CN108159505A - A kind of method for preparing overriding resistance bacterium coating on medical catheter surface - Google Patents
A kind of method for preparing overriding resistance bacterium coating on medical catheter surface Download PDFInfo
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- CN108159505A CN108159505A CN201711430163.3A CN201711430163A CN108159505A CN 108159505 A CN108159505 A CN 108159505A CN 201711430163 A CN201711430163 A CN 201711430163A CN 108159505 A CN108159505 A CN 108159505A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/08—Materials for coatings
- A61L29/085—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/14—Materials characterised by their function or physical properties, e.g. lubricating compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/02—Methods for coating medical devices
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The present invention relates to field of medical materials more particularly to a kind of methods for preparing overriding resistance bacterium coating on medical catheter surface.A kind of method for preparing overriding resistance bacterium coating on medical catheter surface, includes the following steps：Medical catheter surface is pre-processed；Initiator is fixed on the surface of medical catheter after the pre-treatment；In the surface grafting of medical catheter to carry the organic molecule of positive charge or polymer with positive charge is monomer, using methacrylate polyethylene glycol as crosslinking agent, cause the chemical graft method of supplement reduction atom transferred free radical using surface, finally prepare polycation layer antimicrobial coating.The coating of the present invention, which has, is grafted uniform, excellent anti-infection property energy, reaches more than 99% to the anti-infective rate of drug-resistant bacteria.
Description
Technical field
A kind of prepare the present invention relates to field of medical materials more particularly on medical catheter surface overriding resistance bacterium coating
Method.
Background technology
Medical catheter is that clinical common polymeric medical instrument is required for contacting with tissue, however in this process
In, bacterium is easily invaded by all means, forms biomembrane, and then cause infection, incidence and mortality is caused to carry significantly
It is high.Patient's average stay extends, and the pain and medical treatment cost born also rise therewith.According to statistics, make in China every 5,000,000
With in the patient of medical catheter, having up to 5% people by the bacterium infection from medical catheter in itself.In this overall background
Under, the medical antibacterial medical catheter with antibacterial functions comes into being.
At present, with the abuse of antibiotic, the type of drug-resistant bacteria also gradually increases, and so as to increase treatment difficulty, passes
The method of system antibiotic medical catheter mainly assigns its anti-infection property energy using in material surface formation functional coating, including logical
It crosses the physical methods such as plasma surface modification or spraying and one layer is formed in medical catheter outer surface containing silver coating, assign its anti-sense
Metachromia；Also there is the method by coating to form one layer of anti-infective organic reagent or antibiotic coating inside and outside medical catheter, assign
Its anti-infection property energy.However these methods are combined force difference there are coating with medical catheter surface, the anti-infectives to come off are to body
Body tissue causes serious injury, it is often more important that, these catheter surface antimicrobial coatings can not effectively kill drug-resistant bacteria.
Invention content
It is resistance to it is an object of the invention to which the antimicrobial coating on medical catheter surface in the prior art is overcome not kill effectively
The defects of medicine bacterium, provides a kind of method for preparing overriding resistance bacterium coating on medical catheter surface.
A kind of method for preparing overriding resistance bacterium coating on medical catheter surface, includes the following steps：
(1) medical catheter surface is pre-processed, this step pretreatment purpose is that medical catheter surface is modified instead
Should, its surface is made to have amino or hydroxyl, obtains amination or hydroxylated medical catheter；
(2) initiator is fixed on the surface of amination or hydroxylating medical catheter, obtains surface and led with the medical of initiator
Pipe；
(3) there is the surface grafting of the medical catheter of initiator to carry the organic molecule of positive charge or carry on surface
The polymer of positive charge is monomer, using methacrylate polyethylene glycol as crosslinking agent, causes-supplement reduction atom using surface
The chemical graft method of transferred free radical finally prepares polycation layer antimicrobial coating.
Preferentially, the medical catheter is high molecular material class medical catheter or silicates medical catheter.
Preferentially, the high molecular material class medical catheter be polyolefins conduit, polyvinyl chloride conduit, silica gel catheter,
Polystyrene.
Preferentially, the silicates medical catheter is grass tube or quartz conduit.
