CN108142720A - Broiler chicken high lipid food and its application containing moringa powder - Google Patents
Broiler chicken high lipid food and its application containing moringa powder Download PDFInfo
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- CN108142720A CN108142720A CN201711405856.7A CN201711405856A CN108142720A CN 108142720 A CN108142720 A CN 108142720A CN 201711405856 A CN201711405856 A CN 201711405856A CN 108142720 A CN108142720 A CN 108142720A
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- broiler chicken
- moringa
- lipid food
- high lipid
- feed
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/137—Heterocyclic compounds containing two hetero atoms, of which at least one is nitrogen
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/168—Steroids
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- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
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- Food Science & Technology (AREA)
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- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
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- Physiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
- Meat, Egg Or Seafood Products (AREA)
Abstract
This application provides a kind of broiler chicken high lipid food containing moringa powder, including Moringa feed and high fat raw material, wherein the Moringa feed is mixed by moringa powder and broiler feed.Present invention also provides application of the broiler chicken high lipid food in terms of broiler chicken fatty liver syndrome is prevented, including being raised table hens with the broiler chicken high lipid food.The broiler chicken high lipid food that the application provides contains moringa powder, can both substitute feed a part to reduce production cost, can also prevent broiler chicken disease, and easy to operate, has applications well prospect.
Description
Technical field
The present invention relates to broiler fodders, can especially prevent the broiler chicken high lipid food of broiler chicken fatty liver syndrome and its answer
With.
Background technology
The chicken breed raised at present is mostly using high yield, fast growing as main target, such as high-yield egg chicken and fast large-scale broiler chicken
Deng.But excessive intensive farm and the nutrition supply of superelevation level, lead to the frequent generation of mass-sending property nutrition metabolic diseases, example
Such as broiler chicken fat hepatorenal syndrome.The disease generally betides the broiler of overfertilization, incidence according to chicken kind, strain, daily ration
Composition, environmental factor etc. and it is variant, incidence is usually 1% to 7% or even more than 15%, and the death rate is generally below 5%,
But sometimes up to 20%.Broiler chicken fat hepatorenal syndrome has become the common disease for many national aviculture.The disease is characterized in
Sudden onset, paralysis are then dead.Dissect can find that liver kidney is pale, swelling.
With the fast development of China's aviculture, intensive culture scale constantly expands, which is in the trend increased year by year,
Very big harm is caused to aviculture.It is not clear to the pathogenesis of broiler chicken fat hepatorenal syndrome at present, but and daily ration
Middle nutriment is superfluous and certain micro-nutrient compositions are insufficient or imbalance caused by fat metabolism it is extremely related.It is in addition, living
Chicken sale is constantly restricted, and crosses fattiness, especially abdominal fat, seriously affects the price of chilled chicken sale.
Moringa is a kind of multi-functional tropical plants for having unique economic to be worth and not yet fully develop.Moringa eats history and swings
Long, it is full of nutrition.Gross protein value is 25% or so in Moringa cured leaf powder, vitamin A, B, C, the ore deposits such as E and calcium, magnesium, iron, potassium
Content of material enriches, and anti-nutritional factors is few, is a kind of good vegetable protein feed.Moringa is being raiseeed as feed resource
Application on fowl animal studies have reported that, but have no the defence for the fatty liver class disease for using it for livestock and poultry particularly broiler chicken.
Invention content
To solve the above-mentioned problems, on the one hand, the present invention provides a kind of broiler chicken high lipid food containing moringa powder, packets
Moringa feed and high fat raw material are included, wherein the Moringa feed is mixed by moringa powder and broiler feed.
In preferred embodiments, by weight, the content of the moringa powder for the Moringa feed 5% to
15%.
In a more preferred embodiment, by weight, the content of the moringa powder is the 10% of the Moringa feed.
In a specific embodiment, the moringa powder in dry matter containing 20.75% crude protein, 6.54%
Crude fat and 7.36% crude fibre ash content.
In one embodiment, the high fat raw material includes cholesterol, lard and sucrose.
In another embodiment, the broiler chicken high lipid food further includes propylthiouracil.
In a specific embodiment, by weight, the broiler chicken high lipid food contain 82.5% Moringa feed,
2.0% cholesterol, 10.0% lard, 5.0% sucrose and 0.5% propylthiouracil.
On the other hand, the answering in terms of broiler chicken fatty liver syndrome is prevented the present invention provides the broiler chicken high lipid food
With including being raised table hens with the broiler chicken high lipid food.
The broiler chicken high lipid food of the present invention contains moringa powder, can both substitute feed a part to reduce production cost, may be used also
To prevent broiler chicken disease, and it is easy to operate, there is applications well prospect.
