CN108174819A - It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index - Google Patents
It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index Download PDFInfo
- Publication number
- CN108174819A CN108174819A CN201711423905.XA CN201711423905A CN108174819A CN 108174819 A CN108174819 A CN 108174819A CN 201711423905 A CN201711423905 A CN 201711423905A CN 108174819 A CN108174819 A CN 108174819A
- Authority
- CN
- China
- Prior art keywords
- heat
- rectal temperature
- meat rabbit
- evaluation
- rabbit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000283973 Oryctolagus cuniculus Species 0.000 title claims abstract description 83
- 235000013372 meat Nutrition 0.000 title claims abstract description 53
- 238000011156 evaluation Methods 0.000 title claims abstract description 23
- 238000003556 assay Methods 0.000 title claims description 12
- 230000008642 heat stress Effects 0.000 claims abstract description 54
- 238000000034 method Methods 0.000 claims abstract description 10
- 230000007613 environmental effect Effects 0.000 claims abstract description 8
- 230000000977 initiatory effect Effects 0.000 claims abstract description 3
- 230000035790 physiological processes and functions Effects 0.000 claims abstract description 3
- 238000012545 processing Methods 0.000 claims description 8
- 230000002596 correlated effect Effects 0.000 claims description 7
- 241000283977 Oryctolagus Species 0.000 claims description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims description 3
- 229910052753 mercury Inorganic materials 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 description 12
- 230000035882 stress Effects 0.000 description 12
- 238000009395 breeding Methods 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 7
- 230000036760 body temperature Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000010219 correlation analysis Methods 0.000 description 5
- 238000005286 illumination Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 210000000664 rectum Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 210000000436 anus Anatomy 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 238000013499 data model Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 206010019332 Heat exhaustion Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 230000006461 physiological response Effects 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 210000000106 sweat gland Anatomy 0.000 description 1
- 230000028016 temperature homeostasis Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention belongs to cultural technique field more particularly to a kind of refer to object detection method by the use of rectal temperature as meat rabbit Evaluation of Heat Tolerance.Meat rabbit is placed in pretreatment more than 5d under thermophilic environmental condition by the present invention, is consistent all individual initiating physiological states；Pretreated meat rabbit is placed under high ambient conditions again, the rectal temperature of individual is measured during heat stress 8h.Individual rectal temperature is with heat-resistant time in extremely notable negative correlation, and rectal temperature more low heat resistant can be better, and rectal temperature more high heat resistance is poorer.The index can reflect the height of individual heat resistance well, selection index, and simple and quick measure can be provided for the cultivation of meat rabbit heat-resistant strain, stability is high, accurately.
Description
Technical field
The invention belongs to cultural technique fields more particularly to a kind of by the use of rectal temperature as meat rabbit Evaluation of Heat Tolerance index
Assay method.
Background technology
China is the meat rabbit cultivation big country of the first in the world, but due to being located in subtropical zone, summer universal high temperature, and meat rabbit is complete
Body hair and almost without functional sweat gland, is difficult to eliminate heat extra in vivo when environment temperature is higher, be very easy to out
Existing heat stress, leads to internal metabolic imbalance so that feed intake declines, the price of deed reduces, under the speed of growth and Abwehrkraft des Koepers
Drop, the reduction of male and female rabbit reproductive performance etc., are the major reasons for causing Industry losses.
Although people are in effective measures (such as rabbit studied with rabbit heat stress is explored to some alleviations or avoided in production practices
Give up facilities and equipment transformation, production technology improvement, nutrition regulation and chemoprophylaxis etc.), but still cannot thoroughly solve the problems, such as.Research hair
Existing, in torrid areas, rabbit also can successfully be raised under the conditions of being in 31 DEG C~35 DEG C, and it is refrigerant to have adapted to U.S.'s northwest Pacific
The rabbit of environment may then die of heat stress under conditions of the temperature occurred once in a while is more than 32 DEG C.It is it is moreover found that different new
There is significant differences for the heat resistance of western blue rabbit individual, illustrate that rabbit is restricted hot tolerance by inherent cause, may
It is a quantitative character, this allows for, by cultivating heat-resisting meat rabbit strain to improve rabbit itself heat resistance, being inherently eliminated rabbit
Heat stress be possibly realized.And to carry out the cultivation of meat rabbit heat-resistant strain, first have to the reliable and simple criterion for breeding easily surveyed.
