CN108132170A - For the sealing cover of biological respinse - Google Patents
For the sealing cover of biological respinse Download PDFInfo
- Publication number
- CN108132170A CN108132170A CN201711404254.XA CN201711404254A CN108132170A CN 108132170 A CN108132170 A CN 108132170A CN 201711404254 A CN201711404254 A CN 201711404254A CN 108132170 A CN108132170 A CN 108132170A
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- Prior art keywords
- reaction zone
- liquid
- plane
- reaction
- distributor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000007789 sealing Methods 0.000 title claims abstract description 50
- 238000006243 chemical reaction Methods 0.000 claims abstract description 215
- 239000007788 liquid Substances 0.000 claims abstract description 135
- 239000012530 fluid Substances 0.000 claims description 12
- 238000007790 scraping Methods 0.000 claims description 12
- 238000009434 installation Methods 0.000 claims description 2
- 208000012868 Overgrowth Diseases 0.000 claims 1
- 230000008439 repair process Effects 0.000 abstract description 16
- 230000008020 evaporation Effects 0.000 abstract description 4
- 238000001704 evaporation Methods 0.000 abstract description 4
- 239000012295 chemical reaction liquid Substances 0.000 description 11
- 238000010586 diagram Methods 0.000 description 8
- 239000013589 supplement Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000004043 dyeing Methods 0.000 description 3
- 239000005338 frosted glass Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000007792 addition Methods 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 238000001802 infusion Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000006557 surface reaction Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The present invention relates to a kind of sealing covers for biological respinse, its structure includes reaction zone and liquid feeding area, the reaction zone includes reaction zone roof, reaction zone side wall and reaction zone rear wall, the reaction zone roof is being equipped with recess close to reaction zone rear wall position, and reaction zone front end is connect with liquid feeding area by drainage system；During reaction, the reaction zone roof, reaction zone side wall and reaction zone rear wall and micro slide cooperatively form reaction chamber, the reaction zone side wall and reaction zone rear wall and coordinate with the liquid-tight envelope of micro slide, and the indent forms liquid enrichment region.The technical program can reduce during hot repair is multiple because of the influence of reaction solution evaporation and bubble generation to experimental result；The sealing cover additionally aid reaction solution spread out and smoothly, be rapidly introduced into reaction zone；The sealing cover additionally aids cycle of the heat in reaction solution, ensures even heat.
Description
Technical field
The present invention relates to a kind of Bioexperiment devices, particularly a kind of sealing cover for biological respinse.
Background technology
In Bioexperiment, sample is handled by early period, then by by a series of dyeing and remaining processing, makes experimenter can
Clearly to observe the situation of sample.Common processing includes the series of steps such as pretreatment and dyeing, is used under regular situation
Sample tissue is placed on micro slide by manual operation, and by dewaxing, repairing, dyeing and being dehydrated, above-mentioned steps need to will cover
Piece removes carry out filling operation from micro slide, again covers cover plate after operation.This process takes longer, and needs artificial prison
It surveys, therefore occurs can apply to the cover plate that automation means carry out tissue treatment.This kind can be applied to the lid that automation is implemented
Piece, this kind of cover plate front-end and back-end are in communication with the outside, and reaction solution can be made to flow into reaction zone from cover plate front end, and after reaction zone
End outflow, realizes being automatically performed for liquid feeding, cleaning and drain etc., but this kind of cover plate exists when hot repair is multiple and bubble and sky occur
The problem of chamber, this is because hot repair process is longer and temperature needs to reach 100 DEG C, is present in anti-between micro slide and cover plate
Liquid is answered to will appear the situation of bubble and evaporation, and new reaction solution is added in during hot repair again can influence the multiple whole temperature of hot repair
Degree, therefore there is the probability of failure in this kind of cover plate, and heat can be from both ends when heated for this kind of cover plate during hot repair is multiple
It scatters and disappears, leads to thermal loss and the non-uniform situation of heating.
