CN108181143A - A kind of method of quick detection grease phosphorus content - Google Patents
A kind of method of quick detection grease phosphorus content Download PDFInfo
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- CN108181143A CN108181143A CN201711317742.7A CN201711317742A CN108181143A CN 108181143 A CN108181143 A CN 108181143A CN 201711317742 A CN201711317742 A CN 201711317742A CN 108181143 A CN108181143 A CN 108181143A
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- grease
- phosphorus content
- quick detection
- acetone
- extracting solution
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/03—Edible oils or edible fats
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
- G01N2001/4061—Solvent extraction
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- General Physics & Mathematics (AREA)
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
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- Biomedical Technology (AREA)
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention belongs to technical field of chemistry, disclose a kind of method of quick detection grease phosphorus content, including：Acetone is used to carry out grease phosphorous in oil and grease extracting, obtain extract liquor；The extract liquor is ultrasonically treated, obtains extracting solution；Reading is carried out by TL2300 to extracting solution, obtains phosphorus content concrete numerical value.Using the method for inspection provided by the invention it is efficient, loss is small, the rate of recovery is high, easy to operate, available for the inspection of process grease in production process and the inspection of batch grease.
Description
Technical field
The invention belongs to technical field of chemistry, and in particular to a kind of method of quick detection grease phosphorus content.
Technical background
Phosphatide is the main component for forming biomembrane, is divided into glycerophosphatide and two major class of sphingomyelins, respectively glycerine and sheath
Ammonia alcohol is formed.Phosphatide is amphiphatic molecule, and one end is hydrophilic nitrogenous or phosphorus head, and the other end is hydrophobic（Oleophylic）Long alkyl
Chain.Therefore, phospholipid molecule often collectively forms lipid bilayer with other molecules such as protein, glycolipid, cholesterol, i.e. cell membrane
Structure.Plant phosphatide is primarily present in oilseed, and is mostly present in gel phase, and with protein, carbohydrate, fat
The substances such as acid, mykol, vitamin exist with bonding state, are a kind of important grease accompaniments.Phosphatide is mainly derived from plant
Grease, as content of phospholipid is about 0.1%-1.8% in soybean oil crude oil.
Phosphatide is widely present in the cell tissue of oil crops, is the additive in food industry, in oilseed
Phosphatide enters with liquefaction process in oil product, although carrying out aquation dephosphorization treatment in oil refining process, since phosphatide has fat
Dissolubility and water-soluble two attribute, therefore be difficult that processing is thorough in refining process.Phosphatide heat resistance is poor, and when high temperature is easily carbonized, and makes
Grease overflows foam blackening, influences the edible quality of grease.Meanwhile when content of phospholipid is excessively high in oil product, grease storage-stable is also influenced
Property, and the content of phospholipid in crude oil is then to determine the foundation of processing capacity in grease hydration process, so, examine crude oil and product oil
In content of phospholipid, for grasp production operation and ensure oil quality be all indispensable.
Invention content
The object of the present invention is to provide a kind of method of quick detection grease phosphorus content, with inspection phosphorus provided by the present invention
The method for quickly detecting of content can significantly improve checkability, reduce loss amount, reduce inspection cost, improve inspection
Efficiency is suitble to plant processes monitoring and batch to examine.
It is of the invention main to realize by following technical solution：
A kind of method of quick detection grease phosphorus content, which is characterized in that the method for inspection includes the following steps：
Step（1）：Accurately grease is weighed to be placed in 50mL volumetric flasks；
Step（2）：Using the extracting process of acetone soak, acetone mixing is added in, obtains extracting solution；
Step（3）：By step（2）Obtained extracting solution, which is placed in supersonic cleaning machine, to be ultrasonically treated；
Step（4）：By step（3）Extracting solution after supersound process is cooled to room temperature, and is settled to scale；
Step（5）：By step（4）Extracting solution after cooling, immigration sample injection bottle are ready to pass through transmissometer and are analyzed.
Preferably,
The quality of the grease is 3.0 ± 0.1g.
Preferably,
The step（2）, using the phosphatide in acetone soak processing oil and grease extracting.
Preferably,
The step（3）In, using supersound process grease and acetone mixture, ultrasonic 30min, ultrasonic power 300-400W.
The application of the above-mentioned method for being allowed to a kind of quick detection grease phosphorus content described in one is also claimed in the present invention.