The time (heat-resistant time) that animal survives under high temperature environment is the direct standard for judging its heat resistance, but heat-resistant time index
Without actual application value.
Meat rabbit is homeothermal animal, and body heat balance is kept by adjusting itself heat production and heat dissipation, and holding body temperature is stablized relatively,
Normal range (NR) is generally between 38.5 DEG C~39.5 DEG C.It is not enough to maintain body heat when environment temperature is increased to internal heat regulatory mechanisms
During balance, there are a series of physiological responses to heat stress until heat exhaustion is dead in the body temperature raising of meat rabbit.Rectal temperature is often used as weighing
The physical signs of animal heat resistance, because the strong animal of heat resistance is under high temperature environment, Thermoregulation ability is stronger, rectal temperature
It maintains normal range (NR) or elevation amplitude is small.To ox, sheep etc. research shows that, under the conditions of heat stress with low rectal temperature
Individual heat resistance is stronger, therefore is widely used as Holstein Cattle, and genetic force is higher, and calculated by rectal temperature
Heat-resistance coefficient is utilized as the index of evaluation ox heat resistance.To chicken research, it was also found that find 35 DEG C at heat stress 18h it is straight
The changing value of intestines temperature and starting rectal temperature is the important evaluation index of chicken heat resistance.And on rabbit, there has been no corresponding
Evaluation of Heat Tolerance index, the cultivation of meat rabbit heat-resistant strain are not known where to begin more.
Invention content
In view of the above-mentioned problems, the present invention provide it is a kind of by the use of rectal temperature as the measure side of meat rabbit Evaluation of Heat Tolerance index
Method, the cultivation for meat rabbit heat-resistant strain provide selection index, and method is simple, easy to operate, and method accurate stable, quick to measure.
It is a kind of by the use of rectal temperature as meat rabbit Evaluation of Heat Tolerance index in the present invention of solution more than technical problem
Detection method, which is characterized in that include the following steps：
(1) it pre-processes：Meat rabbit is placed in pretreatment more than 5d under thermophilic environmental condition, makes all individual initiating physiological states
It is consistent；
Pretreatment can allow all rabbit physiological status consistent, if not pre-processing the difference that can cause individual itself, in advance
Processing time is too short, and the effect of normalizing is not achieved；Pretreatment temperature is excessively high, then the mean temperature after normalizing can be higher, pretreatment
Temperature is too low, then on the contrary.
Pretreatment makes physiological status normalizing in the present invention, and measurement result below is not influenced by season.In Various Seasonal
The heat stress processing of rabbit will be different, and the body temperature of summer entirety can be higher than winter, as long as there is a normalized to compare
Compared with not influencing the detection of result.
(2) heat stress processing and thermometric：Pretreated meat rabbit is placed under high ambient conditions, when heat stress 8h measures
The rectal temperature of individual；
When detecting rabbit rectum temperature during the difference of position, it can have a certain impact to result.
As soon as body temperature is exactly the thermally equilibrated reflection of a body, individual represents it in fact to the balance of heat production and heat-sinking capability
The height of heat resistance itself with heat stress handles immeasurablel thermal balance ability to become to measure in of the invention
Determining value.
(4) Evaluation of Heat Tolerance:Rectal temperature is with the heat stress time in extremely significantly negative correlation, rectal temperature get over low heat resistant energy
Better, rectal temperature more high heat resistance is poorer.
Mathematical analysis model is established using the curvilinear regression program in SPSS17.0 softwares：Using rectal temperature as change certainly
Amount, heat-resistant time establish data model as dependent variable：Y=5.142E+16EXP (- 0.75x).
The thermophilic is 18-22 DEG C.
