CN108169208A - A kind of Raman spectrum detector calibration method - Google Patents
A kind of Raman spectrum detector calibration method Download PDFInfo
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- CN108169208A CN108169208A CN201810046148.7A CN201810046148A CN108169208A CN 108169208 A CN108169208 A CN 108169208A CN 201810046148 A CN201810046148 A CN 201810046148A CN 108169208 A CN108169208 A CN 108169208A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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Abstract
A kind of Raman spectrum detector calibration method, including following operating procedure, step 1 obtains reference spectra S1；Step 2 obtains initial spectrum S2；Step 3 obtains current background spectrum S3；Step 4, calculate the first difference spectrum Sd1 of the reference spectra S1 and the initial spectrum S2, the second difference spectrum Sd2 of the reference spectra S1 and current background spectrum S3 are calculated, calculates the third difference spectrum Sd3 of the initial spectrum S2 and the current background spectrum S3；Step 5 determines the first offset △ v1 and the second offset △ v2；Step 6 is modified the Raman spectrum of testing result using the first offset △ v1 or the Raman spectrum of testing result is modified using the 4th offset △ v4.The present invention possesses calibration with reference to the advantages of more, calibration degree is high, and real-time is good.
Description
Technical field
The present invention relates to Raman spectrum detection technique fields, and in particular to a kind of Raman spectrum detector calibration method.
Background technology
The wavelength of the laser of Raman spectrometer is typically when laser dispatches from the factory or when Raman spectrometer dispatches from the factory into rower
Location survey amount has determined that.But the wavelength of laser can be because small drift occurs for environment temperature, the fluctuation etc. of electronic circuit.Using
Laser on Raman spectrometer is usually higher to the stability requirement of optical maser wavelength, it is desirable that ± 2cm-1 or higher are stablized
Property parameter directly affects the Raman shifts measurement accuracy of instrument.In long-time service, environmental change, long-distance after Raman spectrometer manufacture
Its optical maser wavelength is waited to be likely to occur drift after transport, it will usually be modified by modes such as self calibrations to optical maser wavelength.
Common method for self-calibrating is：Measure the Raman spectrum of certain standard substance (such as polystyrene), and with the mark of the standard substance
Quasi- spectrogram is compared, and then learns the offset of optical maser wavelength.The shortcomings that existing self-alignment method, is do not have
Real-time can only give a constant correction value, and self-alignment condition is carried out under what state and is difficult to hold.
Invention content
The object of the present invention is to provide a kind of Raman spectrum detector calibration methods, and present invention offer is a kind of quickly to be carried out
The method for carrying out field calibration.
The present invention above-mentioned technical purpose technical scheme is that：A kind of Raman spectrum detector school
Quasi- method, including following operating procedure,
Step 1 carries out standard reference material using Raman spectrum detector the signal sampling of T time section, obtains reference light
Compose S1；
Step 2 using Raman spectrum detector to being carried out the signal sampling of the T time section by object to be detected, obtains just
Beginning spectrum S2；
Step 3 carries out vacant sample cell using Raman spectrum detector the signal sampling of the T time section, obtains
Current background spectrum S3；
Step 4 calculates the first difference spectrum Sd1 of the reference spectra S1 and the initial spectrum S2, calculates the ginseng
The second difference spectrum Sd2 of spectrum S1 and current background spectrum S3 are examined, calculates the initial spectrum S2 and the current background light
Compose the third difference spectrum Sd3 of S3；
Step 5 determines that reference laser is composed according to the second difference spectrum Sd2 and the third difference spectrum Sd3 respectively
Peak with by object laser spectral peak to be checked, respectively according to the center of the reference laser spectral peak and the object laser spectral peak to be checked point
It Que Ding not the first offset △ v1 and the second offset △ v2；
Step 6, the first offset △ v1 and the second offset △ v2, using described the if equal
One offset △ v1 are modified the Raman spectrum of testing result；It is true according to the first difference spectrum Sd1 if unequal
Determine difference laser spectral peak, third offset △ v3 are then determined according to the center of the difference laser spectral peak, using described
Third offset △ v3 are modified to obtain the 4th offset △ v4 to the offset △ v1, finally using the described 4th offset
Amount △ v4 are modified the Raman spectrum of testing result.
The first offset △ v1 will be used to join the standard as a preference of the present invention, the step 5 further includes
The testing result vf for examining object is directly modified, and the 5th offset is obtained after correction result and standard Raman spectroscopy are compared
△ v5 are measured, the first offset △ v1 and the second offset △ v2 are modified using the 5th offset △ v5
Obtain final first offset △ v1aWith final second offset △ v2a, use the final first offset △ v1aWith it is described
Final second offset △ v2aStep 6 behaviour is carried out after replacing the first offset △ v1 and the second offset △ v2
Make.
