CN108179222A - Chorismate mutase nucleotide sequence relevant with rice high yield and its application - Google Patents
Chorismate mutase nucleotide sequence relevant with rice high yield and its application Download PDFInfo
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Abstract
The invention discloses a kind of and the relevant chorismate mutase nucleotide sequence of rice high yield and its applications.The method of rice of the screening with different output character provided by the present invention, it may include following steps：(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located in rice genome, are OSCM3, there are two kinds of allelic forms of OSCM3_a and OSCM3_b, and the OSCM3_a is as shown in the sequence 1 of sequence table, and the OSCM3_b is as shown in the sequence 2 of sequence table；(2) judged as follows：Compare two genotype in the case where adjoining growth conditions, be that the average product of the homozygous Rice Populations of OSCM3_b is higher than the average product that genotype is the homozygous Rice Populations of OSCM3_a.It is demonstrated experimentally that method provided by the invention can Effective selection there is the rice of different single plant yield characters, and easy to operate, accuracy rate is high, has important application value in rice breeding.
Description
Technical field
The present invention relates to biotechnology more particularly to a kind of and relevant chorismate mutase nucleotide of rice high yield
Sequence and its application.
Background technology
With the growth of population in the world, the demand for cultivating high-yield crop is growing day by day.Rice (Oryza sativa L.) is
One of staple food source that majority rely on the earth.Finding in rice influences the genomic fragment of high yield, and establishes and effectively comment
Estimate specific method of the genomic fragment to yield effect, have important impetus to the development of molecular breeding.In breeding process
Targetedly choosing the rice varieties that parent material provides high-yield character to finally cultivating has important value.At present science and
The development of technology has been to obtain the natural variation of genome ingredient, deep exploitation paddy gene that high-yield character is influenced in rice
Provide possibility.
In practice, breeding method relies on visible character or/and molecular labeling mostly.Identification pair with yield related gene
It is confined to the means such as the analysis to mutant mostly at present.Since the factor for influencing rice yield traits is complex, Duo Zhongji
Influence of the effect and environment of cause to them may make influence of the effective evaluation genomic fragment to yield become difficult.Obviously
For yield, a restraining factors of molecular breeding are that effective experimental design is taken under field condition, so which is determined
A little genes or molecule fragment are suitable as alternative condition.With reference to rice in the performance in crop field and the quantitative study pair of its functional gene
Agricultural breeding can play directive function.
Invention content
The gene order for the present invention provides one being proved to that rice yield can be increased and its special special primer pair, and
Establishing an effective evaluation gene order as example influences the method for yield.
Screening technique provided by the present invention is suitably selected for the rice (method first) of high yield, includes the following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located at paddy gene
In group, there are two kinds of allelic forms of at least OSCM3_a and OSCM3_b, the OSCM3_a is described as shown in the sequence 1 of sequence table
OSCM3_b is as shown in the sequence 2 of sequence table；
(2) judged as follows：Under neighbouring growth conditions, the genotype Rice Population homozygous for OSCM3_b is put down
The average product of the equal yield Rice Population homozygous for OSCM3_a higher than genotype.
The neighbouring growth conditions is adjacent in same geographic area and equal growth conditions.
Screening technique provided by the present invention is suitably selected for the rice (method second) of high yield, it may include following steps：
(1) using the genomic DNA of rice to be measured as template, using following special primers to carrying out PCR amplification；If PCR
Amplified production only has one kind and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_a；If
Pcr amplification product only has one kind and as shown in sequence 2 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_b；
(2) judged as follows：Under neighbouring growth conditions, the genotype Rice Population homozygous for OSCM3_b is put down
The average product of the equal yield Rice Population homozygous for OSCM3_a higher than genotype.