The thermophilic is 21 DEG C ± 1 DEG C.
The thermophilic is preferably 21 DEG C, natural humidity and illumination.
The high temperature is 35 DEG C -39 DEG C, continuous light, intensity of illumination >=100lux.
Preferred high temperature is 38 DEG C in the high ambient conditions, humidity >=80%.
The rectal temperature for measuring individual meat rabbit is measured using mercury or electronic thermometer.
The meat rabbit is New Zealand rabbit.
The New Zealand's kind selected in the present invention is the general kind in a world, and experiment material easily obtains.
Rabbit is the standby rabbit at 4 monthly ages in the present invention, is freely eaten and drinks water.Rabbit weight in measure is the bigger the better or rabbit
Age is the bigger the better, be conveniently operated, reduce measure rectal temperature when damage.
Description of the drawings
Fig. 1 is change curve of the rectal temperature with the heat stress time.
Fig. 2 is the changing value of rectal temperature with the change curve of heat stress time
Fig. 3 is the relationship of heat-resistant time and rectal temperature
Specific embodiment
The present invention is further described with specific embodiment with reference to the accompanying drawings of the specification：
Embodiment 1
Refer to object detection method by the use of rectal temperature as meat rabbit Evaluation of Heat Tolerance, be as follows：
(1) meat rabbit to be detected is placed in more than 5d under the environmental condition of 18 DEG C of pretreatment house for New Zealand rabbit individual,
Natural humidity and illumination；
(2) heat stress house environment temperature is increased to 37 DEG C, and remain stable；
By the meat rabbit individual heat of transfer to be measured of pretreatment stress room, stress be taken out after 8h, measure rectal temperature；
(3) Evaluation of Heat Tolerance：Rectal temperature is with the heat stress time in extremely significantly negative correlation, rectal temperature get over low heat resistant energy
Better, rectal temperature more high heat resistance is poorer.The index can reflect the height of individual heat resistance well, can be meat
The cultivation of rabbit heat-resistant strain provides selection index.
Utilize the curvilinear regression program founding mathematical models in SPSS17.0 softwares：It is resistance to using rectal temperature as independent variable
The hot time establishes data model as dependent variable：Y=5.142E+16EXP (- 0.75x).
The rectal temperature for measuring individual meat rabbit is measured using mercury or electronic thermometer, rectal temperature assay method
For：The probe of electronic thermometer or mercurial thermometer are inserted into rectum 5cm depths from meat rabbit anus, measure is averaged afterwards three times
Value, precision are 0.01 DEG C.
Rectal temperature is measured, because rectal temperature can stablize reaction body temperature, is influenced by ambient temperature small.But rectum has
Its natural length, closer to anus, temperature is lower and bigger by ectocine, and more inward, temperature is more stable, but position again not
Can too inside, one is because the temperature measuring result difference of the inside is little, secondly thermometer is inserted to enter so much, second is that
Easy damage rectal causes bleeding.
Embodiment 2
Other contents such as embodiment 1, wherein pretreatment is more than the 5d under the environmental condition of 22 DEG C of thermophilic, natural humidity and
Illumination；Temperature is increased to 39 DEG C in high ambient conditions, and remains stable；It stress by the meat rabbit individual heat of transfer to be measured of pretreatment
Room stress take out after 8h, measure rectal temperature；Rectal temperature is with the heat stress time in extremely significantly negative correlation, rectal temperature are lower
Heat resistance is better, and rectal temperature more high heat resistance is poorer.
Embodiment 3
Other contents such as embodiment 1, wherein pretreatment is more than the 5d under the environmental condition of 21 DEG C of thermophilic；Hot environment item
Temperature is increased to 38 DEG C in part, and remains stable；Humidity >=80%, continuous light, intensity of illumination >=100lux；By pretreatment
Meat rabbit individual heat of transfer to be measured stress room, stress be taken out after 8h, measure rectal temperature；Rectal temperature is with the heat stress time in extremely aobvious
Negative correlation is write, rectal temperature more low heat resistant can be better, and rectal temperature more high heat resistance is poorer.
