CN108152498A - A kind of inactivation method of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine - Google Patents
A kind of inactivation method of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine Download PDFInfo
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- CN108152498A CN108152498A CN201611100655.1A CN201611100655A CN108152498A CN 108152498 A CN108152498 A CN 108152498A CN 201611100655 A CN201611100655 A CN 201611100655A CN 108152498 A CN108152498 A CN 108152498A
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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Abstract
The invention discloses a kind of inactivation methods of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine.The present invention relates to biomedicine fields, and in particular to a kind of inactivation method of inspection for being directed to bovine viral diarrhea virus, infectious bovine rhinotrachetis virus, bovine parainfluenza virus, Bovine Respiratory Syncytial viral inactivation vaccine includes the following steps：1）Virus liquid after inactivation adsorbs 40 60min on suitable cell, during which shakes 23 times；2）After absorption, the cell maintenance medium that certain volume is added in cell spinner bottle continues to cultivate, and observes cytopathy, for the virus liquid of acellular lesion, harvests cell culture fluid, and freeze thawing 23 times；3）10 15ml of the cell culture fluid after freeze thawing is taken, is inoculated on suitable cell and adsorbs, continues to cultivate.It is observed and fluoroscopic examination, such as without specificity fluorescent, then shows that inactivation is complete.Technical solution disclosed in the present invention ensure that antigen concentration and viral infection time.It examines the recall rate of virus high through the present invention inactivation method of inspection, can effectively ensure the bio-safety of inactivated vaccine.
Description
Technical field
The present invention relates to biomedicine fields, and in particular to one kind is suitable for bovine viral diarrhoea（BVDV）, ox infectiousness
Rhinotracheitis（IBRV）, bovine parainfluenza（PIV3）, Bovine Respiratory Syncytial virosis（BRSV）The inactivation inspection party of inactivated vaccine
Method belongs to Veterinary Medicine biological field.
Background technology
Whether vaccine immunity is the important means of prevention and control epidemic disease, survive by pathogenic microorganism in vaccine, vaccine is divided into and is gone out
Live vaccine and live vaccine.Inactivated vaccine be by pathogenic microorganism chemically or physical method makes micro- life used in manufacture vaccine
Activity, reproductive capacity or the pathogenicity of object disappear, but still preserve the immunogenicity of corresponding antigens.
It is one of technique important in inactivated vaccine preparation process that inactivation, which is examined, is to ensure that the important of vaccine bio-safety is arranged
It applies, therefore, the effective method of inspection that inactivates has great importance to the security application of vaccine.Currently, different inactivated vaccine roots
According to the characteristic of cause of disease, there is the different inactivation methods of inspection, but its it is most basic be will by it is easy, quickly determine whether to inactivate
Completely, with ensure environmental organism safety, vaccine dissipate poison.
Since the characteristic of cause of disease in different inactivated vaccines is different, thus need according to the growth characteristic of cause of disease and inactivator
Characteristic Design forms the different inactivation methods of inspection.For different virus, method does not have migration and applicability.For example
The inactivation method of inspection of pig circular ring virus inactivated vaccine is not particularly suited for bovine viral diarrhea virus and infectious bovine rhinotrachetis
The inactivation of virus is examined.
Invention content
It is suitable for bovine viral diarrhoea, infectious bovine rhinotrachetis, bovine parainfluenza, ox the purpose of the present invention is design is a kind of
The inactivation method of inspection of respiratory syncytial virus (RSV) inactivated vaccine.
In order to realize the purpose, the invention discloses a kind of inactivation method of inspection, include the following steps：
1）According to needs to be detected, select suitable for bovine viral diarrhea virus, infectious bovine rhinotrachetis virus, bovine parainfluenza
Or the cell line of Bovine Respiratory Syncytial virus suitable growth carries out recovery culture, wherein alternative cell line includes ox
Kidney cell line（MDBK cell lines）, bull testis cell（BT cell lines）, nose of an ox first osteocyte system（BT cell lines）, Vero cells
System, BHK21 cell lines, ox primary cell, mdck cell system, cattle uterus cell line（NCL-1 cell lines）, NCL-1 cell lines, rabbit
Kidney primary cell（CRL-1414）, rabbit kidney cell（LLC-RK1）.
