CN108179159A - A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue - Google Patents
A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue Download PDFInfo
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- CN108179159A CN108179159A CN201711317723.4A CN201711317723A CN108179159A CN 108179159 A CN108179159 A CN 108179159A CN 201711317723 A CN201711317723 A CN 201711317723A CN 108179159 A CN108179159 A CN 108179159A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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Abstract
The invention discloses a kind of method of the achievable efficient enzyme hydrolysis production fermentable sugars of corncob residue, step is as follows：(1) raw material prepares；(2) electroplating effluent recycling；(3) collect the corncob residue after reaction to wash repeatedly for several times until being in neutrality, corncob residue after must washing；(4) enzyme hydrolysis obtains hydrolyzate, that is, completes the efficient enzyme hydrolysis production fermentable sugars of corncob residue.This method utilizes electroplating effluent recycling technical finesse corncob residue, increases the hydrophilicity of lignin in fibrous raw material, makes it easier to dissolve out, so as to improve corncob residue enzyme hydrolysis yield；This method can make corncob residue obtain higher value application, the input cost of enzyme hydrolysis is reduced simultaneously, improve enzyme hydrolysis efficiency, and provide raw material basis for subsequent processing and utilization or production high value added product, so as to expand the application field of corncob residue and range, and it can effectively reduce the usage amount of wood fiber raw material.
Description
Technical field
The invention belongs to biomass resource with agricultural waste effectively using the technical field being combined, especially one kind can
The method for realizing the efficient enzyme hydrolysis production fermentable sugars of corncob residue.
Technical background
With economic continuous development, people are growing day by day for the demand of the energy, especially to coal, oil, natural
The degree of dependence of the fossil energies such as gas is increasing, and energy crisis becomes one of significant challenge that human society faces.Meanwhile by
In a large amount of burnings of fossil fuel, caused by a series of environment such as air pollution, acid rain, greenhouse effects and depletion of the ozone layer ask
Inscribe getting worse.Therefore, under energy crisis and the double challenge of environmental pollution, seek a kind of novel, reproducible cleaning
The energy is particularly important to substitute fossil energy.
Biorefinery be using biomass resource as raw material, by certain refining equipments, integrated use it is a variety of biology,
Chemistry or physics method increases the added value of biomass resource or carries out renewable resources utilization to biomass resource, finally
It is made to be converted into various bio-fuels or other chemicals and biomaterial with high added value etc..It has been reported that pass through life
The bio-fuel of substance refining techniques production is referred to as best oil replacement product, thus, biorefinery is known as developing
The best mode of regenerative resource.
Wherein, biomass enzyme hydrolysis is a kind of novel, green biomass resource utilization mode.By to biomass material
Enzyme hydrolysis is carried out, and obtained monosaccharide is applied to zymotechnique, it is raw to produce biological butanol and alcohol fuel etc. by fermentation
Biomass fuel, it can also be used to produce other products such as food, drug or feed.Therefore, the enzyme hydrolysis process of fibrous raw material is being eaten
Product, fermentation, weaving and energy etc. have good application prospect.But for current research, majority is using wood
Matter fibrous raw material directly carries out enzyme hydrolysis extraction glucose and is processed further being applied to other fields again.This method is carried
The glucose transformation efficiency taken is not high and enzyme hydrolysis early investment cost is excessively high, is unfavorable for industrializing, and majority research only rests on
The laboratory research stage.Therefore, the side of enzyme hydrolysis input cost can be reduced again by finding a kind of inversion rate of glucose that can either improve
Method has important role to the development of human society.
