CN108164005A - A kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol - Google Patents
A kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol Download PDFInfo
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- CN108164005A CN108164005A CN201810037097.1A CN201810037097A CN108164005A CN 108164005 A CN108164005 A CN 108164005A CN 201810037097 A CN201810037097 A CN 201810037097A CN 108164005 A CN108164005 A CN 108164005A
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- magnoliae officinalis
- cortex magnoliae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/34—Organic compounds containing oxygen
- C02F2101/345—Phenols
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- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
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- Organic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention belongs to organic pollution processing technology fields, disclose a kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol, the present invention is according to Phenols Catalyzed by Peroxidase in Nonaqueous Media class substance oxidation reaction principle, oxidants hydrogen peroxide is added in using artificial, organic matter NP is removed by Catalyzed Synthesis By Peroxidase, proposes a kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation NP.Cortex Magnoliae Officinalis is transplanted as the medicinal plant of Chinese tradition extensively in Chinese Mountainous Region of Southwest Hubei, resourceful, and blade is easier to obtain；Enzymatic reaction has the characteristics that high catalytic efficiency, reaction condition be mild, controllability, the effect of decomposition NP can be greatly improved by decomposing NP using Cortex Magnoliae Officinalis blade crude enzyme liquid, it also has extraordinary elimination effect for some other phenolic compounds simultaneously, has at low cost, green, safe and pollution-free characteristic.
Description
Technical field
The invention belongs to organic pollution processing technology field more particularly to a kind of utilization Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyls
The method of base phenol.
Background technology
Nonyl phenol (Nonylphenol), abbreviation NP.It is a kind of persistence organic pollutant, belongs in typical phenols and divide
Chaff interferent is secreted, there is toxicity, refractory organics, bioconcentration and estrogenic activity, is the drop of nonyl phenol ethoxylate (NPE)
Solve one of product.NPE is highly important nonionic surface active agent, and the textile industries process such as be widely used in printing and dyeing, clean.
And except textile industry, NPE is also used as surfactant, detergent, is the environmental hormone that the whole world is generally acknowledged, such surface
Activating agent is once entered in environment, ethyoxyl will be gone to react rapidly, resolves into the stronger environmental hormone-NP of toxicity.
In recent years, to the demand cumulative year after year of NPE, incident is the discharge of a large amount of NP pollutants in China.NP has that " sperm kills
The title of hand " can be absorbed by the body by skin so as to the endocrine, reproduction and immune system of interfering human and animal, can
Promote Cells Proliferation of Human Breast Cancer, it seriously can be carcinogenic.Research shows that cell survival rate is notable in high dose NP and their mixture
Declining, cell is more sensitive to NP in breeding, even if concentration is very low in environment, also great harmfulness.In recent years, NP existed
In water body concentrations have year by year it is increased become Potential.Therefore, how to handle NP in water environment becomes urgent need to solve the problem.It is thick
Piao, the purple Piao of alias, purple oil Piao, warm Piao etc., is Magnoliaceae, magnolia, is planted extensively as the medicinal plant of Chinese tradition
It plants in Chinese Mountainous Region of Southwest Hubei.Traditional Cortex Magnoliae Officinalis planting industry mainly extracts pharmaceutically acceptable ingredient magnolol therein using Magnolia bark
And honokiol.Magnolol and honokiol are the principal active components of Cortex Magnoliae Officinalis, are primarily present in bark, have it is anti-oxidant,
The pharmacological actions such as anti-inflammatory, antitumor and antibacterial.Under normal circumstances, Cortex Magnoliae Officinalis, which is grown 15 years or more, can just strip dry hide and branch skin, add
Work is into available Chinese medicine, and during long term growth, a large amount of Cortex Magnoliae Officinalis blades are not utilized effectively, and inventor passes through survey
Fixed to confirm, peroxidase activity occupy forefront in Magnoliacea plant in Leaf of Magnolia officinalis.At present, the processing method of NP generally with
Physical, chemical method, bioanalysis be representative three big treatment technologies, including burning method, active carbon adsorption, chemical method,
Solvent extraction, microbial degradation method etc..It is a kind of relatively effective processing method using powder electroless plating, but drawback exists
It is not thorough in removing, it can only be as the emergency method of sudden water pollution.In addition, Ti/SnO2—Sb2O5Electrode Electrocatalysis Degradation
Method power consumption is big, complex procedures, economic benefit are low.O3 catalytic oxidation is a kind of place for effectively removing Organic Pollutants in Wastewater
Reason method has synthesized a series of oxidation manganese material of different-shapes, phase, valence state and composition, applied to catalysis ozone at present
Oxidative degradation phenolic comp ' ds pollution, but this method often brings secondary pollution, because of inherently a kind of noxious material of ozone,
It is and expensive, it is difficult to promote the use of on a large scale.In recent years, the microbial method in bioanalysis is with its density height, processing equipment
The features such as simple, is used widely, although this method safety, practical Sewage Environment is quite severe, and microorganism is to existence
Condition has very big requirement, once in rugged environment, survival ability is necessarily restricted, and utilizes microbial degradation
Phenols wastewater then needs large-scale culture microorganism and time-consuming, space is big, while phenol wastewater has murder by poisoning to microorganism and makees
With.