Preferentially, medical catheter surface is pre-processed in step (1), included the following steps：Medical catheter is soaked
Bubble is impregnated, is washed, is dry in the Tris buffer solution containing dopamine, and the temperature of Tris buffer solution is 37 DEG C,
The concentration of Tris buffer solution is 4mg/ml, and the pH value of Tris buffer solution is 8.4.
Preferentially, initiator is fixed on the surface of the medical catheter in step (2) after the pre-treatment, is included the following steps：
Pretreated medical catheter is put into ethyl acetate solvent, then adds in 4-dimethylaminopyridine, triethylamine, bromo isobutyl
Acylbromide, washing, dry after reacting for 24 hours at room temperature, the 4-dimethylaminopyridine concentration is 0.05g/ml, triethylamine concentration
The concentration for being 0.08g/ml bromo isobutyl acylbromides is 0.2g/ml.
Preferentially, the polymer with positive charge is (3- acrylamide propyls) trimethyl ammonium chloride, dimethyl two
Allyl ammonium chloride, methacrylic acid Quaternary Polyethyleneimine, methacrylate epsilon-polylysine or methacrylate
Poly- methylene hydrochloric acid, the concentration of the polymer with positive charge is 1.45mol/L.
Preferentially, the molecular weight of the polymer with positive charge is 800-4000.
Preferentially, the molecular weight of the methacrylate polyethylene glycol is 700-4000.
Preferentially, in the chemical graft method of step (3), include the following steps：With copper bromide/copper/N, N, N', N, '
N "-five methyl diethylentriamine is catalytic ligand system, using the mixed solution of methanol/water as reaction dissolvent, step (2) processing
Rear medical catheter is several, monomer, crosslinking agent react for 24 hours with catalytic ligand system in reaction dissolvent at 50 DEG C, be washed out,
It is dry.
Beneficial effects of the present invention：(1) present invention is a kind of solution poly-dopamine reaction method, and anti-infective is fixed
On medical catheter, using surface cause-supplement reduction atom transferred free radical method in medical catheter surface grafting band just
The cross-linked structure coating of electric polymer, which, which has, is grafted uniform, excellent anti-infection property energy, to the anti-infective of drug-resistant bacteria
Rate reaches more than 99%；(2) present invention can effectively prevent methicillin-resistant staphylococcus aureus and vancomycin enterococcus
The formation of biomembrane, effectively kills drug-resistant bacteria, can solve traditional antibiotic medical catheter face coat using heavy metal ion
The toxic side effects of generation；(3) present invention can solve coating exists in the prior art is combined force difference with medical catheter surface, is applied
The problem of layer is easy to fall off, and the anti-infectives to come off cause serious injury to bodily tissue.
Description of the drawings
Fig. 1 is medical catheter used in the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Fig. 2 is medical catheter used in the present invention to vancomycin enterococcus shows fluorescent microscopy images；
Fig. 3 is 1 gained coating of the embodiment of the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Fig. 4 is 1 gained coating of the embodiment of the present invention to vancomycin enterococcus shows fluorescent microscopy images；
Fig. 5 is 2 gained coating of the embodiment of the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Fig. 6 is 2 gained coating of the embodiment of the present invention to vancomycin enterococcus shows fluorescent microscopy images；
Fig. 7 is 3 gained coating of the embodiment of the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Fig. 8 is 3 gained coating of the embodiment of the present invention to vancomycin enterococcus shows fluorescent microscopy images；
Fig. 9 is 4 gained coating of the embodiment of the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Figure 10 is 4 gained coating of the embodiment of the present invention to vancomycin enterococcus shows fluorescent microscopy images；
Figure 11 is 5 gained coating of the embodiment of the present invention to methicillin-resistant staphylococcus aureus shows fluorescent microscopy images；
Figure 12 is 5 gained coating of the embodiment of the present invention to vancomycin enterococcus shows fluorescent microscopy images.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated.
Embodiment 1
One kind prepares overriding resistance bacterium coating on medical catheter surface, includes the following steps：
1. by the several Tris buffer solution being immersed in containing dopamine of polyolefins medical catheter, at 37 DEG C
For 24 hours, then distilled water cleans for reaction, vacuum drying.A concentration of 4mg/ml of Tris buffer solution, Tris buffer solution
PH=8.4 obtains surface amination modified polyolefin hydro carbons medical catheter.