Chemical Pretreatment is one of method of wood fiber biomass pretreatment.Mainly include low-kappa number, NaOH locates in advance
Reason is pre-processed with sulfonation.Wherein, low-kappa number pre-processes relatively conventional with NaOH, although low-kappa number can with NaOH pretreatments
Realize that still cellulose causes raw fibre to efficient enzyme hydrolysis efficiency from can also occur to degrade under acid or alkaline environment
In some glucose be lost in.And sulfonation pretreatment be then based on sulfite pulping technology grow up one kind it is novel
Wooden Biomass pretreatment of raw material technology, it is applicable not only to wood fiber raw material, former to the higher non-wood-fiber containing lignin
Material also has good effect.Sulfonation pretreatment mainly makes the lignin in lignocellulose raw material that sulfonating reaction occur so as to increase wood
The hydrophily of element makes it be easier to dissolve out, smaller to the effect of cellulose and hemicellulose, for improving the enzyme hydrolysis efficiency of raw material
It is highly beneficial.Compared with other pretreatment modes, relatively low with energy consumption, the corrosivity of equipment is smaller, and reaction condition is more mild
The advantages that.Therefore, it is gradually paid close attention in recent years by related researcher.
Corncob is that corn ear sloughs the cob after seed, is a kind of abundant agricultural wastes of standing crop, and complete
Have in world wide and be widely distributed.It is shown according to the data of United States Department of Agriculture, 2013~2014 years, the corn yield in the U.S.
It is 3.51 × 108T, wherein corncob are calculated by 25%~30%, then can about generate 5.62~6.32 × 107The corncob of t is residual
Slag.In addition, also it has been reported that Chinese corn annual output in 2013 is 2.15 × 108T, it means that about 3.3 × 107
~3.7 × 107The corncob of t can be utilized.Due to containing abundant hemicellulose (xylan) and lignin in corncob
The advantages that content is low, and bulk density is high, and energy is high, and grain size is small, therefore, the production furfural that is industrially widely used in recent years,
The products such as xylose, xylo-oligosaccharide and xylitol.A large amount of corncob residue can be generated in these chemical process, these
Residue directly burns usually as fuel or directly discards in field, this is not only the waste to resource, while also results in one
Serial problem of environmental pollution.The main component of corncob residue is the substance extracted after hemicellulose, and it is dry that content of cellulose accounts for it
More than half of weight；Simultaneously as it passes through industrial utilization, structure becomes loose porous, has preferably enzymolysis property.Cause
This, carries out cellulose hydrolyzation as raw material using corncob residue and prepares fermentable sugars, and then produce applied to subsequent fermentation
Alcohol fuel etc. has good economic value and application prospect.
At present, the method about the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue is not yet found.
By retrieval, not yet find and the relevant patent publication us of present patent application.
Invention content
It is an object of the invention in place of overcome the deficiencies in the prior art, provide a kind of achievable efficient enzyme of corncob residue
The method of hydrolysis production fermentable sugars, this method utilize electroplating effluent recycling technical finesse corncob residue, increase in fibrous raw material
The hydrophilicity of lignin makes it easier to dissolve out, so as to improve corncob residue enzyme hydrolysis yield；This method can make corncob residual
Slag obtains higher value application, while reduces the input cost of enzyme hydrolysis, improves enzyme hydrolysis efficiency, and for subsequent processing and utilization or
It produces high value added product and raw material basis is provided, so as to expand the application field of corncob residue and range, and can effectively drop
The usage amount of low wood fiber raw material.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue, step are as follows：
(1) raw material prepares：By corncob residue through air-drying, crushing, screening, corncob residue after must handling, and will obtain
Corncob residue is packed into hermetic bag in case follow-up use after processing；
(2) electroplating effluent recycling：Corncob residue after the processing of step (1) is weighed, is put into reaction kettle, adds in sodium sulfite
Then solid adds in the mass concentration of distillation water management stock suspension 10%~30%, stirs so that raw material is mixed with liquid
Close uniform, and it is 1.0~7.0 to adjust the pH of the reaction system with 0.1mol/LNaOH solution, control the temperature of reaction 30~
140℃；
Wherein, the dosage of the sodium sulfite solid is 1.0%~20.0% of corncob residue absolute dry mass after handling；
(3) after reaction reaches 10~120min, collect the corncob residue after reaction and wash repeatedly for several times until being in neutrality
Until, corncob residue after must washing, and place it under 2~10 DEG C of environment of low temperature and store subsequently to use；
(4) enzyme hydrolysis：Corncob residue is put into as enzyme hydrolysis substrate in reaction kettle after weighing washing, adds in HAc-NaAc
Buffer solution makes its mass concentration 2.0%~20.0%, and stirring uniformly disperses raw material；Enzyme solution is added in into reaction kettle again, is made
The dosage of entire hydrolyzation system cellulase is 5~40FPU/g substrates；Distilled water is added into reaction kettle again, control is entire
Enzyme hydrolysis concentration of substrate in enzyme hydrolysis system is 1.0%~15.0% (mass fraction), finally seals bottleneck with preservative film, puts
It is stirred continuously under constant temperature and carries out enzyme hydrolysis processing, obtain hydrolyzate, that is, completing the efficient enzyme hydrolysis production of corncob residue can send out
Ferment sugar；
The condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 45~60 DEG C, hydrolysis time for 20~
120h, rotating speed are 100~500rpm.