In conclusion problem of the existing technology is：The processing method of NP is not thorough in the presence of removing at present, power consumption
Greatly, complex procedures, economic benefit are low, expensive, it is difficult to which large-scale promotion uses；It is then needed using microbial degradation phenols wastewater
It wants large-scale culture microorganism and time-consuming, space is big, while there are toxic actions to microorganism for phenol wastewater；Microbe survival
Ability is necessarily restricted, it is difficult to realize the efficient process to a large amount of phenols wastewaters.And the biological Enzymology method for passing through plant source
Phenol wastewater is removed, then it is possible to prevente effectively from these above-mentioned drawbacks.
Invention content
In view of the problems of the existing technology, Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized the present invention provides a kind of
Method.
The invention is realized in this way a kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol, described using thick
The method of plain blade crude enzyme liquid degradation nonyl phenol includes：
Step 1 weighs ripe Cortex Magnoliae Officinalis leaf, according to 1：5 solid-liquid ratios are added in containing 2% polyvinylpyrrolidone and 2mmol/L
The citrate phosphate buffer of ethylenediamine tetra-acetic acid, 4 DEG C of homogenate, 4 layers of filtered through gauze, filtrate is at 4 DEG C, 12000g centrifugations
20min abandons precipitation, retains supernatant；
Step 2 adds in ammonium sulfate in supernatant, reaches 20% saturation degree, precipitates overnight, and centrifugation discards precipitation, takes
Supernatant continuously adds ammonium sulfate, takes the precipitation of 60%~90% saturation degree part, is thick enzyme after being dissolved with appropriate pure water
Liquid；
Step 3 pipettes buffer solution, and control temperature of reaction system is 10~50 DEG C, and pH value is 3~11, adds in appropriate thick
Enzyme solution makes a concentration of 10~20 μm of ol/L of NP in reaction system, adds in the H that molar concentration is NP10~100 times2O2Start reaction,
Reaction time is 180min；Start 1min, 3min, 5min, 15min, 45min, 90min, 120min, 180min from upper in reaction
It states and 1ml is sampled in reaction system, add in 2mL methanol and terminate reaction, realize degradation of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP；It is anti-terminating
It answers and appropriate alum is added in liquid, shake up, 5000g centrifuges 10min after overnight precipitation；Supernatant after centrifugation crosses 0.22 μm of filter membrane
Afterwards, it is detected using liquid chromatogram-mass spectrometer, mobile phase is 80% methanol/water, and Mass Spectrometer Method quasi-molecular ion peak corresponds to
Relative molecular mass be 219.18.
Further, to there is the lemon of polyvinylpyrrolidone, ethylenediamine tetra-acetic acid in the step 1 Cortex Magnoliae Officinalis leaf extract
Acid-phosphate buffer.
Further, the step 1 buffer solution for acetic acid-sodium acetate buffer, borax buffer solution, citric acid-
Any one of phosphate buffer.
Further, the pH value is 7.
Further, the NP initial concentrations are 10~20 μm of ol/L.
Further, the temperature is room temperature.
Further, the reaction time is 180min.
Further, the enzyme addition is 10%.
The present invention adds in oxidant peroxidating according to Phenols Catalyzed by Peroxidase in Nonaqueous Media class substance oxidation reaction principle using artificial
Hydrogen removes organic matter NP by Catalyzed Synthesis By Peroxidase, proposes a kind of method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation NP.Cortex Magnoliae Officinalis is made
For Chinese tradition medicinal plant and transplanted extensively in Chinese Mountainous Region of Southwest Hubei, resourceful, blade is easier to obtain；Enzyme
Promoting reaction has the characteristics that high catalytic efficiency, reaction condition be mild, controllability, can be with using Cortex Magnoliae Officinalis blade crude enzyme liquid decomposition NP
The effect for decomposing NP is greatly improved, while it also has extraordinary elimination effect for some other phenolic compounds, has
The characteristics such as at low cost, green, safe and pollution-free.This method is using plant peroxidases catalytic oxidation degradation NP, drop
Solve efficient, and used raw material Cortex Magnoliae Officinalis blade easily obtains, have the advantages that efficiently, green, contaminate without two pollutions；And it can fill
Divide using the Cortex Magnoliae Officinalis blade largely abandoned generated in Cortex Magnoliae Officinalis production link, provided for vast Cortex Magnoliae Officinalis farmer new
Income source improves the overall economic efficiency of Cortex Magnoliae Officinalis plantation.