Using rectal temperature of the meat rabbit under 38 DEG C of hot conditions after heat stress 8h as the evaluation index of meat rabbit heat resistance, heat
Stress 8h when the size of rectal temperature that is measured and individual heat-resistant time in extremely significantly negatively correlated, lower of rectal temperature
Body heat-resistant time is longer.
Embodiment 4
Other contents such as embodiment 1, wherein pretreatment is more than the 5d under the environmental condition of 20 DEG C of thermophilic；Hot environment item
Temperature is increased to 35 DEG C in part, and remains stable；By the meat rabbit individual heat of transfer to be measured of pretreatment stress room, stress be taken after 8h
Go out, measure rectal temperature；Rectal temperature is in extremely significantly negatively correlated with the heat stress time, and rectal temperature more low heat resistant can be better,
Rectal temperature more high heat resistance is poorer.The index can reflect the height of individual heat resistance well, can be that meat rabbit is resistance to
The cultivation of hot strain provides selection index.
Embodiment 5
Other contents such as embodiment is, wherein pretreatment is more than the 5d under the environmental condition of 19 DEG C of thermophilic；Hot environment
Temperature is increased to 36 DEG C in condition, and remains stable；By the meat rabbit individual heat of transfer to be measured of pretreatment stress room, stress be taken after 8h
Go out, measure rectal temperature；Rectal temperature is in extremely significantly negatively correlated with the heat stress time, and rectal temperature more low heat resistant can be better,
Rectal temperature more high heat resistance is poorer.The index can reflect the height of individual heat resistance well, can be that meat rabbit is resistance to
The cultivation of hot strain provides selection index.
Experiment one
Influences of the high temperature heat stress 8h to meat rabbit rectal temperature and related to heat-resistant time
Experimental animal processing
The same period is selected to be born, healthy 4 monthly age new zealand rabbit 55 similar in weight, be randomly assigned in 2 artificial temperature controlled compartments
It is interior, and real time humiture is measured using intelligent temperature and humidity recorder (APRESYS, 179-TH).Wherein thermophilic group (N=25, buck
11, female rabbit 14) using air-conditioning temperature-reducing, manual humidification, environment temperature is 21 ± 1 DEG C；High temperature heat stress group (N=30, male and female
It is fifty-fifty) it is heated using warm wind, temperature automatically controlled, manual humidification, environment temperature is 38 ± 1 DEG C.Pre-feeding period 5 days, all rabbits are only located
In thermophilic environment.Experiment is continued until that all rabbits of high temperature group are only dead.Free water and feeding during entire processing, raising
Management condition is consistent (daily ration Major Nutrient level is crude protein 16.0%, crude fibre 14.0%, digestible energy 10.50MJ/kg).
Assay method
(heat stress before being put into using electronic thermometer for animals (32 DEG C~44 DEG C of measurement range, 0.01 DEG C of resolution ratio) measure
The rectal temperature of high temperature group and all individuals of thermophilic group when 0h) and being put into rear 8h (heat stress 8h), and accurate recording high temperature group
In every rabbit death time, start to calculate every rabbit time-to-live in a high temperauture environment for starting point with formal test, i.e.,
The heat-resistant time of rabbit.
Statistical analysis
Variance analysis is carried out using general linear model (GLM) program in SPSS17.0 softwares, as a result uses least square
Means standard deviation represents.Simultaneously using in software simple correlation analysis model (CORR) program carry out rectal temperature and it is heat-resisting when
Between correlation analysis.