2）2 are selected to grow up to good cell monolayer, density discards cell training up to the rolling bottle of the appropriate volume of 80%-90%
Nutrient solution takes the virus liquid 20-30ml after inactivation to be inoculated in cell spinner bottle, puts 37 DEG C, containing 5%CO2In incubator, after making inactivation
Virus liquid 40-60min is adsorbed on aforementioned suitable cell, during which shake 2-3 times；
3）After absorption, the cell maintenance medium of certain volume is added in cell spinner bottle, cell spinner bottle is placed in 37 DEG C of CO2Culture
After continuing culture in case 3-4 days, cytopathy is observed, for the virus liquid of acellular lesion, harvests cell culture fluid, and freeze thawing
2-3 times；
4）The cell culture fluid 10-15ml after freeze thawing is taken, is inoculated in 175cm2It is the suitable of 80%-90% that square vase, which has grown to density,
On suitable cell after absorption 40-60min, nutrition maintaining liquid is added in cell spinner bottle, cell bottle is placed in 37 DEG C of CO2Incubator
In continue culture 3 days, for may occur in which the virus liquid of cytopathy, observing cytopathy, such as acellular lesion, then showing to inactivate
Completely；
5）For not occurring the virus liquid of cytopathy normally, culture solution is harvested, further carries out fluorescent staining.Wherein fluorescence contaminates
The mode of color is：The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in the 96 hole cell trainings for covering with individual layer suitable cell
It supports in plate, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, is placed in 37 DEG C of CO2In incubator
Continue culture 24 hours, culture solution is discarded, after cleaning 3 times with PBS, with 80% acetone and 20% methyl alcohol mixed liquor 0.1mL in -20 DEG C
Fixed 30min, after cleaning 3 times with PBS, is separately added into the monoclonal antibody for particular virus of working concentration fluorescent marker
0.1mL in 37 DEG C of wet box after effect 40min, is cleaned 2 times with PBS, is placed in fluorescence microscopy Microscopic observation result.Positive-virus pair
Unstressed configuration is answered according to hole and normal cell controls hole.Test hole is as without specificity fluorescent, then shown that inactivation is complete；Such as occur special
Property fluorescence, then inactivation is incomplete.
Further, the cell culture fluid, the cell maintenance medium addition in cell bottle are the 1/ of cell bottle capacity
8-1/15。
Further, the cell culture fluid is the DMEM added with newborn bovine serum, fetal calf serum or horse serum
Or MEM nutrient solutions；The cell maintenance medium is DMEM the or MEM nutrient solutions added with newborn bovine serum or fetal calf serum.
Further, in cell culture fluid, the additive amount of newborn bovine serum, fetal calf serum or horse serum is 5%-
10%；In cell maintenance medium, the additive amount of newborn bovine serum or fetal calf serum is 1%-3.5%.
It is worth it is specifically intended that not containing antibiotic in cell culture fluid disclosed in this invention and cell maintenance medium.
Compared to the prior art, the present invention technically has 2 important innovations：1）Inactivation inspection disclosed in this invention
Proved recipe method need not dilute inactivation of viruses liquid；2）It is carried out using the monoclonal antibody of fluorescent marker inactivation inspection energy specificity, straight
Sight distinguishes certain inactivation of virus situation that cell CPE does not occur.Therefore, the technical solution disclosed in the present invention not only ensure that
Antigen concentration and viral infection time, and examine the recall rate of virus high through the present invention inactivation method of inspection.The present invention realizes
The high accuracy that inactivation of virus is examined, can effectively improve the safety of inactivated vaccine.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art
Member it should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and form into
Row modifications or substitutions, but these modifications and replacement are each fallen in protection scope of the present invention.
If without Special Statement, reagent employed in the present invention etc. is commercial product.
