CN108165659A - A kind of molecule labelling method for improving soybean protein content and its label combination - Google Patents
A kind of molecule labelling method for improving soybean protein content and its label combination Download PDFInfo
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- CN108165659A CN108165659A CN201810202399.XA CN201810202399A CN108165659A CN 108165659 A CN108165659 A CN 108165659A CN 201810202399 A CN201810202399 A CN 201810202399A CN 108165659 A CN108165659 A CN 108165659A
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
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Abstract
The present invention relates to a kind of molecule labelling methods for improving soybean protein content, include the following steps：1）The distribution of soybean RIL groups protein content：The plantation of continuous 2 years is carried out to the advanced lines RIL groups of sliding skin beans and neat Huang 26, counts its protein content；2）The QTL positioning of soybean protein content：QTL positioning is carried out to the protein content of soybean RIL groups using the dense genetic map of SLAF seq technologies structure, obtains 8 relevant QTL sites of soybean protein content.The label combination proposed using the invention carries out breeding material the label of low generation and selects, and helps to assemble the albumen favorable genes in breeding material in the low generation, reduces advanced lines breeding selection workload, reduce Breeding Scale, shortens the breeding time limit.
Description
Technical field
The present invention relates to molecular mark technical field more particularly to a kind of molecules for improving soybean protein content
Labeling method and its label combination.
Background technology
Soybean is important grain and oil and raises dual-purpose crop, has important strategic position in the national economic development.In soybean
Rich in protein, content is more than twice of the cereal crops such as wheat, rice, usually between 40% ~ 50%, in protein
Essential amino acids content is abundant, proportioning is coordinated, and is sufficiently close to the required ratio of Human Physiology, can effectively replace animal protein
Nutritional ingredient.Meanwhile soybean protein has good functional characteristic, can be used in numerous food system, improves product matter
Amount.
At present, the QTL related to soybean protein that SoyBase databases are included amounts to 221.But soybean protein content
It is the quantitative character of controlled by multiple genes, albumen correlation QTL has material-specific, it is impossible to be common to point of specific breeding population
Sub- marker assisted selection breeding.
Invention content
In order to solve the above technical problem, the present invention provides it is a kind of improve soybean protein content molecule labelling method and its
Label combination.The label combination proposed using the invention carries out breeding material the label of low generation and selects, and contributes to low
The albumen favorable genes in breeding material are assembled from generation to generation, reduce advanced lines breeding selection workload, reduce Breeding Scale, are shortened
The breeding time limit.
The present invention is achieved through the following technical solutions, and the present invention provides a kind of molecule mark for improving soybean protein content
Note method, includes the following steps：
1）The distribution of soybean RIL groups protein content：To the advanced lines RIL of sliding skin beans and neat Huang 26（Recombinant inbred lines）Group into
The row plantation of continuous 2 years, counts its protein content；
2）The QTL positioning of soybean protein content：Utilize SLAF-seq technologies（Extensive Genotyping high-throughput strategy technology）Structure
The dense genetic map built carries out QTL positioning to the protein content of soybean RIL groups, obtains 8 soybean protein content correlations
QTL site.
Preferably, neat Huang 26 is by Crop Inst. of shandong Prov. Agriculture science Academy's selection and breeding.
The high protein soybean varieties that Qi Huang26Shi Shandong academy of agricultural sciences crop is bred as, Hua Pidoushi Shandong academy of agricultural sciences make
The excellent soybean germplasm that object is collected, in soybean breeder with being widely used in production.The present invention constructs neat Huang
26 and the RIL groups of sliding skin beans, protein related gene is positioned using SLAF-seq technologies, obtains 8 albumen correlation QTL
Site, phenotype contribution rate 77.14%.These QTL sites will be in the molecular mark based on neat yellow 26 and sliding skin beans
It plays an important role.
The label combination that a kind of molecule labelling method for improving soybean protein content obtains, contains to obtain 8 soybean proteins
Measure relevant QTL site, and 8 relevant QTL sites of soybean protein content be located at respectively No. 1, No. 3, No. 4, No. 5, No. 8,10
Number, No. 15 and No. 16 chromosomes.
Beneficial effects of the present invention are：
1. when carrying out high-protein soybean breeding as parent using neat Huang 26, the label proposed using the invention is combined to educating
Kind material carries out the label selection of low generation, helps to assemble the albumen favorable genes in breeding material in the low generation, reduce
Advanced lines breeding selection workload reduces Breeding Scale, shortens the breeding time limit.
2. when carrying out high-protein soybean breeding as parent to slide skin beans, combined using the label that the invention is proposed
The label that the low generation is carried out to breeding material selects, and helps to assemble the albumen favorable genes in breeding material in the low generation,
Advanced lines breeding selection workload is reduced, reduces Breeding Scale, shortens the breeding time limit.
3. the part marker site physics section that the present invention is obtained is shorter, the fine fixed of protein related gene is realized
Position contributes to the molecular cloning of protein related gene.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the distribution of soybean RIL groups protein content in 2013；
Fig. 2 is the distribution of soybean RIL groups protein content in 2014；
Fig. 3 is that the QTL of soybean protein content is positioned.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings and specific embodiment is further described the application principle of the present invention.
