CN108158847A - A kind of shower cream containing candida utili callose - Google Patents

A kind of shower cream containing candida utili callose Download PDF

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Publication number
CN108158847A
CN108158847A CN201810110925.XA CN201810110925A CN108158847A CN 108158847 A CN108158847 A CN 108158847A CN 201810110925 A CN201810110925 A CN 201810110925A CN 108158847 A CN108158847 A CN 108158847A
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China
Prior art keywords
candida utili
cell
callose
precipitation
shower cream
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CN201810110925.XA
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Chinese (zh)
Inventor
马霞
刘蓝天
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上海应用技术大学
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Priority to CN201810110925.XA priority Critical patent/CN108158847A/en
Publication of CN108158847A publication Critical patent/CN108158847A/en

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/72Cosmetics or similar toilet preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/72Cosmetics or similar toilet preparations characterised by the composition containing organic macromolecular compounds
    • A61K8/73Polysaccharides
    • A61K8/735Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/92Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K8/00Cosmetics or similar toilet preparations
    • A61K8/18Cosmetics or similar toilet preparations characterised by the composition
    • A61K8/96Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toilet preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILET PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/805Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Abstract

The present invention provides a kind of shower creams containing candida utili β D glucans, count by weight percentage, by candida utili β D glucans 0.1% 1%, glycerine 10% 20%, olive oil 10% 20%, shea butter 2% 8%, Sodium Hyaluronate 0.1% 0.8%, matrimony vine root extract 5% 15%, licorice root extract 3% 10%, whorlleaf stonecrop herb extracting solution 1% 5%, the distilled water composition of surplus.The processing steps such as candida utili β D glucans of the present invention are obtained by cell, induction self-dissolving, high-temp extracting, high pressure microjet, complex enzyme hydrolysis, degreasing, freeze-drying prepare gained, it can add the shower cream being prepared into preferable moisturizing and anti-oxidation efficacy with moisturizing and oxidation resistant effect.There is moisturizing, the reparation of wound, radioresistance, anti-oxidant and other effects by the shower cream that yeast β D glucans are prepared.

Description

A kind of shower cream containing candida utili callose

Technical field

The invention belongs to daily chemicals fields, are related to a kind of skin care item, specifically refer to a kind of to contain candida utili β-D- The shower cream of glucan.

Background technology

Candida utili is can be used as food for one kind by many national authentications together with brewer's yeast, kluyveromyces The microorganism available for health food that the microorganism of additive and China Health department allow.Yeast beta-dextran is ferment The structural polysaccharide of mother cell wall has different functional characteristics, is conducive to antimicrobial antiphlogistic reaction;Promote the absorption of mould toxin;Subtract The effects that lacking the cholesterol particles in low-density lipoprotein;Also have anticancer, anti-mutagenesis, it is anti-oxidant and promote wound healing, The characteristic of moisturizing.

Skin is the organ of human body maximum, and skin moisture-keeping and antioxidation are most important for skin care.It is protected containing natural The shower cream of wet and anti-oxidation function yeast beta-dextran can help skin to restore deep moisturizing function, effective stimulus skin The natural generation of internal natural moisturizing factor, the effect of returning equilibrium state, moisten moisturizing deeply, play deep layer water lock moisturizing.

Invention content

For above-mentioned technical problem of the prior art, gather the present invention provides one kind containing candida utili β-D- Portugals The shower cream of sugar, this shower cream containing candida utili callose will solve makeup of the prior art Product add excessive artificial chemistry ingredient and cause moistening effect and antioxidant effect undesirable and have the technical issues of side effect.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:

Candida utili callose 0.1%-1%;

Glycerine 10%-20%;

Olive oil 10%-20%;

Shea butter 2%-8%;

Sodium Hyaluronate 0.1%-0.8%;

Matrimony vine root extract 5%-15%;

Licorice root extract 3%-10%;

Whorlleaf stonecrop herb extracting solution 1%-5%;

Surplus is distilled water.

