which is the Z-coordinate.
3) The acquired nuclear magnetic resonance photo is opened by utilizing the RadiAnt DICOM Viewer software, a picture which can show the largest brain substantia nigra region (shown in figure 6) in the monkey brain cross-section photo is selected, as shown in the nuclear magnetic picture of figure 7, a measuring tool of the RadiAnt DICOM Viewer software is selected, a datum line is drawn through the eyebrow center (shown in the arrow at the upper part of figure 8) and the occipital protuberance (shown in the arrow at the bottom of figure 8) of the monkey, the datum line is parallel to the sagittal suture of the skull, the plane formed by the two lines is the median sagittal plane of the brain, the datum line can be used as the midline (shown in figure 8) of the brain, the distance from the sagittal suture of the skull to the substantia nigra region (shown in the line corresponding to 3.54 in figure 9) is determined, and the distance is the X coordinate, and the brain substantia nigr.
Examples 1 to 3
According to the positioning method provided by the embodiment of the invention, a single-side substantia nigra region injection glycerol test is carried out on part of monkeys (glycerol diffuses slowly, so that magnetic resonance is conveniently utilized again for scanning confirmation after injection, and in actual use, injected liquid diffuses quickly and the injection volume is small, so that magnetic resonance cannot be used for scanning confirmation).
1) The monkey No. 1 utilizes the embodiment positioning method to determine X (3.98 mm from a brain midline), Y (2.91 cm from dura mater when a substantia nigra region is perpendicular to a horizontal line) and Z coordinates (5.06 cm from an eyebrow center to a Y axis) after magnetic resonance scanning is carried out on the monkey brain, positioning injection of glycerin is carried out on the substantia nigra region on a brain stereotaxic apparatus after the monkey is anesthetized according to the positioning method, and white liquid in the substantia nigra region can be clearly seen through magnetic resonance scanning again after injection (see arrow in figure 10).
2) The monkey 2 uses the embodiment positioning method to perform magnetic resonance scanning on the monkey brain, then determines X (3.34 mm from the brain midline), Y (2.75 cm from dura mater perpendicular to the horizontal line), and Z coordinate (5.31 cm from Y axis perpendicular to the glabellar), anesthetizes the monkey according to the positioning method, performs positioning injection of glycerol on the substantia nigra region on the brain stereotaxic apparatus, and can clearly see that white liquid appears in the substantia nigra region of the brain (see arrow in fig. 11) through magnetic resonance scanning after injection.
3) The monkey 3 uses the embodiment positioning method to perform magnetic resonance scanning on the monkey brain, then determines X (2.36 mm from the brain midline), Y (2.81 cm from the dura mater when the substantia nigra region is perpendicular to the horizontal line) and Z coordinates (4.69 cm from the glabella perpendicular to the Y axis), performs positioning injection of glycerin on the substantia nigra region on a brain stereotaxic apparatus after anesthesia of the monkey according to the positioning method, and can clearly see that white liquid appears in the substantia nigra region of the brain (see an arrow in fig. 12) through magnetic resonance scanning again after injection.
Appropriate changes and modifications to the embodiments described above will become apparent to those skilled in the art from the disclosure and teachings of the foregoing description. Therefore, the present invention is not limited to the specific embodiments disclosed and described above, and some modifications and variations of the present invention should fall within the scope of the claims of the present invention. Furthermore, although specific terms are employed herein, they are used in a generic and descriptive sense only and not for purposes of limitation.
Claims (1)
1. The method for positioning the substantia nigra region of the brain of the non-human primate is characterized by comprising the following steps of:
s1, the non-human primate is laid on the nuclear magnetic resonance machine, the face of the non-human primate faces to the right upper side, and nuclear magnetic resonance scanning is started to obtain a nuclear magnetic resonance picture;
s2, opening a nuclear magnetic resonance photo by using RadiAnt DICOM Viewer software, selecting a photo in the middle of a sagittal plane of a brain, drawing a reference line between an eyebrow center and an occipital protuberance, wherein the reference line is a horizontal line of a monkey placed behind a brain stereotaxic apparatus, after determining a substantia nigra region on the nuclear magnetic resonance photo, the reference line is perpendicular to the nuclear magnetic resonance photo, the distance between a dura mater and the substantia nigra region of the brain is taken as a Y coordinate and is perpendicular to the Y coordinate, and the distance between the eyebrow center and the Y coordinate is measured and taken as a Z coordinate;
s3, selecting a brain cross section picture, drawing a reference line between the eyebrow center and the occipital protuberance, wherein the reference line is parallel to a sagittal suture of the skull, a plane formed by the two lines is a median sagittal plane of the brain, the reference line is used as a brain central line, after a substantia nigra region is determined on a nuclear magnetic resonance photograph, the reference line is perpendicular to the reference line, and the distance between the reference line and the substantia nigra region of the brain is taken as an X coordinate;
s4, positioning the non-human primate brain substantia nigra region according to the X coordinate, the Y coordinate and the Z coordinate;
and S5, accurately positioning the non-human primate brain substantia nigra region by combining with a brain stereotaxic apparatus.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011025836A1 (en) * | 2009-08-25 | 2011-03-03 | The Regents Of The University Of California | Optimized placement of cannula for delivery of therapeutics to the brain |
| CN103284980A (en) * | 2013-06-18 | 2013-09-11 | 中国科学院昆明动物研究所 | Method for establishing animal model for senile dementia, special liquid medicine and dosing device |
| CN103417538A (en) * | 2013-06-28 | 2013-12-04 | 云南中科灵长类生物医学重点实验室 | Dedicated liquid medicine, dedicated tool and method for building animal model of parkinson's disease through intracerebral administration |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011025836A1 (en) * | 2009-08-25 | 2011-03-03 | The Regents Of The University Of California | Optimized placement of cannula for delivery of therapeutics to the brain |
| CN103284980A (en) * | 2013-06-18 | 2013-09-11 | 中国科学院昆明动物研究所 | Method for establishing animal model for senile dementia, special liquid medicine and dosing device |
| CN103417538A (en) * | 2013-06-28 | 2013-12-04 | 云南中科灵长类生物医学重点实验室 | Dedicated liquid medicine, dedicated tool and method for building animal model of parkinson's disease through intracerebral administration |
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