Moreover, (1) middle screening is to be screened with 40 mesh to the step.
Moreover, the pH of the step (4) middle HAc-NaAc buffer solutions is 4.5~5.0, preparation method is：It weighs anhydrous
Sodium acetate solid and add in glacial acetic acid make its solid-to-liquid ratio be 1:2~3:1, water is added to be stirred continuously and is allowed to dissolve, controls whole system
PH 4.5~5.0.
Moreover, (4) the concentration of glucose is detected the step using high performance liquid chromatograph in middle hydrolyzate.
Moreover, the concrete operations condition of concentration of glucose is in the high performance liquid chromatograph detection hydrolyzate：Using liquid
Phase pillar is Biorad Aminex HPX-87H column, and 300mm × 7.8mm, mobile phase is 5mmol/L H2SO4, flow velocity is
0.6mL/min, column temperature are 55 DEG C, and sample size is 10 μ L, is detected using Composition distribution, and detector temperature is 55 DEG C.
The advantages of present invention obtains and good effect are：
1. this method utilizes electroplating effluent recycling technical finesse corncob residue, increase the hydrophily of lignin in fibrous raw material
Can, it makes it easier to dissolve out, so as to improve corncob residue enzyme hydrolysis yield；It is high-valued that this method can obtain corncob residue
It utilizes, while reduces the input cost of enzyme hydrolysis, improve enzyme hydrolysis efficiency, and be subsequent processing and utilization or production high added value
Product provides raw material basis, so as to expand the application field of corncob residue and range, and can effectively reduce lumber fibre original
The usage amount of material.
2. the method for the present invention handles corncob residue with electroplating effluent recycling and carried out efficient enzyme hydrolysis, obtain
Higher hydrolysis conversion was obtained, was of great significance in the efficient use aspects of biomass resource.
3. the raw material in the present invention is agricultural wastes --- corncob residue, carrying out biological enzyme hydrolysis as raw material carries
Glucose is taken, reduces the input cost of biological enzyme hydrolysis, reduces the load of environmental pollution, alleviates people for fossil energy
The demand in source can reduce the usage amount of lignocellulose raw material, meet the theory of Green Sustainable.
Under the premise of 4. the method for the present invention reduces enzyme hydrolysis input cost, realize the effectively hydrolyzing saccharification of corncob residue
Purpose, hydrolysis yield is high, has very big economic benefit.
Specific embodiment
With reference to embodiment, the present invention is further described；Following embodiments are illustrative, be not it is limited,
Protection scope of the present invention cannot be limited with following embodiments.
Raw material used in the present invention is conventional commercial product unless otherwise specified；Used in the present invention
Method is the conventional method of this field unless otherwise specified.