Further, the mass percent of each raw material is:

Candida utili callose 0.8%;

Glycerine 14%;

Olive oil 11%;

Shea butter 8%;

Sodium Hyaluronate 0.7%;

Matrimony vine root extract 10%;

Licorice root extract 9%;

Whorlleaf stonecrop herb extracting solution 5%;

Surplus is distilled water.

Specifically, the effective ingredient of matrimony vine root extract is flavones, there is bacteriostasis, flavones in matrimony vine root extract Mass percentage content is not less than 20%;The effective ingredient of licorice root extract is that glycyrrhizic acid, liquiritin etc. are made with antibacterial whitening With the mass percentage content of active ingredient glycyrrhizic acid and liquiritin is not less than 50% in licorice root extract;Whorlleaf stonecrop herb extracting solution In effective ingredient be biflavone, there is promoting blood circulation bacteriostasis, the mass percentage content of biflavone is not in whorlleaf stonecrop herb extracting solution Less than 30%.

Further, the preparation method of the candida utili callose includes the following steps:

1) obtain candida utili cell the step of, candida utili cultivated in deproteinized murphy juice to 10 ~ 20h is then centrifuged for, then is rinsed with deionized water to get to candida utili cell;

2) induction self-dissolving is carried out to candida utili cell the step of, by step 1)The candida utili cell of acquisition Be added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0,55-60 DEG C, under conditions of 100 ~ 140rpm oscillation from Molten 20 ~ 30h is centrifuged after self-dissolving, and is rinsed with deionized water;

3) the step of high-temp extracting, by step 2)Obtain the phosphate buffer that candida utili cell adds in pH6.0 In, in phosphate buffer, the mass percent concentration of yeast cells is 10%, candida utili cell is suspended, then A diameter of 0.5cm beades are added in, carry out high-temp extracting, 1 ~ 3h of extraction time, temperature are 100 ~ 140 DEG C, centrifugation, use deionization Water washing obtains enrichment precipitation;

4) the step of high pressure microjet makes step 3 with deionized water)Enrichment precipitation suspend, in 120MPa pressure conditions Lower high pressure broken wall centrifuges to obtain cell wall precipitation at recycling 2 ~ 5 times, 4 DEG C;

5) the step of complex enzyme hydrolysis, by step 4)It obtains candida utili cell wall and is made into quality percentage with deionized water Specific concentration is 10% cell wall suspension, adds in the neutral proteinase and cellulase of candida utili cell wall quality 1%, The mass ratio of neutral proteinase and cellulase is 1:1, enzymolysis time for 1 ~ 3h, 50 ~ 60 DEG C of temperature, centrifugation, precipitation spend from Sub- water washing, is precipitated;

6) the step of degreasing, by step 5)Precipitation be added in anhydrous ethanol solvent, extracted, obtained precipitation is used Water washing centrifuges, and freeze-drying obtains candida utili callose.

Further, the deposit number CICC1769 of the candida utili.

Further, step 2)The centrifugal force of middle centrifugation is 3000g, and temperature is 4 DEG C.

The present invention provides a kind of preparation method of the shower cream containing candida utili callose, the production proteins Candida callose is obtained by cell, induction self-dissolving, high-temp extracting, high pressure microjet, complex enzyme hydrolysis, degreasing, cold Dry wait obtained by processing steps preparation, with moisturizing and oxidation resistant effect is lyophilized.The present invention is using candida utili as raw material The callose of production can be used in food, feed, cosmetics and aquaculture;Using discarded murphy juice as production The culture medium of protein Candida can mitigate pollution of the waste liquid to environment, be of great significance to environmental protection;Extraction process The degradation that the conventional methods such as acid-base reagent bring product is avoided, whole process is raw materials used cheap, can advise greatly Mould application.

A kind of candida utili strain of the present invention is micro- life available for health food that China Health department allows Object, therefore candida utili callose has safe and reliable handling characteristics.