The inactivation method of inspection of 1 bovine viral diarrhea virus inactivated vaccine of embodiment
Cell and cell culture fluid：MDBK cells, cell culture fluid are the DMEM nutrient solutions containing 6% newborn bovine serum；Cell maintains
Liquid is the DMEM nutrient solutions containing 3% horse serum.
1）It cultivates, the MDBK cell inoculation 3000ml rolling bottles of digestion until growing up to the good cell lists of 80%-90% 48 hours
Layer, discards cell culture fluid, and the bovine viral diarrhea virus liquid 25ml of the MDBK cell culture after inactivation is taken to be inoculated in cell and is turned
In bottle, 37 DEG C are put, containing 5%CO2In incubator, the virus liquid after inactivation is made to adsorb 40min on suitable cell, during which shake 2
It is secondary；
2）After absorption, the DMEM cell maintenance mediums that 175ml contains 3% horse serum are added in cell bottle, cell bottle is placed in 37 DEG C
CO2Continue in incubator culture 3 days after, observe cytopathy, then show to inactivate if any cytopathy it is unqualified, for without thin
The virus liquid of born of the same parents' lesion, then harvest cell culture fluid, and freeze thawing 2 times；
3）The cell culture fluid 15ml after above-mentioned freeze thawing is taken, is inoculated in 175cm2It is 80%-90%'s that square vase, which has grown to density,
MDBK cells on cells after absorption 40min, the cell maintenance medium containing 3% horse serum are added in cell bottle, cell bottle is placed in
37 DEG C of CO2Continue culture 3 days in incubator.Cytopathy, such as acellular lesion are observed, then shows that inactivation is complete.For just
Often do not occur the virus liquid of cytopathy, harvest culture solution, further carry out fluorescent staining.
4）The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in the 96 hole cell trainings for covering with individual layer MDBK cells
It supports in plate, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, is placed in 37 DEG C of CO2In incubator
Continue culture 24 hours, culture solution is discarded, after being cleaned with PBS, with the 0.1mL -20 DEG C of fixations of 80% acetone and 20% methyl alcohol mixed liquor
30min after being cleaned with PBS, is separately added into the BVDV1 type monoclonal fluorescence antibody 0.1mL of 2.5 μ g/ml, is acted in 37 DEG C of wet box
It after 40min, is cleaned 2 times with PBS, is placed in fluorescence microscopy Microscopic observation result.Positive-virus control wells and normal cell controls hole
Answer unstressed configuration.Test hole is as without specificity fluorescent, then shown that inactivation is complete；Such as there is specificity fluorescent, then inactivation is incomplete.
The inactivation method of inspection of 2 infectious bovine rhinotrachetis cell toxicant inactivated vaccine of embodiment
Cell and cell culture fluid：MDBK cells or BT cells, cell culture fluid are the MEM nutrient solutions containing 6% newborn bovine serum；
Cell maintenance medium is the MEM nutrient solutions containing 3% newborn bovine serum.
1）It cultivates, the MDBK cell inoculation 3000ml rolling bottles of digestion until growing up to the good cell lists of 80%-90% 48 hours
Layer, discards cell culture fluid, and the infectious bovine rhinotrachetis virus liquid 25ml of the MDBK cell culture after inactivation is taken to be inoculated in carefully
In dysuria with lower abdominal colic bottle, 37 DEG C are put, containing 5%CO2In incubator, the virus liquid after inactivation is made to adsorb 40min on suitable cell, is during which shaken
It shakes 2 times；
2）After absorption, the DMEM cell maintenance mediums that 175ml contains 3% horse serum are added in cell bottle, cell bottle is placed in 37 DEG C
CO2Continue in incubator culture 3 days after, observe cytopathy, then show to inactivate if any cytopathy it is unqualified, for without thin
The virus liquid of born of the same parents' lesion, then harvest cell culture fluid, and freeze thawing 2 times；
3）The cell culture fluid 15ml after above-mentioned freeze thawing is taken, is inoculated in 175cm2It is 80%-90%'s that square vase, which has grown to density,
MDBK cells on cells after absorption 40min, the cell maintenance medium containing 3% horse serum are added in cell bottle, cell bottle is placed in
37 DEG C of CO2Continue culture 3 days in incubator.Cytopathy, such as acellular lesion are observed, then shows that inactivation is complete.For just
Often do not occur the virus liquid of cytopathy, harvest culture solution, further carry out fluorescent staining.
