CN108179168A - A kind of industrialized producing technology of deer heart protein oligopeptide - Google Patents
A kind of industrialized producing technology of deer heart protein oligopeptide Download PDFInfo
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- CN108179168A CN108179168A CN201810262136.8A CN201810262136A CN108179168A CN 108179168 A CN108179168 A CN 108179168A CN 201810262136 A CN201810262136 A CN 201810262136A CN 108179168 A CN108179168 A CN 108179168A
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- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
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Abstract
The present invention provides a kind of industrialized producing technologies of deer heart protein oligopeptide.Specific steps include：1st, raw material, 2, slice, 3, impregnate thaw, 4, rub, 5, degreasing, 6, defibrination, 7, saltout, 8, press filtration, 9, enzymolysis, 10, ultrafiltration oligopeptide, 11, nanofiltration retention concentration, desalination, removing impurities matter, 12, degerming, 13, it is filling, 14, freeze-drying, 15, pressure bottle cap and plug, label.The method of the present invention is simple for process, reasonable design, makes full use of the natural resources, it can be achieved that the industrialization and large-scale production of high value added utilization.The present invention uses food-grade albumen enzyme hydrolysis, safe, without side-effects；The present invention can directly take as health products, function food additive or nutraceutical, while be also used as cosmetic additive agent safe handling.
Description
Technical field
The present invention relates to a kind of industrialized producing technologies of deer heart protein oligopeptide, belong to medical industry, Chinese patent drug, function
Health products and cosmetic additive agent field.
Background technology
China is foster deer and using the earliest country of deer product in the world, has the foster deer history and 1300 of more than 260 years
Deer product medicinal history for many years.The artificial culture technology of deer is ripe, and has been detached from the dependence to wildlife resource,
The deer heart for propagating deer artificially can be as a kind of novel material of extraction collagen polypeptide.It is main more to lack deer heart product in the market
The intensive processing product such as health products, foods and cosmetics of raw material.Domestic existing deer product is mainly primary raw material or directly
Using simple processing, most of product uses traditional deer product processing technique, and the bioavilability for leading to product is not high.Product
Level is low, and intensive processing product is few, and technology content is relatively low, and added value of product is not high.Main problem is that deer product processing enterprise lacks
Lack the application of modern technology to promote the deer updating and upgrading of a product.With being growing for the albumen oligopeptide market demand, open
The deer heart protein oligopeptide production technology of highly effective and safe environmental protection of the hair with biological health activity, to scientific research and practical application
It is respectively provided with significance.
Invention content
The present invention is to provide the industrialization of the deer heart protein oligopeptide that output capacity is high, purity is high a kind of under above-mentioned background
Production technology.
The present invention includes the following steps：1st, raw material, 2, slice, 3, immersion defrosting, 4, rubbing, 5, degreasing, 6, defibrination, 7, salt
Analysis, 8, press filtration, 9, enzymolysis, 10, ultrafiltration oligopeptide, 11, nanofiltration retention concentration, desalination, removing impurities matter, 12, degerming, 13, it is filling,
14th, freeze-drying, 15, pressure bottle cap and plug, labelling.It is digested using zymotechnic, collects the peptide fragment of suitable molecular weight, used
Ultrafiltration membrane technique prepares the deer heart protein oligopeptide for having a variety of physiological active functions by concentration, vacuum freezedrying.
A kind of industrialized producing technology of deer heart protein oligopeptide, its step are as follows：
1st, raw material
Raw material selects complete fresh jelly, cleaning, the deer heart frozen manually to deoil.
2nd, it is sliced
The deer heart block burnishing surface frozen is lowered into the material path of slicer, it is made to be cut into the thin slice of 0.3-0.6cm thickness.
3rd, it impregnates and thaws
The deer heart of slice is put into stainless steel rinse bath, injects pure water, impregnates 48h-72 h, until the deer heart becomes soft shape
State.