Description of the drawings
Fig. 1 is the method flow diagram provided in an embodiment of the present invention using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol.
Fig. 2 is the removing situation schematic diagram of NP under different enzyme concentrations provided in an embodiment of the present invention.
Fig. 3 is NP NP surplus ratios schematic diagrames in 3 hours under condition of different temperatures provided in an embodiment of the present invention.
Fig. 4 is NP surplus ratio schematic diagrames in 3 hours under condition of different pH provided in an embodiment of the present invention.
Fig. 5 is that difference NP provided in an embodiment of the present invention and NP under the conditions of hydrogen peroxide molar concentration rate was remained in 3 hours
Remaining rate schematic diagram.
Fig. 6 is NP surplus ratio schematic diagrames in 3 hours under the conditions of various concentration FA provided in an embodiment of the present invention.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in Figure 1, it is provided in an embodiment of the present invention using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol method include with
Lower step：
S101：Ripe Cortex Magnoliae Officinalis leaf is weighed, according to 1：5 solid-liquid ratios add in containing 2% polyvinylpyrrolidone (PVP-K30) and
The citrate phosphate buffer (50mmol/L, pH 7.0) of 2mmol/L ethylenediamine tetra-acetic acids (EDTA), 4 DEG C of homogenate, 4 layers of gauze
Filtering, at 4 DEG C, 12000g centrifugation 20min abandon precipitation, retain supernatant, as Cortex Magnoliae Officinalis blade zyme extract filtrate；
S102：Ammonium sulfate is added in above-mentioned supernatant, reaches 20% saturation degree, precipitates overnight, centrifugation (at 4 DEG C,
12000g centrifuges 15min), precipitation is discarded, takes supernatant, continuously adds ammonium sulfate, takes the heavy of 60%~90% saturation degree part
It forms sediment, is Cortex Magnoliae Officinalis blade crude enzyme liquid after being dissolved with appropriate pure water.It is spare to measure enzymatic activity, the assay method of enzyme activity：Using more wound
Wooden phenol method.It adds in 0.1ml enzyme solutions and starts reaction, the variation of OD values is recorded at wavelength 470nm, 30 DEG C.1 enzyme unit (U) definition
For the enzyme amount needed for OD470 variations 0.1 per minute under determination condition；
S103：Buffer solution is pipetted, control temperature of reaction system is 10~50 DEG C, and pH value is 3~11, adds in appropriate thick enzyme
Liquid makes a concentration of 10~20 μm of ol/L of NP in reaction system, adds in the H that molar concentration is 10~100 times of NP2O2Start reaction,
Reaction time is 180min.Start 1min, 3min, 5min, 15min, 45min, 90min, 120min, 180min from upper in reaction
It states and 1ml is sampled in reaction system, add in 2mL methanol and terminate reaction, that is, realize degradation of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP.It is terminating
Appropriate alum is added in reaction solution, is shaken up, 5000g centrifuges 10min after overnight precipitation.Supernatant after centrifugation crosses 0.22 μm of filter membrane
Afterwards, it is detected using liquid chromatogram-mass spectrometer, mobile phase is 80% methanol/water, and Mass Spectrometer Method quasi-molecular ion peak corresponds to
Relative molecular mass be 219.18.
The application principle of the present invention is further described with reference to specific embodiment.
The influence that 1 Cortex Magnoliae Officinalis blade crude enzyme liquid difference enzyme concentration of embodiment degrades to NP.
Each reaction system is respectively placed in the reagent bottle of 6 numbers, in total 6 reagent bottles, is added in each reagent bottle
Enter pH value be 7 phosphoric acid-citrate buffer solution, add a concentration of 22mg/L 40 μ L of NP solution, be then respectively adding enzyme solution
100U, 200U, 500U, 800U, 1200U, the common 20mL of reaction system take 1mL samples to add in 2mL methanol as blank control, Xiang Qi
It is middle to add in clean magnetic stir bar.Reagent bottle is respectively placed in thermostat water bath under room temperature and is stirred, according to NP moles it is dense
Degree is than being 20：1 add in hydrogenperoxide steam generator reaction 180min, in reaction 1min, 3min, 5min, 15min, 45min, 90min,
120min, 180min take 1mL reaction mixtures, add in 2mL methanol and terminate reaction, that is, realize drop of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP
Solution.Appropriate alum is added in reaction solution terminating, is shaken up, 5000g centrifuges 10min after overnight precipitation.Supernatant sample after centrifugation
Product detected after taking out 0.22 μm of film in liquid phase-mass spectrometer, ultraviolet detection wavelength be 220nm, Mass Spectrometer Method peak pair
The relative molecular mass answered is about 219.18, and mobile phase is 80% methanol/water.Obtained concrete outcome is as shown in Fig. 2, from figure
As can be seen that when reaction system enzyme concentration is 60U/ml, NP degradation rate highests are 100%, cannot be complete less than this enzyme concentration
Clear all.
