CN108169058A - A kind of method of crude fat content in rapid and accurate determination feed - Google Patents
A kind of method of crude fat content in rapid and accurate determination feed Download PDFInfo
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- CN108169058A CN108169058A CN201711373539.1A CN201711373539A CN108169058A CN 108169058 A CN108169058 A CN 108169058A CN 201711373539 A CN201711373539 A CN 201711373539A CN 108169058 A CN108169058 A CN 108169058A
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- filter paper
- paper packet
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- petroleum ether
- crude fat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N5/00—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid
- G01N5/04—Analysing materials by weighing, e.g. weighing small particles separated from a gas or liquid by removing a component, e.g. by evaporation, and weighing the remainder
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- Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of method of crude fat content in rapid and accurate determination feed, sample is placed on qualitative filter paper formation filter paper packet, filter paper be responsible for a task until it is completed it is dry after first carry 1.5 hours with petroleum ether hot dipping, 2h is extracted again, extraction time just foreshortens to 3.5 hours in this way, reduces half substantially, by dual extraction, crude fat recovery rate is high, assay is accurate, while can not be limited by recycle unit quantity, can be required according to experimental amount, multiple samples are disposably made, increase amount detection.
Description
Technical field
The present invention relates to the measure of crude fat content in feed, and in particular to thick fat in a kind of quick, Accurate Determining feed
The method of fat content.
Background technology
Fat is a major class nutriment, and the important function in animal nutrition is increasingly paid attention to.Fat content
Height is to weigh one of important indicator of nutritive value in feed, therefore the measure of fat becomes the item of feed conventional analysis already
Mesh.There are many method for measuring fat content both at home and abroad, such as soxhlet extraction, infusion method, refractometer method, saponification method, hydrometer method.
At present, the prevailing for the time being in force and relatively broad detection method used is GB/T 6433-2006《The measure of crude fat in feed》,
That is soxhlet extraction, but this method is that extraction times are long in the problem of practical operation, need to extract needs 6~8 hours, nothing
Method comparatively fast obtains experimental result；In addition, this method amount detection is limited by circulating extraction device quantity, it can only detect and follow every time
The comparable sample of ring extraction equipment quantity, has been greatly reduced detection limit, it is impossible to meet actual industrial production demand.
Invention content
The technical problems to be solved by the invention be to provide a kind of minute is short, amount detection is more, detection material it is cheap,
Be easy to get and in the accurate feed of detection data crude fat content assay method.
Technical solution is used by the present invention solves above-mentioned technical problem：Crude fat in a kind of rapid and accurate determination feed
The method of content, its step are as follows：
A, the Feed Sample that quality is M is placed on qualitative filter paper, by qualitative filter paper doubling, doubling firmly wraps sample again for side
It is rolled among qualitative filter paper, obtains filter paper packet；
B, filter paper packet is put into thermostatic drying chamber, the dry constant weight 2h at a temperature of 101~105 DEG C takes out filter paper packet and puts immediately
Enter and room temperature is cooled in drier, filter paper packet quality is referred to as M1；
C, the filter paper packet after above-mentioned steps b is put into port grinding bottle, adds in petroleum ether submergence filter paper packet, port grinding bottle connection returned cold
Solidifying pipe, and port grinding bottle is placed in 43~47 DEG C of water-baths, maintain petroleum ether fluidized state in port grinding bottle, extraction heat 1.5h；Heat
Leaching process can extract 90% or so crude fat within 1.5 hours；
D, the filter paper packet after above-mentioned steps c is put into extraction tube, filter paper packet is made to be completely submerged in petroleum ether, connect condensed water
Flow is stripped 2h in the water bath with thermostatic control for being 43~47 DEG C in temperature；2 hours are carried by extraction tube leaching, it will be remaining a small amount of
Crude fat extract；
E, after extracting, filter paper packet is taken out with long tweezers, makes petroleum ether 5~10min of volatilization in ventilation at room temperature, places into
In baking oven, the dry constant weight 2h at a temperature of 101~105 DEG C, taking-up filter paper packet, which is immediately placed in drier, is cooled to room temperature, claims filter
Paper bag quality is M2, crude fat content is calculated：X=（M1-M2）×100/M.