The present invention can also provide a kind of screening technique of high-yield rice, be method A or method B；
The method A includes the following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located at paddy gene
In group, be OsCM3, there are two kinds of allelic forms of OsCM3_a and OsCM3_b, the OsCM3_a as shown in the sequence 1 of sequence table,
The OsCM3_b is as shown in the sequence 2 of sequence table；
(2) judged as follows：Under equal growth conditions, the genotype Rice Population homozygous for OsCM3_b is averaged
Yield is above the average product of the genotype Rice Population homozygous for OsCM3_a；
The method B includes the following steps：
(1) using the genomic DNA of rice to be measured as template, using special primer described in claim 4 to carrying out PCR expansions
Increase；If pcr amplification product only has one kind and as shown in sequence 1 in sequence table, the genotype of rice to be measured is pure for OsCM3_a
Mould assembly；If pcr amplification product only has one kind and as shown in sequence 2 in sequence table, the genotype of rice to be measured is OsCM3_b
It is homozygous；
(2) judged as follows：Under equal growth conditions, the genotype Rice Population homozygous for OsCM3_b is averaged
Yield is above the average product of the genotype Rice Population homozygous for OsCM3_a.
Above-mentioned equal growth conditions can be same geographical area equal growth conditions or different geographic regions it is equal
Growth conditions.
Growth conditions refers to natural environment (soil, temperature, humidity and nutrient etc.) and labor management (fertilising, Pesticide use, drive
Bird and drive pest and disease damage etc.).
The present invention also provides a kind of seed selection method for rice (methods the third), it may include following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located at paddy gene
In group, be OSCM3, there are two kinds of allelic forms of OSCM3_a and OSCM3_b, the OSCM3_a as shown in the sequence 1 of sequence table,
The OSCM3_b is as shown in the sequence 2 of sequence table；
(2) genotype is the purpose rice that the homozygous rice of OSCM3_b is selection and breeding.
The present invention also provides a kind of seed selection method for rice (method fourths), it may include following steps：
(1) using the genomic DNA of rice to be measured as template, using following special primers to carrying out PCR amplification；If PCR
Amplified production only has one kind and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_a；If
Pcr amplification product only has one kind and as shown in sequence 2 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_b；
(2) genotype is the purpose rice that the homozygous rice of OSCM3_b is selection and breeding.
The purpose rice rice high for yield.
The present invention also protects a kind of DNA fragmentation, is OsCM3_b；
The OsCM3_b can be following (d1) or (d2)：
(d1) DNA molecular shown in the sequence 2 of sequence table；
(d2) sequence 2 is passed through into the substitution of one or several nucleotide and/or lacks and ors add and have with sequence 2
The DNA molecular of identical function.
OsCM3_a genes and OsCM3_b genes are allele.
In above-mentioned, the position that " replace and/or lack and or add " occurs is located at shown in the sequence 1 of sequence table
The region other than difference between OsCM3_a genes and the sequence of sequence table 2.
The primer pair provided by the present invention for being used to expand above-mentioned DNA fragmentation, is made of primer 1 and primer 2；
The primer 1 can be following (a1) or (a2)：
(a1) single strand dna shown in the sequence 3 of sequence table；
(a2) sequence 3 is passed through into the substitution of one or several nucleotide and/or lacks and ors add and have with sequence 3
The single strand dna of identical function；
The primer 2 can be following (b1) or (b2)：
(b1) single stranded DNA shown in the sequence 4 of sequence table；
(b2) sequence 4 is passed through into the substitution of one or several nucleotide and/or lacks and ors add and have with sequence 4
The single strand dna of identical function.
The application of above-mentioned DNA fragmentation or above-mentioned special primer pair is also the scope of protection of the invention, for following (e1) or
(e2) or (e3) or (e4)：
(e1) high-yield rice is screened；
(e2) rice of the screening with different output character；
(e3) identify or assist the yield traits of identification rice；
(e4) identify or assist rice of the identification with different output character.
In order to solve the above technical problems, the present invention also provides a kind of kit, including the special primer pair.The examination
It may also include the conventional reagent for extracting oryza sativa genomic dna and/or the conventional reagent for carrying out PCR amplification in agent box
And/or the conventional reagent for being sequenced.
The purposes of the kit is following (e1) or (e2) or (e3) or (e4)：
(e1) high-yield rice is screened；
(e2) rice of the screening with different output character；
(e3) identify or assist the yield traits of identification rice；
(e4) identify or assist rice of the identification with different output character.
The preparation method of the kit also belongs to protection scope of the present invention.The preparation method of the kit includes will
The step of each primer is individually packed in the kit.