As a preference of the present invention, the optical density parameter of the notch filtering light piece of the Raman spectrum detector for 6.5~
7.5。
As a preference of the present invention, first it is placed in dark place before being additionally included in the operation for carrying out the step one~step 6
The step 1~step 6 is inside first carried out in advance carries out preposition calibration.
As a preference of the present invention, one~step 6 of the step of preposition calibration is further included and is provided using standard incandescent lamp
Bias light has carried out luminous environment preliminary calibration.
As a preference of the present invention, when the second offset △ v2 are 0 value, without correcting in the step six
Directly export result.
As a preference of the present invention, when the first offset △ v1 are 0 value, directly using described in the step 6
Second offset △ v2 are modified the Raman spectrum of testing result.
As a preference of the present invention, the standard reference material is polystyrene.
In conclusion the present invention has the advantages that：
The present invention possesses calibration with reference to the advantages of more, calibration degree is high, and real-time is good.
Description of the drawings
Fig. 1 is the operational flowchart of the embodiment of the present invention.
Specific embodiment
The present invention is described in further detail below in conjunction with attached drawing.
As shown in Figure 1, the first embodiment of the present invention includes following operating procedure,
Step 1 carries out standard reference material using Raman spectrum detector the unified testing time T time section of standard
Signal sampling obtains reference spectra S1, and wherein standard reference material is polystyrene, the notch filtering light piece of Raman spectrum detector
Optical density parameter is 6.5~7.5；
Step 2, using Raman spectrum detector to by object to be detected carry out T time section signal sampling, obtain initial light
Compose S2；
Step 3 carries out vacant sample cell using Raman spectrum detector the signal sampling of T time section, obtains current
Background spectrum S3；
Step 4 calculates the first difference spectrum Sd1 of reference spectra S1 and initial spectrum S2, calculates reference spectra S1 with working as
The second difference spectrum Sd2 of preceding background spectrum S3 calculates the third difference spectrum of initial spectrum S2 and current background spectrum S3
Sd3；
Step 5 determines reference laser spectral peak with being treated according to the second difference spectrum Sd2 and third difference spectrum Sd3 respectively
Object laser spectral peak is examined, double sampling mistake is determined according to the center of reference laser spectral peak and object laser spectral peak to be checked respectively respectively
The first offset △ v1 and second between the reference laser wavelength that optical maser wavelength is provided with respect to Raman spectrum detector in journey are inclined
Shifting amount △ v2；
Step 6 compares the first offset △ v1 and the second offset △ v2, and the first offset △ v1 are used if equal
The Raman spectrum of testing result vi is modified, correcting mode is vi=vi+ △ v1；According to the first difference light if unequal
Spectrum Sd1 determines difference laser spectral peak, then determines laser in reference substance sampling process according to the center of difference laser spectral peak
Wavelength is with respect to the third offset △ v3 between optical maser wavelength in checking matter sampling process, using third offset △ v3 to offset
Amount △ v1 are modified to obtain the 4th offset △ v4, finally using the 4th offset △ v4 to the Raman spectrum of testing result into
Row is corrected, and correcting mode is vi=vi+ △ v4, in this step when the second offset △ v2 are 0 value, without repairing in step 6
Just directly export result.When the first offset △ v1 are 0 value, directly detection is tied using the second offset △ v2 in step 6
The Raman spectrum of fruit vi is modified.
Second of embodiment of the present invention is under the premise of all operationss step for keeping the first embodiment in step 5
Increase operation again, the operating method is will directly repair the testing result vf of standard reference material using the first offset △ v1
Just, and by correction result and standard Raman spectroscopy it is compared, normalized Raman of the standard Raman spectroscopy for theoretically polystyrene
Spectrum obtains the 5th offset △ v5, using the 5th offset △ v5 to the first offset △ v1 and the second offset more afterwards
△ v2 are modified to obtain final first offset △ v1aWith final second offset △ v2a, use final first offset △
v1aWith final second offset △ v2aStep 6 operation is carried out after replacing the first offset △ v1 and the second offset △ v2.