Description of the drawings
Fig. 1 shows the dissection photo of 63 age in days test chickens.A is control group, and B is high in fat group, and C is high in fat group of Moringa.
Fig. 2 shows the liver frozen section photo of 63 age in days test chickens.A is control group, and B is high in fat group, and C is Moringa
High in fat group.
Fig. 3 is that comparison shows block diagram of the test chicken in the hepatic fat content of four different times.
Fig. 4 is that comparison shows block diagram of the test chicken in the abdominal fat of four different times.
Specific embodiment
In order to which technical characteristic, purpose and the advantageous effect to the present invention are more clearly understood, now pass through embodiment pair
Technical scheme of the present invention is further described.
Unless otherwise noted, percentage composition used herein is weight percentage.
" moringa powder " used herein refers to the air-dried powder from leaf of Moringa and/or Moringa stem.Collect 1 meter or so high peppery
Wooden compound stem and leaf, are dried or dries pulverizing, and the Moringa cauline leaf powder of 80-120 mesh is made.In the Moringa cauline leaf powder, dry matter
About 90.16%.20.75% crude protein, 6.54% crude fat and 7.36% can for example be contained in the dry matter
Crude fibre ash content.
" broiler feed " used herein refers to the feed for being commonly used in broiler feeding in the prior art, usually contains jade
The ingredients such as rice, dregs of beans, rapeseed meal, minerals, salt.Those skilled in the art can easily prepare as needed these feeds or
Person is commercially available, such as is bought from Wen Shi groups feed factory.
" Moringa feed " used herein is mixed by moringa powder and broiler feed, wherein typically contain 5% to
15% moringa powder preferably comprises from about 10% moringa powder.
" the broiler chicken high lipid food " of the present invention is formed by the mixed preparings such as Moringa feed and high fat raw material, for example, can contain
There are Moringa feed 82.5%, cholesterol 2.0%, lard 10.0%, sucrose 5.0% and propylthiouracil 0.5%.
Embodiment
1 material
1.1 experimental animal
It is yellow public using 120 42 age in days Wen Shi short-foots, it is provided by Yunfu City Xinxing County Wen Shi broiler chicken hatchery.
1.2 experiment daily rations
Diet is sorghum-soybean meal based diets, is provided by Yunfu City Xinxing County Wen Shi feed factories, and composition is shown in Table 1.
1 normal diet formula of table
1.3 other raw materials
Lard is provided by good hundred million moral Food Co., Ltd；Cholesterol is provided by Henan Wan Bang Industrial Co., Ltd.s；Propylthio
Oxygen pyrimidine is purchased from Hubei Wuhan Dong Kang sources Science and Technology Ltd.；Sucrose is purchased from Shanghai Long Teng You You Food Co., Ltd.
1.4 main experimental instruments
CAH-830 types freezing-microtome (German Lycra)；Spy's optics B-203 types simple microscope (diligent poplar instrument in Guangzhou difficult to understand
Equipment Co., Ltd)；SW-TFG15 type ventilating kitchens；One two-sided clean benches (Purifying Equipment Co., Ltd., Suzhou) of SW-CJ-1F；
PTC-200 type grads PCRs instrument (MJ companies, the U.S.)；Ultralow temperature (- 80 DEG C) refrigerator (REVCO, the U.S.)；General refrigerator (SANYO
Company and HITACH companies, Japan)；Centrifuge5415D types and 5810R types centrifuge (Eppendorf companies, Germany)；
HVE-50 types pressure cooker (HIRAYAMA companies, Japan)；High speed freezing centrifuge (Japanese KUBOTA products).
2 methods
2.1 experimental design
It is yellow 120 public to select 42 age in days Wen Shi short-foots similar in build, is randomly divided into three groups of (control group, high in fat group, Moringas
High in fat group), every group 40, limitation respectively feeds normal diet, high lipid food and Moringa high lipid food.Wherein, normal diet is such as
It is described above, it is provided by Wen Shi feed factories；The composition of high lipid food：Normal diet 82.5%, cholesterol 2.0%, lard
10.0%th, sucrose 5.0%, propylthiouracil 0.5%；The composition of Moringa high lipid food：Moringa feed 82.5% is (with Moringa stem
Leaf powder presses 1 with normal diet:9 mixed preparings), cholesterol 2.0%, lard 10.0%, sucrose 5.0%, propylthiouracil
0.5%.
2.2 feeding managements and clinical observation
Experiment is carried out in Guangdong Wen Shi broiler trials field, is raised chickens and given up using column, test chicken free water, every morning 8:00
With afternoon 17:00 checks henhouse, it is found that dead chicken stops material and weighs, and dead chicken is weighed immediately, timely buried processing.