The DNA fragmentation, the special primer are to, the kit or any description above method in rice breeding
Using also belonging to protection scope of the present invention.The breeding objective of the rice breeding is to obtain the high rice of yield.
Any of the above-described yield can be single plant yield.Any of the above-described yield can be grain yield.
The present invention also provides a kind of methods of identification advantage allele, include the following steps：By comparing different groups
Biological character difference, determine with advantage character allele；In the different groups, each group is by the equipotential
The individual composition of gene pure.
The biology can be generative propagation biology, and concretely plant, more specifically can be rice.
The character can be that can measure character, and concretely yield traits, more specifically can be Grain yield traits.
It is demonstrated experimentally that the rice with different single plant yield characters, operation letter can be screened using method provided by the invention
Single, accuracy rate is high, has important application value in rice breeding.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
The present invention is further described in detail With reference to embodiment, the embodiment provided is only for explaining
The bright present invention, the range being not intended to be limiting of the invention.
Embodiment 1, the design of primer and synthesis
Chorismic acid is the important intermediate material in rice body.In rice genome, there are several genes to be annotated
For encoding chorismate mutase (EC5.4.99.5).As encoding chorismate mutase 3 (chorismate mutase 3,
CT829390.1；OSCM3 gene (hereinafter referred to as OSCM3 genes)).On the number one chromosome of rice.By big
Preliminary experiment and sequence alignment are measured, the inventors found that there are a kind of two kinds of allele segment (equipotentials in OSCM3 genes
Genetic fragment is named as allele segment OSCM3_a as shown in the sequence 1 of sequence table；Another allele segment such as sequence
Shown in the sequence 2 of list, it is named as allele segment OSCM3_b), they and rice single plant yield have correlation.
Special primer pair is designed according to above two allele segment, is made of primer 1 and primer 2.
Primer 1 (sequence 3 in sequence table)：5’-CTGTTGCCACCTCTCCAGT-3’；
Primer 2 (sequence 4 in sequence table)：5’-TCTCCTCAGCAAGTAGGCTACT-3’.
The foundation of classifying method based on the allele segment in embodiment 2, rice
The method of foundation is as follows：
1st, it with the genomic DNA (about 10~100ng) of rice to be measured for template, is formed using primer 1 and primer 2 special
Primer pair carries out PCR amplification, obtains pcr amplification product.
The response procedures of PCR amplification：95 DEG C 5 minutes；95 DEG C 30 seconds, 60 DEG C 1 minute, 72 DEG C 1 minute, 35 cycle；72
DEG C 8 minutes.
2nd, after completing step 1, pcr amplification product is sequenced, is judged as follows according to sequencing result：If PCR
Amplified production only has one kind, and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_a；If
Pcr amplification product only has one kind, and as shown in sequence 2 in sequence table, then the genotype of rice to be measured is homozygous for OSCM3_b；
If pcr amplification product is two kinds, one kind as shown in sequence 1 in sequence table, as shown in sequence 2 in sequence table then treat by another kind
The genotype for surveying rice is OSCM3_a/OSCM3_b heterozygous.
Since rice varieties are cultigen or Local variety, genotype is high for homozygous individual ratio.
Genotype and the association analysis of rice single plant yield based on the allele segment in embodiment 3, rice
First, the classifying method established according to embodiment 2, the genotype for detecting each rice varieties on probation (the results are shown in Table 1, table 2
It is arranged with table the 3, the 4th).
Table 1
2nd, the single plant yield of different rice varieties is counted
Multiple rice varieties were planted respectively in Beijing Xiangshan District in 2017 (is specifically shown in Table 1, rice varieties title is shown in
The 3rd row are seen in 2nd row, the place of production of rice varieties).After rice maturation, rice plant presses single plant sowing, repeats at least three times, to claim
Amount, is averaged, and obtains the single plant yield (the results are shown in Table the 1, the 5th row) of the kind.