2. surface amination modified polyolefin hydro carbons medical catheter is several is put into ethyl acetate solvent, ethyl acetate volume
It is 20ml, then adds in 0.05g/ml 4-dimethylaminopyridine, 0.08g/ml triethylamines, 0.2g/ml bromo isobutyl acylbromides,
It after reacting for 24 hours at room temperature, is dried in vacuo after ethyl alcohol cleaning, obtaining surface has the polyolefins medical catheter of initiator.
3. surface has the polyolefins medical catheter of initiator several, with (3- acrylamide propyls) trimethyl ammonium chloride
For monomer, the quality of (3- acrylamide propyls) trimethyl ammonium chloride is 4g, methacrylate polyethylene glycol (molecular weight 700)
For crosslinking agent, the quality of methacrylate polyethylene glycol is 1.013g, using copper bromide/copper/N, N, N', N, ' N "-pentamethyl
Diethylenetriamines is catalytic ligand system, and the quality of copper bromide is 29.1mg, and the quality of copper is 30mg, N, N, N', N, ' N "-
The quality of five methyl diethylentriamine is 46mg, and 50 DEG C of reaction temperature, reaction dissolvent is methanol/water mixed solvent, and mixing is molten
The volume of agent is 10ml, and the volume ratio of methanol/water is 1/1, and the reaction time, ethyl alcohol cleaning was dried in vacuo for 24 hours.
Fig. 1 and Fig. 2 is that the medical catheter used in the present invention cultivates methicillin-resistant staphylococcus aureus (bacterium respectively respectively
Liquid a concentration of 106CFU/ml) with vancomycin enterococcus (106CFU/ml) shows fluorescent microscopy images after 24h.Fig. 3 and Fig. 4 points
It is not in 1 gained coating surface culture methicillin-resistant staphylococcus aureus of the embodiment of the present invention and vancomycin enterococcus
(bacterial concentration 106CFU/ml) the shows fluorescent microscopy images after 24 hours.Compared with Fig. 1, Fig. 2, the coating surface of Fig. 3 and Fig. 4
Bacterial number is reduced, and shows that 1 gained coating of the embodiment of the present invention has certain anti-microbial property.
Embodiment 2
One kind prepares overriding resistance bacterium coating on medical catheter surface, includes the following steps：
1. by the several Tris buffer solution being immersed in containing dopamine of silica type medical catheter.It is anti-at 37 DEG C
Should for 24 hours, then distilled water cleans, vacuum drying.A concentration of 4mg/ml of Tris buffer solution, Tris buffer pH value of solution
=8.4, obtain surface amination modified silica-gel class medical catheter.
It is put into ethyl acetate solvent 2. surface amination modified silica-gel class medical catheter is several, ethyl acetate volume is
Then 20ml adds in 0.05g/ml 4-dimethylaminopyridine, 0.08g/ml triethylamines, 0.2g/ml bromo isobutyl acylbromides, in room
After the lower reaction for 24 hours of temperature, it is dried in vacuo after ethyl alcohol cleaning, obtaining surface has the poly- silica type medical catheter of initiator.
3. surface has the poly- silica type medical catheter of initiator several, using dimethyl diallyl ammonium chloride as monomer,
The quality of dimethyl diallyl ammonium chloride is 2g, with methacrylate polyethylene glycol (molecular weight 1000) for crosslinking agent, first
The quality of base acrylate polyethylene glycol is 1.2g, using copper bromide/copper/N, N, N', N, ' N "-five methyl diethylentriamine
For catalytic ligand system, the quality of copper bromide is 29.1mg, and the quality of copper is 30mg, N, N, N', N, ' the sub- second of N "-pentamethyl two
The quality of base triamine is 46mg, and 50 DEG C of reaction temperature, reaction dissolvent is methanol/water mixed solvent, and the volume of mixed solvent is
10ml, the volume ratio of methanol/water is 1/1, and the reaction time, ethyl alcohol cleaning was dried in vacuo for 24 hours.
Fig. 5 and Fig. 6 is in 2 gained coating surface culture methicillin-resistant staphylococcus aureus of the embodiment of the present invention respectively
With vancomycin enterococcus (bacterial concentration 106CFU/ml) shows fluorescent microscopy images after 24h.Compared with Fig. 1, Fig. 2, Fig. 5
It is reduced with the coating surface bacterial number of Fig. 6, shows that 1 gained coating of the embodiment of the present invention has certain anti-microbial property.