Compared with prior art, the present invention has but be not restricted to that advantages below：
（1）A kind of method for quickly detecting of grease phosphorus content provided by the invention avoids excessive reagent from turning from handling to analyzing
It moves, reduces the error that operation is brought to greatest extent.
（2）A kind of method for quickly detecting of grease phosphorus content provided by the invention, smashes grease using ultrasound, fills acetone
Divide the phospholipid composition in oil and grease extracting.
（3）The method for quickly detecting of a kind of grease phosphorus content provided by the invention, using in acetone soak extraction grease
Phosphatide, reduces the type that drug uses, and reduces harm of the drug to human body and environment as far as possible.
（4）A kind of method for quickly detecting of grease phosphorus content provided by the invention, the whole process of inspection only use one kind
Drug saves drug, and technological operation is simple, efficient low-consume, realizes making full use of for drug and equipment.
（5）A kind of method for quickly detecting of grease phosphorus content provided by the invention, the whole process used time of inspection is short, improves
Checkability is suitble to process monitoring and batch to examine.
（6）Using the technical solution of comparative example 1, accuracy declines more than 20%, and repeatability is greatly reduced, and illustrates ultrasound
Processing time is affected to testing result.
Specific embodiment
In order to make those skilled in the art better understand the technical solutions in the application, have below in conjunction with the application
Body embodiment more clearly and completely describes the present invention, it is clear that described embodiment is only the application one
Divide embodiment, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
All other embodiments obtained under the premise of creative work should all belong to the scope of protection of the invention.
Embodiment 1
A kind of method of quick detection grease phosphorus content, includes the following steps：
Step（1）：Accurately 3.0 ± 0.1g greases are weighed to be placed in 50mL volumetric flasks；
Step（2）：Using the extracting process of acetone soak, 30 ± 0.1mL acetone is accurately added in into volumetric flask, is extracted
Liquid；
Step（3）：By step（2）Obtained extracting solution, which is placed in supersonic cleaning machine, carries out ultrasound 30min, ultrasonic power 300-
400W.；
Step（4）：By step（3）Extracting solution after ultrasound is cooled to room temperature, and is settled to scale；
Step（5）：By step（4）Extracting solution after cooling, immigration sample injection bottle are ready to pass through TL2300 transmissometers and are analyzed.
Reference examples 1
A kind of method of quick detection grease phosphorus content, includes the following steps：
Step（1）：Accurately 3.0 ± 0.1g greases are weighed to be placed in 50mL volumetric flasks；
Step（2）：Using the extracting process of acetone soak, 30 ± 0.1mL acetone is accurately added in into volumetric flask, is extracted
Liquid；
Step（3）：By step（2）Obtained extracting solution, which is placed in supersonic cleaning machine, carries out ultrasound 1min, ultrasonic power 300-
400W；
Step（4）：By step（3）Extracting solution after ultrasound is cooled to room temperature, and is settled to scale；
Step（5）：By step（4）Extracting solution after cooling, immigration sample injection bottle are ready to pass through TL2300 transmissometers and are analyzed.
Embodiment 3
The experimental data comparative analysis of embodiment 1
By multiple parallel test, the reproducibility better result of testing result is shown in Table 1：
Table 1
Compare third party testing agency（Compose Buddhist nun）, the good lattice in Suzhou and delicious fresh testing result comparison be shown in Table 2：
Table 2
Conclusion：By multiple parallel test, show that the reproducibility of this method is preferable, point before the Rapid examination method comparison of phosphatide
Light photometer measuring method, result have differences, and belong to nominal error range.By comparing third party inspection center and other companies
Testing result, the result obtained by the fast method equally exists error, belongs in the range of nominal error.
This experiment detects the phosphorus content of grease by transmissometer using the phosphatide in acetone reagent extraction edible oil.Experiment
The result shows that：The test repeatability of this method is strong, is of little difference compared with spectrophotometry, but the processing time of sample is shorter,
Reagent type used is less, improves checkability, and plant processes monitoring and batch is suitble to examine.
Using the technical solution of comparative example 1, accuracy declines more than 20%, and reproducibility is greatly reduced, and illustrates to be ultrasonically treated
Time is affected to testing result.
It has also been found that acetone addition volume also has an impact testing result, acetone>During 30mL, detection error 5- less than normal
15%,<During 30mL, detection error 5-15% bigger than normal.Therefore, select suitable volume more important.