Invention content
The technical problems to be solved by the invention are to provide a kind of sealing cover that can be applied to automation mechanized operation, can reduce
Because of the influence of reaction solution evaporation and bubble generation to experimental result during hot repair is multiple；The sealing cover additionally aids reaction solution
Spread out and smoothly, be rapidly introduced into reaction zone, be further reduced possibility existing for reaction zone hollow cavity or bubble；The sealing cover
Cycle of the heat in reaction solution is additionally aided, ensures even heat, and this follows and badly also contributes to reaction solution in sealing cover
In flowing to reduce possibility existing for reaction zone hollow cavity or bubble.
In addition, the advantages of this sealing cover also has compared to existing cover plate saves reaction solution, and drain is cleaned, facilitated.
Technical solution is used by the present invention solves above-mentioned technical problem：
A kind of sealing cover for biological respinse, structure include reaction zone and liquid feeding area, and the reaction zone includes reaction
Area's roof, reaction zone side wall and reaction zone rear wall, the reaction zone roof are being equipped with recess, reaction close to reaction zone rear wall position
Area front end is connect with liquid feeding area by drainage system；During reaction, the reaction zone roof, reaction zone side wall and reaction zone rear wall with
Micro slide cooperatively forms reaction chamber, the reaction zone side wall and reaction zone rear wall and coordinates with the liquid-tight envelope of micro slide, the recess
Place forms liquid enrichment region.The reaction chamber of only one end open is formed when sealing cover coordinates with micro slide in the technical program, when
When reaction solution enters reaction zone by drainage system, since there are liquid enrichment region, the enrichment regions in reaction zone rear recess
Liquid can generate front end liquid the attraction of bigger so as to which reaction solution be contributed to be full of reaction zone；When hot repair is multiple, notch position
Space is provided for recycle heat, and since three face of chamber is sealed so as to fulfill thermal cycle of the heat in reaction solution to reduce heat
Amount loses and ensures even heat, and when heating causes reaction solution to evaporate and bubble generates, liquid enrichment region stores anti-
Liquid energy is answered to be replenished in time to the region in biochemical reaction, conversion zone production cavity is avoided to influence experimental result, above-mentioned heat
Cycle also promotes the flowing of reaction solution in the reaction region to help the supplement of the discharge of bubble and reaction solution.
Preferably, the recess is connect with reaction zone roof by cambered surface.The connection mode of cambered surface contributes to reaction solution
Into recess and the reaction solution being located in recess and front reaction liquid is made to keep the contact area of bigger to help between larger fluid
Attraction, and heat is also easier to flow into recess with fluid in Thermal Cycling.
Further, the liquid feeding area includes liquid feeding area roof and liquid feeding area side wall, and the liquid zone roof is provided with liquid feeding
Hole, the liquid feeding area roof present position by drain inclined-plane are connect higher than reaction zone roof and with reaction zone roof, described to add
Distributor is additionally provided between fluid apertures and drainage inclined-plane, the distributor has distributor inclined-plane, distributor inclined-plane one end
It is connect with drainage inclined-plane, which is higher than reaction zone roof, and the slope on distributor inclined-plane is less than drainage inclined-plane, distribution
The width on device inclined-plane is less than drainage inclined-plane；Liquid feeding area side wall is connect with reaction zone side wall.The setting of liquid feeding chamber makes this technology
The front-end and back-end of scheme are respectively provided with fluid infusion position, so as to ensure that hot repair it is multiple during reaction solution supplement；The knot of distributor
Structure makes liquid that can quickly flow and can fully spread out when reaching and draining inclined-plane on inclined-plane on it, so as to ensure that
Liquid is replenished in time and will not have air to enter reaction zone during hot repair is multiple.
Further, the dispenser shape approximate trapezoid, width gradually broaden from liquid filling hole to drainage inclined-plane.It is trapezoidal
Structure setting make reaction solution that distributor front end attracts when flowing through distributor with the trend for flowing to both sides, so as to when reaction
It is quickly spread out during liquid contact drainage inclined-plane, so as to which reaction liquid energy be made to be paved with into reaction zone, and since distributor needs to contact
After to a certain amount of reaction solution it can just on distributor inclined-plane flowed, but wide and liquid can be hindered to flow, therefore distribute
It is more suitable that the width of device front end is set as 1.5~2.0mm.