Embodiment 1
A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue, step are as follows：
[1] slurry prepares：By corncob residue through air-drying, crushing, screened with 40 mesh, corncob is residual after must handling
Slag, and corncob residue after obtained processing is packed into hermetic bag in case follow-up use；
[2] electroplating effluent recycling：The corncob residue through above-mentioned processing of certain absolute dry mass is weighed, is put into reaction kettle
It is interior, sodium sulfite solid is added in, the mass concentration for then adding in distillation water management stock suspension is 15.0%, is stirred so that former
Material is uniformly mixed with liquid, and it is 2.0 to adjust the pH of the reaction system with 0.1mol/LNaOH solution, and the temperature for controlling reaction is
80℃；
Wherein, the dosage of sodium sulfite solid is 1.0% (mass fraction) of corncob residuum feedstocks absolute dry mass；
[3] after reaction reach 60min after, collect reaction after corncob residue wash repeatedly for several times until be in neutrality for
Only, it and places it under low temperature (2~10) DEG C environment and stores subsequently to use；
[4] enzyme hydrolysis：Corncob residue of certain absolute dry mass Jing Guo above-mentioned processing is weighed to be put into as enzyme hydrolysis substrate
In reaction kettle, adding in HAc-NaAc buffer solutions makes its mass concentration 10.0% or so, and stirring uniformly disperses raw material.Again to anti-
The enzyme solution that certain volume is added in kettle is answered, the dosage for making entire hydrolyzation system cellulase is 11FPU/g substrates.Again to reaction
A certain amount of distilled water is added in kettle, it is 5.0% (mass fraction) to control the enzyme hydrolysis concentration of substrate in entire enzyme hydrolysis system,
Bottleneck finally is sealed with preservative film, is placed under constant temperature and is stirred continuously progress enzyme hydrolysis processing, obtain hydrolyzate, that is, complete corn
The efficient enzyme hydrolysis production fermentable sugars of core residue；
Wherein, the condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 50 DEG C, hydrolysis time 59h, is turned
Speed is 180rpm；
Wherein, the pH of the HAc-NaAc buffer solutions is 4.5~5.0, and preparation method is：Weigh a certain amount of anhydrous second
Sour sodium solid and add in glacial acetic acid make its solid-to-liquid ratio be 4:3, water is added to be stirred continuously and is allowed to dissolve, the pH of whole system is controlled to exist
4.5~5.0 or so；
[5] hydrolysis yield detection：Concentration of glucose in hydrolyzate is detected using high performance liquid chromatograph.
The concrete operations condition of concentration of glucose is in the high performance liquid chromatograph detection hydrolyzate：Using liquid phase pillar
For Biorad Aminex HPX-87H column (300mm × 7.8mm), mobile phase is 5mmol/L H2SO4, flow velocity is
0.6mL/min, column temperature are 55 DEG C, and sample size is 10 μ L, is detected using Composition distribution, and detector temperature is 55 DEG C.
Testing result：Under above-mentioned enzyme hydrolysis condition, a concentration of 23.60g/L of glucose, the grape of substrate in hydrolyzate
Sugared conversion ratio is 70.99%.
Embodiment 2
A kind of method that achievable efficient enzyme hydrolysis of corncob residue can produce fermentable sugars, step are as follows：
[1] slurry prepares：By corncob residue through air-drying, crushing, screened with 40 mesh, corncob is residual after must handling
Slag, and corncob residue after obtained processing is packed into hermetic bag in case follow-up use；
[2] electroplating effluent recycling：The corncob residuum feedstocks through above-mentioned processing of certain absolute dry mass are weighed, are put into reaction
In kettle, add in sodium sulfite solid, then add in the mass concentration of distillation water management stock suspension as 15.0%, stir so that
Raw material is uniformly mixed with liquid, and it is 3.0 to adjust the pH of the reaction system with 0.1mol/LNaOH solution, controls the temperature of reaction
At 80 DEG C；
Wherein, the dosage of sodium sulfite solid is 2.0% (mass fraction) of corncob residuum feedstocks absolute dry mass；
[3] after reaction reach 60min after, collect reaction after corncob residue wash repeatedly for several times until be in neutrality for
Only, it and places it under low temperature (2~10) DEG C environment and stores subsequently to use；
[4] enzyme hydrolysis：Corncob residue of certain absolute dry mass Jing Guo above-mentioned processing is weighed to be put into as enzyme hydrolysis substrate
In reaction kettle, adding in HAc-NaAc buffer solutions makes its mass concentration 10.0% or so, and stirring uniformly disperses raw material.Again to anti-
The enzyme solution that certain volume is added in kettle is answered, the dosage for making entire hydrolyzation system cellulase is 20FPU/g substrates.Again to reaction
A certain amount of distilled water is added in kettle, it is 5.0% (mass fraction) to control the enzyme hydrolysis concentration of substrate in entire enzyme hydrolysis system,
Bottleneck finally is sealed with preservative film, is placed under constant temperature and is stirred continuously progress enzyme hydrolysis processing, obtain hydrolyzate, that is, complete corn
The efficient enzyme hydrolysis production fermentable sugars of core residue；
Wherein, the condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 50 DEG C, hydrolysis time 59h, is turned
Speed is 200rpm；
Wherein, the pH of the HAc-NaAc buffer solutions is 4.5~5.0, and preparation method is：Weigh a certain amount of anhydrous second
Sour sodium solid and add in glacial acetic acid make its solid-to-liquid ratio be 4:3, water is added to be stirred continuously and is allowed to dissolve, the pH of whole system is controlled to exist
4.5~5.0 or so；
[5] hydrolysis yield detection：Concentration of glucose in hydrolyzate is detected using high performance liquid chromatograph.