The method that callose is extracted in a kind of cell wall from candida utili of the present invention, compared to traditional extraction Method, as a result of a kind of relatively mild extraction callose method of condition, operating process is simple and practicable, quick, heavier The degradation for being that of avoiding acid-base reagent and being brought to product wanted;It is prepared by the callose extracted by this method Obtained shower cream can avoid other excessive artificial chemical reagent of addition, so as to avoid there is the phenomenon that side effects such as allergy.

The present invention is compared with prior art, and technological progress is significant.The present invention is from candida utili cell wall The callose of extraction is pure natural substance, has the bioactivity such as moisturizing, the reparation of wound, radioresistance, anti-oxidant, The shower cream being prepared has corresponding moisturizing and anti-oxidant and other effects.

Specific embodiment

The present invention is expanded on further, but be not intended to limit the present invention below by specific embodiment.

Candida utili strain candida utili used in various embodiments of the present invention(Candidautilis), protect Number CICC1769 is hidden, in Chinese industrial Microbiological Culture Collection administrative center preservation, preservation organization address:Chaoyang District, Beijing City No. 6 building in winebibber's bridge Road 24, postcode:100015, it is any to buy per capita.

Embodiment 1

Candida utili callose, is prepared by a method comprising the following steps:

1) candida utili is cultivated into 14h in deproteinized murphy juice, is then centrifuged for, then with deionized water rinse to get to Candida utili cell;Candida utili used(Candidautilis), deposit number CICC1769;

2) the candida utili cell obtained is added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0, 55-60 DEG C, oscillation self-dissolving in centrifugal force is 3000g after self-dissolving, temperature is to be centrifuged at 4 DEG C, is used in combination for 24 hours under conditions of 120rpm Deionized water is rinsed;

3) candida utili cell will be obtained to add in the phosphate buffer of pH6.0, in phosphate buffer, yeast is thin The mass percent concentration of born of the same parents is 10%, and candida utili cell is suspended, then adds in a diameter of 0.5cm beades, feed liquid Than 1:15, high-temp extracting is carried out, extraction time 2h, temperature are 120 DEG C, centrifugation, are washed with deionized, and obtain enrichment precipitation;

4) enrichment precipitation is made to suspend with deionized water, the high pressure broken wall under 120MPa pressure conditions recycles 3 times, and temperature is at 4 DEG C Centrifuge to obtain cell wall precipitation;

5) cell wall precipitation will be obtained and is made into the cell wall suspension that mass percent concentration is 10% with deionized water, add in production 1% neutral proteinase and cellulase of protein candida cell wall quality, enzymolysis time 2h, 55 DEG C of temperature, centrifugation, sink Shallow lake is washed with deionized, and is precipitated;

6) precipitation is added in anhydrous ethanol solvent, is extracted repeatedly, obtained precipitation is washed with water, centrifugation, and freezing is dry Dry, it is callose product to obtain pale yellow powder.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:Candida utili callose 0.8%, glycerine 14%, olive oil 11%, shea butter 8%, Sodium Hyaluronate 0.7%, Chinese holly Qi root extract 10%, licorice root extract 9%, whorlleaf stonecrop herb extracting solution 5%, surplus are distilled water.

The preparation method of above-mentioned shower cream is that the substance that will be weighed according to mass percent uniformly mixes.Similarly hereinafter.

Embodiment 2

Candida utili callose, is prepared by a method comprising the following steps:

1) candida utili is cultivated into 14h in deproteinized murphy juice, is then centrifuged for, then with deionized water rinse to get to Candida utili cell;Candida utili used(Candidautilis), deposit number CICC1769;

2) the candida utili cell obtained is added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0, 55-60 DEG C, oscillation self-dissolving in centrifugal force is 3000g after self-dissolving, temperature is to be centrifuged at 4 DEG C, is used in combination for 24 hours under conditions of 120rpm Deionized water is rinsed;