Further, the distributor other end is connect with liquid filling hole, and distributor inclined-plane front end is connected with distributor
Cambered surface, both sides are connected with distributor flank, and the distributor flank inclines from distributor inclined-plane to liquid feeding area roof with drainage inclined-plane
Tiltedly setting, the distributor are connect by distributor cambered surface with liquid filling hole.Distributor connect with liquid filling hole make it easier to instead
Liquid is answered to contact, and distributor is enable to make full use of the distance between liquid filling hole and drainage inclined-plane, so as to make the gradient of distributor
Reduce to increase the flow velocity of reaction solution, and the setting of distributor cambered surface make the reaction solution with distributor contact of incline plane to other
Reaction solution generates attraction, and flows distributor with making it easier to, and the setting of distributor flank makes stream of the reaction solution to both sides
Speed increase, so as to be easier to be spread out on drainage inclined-plane.
Preferably, the angular range of the distributor inclined-plane and plane where micro slide is 5~10 °, the drainage is oblique
The angular range of face and plane where micro slide is 45~60 °.The smaller flow velocity of gradient is faster, but when gradient is too small cannot be guaranteed it is anti-
The exposure of liquid and distributor is answered, therefore the angular range on distributor inclined-plane drainage effect at 5~10 ° is best, and drained oblique
The requirement that face is limited to distance and effectively spreads out, angle answer larger but are to ensure it with preferable drainage effect, therefore drain inclined-plane
Angular range consider at 45~60 ° most preferably.
Preferably, width between two liquid feeding area side walls is more than the width of two reaction zone side walls, liquid feeding area side wall with
Reaction zone side wall is connected by cambered surface.Reaction solution mainly can enter reaction zone other than entering reaction zone from distributor along side wall,
Liquid feeding area side wall is wider than reaction zone side wall and cambered surface is connected as the partial reaction liquid and provides a tangential velocity and flow to angle,
So as to which reaction solution be made to be easier along side wall into reaction zone and be easier to be paved with entire reaction zone.
Preferably, the liquid feeding area further includes and scrapes liquid wall, the liquid wall of scraping is located at liquid filling hole rear end, the liquid wall of scraping
Minimum altitude is higher than reaction zone roof, and the liquid wall of scraping is connect with liquid feeding area roof by cambered surface.Scraping the setting of liquid wall makes drain
When micro slide on liquid can fully be emptied, but this scrape liquid wall it is highly desirable higher than reaction zone roof so as to avoid contact with group
It knits.In addition scrape liquid wall preferably at least have certain height, after avoiding scraping liquid wall haptoreaction liquid, into scrape liquid wall with carry
The reaction solution in gap and the attraction of rear end reaction solution are inadequate between object piece, and fracture occurs so as to which partial reaction liquid be made still to remain
On micro slide, but the height excessively high cannot cause to scrape liquid wall and can not effectively play a role again, according to the situation of reaction solution tension
This scrapes altitude range of the liquid wall apart from reaction zone roof best results between 0.5~1.8mm；And it scrapes liquid wall and leads to liquid feeding area roof
Crossing cambered surface connection makes scraping fluid more smooth, and reaction solution is avoided to remain in sealing cover.
Preferably, when the installation position of the liquid filling hole coordinates sealing cover and micro slide, liquid filling hole is farthest away from reaction
Vertical plane where area is located proximate to or positioned at the rough region of micro slide closest to the vertical plane position of reaction zone.Due to carrying
Object piece has one end rough region (frosted glass), and frictional force is larger so as to cannot be drained well when reacting drop in the region
Device is drawn into reaction zone, and in order to save reaction solution dosage, the position setting of liquid filling hole should avoid reaction solution from entering liquid feeding area
It is contacted afterwards with rough region.