4th, it rubs
It after pulling the deer heart for being sliced soft extraction of thawing out water drenching, puts in meat grinder, is rubbed into granularity as 0.1-0.2cm
Fragment.
5th, degreasing
By the degreaser soak degreasing 1-2d of the deer heart of rubbing 1.0-2.0% acetums, finally with 30-50 DEG C of warm water rinse 2
It is secondary, obtain the deer heart that degreasing is impregnated.
6th, defibrination
The deer heart after degreasing is worn into slurries with bone mud barreling, particle is become and refines slurries in 20~50 μm of sizes.
7th, it saltouts
Fine grinding slurries obtained are adjusted into pH value 4.0~4.5, NaCl powder is added in and saltouts, it is stirring while adding, make collagen egg
After white precipitation completely, standing 12~for 24 hours, obtain collagen mixed liquor.
8th, press filtration
Mixed liquor is uniformly routed on sheet frame filter cloth, conveying mixed liquor starts 1~6h of press filtration, passes through the aperture regulation of outlet valve
Press filtration progress is controlled, filter cake is discarded, obtains transparent filtrate.
9th, it digests
It is 9.0~9.5 that slurries after press filtration are adjusted pH value, under the conditions of 50 DEG C~60 DEG C, adds in 1-3% trypsase, makes
Deer heart protein enzyme digestion reaction 8h~10h, obtains after being stirred continuously in enzymolysis process with 30 turns/min containing oligopeptide mixed liquor.
10th, ultrafiltration oligopeptide
Enzymolysis liquid is subjected to classification ultrafiltration through reverse osmosis ultrafiltration unit, the ultrafilter of molecular weight 75KDa is first passed through, using molecule
Measure the ultrafilter filtering of 3KDa.The peptide of suitable molecular weight segment enters collector through film, and the peptide of large fragment continues and enzyme effect
It digests again, then ultrafiltration obtains oligopeptide filtered fluid.Its temperature is warming up to 80~95 DEG C of progress high temperature enzyme deactivations after ultrafiltration.
11st, nanofiltration retention concentration, desalination, removing impurities matter
It is to be concentrated in the nanofiltration device of 500Da by the molecular weight that albumen oligopeptide filtrate is pumped into reverse osmosis nanofiltration unit.Again will
Concentrate adds same volume water elution 3-5 times, obtains and is concentrated into the nanofiltration of the minimum volume that water content only has 3-5% or so and cuts
Stay liquid.It will collect containing the nanofiltrations filtered fluid such as other substances in raw material and inorganic salts, carry out purified treatment, the nanofiltration of collection is cut
It is oligopeptide concentrate to stay liquid.
12nd, degerming
After concentrate is filtered using molecular weight 60-80KDa hollow fiber membrane ultrafiltration devices, enter back into 0.22 μm of micropore filter element and carried out
Filter, carries out degerming by way of retention.
13rd, it is filling
Oligopeptide concentrate after degerming is carried out filling with automatic filling machine, is fitted into and is cleaned in sterile 10ml cillin bottles, dosage
Control ensures the stabilization of net content within 5ml, per bottled amount 200mg, and pressurizes rubber stopper plug to state of partly jumping a queue, obtains
To canned product.
14th, it is freeze-dried
（1）Pre-freeze cures：The cillin bottle of canned concentrate is placed on the partition board in vacuum freeze drier body feed tank, pre-freeze
Curing ensures that no liquid exists.
（2）Lyophilization：Freeze dryer starts vacuum system, takes slow heating, carries out lyophilization.
（3）Parsing-desiccation：After lyophilization, with 3 DEG C per hour of heating rate, 40~50 DEG C are slowly warming up to, is completed
Parsing-desiccation is lyophilized.
15th, bottle cap and plug, labelling are pressed
Aseptically machinery pressure bottle cap and plug after freeze-drying, makes it post label into labelling machine.Purity of protein height is made
Oligopeptide component content up to 99.5%, relative molecular mass less than 1000Da is averagely more than 92% deer heart oligopeptide finished powder.