The state of mind, appetite, morbidity and the death condition of observation test chicken daily, sampling is primary weekly, to sampling chicken, into
Row is dissected, is taken pictures, sample record.
2.3 test indexes measure
2.3.1 liver index determining
Three groups of experiment broiler chicken feed different feeds 21 days respectively, detect the indexs such as weight, feed intake weekly.On-test
0th, 7,14,21 day, every group was randomly selected 5 chickens, after jugular vein blood collection (fasting 12h before blood sampling), is taken liver mass metering and is clapped
According to.Each 5 samples of acquisition (each sample is 3g or so) of every chicken, is put into sterile PBS and cleans, be placed in cryovial, be put into
After liquid nitrogen flash freezer, in -80 DEG C of preservations, for the measure of liver fat rate and making frozen section；Remaining liver takes appropriate hepatic tissue to cut
It is broken, it puts 105 DEG C and dries to constant weight, smash, accurately weigh 3g, wrap that (filter paper is accurate through electronic balance in advance with dry filter paper
Weigh), Soxhlet extractor is put into, with anhydrous ether extracting 12h (bath temperature is 70-80 DEG C), the filter paper packet of degreasing is dried to
Constant weight accurately weighs weight.The following formula acquisition may be used in fat content of liver：Fat content of liver=(filter paper Bao Chong before extraction
Filter paper packet weight after amount-extraction)/(filter paper packet weight-filter weight before extraction) × 100%.
Liver frozen section making step：Freezing microtome is opened, is cooled to -25 DEG C in advance；The tissue of frost is placed in permanent
Freezing cabinet microtome frozen section；Thickness is cut into as 10-20 μm of slice；Slice is pasted onto on glass slide, is air-dried, oil red O stain,
Micro- Microscopic observation.
2.3.2 Biochemical Indices In Serum measures
The the 0th, 7,14,21 day of on-test, every group is randomly selected 5 chickens, jugular vein blood collection (fasting 12h before blood sampling),
Serum is detached, to measure serum TG, TC, LDL-C, ALT, AST.
2.4 data analysis
Test data is mapped using GraphPadPrism softwares, is carried out with Excel2007 through Excel2007 rough estimates
Data preparation, SPSS24.0 are for statistical analysis.As a result it is represented with average value ± standard error, by P<0.05 is used as the significance of difference
Criterion.
3 interpretations of result
Fig. 1 shows the dissection photo of 63 age in days test chickens.After Wen Shi short-foot Huangs cock feeding broiler chicken high lipid food, liver
Significant changes occur for dirty and abdominal fat, and fatty liver shape is presented in liver, and abdominal fat sediment is excessive, and (A is control group, and B is high in fat group, and C is peppery
High in fat group of wood).
Fig. 2 shows that (A is control group, and B is high in fat group, and C is for the liver frozen section photo of corresponding 63 age in days test chicken
High in fat group of Moringa).The liver of freezen protective is taken to cook frozen section, after oil red O stain, can significantly be found, high in fat group, Moringa it is high in fat
The hepatic fat content of group test chicken is significantly higher than control group, but high in fat group of hepatic fat content of Moringa is less than high in fat group, explanation
Moringa powder has apparent prevention effect to broiler chicken fatty liver.
The hepatic fat content comparison and abdominal fat comparison that Fig. 3 and Fig. 4 are shown respectively are consistent with the above results.
The result measured to Biochemical Indices In Serum also indicates that the broiler chicken high lipid food of the invention containing moringa powder can significantly change
The fatty liver index of correlation of kind broiler chicken, the measurement result listed referring to following table 2 to table 6.
Influence (mmol/L) of 2 moringa powder of table to broiler chicken Models of Fatty Liver serum triglyceride (TG) level
Note：Difference is not notable between same letter, and different letters represent significant difference, and following table is identical.
Influence (mmol/L) of 3 moringa powder of table to broiler chicken Models of Fatty Liver serum total cholesterol (TC) level
4 moringa powder of table is to (the influence of (LDL-C) level of broiler chicken Models of Fatty Liver serum LDL cholesterol
(mmol/L)
Influence of 5 moringa powder of table to broiler chicken Models of Fatty Liver serum aspartate amino transferase (AST) level
(mmol/L)
Influence of 6 moringa powder of table to broiler chicken Models of Fatty Liver serum AST/ALT
The present embodiment demonstrates high lipid food by research can lead to broiler chicken fatty liver, and Moringa high lipid food can significantly drop
Low broiler chicken hepatic fat content, reaches significant difference (P after three weeks<0.05), abdominal fat is remarkably decreased (P<0.05).In addition, suffer from
The common several Biochemical Indices In Serums of fatty liver show that high in fat group of Moringa has downward, and TG, LDL-C, AST/ALT reach aobvious
Write difference (P<0.05).