Preferably, extending outwardly on the outside of the two side of sealing cover is provided with locator, one lateral extension of postoperculum is sealed
It stretches and is provided with indicator.The setting of locator enables sealing cover, and in drain, smoothly slip facilitates liquid on entire reaction unit
Body is discharged, and the setting of indicator makes the cooperation of sealing cover and reaction unit unique, avoids user of service by sealing cover mistake
The situation of placement.
The present invention compared with the existing technology has the following advantages and effect：Sealing cover coordinates with micro slide in the technical program
When form the reaction chamber of only one end open, when reaction solution enters reaction zone by drainage system, due at reaction zone rear portion
There are liquid enrichment region in recess, which can generate front end liquid the attraction of bigger so as to contribute to reaction solution
Full of reaction zone；When hot repair is multiple, notch position provides space for recycle heat, and since three face of chamber is sealed so as to fulfill heat
The thermal cycle in reaction solution is measured to reduce thermal loss and ensure even heat, and when heating leads to reaction solution evaporation and gas
When bubble generates, the reaction liquid energy of liquid enrichment region storage is replenished in time to the region in biochemical reaction, and conversion zone is avoided to give birth to
Production cavity to influence experimental result, above-mentioned thermal cycle also promote the flowing of reaction solution in the reaction region with help the discharge of bubble and
The supplement of reaction solution.Recess contributes to reaction solution to enter recess and makes to be located at recessed with reaction zone roof by the connection mode of cambered surface
Reaction solution and front reaction liquid in mouthful keep the contact area of bigger to help the attraction between larger fluid, and thermal cycle
Heat is also easier to flow into recess with fluid in journey.The setting of liquid feeding chamber is respectively provided with the front-end and back-end of the technical program
Fluid infusion position, so as to ensure that hot repair it is multiple during reaction solution supplement；The structure of distributor makes liquid on it can be quick
Flow and simultaneously can fully spread out when reaching and draining inclined-plane on inclined-plane, so as to ensure that hot repair it is multiple during liquid it is timely
It supplements and will not there is air to enter reaction zone.The structure improvement of distributor and drainage inclined-plane is further entered conducive to reaction solution anti-
It answers area and is fully spread out before reaction zone is entered；Equally, the structure setting of reaction zone and liquid feeding area side wall also further functions as
Above effect.The structure setting for scraping liquid wall avoids reaction solution in discharge opeing process from remaining in sealing cover.The position of liquid filling hole ensures
Reaction liquid energy is all used effectively.The setting of locator enables sealing cover smooth on entire reaction unit in drain
Slip facilitates liquid to discharge, and the setting of indicator makes the cooperation of sealing cover and reaction unit unique, and avoiding user of service will
The situation that sealing cover mistake is placed.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
To obtain other attached drawings according to these attached drawings.
Fig. 1 is the dimensional structure diagram of the present invention.
Fig. 2 is the structure diagram of reaction zone side of the present invention.
Fig. 3 is the side sectional view of the present invention.
Fig. 4 is the dimensional structure diagram of reaction zone side of the present invention.
Fig. 5 is the enlarged drawing of reaction zone side of the present invention.
Fig. 6 is the enlarged drawing of another angle in reaction zone side of the present invention
Fig. 7 is the present invention and micro slide cooperation schematic diagram.
Fluid flows to schematic diagram when Fig. 8 is liquid feeding of the present invention.
The liquid that Fig. 9 enters reaction zone for reaction solution of the present invention from liquid feeding area flows to schematic diagram.
Figure 10 is thermal cycle schematic diagram of the present invention.
Figure 11 is the structure diagram when present invention is placed in reaction unit.
Label declaration：
1 reaction zone of sealing cover, 11 reaction zone roof 111
112 reaction zone rear wall of reaction zone side wall, 113 recess 114
12 liquid feeding area roof of liquid feeding area, 121 liquid feeding area side wall 122
Liquid filling hole 123 scrapes liquid wall 124 and drains inclined-plane 13
14 distributor inclined-plane of distributor, 141 distributor cambered surface 142
143 locator of distributor flank, 15 indicator 16
2 frosted glass of micro slide, 21 reaction unit 3
Reaction solution 4
Specific embodiment
With reference to embodiment, the present invention is described in further detail, following embodiment be explanation of the invention and
The invention is not limited in following embodiments.