3) candida utili cell will be obtained to add in the phosphate buffer of pH6.0, in phosphate buffer, yeast is thin The mass percent concentration of born of the same parents is 10%, and candida utili cell is suspended, then adds in a diameter of 0.5cm beades, feed liquid Than 1:15, high-temp extracting is carried out, extraction time 2h, temperature are 120 DEG C, centrifugation, are washed with deionized, and obtain enrichment precipitation;

4) enrichment precipitation is made to suspend with deionized water, the high pressure broken wall under 120MPa pressure conditions recycles 3 times, and temperature is at 4 DEG C Centrifuge to obtain cell wall precipitation;

5) cell wall precipitation will be obtained and is made into the cell wall suspension that mass percent concentration is 10% with deionized water, add in production 1% neutral proteinase and cellulase of protein candida cell wall quality, enzymolysis time 2h, 55 DEG C of temperature, centrifugation, sink Shallow lake is washed with deionized, and is precipitated;

6) precipitation is added in anhydrous ethanol solvent, is extracted repeatedly, obtained precipitation is washed with water, centrifugation, and freezing is dry Dry, it is callose product to obtain pale yellow powder.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:Candida utili callose 0.5%, glycerine 12%, olive oil 14%, shea butter 5%, Sodium Hyaluronate 0.5%, Chinese holly Qi root extract 12%, licorice root extract 3%, whorlleaf stonecrop herb extracting solution 3%, surplus are distilled water.

Embodiment 3

Candida utili callose, is prepared by a method comprising the following steps:

1) candida utili is cultivated into 14h in deproteinized murphy juice, is then centrifuged for, then with deionized water rinse to get to Candida utili cell;Candida utili used(Candidautilis), deposit number CICC1769;

2) the candida utili cell obtained is added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0, 55-60 DEG C, oscillation self-dissolving in centrifugal force is 3000g after self-dissolving, temperature is to be centrifuged at 4 DEG C, is used in combination for 24 hours under conditions of 120rpm Deionized water is rinsed;

3) candida utili cell will be obtained to add in the phosphate buffer of pH6.0, in phosphate buffer, yeast is thin The mass percent concentration of born of the same parents is 10%, and candida utili cell is suspended, then adds in a diameter of 0.5cm beades, feed liquid Than 1:15, high-temp extracting is carried out, extraction time 2h, temperature are 120 DEG C, centrifugation, are washed with deionized, and obtain enrichment precipitation;

4) enrichment precipitation is made to suspend with deionized water, the high pressure broken wall under 120MPa pressure conditions recycles 3 times, and temperature is at 4 DEG C Centrifuge to obtain cell wall precipitation;

5) cell wall precipitation will be obtained and is made into the cell wall suspension that mass percent concentration is 10% with deionized water, add in production 1% neutral proteinase and cellulase of protein candida cell wall quality, enzymolysis time 2h, 55 DEG C of temperature, centrifugation, sink Shallow lake is washed with deionized, and is precipitated;

6) precipitation is added in anhydrous ethanol solvent, is extracted repeatedly, obtained precipitation is washed with water, centrifugation, and freezing is dry Dry, it is callose product to obtain pale yellow powder.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:Candida utili callose 0.2%, glycerine 13%, olive oil 18%, shea butter 2%, Sodium Hyaluronate 0.3%, Chinese holly Qi root extract 7%, licorice root extract 6%, whorlleaf stonecrop herb extracting solution 1%, surplus are distilled water.

Embodiment 4

Candida utili callose, is prepared by a method comprising the following steps:

1) candida utili is cultivated into 14h in deproteinized murphy juice, is then centrifuged for, then with deionized water rinse to get to Candida utili cell;Candida utili used(Candidautilis), deposit number CICC1769;

2) the candida utili cell obtained is added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0, 55-60 DEG C, oscillation self-dissolving in centrifugal force is 3000g after self-dissolving, temperature is to be centrifuged at 4 DEG C, is used in combination for 24 hours under conditions of 120rpm Deionized water is rinsed;