The concrete operations condition of concentration of glucose is the same as embodiment 1 in the high performance liquid chromatograph detection hydrolyzate.
Testing result：Under above-mentioned enzyme hydrolysis condition, a concentration of 25.85g/L of glucose, the grape of substrate in hydrolyzate
Sugared conversion ratio is 77.74%.
Embodiment 3
A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue, step are as follows：
[1] slurry prepares：By corncob residue through air-drying, crushing, screened with 40 mesh, and obtained raw material is filled
Enter in hermetic bag in case follow-up use；
[2] electroplating effluent recycling：The corncob residue through above-mentioned processing of certain absolute dry mass is weighed, is put into reaction kettle
It is interior, sodium sulfite solid is added in, the mass concentration for then adding in distillation water management stock suspension is 15.0%, is stirred so that former
Material be uniformly mixed with liquid, and with 0.1mol/LNaOH solution adjust the reaction system pH be 4.0, the temperature of reaction is controlled to exist
80℃；
Wherein, the dosage of sodium sulfite solid is 4.0% (mass fraction) of corncob residuum feedstocks absolute dry mass；
[3] after reaction reach 60min after, collect reaction after corncob residue wash repeatedly for several times until be in neutrality for
Only, it and places it under low temperature (2~10) DEG C environment and stores subsequently to use；
[4] enzyme hydrolysis：Corncob residue of certain absolute dry mass Jing Guo above-mentioned processing is weighed to be put into as enzyme hydrolysis substrate
In reaction kettle, adding in HAc-NaAc buffer solutions makes its mass concentration 10.0% or so, and stirring uniformly disperses raw material.Again to anti-
The enzyme solution that certain volume is added in kettle is answered, the dosage for making entire hydrolyzation system cellulase is 15FPU/g substrates.Again to reaction
A certain amount of distilled water is added in kettle, it is 5.0% (mass fraction) to control the enzyme hydrolysis concentration of substrate in entire enzyme hydrolysis system,
Bottleneck finally is sealed with preservative film, is placed under constant temperature and is stirred continuously progress enzyme hydrolysis processing, obtain hydrolyzate, that is, complete corn
The efficient enzyme hydrolysis production fermentable sugars of core residue；
Wherein, the condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 50 DEG C, hydrolysis time 46h, is turned
Speed is 300rpm；
Wherein, the pH of the HAc-NaAc buffer solutions is 4.5~5.0, and preparation method is：Weigh a certain amount of anhydrous second
Sour sodium solid and add in glacial acetic acid make its solid-to-liquid ratio be 4:3, water is added to be stirred continuously and is allowed to dissolve, the pH of whole system is controlled to exist
4.5~5.0 or so；
[5] hydrolysis yield detection：Concentration of glucose in hydrolyzate is detected using high performance liquid chromatograph.
The concrete operations condition of concentration of glucose is the same as embodiment 1 in the high performance liquid chromatograph detection hydrolyzate.