Embodiment 3
One kind prepares overriding resistance bacterium coating on medical catheter surface, includes the following steps：
1. by the several Tris buffer solution being immersed in containing dopamine of polyvinyl chloride medical conduit, at 37 DEG C
For 24 hours, then distilled water cleans for lower reaction, vacuum drying.A concentration of 4mg/ml of Tris buffer solution, Tris buffer are molten
Liquid pH=8.4 obtains surface amination modified silica-gel class medical catheter.
It is put into ethyl acetate solvent 2. surface amination modified silica-gel class medical catheter is several, ethyl acetate volume is
Then 20ml adds in 0.05g/ml 4-dimethylaminopyridine, 0.08g/ml triethylamines, 0.2g/ml bromo isobutyl acylbromides, in room
After the lower reaction for 24 hours of temperature, it is dried in vacuo after ethyl alcohol cleaning, obtaining surface has the poly- silica type medical catheter of initiator.
3. surface have initiator poly- silica type medical catheter it is several, using methacrylic acid Quaternary Polyethyleneimine as
Monomer (molecular weight 800), the quality of methacrylic acid Quaternary Polyethyleneimine is 9g, with methacrylate polyethylene glycol
(molecular weight 4000) is crosslinking agent, and the quality of methacrylate polyethylene glycol is 4.8g, using copper bromide/copper/N, N, N',
N, ' N "-five methyl diethylentriamine is catalytic ligand system, the quality of copper bromide is 29.1mg, and the quality of copper is 30mg, N,
N, N', N, ' the quality of N "-five methyl diethylentriamine is 46mg, 50 DEG C of reaction temperature, reaction dissolvent is methanol/water mixing
Solvent, the volume of mixed solvent is 10ml, and the volume ratio of methanol/water is 1/1, and the reaction time, ethyl alcohol cleaning was dried in vacuo for 24 hours.
Fig. 7 and Fig. 8 is in 3 gained coating surface culture methicillin-resistant staphylococcus aureus of the embodiment of the present invention respectively
With vancomycin enterococcus (bacterial concentration 106CFU/ml) shows fluorescent microscopy images after 24h.Compared with Fig. 1, Fig. 2, Fig. 7
It is reduced with the coating surface bacterial number of Fig. 8, shows that 1 gained coating of the embodiment of the present invention has certain anti-microbial property.
Embodiment 4
One kind prepares overriding resistance bacterium coating on medical catheter surface, includes the following steps：
1. by the several Tris buffer solution being immersed in containing dopamine of polystyrene type medical catheter, at 37 DEG C
For 24 hours, then distilled water cleans for lower reaction, vacuum drying.A concentration of 4mg/ml of Tris buffer solution, Tris buffer are molten
Liquid pH=8.4 obtains surface amination modified polystyrene class medical catheter.
It is put into ethyl acetate solvent 2. surface amination modified polystyrene class medical catheter is several, ethyl acetate volume
It is 20ml, then adds in 0.05g/ml 4-dimethylaminopyridine, 0.08g/ml triethylamines, 0.2g/ml bromo isobutyl acylbromides,
It after reacting for 24 hours at room temperature, is dried in vacuo after ethyl alcohol cleaning, obtaining surface has the polystyrene type medical catheter of initiator.
3. surface has, the polystyrene type medical catheter of initiator is several, 4g (3- acrylamide propyls) trimethyl ammonia chloride
Ammonium, 4.5g methacrylates epsilon-polylysine (molecular weight 1600), 4.8g methacrylate polyethylene glycol (molecular weight
1000), using copper bromide/copper/N, N, N', N, ' N "-five methyl diethylentriamine is catalytic ligand system, the matter of copper bromide
Amount is 29.1mg, and the quality of copper is 30mg, N, N, N', N, ' the quality of N "-five methyl diethylentriamine is 46mg, reaction temperature
50 DEG C of degree, reaction dissolvent are methanol/water mixed solvent, and the volume of mixed solvent is 10ml, and the volume ratio of methanol/water is 1/1, instead
Between seasonable for 24 hours, ethyl alcohol cleaning, vacuum drying.