Compared with prior art, the advantage of the invention is that in a kind of rapid and accurate determination feed crude fat content side
Method, sample are placed on qualitative filter paper formation filter paper packet, filter paper be responsible for a task until it is completed it is dry after first carry 1.5 hours with petroleum ether hot dipping, then extract
2h, such extraction time just foreshorten to 3.5 hours, reduce half substantially, and by dual extraction, crude fat recovery rate is high, contains
Measurement is fixed really, while can not be limited by recycle unit quantity, can be required according to experimental amount, disposably be made multiple samples,
Increase amount detection.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1
The fish meal that 2.0512g is handled well accurately is weighed, is placed on qualitative filter paper, by filter paper doubling, side doubling again, finally by sample
Product are firmly wrapped among filter paper, obtain filter paper packet；Filter paper packet is put into thermostatic drying chamber, at 101~105 DEG C, constant temperature
Dry constant weight 2h, takes out, is immediately placed in drier and is cooled to room temperature（General 15min or so）, claim filter paper packet amount, quality is
2.6624g；Filter paper packet is put into port grinding bottle, adding in petroleum ether makes it submerge filter paper packet, and port grinding bottle connects reflux condensing tube, and
Port grinding bottle is placed in 43~47 DEG C of water-baths, petroleum ether fluidized state is maintained to carry out hot dipping and carries processing 1.5h, it can be by 90% left side
Right crude fat extracts；Filter paper packet is put into extraction tube again, filter paper packet is made to be completely submerged in petroleum ether, connects condensation
Water flow is stripped 2h in water bath with thermostatic control, and water bath with thermostatic control water temperature can make the oil dripped under condensation between 43~47 DEG C
Ether is into beaded（7 times/more than h of 120-150 drops/min or reflux）, 2h is extracted, extracts the petroleum ether filter paper drop in cylinder
Check basic oil stains-less；After extracting, filter paper packet, room temperature are taken out with long tweezers（It is advisable with 12-25 DEG C of room temperature）Under divulging information
Place makes petroleum ether volatilize 5~10min, and the petroleum ether in port grinding bottle and extraction flask can be recycled separately；The petroleum ether that filter paper is wrapped
After volatilization, filter paper packet is placed in baking oven, dry constant weight 2h, is put into drier and is cooled to room temperature at 101~105 DEG C（One
As 15min）, finally claim filter paper packet amount, quality 2.5090g, with formula X=（M1-M2）× 100/M, result of calculation 7.48%,
I.e. the crude fat content of fish meal sample is 7.48%.
Embodiment 2
Substantially the same manner as Example 1, the different fish meal that only 2.0512g is handled well change the DDGSS handled well as 2.0109g
(maize alcohol lees and its soluble matter), filter paper packet amount weigh for the first time, quality 2.5217g；It weighs again, quality is
2.3546g；Result of calculation is 8.31%, i.e., the crude fat content of DDGS samples is 8.31%.
Embodiment 3
Substantially the same manner as Example 1, the different fish meal that only 2.0512g is handled well change the cold water handled well as 2.0307g
Fish meal, filter paper packet amount weigh for the first time, quality 2.9171g；It weighs again, quality 2.6131g；Result of calculation is
14.97%, i.e. the crude fat content of cold water fish forage sample is 14.97%.
Claims (1)
1. a kind of method of crude fat content in rapid and accurate determination feed, it is characterised in that step is as follows：
A, the Feed Sample that quality is M is placed on qualitative filter paper, by qualitative filter paper doubling, doubling firmly wraps sample again for side
It is rolled among qualitative filter paper, obtains filter paper packet；
B, filter paper packet is put into thermostatic drying chamber at a temperature of 101~105 DEG C dry constant weight 2h, takes out filter paper packet and be immediately placed in
Room temperature is cooled in drier, filter paper packet quality is referred to as M1；
C, the filter paper packet after above-mentioned steps b is put into port grinding bottle, adds in petroleum ether submergence filter paper packet, port grinding bottle connection returned cold
Solidifying pipe, and port grinding bottle is placed in 43~47 DEG C of water-baths, petroleum ether fluidized state in port grinding bottle is maintained, hot dipping carries 1.5h；
D, the filter paper packet after above-mentioned steps c is put into extraction tube, filter paper packet is made to be completely submerged in petroleum ether, connect condensed water
Flow is stripped 2h in the water bath with thermostatic control for being 43~47 DEG C in temperature；
E, after extracting, filter paper packet is taken out with long tweezers, makes petroleum ether 5~10min of volatilization in ventilation at room temperature, places into
In baking oven, the dry constant weight 2h at a temperature of 101~105 DEG C, taking-up filter paper packet, which is immediately placed in drier, is cooled to room temperature, claims filter
Paper bag quality is M2, crude fat content is calculated：X=（M1-M2）×100/M.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711373539.1A CN108169058A (en) | 2017-12-19 | 2017-12-19 | A kind of method of crude fat content in rapid and accurate determination feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711373539.1A CN108169058A (en) | 2017-12-19 | 2017-12-19 | A kind of method of crude fat content in rapid and accurate determination feed |
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| CN108169058A true CN108169058A (en) | 2018-06-15 |
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| CN201711373539.1A Pending CN108169058A (en) | 2017-12-19 | 2017-12-19 | A kind of method of crude fat content in rapid and accurate determination feed |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109323952A (en) * | 2018-12-18 | 2019-02-12 | 日照普惠动物营养科技有限公司 | A kind of method of crude fat content in batch detection feedstuff |
| CN114923804A (en) * | 2022-03-09 | 2022-08-19 | 陇南市祥宇油橄榄开发有限责任公司 | Method for determining fat content in olive fruit |
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| CN102590012A (en) * | 2011-12-20 | 2012-07-18 | 苏州大学 | Method for measuring gel rate of silk flosses |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109323952A (en) * | 2018-12-18 | 2019-02-12 | 日照普惠动物营养科技有限公司 | A kind of method of crude fat content in batch detection feedstuff |
| CN114923804A (en) * | 2022-03-09 | 2022-08-19 | 陇南市祥宇油橄榄开发有限责任公司 | Method for determining fat content in olive fruit |
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Application publication date: 20180615 |
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