Compared with prior art, main advantages of the present invention are：
1st, as a further improvement on the present invention, the present invention makes full use of the processing waste deer heart for raw material, is easy to get, cost
It is low, solve the problems, such as that utilization rate existing for existing resource and added value are low.Deer the heart channel of Hang-Shaoyin strength is sliced by present invention process process,
The anxious flakiness of deer will directly be frozen under without thawed state, better assure that the quality of raw material.Make after the mill grinding of bone mud
Raw material is finer and smoother, and uniformly crushing effect is good, and advantage is created for the follow-up oligomeric peptide content for improving extraction.
2nd, as a further improvement on the present invention, the present invention is saltoutd in the extraction of albumen oligopeptide using NaCl powder
Method first collagen is cemented out, and after saltouing carry out plate and frame filter press press filtration, then press filtration is obtained slurries progress
Enzymolysis.Single enzyme and compound protease screening verification have been carried out to the enzyme used in deer heart collagen before enzymolysis, selected by verifying
With the trypsase with complex enzyme function, and after taking appropriate enzymolysis, ultrafiltration, the enzymolysis and extraction side repeatedly that digests again
Method destroys partial peptide key and forms the albumen oligopeptide that molecular weight is less than 1000Da, to not reaching the extracting solution of small-molecular peptides
Further enzymolysis is realized, finally takes high temperature enzyme deactivation, enzymolysis process design resolves into deer heart collagen to be less than
The component content of the albumen oligopeptide of 1000Da molecular weight is averagely up to 92%.Thus improve deer heart protein activity and utilization rate.
3rd, as a further improvement on the present invention, it is more than filtering method present invention employs reverse osmosis ultrafiltration unit, passes through
Macromolecular substances are retained repeatedly, and filtering, separation are achieved the purpose that through small-molecule substance.First pass through the super of molecular weight 75KDa
Filter filters, and is filtered using the ultrafilter of molecular weight 3KDa, and molecular cut off is in 2000-100000Da protein peptides, general
The small-molecular-weight segment oligopeptide of below 1000Da enters collector through film, and the peptide of large fragment continues and enzyme effect, then row is super
Filter.It ensure that the concentration output of oligomeric peptide product and making full use of for raw material.
4th, as a further improvement on the present invention, the present invention uses nanofiltration device of the molecular weight for the reverse osmosis collecting and filtering apparatus of 500Da,
Oligopeptide is obtained by nanofiltration and retains concentrate, while causes salt, impurity as nanofiltration filtered fluid is deviate from.It substantially increases
The purity of albumen oligopeptide so that albumen oligopeptide purity is up to 99.5%.Simultaneously by the nanofiltration containing salt, impurity of collection
Filtrate is pumped into biochemical treatment tank, carries out purified treatment, is discharged after up to standard so that production technology has reached environmentally protective standard.
5th, as a further improvement on the present invention, the method that the present invention uses physics degerming, according to point of albumen oligopeptide
Son amount and bacteria molecule amount have selected molecular weight 60-80KDa hollow fiber membrane ultrafiltration devices to refilter, after filtering, into 0.22 micron
The small filter of degerming carries out degerming.The method of the degerming, which has, does not destroy active ingredient, does not increase other pollutions, efficient, degerming
Effect is good, effectively increases the shelf-life of the oligomeric peptide product of albumen.
6th, as a further improvement on the present invention, the production of albumen oligopeptide of the present invention is in high-efficiency and continuous automated production
It is produced under system, entire technological process is mostly to be produced under sealing condition, such as filling using automatic filling machine progress, especially this hair
Bright freeze-drying takes slow heating technology, and temperature in entire sublimation process is made to be no more than eutectic point, ice crystal will not be caused to melt
Change and influence the quality of oligomeric Gly-His-Lys, ensure that the high activity of the oligomeric peptide product of albumen.Environment-friendly advantage of the present invention protrudes, and does not use
Toxic reagent, non-pollutant discharge reach clean manufacturing, environmentally protective, product safety health, with preferable industrialized production
Advanced and practicability.It is that the deer heart is reasonable using new approach is opened, is deer heart protein oligopeptide industrialization and scale metaplasia
Production provides effective process.