Testing result：Under above-mentioned enzyme hydrolysis condition, a concentration of 27.43g/L of glucose, the grape of substrate in hydrolyzate
Sugared conversion ratio is 82.51%.
Embodiment 4
A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue, step are as follows：
[1] slurry prepares：By corncob residuum feedstocks through air-drying, crushing, screened with 40 mesh, and the original that will be obtained
Material is packed into hermetic bag in case follow-up use；
[2] electroplating effluent recycling：The corncob residuum feedstocks through above-mentioned processing of certain absolute dry mass are weighed, are put into reaction
In kettle, add in sodium sulfite solid, then add in the mass concentration of distillation water management stock suspension as 15.0%, stir so that
Raw material is uniformly mixed with liquid, and it is 2.0 to adjust the pH of the reaction system with 0.1mol/LNaOH solution, controls the temperature of reaction
At 80 DEG C；
Wherein, 8.0% (mass fraction) of the dosage of sodium sulfite solid for the absolute dry mass of corncob residuum feedstocks；
[3] after reaction reach 60min after, collect reaction after corncob residue wash repeatedly for several times until be in neutrality for
Only, it and places it under low temperature (2~10) DEG C environment and stores subsequently to use；
[4] enzyme hydrolysis：Corncob residue of certain absolute dry mass Jing Guo above-mentioned processing is weighed to be put into as enzyme hydrolysis substrate
In reaction kettle, adding in HAc-NaAc buffer solutions makes its mass concentration 20% or so, and stirring uniformly disperses raw material.Again to reaction
The enzyme solution of certain volume is added in kettle, the dosage for making entire hydrolyzation system cellulase is 30FPU/g substrates.Again to reaction kettle
A certain amount of distilled water is inside added, it is 5.0% (mass fraction) to control the enzyme hydrolysis concentration of substrate in entire enzyme hydrolysis system, most
Bottleneck is sealed with preservative film afterwards, is placed under constant temperature and is stirred continuously progress enzyme hydrolysis processing, obtain hydrolyzate, that is, complete corncob
The efficient enzyme hydrolysis production fermentable sugars of residue；
Wherein, the condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 50 DEG C, hydrolysis time 60h, is turned
Speed is 200rpm；
Wherein, the pH of the HAc-NaAc buffer solutions is 4.5~5.0, and preparation method is：Weigh a certain amount of anhydrous second
Sour sodium solid and add in glacial acetic acid make its solid-to-liquid ratio be 4:3, water is added to be stirred continuously and is allowed to dissolve, the pH of whole system is controlled to exist
4.5~5.0 or so；
[5] hydrolysis yield detection：Concentration of glucose in hydrolyzate is detected using high performance liquid chromatograph.
The concrete operations condition of concentration of glucose is the same as embodiment 1 in the high performance liquid chromatograph detection hydrolyzate.
Testing result：Under above-mentioned enzyme hydrolysis condition, after hydrolyzing 60h, a concentration of 28.50g/L of glucose in hydrolyzate,
The inversion rate of glucose of substrate is 85.74%.
Claims (5)
- A kind of 1. method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue, it is characterised in that：Step is as follows：(1) raw material prepares：By corncob residue through air-drying, crushing, screening, corncob residue after must handling, and the processing that will be obtained Corncob residue is packed into hermetic bag in case follow-up use afterwards；(2) electroplating effluent recycling：Corncob residue after the processing of step (1) is weighed, is put into reaction kettle, sodium sulfite is added in and consolidates Then body adds in the mass concentration of distillation water management stock suspension 10%~30%, stirs so that raw material is mixed with liquid Uniformly, it is 1.0~7.0 and to adjust the pH of the reaction system with 0.1mol/LNaOH solution, controls the temperature of reaction 30~140 ℃；Wherein, the dosage of the sodium sulfite solid is 1.0%~20.0% of corncob residue absolute dry mass after handling；After reaction reach 10~120min after, collect reaction after corncob residue wash repeatedly for several times until be in neutrality for Only, corncob residue after must washing, and place it under 2~10 DEG C of environment of low temperature and store subsequently to use；(4) enzyme hydrolysis：Corncob residue is put into as enzyme hydrolysis substrate in reaction kettle after weighing washing, adds in HAc-NaAc bufferings Solution makes its mass concentration 2.0%~20.0%, and stirring uniformly disperses raw material；Enzyme solution is added in into reaction kettle again, is made entire The dosage of hydrolyzation system cellulase is 5~40FPU/g substrates；Distilled water is added into reaction kettle again, controls entire enzyme water Enzyme hydrolysis concentration of substrate in enzymatic hydrolysis system is 1.0%~15.0% (mass fraction), finally seals bottleneck with preservative film, is placed in perseverance It is stirred continuously under the conditions of temperature and carries out enzyme hydrolysis processing, obtain hydrolyzate, that is, complete the efficient enzyme hydrolysis production fermentable sugars of corncob residue；The condition of enzyme hydrolysis processing is under the constant temperature：Hydrolysis temperature is 45~60 DEG C, and hydrolysis time is 20~120h, is turned Speed is 100~500rpm.