Embodiment 1：
As shown in Figure 10, the present embodiment forepart is the liquid feeding area 12 for addition or storage reaction liquid, and rear portion is for shape
It into the reaction zone 11 of reaction chamber, is connected between liquid feeding area 12 and reaction zone 11 by draining inclined-plane 13, so as to make liquid feeding area 12
Reaction liquid energy smoothly enters reaction zone 11, and certain drainage system is not limited to this kind drainage inclined-plane 13, such as with the side being oriented to
Wall or roof, the reaction zone inlet with different openings height or to force the fluid into reaction zone etc. by difference in height different
Device form can play this drainage system institute effect to be achieved.With reference to reference to figure 2, reaction zone 11 by reaction zone roof 111,
Reaction zone side wall 112 and reaction zone rear wall 113 limit, and are encircled into semitight reaction chamber with micro slide 2, and the sealing is at least
Including the sealing between reaction zone side wall 112 and reaction zone rear wall 113 and micro slide 2, reaction zone roof 111 is after reaction zone
113 position of wall is equipped with upward recess 114, which may extend to the face of the top of sealing cover, so that reaction solution has more
Big storage capacity, and have the exchange of sufficient space heating load.It is connected, can also led to by cambered surface between the recess 114 and reaction zone roof 111
Cross inclined-plane or right angle connection.When reaction solution 4 enter sealing cover when, flow direction as shown in Figure 8 enter reaction chamber, and
Liquid enrichment region is formed at recess 114；When hot repair is multiple, heat can be formed in reaction chamber as shown in Figure 10 cycle without
It scatters and disappears away from sealing cover back-end.
Embodiment 2：
The present embodiment is substantially the same manner as Example 1, and difference lies in offer through-hole (in figure in the face of the top of sealing cover
It is not shown), which is connected with recess 114, so as to can simultaneously be arranged from the front and rear of sealing cover in hot repair is multiple
Go out bubble without influencing thermal cycle.
Embodiment 3：
As shown in Figures 1 to 4, the present embodiment forepart be for add in or storage reaction liquid liquid feeding area 12, rear portion be for
The reaction zone 11 of reaction chamber is formed, is connected between liquid feeding area 12 and reaction zone 11 by draining inclined-plane 13, reaction zone 11 is by reacting
Area's roof 111, reaction zone side wall 112 and reaction zone rear wall 113 limit, and with reference to reference to figure 8, which closes with micro slide 2
Semitight reaction chamber is surrounded, which includes at least close between reaction zone side wall 112 and reaction zone rear wall 113 and micro slide 2
Envelope, the height of reaction chamber are set as 0.1mm~0.15mm, and volume is 100 microlitres~120 microlitres (not including recess).Reaction
Area's roof 111 is being equipped with upward recess 114 close to 113 position of reaction zone rear wall, and the volume of recess 114 can be set to reacting
Chamber (not including recess) volume is close, and connect between reaction zone roof 111 by cambered surface.With reference to figure 5 to 7, liquid feeding
Area 12 is by liquid feeding area roof 121, liquid feeding area side wall 122 and scrapes liquid wall 124 and forms, height of the liquid zone roof 121 apart from micro slide 2
For 2mm, which is adapted so that reaction solution can adequately be gathered in close to reaction chamber that
One end, liquid feeding area roof 121 offer liquid filling hole 123, and the position selected as of liquid filling hole 123 is close to the frosted glass 21 of micro slide 2
Top but be not in contact with it, can the position away from 12 rear end 3mm~4mm of liquid feeding area, liquid feeding area side wall 122 is arranged on liquid feeding area top
121 both sides of wall, the width between two liquid feeding area side walls 122 are slightly wider than the width between reaction zone side wall 112, and width difference may be configured as