Specific embodiment
Embodiment 1
A kind of industrialized producing technology of deer heart protein oligopeptide, its step are as follows：
1st, raw material
Raw material selects complete fresh jelly, cleaning, and the deer heart frozen manually to deoil ensures deer heart surface noresidue clot or dirt
Object.
2nd, it is sliced
The deer heart block burnishing surface frozen is lowered into the material path of slicer, makes its temperature at -12 DEG C to -6 DEG C, is pressed automatic
Button is fed, pusher starts pusher forward, starts to cut, and adjusts cylinder trip 70cm, air pressure 0.4-0.5Mpa, knife rotation speed
It spends for 435 turns/min, it is made to be cut into the thin slice of 0.3-0.6cm thickness.
3rd, it impregnates and thaws
The deer heart of slice is put into stainless steel rinse bath, injects pure water, height of water level will be more than deer heart 10-15cm, stir
Uniformly, 48h-72 h are impregnated, until the deer heart becomes doughy state, while impregnate removing blood constituent.
4th, it rubs
After pulling the deer heart for being sliced soft extraction of thawing out water drenching, put in meat grinder, rubbed, being rubbed into granularity is
The fragment of 0.1-0.2cm.
5th, degreasing
By the deer heart of rubbing 1.0-2.0% acetum degreaser soak degreasing 1-2d, temperature is filled at 25-30 DEG C per 2h-3h
Point stirring 1 time, filters off supernatant liquid, and it is primary to repeat degreasing as stated above, finally with 30-50 DEG C of warm water rinse 2 times, obtains
The deer heart that degreasing is impregnated.
6th, defibrination
The deer heart after degreasing is iterated through bone mud to grind 3-4 times, particle is become in 20~50 μm of size slurries, adds water to 500-
1000L water level lines impregnate 1~2d, remove soaking water, and the soaked deer heart is ground defibrination 1 time again in bone mud, becomes particle
Slurries are refined in 20~50 μm of sizes.
7th, it saltouts
Fine grinding slurries input 3000L obtained is extracted into big tank, 100kg slurries add water 3000L, with 0.5mol/LHCl solution tune
PH value 4.0~4.5 is saved, is stirred evenly, NaCl powder is then added in and saltouts, it is stirring while adding, keep 30r/min stirring slurries
Liquid, hour or so add 100kg sodium chloride.After collagen is precipitated completely, standing 12~for 24 hours, obtain collagen
Mixed serum.
8th, press filtration
The sheet frame of plate and frame filter press is laid into No. 3927 filter clothes, the addition of 0.5% super-cell is filled to the tank of enzymolysis liquid
In, it then stirs, is configured to the liquid material to be filtered of the enzymolysis liquid mixed liquor added with filter aid, it is later that mixed liquor is uniform
Be routed on sheet frame filter cloth, slowly open inlet valve, the outlet valve of filter pressing pump, start press filtration relay pump, pressure for 0.1~
1.0MPa, conveying mixed liquor start press filtration, control press filtration progress by the aperture regulation of outlet valve, filter is discarded after the completion of press filtration
Cake obtains transparent filtering slurries.
9th, it digests
Slurries after press filtration are added in 2500L enzymatic vessels, add 7L water according to every 3L slurries, 600L slurries about add water 1400L,
The pH value for adding sodium hydroxide adjusting slurries is 7.0~9.0, and be heated under the conditions of 50 DEG C~60 DEG C, then by 1-3%
Trypsase is added in the enzymatic vessel, makes deer heart protein and tryptose enzyme digestion reaction 8h~10h, with 30 in enzymolysis process
Turn/min is stirred continuously, the mixed liquor containing a large amount of oligopeptides is obtained by enzymolysis.