- 2. the method for the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue according to claim 1, feature exist In：(1) middle screening is to be screened with 40 mesh to the step.
- 3. the method for the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue according to claim 1, feature exist In：For 4.5~5.0, preparation method is the pH of the step (4) middle HAc-NaAc buffer solutions：Weigh anhydrous sodium acetate solid And adding in glacial acetic acid makes its solid-to-liquid ratio be 1:2~3:1, water is added to be stirred continuously and is allowed to dissolve, control the pH of whole system 4.5~ 5.0。
- 4. the method for the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue according to any one of claims 1 to 3, It is characterized in that：(4) the concentration of glucose is detected the step using high performance liquid chromatograph in middle hydrolyzate.
- 5. the method for the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue according to claim 4, feature exist In：The concrete operations condition of concentration of glucose is in the high performance liquid chromatograph detection hydrolyzate：Use liquid phase pillar for Biorad Aminex HPX-87H column, 300mm × 7.8mm, mobile phase is 5mmol/L H2SO4, flow velocity 0.6mL/ Min, column temperature are 55 DEG C, and sample size is 10 μ L, is detected using Composition distribution, and detector temperature is 55 DEG C.
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| CN201711317723.4A Pending CN108179159A (en) | 2017-12-12 | 2017-12-12 | A kind of method of the efficient enzyme hydrolysis production fermentable sugars of achievable corncob residue |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104830928A (en) * | 2015-05-15 | 2015-08-12 | 江南大学 | Pretreatment method of lignocellulose by using microwave-assisted alkaline sodium sulfite |
| WO2015155788A2 (en) * | 2014-04-07 | 2015-10-15 | Praj Industries Limited | Method for efficient enzymatic hydrolysis of lignocellulosic materials |
| CN105039458A (en) * | 2015-07-28 | 2015-11-11 | 天津科技大学 | Method for realizing clean separation of carbohydrate from lignin components in corncob residue |
| CN105625075A (en) * | 2016-03-09 | 2016-06-01 | 中国科学院青岛生物能源与过程研究所 | Method for pretreatment and separation of lignocellulose biomass |
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2017
- 2017-12-12 CN CN201711317723.4A patent/CN108179159A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015155788A2 (en) * | 2014-04-07 | 2015-10-15 | Praj Industries Limited | Method for efficient enzymatic hydrolysis of lignocellulosic materials |
| CN104830928A (en) * | 2015-05-15 | 2015-08-12 | 江南大学 | Pretreatment method of lignocellulose by using microwave-assisted alkaline sodium sulfite |
| CN105039458A (en) * | 2015-07-28 | 2015-11-11 | 天津科技大学 | Method for realizing clean separation of carbohydrate from lignin components in corncob residue |
| CN105625075A (en) * | 2016-03-09 | 2016-06-01 | 中国科学院青岛生物能源与过程研究所 | Method for pretreatment and separation of lignocellulose biomass |
Non-Patent Citations (1)
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| 姜力凤等: "亚硫酸钠预处理对木糖渣木质素的去除及纤维素酶水解的影响", 《生物加工过程》 * |
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