The result of table 1 is shown：The genotype of 17 kinds is homozygous for OSCM3_a in 37 rice varieties, this 17 product
The average single plant yield of kind is 26.23 ± 2.17 grams；The genotype of 20 kinds is homozygous for OSCM3_b in 37 rice varieties
Type, the average single plant yield of this 20 kinds is 31.75 ± 2.18 grams；The average significance test P=0.03 of two kinds of pure and mild types；
Pairs of t- test significance P=0.019 (N=12).
3rd, the method for establishing rice of the screening with different single plant yield characters
The method of rice of the screening with different single plant yield characters be：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located at paddy gene
It is OSCM3 in group, it is described there are two kinds of allelic forms of OSCM3_a (AP003239.3) and OSCM3_b (CP018157.1)
OSCM3_a is as shown in the sequence 1 of sequence table, and the OSCM3_b is as shown in the sequence 2 of sequence table；
(2) judged as follows：Under neighbouring growth conditions, the genotype Rice Population homozygous for OSCM3_b is put down
The average single plant yield of the equal single plant yield Rice Population homozygous for OSCM3_a higher than genotype.
Sequence table
<110>Institute of Botany, Chinese Academy of Sciences
<120>Chorismate mutase nucleotide sequence relevant with rice high yield and its application
<160> 4
<170> PatentIn version 3.5
<210> 1
<211> 1393
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 1
ctgttgccac ctctccagta tccaaaggta atgcataaac tcgtgttctt agacatctag 60
ttcttctctg taggctttca gcattccttt tgttagtata ggcaaacata gtccttcatt 120
gactaatcac taatatatca ggttttatta tttgggttta tgcttgcctc tctctcccta 180
tctctctctc tgaactgaca gtagatgaat tggttatgca tcttatgtat tcgtagatgt 240
tgaattcttc tgtgtgggct tgcgtagctt cttttgtcag tgaaggcaag catgtacctt 300
tgttgactga ccactagtat atcggttctg tggtgggata tcgtgcttac tgtccacctg 360
caacataata caacttttga tacgaagtgg agtaccatct attgagactc acattgctaa 420
ttgattactg tgctgttgta ttgttataag taaatttgga taaaattcat atttttgttc 480
ttgtcttttc taggttttgc atcctattgc tgattctatt aatatcaaca aggagatttg 540
gaaaatgtat tttgatgagc ttcttccaag attagtgaaa gaaggaagtg atggtaatta 600
tggatccagt gctctttgtg acacgatctg cttgcaggta ctacagttag tataccaaat 660
gttctaataa tctggcctcg tactgcttct ggctactgtg ctgtagcttt catttgctca 720
aaataattta gcattagcaa aataattctt gtaaaaccat ttcttgttgt agtattgctt 780
caagatgttc tgctgccaga attttcacca gatgtcattt tcatagtatt agtactatac 840
tgttgttcac tggtgactcg tgcaattagg catttttata ttgggaaatt atgctcacct 900
gttcttgttt tcctgttcta tcatccaggc gctctccaaa agaattcact atggtaagtt 960
tgtggcagag gctaagtttc aagagtctcc tgaagcttac atgcctgcga taatagcaca 1020
ggtttgtgtt ctgttttgct gtataatctg tctttatcat gggaagcaaa taaattccat 1080
gtgttttctt gtacattact ttaggtacat atcaaagttg agtctattat gcaagcggct 1140
aattattttt atctgggaat ttcaggactg cgatcaacta atgcacctcc tcacctatga 1200
aacggtggag cgtgctattg aacatagggt ggaagctaag gctaagatct ttggacagga 1260
ggtggattta ggcgctgaag acaacggcgc tccaccaatg tacaagataa ggcccagttt 1320
ggtggctgaa ctgtacagct acaggatcat gccgctaacc aaggaggttc aagtagccta 1380
cttgctgagg aga 1393
<210> 2
<211> 1396
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 2
ctgttgccac ctctccagta tccaaaggta atgcataaac tcgtgttctt agacatctag 60
ttcttctctg taggctttca gcattccttt tgttagtata ggcaaacata gtccttcatt 120
gactaatcac taatatatca ggttttatta ttatttgggt ttatgcttgc ctctctctcc 180
ctatctctct ctctgaactg acagtagatg aattggttat gcatcttatg tattcgtaga 240
tgttgaattc ttctgtgtgg gcttgcgtag cttcttttgt cagtgaaggc aagcatgtac 300
ctttgttgac tgaccactag tatatcggtt ctgtggtggg atatcgtgct tactgtccac 360
ctgcaacata atacaacttt tgatacgaag tggagtacca tctattgaga ctcacattgc 420
taattgatta ctgtgctgtt gtattgttat aagtaaattt ggataaaatt catatttttg 480
ttcttgtctt ttctaggttt tgcatcctat tgctgattct attaatatca acaaggagat 540