9th, ultrafiltration oligopeptide
Oligopeptide mixed liquor after enzymolysis is subjected to classification ultrafiltration through reverse osmosis ultrafiltration unit, first passes through the super of molecular weight 75KDa
Filter filters, and is filtered using the ultrafilter of molecular weight 3KDa.Ensure pressure 3.0-5.0MPa, flow velocity 1000- in ultra-filtration process
1200L/h adds water 800L ultrafiltration 2-3 times every time, and the peptide of suitable molecular weight segment enters collector through film after ultrafiltration, retention
The peptide of large fragment continues and enzyme effect digests again, then ultrafiltration obtains the peptide of suitable molecular weight segment.It is low to be so repeatedly available albumen
Poly- peptide filtered fluid.Filtered fluid temperature is warming up to 80~95 DEG C of progress high temperature enzyme deactivations, 10~25min after ultrafiltration.
10th, nanofiltration retention concentration, desalination, removing impurities matter
By albumen oligopeptide filtrate be pumped into reverse osmosis nanofiltration unit molecular weight be 500Da ultrafilter in, keep pressure 2.5~
3.5MPa under conditions of temperature is 15~45 DEG C, carries out reverse osmosis nanofiltration concentration, until nanofiltration retentate fluid is concentrated into water content only
There is a minimum volume of 3-5% or so, then concentrate is added into same volume water elution 3-5 times, similary obtain is concentrated into water content only
There is the minimum volume nanofiltration retentate fluid of 3-5% or so.It is collected nanofiltration filtered fluid and nanofiltration retentate fluid respectively, collection is contained
The nanofiltrations filtered fluids such as other impurities substance and inorganic salts in raw material are pumped into biochemical treatment tank and carry out purified treatment, heel row up to standard
It puts, the nanofiltration retentate fluid of collection is deer heart oligopeptide concentrate, enters degerming process to it.
11st, degerming
After being filtered using molecular weight 60-80KDa hollow fiber membrane ultrafiltration devices, enter back into 0.22 μm of micropore filter element and be filtered, pressing
Under the conditions of power 2.0-3.0 MPa, ultrafiltration feed velocity 1.2-1.8L/min, to deer heart oligopeptide concentrate by way of retention
Degerming is carried out, after degerming, is sealed with stainless steel sealing bucket into filling.
12nd, it is filling
Oligopeptide concentrate after degerming is carried out with automatic filling machine it is filling, using cleaning in sterile 10ml cillin bottles.West
Woods bottle first pass through ultraviolet light pass-through box enter ultrasonic bottle washing machine between wash bottle it is per minute wash 200 bottles of washed bottles and enter dryer exist
It is dried under 260 DEG C of 280 DEG C of preheating temperature, 300 DEG C of sterilising temp, heat preservation programs, completes 10,000 bottles of cillin bottle sterilizings per hour and dry
It is dry.Cillin bottle is filling into sterile beginning, and dosage is controlled within 5ml, ensures the stabilization of net content, per bottled amount 200mg,
Filling 200 bottles per minute, and after pressurized rubber plug to state of partly jumping a queue, canned product is entered in vacuum freeze drier
Freeze-drying.
14th, it is freeze-dried
（1）Pre-freeze cures
By canned concentrate cillin bottle, it is placed on the partition board in vacuum freeze drier dry storehouse, pre-freeze solidification temperature -30
~-45 DEG C, and 5~8h is kept, moisture in concentrate is made fully to freeze, it is ensured that no liquid exists.