Influence of 2 condition of different temperatures of embodiment to Cortex Magnoliae Officinalis blade crude enzyme liquid degradation NP.
6 reagent bottles are taken, phosphoric acid-appropriate citrate buffer solution that pH value is 7 is added in each reagent bottle, adds NP
Solution, make final concentration of 22mg/L then add in enzyme solution, make total volume for 20mL (insufficient section, using pH value as 7 phosphoric acid-
Citrate buffer solution supplements), mixing takes 1mL mixed liquors, adds in 2mL methanol as blank control, magnetic force is added in mixed liquor
Stirrer.Reagent bottle is respectively placed in water temperature as 10 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, 40 DEG C, 50 DEG C of temperature constant magnetic stirring water-bath
It is stirred in pot, is 20 according to NP molar concentration rates：1 add in hydrogenperoxide steam generator, react 180min, in reaction 1min, 3min,
5min, 15min, 45min, 90min, 120min, 180min take 1mL reaction mixtures respectively from reaction mixture, add in 2mL
Methanol terminates reaction, that is, realizes degradation of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP.Obtained concrete outcome is as shown in figure 3, can from Fig. 3
To find out, when temperature is in 10 DEG C~50 DEG C of ranges, reacted by 3 hours, NP is completely removed, this shows that the clearance technique exists
In the annual overwhelming majority time, it can achieve the effect that fully erased NP at room temperature, there is good Acclimation temperature range.
Influences of the embodiment 3pH to Cortex Magnoliae Officinalis blade crude enzyme liquid degradation NP.
Each reaction system is respectively placed in the reagent bottles of 9 numbers, in total 9 reagent bottles, with acetic acid-sodium acetate,
Phosphoric acid-citric acid, borate buffer solution control pH value of reaction system for 3,4,5,6,7,8,9,10,11, add a concentration of
The NP solution of 22mg/L and then enzyme solution being added in, common 20mL takes 1mL reaction mixtures addition 2mL methanol as blank control, to
Wherein add in clean magnetic stir bar.Reagent bottle is respectively placed in magnetic stirring apparatus and is reacted at normal temperatures, according to
NP molar concentration rates are 20：1 add in hydrogen peroxide start reaction, in reaction 1min, 3min, 5min, 15min, 45min,
90min, 120min, 180min take 1mL reaction mixtures, add in 2mL methanol and terminate reaction, that is, realize Cortex Magnoliae Officinalis blade crude enzyme liquid pair
The degradation of NP.Obtained result is specific as shown in figure 4, it can be seen from the figure that almost can be by for 3~11, NP in pH value
Fully erased, wherein pH more low reactions are rapider, it can be seen that, Cortex Magnoliae Officinalis peroxidase has very wide pH threshold values, at sewage
Reason downstream is easy to apply.
4 difference NP of embodiment and NP surplus ratios in 3 hours under the conditions of hydrogen peroxide molar concentration rate.
Each reaction system is respectively placed in the reagent bottle of 5 numbers, in total 5 reagent bottles, is added in each reagent bottle
Enter pH value be 7 phosphoric acid-citrate buffer solution, add a concentration of 22mg/L NP solution then add in enzyme solution, common 20mL,
1mL reaction mixtures is taken to add in 2mL methanol as blank control, add in clean magnetic stir bar thereto.It rubs according to NP
Your concentration ratio is 2：1、5：1、10：1、20：1、30：1 adds in hydrogenperoxide steam generator, reacts 180min under room temperature, in reaction 1min,
3min, 5min, 15min, 45min, 90min, 120min, 180min take reaction mixture 1mL, add in 2mL methanol and terminate reaction,
Realize degradation of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP.Obtained concrete outcome as shown in figure 5, it can be seen from the figure that when NP with
Hydrogen peroxide molar concentration rate is 1：When 20, NP degradation rates it is most fast and can basically reach it is fully erased, although other concentration ratios
Degradation rate is slightly slow, but as a child can also basically reach 3 fully erased.