ttggaaaatg tattttgatg agcttcttcc aagattagtg aaagaaggaa gtgatggtaa 600
ttatggatcc agtgctcttt gtgacacaat ctgcttgcag gtactacagt tagtatacca 660
aatgttctaa taatctggcc tcgtactgct tctggctact gtgctgtagc tttcatttgc 720
tcaaaataat ttagcattag caaaataatt cttgtaaaac catttcttgt tgtagtattg 780
cttcaagatg ttctgctgcc agaattttca ccagatgtca ttttcatagt attagtacta 840
tactgttgtt cactggtgac tcatgcaatt aggcattttt atattgggaa attatgctca 900
cctgttcttg ttttcctgtt ctatcatcca ggcgctctcc aaaagaattc actatggtaa 960
gtttgtggca gaggctaagt ttcaagagtc tcctgaagct tacatgcctg cgataatagc 1020
acaggtttgt gttctgtttt gctgtataat ctgtctttat catgggaagc aaataaattc 1080
catgtgtttt cttgtacatt actttaggta catatcaaag ttgagtctat tatgcaagcg 1140
gctaattatt tttatctggg aatttcagga ctgcgatcaa ctaatgcacc tcctcaccta 1200
tgaaacggtg gagcgtgcta ttgaacatag ggtggaagct aaggctaaga tctttggaca 1260
ggaggtggat ttaggcgctg aagacaacgg cgctccacca atgtacaaga taaggcccag 1320
tttggtcgct gaactgtaca gctacaggat catgccgcta accaaggagg ttcaagtagc 1380
ctacttgctg aggaga 1396
<210> 3
<211> 19
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 3
ctgttgccac ctctccagt 19
<210> 4
<211> 22
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 4
tctcctcagc aagtaggcta ct 22
Claims (10)
1. a kind of method for screening high-yield rice is method first or method second；
The method first includes the following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located in rice genome,
For OsCM3, there are two kinds of allelic forms of OsCM3_a and OsCM3_b, the OsCM3_a is described as shown in the sequence 1 of sequence table
OsCM3_b is as shown in the sequence 2 of sequence table；
(2) judged as follows：Under neighbouring growth conditions, the average production of the genotype Rice Population homozygous for OsCM3_b
The average product of the amount Rice Population homozygous for OsCM3_a higher than genotype；
The method second includes the following steps：
(1) using the genomic DNA of rice to be measured as template, using special primer described in claim 4 to carrying out PCR amplification；Such as
Fruit pcr amplification product only has one kind and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OsCM3_a；
If pcr amplification product only has one kind and as shown in sequence 2 in sequence table, the genotype of rice to be measured is homozygous for OsCM3_b
Type；
(2) judged as follows：Under neighbouring growth conditions, the average production of the genotype Rice Population homozygous for OsCM3_b
The average product of the amount Rice Population homozygous for OsCM3_a higher than genotype.
2. a kind of screening technique of high-yield rice is method A or method B；
The method A includes the following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located in rice genome,
For OsCM3, there are two kinds of allelic forms of OsCM3_a and OsCM3_b, the OsCM3_a is described as shown in the sequence 1 of sequence table
OsCM3_b is as shown in the sequence 2 of sequence table；
(2) judged as follows：Under equal growth conditions, the average product of the genotype Rice Population homozygous for OsCM3_b
It is above the average product of the genotype Rice Population homozygous for OsCM3_a；
The method B includes the following steps：
(1) using the genomic DNA of rice to be measured as template, using special primer described in claim 4 to carrying out PCR amplification；Such as
Fruit pcr amplification product only has one kind and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OsCM3_a；
If pcr amplification product only has one kind and as shown in sequence 2 in sequence table, the genotype of rice to be measured is homozygous for OsCM3_b
Type；
(2) judged as follows：Under equal growth conditions, the average product of the genotype Rice Population homozygous for OsCM3_b
It is above the average product of the genotype Rice Population homozygous for OsCM3_a.