（2）Lyophilization：Freeze dryer starts vacuum system, and pressure in storehouse is evacuated to 30~50pa, takes slow heating, with
1 DEG C of heating rate per hour is slowly warming up to -20~-25 DEG C, keeps the temperature 6~8h, then with 2 DEG C per hour of heating rate, delay
It is slow to be warming up to -10 DEG C~-12 DEG C, 3~6h is kept the temperature, carries out lyophilization.
（3）Parsing-desiccation：After lyophilization, then with 3 DEG C per hour of heating rate, 40~50 DEG C are slowly warming up to, pressure
Power is 10~20Pa.6~8h is kept the temperature, completes parsing-desiccation freeze-drying.
15th, aseptically machinery pressure bottle cap and plug after bottle cap and plug, labelling freeze-drying is pressed, mark is posted into labelling machine
Label.Purity of protein is made and is up to oligopeptide component content of the 99.5%, relative molecular mass less than 1000Da averagely more than 92%
Deer heart oligopeptide finished powder.
Embodiment 2
It weighs 100kg and cleans the deer heart frozen, be put into after slicer is cut into the thin slice of 0.3-0.6cm thickness, inject pure water, impregnate
16-20h is into doughy state.Put the fragment rubbed in meat grinder into granularity is 0.1-0.2cm.With 1.0-2.0% acetum degreasings
1-2d, with 30-50 DEG C of warm water rinse 2 times.Particle, which is worn into, with bone mud barreling refines slurries in 20~50 μm of sizes.Adjust pH value
4.0~4.5, it adds in NaCl powder and saltouts, with plate and frame filter press press filtration, will obtain transparent filtrate and adjust pH value is 9.0
~9.5, under the conditions of 50 DEG C~60 DEG C, add in 1-3% trypsin digestions reaction 8h~10h.Enzymolysis liquid is classified ultrafiltration, first
By the ultrafilter of molecular weight 75KDa, filtered using the ultrafilter of molecular weight 3KDa.Its temperature is warming up to 80 after ultrafiltration~
95 DEG C of progress high temperature enzyme deactivations.Again through molecular weight to be concentrated in the nanofiltration device of 500Da.Elution 3-5 times, makes to be concentrated into water content
The only minimum volume of 3-5% or so.The nanofiltration filtered fluid of impure and inorganic salts etc. is subjected to purified treatment.Oligopeptide is dense
After contracting liquid is using the filtering of molecular weight 60-80KDa hollow fiber membrane ultrafiltration devices, 0.22 μm of micropore filter element filtration sterilization is entered back into.With certainly
Dynamic bottle placer progress is filling, and 10ml cillin bottles dosage is controlled within 5ml, per bottled amount 200mg.Vacuum freeze drier freezes
It is dry.Purity of protein is made and is up to oligopeptide component content of the 99.5%, relative molecular mass less than 1000Da averagely up to 92%
Deer heart oligopeptide finished powder.100kg, which freezes fresh deer heart raw material, can averagely produce the oligomeric Gly-His-Lys of 3.675kg.