3) candida utili cell will be obtained to add in the phosphate buffer of pH6.0, in phosphate buffer, yeast is thin The mass percent concentration of born of the same parents is 10%, and candida utili cell is suspended, then adds in a diameter of 0.5cm beades, feed liquid Than 1:15, high-temp extracting is carried out, extraction time 2h, temperature are 120 DEG C, centrifugation, are washed with deionized, and obtain enrichment precipitation;

4) enrichment precipitation is made to suspend with deionized water, the high pressure broken wall under 120MPa pressure conditions recycles 3 times, and temperature is at 4 DEG C Centrifuge to obtain cell wall precipitation;

5) cell wall precipitation will be obtained and is made into the cell wall suspension that mass percent concentration is 10% with deionized water, add in production 1% neutral proteinase and cellulase of protein candida cell wall quality, enzymolysis time 2h, 55 DEG C of temperature, centrifugation, sink Shallow lake is washed with deionized, and is precipitated;

6) precipitation is added in anhydrous ethanol solvent, is extracted repeatedly, obtained precipitation is washed with water, centrifugation, and freezing is dry Dry, it is callose product to obtain pale yellow powder.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:Candida utili callose 0.7%, glycerine 15%, olive oil 15%, shea butter 4%, Sodium Hyaluronate 0.4%, Chinese holly Qi root extract 8%, licorice root extract 5%, whorlleaf stonecrop herb extracting solution 2%, surplus are distilled water.

Embodiment 5

Candida utili callose, is prepared by a method comprising the following steps:

1) candida utili is cultivated into 14h in deproteinized murphy juice, is then centrifuged for, then with deionized water rinse to get to Candida utili cell;Candida utili used(Candidautilis), deposit number CICC1769;

2) the candida utili cell obtained is added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0, 55-60 DEG C, oscillation self-dissolving in centrifugal force is 3000g after self-dissolving, temperature is to be centrifuged at 4 DEG C, is used in combination for 24 hours under conditions of 120rpm Deionized water is rinsed;

3) candida utili cell will be obtained to add in the phosphate buffer of pH6.0, in phosphate buffer, yeast is thin The mass percent concentration of born of the same parents is 10%, and candida utili cell is suspended, then adds in a diameter of 0.5cm beades, feed liquid Than 1:15, high-temp extracting is carried out, extraction time 2h, temperature are 120 DEG C, centrifugation, are washed with deionized, and obtain enrichment precipitation;

4) enrichment precipitation is made to suspend with deionized water, the high pressure broken wall under 120MPa pressure conditions recycles 3 times, and temperature is at 4 DEG C Centrifuge to obtain cell wall precipitation;

5) cell wall precipitation will be obtained and is made into the cell wall suspension that mass percent concentration is 10% with deionized water, add in production 1% neutral proteinase and cellulase of protein candida cell wall quality, enzymolysis time 2h, 55 DEG C of temperature, centrifugation, sink Shallow lake is washed with deionized, and is precipitated;

6) precipitation is added in anhydrous ethanol solvent, is extracted repeatedly, obtained precipitation is washed with water, centrifugation, and freezing is dry Dry, it is callose product to obtain pale yellow powder.

The present invention provides a kind of shower cream containing candida utili callose, the mass percents of each raw material For:Candida utili callose 0.6%, glycerine 18%, olive oil 12%, shea butter 6%, Sodium Hyaluronate 0.6%, Chinese holly Qi root extract 14%, licorice root extract 4%, whorlleaf stonecrop herb extracting solution 4%, surplus are distilled water.

Above-described embodiment product shows that average moisturizing rate is 68.8% through external moisturizing experiment, and anti-oxidant experiment shows pair DPPH free radical scavenging activities average out to 43.9%, and have the effect for promoting the generation of endogenous natural moisturizing factor.

The above is merely to illustrate present disclosure, and in addition to this, the present invention also has other embodiment, in every case Those skilled in the art are because of technical inspiration involved in the present invention, and the technology that equivalent replacement or equivalent deformation mode is used to be formed Scheme is all fallen in protection scope of the present invention.