Fig. 9 and Figure 10 is in 4 gained coating surface culture methicillin-resistant staphylococcus grape ball of the embodiment of the present invention respectively
Bacterium and vancomycin enterococcus (bacterial concentration 106CFU/ml) shows fluorescent microscopy images after 24h.Compared with Fig. 1, Fig. 2, figure
9 are reduced with the coating surface bacterial number of Figure 10, show that 1 gained coating of the embodiment of the present invention has certain antibiotic property
Energy.
Embodiment 5
One kind prepares overriding resistance bacterium coating on medical catheter surface, includes the following steps：
1. by the several Tris buffer solution being immersed in containing dopamine of polyvinyl chloride medical conduit, at 37 DEG C
For 24 hours, then distilled water cleans for lower reaction, vacuum drying.A concentration of 4mg/ml of Tris buffer solution, Tris buffer are molten
Liquid pH=8.4 obtains surface amination modified polyvinyl chloride alkenes medical catheter.
It is put into ethyl acetate solvent 2. surface amination modified polyvinyl chloride alkenes medical catheter is several, ethyl acetate volume
It is 20ml, then adds in 0.05g/ml 4-dimethylaminopyridine, 0.08g/ml triethylamines, 0.2g/ml bromo isobutyl acylbromides,
It after reacting for 24 hours at room temperature, is dried in vacuo after ethyl alcohol cleaning, obtaining surface has the polyvinyl chloride medical conduit of initiator.
3. surface has, the polyvinyl chloride medical conduit of initiator is several, and 3g methacrylates gather methylene hydrochloric acid
(molecular weight 1000), 4.5g methacrylates epsilon-polylysine (molecular weight 4000), 4.8g methacrylate polyethylene glycol
(molecular weight 1000), using copper bromide/copper/N, N, N', N, ' N "-five methyl diethylentriamine is catalytic ligand system, bromination
The quality of copper is 29.1mg, and the quality of copper is 30mg, N, N, N', N, ' the quality of N "-five methyl diethylentriamine is 46mg,
50 DEG C of reaction temperature, reaction dissolvent are methanol/water mixed solvent, and the volume of mixed solvent is 10ml, and the volume ratio of methanol/water is
1/1, the reaction time, ethyl alcohol cleaning was dried in vacuo for 24 hours.
Figure 11 and Figure 12 is in 1 gained coating surface culture methicillin-resistant staphylococcus grape ball of the embodiment of the present invention respectively
Bacterium and vancomycin enterococcus (bacterial concentration 106CFU/ml) shows fluorescent microscopy images after 24h.Compared with Fig. 1, Fig. 2, figure
11 are reduced with the coating surface bacterial number of Figure 12, show that 1 gained coating of the embodiment of the present invention has certain antibiotic property
Energy.
The above is only the section Example of the present invention, not do limitation in any form to the present invention, it is every according to
According to any simple modification that the technical spirit of the present invention makees above-described embodiment, equivalent variations and modification belong to the present invention
In the range of technical solution.
Claims (10)
- A kind of 1. method for preparing overriding resistance bacterium coating on medical catheter surface, which is characterized in that include the following steps：(1) medical catheter surface is pre-processed, obtains amination or hydroxylated medical catheter；(2) initiator is fixed on the surface of amination or hydroxylating medical catheter, obtaining surface has the medical catheter of initiator；(3) there is the surface grafting of the medical catheter of initiator on surface with the organic molecule that carries positive charge or with positive electricity The polymer of lotus is monomer, using methacrylate polyethylene glycol as crosslinking agent, causes-supplement reduction atom transfer using surface The chemical graft method of free radical finally prepares polycation layer antimicrobial coating.
- 2. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： The medical catheter is high molecular material class medical catheter or silicates medical catheter.
- 3. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 2, it is characterised in that： The high molecular material class medical catheter is polyolefins conduit, polyvinyl chloride conduit, silica gel catheter, polystyrene.
- 4. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 2, it is characterised in that： The silicates medical catheter is grass tube or quartz conduit.
- 5. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： Medical catheter surface is pre-processed in step (1), is included the following steps：Medical catheter is immersed in containing dopamine It in Tris buffer solution, impregnates, wash, is dry, the temperature of Tris buffer solution is 37 DEG C, Tris buffer solution Concentration be 4mg/ml, the pH value of Tris buffer solution is 8.4.