4）The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in the 96 hole cell trainings for covering with individual layer MDBK cells
It supports in plate, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, is placed in 37 DEG C of CO2In incubator
Continue culture 24 hours, culture solution is discarded, after being cleaned with PBS, with the 0.1mL-20 DEG C of fixation of 80% acetone and 20% methyl alcohol mixed liquor
30min after being cleaned with PBS, is separately added into the IBRV monoclonal fluorescence antibodies 0.1mL of 2.5 μ g/ml, is acted in 37 DEG C of wet box
It after 40min, is cleaned 2 times with PBS, is placed in fluorescence microscopy Microscopic observation result.Positive-virus control wells and normal cell controls hole
Answer unstressed configuration.Test hole is as without specificity fluorescent, then shown that inactivation is complete；Such as there is specificity fluorescent, then inactivation is incomplete.
The inactivation method of inspection of 3 bovine parainfluenza type-3 cell toxicant inactivated vaccine of embodiment
Cell and cell culture fluid：MDBK cells or BT cells, cell culture fluid are the MEM nutrient solutions containing 6% newborn bovine serum；
Cell maintenance medium is the MEM nutrient solutions containing 3% newborn bovine serum.
1）It cultivates, the MDBK cell inoculation 3000ml rolling bottles of digestion until growing up to the good cell lists of 80%-90% 48 hours
Layer, discards cell culture fluid, and the infectious bovine rhinotrachetis virus liquid 25ml of the MDBK cell culture after inactivation is taken to be inoculated in carefully
In dysuria with lower abdominal colic bottle, 37 DEG C are put, containing 5%CO2In incubator, the virus liquid after inactivation is made to adsorb 40min on suitable cell, is during which shaken
It shakes 2 times；
2）After absorption, the DMEM cell maintenance mediums that 175ml contains 3% horse serum are added in cell bottle, cell bottle is placed in 37 DEG C
CO2Continue in incubator culture 3 days after, observe cytopathy, then show to inactivate if any cytopathy it is unqualified, for without thin
The virus liquid of born of the same parents' lesion, then harvest cell culture fluid, and freeze thawing 2 times；
3）The cell culture fluid 15ml after above-mentioned freeze thawing is taken, is inoculated in 175cm2It is 80%-90%'s that square vase, which has grown to density,
MDBK cells on cells after absorption 40min, the cell maintenance medium containing 3% horse serum are added in cell bottle, cell bottle is placed in
37 DEG C of CO2Continue culture 3 days in incubator.Cytopathy, such as acellular lesion are observed, then shows that inactivation is complete.For just
Often do not occur the virus liquid of cytopathy, harvest culture solution, further carry out fluorescent staining.
4）The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in the 96 hole cell trainings for covering with individual layer MDBK cells
It supports in plate, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, is placed in 37 DEG C of CO2In incubator
Continue culture 24 hours, culture solution is discarded, after being cleaned with PBS, with the 0.1mL-20 DEG C of fixation of 80% acetone and 20% methyl alcohol mixed liquor
30min after being cleaned with PBS, is separately added into the PI3 monoclonal fluorescence antibodies 0.1mL of 2.5 μ g/ml, is acted in 37 DEG C of wet box
After 40min, clear 2 times with PBS, it is placed in fluorescence microscopy Microscopic observation result.Positive-virus control wells and normal cell controls hole
Answer unstressed configuration.Test hole is as without specificity fluorescent, then shown that inactivation is complete；Such as there is specificity fluorescent, then inactivation is incomplete.