NP surplus ratios in 3 hours under the conditions of 5 various concentration humic acid (FA) of embodiment.
Each reaction system is respectively placed in the reagent bottle of 5 numbers, in total 5 reagent bottles, is added in each reagent bottle
Enter phosphoric acid-citrate buffer solution that pH value is 7, the NP solution for adding a concentration of 22mg/L, then add in enzyme solution and FA is molten
Liquid, FA concentration is respectively 0mg/L, 2mg/L, 5mg/L, 10mg/L, 50mg/L in reaction system, and common 20mL takes 1mL reaction mixing
Liquid adds in 2mL methanol as blank control, adds in clean magnetic stir bar thereto.It is 20 according to NP molar concentration rates:1
Add in hydrogenperoxide steam generator, react 180min under room temperature, in reaction 1min, 3min, 5min, 15min, 45min, 90min,
120min, 180min take 1mL reaction mixtures, add in 2mL methanol and terminate reaction, that is, realize drop of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP
Solution.Obtained concrete outcome is as shown in fig. 6, it can be seen from the figure that the FA solution of various concentration has the rate for removing NP
It influences, reaction rate is slack-off, but remains to after 180min to reach basic removing, illustrates that Cortex Magnoliae Officinalis peroxidase is more stable, clearly
It is not very big that division result is influenced by the organic matter FA of 50mg/L.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (8)
- A kind of 1. method using Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol, which is characterized in that described to utilize the thick enzyme of Cortex Magnoliae Officinalis blade The method of liquid degradation nonyl phenol includes：Step 1 weighs ripe Cortex Magnoliae Officinalis leaf, according to 1：5 solid-liquid ratios are added in containing 2% polyvinylpyrrolidone and 2mmol/L second two The citrate phosphate buffer of amine tetraacethyl, 4 DEG C of homogenate, 4 layers of filtered through gauze, filtrate is at 4 DEG C, 12000g centrifugation 20min, Precipitation is abandoned, retains supernatant；Step 2 adds in ammonium sulfate in supernatant, reaches 20% saturation degree, precipitates overnight, and centrifugation discards precipitation, takes supernatant Liquid continuously adds ammonium sulfate, takes the precipitation of 60%~90% saturation degree part, is crude enzyme liquid after being dissolved with appropriate pure water；Step 3 pipettes buffer solution, and control temperature of reaction system is 10~50 DEG C, and pH value is 3~11, adds in appropriate thick enzyme Liquid makes a concentration of 10~20 μm of ol/L of NP in reaction system, adds in the H that molar concentration is NP10~100 times2O2Start reaction, instead It is 180min between seasonable；Start 1min, 3min, 5min, 15min, 45min, 90min, 120min, 180min from above-mentioned in reaction 1ml is sampled in reaction system, 2mL methanol is added in and terminates reaction, realizes degradation of the Cortex Magnoliae Officinalis blade crude enzyme liquid to NP；It is reacted terminating Appropriate alum is added in liquid, is shaken up, 5000g centrifuges 10min after overnight precipitation；After supernatant after centrifugation crosses 0.22 μm of filter membrane, It is detected using liquid chromatography mass combined instrument, mobile phase is 80% methanol/water, and Mass Spectrometer Method quasi-molecular ion peak is corresponding opposite Molecular mass is 219.18.
- 2. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the step It is citric acid-the phosphate buffer for having polyvinylpyrrolidone, ethylenediamine tetra-acetic acid in one Cortex Magnoliae Officinalis leaf extract.
- 3. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the step One buffer solution is any one of acetic acid-sodium acetate buffer, borax buffer solution, citric acid-phosphate buffer.
- 4. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the pH value It is 7.
- 5. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that at the beginning of the NP Begin a concentration of 10~20 μm of ol/L.
- 6. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the temperature For room temperature.
- 7. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the reaction Time is 180min.
- 8. the method for Cortex Magnoliae Officinalis blade crude enzyme liquid degradation nonyl phenol is utilized as described in claim 1, which is characterized in that the enzyme adds Enter amount for 60U/ml.
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Cited By (1)
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| CN109234353A (en) * | 2018-09-14 | 2019-01-18 | 暨南大学 | Microalgae is effectively degraded the experimental method of nonyl phenol |
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| CN109234353A (en) * | 2018-09-14 | 2019-01-18 | 暨南大学 | Microalgae is effectively degraded the experimental method of nonyl phenol |
| CN109234353B (en) * | 2018-09-14 | 2021-10-29 | 暨南大学 | Experimental method for effectively degrading nonyl phenol by microalgae |
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Application publication date: 20180615 |