3. a kind of seed selection method for rice is method third or method fourth；
The method third includes the following steps：
(1) genotype of the rice based on gene-specific fragments to be measured is detected；The gene-specific fragments are located in rice genome,
For OsCM3, there are two kinds of allelic forms of OsCM3_a and OsCM3_b, the OsCM3_a is described as shown in the sequence 1 of sequence table
OsCM3_b is as shown in the sequence 2 of sequence table；
(2) genotype is the purpose rice that the homozygous rice of OsCM3_b is selection and breeding；
The method fourth includes the following steps：
(1) using the genomic DNA of rice to be measured as template, using special primer described in claim 2 to carrying out PCR amplification；Such as
Fruit pcr amplification product only has one kind and as shown in sequence 1 in sequence table, then the genotype of rice to be measured is homozygous for OsCM3_a；
If pcr amplification product only has one kind and as shown in sequence 2 in sequence table, the genotype of rice to be measured is homozygous for OsCM3_b
Type；
(2) genotype is the purpose rice that the homozygous rice of OsCM3_b is selection and breeding.
4.DNA segments are OsCM3_b；
The OsCM3_b is following (d1) or (d2)：
(d1) DNA molecular shown in the sequence 2 of sequence table；
(d2) sequence 2 by the substitution of one or several nucleotide and/or is lacked and ored add and with sequence 2 with identical
The DNA molecular of function.
5. special primer pair is made of primer 1 and primer 2；
The primer 1 is following (a1) or (a2)：
(a1) single strand dna shown in the sequence 3 of sequence table；
(a2) sequence 3 by the substitution of one or several nucleotide and/or is lacked and ored add and with sequence 3 with identical
The single strand dna of function；
The primer 2 is following (b1) or (b2)：
(b1) single stranded DNA shown in the sequence 4 of sequence table；
(b2) sequence 4 by the substitution of one or several nucleotide and/or is lacked and ored add and with sequence 4 with identical
The single strand dna of function.
6. a kind of kit, including special primer pair described in claim 5；
The purposes of the kit is following (e1) or (e2) or (e3) or (e4)：
(e1) high-yield rice is screened；
(e2) rice of the screening with different output character；
(e3) identify or assist the yield traits of identification rice；
(e4) identify or assist rice of the identification with different output character.
7. the preparation method of kit described in claim 6, including each primer in kit described in claim 6 is single respectively
Solely the step of packaging.
8. the application of special primer pair described in DNA fragmentation described in claim 4 or claim 5, for following (e1) or (e2) or
(e3) or (e4)：
(e1) high-yield rice is screened；
(e2) rice of the screening with different output character；
(e3) identify or assist the yield traits of identification rice；
(e4) identify or assist rice of the identification with different output character.
It is 9. special described in DNA fragmentation described in any the method or, claim 4 or, claim 5 in claim 1-3
Kit described in primer pair or, claim 6, the application in rice breeding.
10. a kind of method of identification advantage allele, includes the following steps：It is poor by comparing the biological character of different groups
It is different, determine the allele with advantage character；In the different groups, each group is by homozygous of the allele
Body forms.
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| CN110499382A (en) * | 2019-08-30 | 2019-11-26 | 中国科学院植物研究所 | A kind of pyruvate kinase allele segment and its application increasing rice yield |
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| CN1995357A (en) * | 2006-01-05 | 2007-07-11 | 华中农业大学 | Gene for controlling rice ear sprouting period and its uses |
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| CN1995357A (en) * | 2006-01-05 | 2007-07-11 | 华中农业大学 | Gene for controlling rice ear sprouting period and its uses |
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| CN110499382A (en) * | 2019-08-30 | 2019-11-26 | 中国科学院植物研究所 | A kind of pyruvate kinase allele segment and its application increasing rice yield |
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