The inactivation method of inspection of 4 Bovine Respiratory Syncytial viral inactivation vaccine of embodiment
Cell and cell culture fluid：MDBK cells or BT cells, cell culture fluid are the MEM nutrient solutions containing 6% newborn bovine serum；
Cell maintenance medium is the MEM nutrient solutions containing 3% newborn bovine serum.
1）It cultivates, the MDBK cell inoculation 3000ml rolling bottles of digestion until growing up to the good cell lists of 80%-90% 48 hours
Layer, discards cell culture fluid, and the infectious bovine rhinotrachetis virus liquid 25ml of the MDBK cell culture after inactivation is taken to be inoculated in carefully
In dysuria with lower abdominal colic bottle, 37 DEG C are put, containing 5%CO2In incubator, the virus liquid after inactivation is made to adsorb 40min on suitable cell, is during which shaken
It shakes 2 times；
2）After absorption, the DMEM cell maintenance mediums that 175ml contains 3% horse serum are added in cell bottle, cell bottle is placed in 37 DEG C
CO2Continue in incubator culture 3 days after, observe cytopathy, then show to inactivate if any cytopathy it is unqualified, for without thin
The virus liquid of born of the same parents' lesion, then harvest cell culture fluid, and freeze thawing 2 times；
3）The cell culture fluid 15ml after above-mentioned freeze thawing is taken, is inoculated in 175cm2It is 80%-90%'s that square vase, which has grown to density,
MDBK cells on cells after absorption 40min, the cell maintenance medium containing 3% horse serum are added in cell bottle, cell bottle is placed in
37 DEG C of CO2Continue culture 3 days in incubator.Cytopathy, such as acellular lesion are observed, then shows that inactivation is complete.For just
Often do not occur the virus liquid of cytopathy, harvest culture solution, further carry out fluorescent staining.
4）The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in the 96 hole cell trainings for covering with individual layer MDBK cells
It supports in plate, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, is placed in 37 DEG C of CO2In incubator
Continue culture 24 hours, culture solution is discarded, after being cleaned with PBS, with the 0.1mL-20 DEG C of fixation of 80% acetone and 20% methyl alcohol mixed liquor
30min after being cleaned with PBS, is separately added into the BRSV monoclonal fluorescence antibodies 0.1mL of 2.5 μ g/ml, is acted in 37 DEG C of wet box
It after 40min, is cleaned 2 times with PBS, is placed in fluorescence microscopy Microscopic observation result.Positive-virus control wells and normal cell controls hole
Answer unstressed configuration.Test hole is as without specificity fluorescent, then shown that inactivation is complete；Such as there is specificity fluorescent, then inactivation is incomplete.
Claims (2)
1. a kind of inactivation method of inspection suitable for ox BVDV, IBRV, PI3, BRSV inactivated vaccine, it is characterized in that, including following
Step：
1）Selection is suitable for bovine viral diarrhea virus and infectious bovine rhinotrachetis virus, bovine parainfluenza type-3 virus or ox breathing
Road syncytial virus carries out recovery culture in the cell line of suitable growth；
2）2 are selected to grow up to good cell monolayer, density discards cell culture up to the rolling bottle of the appropriate volume of 80%-90%
Liquid takes the virus liquid 20-30ml after inactivation to be inoculated in cell spinner bottle, puts 37 DEG C, containing 5%CO2In incubator, after making inactivation
Virus liquid adsorbs 40-60min on aforementioned suitable cell, during which shakes 2-3 times；
3）After absorption, the cell maintenance medium of certain volume is added in cell spinner bottle, cell spinner bottle is placed in 37 DEG C of CO2Culture
After continuing culture in case 3-4 days, cytopathy is observed, for the virus liquid of acellular lesion, harvests cell culture fluid, and freeze thawing
2-3 times；
4）The cell culture fluid 10-15ml after freeze thawing is taken, is inoculated in 175cm2It is the suitable of 80%-90% that square vase, which has grown to density,
After adsorbing 40-60min on cell, nutrition maintaining liquid is added in cell spinner bottle, cell bottle is placed in 37 DEG C of CO2In incubator
Continue culture 3 days, for may occur in which the virus liquid of cytopathy, observing cytopathy, such as acellular lesion, then showing to have inactivated
Entirely；
5）For not occurring the virus liquid of cytopathy normally, culture solution is harvested, further carries out fluorescent staining, such as without special
Property fluorescence, then show that inactivation is complete；Such as there is specificity fluorescent, then inactivation is incomplete.