0.15mm, and connected between liquid feeding area side wall 122 and reaction zone side wall 112 by cambered surface, make to enter reaction chamber by side wall
Reaction solution have certain tangential direction speed, the rear end that liquid wall 124 is arranged on liquid feeding area 12 is scraped, apart from reaction zone roof
111 height is 1.5mm, and scrapes and connected by cambered surface between 121 roof of liquid wall 124 and liquid zone roof.Liquid filling hole 123 and drainage
Distributor 14 is additionally provided between inclined-plane 13, distributor 14 is wholely set with liquid feeding area roof 121, and one end is connected to drainage inclined-plane
13 medium position and height apart from reaction zone roof 111 are 1mm, 14 other end of distributor connection liquid filling hole 123, distributor
14 have a distributor inclined-plane 141, and distributor inclined-plane 141 is trapezoidal in one, and the width of forepart and liquid filling hole 123 close to part is
1.7mm, the width at rear portion and drainage inclined-plane 13 close to part is 2.5mm, and 141 front end of distributor inclined-plane is connected with distributor cambered surface
142 and passing through distributor cambered surface 142 and connect with liquid filling hole 123,141 both sides of distributor inclined-plane are connected with distributor flank 142, point
Orchestration flank 142 from distributor inclined-plane 141 to liquid feeding area roof 121 and drainage inclined-plane 13 be obliquely installed, distributor inclined-plane 141 with
The angular range of 2 place plane of micro slide could be provided as 5~10 °, drain inclined-plane 13 and the angle model of 2 place plane of micro slide
It encloses and could be provided as 45~60 °.As shown in FIG. 8 and 9, enter reaction as shown in flow direction after reaction solution enters liquid feeding area 12
Area 11, drainage inclined-plane 13, distributor inclined-plane 141 and side wall cambered surface connection structure ensure that reaction solution smoothly and tile into reaction
Area 11.As shown in Figure 10, cycle is formed without scattering and disappearing away, and damage from sealing cover back-end in reaction chamber when hot repair is multiple
The reaction solution 4 of mistake can supplement at 12 liang of recess 114 and liquid feeding area.
Embodiment 4：
As shown in Figures 1 to 4, the present embodiment forepart be for add in or storage reaction liquid liquid feeding area 12, rear portion be for
The reaction zone 11 of reaction chamber is formed, recess 114 is provided at the rear portion of reaction zone 11 for being enriched with reaction solution in reaction, it is close
Extend outwardly on the outside of the front part sides wall of capping 1 and be provided with locator 15, as shown in figure 11, which puts in sealing cover 1
It puts and plays the role of support and guiding when on reaction unit 3, when needing drain, by locator 15 and reaction unit 3
Slideway cooperation slide backward sealing cover 1.Due to the enrichment of reaction solution, reaction solution at recess 114 on reaction unit 3
Pumping equipment contacts, you can guiding reaction solution discharge is assisted without other equipment, in addition it can in the one end in liquid feeding area 12
Liquid wall 124 is scraped in setting makes it carry out scraping fluid operation in sliding process, makes emptying more complete.The rear portion side of sealing cover 1 is outside
Being extended has indicator 16, and sealing cover 1 is made not malfunction when being placed on reaction unit 3.
Furthermore, it is necessary to illustrate, the specific embodiment described in this specification, the shape of parts and components is named
Title etc. can be different.The equivalent or simple change that all construction, feature and principles according to described in inventional idea of the present invention are done, is wrapped
It includes in the protection domain of patent of the present invention.Those skilled in the art can be to described specific implementation
Example is done various modifications or additions or is substituted in a similar way, without departing from structure of the invention or surmounts this
Range as defined in the claims, is within the scope of protection of the invention.