Claims (1)
1. a kind of industrialized producing technology of deer heart protein oligopeptide, its step are as follows：
（1）Raw material
The deer heart that raw material selection freezes；
（2）Slice
It is cut into the thin slice of 0.3-0.6cm thickness；
（3）It impregnates and thaws
48h-72 h are impregnated in water filling；
（4）It rubs
Rub into the fragment that granularity is 0.1-0.2cm；
（5）Degreasing
By the degreaser soak degreasing 1-2d of the deer heart of rubbing 1.0-2.0% acetums, finally with 30-50 DEG C of warm water rinse 2
It is secondary, obtain the deer heart that degreasing is impregnated；
（6）Defibrination
The deer heart after degreasing is worn into slurries with bone mud barreling, becomes particle in 20~50 μm of grinding slurries；
（7）It saltouts
Fine grinding slurries obtained are adjusted into pH value 4.0~4.5, NaCl powder is added in and saltouts, it is stirring while adding, make collagen egg
After white precipitation completely, standing 12~for 24 hours, obtain collagen mixed liquor；
（8）Press filtration
Mixed liquor is uniformly routed on sheet frame filter cloth, conveying mixed liquor starts 1~6h of press filtration, discards filter cake, obtains transparent
Filtrate；
（9）Enzymolysis
It is 9.0~9.5 that slurries after press filtration are adjusted pH value, under the conditions of 50 DEG C~60 DEG C, adds in 1-3% trypsase, makes
Deer heart protein enzyme digestion reaction 8h~10h, obtains after being stirred continuously in enzymolysis process with 30 turns/min containing oligopeptide mixed liquor；
（10）Ultrafiltration oligopeptide
Enzymolysis liquid is subjected to classification ultrafiltration through reverse osmosis ultrafiltration unit, the ultrafilter of molecular weight 75KDa is first passed through, using molecule
Measure the ultrafilter filtering of 3KDa；The peptide of suitable molecular weight segment enters collector through film, and the peptide of large fragment continues and enzyme effect
It digests again, then ultrafiltration obtains oligopeptide filtered fluid；Its temperature is warming up to 80~95 DEG C of progress high temperature enzyme deactivations after ultrafiltration；
（11）Nanofiltration retention concentration, desalination, removing impurities matter
It is to be concentrated in the nanofiltration device of 500Da by the molecular weight that albumen oligopeptide filtrate is pumped into reverse osmosis nanofiltration unit；Again will
Concentrate adds same volume water elution 3-5 times, obtains and is concentrated into the nanofiltration retentate fluid that water content only has the minimum volume of 3-5%；
It will collect containing the nanofiltrations filtered fluid such as other substances in raw material and inorganic salts, carry out purified treatment, the nanofiltration retentate fluid of collection
For oligopeptide concentrate；
（12）Degerming
After concentrate is filtered using molecular weight 60-80KDa hollow fiber membrane ultrafiltration devices, enter back into 0.22 μm of micropore filter element and carried out
Filter, carries out degerming by way of retention；
（13）It is filling
It is filling in cillin bottle；
（14）Freeze-drying
（1）Pre-freeze cures
By canned concentrate cillin bottle, it is placed on the partition board in vacuum freeze drier dry storehouse, pre-freeze solidification temperature -30
~-45 DEG C, and 5~8h is kept, moisture in concentrate is made fully to freeze, it is ensured that no liquid exists；
（2）Lyophilization：Freeze dryer starts vacuum system, and pressure in storehouse is evacuated to 30~50pa, takes slow heating, with every small
When 1 DEG C of heating rate, be slowly warming up to -20~-25 DEG C, keep the temperature 6~8h, then with 2 DEG C per hour of heating rate, slowly rise
Temperature keeps the temperature 3~6h to -10 DEG C~-12 DEG C, carries out lyophilization；
（3）Parsing-desiccation：After lyophilization, then with 3 DEG C per hour of heating rate, 40~50 DEG C are slowly warming up to, pressure is
10~20Pa；6~8h is kept the temperature, completes parsing-desiccation freeze-drying；
（15）Press bottle cap and plug, labelling
Aseptically machinery pressure bottle cap and plug after freeze-drying, makes it post label into labelling machine；Purity of protein height is made
Oligopeptide component content up to 99.5%, relative molecular mass less than 1000Da is averagely more than 92% deer heart oligopeptide finished powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810262136.8A CN108179168A (en) | 2018-03-28 | 2018-03-28 | A kind of industrialized producing technology of deer heart protein oligopeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810262136.8A CN108179168A (en) | 2018-03-28 | 2018-03-28 | A kind of industrialized producing technology of deer heart protein oligopeptide |
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| CN110452948A (en) * | 2019-09-12 | 2019-11-15 | 北华大学 | Deer heart protein hydrolysate and its preparation method and application |
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| CN110452948A (en) * | 2019-09-12 | 2019-11-15 | 北华大学 | Deer heart protein hydrolysate and its preparation method and application |
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