A kind of embodiment 1, molecule labelling method for improving soybean protein content, includes the following steps：
1）The distribution of the protein content of soybean RIL groups：The advanced lines RIL groups of sliding skin beans and neat Huang 26 are carried out continuous 2 years
Plantation, count its protein content, it is found that the protein content of RIL groups is in continuously distributed substantially；As shown in Figs. 1-2.
2）The QTL positioning of soybean protein content：Using the dense genetic map built based on SLAF-seq technologies to big
The protein content of beans RIL groups carries out QTL positioning, using complete Interval mapping（ICIM）8 soybean protein content phases are obtained altogether
The QTL site of pass（Fig. 3）, respectively positioned at No. 1, No. 3, No. 4, No. 5, No. 8, No. 10, No. 15 and No. 16 chromosome（Table 1）, solve altogether
Release 77.14% phenotypic variation.As shown in Figure 3.
The relevant QTL site of 1 soybean protein content of table
Certainly, above description is also not limited to the example above, the technical characteristic of the invention without description can by or use
The prior art realizes that details are not described herein；It is pair that above example and attached drawing, which are merely to illustrate technical scheme of the present invention not being,
The limitation of the present invention, is described in detail the present invention with reference to preferred embodiment, and those of ordinary skill in the art should
Work as understanding, variation that those skilled in the art are made in the essential scope of the present invention, is added or replaced remodeling
Changing without departure from spirit of the invention, should also belong to the claims of the present invention.
Claims (3)
1. a kind of molecule labelling method for improving soybean protein content, it is characterised in that：Include the following steps：
The distribution of soybean RIL groups protein content：The kind of continuous 2 years is carried out to the advanced lines RIL groups of sliding skin beans and neat Huang 26
It plants, counts its protein content；
The QTL positioning of soybean protein content：Using the dense genetic map of SLAF-seq technologies structure to soybean RIL groups
Protein content carries out QTL positioning, obtains 8 relevant QTL sites of soybean protein content.
2. a kind of molecule labelling method for improving soybean protein content according to claim 1, it is characterised in that：Neat Huang 26
Number by Crop Inst. of shandong Prov. Agriculture science Academy's selection and breeding.
3. the label combination that a kind of molecule labelling method for improving soybean protein content according to claim 1 obtains,
It is characterized in that：To obtain 8 relevant QTL sites of soybean protein content, and the relevant QTL site 1 of 8 soybean protein contents
Number, No. 3, No. 4, No. 5, No. 8, No. 10, No. 15 and No. 16 chromosomes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810202399.XA CN108165659A (en) | 2018-03-13 | 2018-03-13 | A kind of molecule labelling method for improving soybean protein content and its label combination |
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| CN201810202399.XA CN108165659A (en) | 2018-03-13 | 2018-03-13 | A kind of molecule labelling method for improving soybean protein content and its label combination |
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| CN108165659A true CN108165659A (en) | 2018-06-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486993A (en) * | 2018-12-06 | 2019-03-19 | 江苏沿海地区农业科学研究所 | A kind of selection of high-protein soybean germplasm |
| CN113322339A (en) * | 2021-05-27 | 2021-08-31 | 东北农业大学 | Molecular marker related to high protein content of soybean and method for identifying soybean with high protein content |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150337397A1 (en) * | 2007-07-06 | 2015-11-26 | Monsanto Technology Llc | Methods and Compositions for Selecting Soybean Plants Resistant to Brown Stem Rot |
| CN106755561A (en) * | 2017-03-30 | 2017-05-31 | 吉林省农业科学院 | A kind of QTL related to soybean root dry weight, SNP marker and application |
-
2018
- 2018-03-13 CN CN201810202399.XA patent/CN108165659A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150337397A1 (en) * | 2007-07-06 | 2015-11-26 | Monsanto Technology Llc | Methods and Compositions for Selecting Soybean Plants Resistant to Brown Stem Rot |
| CN106755561A (en) * | 2017-03-30 | 2017-05-31 | 吉林省农业科学院 | A kind of QTL related to soybean root dry weight, SNP marker and application |
Non-Patent Citations (2)
| Title |
|---|
| YING-HUILI等: "Genome-wide association mapping of QTL underlying seed oil and protein contents of a diverse panel of soybean accessions", 《PLANT SCI.》 * |
| 林延慧等: "大豆蛋白质含量的QTL定位", 《大豆科学》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486993A (en) * | 2018-12-06 | 2019-03-19 | 江苏沿海地区农业科学研究所 | A kind of selection of high-protein soybean germplasm |
| CN113322339A (en) * | 2021-05-27 | 2021-08-31 | 东北农业大学 | Molecular marker related to high protein content of soybean and method for identifying soybean with high protein content |
| CN113322339B (en) * | 2021-05-27 | 2022-02-22 | 东北农业大学 | Molecular marker related to high protein content of soybean and method for identifying soybean with high protein content |
| CN114182045A (en) * | 2021-05-27 | 2022-03-15 | 东北农业大学 | Soybean high-protein-content related molecular marker located on chromosome 14 and method for identifying soybeans with high protein content |
| CN114395640A (en) * | 2021-05-27 | 2022-04-26 | 东北农业大学 | Soybean high-protein-content related molecular marker located on chromosome 6 and method for identifying soybeans with high protein content |
| CN114182045B (en) * | 2021-05-27 | 2023-08-18 | 东北农业大学 | Soybean high-protein content related molecular marker located on chromosome 14 and method for identifying soybean high-protein content |
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