Claims (10)
1. a kind of sealing cover for biological respinse, including reaction zone and liquid feeding area, it is characterised in that：The reaction zone includes anti-
Area's roof, reaction zone side wall and reaction zone rear wall are answered, the reaction zone roof is being equipped with recess close to reaction zone rear wall position, instead
Ying Qu front ends are connect with liquid feeding area by drainage system；During reaction, the reaction zone roof, reaction zone side wall and reaction zone rear wall
Reaction chamber, the reaction zone side wall and reaction zone rear wall are cooperatively formed with micro slide with the liquid-tight envelope of micro slide to coordinate, it is described recessed
Liquid enrichment region is formed at mouthful.
2. the sealing cover according to claim 1 for biological respinse, it is characterised in that：The recess and reaction zone roof
It is connected by cambered surface.
3. the sealing cover according to claim 1 or 2 for biological respinse, it is characterised in that：The liquid feeding area includes adding
Liquid zone roof and liquid feeding area side wall, the liquid zone roof are provided with liquid filling hole, and the liquid feeding area roof present position is higher than reaction
Area's roof is simultaneously connect with reaction zone roof by draining inclined-plane, and distributor is additionally provided between the liquid filling hole and drainage inclined-plane,
The distributor has distributor inclined-plane, and distributor inclined-plane one end is connect with drainage inclined-plane, and the link position is higher than reaction
Area's roof, and the slope on distributor inclined-plane is less than drainage inclined-plane, and the width on distributor inclined-plane is less than drainage inclined-plane；The liquid feeding
Area's side wall is connect with reaction zone side wall.
4. the sealing cover according to claim 3 for biological respinse, it is characterised in that：The approximate ladder of the dispenser shape
Shape, width gradually broaden from liquid filling hole to drainage inclined-plane.
5. the sealing cover according to claim 4 for biological respinse, it is characterised in that：The distributor other end is with adding
Fluid apertures connects, and distributor inclined-plane front end is connected with distributor cambered surface, and both sides are connected with distributor flank, the distributor side
The wing is obliquely installed from distributor inclined-plane to liquid feeding area roof with drainage inclined-plane, and the distributor passes through distributor cambered surface and liquid filling hole
Connection.
6. the sealing cover according to claim 5 for biological respinse, it is characterised in that：The distributor inclined-plane and loading
The angular range of plane where piece is 5~10 °, and the angular range of the drainage inclined-plane and plane where micro slide is 45~60 °.
7. the sealing cover according to claim 3 for biological respinse, it is characterised in that：Width between two liquid feeding area side walls
More than the width of two reaction zone side walls, liquid feeding area side wall is connect with reaction zone side wall by cambered surface.
8. the sealing cover according to claim 3 for biological respinse, it is characterised in that：The liquid feeding area further includes scraping fluid
Wall, the liquid wall of scraping are located at liquid filling hole rear end, and the minimum altitude for scraping liquid wall is higher than reaction zone roof, and the liquid wall of scraping is with adding
Liquid zone roof is connected by cambered surface.
9. the sealing cover according to claim 3 for biological respinse, it is characterised in that：The installation position of the liquid filling hole
When making sealing cover with micro slide cooperation, the vertical plane where liquid filling hole farthest away from reaction zone is located proximate to or positioned at micro slide
Rough region is closest to the vertical plane position of reaction zone.
10. the sealing cover according to claim 1 or 2 for biological respinse, it is characterised in that：Outside the two side of sealing cover
Epitaxial lateral overgrowth, which is stretched, is provided with locator, and sealing postoperculum side, which extends outwardly, is provided with indicator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711404254.XA CN108132170A (en) | 2017-12-22 | 2017-12-22 | For the sealing cover of biological respinse |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711404254.XA CN108132170A (en) | 2017-12-22 | 2017-12-22 | For the sealing cover of biological respinse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108132170A true CN108132170A (en) | 2018-06-08 |
Family
ID=62391432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711404254.XA Pending CN108132170A (en) | 2017-12-22 | 2017-12-22 | For the sealing cover of biological respinse |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108132170A (en) |
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| CN106537223A (en) * | 2014-07-09 | 2017-03-22 | 文塔纳医疗系统公司 | Automated coverslipper and methods of use |
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2017
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