Result of the test
As a result as shown in table 1,2：When heat stress originates (0h), the rectal temperature of two groups of rabbits only tests 8h without significant difference
Afterwards, the rectal temperature of heat stress group individual extremely significantly increases 1.68 DEG C, and thermophilic group individual rectal temperature is still without significance difference
It is different, fully show that heat stress 8h poles significantly affect the rectal temperature of meat rabbit.The rectal temperature and heat-resistant time of heat stress group individual
Correlation analysis find that rectal temperature during 0h and heat-resistant time are without significantly correlated, rectal temperature and heat during heat stress 8h
Stress 0~8h rectal temperature changing value with heat-resistant time in extremely significantly negatively correlated, show rectal temperature during heat stress 8h
The low individual heat resistance of low individual or rectal temperature lift-off value is more preferable.The rectal temperature of heat stress 8h and the phase of heat-resistant time
The absolute value of relationship number, and while measuring the index only need to measure the rectal temperature at 1 time point, and heat stress 0~8 hour
Changing value index needs to measure the rectal temperature at 2 time points, therefore uses the rectal temperature index of heat stress 8h as meat rabbit
Evaluation of Heat Tolerance index is more preferably.
Influence of 1 heat stress of table to rectal temperature
Note：Mark lowercase letter indication difference significantly (P<0.05), mark capitalization represents difference extremely significantly (P<
0.01), similarly hereinafter.
2 rectal temperature of table is related to heat-resistant time
Note：" * " is marked as correlation significantly (P<0.05), mark " * * " is correlation extremely significantly (P<0.01), similarly hereinafter.
Experiment two
The changing rule of Heat-temperature stress initial stage meat rabbit rectal temperature and related to heat-resistant time
Experimental animal processing
Experiment selects that same period birth, healthy 4 monthly ages are newly western similar in weight in the kind warren of Sichuan Province Animal Husbandry Institute of Science and Technology
Blue rabbit 30, male and female is fifty-fifty, is placed in pre- raise in house and raises 10d in advance under the conditions of 21 DEG C ± 1 DEG C.It is transferred to and is had warmed up to 38 after pre- feeding
In DEG C ± 1 DEG C of heat stress room.Real time humiture is measured using intelligent temperature and humidity recorder (APRESYS, 179-TH).Entire place
Free water and feeding during reason, feeding and management condition is consistent, and (daily ration Major Nutrient level is crude protein 16.0%, crude fibre
14.0%, digestible energy 10.50MJ/kg).
Assay method
Using electronic thermometer for animals (32 DEG C~44 DEG C of measurement range, 0.01 DEG C of resolution ratio) measure heat stress 0h, 2h,
4h, 6h and 8h rabbit only rectal temperature, and in accurate recording heat stress group every rabbit death time, with formal test start for
The heat-resistant time of starting point calculates every rabbit time-to-live, i.e. rabbit in a high temperauture environment.
Statistical analysis
Variance analysis is carried out using general linear model (GLM) program in SPSS17.0 softwares, as a result uses least square
Means standard deviation represents.Simultaneously using in software simple correlation analysis model (CORR) program carry out rectal temperature and it is heat-resisting when
Between correlation analysis.
Result of the test
As a result as shown in Figure 1, 2, acute heat stress processing time influences rectal temperature extremely significantly (P<0.01), heat stress
Rectal temperature extremely significantly increases during 2h, then tends towards stability, and rectal temperature variation is not notable during 2~8h of heat stress, very
It is slightly reduced in 6h, body temperature reaches highest, but not notable with heat stress 2h differences during heat stress 8h.With the phase of heat-resistant time
It closes analysis shows (table 3), the rectal temperature or rectal temperature changing value and heat-resistant time of heat stress 2h~8h is in notable or pole
Significantly negatively correlated, the correlation coefficient r value of the wherein rectal temperature of heat stress 8h and heat-resistant time is maximum, is -0.733.
The rectal temperature for obtaining identical conclusion namely heat stress 8h with experiment two from above experiment one is best reflection
The index of the heat resistance of individual.
The different heat stress time rectal temperatures of table 3 or its changing value are related to heat-resistant time
Curve matching
Data source
Individual rectal temperature and corresponding heat-resistant time data source are in 58 rabbits of above-mentioned 2 experiments.
Method
It is carried out curve fitting using the Regression Model of curve in SPSS17.0 softwares.