Exemplified as above is only a part of specific embodiment of the present invention.Instead of all the embodiments.Obviously, the present invention is unlimited
In above example, acceptable there are many deform.Based on the embodiment in the application, those of ordinary skill in the art are not doing
Go out all other embodiments obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Claims (6)
- A kind of 1. method of quick detection grease phosphorus content, which is characterized in that the method for inspection includes the following steps：Step（1）：Accurately grease is weighed to be placed in 50mL volumetric flasks；Step（2）：Using the extracting process of acetone soak, acetone mixing is added in, obtains extracting solution；Step（3）：By step（2）Obtained extracting solution, which is placed in supersonic cleaning machine, to be ultrasonically treated；Step（4）：By step（3）Extracting solution after supersound process is cooled to room temperature, and is settled to scale；Step（5）：By step（4）Extracting solution after cooling, immigration sample injection bottle are ready to pass through transmissometer and are analyzed.
- A kind of 2. method of quick detection grease phosphorus content according to claim 1, which is characterized in that the matter of the grease It measures as 3.0 ± 0.1g.
- A kind of 3. method of quick detection grease phosphorus content according to claim 1, which is characterized in that the step（2）, Using the phosphatide in acetone soak processing oil and grease extracting.
- A kind of 4. method of quick detection grease phosphorus content according to claim 1, which is characterized in that the step（3） In, using supersound process grease and acetone mixture, ultrasonic 30min, ultrasonic power 300-400W.
- A kind of 5. method of quick detection grease phosphorus content according to claim 1, which is characterized in that the body of the acetone Product is 30 ± 0.1mL.
- 6. a kind of application of the method for quick detection grease phosphorus content according to claim 1-5 is allowed to one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711317742.7A CN108181143A (en) | 2017-12-12 | 2017-12-12 | A kind of method of quick detection grease phosphorus content |
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| CN201711317742.7A CN108181143A (en) | 2017-12-12 | 2017-12-12 | A kind of method of quick detection grease phosphorus content |
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| CN108181143A true CN108181143A (en) | 2018-06-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110927004A (en) * | 2019-11-21 | 2020-03-27 | 中国农业科学院北京畜牧兽医研究所 | Fat analyzer |
| CN111458257A (en) * | 2020-04-03 | 2020-07-28 | 青岛天祥食品集团有限公司 | Method for detecting content of filter aid and phospholipid in pressed peanut oil filter residue |
Citations (4)
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|---|---|---|---|---|
| US5974899A (en) * | 1997-12-05 | 1999-11-02 | Hanks; Dallas A. | Soil nutrient extraction method using pressurized hot water |
| CN1974582A (en) * | 2006-12-01 | 2007-06-06 | 广州汉方现代中药研究开发有限公司 | Supersonically reinforced yolk phospholipid extracting process |
| CN101556241A (en) * | 2009-05-27 | 2009-10-14 | 谱尼测试科技（北京）有限公司 | Simple egg lecithin content measuring method |
| CN107247051A (en) * | 2017-08-14 | 2017-10-13 | 道道全重庆粮油有限责任公司 | A kind of method of quick detection phospholipid in lipid |
-
2017
- 2017-12-12 CN CN201711317742.7A patent/CN108181143A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5974899A (en) * | 1997-12-05 | 1999-11-02 | Hanks; Dallas A. | Soil nutrient extraction method using pressurized hot water |
| CN1974582A (en) * | 2006-12-01 | 2007-06-06 | 广州汉方现代中药研究开发有限公司 | Supersonically reinforced yolk phospholipid extracting process |
| CN101556241A (en) * | 2009-05-27 | 2009-10-14 | 谱尼测试科技（北京）有限公司 | Simple egg lecithin content measuring method |
| CN107247051A (en) * | 2017-08-14 | 2017-10-13 | 道道全重庆粮油有限责任公司 | A kind of method of quick detection phospholipid in lipid |
Non-Patent Citations (2)
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| 刘玲娜: "超声波辅助萃取菜籽磷脂酰胆碱的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110927004A (en) * | 2019-11-21 | 2020-03-27 | 中国农业科学院北京畜牧兽医研究所 | Fat analyzer |
| CN111458257A (en) * | 2020-04-03 | 2020-07-28 | 青岛天祥食品集团有限公司 | Method for detecting content of filter aid and phospholipid in pressed peanut oil filter residue |
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