With using compared with normal diet feeding dorking, with high lipid food feeding dorking, advantage is that feed intake declines, feed into
This reduction, production performance improve, but illness rate (fatty liver syndrome), abdominal fat improve, and meat reduces.The present invention use with
Moringa powder substitutes part normal diet and raises table hens, and operating method is easy, not only reduces feed cost, but also has reached and alleviated symptom
Effect.
It should be pointed out that for those of ordinary skill in the art, without departing from the inventive concept of the premise,
Various modifications and improvements can be made, these belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention
It should be subject to the appended claims.
Claims (8)
1. a kind of broiler chicken high lipid food containing moringa powder, including Moringa feed and high fat raw material, wherein the Moringa feed is by peppery
Wood powder and broiler feed mix.
2. broiler chicken high lipid food as described in claim 1, wherein, by weight, the content of the moringa powder is the Moringa
The 5% to 15% of feed.
3. broiler chicken high lipid food as claimed in claim 2, wherein, by weight, the content of the moringa powder is the Moringa
The 10% of feed.
4. broiler chicken high lipid food as described in claim 1, wherein the moringa powder contains 20.75% thick egg in dry matter
In vain, 6.54% crude fat and 7.36% crude fibre ash content.
5. broiler chicken high lipid food as described in claim 1, wherein the raw material high in fat includes cholesterol, lard and sucrose.
6. broiler chicken high lipid food as described in claim 1, wherein further including propylthiouracil.
7. broiler chicken high lipid food as described in claim 1, wherein by weight, the broiler chicken high lipid food contains 82.5%
Moringa feed, 2.0% cholesterol, 10.0% lard, 5.0% sucrose and 0.5% propylthiouracil.
8. application of the broiler chicken high lipid food in terms of broiler chicken fatty liver syndrome is prevented described in any one of claim 1 to 7,
Including being raised table hens with the broiler chicken high lipid food.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711405856.7A CN108142720A (en) | 2017-12-22 | 2017-12-22 | Broiler chicken high lipid food and its application containing moringa powder |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711405856.7A CN108142720A (en) | 2017-12-22 | 2017-12-22 | Broiler chicken high lipid food and its application containing moringa powder |
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| CN108142720A true CN108142720A (en) | 2018-06-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111887347A (en) * | 2020-07-14 | 2020-11-06 | 云南省热带作物科学研究所 | Moringa oleifera leaf powder additive feed for improving intestinal tracts of chickens and preparation method thereof |
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| EP2091352A2 (en) * | 2006-11-13 | 2009-08-26 | Biotec Pharmacon ASA | Animal feeds and veterinary compositions |
| CN105210993A (en) * | 2015-10-30 | 2016-01-06 | 何寒 | A kind of breeding method of Moringa broiler chicken |
| CN105433035A (en) * | 2015-12-22 | 2016-03-30 | 中国农业科学院饲料研究所 | Broiler-chicken feed containing moringa-oleifera leaf powder |
| CN106213024A (en) * | 2016-08-27 | 2016-12-14 | 广西御汇堂生物科技有限公司 | A kind of broiler fodder |
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2017
- 2017-12-22 CN CN201711405856.7A patent/CN108142720A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2091352A2 (en) * | 2006-11-13 | 2009-08-26 | Biotec Pharmacon ASA | Animal feeds and veterinary compositions |
| CN105210993A (en) * | 2015-10-30 | 2016-01-06 | 何寒 | A kind of breeding method of Moringa broiler chicken |
| CN105433035A (en) * | 2015-12-22 | 2016-03-30 | 中国农业科学院饲料研究所 | Broiler-chicken feed containing moringa-oleifera leaf powder |
| CN106213024A (en) * | 2016-08-27 | 2016-12-14 | 广西御汇堂生物科技有限公司 | A kind of broiler fodder |
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| Title |
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| "天路公考"专家团队编: "《畜牧兽医专业知识》", 31 January 2014, 北京：中国铁道出版社 * |
| 习欠云 等: "辣木叶粉对蛋鸡生产性能、蛋品质和血清生化指标的影响", 《饲料添加剂》 * |
| 国务院法制办公室: "《中华人民共和国新法规汇编：行政审批制度改革法规清理专辑三》", 31 July 2017 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111887347A (en) * | 2020-07-14 | 2020-11-06 | 云南省热带作物科学研究所 | Moringa oleifera leaf powder additive feed for improving intestinal tracts of chickens and preparation method thereof |
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Application publication date: 20180612 |