Claims (5)

1. a kind of shower cream containing candida utili callose, which is characterized in that the mass percent of each raw material is:
Candida utili callose 0.1%-1%;
Glycerine 10%-20%;
Olive oil 10%-20%;
Shea butter 2%-8%;
Sodium Hyaluronate 0.1%-0.8%;
Matrimony vine root extract 5%-15%;
Licorice root extract 3%-10%;
Whorlleaf stonecrop herb extracting solution 1%-5%;
Surplus is distilled water.
A kind of 2. shower cream containing candida utili callose as described in claim 1, which is characterized in that each original The mass percent of material is:
Candida utili callose 0.8%;
Glycerine 14%;
Olive oil 11%;
Shea butter 8%;
Sodium Hyaluronate 0.7%;
Matrimony vine root extract 10%;
Licorice root extract 9%;
Whorlleaf stonecrop herb extracting solution 5%;
Surplus is distilled water.
3. a kind of shower cream containing candida utili callose as described in claim 1, which is characterized in that described The preparation method of candida utili callose include the following steps:
1)One the step of obtaining candida utili cell, candida utili cultivated in deproteinized murphy juice to 10 ~ 20h is then centrifuged for, then is rinsed with deionized water to get to candida utili cell;
2)One the step of induction self-dissolving is carried out to candida utili cell, by step 1)The candida utili cell of acquisition Be added to the sodium chloride solution that mass percent concentration is 5%, in pH7.0,55-60 DEG C, under conditions of 100 ~ 140rpm oscillation from Molten 20 ~ 30h is centrifuged after self-dissolving, and is rinsed with deionized water;
3)The step of one high-temp extracting, by step 2)Obtain the phosphate buffer that candida utili cell adds in pH6.0 In, in phosphate buffer, the mass percent concentration of yeast cells is 10%, candida utili cell is suspended, then A diameter of 0.5cm beades are added in, carry out high-temp extracting, 1 ~ 3h of extraction time, temperature are 100 ~ 140 DEG C, centrifugation, use deionization Water washing obtains enrichment precipitation;
4)The step of one high pressure microjet, makes step 3 with deionized water)Enrichment precipitation suspend, in 120MPa pressure conditions Lower high pressure broken wall centrifuges to obtain cell wall precipitation at recycling 2 ~ 5 times, 4 DEG C;
5)The step of one complex enzyme hydrolysis, by step 4)It obtains candida utili cell wall and is made into quality percentage with deionized water Specific concentration is 10% cell wall suspension, adds in the neutral proteinase and cellulase of candida utili cell wall quality 1%, The mass ratio of neutral proteinase and cellulase is 1:1, enzymolysis time for 1 ~ 3h, 50 ~ 60 DEG C of temperature, centrifugation, precipitation spend from Sub- water washing, is precipitated;
6)The step of one degreasing, by step 5)Precipitation be added in anhydrous ethanol solvent, extracted, obtained precipitation is used Water washing centrifuges, and freeze-drying obtains candida utili callose.
4. a kind of shower cream containing candida utili callose as described in claim 1, which is characterized in that described Candida utili deposit number CICC1769.
A kind of 5. shower cream containing candida utili callose as described in claim 1, which is characterized in that step 2)The centrifugal force of middle centrifugation is 3000g, and temperature is 4 DEG C.
CN201810110925.XA 2018-02-05 2018-02-05 A kind of shower cream containing candida utili callose CN108158847A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106265259A (en) * 2016-08-24 2017-01-04 彭友莲 A kind of whitening type bath gel and preparation method thereof
CN106397628A (en) * 2016-10-11 2017-02-15 上海应用技术大学 Method for extracting beta-D-glucan from cell walls of candida utilis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106265259A (en) * 2016-08-24 2017-01-04 彭友莲 A kind of whitening type bath gel and preparation method thereof
CN106397628A (en) * 2016-10-11 2017-02-15 上海应用技术大学 Method for extracting beta-D-glucan from cell walls of candida utilis

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