2. the inactivation method of inspection according to claim 1 suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine,
It is characterized in, the mode of fluorescent staining is in step 5：The normal virus-culturing fluid for not occurring cytopathy is taken to be inoculated in and covers with individual layer
In 96 porocyte culture plates of MDBK cells, per hole 0.1mL, if each 4 hole of positive-virus control wells and normal cell controls hole, puts
In 37 DEG C of CO2Continue culture 24 hours in incubator, discard culture solution, cleaned with PBS, mixed with 80% acetone and 20% methanol
Liquid 0.1mL fixes 30min at -20 DEG C, is cleaned with PBS, and the ox for being separately added into a concentration of 2.5 μ g/ml corresponds to viral monoclonal
Fluorescence antibody 0.1mL in 37 DEG C of wet box after effect 40min, is cleaned with PBS, is placed in fluorescence microscopy Microscopic observation as a result, positive disease
Unstressed configuration is answered in malicious control wells and normal cell controls hole, and test hole is as without specificity fluorescent, then shown that inactivation is complete；As occurred
Specificity fluorescent, then inactivation is incomplete.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611100655.1A CN108152498A (en) | 2016-12-05 | 2016-12-05 | A kind of inactivation method of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611100655.1A CN108152498A (en) | 2016-12-05 | 2016-12-05 | A kind of inactivation method of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine |
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| CN108152498A true CN108152498A (en) | 2018-06-12 |
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| CN201611100655.1A Pending CN108152498A (en) | 2016-12-05 | 2016-12-05 | A kind of inactivation method of inspection suitable for ox BVDV, IBRV, PIV3, BRSV inactivated vaccine |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593885A (en) * | 2018-12-28 | 2019-04-09 | 苏州药明康德检测检验有限责任公司 | Detection method that is a kind of while detecting ox source virus and pig source virus |
| CN115015549A (en) * | 2022-07-14 | 2022-09-06 | 深圳市卫光生物制品股份有限公司 | Test method for rabies vaccine inactivation verification |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122392A (en) * | 2012-11-12 | 2013-05-29 | 福州大北农生物技术有限公司 | Inactivation inspection method for inactivated vaccine of porcine circovirus |
| CN105734007A (en) * | 2016-03-10 | 2016-07-06 | 金宇保灵生物药品有限公司 | Method of screening MDBK (Madin-Darby Bovine Kidney) cell lines sensitive to bovine viral diarrhea/mucosal viruses |
-
2016
- 2016-12-05 CN CN201611100655.1A patent/CN108152498A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103122392A (en) * | 2012-11-12 | 2013-05-29 | 福州大北农生物技术有限公司 | Inactivation inspection method for inactivated vaccine of porcine circovirus |
| CN105734007A (en) * | 2016-03-10 | 2016-07-06 | 金宇保灵生物药品有限公司 | Method of screening MDBK (Madin-Darby Bovine Kidney) cell lines sensitive to bovine viral diarrhea/mucosal viruses |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109593885A (en) * | 2018-12-28 | 2019-04-09 | 苏州药明康德检测检验有限责任公司 | Detection method that is a kind of while detecting ox source virus and pig source virus |
| CN115015549A (en) * | 2022-07-14 | 2022-09-06 | 深圳市卫光生物制品股份有限公司 | Test method for rabies vaccine inactivation verification |
| CN115015549B (en) * | 2022-07-14 | 2023-01-13 | 深圳市卫光生物制品股份有限公司 | Test method for rabies vaccine inactivation verification |
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Application publication date: 20180612 |