- 6. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： Initiator is fixed on the surface of medical catheter in step (2) after the pre-treatment, is included the following steps：Pretreated medical Conduit is put into ethyl acetate, is then added in 4-dimethylaminopyridine, triethylamine, bromo isobutyl acylbromide, is reacted at room temperature for 24 hours Afterwards, it washs, is dry, the 4-dimethylaminopyridine concentration is 0.05g/ml, and triethylamine concentration is 0.08g/ml bromo isobutyryls The concentration of bromine is 0.2g/ml.
- 7. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： The polymer with positive charge is (3- acrylamide propyls) trimethyl ammonium chloride, dimethyl diallyl ammonium chloride, first Base quaternary ammonium acrylate polyethyleneimine, methacrylate epsilon-polylysine or methacrylate gather methylene hydrochloric acid, The concentration of the polymer with positive charge is 1.45mol/L.
- 8. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： The molecular weight of the polymer with positive charge is 800-4000.
- 9. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface according to claim 1, it is characterised in that： The molecular weight of the methacrylate polyethylene glycol is 700-4000.
- 10. a kind of method for preparing overriding resistance bacterium coating on medical catheter surface, feature exist according to claim 1 In：In the chemical graft method of step (3), include the following steps：With copper bromide/copper/N, N, N', N, ' N "-pentamethyl two is sub- Ethyl triamine is catalytic ligand system, monomer/copper bromide/copper/N, N, N', N, ' N "-five methyl diethylentriamine mole matches Than being 100/1/1/1, using the mixed solution of methanol/water as reaction dissolvent, step (2) treated medical catheter is several, monomer, Crosslinking agent reacts for 24 hours with catalytic ligand system in reaction dissolvent at 50 DEG C, is washed out, dries.
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Cited By (5)
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| CN109535356A (en) * | 2018-11-26 | 2019-03-29 | 迈凯特殊材料（苏州工业园区）有限公司 | The method of dimethyl diallyl ammonium halide is grafted on a kind of matrix |
| WO2020181857A1 (en) * | 2019-03-11 | 2020-09-17 | 脉通医疗科技(嘉兴)有限公司 | Medical tube and preparation method therefor |
| CN113082300A (en) * | 2021-04-06 | 2021-07-09 | 西南交通大学 | Antibacterial and anticoagulant coating, preparation method and application thereof |
| CN113842507A (en) * | 2021-07-19 | 2021-12-28 | 浙江大学 | Polyelectrolyte hydrogel coating with super-strong substrate adhesion performance and preparation method thereof |
| CN114904060A (en) * | 2022-05-30 | 2022-08-16 | 浙江大学 | Medical catheter containing anti-adhesion layer and preparation method thereof |
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| CHAO ZHOU等: "In Vivo Anti-Biofilm and Anti-Bacterial Non-Leachable Coating Thermally Polymerized on Cylindrical Catheter", 《ACS APPLIED MATERIALS & INTERFACES》 * |
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| WO2020181857A1 (en) * | 2019-03-11 | 2020-09-17 | 脉通医疗科技(嘉兴)有限公司 | Medical tube and preparation method therefor |
| CN113082300A (en) * | 2021-04-06 | 2021-07-09 | 西南交通大学 | Antibacterial and anticoagulant coating, preparation method and application thereof |
| CN113842507A (en) * | 2021-07-19 | 2021-12-28 | 浙江大学 | Polyelectrolyte hydrogel coating with super-strong substrate adhesion performance and preparation method thereof |
| CN113842507B (en) * | 2021-07-19 | 2022-04-29 | 浙江大学 | Polyelectrolyte hydrogel coating with super-strong substrate adhesion performance and preparation method thereof |
| WO2023000713A1 (en) * | 2021-07-19 | 2023-01-26 | 浙江大学 | Strong-adhesive polyelectrolyte hydrogel coating and preparation method therefor |
| CN114904060A (en) * | 2022-05-30 | 2022-08-16 | 浙江大学 | Medical catheter containing anti-adhesion layer and preparation method thereof |
| CN114904060B (en) * | 2022-05-30 | 2023-04-28 | 浙江大学 | Medical catheter containing anti-adhesion layer and preparation method thereof |
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