As a result
It is found by fitting result, the fitting effect of exponential model is best, and best fit equation result is：
Y=5.142E+16EXP (- 0.75x)
The significance test of Regression Equations the result shows that：F=130.542, the level of signifiance 0.000, degree of fitting R2
It is 0.700, from the regression equation illustraton of model of Fig. 3 it can also be seen that models fitting degree is preferable.
Selection and breeding are verified
Selection and breeding underlying group source
Selection and breeding underlying group derives from 58 rabbits of above-mentioned 2 experiments.
Selection
It is set out with practical selection and breeding, according to the size of selection intensity, verifies that the rectal temperature using heat stress 8h is resistance to as meat rabbit
The selection and breeding effect of heating index.And reserve seed for planting rate (initial breeding population) as a control group with 100%, carry out reserve seed for planting group and selection and breeding underlying group
Heat-resistant time carry out one-way analysis of variance, mark " * " be significant difference (P<0.05), mark " * * " is difference extremely significantly (P
<0.01)。
Selection and breeding result
Table 4 is directed to the result of rectal temperature selection and breeding heat resistance
By result (table 4) it is found that choosing seeds for the rectal temperature of meat rabbit individual heat stress 8h, under the rate of reserving seed for planting
Drop, the selection difference of heat-resistant time gradually increase, and the heat-resistant time for being selected in individual is gradually increasing, and reached with the difference of selection and breeding underlying group
To the notable or pole level of signifiance, show that the rectal temperature by the individual heat stress 8h of selection is and then the heat resistance of raising individual
Effectively, meet general character Rules of Breeding New.Therefore, method of the invention is correct.
Claims (8)
1. it is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index, include the following steps：
Pretreatment：Meat rabbit is placed in pretreatment more than 5d under thermophilic environmental condition, all individual initiating physiological states is made to keep one
It causes；
Heat stress processing and thermometric：Pretreated meat rabbit is placed under high ambient conditions, individual is measured during heat stress 8h
Rectal temperature；
Evaluation of Heat Tolerance：Individual rectal temperature is in extremely significantly negatively correlated with heat-resistant time, and rectal temperature more low heat resistant can be better,
Rectal temperature more high heat resistance is poorer.
2. it is according to claim 1 a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index,
It is characterized in that：The thermophilic is 18-22 DEG C.
3. it is according to claim 2 a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index,
It is characterized in that：The thermophilic is 21 DEG C ± 1 DEG C.
4. it is according to claim 3 a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index,
It is characterized in that：The thermophilic is 21 DEG C.
It is 5. a kind of by the use of rectal temperature as the survey of meat rabbit Evaluation of Heat Tolerance index according to any one of claim 1-4
Determine method, it is characterised in that：The high temperature is 35 DEG C -39 DEG C.
It is 6. a kind of by the use of rectal temperature as the measure of meat rabbit Evaluation of Heat Tolerance index according to any one of claim 5
Method, it is characterised in that：The high temperature is 38 DEG C, humidity >=80%.
7. it is according to claim 1 a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index,
It is characterized in that：The rectal temperature for measuring individual meat rabbit is measured using mercury or electronic thermometer.