The embodiment of the present invention one is first placed in dark place in advance with embodiment two before the operation for carrying out step 1~step 6
It first carries out step 1~step 6 and carries out preposition calibration, the purpose of preposition calibration is that occur to eliminate manufacture before routine testing
Error, preposition calibration must assure that △ v1~△ v5 are 0 value.The step of preposition calibration, one~step 6 was further included using warp
Integrating sphere is crossed to carry out optical parameter test and reach standard sources required standard incandescent lamp providing at the beginning of bias light carried out luminous environment
Step calibration, the step in order to generate that the offset under a standard luminous environment dispatches from the factory have luminous environment calibration.
This specific embodiment is only explanation of the invention, is not limitation of the present invention, people in the art
Member can as needed make the present embodiment the modification of no creative contribution after this specification is read, but as long as at this
It is all protected in the right of invention by Patent Law.
Claims (8)
1. a kind of Raman spectrum detector calibration method, it is characterised in that：Including following operating procedure,
Step 1 carries out standard reference material using Raman spectrum detector the signal sampling of T time section, obtains reference spectra
S1；
Step 2 using Raman spectrum detector to being carried out the signal sampling of the T time section by object to be detected, obtains initial light
Compose S2；
Step 3 carries out vacant sample cell using Raman spectrum detector the signal sampling of the T time section, obtains current
Background spectrum S3；
Step 4 calculates the first difference spectrum Sd1 of the reference spectra S1 and the initial spectrum S2, calculates the reference light
The second difference spectrum Sd2 of S1 and current background spectrum S3 is composed, calculates the initial spectrum S2 and the current background spectrum S3
Third difference spectrum Sd3；
Step 5, respectively according to the second difference spectrum Sd2 and the third difference spectrum Sd3 determine reference laser spectral peak with
It is true according to the center difference of the reference laser spectral peak and the object laser spectral peak to be checked respectively by object laser spectral peak to be checked
Fixed first offset △ v1 and the second offset △ v2；
Step 6, the first offset △ v1 and the second offset △ v2, if equal using described first partially
Shifting amount △ v1 are modified the Raman spectrum of testing result；Difference is determined according to the first difference spectrum Sd1 if unequal
It is worth laser spectral peak, third offset △ v3 is then determined according to the center of the difference laser spectral peak, use the third
Offset △ v3 are modified to obtain the 4th offset △ v4 to the offset △ v1, finally using the 4th offset △
V4 is modified the Raman spectrum of testing result.
2. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：The step 5 is also wrapped
Including will use the first offset △ v1 to be directly modified the testing result vf of the standard reference material, and be tied correcting
Fruit obtains the 5th offset △ v5 after being compared with standard Raman spectroscopy, using the 5th offset △ v5 to described first
Offset △ v1 and the second offset △ v2 are modified to obtain final first offset △ v1aWith final second offset
△v2a, use the final first offset △ v1aWith the final second offset △ v2aReplace the first offset △
Step 6 operation is carried out after v1 and the second offset △ v2.
3. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：The Raman spectrum
The optical density parameter of the notch filtering light piece of detector is 6.5~7.5.
4. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：It is additionally included in and carries out institute
It is first placed in dark place before the operation of one~step 6 of the step of stating and first carries out the step 1~preposition school of step 6 progress in advance
It is accurate.
5. a kind of Raman spectrum detector calibration method according to claim 4, it is characterised in that：The preposition calibration
Step 1~step 6 is further included has carried out luminous environment preliminary calibration using standard incandescent lamp offer bias light.
6. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：When the described second offset
When measuring △ v2 as 0 value, result is directly exported without correcting in the step six.
7. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：When the described first offset
When measuring △ v1 as 0 value, directly the Raman spectrum of testing result is carried out using the second offset △ v2 in the step 6
It corrects.
8. a kind of Raman spectrum detector calibration method according to claim 1, it is characterised in that：The canonical reference
Object is polystyrene.
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| CN201810046148.7A CN108169208B (en) | 2018-01-17 | 2018-01-17 | Raman spectrum detector calibration method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977152A (en) * | 2004-06-30 | 2007-06-06 | 凯米映像公司 | Method and apparatus for peak compensation in an optical filter |
| CN102608095A (en) * | 2010-06-25 | 2012-07-25 | 清华大学 | Method for automatically calibrating Raman spectrum detection system by utilizing standard sample |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
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2018
- 2018-01-17 CN CN201810046148.7A patent/CN108169208B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1977152A (en) * | 2004-06-30 | 2007-06-06 | 凯米映像公司 | Method and apparatus for peak compensation in an optical filter |
| CN102608095A (en) * | 2010-06-25 | 2012-07-25 | 清华大学 | Method for automatically calibrating Raman spectrum detection system by utilizing standard sample |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
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