8. according to the assay method described in any one of claim 1 by the use of rectal temperature as meat rabbit Evaluation of Heat Tolerance index,
It is characterized in that：The meat rabbit is New Zealand rabbit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711423905.XA CN108174819A (en) | 2017-12-25 | 2017-12-25 | It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711423905.XA CN108174819A (en) | 2017-12-25 | 2017-12-25 | It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108174819A true CN108174819A (en) | 2018-06-19 |
Family
ID=62547380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711423905.XA Pending CN108174819A (en) | 2017-12-25 | 2017-12-25 | It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108174819A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114831076A (en) * | 2022-05-19 | 2022-08-02 | 华中农业大学 | Method and device for judging heat resistance of chicken |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423872A (en) * | 2008-12-15 | 2009-05-06 | 南京农业大学 | Molecular marker of holstein cattle heat resistant stress breeding and use thereof |
| CN102125183A (en) * | 2011-01-14 | 2011-07-20 | 河南农业大学 | Beef cattle heat stress resisting feed additive and using method thereof |
| CN102524165A (en) * | 2011-12-23 | 2012-07-04 | 山东农业大学 | Heat-stress-resistant broiler chicken feeding method |
-
2017
- 2017-12-25 CN CN201711423905.XA patent/CN108174819A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101423872A (en) * | 2008-12-15 | 2009-05-06 | 南京农业大学 | Molecular marker of holstein cattle heat resistant stress breeding and use thereof |
| CN102125183A (en) * | 2011-01-14 | 2011-07-20 | 河南农业大学 | Beef cattle heat stress resisting feed additive and using method thereof |
| CN102524165A (en) * | 2011-12-23 | 2012-07-04 | 山东农业大学 | Heat-stress-resistant broiler chicken feeding method |
Non-Patent Citations (2)
| Title |
|---|
| 李丛艳等: "肉兔耐热性评价指标的初步研究", 《中国畜牧杂志》 * |
| 鲁琳等: "《奶牛环境与疾病》", 31 August 2014, 中国农业大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114831076A (en) * | 2022-05-19 | 2022-08-02 | 华中农业大学 | Method and device for judging heat resistance of chicken |
| CN114831076B (en) * | 2022-05-19 | 2023-01-13 | 华中农业大学 | Method and device for judging heat resistance of chicken |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vleck | Energetic cost of incubation in the zebra finch | |
| Drent et al. | Thermoregulatory responses of the Peruvian penguin, Spheniscus humboldti | |
| Pinshow et al. | Energy expenditure for thermoregulation and locomotion in emperor penguins | |
| CN104545823A (en) | Method and device for measuring core body temperature of livestock | |
| Vitt et al. | HOBOs, Tidbits and lizard models: the utility of electronic devices in field studies of ectotherm thermoregulation | |
| Hulbert et al. | The effects of incubation temperature and experimental design on heart rates of lizard embryos | |
| Barroso et al. | Assessing the reliability of thermography to infer internal body temperatures of lizards | |
| Shu et al. | Recent advances on early detection of heat strain in dairy cows using animal-based indicators: A review | |
| Gallego et al. | A protocol for analysing thermal stress in insects using infrared thermography | |
| Ben-Ezra et al. | Are locomotor performances coadapted to preferred basking temperature in the Northern Map Turtle (Graptemys geographica)? | |
| CN108174819A (en) | It is a kind of by the use of rectal temperature as the assay method of meat rabbit Evaluation of Heat Tolerance index | |
| Farahiyah et al. | Design Prototype of Temperature and Humidity Control and Monitoring on Weaver Ant Cage based on Internet of Things | |
| Aerts et al. | A virtual chicken for climate control design: static and dynamic simulations of heat losses | |
| Srivastava et al. | Appropriate THI model and its threshold for goats in semi-arid regions of India | |
| Lees | Biological responses of feedlot cattle to heat load | |
| Hanna et al. | Critical thermal maximum of the green lynx spider, Peucetia viridans (Araneae, Oxyopidae) | |
| Wathes et al. | Sensible heat transfer from the fowl: radiative and gonvegtive heat losses from a flock of broiler chickens | |
| Clark | Consequences of homeothermic capacity of nestlings on parental care in the European starling | |
| Kim et al. | Changes in facial surface temperature of laying hens under different thermal conditions | |
| Newberry et al. | Urban rooftop-nesting Common Nighthawk chicks tolerate high temperatures by hyperthermia with relatively low rates of evaporative water loss | |
| CN104813980B (en) | Non-invasive physical index for evaluating thermal comfort of broiler chicken and application thereof | |
| CN103583468B (en) | Method for rapidly evaluating corn DDGS fowl metabolic energy through physicochemical indexes | |
| CN207322350U (en) | For marking the electronic identity recognizer of live-bird | |
| Abigaba et al. | Suitability of Inguinal and Axillary Sites for Temperature Measurement Using Digital Thermometers: A Comparison with Rectal Thermometry in Broiler Chicken | |
| Green et al. | Calibration and sensitivity analysis of a model of the growing pig for weight gain and composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180619 |